阅读说明：本技术 一种用于防治作物根系病害的微生物菌剂和应用 (Microbial agent for preventing and treating crop root diseases and application ) 是由 方永胜 于 2019-10-22 设计创作，主要内容包括：本发明提供了一种用于防治作物根系病害的微生物菌剂和应用,属于微生物制剂技术领域,所述微生物菌剂包括以下重量份的组分：荧光假单胞菌1～5份、弯曲芽孢杆菌2～6份、枯草芽孢杆菌1～3份、植物乳杆菌1.5～4.5份、抗氧化剂4～8份和稳定剂4～9份。在本发明中,所述微生物菌剂能够防治作物根系病害,能够防治辣椒青枯病、草莓根腐病、大豆根腐病和黄瓜枯萎病。使用本发明提供的微生物菌剂后能够防治作物根系病害,防治效果在85％以上。(The invention provides a microbial agent for preventing and treating crop root diseases and an application thereof, belonging to the technical field of microbial agents, wherein the microbial agent comprises the following components in parts by weight: 1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus curvatus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of antioxidant and 4-9 parts of stabilizer. In the invention, the microbial agent can prevent and treat crop root diseases, and can prevent and treat pepper bacterial wilt, strawberry root rot, soybean root rot and cucumber fusarium wilt. The microbial agent provided by the invention can be used for preventing and treating crop root diseases, and the prevention and treatment effect is over 85%.)

1. A microbial agent for preventing and treating crop root diseases is characterized by comprising the following components in parts by weight:

1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus curvatus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of antioxidant and 4-9 parts of stabilizer.

2. The microbial agent according to claim 1, comprising the following components in parts by weight:

2-4 parts of pseudomonas fluorescens, 3-5 parts of bacillus curvatus, 1.5-2.5 parts of bacillus subtilis, 2-4 parts of lactobacillus plantarum, 5-7 parts of antioxidant and 5-8 parts of stabilizer.

3. The microbial agent according to claim 1, wherein the viable count of Pseudomonas fluorescens is 1X 10¹⁰cfu/g or more.

4. The microbial agent according to claim 1, wherein the number of viable bacteria of Bacillus curvatus is 2X 10⁹cfu/g or more.

5. The microbial inoculant according to claim 1, wherein the effective viable count of the bacillus subtilis is 1.5 x 10¹⁰cfu/g or more.

6. The microbial agent according to claim 1, wherein the effective viable count of Lactobacillus plantarum is 6 x 10⁸cfu/g or more.

7. The microbial inoculant according to claim 1, wherein the antioxidant comprises one or more of dibutylhydroxytoluene, tert-butylhydroquinone and propyl gallate.

8. The microbial inoculant according to claim 1, wherein the stabilizer comprises one or more of malic acid, citric acid, tartaric acid and sorbic acid.

9. The application of the microbial agent as claimed in any one of claims 1 to 8 in controlling crop root diseases.

10. The use of claim 9, wherein the crop root disease comprises pepper bacterial wilt, strawberry root rot, soybean root rot, or cucumber wilt.

Technical Field

The invention belongs to the technical field of microbial preparations, and particularly relates to a microbial preparation for preventing and treating crop root diseases and an application thereof.

Background

Plant diseases refer to the phenomenon that plants generate a series of pathological changes in form, physiology and biochemistry under the influence of biological or non-biological factors, and the normal growth and development processes are blocked, so that the economic benefit of human beings is influenced. The process of a plant's physiological dysfunction, disruption of tissue architecture under the influence of an insult or adverse environmental conditions of a pathogen, a plant disease is an antagonistic symbiosis of a host plant and a pathogen, the occurrence and prevalence of which are the result of the interaction of the host plant and the pathogen. The occurrence of plant diseases often causes serious loss to national economy and people's life.

The control of the diseases of the roots of crops often depends on chemical control, the use of bactericides causes drug resistance of part of pathogenic bacteria, and the use amount of the bactericides has to be increased, which inevitably causes environmental pollution and pesticide residues of agricultural products.

Disclosure of Invention

In view of the above, the present invention aims to provide a microbial agent for preventing and treating crop root diseases and an application thereof.

In order to achieve the above purpose, the invention provides the following technical scheme:

the invention provides a microbial agent for preventing and treating crop root diseases, which comprises the following components in parts by weight:

1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus curvatus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of antioxidant and 4-9 parts of stabilizer.

Preferably, the composition comprises the following components in parts by weight:

2-4 parts of pseudomonas fluorescens, 3-5 parts of bacillus curvatus, 1.5-2.5 parts of bacillus subtilis, 2-4 parts of lactobacillus plantarum, 5-7 parts of antioxidant and 5-8 parts of stabilizer.

Preferably, the effective viable count of the pseudomonas fluorescens is 1 × 10¹⁰cfu/g or more.

Preferably, the number of effective viable bacteria of the Bacillus flexus is 2 x 10⁹cfu/g or more.

Preferably, the effective viable count of the bacillus subtilis is 1.5 multiplied by 10¹⁰cfu/g or more.

Preferably, the effective viable count of the lactobacillus plantarum is 6 multiplied by 10⁸cfu/g or more.

Preferably, the antioxidant comprises one or more of dibutyl hydroxy toluene, tert-butyl hydroquinone and propyl gallate.

Preferably, the stabilizer comprises one or more of malic acid, citric acid, tartaric acid and sorbic acid.

The invention also provides application of the microbial agent in the technical scheme in prevention and treatment of crop root diseases.

Preferably, the crop root diseases comprise pepper bacterial wilt, strawberry root rot, soybean root rot or cucumber fusarium wilt.

The invention provides a microbial agent for preventing and treating crop root diseases and an application thereof, wherein the microbial agent comprises the following components in parts by weight: 1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus curvatus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of antioxidant and 4-9 parts of stabilizer. In the invention, the microbial agent can prevent and treat crop root diseases, and can prevent and treat pepper bacterial wilt, strawberry root rot, soybean root rot and cucumber fusarium wilt. The microbial agent provided by the invention can be used for preventing and treating crop root diseases, and the prevention and treatment effect is over 85%.

Detailed Description

The invention provides a microbial agent for preventing and treating crop root diseases, which comprises the following components in parts by weight: 1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus curvatus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of antioxidant and 4-9 parts of stabilizer.

The microbial agent provided by the invention comprises 1-5 parts of pseudomonas fluorescens, and preferably 2-4 parts. In the present invention, the number of viable bacteria of the Pseudomonas fluorescens is preferably 1X 10¹⁰cfu/g or more. The source of the pseudomonas fluorescens is not specially limited by the invention, and the pseudomonas fluorescens can be prepared by adopting conventional strains, the preparation method of the pseudomonas fluorescens is not specially limited by the invention, and the effective viable count of the pseudomonas fluorescens is preferably 1 multiplied by 10 by adopting the conventional preparation method¹⁰The cfu/g is more than that.

The microbial agent provided by the invention comprises 2-6 parts by weight of bacillus curvatus, preferably 3-5 parts by weight of bacillus curvatus. In the invention, the effective viable count of the curvularia is 2 multiplied by 10⁹cfu/g or more. The source of the campylobacter is not specially limited, the campylobacter can be prepared by adopting conventional strains, the preparation method of the campylobacter is not specially limited, and the effective viable count of the campylobacter is preferably 2 multiplied by 10 by adopting the conventional preparation method⁹The cfu/g is more than that.

The microbial agent provided by the invention comprises 1-3 parts by weight of bacillus subtilis, and preferably 1.5-2.5 parts by weight of bacillus subtilis. In the invention, the effective viable count of the bacillus subtilis is 1.5 multiplied by 10¹⁰cfu/g or more. The invention has no special source to the bacillus subtilisThe preparation method of the bacillus subtilis is not particularly limited, and the effective viable count of the bacillus subtilis is preferably 1.5 multiplied by 10 by adopting the conventional preparation method¹⁰The cfu/g is more than that.

The microbial agent provided by the invention comprises 1.5-4.5 parts by weight of lactobacillus plantarum, and preferably 2-4 parts by weight of lactobacillus plantarum. In the invention, the effective viable count of the lactobacillus plantarum is 6 multiplied by 10⁸cfu/g or more. The source of the lactobacillus plantarum is not particularly limited, the conventional strains can be adopted, the preparation method of the lactobacillus plantarum is not particularly limited, and the conventional preparation method is adopted to ensure that the effective viable count of the lactobacillus plantarum is preferably 6 multiplied by 10⁸The cfu/g is more than that.

The microbial agent provided by the invention comprises 4-8 parts by weight of antioxidant, preferably 5-7 parts by weight. In the present invention, the antioxidant preferably comprises one or more of dibutyl hydroxy toluene, tert-butyl hydroquinone and propyl gallate, and the source of the antioxidant is not particularly limited in the present invention, and conventional commercial products can be adopted. In the invention, the antioxidant improves the inoxidizability of the microbial agent, prolongs the service life of the microbial agent and maintains the drug effect of the microbial agent.

The microbial agent provided by the invention comprises 4-9 parts of a stabilizer, preferably 5-8 parts. In the present invention, the stabilizer preferably includes one or more of malic acid, citric acid, tartaric acid and sorbic acid. The source of the stabilizer is not particularly limited in the present invention, and a conventional commercially available product may be used. In the present invention, the stabilizer increases the stabilizing property of the microbial agent.

In the present invention, the method for preparing the microbial agent is not particularly limited, and it is preferable to mix the respective components.

The application method of the microbial agent is not particularly limited, the microbial agent is preferably applied by root irrigation and irrigation, and the application amount is preferably 10-30 g/mu, and more preferably 15-25 g/mu.

The invention also provides application of the microbial agent in the technical scheme in prevention and treatment of crop root diseases. In the present invention, the crop root diseases preferably include pepper bacterial wilt, strawberry root rot, soybean root rot or cucumber fusarium wilt.

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.