阅读说明：本技术 使用具有用于增加荧光亮度的接头和酶促可释放荧光部分的缀合物的可逆细胞检测 (Reversible cell detection using conjugates with linkers for increasing fluorescence brightness and enzymatically releasable fluorescent moieties ) 是由 C·杜斯 J·潘克拉茨 于 2019-04-23 设计创作，主要内容包括：本发明针对用于在细胞上标记目标部分的缀合物,其特征为具有通式(I)(X-(o)-L)-(n)–P–Y-(m),其中Y：识别目标部分的抗原识别部分,P：酶促可降解间隔基,X：荧光部分,L：包含一个或多个聚乙二醇残基的接头单元,n、m：1到100之间的整数,o：1到100之间的整数,其中L共价结合荧光部分X和酶促可降解间隔基P,且Y共价地结合至酶促可降解间隔基P,且其中酶促可降解间隔基P选自多糖、聚酯、核酸及其衍生物。在生物样本样品中用缀合物检测目标部分的方法。(The present invention is directed to conjugates for labeling a target moiety on a cell characterized by having the general formula (I) (X) o ‑L) n –P–Y m Wherein Y: an antigen recognition moiety that recognizes a target moiety, P: enzymatically degradable spacer, X: fluorescent moiety, L: a linker unit comprising one or more polyethylene glycol residues, n, m: integer between 1 and 100, o: 1 to 100, wherein L is covalently bound to the fluorescent moiety X and the enzymatically degradable spacer P, and Y is covalently bound to the enzymatically degradable spacer P, and wherein the enzymatically degradable spacer P is selected from the group consisting of polysaccharides, polyesters, nucleic acids and derivatives thereof. A method for detecting a target moiety with a conjugate in a biological sample.)