阅读说明：本技术 焦山楂碳量子点（纳米颗粒/纳米粒子）的制备及其应用 (Preparation and application of coke hawthorn carbon quantum dots (nano particles/nano particles) ) 是由 屈会化 赵琰 王庆国 孔慧 鲁放 孙紫薇 熊威 宋兴兴 曹鹏 成金俊 张美龄 于 2018-06-22 设计创作，主要内容包括：本发明涉及从焦山楂中提取分离纯化出的量子点(纳米颗粒/纳米粒子)的方法,该方法包括：焦山楂,经溶剂萃取或者提取,再用分子量大于1000Da的透析袋透析,或者超滤,得到焦山楂碳量子点,该碳量子点在紫外灯365nm下呈蓝色荧光、透射电镜测量粒径＜100nm,主要由C、H、O和N等元素构成；本发明还涉及此量子点在医药领域的应用,通过注射、口服或外用等途径给药后产生的降血糖、止血、镇痛、镇静、抗炎、抗过敏、抗氧化、解热、解痉、降糖、降压、对抗肿瘤化疗药物的副反应等。(The invention relates to a method for extracting, separating and purifying quantum dots (nano particles/nano particles) from scorched hawthorn, which comprises the following steps: extracting fructus crataegi with solvent, dialyzing with dialysis bag with molecular weight greater than 1000Da, or ultrafiltering to obtain fructus crataegi preparata carbon quantum dots which exhibit blue fluorescence under ultraviolet lamp 365nm and have particle diameter less than 100nm measured by transmission electron microscope and mainly comprise C, H, O and N; the invention also relates to the application of the quantum dot in the field of medicine, and the quantum dot is used for reducing blood sugar, stopping bleeding, easing pain, calming, resisting inflammation, resisting allergy, resisting oxidation, relieving fever, relieving spasm, reducing blood sugar, reducing blood pressure, resisting side reaction of tumor chemotherapy drugs and the like after being administrated by injection, oral administration or external application and other ways.)

1. A method for extracting and purifying quantum dots from fructus crataegi preparata is provided.

The preparation method of the parched fructus crataegi comprises parching, microwave heating, and decocting. Extracting with solvent, dialyzing with dialysis bag with molecular weight of 1-10 KDa, and collecting the fraction retained in the dialysis bag, or ultrafiltering with ultrafiltration tube of 10KD to obtain filtrate. The carbon quantum dots are characterized in that: blue fluorescence under an ultraviolet lamp of 365nm, and a transmission electron microscope for measuring the particle size of 1-100nm, wherein the particle size mainly comprises C, O, N element and a small amount of sulfur element.

2. Application of fructus crataegi preparata quantum dots in the field of medicine is provided.

Including but not limited to hemostasis, analgesia, sedation, anti-inflammation, anti-allergy, anti-oxidation, antipyretic, spasmolysis, hypoglycemic, antihypertensive, anti-side effects of tumor chemotherapy drugs.

3. The method for preparing scorched hawthorn fruit according to claim 1, wherein the temperature of the dry distillation method is 100-1500 ℃. The extraction solvent is one or more of water, methanol, ethanol, ethyl acetate, ethyl formate, methyl acetate, acetone, diethyl ether, chloroform, and petroleum ether.

4. The method for preparing scorched hawthorn fruit according to claim 3, wherein the temperature of the dry distillation method is 200-500 ℃.

5. The quantum dot of claim 1 having a particle size of 1-20 nm. The elemental composition of quantum dots includes, but is not limited to C, H, O, N and the S element.

6. The use of claim 2, wherein the dosage form is injection, powder for injection, oral liquid, hard capsule, soft capsule, tablet, suppository, water-paste pill, honeyed pill, concentrated pill, ointment, powder, emulsion, paste, and liniment.

7. The use according to claim 2 for the treatment or prevention of a condition which is: dyspepsia, anorexia, etc.; diabetes, hyperglycemia; various bleeding diseases, blood vessel burst, cerebral hemorrhage, liver hemorrhage, pulmonary hemorrhage, renal hemorrhage, splenic hemorrhage, biliary hemorrhage, retinal hemorrhage, nasal hemorrhage, oral hemorrhage, digestive hemorrhage, gastric hemorrhage, intestinal hemorrhage, rectal hemorrhage, puerperal hemorrhage, metrorrhagia, postoperative hemorrhage, traumatic hemorrhage, etc.; pain due to internal or external injury, cancer pain, headache, eye pain, ear pain, nasal pain, oral pain, skin pain, heart pain, liver pain, stomach pain, lung pain, visceral pain, limb pain; allergic rhinitis, asthma, eczema, urticaria, purpura, drug allergy, food allergy, ultraviolet allergy, allergic constitution, various allergies and anaphylactoid; mania, insomnia, anxiety, depression; fever, high fever of unknown cause, low fever; burn, scald, convulsion, epilepsy, hypertension, headache, nausea, emesis, edema, and apoplexy due to tumor chemotherapy.

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to preparation of coke hawthorn carbon quantum dots and application of the coke hawthorn carbon quantum dots in the field of medicines.

Background

Haw, originally recorded in Xin Xiu Ben Cao of Tang, is a traditional food-digesting medicine with a history of more than a thousand years of use, and its processing method is recorded in the classic medical record of the past generation and has abundant resources.

The method for moxibustion of hawthorn mainly comprises stir-frying (stir-frying to yellow), stir-frying to brown, carbonizing, stir-frying with auxiliary materials, steaming and the like. The stir-frying method is originally found in the Ming Dynasty 'Danxi Xin Fa' (Hawthorn fruit: stir-frying, its posterity (compendium of medicine), Qing Dynasty (Collection of entagles) and Wen Bing (treatise on epidemic febrile diseases) are recorded with stir-fried haw or haw pulp. In Yunnan materia Medica, there are "Hawthorn seeds: the record of stir-frying yellow is recorded in the book of treatise on doctor Zong and the book of dialectical warm diseases in the Qing Dynasty, while the record of stir-frying black in the book of Ming Dynasty, the record of stir-frying black in powder and stir-frying black in powder can treat blood stasis (the book of treatise on doctor Zong) mainly aims at changing the property of medicine and facilitating the dispensing. The records of charred Haw fruit appear in the documents of the Qing Dynasty, Wuwei Tong medical records and so on. The steaming method is originally found in the Ministry of the Heart of Danxi (Danxi Heart method) and hawthorn: steaming and drying in the sun. From the Ming Dynasty, there are four kinds of auxiliary materials and five kinds of preparation methods in the past literature of hawthorn processing with auxiliary materials. Ming Dynasty, the regulation and treatment standard rope, is recorded with vinegar-fried. The Qing Dynasty, the Yi Zong jin Jian, is fried with wine, the Wen Shi Yi, is fried with ginger juice, and the Qing Dynasty, the Ben Jing Jie Yuan, is soaked with urine and enucleation. Modern processed products of hawthorn mainly comprise: cleaning fructus crataegi, parched fructus crataegi, and fructus crataegi preparata. At present, the research make internal disorder or usurp on stir-frying is mainly focused on the change of chemical components, but no clear answer is provided for the material basis and principle of the pharmacodynamic action of stir-frying.

Disclosure of Invention

The invention extracts and separates carbon quantum dots (nano particles/nano particles) from the scorched hawthorn for the first time, and discovers that the scorched hawthorn carbon quantum dots have strong biological effects of inhibiting intestinal canal disaccharidase and inhibiting sucrase and maltase in vitro for the first time.

The technical scheme is as follows: the invention provides a method for extracting and separating carbon quantum dots from scorched hawthorn fruits. The invention also provides the biological activity of the charred hawthorn carbon quantum dots and the application thereof in the field of medicine.

The preparation method specifically comprises the following steps: the charred hawthorn is prepared by a baking method, carbon quantum dots are extracted from the charred hawthorn by using a solvent, and the high-purity carbon quantum dots are obtained by further separation and purification including dialysis, ultrafiltration, chromatography and other technical methods.

Drawings

FIG. 1 is a diagram of UV, fluorescence, X-ray diffraction and infrared spectrums of coke hawthorn carbon quantum dots

FIG. 2 is an X-ray photoelectron spectrum of coke hawthorn carbon quantum dots

Detailed Description