阅读说明：本技术 一种培养基制备装置及其工作方法 (Culture medium preparation device and working method thereof ) 是由 黄艳花 蒙成 覃连红 宁平 崔忠吉 于 2019-08-26 设计创作，主要内容包括：本发明涉及实验室器材技术领域,具体讲的是一种培养基制备装置及其工作方法,装置包括锅体(1)和用于给所述锅体(1)加热的电加热板(2),锅体(1)具有液体加热腔(3)；所述培养基制备装置还包括过滤网篮(4),液体加热腔(3)内沿上下方向滑动连接有驱动块(5),过滤网篮(4)插接在所述驱动块(5)上,液体加热腔(3)的侧壁上端转动连接有用于盛装辅料的辅料盒(6)。本申请不需要在将煮沸后的马铃薯液体从锅体中倒出,之后再回锅,方便使用；本申请的装置可以实现自动将煮过之后的马铃薯和液体过滤,并且可以自动加入葡萄糖和琼脂,实现了马铃薯培养基制备一体化,方便使用。(The invention relates to the technical field of laboratory equipment, in particular to a culture medium preparation device and a working method thereof, wherein the culture medium preparation device comprises a pot body (1) and an electric heating plate (2) for heating the pot body (1), wherein the pot body (1) is provided with a liquid heating cavity (3); culture medium preparation facilities still includes filtration basket (4), has drive block (5) along upper and lower direction sliding connection in liquid heating chamber (3), and filtration basket (4) are pegged graft on drive block (5), the lateral wall upper end rotation in liquid heating chamber (3) is connected with auxiliary material box (6) that are used for the splendid attire auxiliary material. The boiled potato liquid does not need to be poured out of the pot body and then returned to the pot, so that the use is convenient; the device of this application can realize that automatic potato and the liquid after will boiling filter to can add glucose and agar automatically, realized potato culture medium preparation integration, facilitate the use.)

1. A culture medium preparation device comprises a pot body (1) and an electric heating plate (2) used for heating the pot body (1), wherein the pot body (1) is fixed on the electric heating plate (2), and the pot body (1) is provided with a liquid heating cavity (3); the culture medium preparation device is characterized by further comprising a filter basket (4), wherein the filter basket (4) is located in the liquid heating cavity (3), a driving block (5) is connected in the liquid heating cavity (3) in a sliding mode along the vertical direction, the filter basket (4) is connected onto the driving block (5) in an inserting mode, a driving assembly used for driving the driving block (5) to slide vertically is arranged on the outer side wall of the pot body (1), and the driving block (5) is connected with the driving assembly in a driving mode; the auxiliary material box (6) that are used for the splendid attire auxiliary material are connected with in the rotation of the lateral wall upper end of liquid heating chamber (3), be fixed with on the lateral wall of the pot body (1) and be used for the drive auxiliary material box (6) pivoted drive unit.

2. A medium preparation device according to claim 1, wherein a slide rail (7) is fixedly connected to the inside of the liquid heating chamber (3), and the driving block (5) is slidably connected to the slide rail (7).

3. The culture medium preparation device according to claim 2, wherein the driving assembly comprises a first motor (8), a pulley (9), a driving rope (10) and a winding drum (11), the first motor (8) is fixed on the side wall of the pot body (1), the winding drum (11) is fixedly connected with a rotating shaft of the first motor (8), the driving rope (10) is wound on the winding drum (11), the pulley (9) is rotatably connected to the upper end of the side wall of the pot body (1), the middle part of the driving rope (10) is erected on the pulley (9), and the end part of the driving rope (10) is fixedly connected with the driving block (5).

4. A device for the preparation of a culture medium according to claim 2, wherein the driving block (5) is provided with a socket (13), the filter basket (4) is fixedly connected with an insert rod (14), and the end of the insert rod (14) is inserted into the socket (13).

5. A medium preparation device according to claim 4, wherein a screw (15) is threadedly connected to said driving block (5), and the top of said screw (15) abuts said plunger (14).

6. A culture medium preparation device according to claim 1, wherein a rotating rod (16) is rotatably connected to the side wall of the pot body (1), one end of the rotating rod (16) is fixedly connected with the auxiliary material box (6), the rotating rod (16) is arranged to penetrate through the side wall of the pot body (1), and the other end of the rotating rod (16) is fixedly connected with the driving end of the driving unit.

7. A medium preparation device according to claim 6, wherein said drive unit is a second motor (12).

8. A medium preparation device according to claim 1, further comprising a pot lid (17), wherein said pot lid (17) is attached to said pot body (1).

9. A working method of a culture medium preparation device is characterized by comprising the following steps:

1) putting the cut potato blocks into a filter basket (4), and adding water into the liquid heating cavity (3) to immerse the potato blocks in the water;

2) placing the weighed glucose and agar in an auxiliary material box (6);

3) starting the stirrer (19), starting the electric heating plate (2), heating the pot body (1), and boiling water in the liquid heating cavity (3) for 25-35 min to obtain potato liquid;

4) the driving assembly drives the driving block (5) to ascend, the driving block (5) drives the filtering basket (4) to ascend, the filtering basket (4) leaves the liquid level, and the potatoes are left in the filtering basket (4) to finish filtering;

5) opening an electric control water outlet valve, and injecting water into the liquid heating cavity (3) to enable the potato liquid to reach a preset volume;

6) the driving unit drives the auxiliary material box (6) to rotate, and glucose and agar in the auxiliary material box (6) are poured into the liquid heating cavity (3) and are mixed with the potato liquid;

7) the stirrer (19) continuously stirs, and the liquid in the liquid heating cavity (3) is kept at the temperature of 50-70 ℃ to form the potato culture medium.

Technical Field

The invention relates to the technical field of laboratory equipment, in particular to a culture medium preparation device and a working method thereof.

Background

PDA culture medium is short for potato glucose agar culture medium, and its preparation method is: 1. peeling potato, weighing the potato according to the formula of the culture medium, cutting the potato into small pieces, putting the small pieces into a cooking pot with cooking function, and adding water to boil for 20-30 min. 2. And (3) taking gauze and a measuring cup, filtering by using the gauze when the gauze and the measuring cup are hot, removing filter residues, and keeping the filtrate in the measuring cup. 3. Pouring the filtrate into the pan again, weighing glucose and agar, heating with slow fire and stirring to dissolve completely. 4. The prepared culture medium is subpackaged in test tubes or triangular flasks, and a triangular funnel is generally adopted for subpackaging.

The boiling pot adopted by the method has single function, the potato liquid needs to be filtered by another gauze and a measuring cup after being boiled, dregs are removed and poured out, the obtained filtered liquid is poured back into the pot and then mixed with glucose and agar for re-heating and dissolving, and the operation is troublesome.

Disclosure of Invention

The present invention is directed to solving at least one of the above problems.

The invention aims to solve the technical problem of at least providing a culture medium preparation device which is convenient for preparing a potato culture medium.

In order to solve the problems, the culture medium preparation device comprises a pot body and an electric heating plate for heating the pot body, wherein the pot body is fixed on the electric heating plate and is provided with a liquid heating cavity; the culture medium preparation device further comprises a filter basket, the filter basket is located in the liquid heating cavity, a driving block is connected in the liquid heating cavity in a sliding mode along the vertical direction, the filter basket is inserted into the driving block, a driving assembly used for driving the driving block to slide vertically is arranged on the outer side wall of the pot body, and the driving block is in driving connection with the driving assembly; the lateral wall upper end of liquid heating chamber rotates and is connected with the auxiliary material box that is used for the splendid attire auxiliary material, be fixed with on the lateral wall of the pot body and be used for the drive auxiliary material box pivoted drive unit.

After adopting above-mentioned scheme, have at least following advantage: when preparing the potato culture medium, firstly adding proper water into the liquid heating cavity, putting the cut potatoes into the filter basket, and positioning the bottom of the filter basket in the liquid heating cavity, namely positioning the potatoes in the liquid; secondly, placing the weighed glucose and agar in an auxiliary material box, wherein the auxiliary material box is higher than the liquid level in the liquid heating cavity; the electric heating board work, boil it for a period of time for example 30min, later drive assembly drive the drive block rises, and the drive block drives the filtration basket and rises, leaves the liquid level, and the potato is stayed in the filtration basket, can accomplish the filtration this moment, and later drive unit drive auxiliary material box rotates, and glucose and agar in the auxiliary material box pour into liquid heating intracavity, reheat a period can prepare into potato culture medium. Therefore, the device of this application need not pour out in the potato liquid after will boiling from the pot body, later return the pot, facilitates the use, and the device of this application can realize that automatic potato and the liquid after will boiling filter to can add glucose and agar automatically, realized potato culture medium preparation integration, facilitate the use.

In addition, the application also discloses a working method of the culture medium preparation device, which comprises the following steps:

1) putting the cut potato blocks into a filter basket, and adding water into a liquid heating cavity to immerse the potato blocks in the water;

2) placing the weighed glucose and agar in an auxiliary material box;

3) starting the stirrer, starting the electric heating plate, heating the pot body, and boiling water in the liquid heating cavity for 25-35 min to obtain potato liquid;

4) the driving assembly drives the driving block to ascend, the driving block drives the filtering basket to ascend, the filtering basket leaves the liquid level, and the potatoes are left in the filtering basket to finish filtering;

5) opening an electric control water outlet valve, and injecting water into the liquid heating cavity to enable the potato liquid to reach a preset volume;

6) the driving unit drives the auxiliary material box to rotate, and glucose and agar in the auxiliary material box are poured into the liquid heating cavity and mixed with the potato liquid;

7) and (3) continuously stirring by using a stirrer, and preserving the heat of the liquid in the liquid heating cavity at 50-70 ℃ to form the potato culture medium.

By adopting the method, the automatic filtration can be realized, the glucose and the agar are automatically poured after the filtration is finished, the stirring is automatically carried out, and the automation of the preparation of the potato culture medium is realized.

Drawings

FIG. 1 is a schematic view of a medium preparing apparatus according to the present application.

FIG. 2 is a schematic view of another perspective structure of a medium preparation device according to the present application.

FIG. 3 is a schematic diagram of a medium preparation apparatus according to the present application.

Wherein:

1. a pan body; 2. an electrical heating plate; 3. a liquid heating chamber; 4. a filter basket; 5. a drive block; 6. an auxiliary material box; 7. a slide rail; 8. a first motor; 9. a pulley; 10. a drive rope; 11. a hoisting drum; 12. a second motor; 13. a jack; 14. inserting a rod; 15. a screw; 16. rotating the rod; 17. a pot cover; 18. split charging of the pump; 19. a stirrer.

Detailed Description

The inventive concepts of the present disclosure will be described hereinafter using terms commonly employed by those skilled in the art to convey the substance of their work to others skilled in the art. These inventive concepts may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. These embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of their inclusion to those skilled in the art. It should also be noted that these embodiments are not mutually exclusive. A component, step, or element from one embodiment may be assumed to be present or used in another embodiment. The particular embodiments shown and described may be substituted for a wide variety of alternate and/or equivalent implementations without departing from the scope of the embodiments of the present disclosure. This application is intended to cover any adaptations or variations of the embodiments discussed herein. It will be apparent to those skilled in the art that alternative embodiments may be practiced using only some of the described aspects. Specific numbers, materials, and configurations are set forth in the examples for the purpose of illustration, however, alternative examples may be practiced by those skilled in the art without these specific details. In other instances, well-known features may be omitted or simplified in order not to obscure the illustrative embodiments.

Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "provided", "mounted", "connected" and "connected" are to be interpreted broadly, e.g., as being either fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. "fixing" may be welding or fixing by screws, bolts, etc., and it is obvious to those skilled in the art that the above terms have specific meanings in the present invention.

Referring to fig. 1-3, in one embodiment, a medium preparing apparatus includes a pot body 1 and an electric heating plate 2 for heating the pot body 1, the pot body 1 is fixed on the electric heating plate 2, the pot body 1 has a liquid heating chamber 3; the culture medium preparation device further comprises a filter basket 4, the filter basket 4 is located in the liquid heating cavity 3, a driving block 5 is connected in the liquid heating cavity 3 in a sliding mode along the vertical direction, the filter basket 4 is connected to the driving block 5 in an inserting mode, a driving assembly used for driving the driving block 5 to slide vertically is arranged on the outer side wall of the pot body 1, and the driving block 5 is connected with the driving assembly in a driving mode; the lateral wall upper end of liquid heating chamber 3 rotates and is connected with auxiliary material box 6 that is used for the splendid attire auxiliary material, be fixed with on the lateral wall of the pot body 1 and be used for the drive 6 pivoted drive units of auxiliary material box.

The liquid heating cavity 3 is internally and fixedly connected with a slide rail 7, and the driving block 5 is in sliding connection with the slide rail 7. Thus, the driving block 5 is matched with the slide rail 7, so that the driving block 5 can slide up and down smoothly. The slide rail 7 is provided in the up-down direction. The number of the slide rails 7 is two.

The driving assembly comprises a first motor 8, a pulley 9, a driving rope 10 and a winding drum 11, the first motor 8 is fixed on the side wall of the pot body 1, the winding drum 11 is fixedly connected with a rotating shaft of the first motor 8, the driving rope 10 is wound on the winding drum 11, the pulley 9 is rotatably connected to the upper end of the side wall of the pot body 1, the middle of the driving rope 10 is erected on the pulley 9, and the end part of the driving rope 10 is fixedly connected with the driving block 5. The winding drum 11 is used for winding the driving rope 10, when the first motor 8 rotates, the winding drum 11 rotates, and when the winding drum 11 enables the driving rope 10 to be wound and contracted, the driving rope 10 is shortened, so that the driving block 5 is lifted upwards, and the filtering basket 4 is driven to ascend; when the winding drum 11 curls the drive rope 10 out, the drive rope 10 lengthens, thereby moving the drive block 5 downward by gravity.

The driving block 5 is provided with a jack 13, the filtering net basket 4 is fixedly connected with an inserted link 14, and the end part of the inserted link 14 is inserted into the jack 13. The matching of the inserted bar 14 and the inserting hole 13 can ensure that the filter screen basket 4 can be detachably fixed on the driving block 5, the filter screen baskets 4 with different filter densities can be replaced, and the cross section of the inserting hole 13 is adaptive to the cross section of the inserted bar 14 and can be quadrilateral, pentagonal, hexagonal and the like.

The driving block 5 is connected with a screw 15 in a threaded manner, and the top of the screw 15 is abutted to the inserted rod 14. In this way, the presence of the screw 15 can further fix the plunger 14. When the screws are loosened, the insertion rod 14 is convenient to pull out the insertion hole 13, and replacement is convenient.

The utility model discloses a pot body, including pot body 1, the lateral wall of pot body 1 is last to rotate and to be connected with dwang 16, the one end of dwang 16 with 6 fixed connection of auxiliary material box, dwang 16 passes the lateral wall of the pot body 1 sets up, the other end of dwang 16 with drive unit's drive end fixed connection. The auxiliary material box 6 is provided with an opening, glucose and agar can be placed from the opening, in a normal state, the opening of the auxiliary material box 6 faces upwards, when the driving unit rotates, the rotating rod 16 is driven to rotate, the rotating rod 16 drives the auxiliary material box 6 to rotate, specifically, the auxiliary material box rotates by 180 degrees, the opening of the auxiliary material box 6 faces downwards, the glucose and the agar in the auxiliary material box 6 are poured out from the auxiliary material box 6 to enter the liquid heating cavity 3 of the pot body 1 to be mixed with potato liquid, and a culture medium is formed.

The drive unit is a second motor 12.

The culture medium preparation device also comprises a pot cover 17, and the pot cover 17 covers the pot body 1. The pot cover 17 can prevent contaminants such as dust from entering the pot body 1.

Still be equipped with on the lateral wall of the pot body 1 and be used for the partial shipment pump 18 that the liquid in the pot body 1 was taken out, the feed liquor end of partial shipment pump 18 pass through the pipeline with liquid heating chamber 3 intercommunication. The dispensing pump 18 may be a peristaltic pump or a manual pump, which is well known in the art and will not be described in detail herein.

The bottom of the liquid heating chamber 3 is provided with a stirrer 19.

The side wall of the pot body 1 can be also provided with a liquid level meter, so that the liquid position and the liquid volume in the pot body 1 can be conveniently observed.

The electric heating plate 2 may be an induction cooker or a resistance wire heating furnace, etc.

The culture medium preparation device further comprises a controller and a display, wherein the controller and the display are fixed on the side wall of the pot body 1, and the controller is used for controlling the first motor 8, the second motor 12 and the stirrer 19; the first motor 8 and the second motor 12 are electrically connected to the controller, and the first motor 8 and the second motor 12 may be stepping motors. The controller is also used to control the switching of the electric heating plate 2 and the switching of the stirrer 19.

Preferably, the culture medium preparation device further comprises a water source, an outlet of the water source is located above the liquid heating cavity 3, an electric control water outlet valve is arranged at an outlet of the water source, the electric control water outlet valve is electrically connected with the controller, and the controller can control the on-off of the electric control water outlet valve, so that the automatic water inlet function can be realized. The water source may specifically be a tap water source. The electric control water outlet valve is an electric control water faucet which is positioned above the liquid heating cavity 3.

Of course, the water source and the liquid heating chamber 3 may be connected in such a manner that the water source is communicated with the upper side of the side wall of the liquid heating chamber 3 through a pipeline, and the pipeline is provided with an electrically controlled water outlet valve; the electric control water outlet valve is electrically connected with the controller, and the controller can control the opening and closing of the electric control water outlet valve.

Preferably, the culture medium preparation device further comprises a temperature detector for detecting the temperature of the liquid in the liquid heating cavity 3, the temperature detector is electrically connected with the controller, and the controller can control the switch of the electric heating plate 2 according to a signal fed back by the temperature detector so as to adjust the temperature of the liquid in the liquid heating cavity 3.

Preferably, the liquid level meter is a liquid level sensor, the liquid level sensor is electrically connected with the controller, and the controller can acquire data signals of the liquid level sensor in real time.

The display and the controller are electrically connected for displaying temperature information in the liquid heating chamber 3, liquid level information in the liquid heating chamber 3 and the like.

The culture medium preparation device also comprises a prompting buzzer, and the prompting buzzer is electrically connected with the controller and is controlled by the controller.

The working method of the culture medium preparation device comprises the following steps:

1) putting the cut potato blocks into a filter basket 4, and adding water into the liquid heating cavity 3 to immerse the potato blocks in the water; the amount of water added can be specifically 90% of the total volume of the potato culture medium to be prepared;

2) placing the weighed glucose and agar in the auxiliary material box 6, wherein the auxiliary material box 6 is higher than the liquid level in the liquid heating cavity 3;

3) starting the stirrer 19, starting the electric heating plate 2, heating the pot body 1, and boiling water in the liquid heating cavity 3 for 25-35 min to obtain potato liquid; specifically, the water can be boiled with strong fire, the electric heating plate 2 is automatically adjusted to small fire after the water is boiled, and the water is boiled for 25 or 30 or 35 min;

4) the driving assembly drives the driving block 5 to ascend, specifically, when the controller controls the first motor 8 to rotate, the winding drum 11 rotates, when the winding drum 11 enables the driving rope 10 to curl and contract, the driving rope 10 is shortened, so that the driving block 5 ascends, the driving block 5 drives the filter screen basket 4 to ascend, the filter screen basket 4 leaves the liquid level, and the potatoes are left in the filter screen basket 4 to finish filtering;

5) opening an electric control water outlet valve, and injecting water into the liquid heating cavity 3 to enable the potato liquid to reach a preset volume; the controller has preset the liquid level volume of the liquid heating cavity 3, namely the total volume of the potato culture medium, the liquid level sensor detects the liquid level information in the liquid heating cavity 3 in real time and feeds the liquid level information back to the controller, when the liquid level is lower than the set liquid level volume of the liquid heating cavity 3, the controller controls the electric control water outlet valve to be opened, and when the liquid level reaches the set liquid level volume of the liquid heating cavity 3, the controller controls the electric control water outlet valve to be closed, so that the constant volume operation of the culture medium is realized.

6) The driving unit drives the auxiliary material box 6 to rotate, and the glucose and the agar in the auxiliary material box 6 are poured into the liquid heating cavity 3 and mixed with the potato liquid; specifically, the controller controls the second motor 12 to rotate 180 degrees, and the second motor 12 drives the auxiliary material box 6 to rotate;

7) continuously stirring by the stirrer 19, and keeping the temperature of the liquid in the liquid heating cavity 3 at 50-70 ℃ to form a potato culture medium; and prompting the buzzer to alarm and prompting the preparation completion. The heat preservation time can be 10-20 min.

8) The operator operates the dispensing pump 18 to dispense the prepared potato medium.

Therefore, the device of this application need not pour out in the potato liquid after will boiling from the pot body, later return the pot, facilitates the use, and the device of this application can realize that automatic potato and the liquid after will boiling filter to can add glucose and agar automatically, realized potato culture medium preparation integration, facilitate the use. After having the controller and loading the procedure on the controller, the device of this application can realize automatic filtration, pours into glucose and agar automatically after filtering the completion, and automatic stirring realizes the automation of potato culture medium preparation. When preparing the culture medium, workers can be relieved to do other work.

Of course, the apparatus of the present application is not limited to making potato medium.

The above description is only a preferred embodiment of the present invention, and it should not be understood that the scope of the present invention is limited thereby, and it should be understood by those skilled in the art that various other modifications and equivalent arrangements can be made by applying the technical solutions and concepts of the present invention within the scope of the present invention as defined in the appended claims.