阅读说明：本技术 糖化和发酵纤维素材料的方法 (Methods of saccharifying and fermenting cellulosic material ) 是由 J·弗里克曼 A·R·加斯帕尔 M·史蒂文斯 徐辉 K·S·约翰森 于 2014-02-21 设计创作，主要内容包括：本发明涉及糖化一种纤维素材料的方法,这些方法包括在浓度处于0.5％至10％的饱和度范围内的溶解氧的存在下使该纤维素材料经受一种纤维素分解酶组合物和一种GH61多肽以及任选地一种过氧化氢酶。本发明还涉及使用包括本发明的糖化步骤的方法生产所希望的发酵产物如乙醇的方法。(The present invention relates to methods of saccharifying a cellulosic material comprising subjecting the cellulosic material to a cellulolytic enzyme composition and a GH61 polypeptide and optionally a catalase in the presence of dissolved oxygen at a concentration in the saturation range of 0.5% to 10%. The invention also relates to a process for producing a desired fermentation product, such as ethanol, using a process comprising the saccharification step of the invention.)

1.A method of saccharifying a cellulosic material, comprising subjecting the cellulosic material to a cellulolytic enzyme composition and a G61 polypeptide in a vessel, wherein oxygen is added to the vessel to maintain a dissolved oxygen concentration in the range of 0.5% to 10% saturation.

2. The method of claim 1, wherein the cellulosic material is further subjected to a catalase.

3. The method of claim 1, wherein the amount of catalase is in the range of 0.5% to 25% of the total protein.

4. The method of claim 1, further comprising adding a base during saccharification to maintain the pH in the range of about 3.0 to 7.0.

5. The method of claim 4, wherein the base is selected from the group consisting of: KOH, NaOH, Ca (OH)₂And NH₄OH。

6. The method of claim 1, wherein the cellulolytic enzyme composition comprises a cellobiohydrolase, an endoglucanase, and a beta-glucosidase.

7. The method of claim 1, wherein the cellulolytic enzyme composition comprises a cellobiohydrolase I, a cellobiohydrolase II, an endoglucanase, and a beta-glucosidase.

8. The method of claim 1, wherein the cellulolytic enzyme composition comprises a cellobiohydrolase I, a cellobiohydrolase II, an endoglucanase, a beta-glucosidase, and a xylanase.

9. The method of claim 1, wherein the cellulolytic enzyme composition comprises a cellobiohydrolase I, a cellobiohydrolase II, an endoglucanase, a beta-glucosidase, a xylanase, and a beta-xylosidase.

10. The method of claim 1, wherein the container comprises more than 10m³。

11. The method of claim 2, further comprising adding a base during saccharification to maintain the pH in the range of about 3.0 to 7.0.

Summary of The Invention

Described herein are methods of saccharifying a cellulosic material to fermentable sugars. Also described are methods of producing a fermentation product (e.g., ethanol) from a cellulosic material (e.g., a pretreated cellulosic material) by saccharification and fermentation.

In one aspect, the invention relates to methods of saccharifying a cellulosic material comprising subjecting the cellulosic material to a cellulolytic enzyme composition and a GH61 polypeptide in the presence of dissolved oxygen at a concentration in the saturation range of 0.5% to 10%.

In another aspect, the invention relates to methods of producing a fermentation product, the methods comprising:

(a) subjecting a cellulosic material to a cellulolytic enzyme composition and a GH61 polypeptide in the presence of dissolved oxygen at a concentration in the saturation range of 0.5% -10%;

(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms; and is

(c) Recovering the fermentation product from (b).

In another aspect, the invention relates to methods of producing a fermentation product, the methods comprising:

(a) subjecting a cellulosic material to a cellulolytic enzyme composition, a GH61 polypeptide, and a catalase in the presence of dissolved oxygen at a concentration in the saturation range of 0.5% -10%;

(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms; and is

(c) Recovering the fermentation product from (b).

In one embodiment, the cellulosic material has been pretreated, for example, by chemical and/or physical pretreatment (such as dilute acid and/or steam explosion pretreatment). In a preferred embodiment, the cellulosic material is unwashed, such as unwashed pretreated corn stover (uwPCS).

The pretreated cellulosic material is saccharified/hydrolyzed to sugars using the methods of the present invention. These sugars can be converted into a number of useful desirable substances, such as fuels, potable ethanol, and/or fermentation products (e.g., acids, alcohols, ketones, gases, etc.).

The saccharified pretreated cellulosic material can be sugars that can be used in processes for producing syrups (e.g., High Fructose Corn Syrup (HFCS)) and/or plastics (e.g., polyethylene, polystyrene, and polypropylene), polylactic acid (e.g., for producing PET).