阅读说明：本技术 咪康唑在制备用于防治植物病原菌的杀菌剂中的应用 (Application of miconazole in preparation of bactericide for preventing and treating phytopathogen ) 是由 黄金光 秦庆连 赵彦翔 孙晓梅 张淑颖 于 2019-09-12 设计创作，主要内容包括：本发明公开了咪康唑在制备用于防治植物病原菌的杀菌剂中的应用。本发明通过室内药剂敏感性测定,证明了咪康唑对重要粮食作物主要病害包括引起小麦赤霉病的禾谷镰孢菌、引起纹枯病的禾谷丝核菌及引起玉米叶斑病的玉米弯孢菌具有良好的抑制活性；而且对引起苹果轮纹病的轮纹病菌、引起苹果腐烂病的腐烂病菌、引起果蔬主要病害灰霉病的灰霉病菌以及引起果蔬叶斑病的细极链格孢菌均具有良好的抑制活性；而且对农业生产上极难防治的土传病害譬如引起枯萎病的尖孢镰刀菌也有较好的抑制活性,其抑菌活性优于传统杀菌剂多菌灵、戊唑醇。本发明提出咪康唑应用于农业生产重要植物病害的防控,市场应用前景广阔。(The invention discloses an application of miconazole in preparing a bactericide for preventing and treating plant pathogenic bacteria. According to the invention, through indoor reagent sensitivity determination, the miconazole is proved to have good inhibitory activity on main diseases of important grain crops, including fusarium graminearum causing wheat scab, rhizoctonia cerealis causing sheath blight and curvularia zeae causing corn leaf spot; but also has good inhibitory activity on ring spot pathogen causing ring spot of apple, rot pathogen causing apple rot, gray mold pathogen causing gray mold of main diseases of fruits and vegetables and alternaria tenuissima causing leaf spot of fruits and vegetables; but also has better inhibitory activity to soil-borne diseases which are extremely difficult to be controlled in agricultural production, such as fusarium oxysporum which causes blight, and the bacteriostatic activity of the compound is superior to that of the traditional bactericides carbendazim and tebuconazole. The invention provides the application of miconazole in the prevention and control of important plant diseases in agricultural production, and the application prospect in the market is wide.)

1. The application of miconazole in preparing bactericide for preventing and treating plant pathogenic bacteria.

2. The use of miconazole according to claim 1 in the preparation of a fungicide for controlling phytopathogens: the plant pathogenic bacteria are fusarium graminearum, curvularia zeae, ring rot of apple, canker of apple, alternaria tenuis, fusarium oxysporum, rhizoctonia cerealis and botrytis cinerea.

3. Use of miconazole according to claim 1 or 2 in the preparation of a fungicide for controlling phytopathogens: the using concentration of the miconazole in the bactericide is 0.1mg/L-2 mg/L.

4. Use of miconazole as defined in claim 3 in the preparation of a fungicide for controlling phytopathogens: the sterilization inhibition rate of the miconazole on the fusarium graminearum is 32-61%.

5. Use of miconazole as defined in claim 3 in the preparation of a fungicide for controlling phytopathogens: the sterilization inhibition rate of the miconazole on the Curvularia zeae is 39-83%.

6. Use of miconazole as defined in claim 3 in the preparation of a fungicide for controlling phytopathogens: the sterilization inhibition rate of the miconazole on the ring rot of apple is 60-100%.

7. Use of miconazole as defined in claim 3 in the preparation of a fungicide for controlling phytopathogens: the using concentration of the miconazole to the apple canker is 0.1mg/L-0.5mg/L, and the inhibition rate is 42% -100%.

8. Use of miconazole as defined in claim 3 in the preparation of a fungicide for controlling phytopathogens: the inhibition rate of the miconazole on alternaria tenuissima is 23% -56%, and the inhibition rate of the miconazole on fusarium oxysporum is 25% -49%.

9. Use of miconazole as defined in claim 3 in the preparation of a fungicide for controlling phytopathogens: the inhibition rate of miconazole on rhizoctonia cerealis is 50% -76%, and the inhibition rate of miconazole on botrytis cinerea is 49% -61%.

10. Use of miconazole as defined in claim 3 in the preparation of a fungicide for controlling phytopathogens: the plant is selected from wheat, oat, rice, corn, soybean, cotton, sweet potato, apple, melon, solanaceae, banana, and cotton.

Technical Field

The invention belongs to the technical field of plant protection, and particularly relates to application of miconazole in preparation of a bactericide for preventing and treating plant pathogenic bacteria.

Background

The united nations Food and Agriculture Organization (FAO) estimates that the influence of the yield of crops on trees is difficult to estimate due to about 40 percent loss caused by the damage of pests and weeds (4400-5500 billion yuan). The loss of work recovery of scientists and various technical popularization personnel engaged in pest and disease research worldwide reaches 3000-. Chemical control has long been the primary means of controlling plant diseases. The long-term use of a large amount of chemical pesticides not only causes resistance problems, but also causes medicament residues, kills non-target organisms, pollutes the ecological environment and finally causes harm to human bodies through biological enrichment. In order to solve the problems, the design and screening of green small molecular compounds by using the three-dimensional structure of the plant pathogenic microorganism target protein becomes a research hotspot.

In recent years, environmental safety issues have attracted attention from people in all fields, and environmental pollution, residue, drug resistance and the like have generated great interest in green small molecule compounds. Therefore, sustainable development of agriculture needs a new and safe measure for controlling plant diseases. Under such a large environment, development and effective utilization of a novel, environmentally friendly bactericide are urgently required.

Disclosure of Invention

The invention aims to provide application of miconazole in preparation of bactericide for preventing and treating phytopathogen. The method uses the three-dimensional structure of the target protein to screen the green small molecular compound miconazole for preventing and controlling plant diseases, the bacteriostatic activity of the miconazole is superior to that of the traditional bactericides carbendazim and tebuconazole, and the method is environment-friendly and has wide market application prospect.

In order to realize the purpose of the invention, the invention adopts the following technical scheme to realize:

the invention provides application of miconazole in preparing a bactericide for preventing and treating plant pathogenic bacteria.

Further: the plant pathogenic bacteria are fusarium graminearum, curvularia zeae, ring rot of apple, canker of apple, alternaria tenuis, fusarium oxysporum, rhizoctonia cerealis and botrytis cinerea.

Further: the using concentration of the miconazole in the bactericide is 0.1mg/L-2 mg/L.

Further: the sterilization inhibition rate of the miconazole on the fusarium graminearum is 32-61%.

Further: the sterilization inhibition rate of the miconazole on the Curvularia zeae is 39-83%.

Further: the sterilization inhibition rate of the miconazole on the ring rot of apple is 60-100%.

Further: the using concentration of the miconazole to the apple canker is 0.1mg/L-0.5mg/L, and the inhibition rate is 42% -100%.

Further: the inhibition rate of the miconazole on alternaria tenuissima is 23% -56%, and the inhibition rate of the miconazole on fusarium oxysporum is 25% -49%.

Further: the inhibition rate of miconazole on rhizoctonia cerealis is 50% -76%, and the inhibition rate of miconazole on botrytis cinerea is 49% -61%.

Further: the plant is selected from wheat, oat, rice, corn, soybean, cotton, sweet potato, apple, melon, solanaceae, banana, and cotton.

Compared with the prior art, the invention has the advantages and the technical effects that: the invention synthesizes the small molecular compound miconazole by screening, and the invention proves that the miconazole has good inhibitory activity to the main diseases of important grain crops, including fusarium graminearum causing wheat scab, rhizoctonia cerealis causing sheath blight and corn curvularia causing corn leaf spot, through indoor medicament sensitivity determination; but also has good inhibitory activity to ring rot pathogen causing ring rot of apple, rot pathogen causing rot of apple, gray mold pathogen causing gray mold of main disease of fruit and vegetable and alternaria tenuissima causing leaf spot of fruit and vegetable; but also has better inhibitory activity to soil-borne diseases which are extremely difficult to be controlled in agricultural production, such as fusarium oxysporum which causes blight, and the bacteriostatic activity of the compound is superior to that of the traditional bactericides carbendazim and tebuconazole.

The green small molecular compound miconazole screened by the three-dimensional structure of the target protein is applied to the prevention and control of plant diseases, can effectively solve the problem of poor prevention effect caused by the resistance of plant pathogenic fungi such as fusarium graminearum and ring rot to existing bactericides, is environment-friendly and pollution-free, meets the drug residue toxicity standard of the world health organization, and has wide market application prospect.

Drawings

FIG. 1 is a graph showing the inhibitory effect of miconazole on the growth of Fusarium graminearum hyphae in different concentration gradients and the traditional medicaments carbendazim and tebuconazole.

FIG. 2 is a graph showing the inhibitory effect of miconazole on mycelial growth of Curvularia lunata on the conventional agents carbendazim and tebuconazole with different concentration gradients.

FIG. 3 is a graph showing the inhibitory effect of miconazole on the growth of apple ring rot germ hypha in different concentration gradients and the traditional medicaments carbendazim and tebuconazole.

FIG. 4 is a graph showing the inhibitory effect of miconazole on mycelial growth of apple rot pathogen with different concentration gradients and the traditional agents carbendazim and tebuconazole.

FIG. 5 is a graph showing the inhibitory effect of miconazole on the growth of alternaria tenuis by different concentration gradients and conventional agents carbendazim and tebuconazole.

FIG. 6 is a graph showing the effect of miconazole in different concentration gradients and the effect of conventional carbendazim and tebuconazole in inhibiting the growth of fusarium oxysporum hyphae.

FIG. 7 is a graph showing the inhibitory effect of miconazole on the growth of sclerotinia gramineara hyphae by different concentration gradients and the traditional medicaments carbendazim and tebuconazole.

FIG. 8 is a graph showing the inhibitory effect of miconazole on the mycelial growth of Botrytis cinerea with different concentration gradients and the traditional agents carbendazim and tebuconazole.

Wherein CK is a negative control; 0.1, 0.3, 0.5, 1.0 and 2.0 represent miconazole with different concentration gradients, and the unit is mg/l; DJL0.6 and WZC0.125 are used as positive control, namely, carbendazim 0.6mg/1 and tebuconazole 0.125 mg/1.

Detailed Description

The technical solution of the present invention is further described in detail with reference to the accompanying drawings and examples.