阅读说明：本技术 一种杀菌组合物及其应用、杀菌剂 (Bactericidal composition, application thereof and bactericide ) 是由 苏正川 王敏 熊仁科 吴红波 张俊 左建英 罗小艳 于 2019-11-08 设计创作，主要内容包括：本发明涉及农药技术领域,特别涉及一种杀菌组合物及其应用、杀菌剂。该杀菌组合物由解淀粉芽孢杆菌与异菌脲组成。室内毒力测定试验和大田实验表明,解淀粉芽孢杆菌与异菌脲按照一定比例复配后具有明显的增效作用,对番茄灰霉病、马铃薯早疫病、油菜菌核病和茶炭疽病有很好的防治效果,可用于各类作物的灰霉病、早疫病、菌核病和炭疽病的防治,特别是在农药减量增效中具有良好的作用。(The invention relates to the technical field of pesticides, and particularly relates to a bactericidal composition, application thereof and a bactericide. The bactericidal composition consists of bacillus amyloliquefaciens and iprodione. Indoor toxicity test experiments and field experiments show that after being compounded according to a certain proportion, the bacillus amyloliquefaciens and the iprodione have obvious synergistic effect, have good control effect on tomato gray mold, potato early blight, rape sclerotinia rot and tea anthracnose, can be used for controlling gray mold, early blight, sclerotinia rot and anthracnose of various crops, and particularly have good effect in pesticide decrement and synergism.)

1. A bactericidal composition characterized by consisting of Bacillus amyloliquefaciens and iprodione.

2. The bactericidal composition of claim 1, wherein the mass ratio of the bacillus amyloliquefaciens to the iprodione is (1-50) to (1-50).

3. The bactericidal composition of claim 1, wherein the mass ratio of the bacillus amyloliquefaciens to the iprodione is (1-9) to (1-9).

4. The bactericidal composition of claim 1, wherein the number of viable spores of the bacillus amyloliquefaciens ranges from 1 to 1000 hundred million/gram.

5. The use of the fungicidal composition of any one of claims 1 to 4 for the control of fungal diseases in crops.

6. The use of claim 5, wherein the fungal diseases include gray mold, early blight, sclerotinia, and anthracnose, and the crops include fruit crops, vegetable crops, legume crops, oil crops, flower crops, and medicinal crops.

7. A bactericide, which is characterized by comprising the bactericidal composition as defined in any one of claims 1 to 4 and an agriculturally and pharmaceutically acceptable auxiliary material.

8. The bactericide as claimed in claim 7, wherein the mass percentage of the bactericidal composition in the bactericide is 7-70%.

9. The bactericide as claimed in claim 7, wherein the bactericide is in the form of wettable powder, water dispersible granule, suspension or suspoemulsion.

10. The fungicide according to claim 7, wherein the agriculturally and pharmaceutically acceptable auxiliary materials are one or more of wetting agent, dispersing agent, penetrating agent, antifreezing agent, thickening agent, synergist and filler.

Technical Field

The invention relates to the technical field of pesticides, and particularly relates to a bactericidal composition, application thereof and a bactericide.

Background

Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) is one of plant root growth promoting bacteria, and has the functions of improving crop yield and inhibiting the growth of plant pathogenic bacteria and nematodes. It can colonize around plant root system, produce several antibacterial substances of surfactant, bacillomycin D, pansin, iturin and cyclic bacteriocin, etc. and can be used for inducing plant system resistance, and has strong inhibiting effect for pathogenic bacteria of botrytis cinerea, fusarium, sclerotinia sclerotiorum, myceliophthora scabiosus and lawsonia etc.. Can be used for preventing and treating diseases of various crops (such as tobacco, lettuce, citrus, potato and cucumber), and has good preventing and treating effect. Meanwhile, the bacillus amyloliquefaciens can produce a plurality of physiological active substances and amino acid substances such as gibberellin, indoleacetic acid, cytokinin, abscisic acid and the like. The bacillus amyloliquefaciens regulates and controls the expression of related genes of plants and regulates community structures of other organisms in the rhizosphere soil through the synthesis of the substances, thereby achieving the effects of activating soil nutrients, improving the physical and chemical properties of the soil, increasing the soil fertility, antagonizing pathogens, reducing plant diseases, improving the disease resistance, promoting the growth and development of crops, increasing the yield of the crops and improving the quality.

The iprodione belongs to dicarboximide bactericides, is a high-efficiency broad-spectrum contact-killing type protective bactericide, has a certain treatment effect, can also absorb and absorb systemic action through roots, and can effectively prevent and control fungi with resistance to benzimidazole systemic bactericides. The main prevention and treatment objects are diseases caused by botrytis, alternaria, sclerotinia and the like, such as gray mold, early blight, black spot, sclerotinia and the like.

In the actual process of agricultural production, the most easily generated problem for preventing and controlling diseases is the generation of disease resistance. The compounding of different kinds of components is a common method for preventing and treating resistant diseases. Different components are compounded, and whether a certain compound has synergism, addition or antagonism is judged according to the actual application effect. In most cases, the compounding effect of the pesticide is additive effect, the compounding with real synergistic effect is less, and especially the compounding with very obvious synergistic effect and very high synergistic ratio is less. Through the research of the inventor, the inventor finds that the mutual compounding of the amylolytic bacillus and the active ingredients has good synergistic effect in a certain range, and relevant compounding of the amylolytic bacillus and the active ingredients is not reported at home and abroad at present.

Disclosure of Invention

In view of the above, the present invention provides a bactericidal composition, and applications and preparations thereof. The bactericidal composition has a synergistic control effect on gray mold, early blight, sclerotinia and anthracnose, prolongs the using time of the pesticide, reduces the cost, and is environment-friendly and safe.

In order to achieve the above purpose, the invention provides the following technical scheme:

the invention provides a bactericidal composition which consists of bacillus amyloliquefaciens and iprodione.

The synergistic coefficient SR of the bacillus amyloliquefaciens and the iprodione in different proportions is calculated according to Wadley (1945), the antagonism is achieved when the SR is less than 0.5, the addition effect is achieved when the SR is more than or equal to 0.5 and less than or equal to 1.5, and the synergism is achieved when the SR is more than 1.5. The result shows that the bacillus amyloliquefaciens and the iprodione are compounded according to the proportion of (1-9) to (1-9), the synergistic effect is obvious, the use amount of the chemical bactericide is greatly reduced, the potential drug resistance risk of the existing pathogenic bacteria to the iprodione is reduced, the effect of the bacillus amyloliquefaciens on effectively controlling gray mold, early blight, sclerotinia sclerotiorum and anthracnose is also exerted, the defect of slow control effect of the bacillus amyloliquefaciens is overcome, and the advantages of the biocontrol sclerotium chemical bactericide are simultaneously integrated.

In some embodiments, the mass ratio of Bacillus amyloliquefaciens to iprodione is (1-50): (1-50). Preferably, the mass ratio of the bacillus amyloliquefaciens to the iprodione is (1-9) to (1-9). In some embodiments, the mass ratio of bacillus amyloliquefaciens to iprodione is 1:9, 1:4.5, 4.5:1, and 9: 1.

In some embodiments, the number of viable spores of the Bacillus amyloliquefaciens is 1 to 1000 hundred million/gram. In some embodiments, the number of viable spores of Bacillus amyloliquefaciens is 1000 hundred million/gram.

The invention also provides application of the bactericidal composition in preventing and treating fungal diseases of crops.

In some embodiments, the fungal diseases include gray mold, early blight, sclerotinia, and anthracnose, and the crops include fruit crops, vegetable crops, legume crops, oil crops, flower crops, and medicinal crops. The fruit crops comprise strawberries, grapes, oranges, apples and the like, the vegetable crops comprise cucumbers, tomatoes, hot peppers, potatoes and the like, the bean crops comprise beans, cowpeas, soybeans and the like, the oil crops comprise rapes, peanuts, sunflowers and the like, the flower crops comprise roses, roses and the like, and the medicinal crops comprise coptis chinensis, white atractylodes rhizome and the like.

The invention also provides a bactericide, which comprises the bactericidal composition and an auxiliary material acceptable in the agricultural pharmacy.

In some embodiments, the content of the bactericidal composition in the bactericide is 7% to 70% by mass.

In some embodiments, the bactericide is in the form of wettable powder, water dispersible granule, suspension or suspoemulsion.

In some embodiments, the agriculturally acceptable adjuvant is one or more of a wetting agent, a dispersing agent, a penetrating agent, an anti-freeze agent, a thickening agent, a synergist, a filler.

In some embodiments, the bactericide is wettable powder and consists of the following components in percentage by mass:

the wetting agent is one or more of alkyl naphthalene sulfonate, sodium dodecyl benzene sulfonate, sodium dodecyl naphthalene sulfonate and sodium lauryl sulfate, the dispersing agent is one or two of sodium lignin sulfonate and polycarboxylate, and the filler is one or two of sodium metasilicate and anhydrous sodium sulphate.

In some embodiments, when the bactericide is water dispersible granules, the bactericide comprises the following components in percentage by mass:

the wetting agent is one or more of alkyl naphthalene sulfonate, sodium dodecyl benzene sulfonate, sodium dodecyl naphthalene sulfonate and sodium lauryl sulfate, the dispersing agent is one or two of sodium lignosulfonate and polycarboxylate, the disintegrating agent is one or more of ammonium sulfate, sodium chloride and PVP, the adhesive is maltodextrin and/or soluble starch, and the filler is one or more of acerola powder, lactose and bentonite.

In some embodiments, the bactericide is in the form of a suspension agent and comprises the following components in percentage by mass:

the anti-freezing agent is preferably ethylene glycol and/or propylene glycol, the defoaming agent is an organic silicon defoaming agent, and the water is distilled water.

In some embodiments, the bactericide is in the form of a suspoemulsion, and consists of the following components in percentage by mass:

the anti-freezing agent is composed of an emulsifier, a solvent and a thickening agent, wherein the emulsifier is one or more of alkylphenol polyoxyethylene ether, triethanolamine salt and phenethylphenol polyoxyethylene ether, the dispersing agent is alkylaryl polyoxyethylene ether and/or sodium salt of a naphthalene sulfonic acid formaldehyde condensate, the solvent is dimethylformamide and/or dimethyl sulfoxide, the thickening agent is xanthan gum and/or sodium carboxymethylcellulose, the anti-freezing agent is ethylene glycol and/or propylene glycol, the defoaming agent is an organic silicon defoaming agent, water is distilled water, and oil is soybean oil, white oil and biodiesel.

In the present invention, the bacillus amyloliquefaciens is bacillus amyloliquefaciens powder, and can be obtained commercially or prepared by the method disclosed in the patent publication No. CN107988118A entitled "fermentation medium and fermentation method of a bacillus". Preferably, the number of viable spores of Bacillus amyloliquefaciens is 50 to 1000 hundred million/g.

In some embodiments, the bacillus amyloliquefaciens strain adopted in the wettable powder, the water dispersible granule, the suspending agent and the suspending emulsion provided by the invention is bacillus amyloliquefaciens 1841, and the number of viable spores is 1000 hundred million/g.

In the bactericide prepared by the invention, the number of viable spores of the bacillus amyloliquefaciens is 50-200 hundred million/g.

The invention provides a sterilization composition, application thereof and a bactericide. The bactericidal composition consists of bacillus amyloliquefaciens and iprodione. The results of indoor toxicity test tests show that when the mass ratio of the bacillus amyloliquefaciens to the iprodione is (1-9) to (1-9), the synergistic coefficients R are all larger than 1.5, and the synergistic effect is achieved. The field efficacy test shows that on the premise that the dosage of the active ingredients in the sterilization composition preparation provided by the invention is less than the dosage of the active ingredients of each single agent, the prevention effect is higher than that of each single agent, so that the dosage of the chemical bactericide is greatly reduced, the potential drug resistance risk of the existing pathogenic bacteria to iprodione is reduced, the effect of bacillus amyloliquefaciens on effectively controlling gray mold, early blight, sclerotinia and anthracnose is also exerted, the defect of slow control effect of bacillus amyloliquefaciens is compensated, and the advantages of the biocontrol sclerotium chemical bactericide are simultaneously integrated.

Detailed Description

The invention discloses a sterilization composition, application thereof and a sterilization agent, and a person skilled in the art can realize the sterilization composition by properly improving process parameters by referring to the content. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.

The description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

The invention is further illustrated by the following examples: