阅读说明：本技术 一种快速提取甜茶苷的方法 (Method for rapidly extracting rubusoside ) 是由 尹成男 于 2019-10-10 设计创作，主要内容包括：本发明公开了一种快速提取甜茶苷的方法,包括粉碎、过筛——提取——脱色——脱涩、脱苦——结晶、重结晶等步骤,本发明的提取方法能够快速的将甜茶中的甜茶苷提出取来,且甜茶苷提取率高,成本低,能够满足大规模的低成本的提取。(The invention discloses a method for rapidly extracting rubusoside, which comprises the steps of crushing, sieving, extracting, decoloring, deastringency, debitterizing, crystallizing, recrystallizing and the like.)

1. A method for rapidly extracting rubusoside is characterized by comprising the following steps,

1) crushing and sieving: crushing the dried sweet tea leaves, and sieving the crushed sweet tea leaves by a 40-mesh sieve to obtain powder A;

2) extraction: placing the powder A in a water tank connected with an ultrasonic generator, carrying out ultrasonic treatment for 10-15 minutes, filtering with a filter membrane to obtain filtrate and filter residue, carrying out twice distilled water washing on the filter residue and filtering with the filter membrane to obtain twice water washing filtrate, and mixing the filtrate and the twice water washing filtrate to obtain filtrate B;

3) and (3) decoloring: decolorizing and adsorbing the filtrate B by a macroporous adsorption resin column to obtain decolorized solution, cleaning the macroporous adsorption resin column by distilled water until no sweet taste exists in water washing to obtain decolorized cleaning solution, and mixing the decolorized solution and the decolorized cleaning solution to obtain mixed solution C; regenerating the macroporous adsorption resin column after being cleaned without sweet taste;

4) removing astringent taste and bitter taste: adding active carbon into the mixed solution C, stirring, filtering, concentrating under reduced pressure, and spray drying to obtain rubusoside crude product;

5) and (3) crystallization and recrystallization: adding a solvent into the rubusoside crude product, placing the mixture in a water bath at 75-85 ℃ to be heated until the mixture is completely dissolved, filtering the mixture while the mixture is hot, standing the mixture to separate out, filtering the mixture, recrystallizing the mixture, and performing spray drying to obtain a rubusoside refined product.

2. The method for rapidly extracting rubusoside according to claim 1, wherein distilled water with the temperature of 85-90 ℃ is filled into the ultrasonic water tank, and the water-powder ratio is 20-30: 1.

3. The method for rapidly extracting rubusoside according to claim 1, wherein the solvent is one or more of methanol, ethanol, acetone, ethyl acetate, chloroform or petroleum ether.

Technical Field

The invention relates to a method for rapidly extracting rubusoside, belonging to the field of biochemistry.

Background

The sweet tea is a perennial vine of Rosaceae, is a wild rare sweet plant with no toxicity, low heat energy, high sweetness and health care function which is unique to Guangxi, and is mainly produced in Yaoshan, Jinxiu, Yongfu, Cenxi and other counties and cities of Guangxi. Because of its sweet taste, it is named sweet tea. The three sweet plants of Guangxi, which are called together with fructus momordicae and stevia rebaudiana, have a long history of application among Guangxi people, and local people can drink the three sweet plants as tea for a long time, and the three sweet plants are also used for processing foods instead of sugar and can be used as medicines.

The main components of sweet tea comprise sweet tea glycoside, bioflavonoid, tea polyphenol, amino acid, inorganic salt, etc. The main sweet component of folium hydrangeae strigosae is rubusoside, which is tetracyclic diterpene glycoside formed by combining steviol and glucose, has a molecular formula of C20H50O13, and is similar to stevioside in chemical structure. Rubusoside has about 300 times the sweetness of sucrose, while the caloric value is only 5% of sucrose. Pharmacological experiments show that the rubusoside has the characteristics of reducing blood sugar, reducing blood pressure, promoting metabolism, inhibiting hyperacidity, clearing heat, moistening lung, promoting fluid production, quenching thirst, high sweetness and low calorific value, can be used as a medicine, is a food additive with excellent quality, and can replace cane sugar to be used in foods, health-care products and medicines for patients with hypertension, diabetes, obesity and the like, old people and children.

With the wider application of the sweet glycosides, more and more methods for extracting the sweet glycosides from the sweet tea are provided, some of the methods pay attention to extraction rate, some pay attention to extraction efficiency, some pay attention to extraction cost and the like, and in short, no extraction method in the existing extraction method can take the aspects into consideration, so that the extraction of the sweet tea glycosides is severely restricted.

Disclosure of Invention

The invention aims to provide a method for quickly extracting rubusoside, which can quickly extract rubusoside from rubus suavissimus and can meet the requirement of large-scale low-cost extraction.

The invention provides a method for rapidly extracting rubusoside, which comprises the following steps,

1) crushing and sieving: crushing the dried sweet tea leaves, and sieving the crushed sweet tea leaves by a 40-mesh sieve to obtain powder A;

2) extraction: placing the powder A in a water tank connected with an ultrasonic generator, carrying out ultrasonic treatment for 10-15 minutes, filtering with a filter membrane to obtain filtrate and filter residue, carrying out twice distilled water washing on the filter residue and filtering with the filter membrane to obtain twice water washing filtrate, and mixing the filtrate and the twice water washing filtrate to obtain filtrate B;

3) and (3) decoloring: decolorizing and adsorbing the filtrate B by a macroporous adsorption resin column to obtain decolorized solution, cleaning the macroporous adsorption resin column by distilled water until no sweet taste exists in water washing to obtain decolorized cleaning solution, and mixing the decolorized solution and the decolorized cleaning solution to obtain mixed solution C; regenerating the macroporous adsorption resin column after being cleaned without sweet taste;

4) removing astringent taste and bitter taste: adding active carbon into the mixed solution C, stirring, filtering, concentrating under reduced pressure, and spray drying to obtain rubusoside crude product;

5) and (3) crystallization and recrystallization: adding a solvent into the rubusoside crude product, placing the mixture in a water bath at 75-85 ℃ to be heated until the mixture is completely dissolved, filtering the mixture while the mixture is hot, standing the mixture to separate out, filtering the mixture, recrystallizing the mixture, and performing spray drying to obtain a rubusoside refined product.

Preferably, distilled water with the temperature of 85-90 ℃ is filled into the ultrasonic water tank, and the water-powder ratio is 20-30: 1.

Preferably, the solvent is one or more of methanol, ethanol, acetone, ethyl acetate, chloroform or petroleum ether.

The method for rapidly extracting rubusoside according to the technical scheme of the invention has the beneficial effects that:

1. the ultrasonic extraction is utilized, the extraction speed is high, the efficiency is high, and the method is suitable for large-scale production.

2. No chemical agent is adopted in the extraction process, the cost is low, and the method is suitable for large-scale industrial production.

3. The extraction steps are few, the extraction efficiency is high, the technical requirements on operators are low, and the method can be rapidly popularized and applied.

Detailed Description

In order to facilitate the understanding of the technical solutions of the present invention for those skilled in the art, the technical solutions of the present invention will now be further described with reference to specific embodiments.