Magnetic particle light-emitting double-layer micro-fluidic chip and detection system
阅读说明：本技术 一种磁微粒发光双层微流控芯片以及检测系统 (Magnetic particle light-emitting double-layer micro-fluidic chip and detection system ) 是由 王东 范玉霞 李泉 于 2019-10-11 设计创作，主要内容包括：本发明属于微流控芯片发光免疫检测技术领域,尤其涉及一种磁微粒发光双层微流控芯片以及检测系统。芯片包括顶板和底板,顶板包括加样部、标记配体存储部和样本混合区,样本混合区分别与加样部与标记配体存储部相互连通；底板包括导流区、磁颗粒包被部、清洗区、检测区和清洗液存储部,导流区内部设有高度低于磁颗粒包被部底璧的凹槽以及设在凹槽上并连接磁颗粒包被部的导流部。样本从加样部进入,在样本混合区与标记配体相互混合,再进入导流区的凹槽,需通过毛细作用才能够吸走凹槽中的样本,并且由于截断槽和阻断部的作用,样本只能从导流部进入磁颗粒包被部,再与磁颗粒配体混合反应,并在清洗区清洗之后,在检测区实现发光检测。(The invention belongs to the technical field of micro-fluidic chip luminescence immunodetection, and particularly relates to a magnetic particle luminescence double-layer micro-fluidic chip and a detection system. The chip comprises a top plate and a bottom plate, wherein the top plate comprises a sample adding part, a labeled ligand storage part and a sample mixing region, and the sample mixing region is communicated with the sample adding part and the labeled ligand storage part respectively; the bottom plate comprises a flow guide area, a magnetic particle coating part, a cleaning area, a detection area and a cleaning liquid storage part, wherein a groove with the height lower than the bottom wall of the magnetic particle coating part and a flow guide part which is arranged on the groove and connected with the magnetic particle coating part are arranged in the flow guide area. The sample enters from the sample adding part, is mixed with the labeled ligand in the sample mixing area and then enters the groove of the flow guide area, the sample in the groove can be absorbed by capillary action, and due to the action of the cut-off groove and the blocking part, the sample can only enter the magnetic particle coating part from the flow guide part, then is mixed and reacts with the magnetic particle ligand, and after the cleaning area is cleaned, the luminescence detection is realized in the detection area.)
1. A magnetic particle light-emitting double-layer micro-fluidic chip is characterized by comprising:
the top plate comprises an adding part, a labeled ligand storage part and a sample mixing region, wherein a labeled ligand is arranged in the labeled ligand storage part, and the sample mixing region is communicated with the adding part and the labeled ligand storage part respectively;
the bottom plate is arranged on the top plate and comprises a flow guide area communicated with the sample mixing area, a magnetic particle coating part communicated with the flow guide area, a cleaning area communicated with the magnetic particle coating part, a detection area communicated with the cleaning area and a cleaning solution storage part communicated with the cleaning area, a groove lower than the bottom wall of the magnetic particle coating part, a flow guide part arranged on the groove and connected with the magnetic particle coating part, a cutting groove arranged below the front end of the flow guide part and a blocking part arranged on the flow guide part are arranged in the flow guide area, a magnetic particle ligand solution is arranged in the magnetic particle coating part, and a cleaning solution is arranged in the cleaning solution storage part.
2. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the top plate further comprises an air pump communicated with the sample adding part.
3. The magnetic particle light-emitting double-layer microfluidic chip according to claim 2, wherein the top plate is provided with an elastic member at a position corresponding to the air pump and the sample mixing region.
4. The magnetic particle light-emitting double-layer microfluidic chip according to claim 2, wherein a porous elastic member is disposed inside the air pump.
5. The magnetic particle light-emitting double-layer microfluidic chip according to claim 1, wherein the sample application part comprises a sample application port and a cover for opening or closing the sample application port, and the sample application part further comprises a rubber ring arranged on the sample application port.
6. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the top plate and the bottom plate are provided with limiting notches at positions corresponding to each other.
7. The magnetic particle light-emitting double-layer microfluidic chip according to claim 1, wherein the top plate is provided with a first buckle or a first buckle, the bottom plate is provided with a second buckle or a second buckle, and the top plate and the bottom plate are buckled with each other through the mutual matching of the first buckle and the second buckle or the mutual matching of the first buckle and the second buckle.
8. The magnetic particle light-emitting double-layer microfluidic chip according to claim 1, wherein a single-sided adhesive substance is provided on a part or all of at least one side of the bottom plate.
9. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein a product label is disposed on the surface of the top plate or the bottom plate, and a two-dimensional code label is disposed on the surface of the top plate or the bottom plate.
10. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the top plate is provided with magnetic attraction yielding holes on corresponding communication tracks with the magnetic particle coating part, the cleaning area and the detection area.
11. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the base plate further comprises a waste reservoir in communication with the cleaning region.
12. The magnetic particle light-emitting double-layer microfluidic chip according to any one of claims 1 to 11, wherein the bottom plate further comprises a light-emitting liquid storage portion communicated with the detection region, and a light-emitting liquid is disposed inside the light-emitting liquid storage portion.
13. The magnetic particle light-emitting double-layer microfluidic chip according to claim 12, wherein the top plate is provided with a cleaning solution relief hole and a light-emitting solution relief hole at corresponding positions of the cleaning solution storage part and the light-emitting solution storage part.
14. The magnetic particle light-emitting double-layer microfluidic chip according to any one of claims 1 to 11, wherein a fluorescent liquid is provided inside the labeled ligand storage part.
15. A magnetic particle light emitting dual layer microfluidic detection system, the detection system comprising:
the magnetic particle light-emitting double-layer microfluidic chip of any one of claims 1 to 14;
the magnet unit is used for driving the magnetic particles in the magnetic particle ligand solution to move;
a squeezing unit for squeezing the labeled ligand storage part and the cleaning solution storage part to make the labeled ligand and the cleaning solution flow out;
a detection unit for detecting a luminescence signal in the detection zone.
Technical Field
The invention belongs to the technical field of micro-fluidic chip luminescence immunodetection, and particularly relates to a magnetic particle luminescence double-layer micro-fluidic chip and a detection system.
Background
Currently, there are two major trends In Vitro Diagnostics (IVD): one is automatic and integrated, namely, the high-precision disease analysis and diagnosis is realized by utilizing full-automatic and high-sensitivity large-scale instruments and equipment of a central laboratory matched with a large-scale hospital; the other type of miniaturization and bedside miniaturization is realized, namely, the rapid analysis and diagnosis on site is realized through handheld small simple equipment. However, small hospitals are not capital intensive, have a small sample size, and are not suitable for purchasing expensive large equipment. Therefore, most of the rapid detection equipment adopted by hospitals at the present stage mainly comprises test strips and corollary equipment thereof, but the test strips can only realize qualitative or semi-quantitative detection, and have the advantages of low detection sensitivity, poor specificity, poor repeatability and obvious interference. Due to the fact that China has a large population, aging is aggravated, the disease incidence is increased sharply, and dependence on large hospitals is overwhelmed. Therefore, the development of a rapid detection method and equipment which are simple and convenient to operate, high in sensitivity, good in repeatability and accurate in quantification becomes urgent.
The micro-fluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the biological, chemical and medical analysis process into a micron-scale chip, and automatically completes the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine, etc., it has been developed into a research field with multiple interdisciplinary disciplines of biology, chemistry, medicine, fluid, material, machinery, etc., and is applied to the fields of biomedical research, biochemical detection, judicial appraisal, etc. However, the existing microfluidic chip is provided with a top plate and a bottom plate, when a sample flows from the top plate to the bottom plate, the sample can automatically flow backwards along the channel due to the gravity action because the channels of the bottom plate are at the same level. In some detection items, detection and analysis can be completed only by taking a small amount of sample, and if the sample flows from the top plate to the bottom plate and directly flows in the bottom plate, the final detection result is greatly influenced, and the detection result is wrong. In addition, the sample flows through the channel of the bottom plate, the flow path of the sample cannot be limited, the sample flowing to the detection area cannot be completely filtered, and the final detection result is further influenced.
Disclosure of Invention
The embodiment of the invention provides a magnetic particle light-emitting double-layer micro-fluidic chip and a detection system, and aims to solve the problems that a sample in the existing micro-fluidic chip directly flows in a bottom plate after flowing from a top plate to the bottom plate, and the flowing path of the sample cannot be limited.
The embodiment of the invention is realized by providing a magnetic particle light-emitting double-layer micro-fluidic chip, which comprises: the top plate comprises an adding part, a labeled ligand storage part and a sample mixing region, wherein a labeled ligand is arranged in the labeled ligand storage part, and the sample mixing region is communicated with the adding part and the labeled ligand storage part respectively; the bottom plate is arranged on the top plate and comprises a flow guide area communicated with the sample mixing area, a magnetic particle coating part communicated with the flow guide area, a cleaning area communicated with the magnetic particle coating part, a detection area communicated with the cleaning area and a cleaning solution storage part communicated with the cleaning area, a groove lower than the bottom wall of the magnetic particle coating part, a flow guide part arranged on the groove and connected with the magnetic particle coating part, a cutting groove arranged below the front end of the flow guide part and a blocking part arranged on the flow guide part are arranged in the flow guide area, a magnetic particle ligand solution is arranged in the magnetic particle coating part, and a cleaning solution is arranged in the cleaning solution storage part.
Furthermore, the top plate also comprises an air pump which is mutually communicated with the sample adding part.
Furthermore, the top plate is provided with an elastic member at a position corresponding to the air pump and the sample mixing area.
Further, a porous elastic member is provided inside the air pump.
Furthermore, the sample adding part comprises a sample adding port and a sealing cover for opening or sealing the sample adding port, and the sample adding part also comprises a rubber ring arranged on the sample adding port.
Furthermore, the top plate and the bottom plate are provided with limiting notches at corresponding positions.
Furthermore, a first buckle or a first clamping groove is arranged on the top plate, a second clamping groove or a second buckle is arranged on the bottom plate, and the top plate and the bottom plate are buckled with each other through the mutual matching of the first buckle and the second clamping groove or the mutual matching of the first clamping groove and the second buckle.
Furthermore, a part or all of the area of at least one side of the bottom plate is provided with a single-sided adhesive substance.
Furthermore, a product label is arranged on the surface of the top plate or the bottom plate, and a two-dimensional code label is arranged on the surface of the top plate or the bottom plate.
Furthermore, the top plate is provided with magnetic attraction yielding holes on corresponding communication tracks of the magnetic particle coating part, the cleaning area and the detection area.
Further, the bottom plate further comprises a waste liquid pool communicated with the cleaning area.
Furthermore, the bottom plate further comprises a luminous liquid storage part which is communicated with the detection area, and luminous liquid is arranged in the luminous liquid storage part.
Furthermore, the top plate is provided with a cleaning liquid relief hole and a luminous liquid relief hole at corresponding positions of the cleaning liquid storage part and the luminous liquid storage part.
Further, a fluorescent liquid is provided inside the labeled ligand storage part.
The invention also provides a magnetic particle light-emitting double-layer micro-fluidic detection system, which comprises: the magnetic particle light-emitting double-layer micro-fluidic chip is described above; the magnet unit is used for driving the magnetic particles in the magnetic particle ligand solution to move; a squeezing unit for squeezing the labeled ligand storage part and the cleaning solution storage part to make the labeled ligand and the cleaning solution flow out; a detection unit for detecting a luminescence signal in the detection zone.
The magnetic particle light-emitting double-layer micro-fluidic chip and the detection system have the advantages that a sample enters from the sample adding part, is mixed with a labeled ligand in the sample mixing area and then enters the groove of the flow guide area, the sample in the groove can be sucked away through capillary action due to the fact that the groove is lower than the bottom wall of the magnetic particle coating part, the sample can only enter the magnetic particle coating part from the flow guide part due to the effects of the cut-off groove and the cut-off part and then is fully mixed and reacted with the magnetic particle ligand, and light-emitting detection is achieved in the detection area after the cleaning area is cleaned by cleaning liquid.
Drawings
Fig. 1 is an exploded schematic view of a magnetic particle light-emitting double-layer microfluidic chip provided in an embodiment of the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1;
fig. 3 is another exploded view of the magnetic particle light-emitting double-layer microfluidic chip according to the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
According to the invention, by designing the magnetic particle light-emitting double-layer micro-fluidic chip and the detection system, a sample enters from the