阅读说明：本技术 一种胶原蛋白的提取系统 (Collagen extraction system ) 是由 祁松 于 2018-06-26 设计创作，主要内容包括：本发明公开了一种胶原蛋白提取系统,包括依次相互连通的以下装置：对原料进行脱脂的脱脂槽和淋洗装置,对脱脂后原料进行粉碎的破碎机,对破碎后的原料进行酶解处理的酶解罐,对酶解液进行冷冻离心处理的冷冻离心机,对冷冻离心得到的沉淀物进行碱洗处理的碱洗罐,对碱洗液进行盐析处理的盐析单元以及收集盐析后的粗胶原的收集器。本发明的胶原蛋白提取系统,结构简单,提取效率高,适合各种来源的胶原蛋白的提取。(The invention discloses a collagen extraction system, which comprises the following devices which are communicated with each other in sequence: the device comprises a degreasing tank and a leaching device for degreasing raw materials, a crusher for crushing the degreased raw materials, an enzymolysis tank for performing enzymolysis treatment on the crushed raw materials, a refrigerated centrifuge for performing refrigerated centrifugation treatment on enzymolysis liquid, an alkaline washing tank for performing alkaline washing treatment on precipitates obtained by refrigerated centrifugation, a salting-out unit for performing salting-out treatment on alkaline washing liquid and a collector for collecting crude collagen after salting-out. The collagen extraction system disclosed by the invention is simple in structure, high in extraction efficiency and suitable for extracting collagen from various sources.)

1. The collagen extraction system is characterized by comprising the following devices which are sequentially communicated with each other: the device comprises a degreasing tank and a leaching device for degreasing raw materials, a crusher for crushing the degreased raw materials, an enzymolysis tank for performing enzymolysis treatment on the crushed raw materials, a refrigerated centrifuge for performing refrigerated centrifugation treatment on enzymolysis liquid, an alkaline washing tank for performing alkaline washing treatment on precipitates obtained by refrigerated centrifugation, a salting-out unit for performing salting-out treatment on alkaline washing liquid and a collector for collecting crude collagen after salting-out.

2. The collagen extraction system according to claim 1, wherein a stirring device is disposed in said enzymatic tank.

3. The collagen extraction system according to claim 1, wherein a constant temperature device is disposed in said enzymatic hydrolysis tank.

4. The collagen extraction system according to claim 1, wherein a water washing unit and a freeze drying unit are further provided between the salting-out unit and the collector.

Technical Field

The invention relates to the field of collagen extraction and processing, in particular to a collagen extraction system.

Background

As is well known, the existing collagen process is: in the extraction process of the collagen, firstly, adding a proper amount of water into the raw materials, mixing, steaming at high temperature, adding a proper amount of enzymolysis agent under a proper pH condition, carrying out enzymolysis extraction, and carrying out fine filtration after completion; and (3) concentrating and dehydrating the filtrate in an evaporation concentration system, and after the concentrated liquid reaches a certain concentration, feeding the concentrated liquid into spray drying equipment to obtain powder, namely the collagen.

However, the conventional collagen preparation process has many defects, such as: (1) the volume of the extracting solution is large, and the production period of the product is long; (2) the enzymolysis liquid does not thoroughly remove impurities, so that the quality of the collagen is influenced; (3) the resulting finished product often has a bitter and fishy smell; (4) the salt content is high.

Disclosure of Invention

The technical problem to be solved by the invention is to provide an extraction system of collagen, which has the advantages of simple structure, high extraction speed and high efficiency, and is suitable for extracting collagen from various sources.

In order to solve the technical problem, the invention provides a collagen extraction system, which comprises the following devices which are sequentially communicated with each other: the device comprises a degreasing tank and a leaching device for degreasing raw materials, a crusher for crushing the degreased raw materials, an enzymolysis tank for performing enzymolysis treatment on the crushed raw materials, a refrigerated centrifuge for performing refrigerated centrifugation treatment on enzymolysis liquid, an alkaline washing tank for performing alkaline washing treatment on precipitates obtained by refrigerated centrifugation, a salting-out unit for performing salting-out treatment on alkaline washing liquid and a collector for collecting crude collagen after salting-out.

In a preferred embodiment of the present invention, a stirring device is disposed in the enzymolysis tank.

In a preferred embodiment of the present invention, a constant temperature device is disposed in the enzymolysis tank.

In a preferred embodiment of the present invention, a water washing unit and a freeze-drying unit are further disposed between the salting-out unit and the collector.

The invention has the beneficial effects that:

according to the collagen extraction and purification system, the collagen is extracted by an enzymolysis method, so that the problems of collagen denaturation caused by acid-base hydrolysis and damage caused by an amino group molecular structure are solved, and a natural active triple-helix structure is preserved. Secondly, the extraction system has simple structure, high extraction speed and high efficiency, and is suitable for extracting collagen from various sources.

Drawings

FIG. 1 is a schematic diagram of an extraction system according to an embodiment of the invention.

Detailed Description

The present invention is further described below in conjunction with the following figures and specific examples so that those skilled in the art may better understand the present invention and practice it, but the examples are not intended to limit the present invention.

All publications, patent applications, patents, and other references mentioned in this specification are herein incorporated by reference in their entirety. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In case of conflict, the present specification, including definitions, will control.

When the specification concludes with claims with the heading "known to those skilled in the art", "prior art", or the like, to derive materials, substances, methods, procedures, devices, or components, etc., it is intended that the subject matter derived from the heading encompass those conventionally used in the art at the time of filing this application, but also include those that are not currently in use, but would become known in the art to be suitable for a similar purpose.

In the context of the present specification, anything or things which are not mentioned, except where explicitly stated, are directly applicable to those known in the art without any changes. Moreover, any embodiment described herein may be freely combined with one or more other embodiments described herein, and the technical solutions or concepts resulting therefrom are considered part of the original disclosure or original disclosure of the invention, and should not be considered as new matters not disclosed or contemplated herein, unless a person skilled in the art would consider such a combination to be clearly unreasonable.

Referring to fig. 1, the present embodiment provides a collagen extraction system, which includes the following devices sequentially connected to each other: the device comprises a degreasing tank and a leaching device for degreasing raw materials, a crusher for crushing the degreased raw materials, an enzymolysis tank for performing enzymolysis treatment on the crushed raw materials, a refrigerated centrifuge for performing refrigerated centrifugation treatment on enzymolysis liquid, an alkaline washing tank for performing alkaline washing treatment on precipitates obtained by refrigerated centrifugation, a salting-out unit for performing salting-out treatment on alkaline washing liquid and a collector for collecting crude collagen after salting-out.

Wherein the raw materials are rich collagen-containing, low-cost and easily available materials such as fish skin, pigskin and the like, and are added into a degreasing tank, chloroform/ethanol mixed liquor is added for degreasing treatment, and then distilled water is sprayed by a spraying device to remove the chloroform/ethanol mixed liquor and grease remained on the surface of the raw materials. The degreased raw materials are conveyed to a high-speed crusher to be mashed into paste, and then enter an enzymolysis tank, and a stirring device is arranged in the enzymolysis tank to stir enzymolysis liquid, so that enzymolysis reaction is fully performed, and the extraction rate of collagen is improved. The enzymolysis tank is also provided with a constant temperature device, the constant temperature device enables the temperature of the enzymolysis liquid to be between 35 and 45 ℃ all the time, the enzyme is in a high-activity temperature range, and the high-speed proceeding of the enzymolysis reaction is ensured.

The enzymatic hydrolysate enters a refrigerated centrifuge for refrigerated centrifugation treatment, and the obtained precipitate enters an alkaline washing tank to be dissolved; the dissolution solution is then fed to a salting-out unit for salting-out treatment, whereby crude collagen is obtained, which is then collected in a collector.

Crude collagen has many impurities and cannot be directly applied. Therefore, in this embodiment, a water washing unit and a freeze-drying unit are further disposed between the salting-out unit and the collector, wherein the water washing unit dissolves the obtained crude collagen in hot water to obtain a collagen solution; and (3) the collagen solution is subjected to primary filtration and then enters a freeze drying unit to obtain the primarily purified collagen.

The collagen extraction and purification system of this embodiment adopts enzymolysis method to extract, avoids acid-base hydrolysis to lead to collagen denaturation, and amino group molecular structure causes the problem of destruction, has preserved natural active triple helix structure. Secondly, the extraction system has simple structure, high extraction speed and high efficiency, and is suitable for extracting collagen from various sources.

The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. The equivalent substitution or change made by the technical personnel in the technical field on the basis of the invention is all within the protection scope of the invention. The protection scope of the invention is subject to the claims.