阅读说明：本技术 一种大球盖菇工厂化生产液体培养基及其制备方法 (liquid culture medium for industrialized production of stropharia rugoso-annulata and preparation method thereof ) 是由 刘记水 于 2019-08-22 设计创作，主要内容包括：本发明公开了一种大球盖菇工厂化生产液体培养基,由下述配比的原料组成：大豆粉4-6g/L、玉米粉4-6g/L、麸皮粉4-6g/L、白砂糖25-35g/L、磷酸二氢钾0.5-1g/L、硫酸镁0.5-1g/L、蛋白胨0.3-1g/L；本发明还公开了一种大球盖菇工厂化生产液体培养基的制备方法,包括以下步骤：原料研磨过筛、搅拌溶解、调整酸碱度、磁力搅拌和高压灭菌；本发明提供的一种大球盖菇工厂化生产液体培养基,培养基原材料来源丰富,培养基营养丰富、生长周期短,满足大球盖菇生长的营养需求；本配方应用在大球盖菇工厂化液体菌种生产中,具有营养丰富、液体菌种菌含量高、菌球成型、大小均匀,接入栽培料后发菌快速,有利于提高大球盖菇的单产和品质的特点。(The invention discloses a liquid culture medium for industrialized production of stropharia rugoso-annulata, which consists of the following raw materials in percentage by weight: 4-6g/L of soybean meal, 4-6g/L of corn meal, 4-6g/L of bran powder, 25-35g/L of white granulated sugar, 0.5-1g/L of monopotassium phosphate, 0.5-1g/L of magnesium sulfate and 0.3-1g/L of peptone; the invention also discloses a preparation method of the liquid culture medium for the industrialized production of stropharia rugoso-annulata, which comprises the following steps: grinding and sieving raw materials, stirring for dissolving, adjusting pH value, magnetically stirring and sterilizing under high pressure; the liquid culture medium for the industrial production of the stropharia rugoso-annulata, provided by the invention, has the advantages of rich raw material sources of the culture medium, rich nutrition of the culture medium and short growth period, and meets the nutritional requirements of the growth of the stropharia rugoso-annulata; the formula is applied to the industrialized liquid spawn production of the stropharia rugoso-annulata, and has the characteristics of rich nutrition, high spawn content of the liquid spawn, pellet forming, uniform size, quick spawn running after the cultivation material is inoculated, and contribution to improving the unit yield and the quality of the stropharia rugoso-annulata.)

1. a liquid culture medium for the industrialized production of stropharia rugoso-annulata is characterized by comprising the following raw materials in proportion: 4-6g/L of soybean meal, 4-6g/L of corn meal, 4-6g/L of bran powder, 25-35g/L of white granulated sugar, 0.5-1g/L of monopotassium phosphate, 0.5-1g/L of magnesium sulfate and 0.3-1g/L of peptone.

2. the liquid culture medium for the industrialized production of stropharia rugoso-annulata according to claim 1, which is characterized by comprising the following raw materials in proportion: 6g/L of soybean meal, 6g/L of corn meal, 6g/L of bran powder, 30g/L of white granulated sugar, 1g/L of monopotassium phosphate, 0.6g/L of magnesium sulfate and 0.3g/L of peptone.

3. The liquid culture medium for the industrialized production of stropharia rugoso-annulata according to claim 1, which is characterized by comprising the following raw materials in proportion: 5g/L of soybean meal, 5g/L of corn meal, 5g/L of bran powder, 35g/L of white granulated sugar, 1g/L of monopotassium phosphate, 0.6g/L of magnesium sulfate and 0.6g/L of peptone.

4. The liquid culture medium for the industrialized production of stropharia rugoso-annulata according to claim 1, which is characterized by comprising the following raw materials in proportion: 6g/L of soybean meal, 6g/L of corn meal, 6g/L of bran powder, 30g/L of white granulated sugar, 0.6g/L of monopotassium phosphate, 0.6g/L of magnesium sulfate and 0.6g/L of peptone.

5. A method for preparing a liquid medium for the industrial production of Stropharia rugosoannulata according to any one of claims 1 to 4, comprising the steps of:

S1: respectively grinding soybean powder, corn flour, bran powder, white granulated sugar, potassium dihydrogen phosphate, magnesium sulfate and peptone, and then crushing and sieving by using a 200-mesh sieve with a pore diameter;

S2: accurately weighing the raw materials according to the formula proportion, and putting the raw materials into a barrel to be stirred and dissolved uniformly;

s3: fixing the volume to the volume of the prepared culture medium, and adjusting the pH value to 6.0-6.2;

s4: subpackaging the liquid culture medium with the constant volume into a triangular flask with the volume of 1000ml by using a subpackaging device, putting a magnetic stirrer into each flask with the liquid content of 600m l, and plugging a tampon;

s5: the liquid medium prepared in S4 was sterilized in an autoclave.

Technical Field

The invention belongs to the technical field of edible mushroom cultivation, and particularly relates to a liquid culture medium for industrial production of stropharia rugoso-annulata and a preparation method thereof.

Background

Stropharia rugosoannulata is also called crinkle stropharia rugoso-annulata, russula vinosa, brown stropharia rugoso-annulata, Fritillaria deltoid, kidney-tonifying mushroom, Caiyun mushroom, etc., which is commonly called as "red pine mushroom". The mushroom has bright color, beautiful mushroom shape, soft and smooth cover, crisp stem, fragrant taste and rich nutrition, and is deeply welcomed by consumers. The stropharia rugoso-annulata is one of characteristic mushrooms recommended and cultivated by international food and agriculture organization to developing countries and one of ten traded mushrooms in the international edible mushroom market, stropharia rugoso-annulata is a hand for biologically decomposing crop leftovers, and the stropharia rugoso-annulata takes straws and manure of grains as main cultivation materials, so that the problem of environmental pollution is solved while the mushrooms are cultivated, and the stropharia rugoso-annulata becomes a vitality army of ecological agriculture.

In recent years, due to rapid development of the edible mushroom industry, particularly intensification of protection areas such as sheds and the like, and rapid expansion of the industrial edible mushroom industry, the stropharia rugosoannulata is relatively delayed in development, and the research on the stropharia rugosoannulata is relatively weak, so that the stropharia rugosoannulata becomes a small mushroom seed once and is only cultivated locally in parts of provinces such as Fujian, Jiangxi, Sichuan, Hubei and the like.

As the stropharia rugoso-annulata of Xinxiu and blue sea industries in the bacterial community, a large amount of basic research work needs to be carried out urgently, particularly the difficult problem of strain production which needs to be solved by industrial development, most of the strain research of the current stropharia rugoso-annulata still stays on solid strains, liquid strain research is less, the production application of the liquid strains is almost blank, and the problem is that the industrial development of the stropharia rugoso-annulata needs to be researched.

Disclosure of Invention

the invention provides a liquid culture medium for the industrialized production of stropharia rugoso-annulata, which meets the nutrition required by the growth of stropharia rugoso-annulata hypha and provides a reference for the production of liquid strains of the stropharia rugoso-annulata.

in order to achieve the purpose, the invention provides the following technical scheme: a liquid culture medium for the industrialized production of stropharia rugoso-annulata comprises the following raw materials in proportion: 4-6g/L of soybean meal, 4-6g/L of corn meal, 4-6g/L of bran powder, 25-35g/L of white granulated sugar, 0.5-1g/L of monopotassium phosphate, 0.5-1g/L of magnesium sulfate and 0.3-1g/L of peptone.

Preferably, the composition is prepared from the following raw materials in parts by weight: 6g/L of soybean meal, 6g/L of corn meal, 6g/L of bran powder, 30g/L of white granulated sugar, 1g/L of monopotassium phosphate, 0.6g/L of magnesium sulfate and 0.3g/L of peptone.

preferably, the composition is prepared from the following raw materials in parts by weight: 5g/L of soybean meal, 5g/L of corn meal, 5g/L of bran powder, 35g/L of white granulated sugar, 1g/L of monopotassium phosphate, 0.6g/L of magnesium sulfate and 0.6g/L of peptone.

Preferably, the composition is prepared from the following raw materials in parts by weight: 6g/L of soybean meal, 6g/L of corn meal, 6g/L of bran powder, 30g/L of white granulated sugar, 0.6g/L of monopotassium phosphate, 0.6g/L of magnesium sulfate and 0.6g/L of peptone.

The preparation method of the liquid culture medium for the industrialized production of the stropharia rugoso-annulata comprises the following steps:

s1: respectively grinding soybean powder, corn flour, bran powder, white granulated sugar, potassium dihydrogen phosphate, magnesium sulfate and peptone, and then crushing and sieving by using a 200-mesh sieve with a pore diameter;

s2: accurately weighing the raw materials according to the formula proportion, and putting the raw materials into a barrel to be stirred and dissolved uniformly;

s3: fixing the volume to the volume of the prepared culture medium, and adjusting the pH value to 6.0-6.2;

S4: subpackaging the liquid culture medium with constant volume into a triangular flask with the volume of 1000ml by using a subpackaging device, adding a magnetic stirrer into each bottle with the liquid volume of 600ml, and plugging a cotton plug;

s5: the liquid medium prepared in S4 was sterilized in an autoclave.

Compared with the prior art, the invention has the beneficial effects that: the liquid culture medium for the industrial production of the stropharia rugoso-annulata, provided by the invention, has the advantages of rich raw material sources of the culture medium, rich nutrition of the culture medium and short growth period, and meets the nutritional requirements of the growth of the stropharia rugoso-annulata; the formula is applied to the industrialized liquid spawn production of the stropharia rugoso-annulata, and has the characteristics of rich nutrition, high spawn content of the liquid spawn, pellet forming, uniform size, quick spawn running after the cultivation material is inoculated, and contribution to improving the unit yield and the quality of the stropharia rugoso-annulata.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.