阅读说明：本技术 金雀花提取物的应用 (Application of gorse extract ) 是由 宋肖洁 姜丹 姚期凤 王越 蒋耀权 吴越 章漳 于 2018-06-29 设计创作，主要内容包括：本发明属于植物提取及日化领域,具体涉及金雀花提取物的应用,所述金雀花提取物作为皮肤外用剂中的抗衰老活性成分,具体为皮肤修复活性成分、和/或皮肤结构稳固活性成分、和/或皮肤弹性活性成分,具有提高细胞修复/增殖、稳固基底膜结构和功能、促进细胞外基质分泌、保持皮肤弹性的效果。本发明金雀花提取物活性高,在含量极低的情况下即产生显著效果,天然、安全且有效,不刺激皮肤。(The invention belongs to the field of plant extraction and daily chemicals, and particularly relates to an application of a gorse extract, wherein the gorse extract is used as an anti-aging active ingredient in a skin external preparation, particularly a skin repair active ingredient, and/or a skin structure stabilizing active ingredient, and/or a skin elasticity active ingredient, and has the effects of improving cell repair/proliferation, stabilizing a basement membrane structure and function, promoting extracellular matrix secretion, and keeping skin elasticity. The broom extract has high activity, can generate obvious effect under the condition of extremely low content, is natural, safe and effective, and does not irritate the skin.)

1. Use of a broom extract as an anti-aging active ingredient in the preparation of a skin external preparation is characterized in that the broom extract is used as an anti-aging active ingredient in the skin external preparation.

2. Use according to claim 1, characterized in that the extract of the broom (sparrow) achieves anti-ageing activity as active ingredient for skin repair, and/or for stabilizing the structure of the skin, and/or for maintaining or enhancing the elasticity of the skin.

3. Use according to claim 2, characterized in that the extract of the broom (sparrow) performs the function of skin repair as cell repair and/or proliferation active ingredient;

the gorse extract is used as an active ingredient for stabilizing the structure and the function of a basement membrane to stabilize the structure of the skin;

the flos Caraganae Sinicae extract is used as active ingredient for promoting extracellular matrix secretion to maintain or enhance skin elasticity.

4. Use according to claim 3, characterized in that the extract of the broom cypress is used as an active ingredient for increasing the expression of genes related to cell repair and/or proliferation, and/or for increasing the expression of genes related to basement membrane, and/or for increasing the expression of genes related to skin elasticity.

5. The use of claim 4, wherein the cell repair and/or proliferation-related gene is the SIRT1 gene, HBEGF or IGF1 gene, the basement membrane-related gene is the col4 α 1 gene, and the skin elasticity-related gene is the ELN gene.

6. Use according to claim 3, wherein the extract of Genistein enhances cell repair and/or proliferation by increasing the synthesis of histone deacetylase, and/or heparin-binding epidermal growth factor, and/or insulin-like growth factor 1; the gorse extract is used as an active component for synthesizing IV type collagen alpha 1 chain to enhance the structure and the function of a basement membrane; the flos Caraganae Sinicae extract can be used as elastin synthesis active ingredient for promoting extracellular matrix secretion, and/or stabilizing skin elasticity.

7. The use according to any one of claims 1 to 6, wherein the preparation method of the broom extract comprises: taking the broom as a raw material, extracting with a solvent, and taking a filtrate.

8. A skin external preparation having skin-repairing and/or skin-firming activity, and/or skin-elasticity activity, and/or anti-aging activity, characterized by comprising a maidenhair extract.

9. The external preparation for skin as claimed in claim 8, wherein the extract of broom cypress is used in an amount of 0.0001 to 10 wt%.

10. The external preparation for skin according to claim 8 or 9, further comprising one or more of a moisturizing active ingredient, a whitening active ingredient, a moisturizing active ingredient, an antioxidant active ingredient, an anti-wrinkle active ingredient, a spot-removing active ingredient, an acne-removing active ingredient, a sunscreen active ingredient, an acne/acne-removing active ingredient, a dandruff-removing active ingredient, an antiallergic active ingredient, or a sebaceous gland-inhibiting active ingredient.

Technical Field

The invention belongs to the field of plant extraction and daily chemicals, and particularly relates to an application of a gorse extract.

Background

The skin covers the whole human body surface and is in direct contact with the external environment, so that the mirror not only protects the body from being damaged by various external factors, but also reflects the health state of the body. The skin is composed of epidermis, dermis, and subcutaneous tissue, and contains skin appendages, abundant nerves, blood vessels, lymphatic vessels, and muscles. The connecting area of the dermis and the epidermis is called as a basement membrane zone, the basement membrane is a supporting pad of epithelial cells, has supporting and connecting effects between epithelial cell tissues and connective tissues, and is also a semipermeable membrane with material permeability, so that the material exchange is facilitated. The basement membrane not only serves as a layer of structural model for supporting epidermal cells, but also serves as a selective passing barrier of cells, preventing connective tissue cells from contacting epithelial cells on the basement membrane, but allowing lymphocytes, macrophages and neuronal synapses to pass through; many vital phenomena such as cell morphology, cell polarity, cell metabolism, protein distribution on the plasma membrane, cell survival, cell proliferation and differentiation, and cell migration are very closely related to the basement membrane.

The dermis is composed of cells and extracellular matrix, fibroblasts are main cells of the dermis, and the normal proliferation and differentiation of the fibroblasts maintain the structure of the dermis of the skin to form a normal structure and physiological function; extracellular matrix is a kind of macromolecular substance synthesized and secreted by cells and interacting around the cells, and many life phenomena such as proliferation and differentiation of cells, cell migration and the like are closely related to the extracellular matrix. The extracellular matrix is composed of four major classes of molecules: collagen, elastin, glycoprotein and proteoglycan. The extracellular matrix is finely and skillfully entangled and interwoven through different molecules to form various exquisite three-dimensional structures, so that the skin is endowed with elasticity and tensile strength, and looks plump and full of vitality.

The longevity gene is a gene capable of keeping active for a long time, and can greatly improve the health of organisms and prolong the life of the organisms. When a person encounters severe cold, heat or water and food deficient environments, these genes can activate the self-defense system in the human body, making the cell repair capacity powerful.

SIRT1(sirtuin 1): one of the famous longevity genes. SIRT1 is a highly conserved protein deacetylase, is involved in regulating the longevity caused by energy restriction, is called a longevity gene, has complex physiological actions of SIRT1, and is involved in regulating glycolipid metabolism, inflammation, oxidative stress, cell senescence, apoptosis and the like.

The HBEGF (heparin binding EGF like growth factor) gene encodes a synthetic heparin binding epidermal growth factor, which is an EGF family member that binds to and activates EGF receptors. hbegf plays a key role in pathological and physiological processes, including cancer. After the skin part of the human body is damaged, the epidermal growth factor is produced, the proliferation and the homing of cells (comprising keratinocytes, dermal cells and basement membrane cells) are promoted, and the healing of skin wounds is promoted. The up-regulation of the gene expression is helpful for promoting the repair of skin and delaying skin aging.

The col4 alpha 1(collagen type IV alpha 1 chain) gene encodes and synthesizes type IV collagen alpha 1 chain, type IV collagen is the main component forming basement membrane, and the up-regulation of the gene expression can promote the integrity of the basement membrane (also called as dermoepidermal junction, DEJ) and delay skin aging.

The IGF1(insulin like growth factor 1) gene encodes synthetic insulin-like growth factor 1, a protein that is functionally and structurally similar to insulin and is a member of a family of proteins involved in regulating growth and development. Insulin-like growth factor 1 promotes keratinocyte migration and re-epithelialization of the wound surface.

The ELN (elastin) gene encodes synthetic elastin, which is the main component of elastic fibers (elastic fibers) and is rich in hydrophobic amino acids, such as glycine and proline, and elastin molecules are cross-linked to each other by covalent bonds between lysine residues, and the formed cross-linked network can generate elasticity through configuration change. Elastin is an important extracellular matrix in skin and is closely related to dermal aging, which is a ring of importance in causing skin aging. Elastin acts as a rubber band in the skin, allowing the skin to stretch and fold, and functions like a spring in a mattress to maintain and support the elasticity of the skin. Therefore, elastin plays an important role in maintaining skin elasticity.

Therefore, the research on how to improve the expression of genes related to key parts and functions of the skin has important significance on improving the skin repair capacity, keeping the structural stability and delaying the skin aging.

Genistein (Latin name: Parochetus communis) also known: the sparrow flower is creeping herb of sparrow of Leguminosae, 10-20 cm high, and sparse and soft. The rootstock is filiform, the root is rooted on the node, and the root nodule is formed. The tip of the petal is slightly sharp, and the side of the petal is divided into two petals, the trend is like flying sparrow, the color is golden yellow, so the petal is named as 'gold sparrow'. Grows on grassland, hillside and wasteland beside the road, and has the elevation of 2000-3000 meters. Sichuan, Yunnan and Tibet. India, nipal, Plumbum preparatium, Srilanka, Burma, Thailand, Malaysia and eastern Africa are also distributed.

Xiguang, it usually occurs on hillside and faces the sun. The root system is developed, has root nodules, resists drought and barren and can grow in gaps of the hills and stones. Avoid wet waterlogging. Strong germination and tillering power, and can be naturally sown and propagated. Better growth in deep, fertile and moist sandy loam.

The broom has mild nature and sweet taste, enters liver and spleen meridians; has effects in nourishing yin, regulating blood, and invigorating spleen; it can be used for treating cough due to excessive heat, dizziness, soreness of waist, leukorrhagia due to qi deficiency, infantile malnutrition, acute mastitis, and traumatic injury. Root: nourishing, strengthening, promoting blood circulation, regulating menstruation, dispelling pathogenic wind, and removing dampness. Can be used for treating hypertension, dizziness, tinnitus, dim eyesight, asthenia, menoxenia, leucorrhea, galactostasis, rheumatic arthralgia, and traumatic injury. Flower: dispel wind, activate blood, relieve cough and resolve phlegm. Can be used for treating dizziness, tinnitus, cough due to lung deficiency, and infantile dyspepsia.

The flos Caraganae Sinicae contains protein, fat, carbohydrate, vitamins, minerals, etc. The wild herb broom cypress flower has mild sweet taste, and has the effects of nourishing yin, regulating blood and tonifying spleen. It can be used for treating cough due to excessive heat, dizziness, soreness of waist, asthma, leucorrhea, infantile malnutrition, acute mastitis, and traumatic injury. The book of drawings and examinations of various plants carries on "nourishing yin and tonifying yang". Shanghai commonly used Chinese herbal medicine carries the functions of promoting blood circulation, dispelling wind, relieving cough and strengthening body. It is used to treat dizziness, headache, tinnitus, dim eyesight, lung deficiency, chronic cough, and infantile malnutrition. The nature and flavor of the herbs are sour, astringent and warm.

The invention proves that the broom extract can improve the expression of cell repair related genes, basement membrane stability related genes and anti-aging related genes, improve the cell repair activity, stabilize the structure and function of the basement membrane, promote the secretion of extracellular matrix and delay skin aging for the first time, and is suitable for preparing skin external preparations.

Disclosure of Invention

The invention aims to provide application of a gorse extract.

The flos Caraganae Sinicae extract has effects of improving skin repairing activity, stabilizing skin structure, promoting extracellular matrix secretion, maintaining skin elasticity, and delaying skin aging, and is suitable for preparing skin external preparation.

The sparrow flower extract enhances the cell repair activity by improving the expression of cell repair/proliferation related genes, so as to improve the skin repair activity and delay skin aging; the broom cypress extract enhances the structure and the function of the basement membrane by improving the expression of the genes related to basement membrane stability so as to stabilize the structure of the skin and delay skin aging; the caragana sinica franch enhances the cell repair and/or proliferation activity, and/or basement membrane structure and function, and/or extracellular matrix secretion, and/or maintains the skin elasticity and delays the skin aging by improving the expression of anti-aging related genes.

The broom extract can be used for preparing skin external preparations, is used as an anti-aging active ingredient in the skin external preparations, and particularly is used as a skin repair active ingredient and/or a skin structure stabilizing active ingredient and/or an active ingredient for maintaining skin elasticity to realize an anti-aging effect.

The broom extract is used as an active component for promoting cell repair and/or proliferation, and can enhance the skin repair activity by promoting the proliferation and repair of cells, particularly dermal cells or epidermal cells, realize the skin repair function and delay skin aging.

The broom extract is used as a basement membrane structure and functional stabilizing active ingredient, stabilizes the skin structure and delays skin aging.

The broom extract is used as an extracellular matrix secretion active component to maintain or enhance skin elasticity and delay skin aging.

The broom extract is used as an active ingredient for improving the expression of cell repair and/or proliferation related genes, and/or an active ingredient for improving the expression of basement membrane related genes, and/or an active ingredient for improving the expression of skin elasticity related genes. Preferably, the cell repair and/or proliferation related gene is SIRT1 gene, HBEGF gene or IGF1 gene, the basement membrane related gene is col4 α 1 gene, and the skin elasticity related gene is ELN gene.

The broom extract enhances cell repair and/or proliferation activity by increasing the expression of the SIRT1 gene to promote synthesis of histone deacetylase, by increasing the expression of the HBEGF gene to promote synthesis of heparin binding epidermal growth factor, and by increasing the expression of the IGF1 gene to promote synthesis of insulin-like growth factor 1.

The gorse extract can improve the expression of col4 alpha 1 gene, promote the synthesis of IV type collagen alpha 1 chain, and enhance the structure and function of basement membrane.

The sparrow flower extract promotes elastin synthesis by improving ELN gene expression, thereby promoting extracellular matrix secretion and maintaining skin elasticity.

The broom extract refers to an extract containing effective components obtained by extracting in any reasonable way.

The preparation method of the broom extract comprises the following steps: taking the broom as a raw material, extracting with a solvent, and taking a filtrate. The extraction solvent comprises water, alcohol or alcohol water solution, preferably 60-85% (volume ratio) ethanol, and more preferably 70% ethanol.

Extracting for 1-3 times, preferably 2 times; the extraction time is 20-80 minutes, preferably 30 minutes.

The extraction method comprises ultrasonic method, leaching method, supercritical method, percolation method, reflux method or distillation method, wherein the ultrasonic method, leaching method and percolation method are carried out at room temperature. The extraction method is preferably ultrasonic extraction.

The dosage ratio of the broom to the solvent is 1 kg/5-20L in each extraction, and the preferred dosage ratio is 1 kg/10L.

The filtrate may be further concentrated or concentrated to dryness.

The concentration method is vacuum concentration.

The concentrating and drying method is vacuum drying or freeze drying.

The broom is subjected to crushing treatment before extraction, and the particles are crushed into particles with the particle size of 20-300 meshes, and more preferably 50-200 meshes. Preferably, the broom is dried to a moisture content of less than 10 wt%.

The broom refers to the flower, whole grass, root and rhizome of the broom or a mixture thereof, and is preferably the flower of the broom.

In a preferred embodiment of the present invention, the extract of broom is prepared by mixing pulverized broom with 70% ethanol (volume concentration) at a ratio of 1kg/10L, and ultrasonically extracting at room temperature for 30min for 2 times; the filtrates were combined, the solvent removed and dried.

The extract of the broom (canary flower) can be used for preparing a skin external preparation, and is used as an anti-aging active ingredient in the skin external preparation, in particular an active ingredient for repairing skin, an active ingredient for stabilizing skin structure or an active ingredient for maintaining skin elasticity.

The broom extract can be used for preparing skin external preparations for enhancing the expression of SIRT1 gene and promoting the synthesis of histone deacetylase, for preparing skin external preparations for enhancing the expression of HBEGF gene and promoting the synthesis of heparin-binding epidermal growth factor, for preparing skin external preparations for enhancing the expression of IGF1 gene and promoting the synthesis or insulin-like growth factor 1, and for enhancing cell repair and/or proliferation activity.

The broom extract can be used for preparing skin external preparations for enhancing col4 alpha 1 gene expression and promoting IV type collagen alpha 1 chain synthesis, and can enhance basement membrane structure and function.

The flos Caraganae Sinicae extract can be used for preparing skin external preparation for enhancing ELN gene expression, promoting elastin synthesis, promoting extracellular matrix secretion, and maintaining skin elasticity.

The broom extract can promote skin repair and delay skin aging by improving the expression of cell repair related genes.

The sparrow flower extract stabilizes a basement membrane structure and enhances true epidermal junction by enhancing the expression of basement membrane related genes (such as col4 alpha 1 gene), thereby stabilizing a skin structure and realizing anti-aging; on the other hand, the composition promotes the generation and the secretion of extracellular matrix, strengthens and maintains the elasticity of the skin, thereby delaying the skin aging.

A skin external preparation has skin antiaging activity, especially skin repairing activity, and/or skin function stabilizing activity, and/or skin elasticity improving activity, and contains flos Caraganae Sinicae extract. Wherein, the content of the broom extract is 0.0001 wt% -10 wt%, preferably 0.0005 wt% -10 wt%; more preferably 0.001 to 5 wt%.

Furthermore, when the extract is used for improving the expression of the SIRT1 gene, the HBEGF gene or the col4 alpha 1 gene, the content of the broom extract is 0.0005 wt% -10 wt%, preferably 0.001 wt% -5 wt%; when the extract is used for improving the expression of IGF1 gene or ELN gene, the content of the broom extract is 0.005 wt% -10 wt%, preferably 0.01 wt% -5 wt%.

The skin external agent refers to a product which is spread on any part of the surface of the human body by smearing, spraying or the like, so as to clean, maintain, beautify, modify and change the appearance, or modify the smell of the human body and keep the good state. The skin external preparation can be basic cosmetics, facial cosmetics, head care products, and body care products for cleaning, caring, beautifying or caring skin.

The formulation of the skin external preparation is not particularly limited, and may be determined according to the use condition, such as cleansing cream, essence, lotion, cream, lotion (such as smoothing toner, toner), mask, makeup remover, gel, aerosol or spray.

The skin external preparation containing the broom cypress extract can also contain other active ingredients, including one or more of water supplementing active ingredients, whitening active ingredients, moisturizing active ingredients, antioxidant active ingredients, anti-wrinkle active ingredients, freckle removing active ingredients, acne removing active ingredients, sunscreen active ingredients, acne/acne removing active ingredients, dandruff removing active ingredients, antiallergic active ingredients or sebaceous gland inhibiting active ingredients.

The skin external preparation further comprises one or more of thickener, solubilizer, cosolvent, antiseptic, surfactant, stabilizer, softener, aerosol solvent, aromatic, toner, penetration enhancer, controlled release agent, sustained release agent, humectant, emulsifier, shaping agent or pearling agent.

Compared with the prior art, the invention has the following advantages:

(1) the broom extract can improve the expression of cell repair and proliferation related genes, basement membrane stability related genes and skin elasticity related genes, has the effects of improving cell repair/proliferation activity, stabilizing basement membrane structure and function, promoting extracellular matrix secretion, keeping skin elasticity and the like, and can repair skin function, stabilize skin structure, enhance and maintain skin elasticity, thereby delaying skin aging. The broom extract has anti-aging effect, and has wide application prospect in skin external preparations, especially in cosmetics.

(2) The preparation process of the broom extract is simple, the process conditions are easy to control, and the preparation cost is low.

(3) The broom extract has high activity, can generate obvious effect under the condition of extremely low content, is natural, safe and effective, and does not irritate the skin.

Drawings

FIG. 1 shows the result of blank control (NT) and maidenhair extract promoting SIRT1 gene expression.

FIG. 2 shows the results of the blank control (NT) and the extract of Genistis canadensis promoting HBEGF gene expression.

FIG. 3 shows the results of the blank control (NT) and the extract of Genistis canadensis promoting the expression of col4 alpha 1 gene.

FIG. 4 shows the results of the blank control (NT) and the extract of Genistis canadensis promoting the expression of IGF1 gene.

FIG. 5 shows the results of ELN gene expression promotion by the blank control (NT) and the extract of Caragana sinica Linn.

Detailed Description