阅读说明：本技术 修饰的核苷或核苷酸 (Modified nucleosides or nucleotides ) 是由 刘二凯 陈奥 章文蔚 于 2017-10-11 设计创作，主要内容包括：本发明涉及核酸测序领域。特别地,本发明提供了一种修饰的核苷或核苷酸,所述修饰的核苷或核苷酸的3’-OH被可逆阻断,并且,所述修饰的核苷或核苷酸携带有可检测标记。本发明还涉及包含所述核苷或核苷酸的试剂盒,制备所述核苷或核苷酸的方法,以及基于所述核苷或核苷酸的测序方法。(The present invention relates to the field of nucleic acid sequencing. In particular, the present invention provides a modified nucleoside or nucleotide having a reversible blockade of the 3' -OH and carrying a detectable label. The invention also relates to a kit comprising said nucleoside or nucleotide, a method for preparing said nucleoside or nucleotide, and a sequencing method based on said nucleoside or nucleotide.)