阅读说明：本技术 对虾用保肝复方中草药液体制剂及其制备方法 (Liver-protecting compound Chinese herbal medicine liquid preparation for prawns and preparation method thereof ) 是由 胡庭俊 安慧青 韦英益 施君 于美玲 赵永贞 于 2019-10-24 设计创作，主要内容包括：本发明公开了一种对虾用保肝复方中草药,主要由龙胆草、茵陈、柴胡、栀子、蒲公英组成。据此,发明人还开发了相应液体制剂及其制备方法。本发明来源于纯天然药物,经合理配比获得,具有低残留、低耐药、防治效果良好且无毒副作用等优点,对于对虾肝胰腺可以进行有效保护。试验证明,在对虾‘转肝期’时使用本发明药物保肝护肝,可以促使对虾‘转肝期’顺利进行。(The invention discloses a liver-protecting compound Chinese herbal medicine for prawns, which mainly comprises gentian, oriental wormwood, radix bupleuri, gardenia and dandelion. Accordingly, the inventors have also developed a corresponding liquid formulation and a method for preparing the same. The invention is derived from pure natural medicines, is obtained by reasonable proportioning, has the advantages of low residue, low drug resistance, good prevention and treatment effect, no toxic or side effect and the like, and can effectively protect the hepatopancreas of prawns. Experiments prove that the medicine can be used for protecting the liver when the liver-turning period of the prawn is over, and can promote the liver-turning period of the prawn to be smoothly carried out.)

1. A liver-protecting compound Chinese herbal medicine for prawns is characterized by mainly comprising gentian, oriental wormwood, radix bupleuri, gardenia and dandelion.

2. The liquid preparation of the liver-protecting compound Chinese herbal medicine for the prawns, which is disclosed by claim 1.

3. The liquid preparation according to claim 2, which is characterized by being mainly prepared from the following raw medicinal materials in parts by weight: 10-20 parts of gentian, 10-20 parts of oriental wormwood, 10-20 parts of radix bupleuri, 10-20 parts of cape jasmine and 10-20 parts of dandelion.

4. A process for preparing a liquid preparation according to claim 3, wherein the raw material herbs are pulverized and ground into powder, extracted by water extraction and alcohol precipitation, and then concentrated by rotary evaporation.

5. The method for producing a liquid preparation according to claim 4, characterized by comprising the steps of: weighing the raw medicinal materials according to the formula, adding water according to the mass-volume ratio of 1:15, soaking for 0.5h, then carrying out water bath at 100 ℃ for 2h, and filtering; adding water into the filter residue according to the mass-to-volume ratio of 1:15, continuing the water bath for 1.5h, and filtering; mixing the two filtrates, and centrifuging at 3000r/min for 10 min; concentrating the supernatant in a rotary evaporator, adding 95 vol% ethanol solution to make the volume concentration of ethanol in the mixed solution reach 75%, standing for 24h, filtering the mixed solution, and recovering ethanol by rotary evaporation until the concentration of the liquid medicine is 0.5 g/mL; sterilizing at 115 deg.C under high pressure for 15min, and storing at-20 deg.C.

Technical Field

The invention belongs to the technical field of prawn medicines, and particularly relates to a liver-protecting compound Chinese herbal medicine liquid preparation for prawns and a preparation method thereof.

Background

The penaeus vannamei boone is one of three cultured shrimp varieties in the world at present, and the survival temperature span is large; the salinity adaptability is wide; and has the advantages of rapid growth, low nutrient requirement, high meat yield, rich nutrition and the like, and the global feeding range is gradually expanded in recent years. Since China has been breeding penaeus vannamei boone on a large scale from 1998, the penaeus vannamei boone industry is flourishing. However, the hepatopancreas infectious disease of the prawns has been shown to have an outbreak trend in the world in the year, which leads to the death of the prawns in large quantities.

The liver and pancreas are one of the important organs in the growth and development and immune function of prawn. Research shows that the hepatopancreas reflects the nutritional state of prawn body and is the material metabolism center of prawn due to the special physiological function of the hepatopancreas of invertebrates; besides, the hepatopancreas can also respond to various stress responses of environmental causes to shrimp bodies, such as low-temperature stress, hypotonic stress, low-oxygen stress, ammonia nitrogen stress and the like. The growth process of the prawns has a very important period, namely a liver turning period, if the prawns do not pass through the period smoothly, the weight of the feed is not increased if the prawns are light, and the prawns die if the prawns are heavy, so that huge economic loss is brought to the breeding industry.

With the expansion of the prawn breeding industry, various environmental changes and hepatopancreatic infectious diseases cause huge economic losses to the prawn industry. Once the hepatopancreatic disease of the prawns occurs, the prawns in the whole pond die after a few days, almost no treatment room exists, and farmers are forced to clean the pond. Therefore, the liver and pancreas infectious diseases of the prawns are mainly prevented, and the prawns are helped to successfully pass through the liver turning period by taking the proper medicine orally, so that the immunity function of the prawns is complete and the immunity is increased. Currently, most farmers are mainly fed with antibiotics, however, the use of antibiotic therapy, with time, necessarily results in increased dosage and increased feeding time. Therefore, not only can the sensitivity of the prawns to oral antibiotics be reduced, but also the generation of drug-resistant strains and the exceeding of drug residue can be caused.

The Chinese herbal medicine preparation is derived from natural medicines, can be completely metabolized in an animal body, and avoids the problems of medicine residue and environmental pollution; moreover, natural medicines have multiple action targets and are not easy to cause bacteria to generate drug resistance, and the characteristics become the research direction of liver protection medicines for prawns in the future.

Disclosure of Invention

The invention aims to solve the technical problem of providing a pure natural liver-protecting compound Chinese herbal medicine liquid preparation for prawns, which has low residue, low drug resistance, good prevention and treatment effect and no toxic or side effect, and a preparation method thereof.

In order to solve the technical problems, the invention adopts the following technical scheme:

a compound Chinese medicinal composition for protecting liver of prawn is prepared from radix Gentianae, herba Artemisiae Scopariae, bupleuri radix, fructus Gardeniae, and herba Taraxaci.

The liquid preparation of the liver-protecting compound Chinese herbal medicine for the prawns.

The liquid preparation is mainly prepared from the following raw medicinal materials in parts by weight: 10-20 parts of gentian, 10-20 parts of oriental wormwood, 10-20 parts of radix bupleuri, 10-20 parts of cape jasmine and 10-20 parts of dandelion.

The preparation method of the liquid preparation comprises pulverizing the raw materials, grinding into powder, extracting with water extraction and ethanol precipitation, and concentrating by rotary evaporation.

The preparation method of the liquid preparation comprises the following steps: weighing the raw medicinal materials according to the formula, adding water according to the mass-volume ratio of 1:15, soaking for 0.5h, then carrying out water bath at 100 ℃ for 2h, and filtering; adding water into the filter residue according to the mass-to-volume ratio of 1:15, continuing the water bath for 1.5h, and filtering; mixing the two filtrates, and centrifuging at 3000r/min for 10 min; concentrating the supernatant in a rotary evaporator, adding 95 vol% ethanol solution to make the volume concentration of ethanol in the mixed solution reach 75%, standing for 24h, filtering the mixed solution, and recovering ethanol by rotary evaporation until the concentration of the liquid medicine is 0.5 g/mL; sterilizing at 115 deg.C under high pressure for 15min, and storing at-20 deg.C.

Aiming at the problems of the existing prawn hepatopancreatic infectious disease antibiotic prevention and treatment, the inventor develops a liver-protecting compound Chinese herbal medicine for prawns, which mainly comprises gentian, oriental wormwood, radix bupleuri, gardenia and dandelion. Accordingly, the inventors have also developed a corresponding liquid formulation and a method for preparing the same. The invention is derived from pure natural medicines, is obtained by reasonable proportioning, has the advantages of low residue, low drug resistance, good prevention and treatment effect, no toxic or side effect and the like, and can effectively protect the hepatopancreas of prawns.

The prescription analysis is as follows: the radix Gentianae is dried root and rhizome of Gentiana striolata, Gentiana scabra Bunge, Gentiana trifoliata Sieb or Gentiana rigescens Sieb of Gentianaceae; bitter and cold in nature; it enters liver and gallbladder meridians; has effects in clearing away heat, eliminating dampness, and purging pathogenic fire of liver and gallbladder; it is mainly used for jaundice due to damp-heat, and conjunctival congestion due to liver fire. Herba Artemisiae Scopariae is dry aerial part of herba Artemisiae Scopariae or herba Artemisiae Scopariae of Compositae; bitter, pungent and slightly cold in nature; spleen, stomach, liver and gallbladder meridians entered; has effects in clearing away heat and dampness, and removing liver heat and jaundice; it is mainly used for treating jaundice, oliguria, damp-warm and damp-dampness, etc. Bupleuri radix is dried root of Bupleurum scorzonerifolium or Bupleurum scorzonerifolium of Umbelliferae; pungent, bitter and slightly cold in nature; entering liver, gallbladder and lung meridians; has effects in dispelling pathogenic heat, dispersing stagnated liver qi, relieving qi stagnation, and invigorating yang; it is mainly used for treating common cold and fever. Fructus Gardeniae is dried mature fruit of Gardenia jasminoides Ellis of Rubiaceae; bitter and cold in nature; heart, lung and triple energizer meridians entered; has the functions of purging fire, relieving restlessness, clearing heat, promoting diuresis, cooling blood, removing toxic substances, and relieving swelling and pain by external application; it is mainly used for febrile diseases, vexation, damp-heat jaundice, etc. The herba Taraxaci is dried whole plant of Taraxacum officinale Kitag of Compositae, Taraxacum sinicum Kitag or plants of the same genus; bitter, sweet and cold in nature; entering liver and stomach meridians; has effects in clearing away heat and toxic materials, relieving swelling, dispersing pathogen accumulation, and inducing diuresis for treating stranguria; it is mainly used for treating sore, pyogenic infection, jaundice due to damp-heat, etc.

Experiments prove that the medicine can be used for protecting the liver when the liver-turning period of the prawn is over, and can promote the liver-turning period of the prawn to be smoothly carried out. According to statistics, the domestic prawn yield is 70 ten thousand tons at present, if the prawn yield is 1.0 percent, 0.7 ten thousand tons of prawns are applied every year, and the annual demand is 70 tons based on the average dosage of 1kg of prawns per ton. The product is calculated according to the market price of 150 yuan per kilogram, the annual sales amount reaches 1050 ten thousand yuan, and the pure profit is calculated according to 30 percent and is 315 ten thousand yuan. Therefore, the invention has wide market prospect.

Detailed Description

The liquid preparation of the invention has the usage that: sucking the liquid preparation with corresponding volume according to the medicine-containing proportion, uniformly spraying the liquid preparation on basic ration with corresponding mass by using a sprayer, uniformly mixing to ensure that the liquid preparation is fully absorbed by the feed, and drying in the shade for 24 hours for later use (the unit is mL/kg). Feeding at 3% of body weight daily for 7 days.