阅读说明：本技术 微流体器件 (Microfluidic device ) 是由 今村一彦 乾延彦 小原正太郎 河野隆昌 高松辰典 石井亮马 于 2018-05-25 设计创作，主要内容包括：本发明提供一种微流体器件,其能够可靠地进行流体到分支流路中的称取和预定量的流体到多个分支流路中的分注。本发明涉及微流体器件(11),其中,微流路(11)具有主流路(12)和分支流路(15)～(17),主流路(12)具有第一流路扩大部(12d),分支流路(15)～(17)具有第二流路扩大部(15c)～(17c),就下述式(1)中所示的T值而言,作为所述分支流路的T值的TB值与作为所述主流路的T值的TE值之差(TB-TE)为5以上,T＝{1/(x<Sup>2</Sup>·R)}·(θ/90) 式(1)。需要说明的是,在式(1)中,x是第一、第二流路扩大部的起点处的流路宽度,R是第一、第二流路扩大部中的曲面状部分的曲率半径。θ表示以第一、第二流路扩大部的起点以及流路扩大部的终点为端部且曲率半径为R的圆弧所对应的中心角。(The invention provides a microfluidic device capable of reliably weighing a fluid in a branch channel and dispensing a predetermined amount of fluid into a plurality of branch channels. The present invention relates to a microfluidic device (11), wherein the microfluidic channel (11) has a main channel (12) and branch channels (15) to (17), the main channel (12) has a first channel expansion section (12d), the branch channels (15) to (17) have second channel expansion sections (15c) to (17c), the difference (TB-TE) between the TB value as the T value of the branch channel and the TE value as the T value of the main channel is 5 or more, and T is { 1/(x)/[ T ] } 2 R) } (theta/90) formula (1). In the formula (1), x is the channel width at the starting point of the first and second channel enlarging portions, and R is the radius of curvature of the curved portion in the first and second channel enlarging portions. θ represents a central angle corresponding to an arc having a radius of curvature R and having a start point of the first and second expanded flow passages and an end point of the expanded flow passage as end portions.)

1. A microfluidic device having an injection-molded body containing a synthetic resin and provided with a microchannel, wherein,

the microchannel includes a main channel having a first channel expansion section provided on a downstream side of a branch section and increasing channel resistance, and a branch channel connected to the branch section of the main channel and having a second channel expansion section provided on a downstream side of the branch section and increasing channel resistance,

wherein the inner surface of the flow path is curved at the first and second enlarged flow paths, and when the flow path width at the starting point of the first and second enlarged flow paths is x, the curvature radius of the curved inner surface of the flow path in a plan view is R, and the central angle corresponding to an arc having a curvature radius R and ending at the starting point of the first and second enlarged flow paths and the end point of the first and second enlarged flow paths is theta, the difference between the TB value as the T value of the branch flow path and the TE value as the T value of the main flow path satisfies TB-TE ≧ 5,

T＝{1/(x²r) } (theta/90) formula (1).

2. The microfluidic device of claim 1,

the branching section is provided in plurality, and the branching flow paths are connected to the branching sections in a one-to-one manner, and satisfy TB-TE ≧ 19 for each of the branching flow paths.

3. The microfluidic device according to claim 2, further comprising a connection channel connecting the second channel enlargements of the plurality of branch channels to each other.

4. The microfluidic device according to any one of claims 1 to 3, further comprising a waste liquid section connected to the first flow path expansion section.

5. The microfluidic device of any one of claims 1 to 4,

a narrowed portion that is connected to the upstream side of the second flow path expansion portion and whose flow path is narrower than the second flow path expansion portion and the rest of the branch flow path is further provided on the branch flow path.

6. The microfluidic device according to any one of claims 1 to 5, further comprising a liquid feeding mechanism provided upstream of the main channel.

Technical Field

The present invention relates to a microfluidic device having an injection-molded body of a synthetic resin.

Background

Various microfluidic devices have been proposed for use in biochemical analysis and the like. In order to transport a fluid and stop it at a predetermined portion, it is necessary to provide portions having different transport resistances in a microchannel. Patent document 1 discloses a structure provided with a channel expansion section for rapidly expanding the channel cross section of a microchannel. It is considered that the fluid can be stopped by the increase of the liquid feeding resistance in the flow path enlarging portion.

Disclosure of Invention

Technical problem to be solved by the invention

In the above-described microfluidic devices, injection-molded articles of synthetic resin are widely used to achieve miniaturization and cost reduction. In order to produce such an injection molded article of synthetic resin, it is necessary to make the inner surface of the flow path curved at an inflection point where the flow path of the flow path-enlarging portion rapidly changes. Otherwise, it is difficult to take out the injection molded body from the molding die.

However, when the vicinity of the inflection point is a curved surface, the flow easiness of the fluid varies depending on the radius of curvature of the curved surface portion. Therefore, for example, when the main flow path is provided with a flow path expansion portion and the branch flow path is provided with a flow path expansion portion, the fluid may not be reliably called the branch flow path side. That is, there is a risk that the fluid flows from the branch flow path as the weighing unit to the downstream side.

Further, even in the case where the fluid is dispensed into a plurality of branch channels, there is a risk that the fluid cannot be dispensed into each branch channel reliably.

The present invention provides a microfluidic device capable of reliably weighing a fluid in a branch channel and dispensing the fluid into a plurality of branch channels.

Means for solving the problems

A microfluidic device of the present invention includes an injection-molded body made of a synthetic resin, and is provided with a microchannel, wherein the microchannel includes a main channel and a branch channel, the main channel includes a branch portion and a first channel expansion portion provided on a downstream side of the branch portion and increasing a channel resistance, the branch channel is connected to the branch portion of the main channel, and includes a second channel expansion portion provided on the downstream side of the branch portion and increasing a channel resistance, a channel inner surface is curved in the first and second channel expansion portions, a radius of curvature of the curved channel inner surface in a plan view is R when a channel width at a start point of the first and second channel expansion portions is x, and a central angle corresponding to an arc having a radius of curvature of R with the start point of the first and second channel expansion portions and an end point of the first and second channel expansion portions as ends is θ, the difference between the TB value as the T value of the branch flow path and the TE value as the T value of the main flow path satisfies TB-TE ≧ 5,

T＝{1/(x²r) } (theta/90) formula (1).

In a specific aspect of the microfluidic device according to the present invention, a plurality of the branch portions are provided, a plurality of the branch flow paths are connected to the plurality of branch portions in a one-to-one manner, respectively, and each of the branch flow paths satisfies TB-TE ≧ 19. In this case, the fluid can be dispensed reliably into the plurality of branch channels.

In another specific aspect of the microfluidic device according to the present invention, the microfluidic device further includes a connection channel that connects the second channel enlargements of the plurality of branch channels to each other.

In another specific aspect of the microfluidic device according to the present invention, the microfluidic device further includes a waste liquid portion connected to the first flow path enlarging portion.

In another specific aspect of the microfluidic device of the present invention, a narrowed portion which is connected to the upstream side of the second flow-path expansion portion and whose flow path is narrower than the second flow-path expansion portion and the rest of the branch flow path is further provided on the branch flow path.

In another specific aspect of the microfluidic device according to the present invention, the microfluidic device further includes a liquid feeding mechanism provided upstream of the main channel.

Effects of the invention

According to the microfluidic device of the present invention, in the microfluidic device having the injection molded body, a predetermined amount of fluid can be reliably weighed in the branch flow path, and a predetermined amount of fluid can be reliably dispensed into the plurality of branch flow paths.

Drawings

Fig. 1 is a perspective view showing an external appearance of a microfluidic device according to an embodiment of the present invention.

FIG. 2 is a schematic plan view of a microchannel for explaining a microfluidic device according to an embodiment of the present invention.

Fig. 3 is a schematic plan view for explaining a flow path width x, a curvature radius R, and an angle θ.

FIG. 4 is a schematic sectional view showing a direction of enlarging a cross section of a flow path in FIG. 4.

Fig. 5 is a schematic plan view for explaining a curved portion in the flow path expansion portion in the case where the angle θ is 120 °.

Fig. 6 is a schematic plan view for explaining a curved portion in the flow path expansion portion in the case where the angle θ is 60 °.

Detailed Description

Hereinafter, the present invention will be explained by explaining specific embodiments of the present invention with reference to the accompanying drawings.

Fig. 1 is a perspective view showing an external appearance of a microfluidic device according to an embodiment of the present invention. The microfluidic device 1 has a substrate 2 made of an injection molded body of synthetic resin. A cover plate 3 is laminated on the base plate 2, and a base plate 4 is laminated on the lower surface of the base plate 2. The cover plate 3 and the base plate 4 contain an elastomer or an inorganic synthetic resin. A microchannel is provided in the substrate 2.

The microchannel is a fine channel that produces a microscopic effect when a liquid (micro liquid) is transported.

In such a microchannel, the liquid is influenced by a strong surface tension and exhibits a behavior different from that of the liquid flowing through a channel of a normal size.

The cross-sectional shape and size of the microchannel are not particularly limited as long as the above-described microscopic effects are produced. For example, when a fluid is caused to flow through a microchannel, when the microchannel has a substantially rectangular cross-sectional shape (including a square shape) in view of reducing the channel resistance by using a pump or gravity, the size of the smaller side is preferably 20 μm or more, more preferably 50 μm or more, and still more preferably 100 μm or more. From the viewpoint of miniaturization of the microfluidic device, the size of the smaller side is preferably 5mm or less, more preferably 1mm or less, and further preferably 500 μm or less. In the case where the cross-sectional shape of the microchannel is substantially circular, the diameter (short diameter in the case of an ellipse) is preferably 20 μm or more, more preferably 50 μm or more, and still more preferably 100 μm or more. From the viewpoint of miniaturization of the microfluidic device, the diameter (short diameter in the case of an ellipse) is preferably 5mm or less, more preferably 1mm or less, and further preferably 500 μm or less.

On the other hand, for example, when a fluid is caused to flow through a microchannel, in the case where the capillary phenomenon is effectively used, in the case where the cross-sectional shape of the microchannel is substantially rectangular (including square), the size of the smaller side is preferably 5 μm or more, more preferably 10 μm or more, further preferably 20 μm or more, and preferably 200 μm or less, more preferably 100 μm or less.

As shown in fig. 2, the microchannel 11 has a main channel 12. A micro pump 13 as a liquid feeding mechanism is provided on the upstream side of the main channel 12.

The main channel 12 is provided with a plurality of branching portions 12a to 12 c. The first expanded flow passage portion 12d is provided downstream of the portions where the branch portions 12a to 12c are provided. The first expanded channel portion 12d is a portion in which the channel cross section of the main channel 12 rapidly expands. The first channel expansion portion 12d determines the liquid feeding resistance of the fluid fed through the main channel 12.

The waste liquid portion 14 is connected to the first channel expansion portion 12 d.

The branch portions 12a to 12c are connected to branch flow paths 15 to 17, respectively. The branch flow paths 15 to 17 have branch flow path main body portions 15a to 17a connected to the branch portions 12a to 12 c. The post-branching flow path narrowing portions 15b to 17b are connected to downstream side end portions of the branch flow path main body portions 15a to 17 a. The second expanded flow path portions 15c to 17c are connected to downstream end portions of the post-branching flow path narrowing portions 15b to 17 b. Downstream ends of the second expanded flow passages 15c, 16c, and 17c are connected to a connection flow passage 18. Further, a bypass flow path 19 is provided to connect the first enlarged flow path portion 12d and the connection flow path 18.

The flow path cross-sections of the post-branching flow path narrowing portions 15b, 16b, 17b are narrower than the flow path cross-sections of the second flow path expanding portions 15c, 16c, 17c and the branch flow path main body portions 15a, 16a, 17a which are the remaining portions of the branch flow paths 15, 16, 17. The second expanded channel portions 15c, 16c, and 17c are portions whose channel cross-sections rapidly expand, and provide liquid feeding resistance to the fluid in the respective branch channels 15, 16, and 17.

In the feature of this embodiment, the value of T shown in the following formula (1) is set to TB — TE of 5 or more, and more preferably 19 or more. Note that the TB value is a T value in the second expanded portions 15c, 16c, and 17c of the branch channels 15, 16, and 17, and the TE value is a T value in the first expanded portion 12d of the main channel 12.

T＝{1/(x²R) } (theta/90) formula (1)

The above-described T value will be explained with reference to fig. 3. As a representative example, fig. 3 is a schematic plan view showing an enlarged portion of the branch flow passage 15 where the post-branching flow passage narrowing portion 15b and the second flow passage widening portion 15c are connected. Here, the channel width x in the formula (1) is the channel width (unit: μm) at the starting point 15c1 of the second expanded channel section 15 c.

In the second flow path expansion portion 15c, the flow path cross section gradually increases. Here, since the substrate 2 made of an injection molded body is used, it is necessary that the inner wall of the flow path is curved like the second expanded flow path portion 15c in order to perform injection molding. In the second expanded flow passage portion 15c, the radius of curvature is R (unit: μm) when the curved portion is viewed in plan. Further, θ (°) is a central angle corresponding to the arc Ra of the radius R having the start point 15c1 and the end point 15c2 as end points of the second expanded flow path portion 15 c. Therefore, in fig. 3, θ is 90 °.

In the flow path expansion portion, the flow path cross section gradually increases in the second flow path expansion portion 15C in a plan view, the flow path cross section gradually increases in the vertical direction indicated by arrows a and B in fig. 4, and the flow path cross section gradually increases in the left-right direction indicated by arrows C and D.

Note that, in fig. 3, the angle θ is 90 °. Fig. 5 and 6 are schematic plan views showing curved portions of the second expanded flow passage portion 15c when θ is 120 ° and 60 °, respectively. As shown in fig. 5, the arc Ra of the radius R has a start point 15c1 and an end point 15c2 as end points. In fig. 5, the center angle θ corresponding to the arc Ra is 120 °. In fig. 6, the center angle θ corresponding to the arc Ra is 60 °.

In the microfluidic device 1, the TB — TE is set to 5 or more, and more preferably 19 or more, in the microchannel 11, so that a predetermined amount of fluid can be weighed in the branch channels 15, 16, and 17, or a predetermined amount of fluid can be dispensed into the branch channels 15, 16, and 17 with certainty. This will be explained based on the following experimental examples.

(Experimental examples 1 to 16)

A microfluidic device 1 in which a cover plate 3 and a base plate 4 are laminated on a substrate 2 as an injection-molded body containing a cycloolefin polymer was prepared. In this microfluidic device 1, a microchannel 11 having two branch flow paths 15, 16 of various sizes is provided. Table 1 below shows design parameters of the flow path expansion section used as the first and second flow path expansion sections 12d, 15c, or 16 c. T1 to T36 in Table 1 indicate the numbers of the flow path enlarging portions.

As experimental examples 1 to 16, each microfluidic device 1 was produced as shown in table 2 below, and the second channel expansion section and the first channel expansion section were formed to have a size indicated by a T-number. The TB values and TE values are shown together in Table 2.

[ Table 1]

T number	×(μm)	R(μm)	θ(°)	T
					T1	1	0.2	60	3.333333333
T2	1	0.2	90	5
					T3	1	0.2	120	6.666666667
T4	1	0.4	60	1.666666667
					T5	1	0.4	90	2.5
T6	1	0.4	120	3.333333333
					T7	1	0.6	60	1.111111111
T8	1	0.6	90	1.666666667
					T9	1	0.6	120	2.222222222
T10	0.7	0.2	60	6.802721088
					T11	0.7	0.2	90	10.20408163
T12	0.7	0.2	120	13.60544218
					T13	0.7	0.4	60	3.401360544
T14	0.7	0.4	90	5.102040816
					T15	0.7	0.4	120	6.802721088
T16	0.7	0.6	60	2.267573696
					T17	0.7	0.6	90	3.401360544
T18	0.7	0.6	120	4.535147392
					T19	0.5	0.2	60	13.33333333
T20	0.5	0.2	90	20
					T21	0.5	0.2	120	26.66666667
T22	0.5	0.4	60	6.666666667
					T23	0.5	0.4	90	10
T24	0.5	0.4	120	13.33333333
					T25	0.5	0.6	60	4.444444444
T26	0.5	0.6	90	6.666666667
					T27	0.5	0.6	120	8.888888889
T28	0.2	0.2	60	83.33333333
					T29	0.2	0.2	90	125
T30	0.2	0.2	120	166.6666667
					T31	0.2	0.4	60	41.66666667
T32	0.2	0.4	90	62.5
					T33	0.2	0.4	120	83.33333333
T34	0.2	0.6	60	27.77777778
					T35	0.2	0.6	90	41.66666667
T36	0.2	0.6	120	55.55555556

[ Table 2]

As shown in table 2, for example, in experimental example 1, since the flow path expansion portion of T29 was present, the TB value of the branch flow path was 125. On the other hand, since the flow path expansion portion of T11 was present, the TE value in experimental example 1 was 10.2. Therefore, TB-TE was 114.8.

As described above, the microfluidic devices 1 of examples 1 to 16 having different TB-TEs were fabricated.

In the above-described microfluidic device 1, an aqueous solution having a contact angle of 90 ° was delivered using the micro pump 13. When a predetermined amount of fluid can be dispensed into the two branch channels 15 and 16, table 3 below shows a result of dispensing. X is given when a predetermined amount of fluid cannot be dispensed reliably into the plurality of branch flow paths 15, 16.

[ Table 3]

As is apparent from Table 3, if TB-TE is 19 or more, the fluid can be dispensed into the branch flow paths 15 and 16 with reliability.

(Experimental examples 17 to 32)

Next, a microfluidic device having one of the above-described branch flow paths was produced in the same manner as described above. That is, the microfluidic device 1 was fabricated in the same manner as in experimental examples 1 to 16 described above, except that only one branch flow path 15 was connected to the main flow path without the branch flow path 16. With respect to TB of this branched flow path, the microfluidic devices 1 of experimental examples 17 to 32 were produced in the same manner as in experimental examples 1 to 16, respectively. Also, an aqueous solution having a contact angle of 90 ° was transported in the same manner as in experimental examples 1 to 16 to confirm whether or not a 5 μ L amount of fluid was reliably weighed into one branch flow path. In the case where the weighing was reliably performed, a circle is given in the following table 4, and in the case where the weighing was not reliably performed, a x is given.

[ Table 4]

As is apparent from table 4, if TB — TE is 5 or more, a predetermined amount of fluid can be reliably weighed into one branch flow path.

It should be noted that the fluid that can be used is not particularly limited, and it has been confirmed that, as long as the fluid has a contact angle in the range of 70 ° to 130 °, the fluid can be reliably weighed or dispensed according to the present invention in the same manner as in the above-described experimental examples 1 to 32.

Description of the symbols

1. microfluidic device

2. substrate

3. cover plate

4. bottom plate

11. Microchannel

12. main flow path

12a to 12 c. branch

12 d. first flow path enlarging portion

13. micropump

14. waste liquid section

15 to 17. branch flow path

15a to 17 a. branch flow path main body part

15b to 17b DEG A flow path narrowing section after branching

15c to 17 c.second flow path expansion part

15c 1. start point

15c 2. end-point

18. connecting flow path

19. bypass flow path