阅读说明：本技术 可支持芯片倾斜的多通道孵育装置及试样制备设备 (It can support the inclined multichannel incubating device of chip and sample Preparation equipment ) 是由 廖政 潘颖 唐勇 于 2019-09-20 设计创作，主要内容包括：本发明公开了一种可支持芯片倾斜的多通道孵育装置,其包括有多个孵育组件,各个孵育组件均包括：支架；通过转轴可转动地连接在支架上的转动块,转动块上设有用于放置微流控芯片的平台和用于加热微流控芯片的电加热件,转轴的轴向与平台的第一端至第二端的方向相垂直、且与平台所在的平面相平行,转轴连接在转动块上远离第一端的位置；带动转动块沿转轴转动的驱动组件；位于支架上的挤压件,挤压件位于平台的第一端的转动路径上,转动块由水平位置转动至第一位置时,平台处于第一端高于第二端的倾斜状态、且挤压件能够与微流控芯片的清洗液泡相抵并挤破清洗液泡。该装置能够同步注入清洗液并可以调控液体在微流控芯片内的流动速度。(The invention discloses one kind can support the inclined multichannel incubating device of chip comprising has multiple incubation components, each incubation component includes: bracket；The rotating block being rotatably connected to by shaft on bracket, rotating block is equipped with the electric heating part for placing the platform of micro-fluidic chip and for heating micro-fluidic chip, the direction of first end to the second end of the axial direction and platform of shaft it is perpendicular and with the plane parallel where platform, shaft be connected on rotating block far from first end position；The driving assembly for driving rotating block to rotate along shaft；Extrusion on bracket, extrusion is located on the rotation path of the first end of platform, when rotating block turns to first position by horizontal position, platform is in first end and can offset with the cleaning vacuole of micro-fluidic chip higher than the heeling condition and extrusion of second end and rack cleaning vacuole.The device simultaneous implantation cleaning solution and can regulate and control flowing velocity of the liquid in micro-fluidic chip.)

1. one kind can support the inclined multichannel incubating device of chip, it is suitable for that micro-fluidic chip (2) is heated and kept the temperature It is incubated for, the micro-fluidic chip (2) is provided with the cleaning vacuole (21) for being wrapped in cleaning solution, which is characterized in that described to support The inclined multichannel incubating device of chip includes multiple incubation components (1), and each incubation component (1) includes:

Bracket (11)；

It is rotatably connected to the rotating block (13) on the bracket (11) by shaft (12), is arranged on the rotating block (13) There are the platform (131) for placing the micro-fluidic chip (2) and the electric heating part for heating the micro-fluidic chip (2), The direction of first end to the second end of the axial direction and platform (131) of the shaft (12) it is perpendicular and with the platform (131) plane parallel where, the shaft (12) are connected to the position on the rotating block (13) far from the first end；

The driving assembly for driving the rotating block (13) to rotate along the shaft (12)；

Extrusion (14) on the bracket (11), the extrusion (14) are located at the first end of the platform (131) On rotation path, when the rotating block (13) turns to first position by horizontal position, the platform (131) is in described first End is higher than the heeling condition of the second end and the extrusion (14) can offset with the cleaning vacuole (21) and rack institute State cleaning vacuole (21).

2. can support the inclined multichannel incubating device of chip as described in claim 1, which is characterized in that the driving component Include the power device (15) being fixedly connected with the bracket (11) and connect the power device (15) and the rotating block (13) gear drive.

3. can support the inclined multichannel incubating device of chip as claimed in claim 2, which is characterized in that the gear drive Mechanism includes the driving gear coaxially connected with the power output shaft of the power device (15) and consolidates with the rotating block (13) Surely the center of circle of the sector rack (16) connected, the sector rack (16) is located on the axis of the shaft (12).

4. can support the inclined multichannel incubating device of chip as described in claim 1, which is characterized in that the driving component It include the power device (15) being rotatably connected with the bracket (11), same with the power output shaft of the power device (15) The lead screw (17) of axis connection and the nut block (132) being rotatably connected with the rotating block (13) are set on the nut block (132) It is equipped with the rotary centerline of the threaded hole penetrated for the lead screw (17), the nut block (132) and the power device (15) It is parallel with the rotary centerline of the shaft (12).

5. can support the inclined multichannel incubating device of chip as described in claim 1, which is characterized in that the bracket (11) Upper setting sliding rail, the extending direction of the sliding rail cunning consistent and described with the rotation path of first end of the platform (131) The distance of each position of rail to the shaft (12) is consistent, and the extrusion (14) is slidably attached at the sliding rail On.

6. can support the inclined multichannel incubating device of chip as described in claim 1, which is characterized in that the rotating block (13) be provided with the cavity for accommodating the micro-fluidic chip (2) on, the bottom wall of the cavity formed the platform (131) and The first end of the platform (131) extends the opening for the cavity, and all side walls of the cavity are equipped with electric heating film.

7. can support the inclined multichannel incubating device of chip as described in claim 1, which is characterized in that each electricity adds Warmware be electrically connected with temperature sensor and with the temperature control equipment for adjusting temperature.

8. can support the inclined multichannel incubating device of chip as described in claim 1, which is characterized in that further include being used for Control the control device that each power device (15) is integrally connected.

9. a set of sample Preparation equipment, include micro-fluidic chip (2) for react etc. for sample preparation process and The inclined multichannel incubating device of chip is supported in what is heated for the micro-fluidic chip (2), which is characterized in that described to prop up Holding the inclined multichannel incubating device of chip is to support the inclined multichannel of chip to incubate as claim 1-8 is described in any item Educate device.

10. sample Preparation equipment as claimed in claim 9, which is characterized in that on the chip body of the micro-fluidic chip (2) It is provided with the fluid channel in enclosed construction for flowing for sample, the fluid channel includes the sample-adding storehouse being sequentially communicated (221), reaction warehouse (222), delay path (223), capture storehouse (224) and waste liquid storehouse (225), and the fluid channel is additionally provided with The venthole being connected to ambient atmosphere, the delay path (223) are set as multiple hairs being arranged side by side and successively head and the tail are connected to Capillary structure；Sample-adding storehouse (221) is provided with the reagent ball that solid-like is provided in adding mouth and sample-adding storehouse (221), The reagent ball includes the antibody/antigen for being coated with fluorescent marker and magnetic bead, is arranged on the bottom wall of capture storehouse (224) Have for adsorbing the magnet for occurring to be immunoreacted generated conjugate with the antibody antigen.

Technical field

The present invention relates to technical field of biological, can support the inclined multichannel of chip more specifically to one kind Incubating device and the sample Preparation equipment that the inclined multichannel incubating device of chip can be supported including this.

Background technique

Summary of the invention

The purpose of the present invention is to provide one kind can support the inclined multichannel incubating device of chip, can be to each micro- Fluidic chip carries out heat preservation incubation, and can inject cleaning solution into each micro-fluidic chip simultaneously automatically and can regulate and control liquid Flowing velocity, shortening liquid communication time in micro-fluidic chip.The object of the invention is also to provide one kind can including this Support the sample Preparation equipment of the inclined multichannel incubating device of chip.

One kind provided by the invention can support the inclined multichannel incubating device of chip, be suitable for carrying out micro-fluidic chip Heating and heat preservation are incubated for, and the micro-fluidic chip is provided with the cleaning vacuole for being wrapped in cleaning solution, described that chip is supported to tilt Multichannel incubating device include multiple incubation components, each incubation component includes: bracket；It can be turned by shaft Connect rotating block on the bracket dynamicly, be provided on the rotating block platform for placing the micro-fluidic chip and For heating the electric heating part of the micro-fluidic chip, the first end of the axial direction of the shaft and the platform to the side of second end To it is perpendicular and with the platform where plane parallel, the shaft is connected on the rotating block far from described first The position at end；The driving assembly for driving the rotating block to rotate along the shaft；Extrusion on the bracket, it is described to squeeze Casting die is located on the rotation path of the first end of the platform, when the rotating block turns to first position by horizontal position, institute State platform be in the first end higher than the second end heeling condition and the extrusion can be with the cleaning vacuole phase It supports and racks the cleaning vacuole.

Preferably, the driving component includes the power device being fixedly connected with the bracket and connect the power The gear drive of device and the rotating block.

Preferably, the gear drive includes the driving tooth coaxially connected with the power output shaft of the power device Wheel and the sector rack being fixedly connected with the rotating block, the center of circle of the sector rack is located on the axis of the shaft.

Preferably, the driving component includes that the power device being rotatably connected with the bracket and the power fill The nut block power output shaft set coaxially connected lead screw and be rotatably connected with the rotating block is arranged in the nut block There are the threaded hole penetrated for the lead screw, the rotation of the rotary centerline and the shaft of the nut block and the power device Center line is parallel.

Preferably, sliding rail, the rotation of the extending direction of the sliding rail and the first end of the platform are set on the bracket Path is consistent and the distance of each position to the shaft of the sliding rail is consistent, and the extrusion is slidably attached at On the sliding rail.

Preferably, the cavity for accommodating the micro-fluidic chip, the bottom wall of the cavity are provided on the rotating block The first end for forming the platform and the platform extends opening for the cavity, and the inner wall of the cavity is equipped with electricity Heating film.

Preferably, each electric heating part is electrically connected with temperature sensor and controls with the temperature for adjusting temperature Device.

It preferably, further include having the control device be integrally connected for controlling each power device.

It include for carrying out the micro- of preparation process such as reacting for sample the present invention also provides a set of sample Preparation equipment Fluidic chip and the inclined multichannel incubating device of chip is supported for what is heated for the micro-fluidic chip, feature exists In described that the inclined multichannel incubating device of chip is supported to support the inclined multichannel of chip to be as above described in any item Incubating device.

Preferably, be provided on the chip body of the micro-fluidic chip for for sample flow in enclosed construction Fluid channel, the fluid channel include the sample-adding storehouse being sequentially communicated, reaction warehouse, delay path, capture storehouse and waste liquid storehouse, and described Fluid channel is additionally provided with the venthole being connected to ambient atmosphere, and the delay path is set as multiple and is arranged side by side and successively head and the tail The capillary pipe structure of connection；The sample-adding storehouse is provided with the reagent ball that solid-like is provided in adding mouth and the sample-adding storehouse, institute Stating reagent ball includes the antibody/antigen for being coated with fluorescent marker and magnetic bead, is provided with and is used on the bottom wall in the capture storehouse The magnet for being immunoreacted generated conjugate occurs with the antibody antigen for absorption.

In technical solution provided by the invention, it is clear can to support that the inclined multichannel incubating device of chip is suitable for being provided with band The micro-fluidic chip of washing lotion bubble, this can support that the inclined multichannel incubating device of chip includes multiple incubation components, each to incubate Educating component includes bracket, the shaft being fixed on bracket and the platform for carrying out incubation with heat to micro-fluidic chip.It should Platform is disposed around on the rotating block that shaft may be rotated, and the axial direction of shaft and the first end of platform are to second end Direction it is perpendicular and with the plane parallel where platform.Shaft is connected to the position on rotating block far from first end, then puts down The first end of platform can be rotated around the central axis of shaft along the path perpendicular to plane where platform.Micro-fluidic chip is put Can carry out rotation after setting on platform therewith tilts micro-fluidic chip.The starting point of fluid channel is set to be located at the first of platform End, then the liquid in fluid channel can be flowed in the case where fluid channel is in heeling condition, can be speeded flowing velocity, be shortened liquid flow The logical time；And each rotating block can also it is different according to the reaction duration needed for various sample and carry out the adjustment of tilt angle, Enhance applicability and convenience.Meanwhile it can support the inclined multichannel incubating device of chip is additionally provided with to be fixed on bracket Extrusion, extrusion are located on the rotation path of the first end of platform.First is turned to by horizontal position in the first end of platform When position, the cleaning vacuole for the first end that extrusion can be located at platform with micro-fluidic chip offsets and racks cleaning vacuole, makes Cleaning solution is flowed out and is injected in fluid channel.First position is turned to simultaneously by controlling multiple rotating blocks, may be implemented to same The operation for being automatically injected cleaning solution in multiple micro-fluidic chips of test simultaneously is criticized, it is convenient to save trouble；And realize cleaning solution injection Synchronism so that detection operation can carry out simultaneously, can to avoid influence test consistency.

Detailed description of the invention

In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with It obtains other drawings based on these drawings.

Fig. 1 is the structural schematic diagram for supporting the inclined multichannel incubating device of chip in the embodiment of the present invention；

Fig. 2 is the structural schematic diagram that component is incubated in the embodiment of the present invention；

Fig. 3 be the embodiment of the present invention in platform to first position schematic diagram；

Fig. 4 is the structural schematic diagram of driving assembly in the embodiment of the present invention；

Fig. 5 is the structural schematic diagram of micro-fluidic chip in the embodiment of the present invention；

Fig. 6 is the structural schematic diagram of fluid channel in the embodiment of the present invention.

In Fig. 1-Fig. 6:

It is incubated for component -1, micro-fluidic chip -2, cleaning vacuole -21, sample-adding storehouse -221, reaction warehouse -222, delay path - 223, storehouse -224, waste liquid storehouse -225, place position -23, opening -231, injection channel -24, cabinet -3, bracket -11, shaft-are captured 12, rotating block -13, platform -131, nut block -132, extrusion -14, power device -15, sector rack -16, lead screw -17.

Specific embodiment

To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work Other embodiment belongs to the range that the present invention is protected.

Being designed to provide one kind and can supporting the inclined multichannel incubating device of chip for present embodiment, can Heat preservation incubation carried out to each micro-fluidic chip, and cleaning solution can be injected into each micro-fluidic chip simultaneously automatically and can be with Regulate and control flowing velocity of the liquid in micro-fluidic chip, shorten the liquid communication time.The purpose of present embodiment also resides in A kind of sample Preparation equipment that the inclined multichannel incubating device of chip can be supported including this is provided.

Hereinafter, elaborating in conjunction with attached drawing to embodiment.In addition, embodiments illustrated below is not remembered claim The content of the invention of load plays any restriction effect.In addition, the full content of composition represented by following example is not limited to conduct Necessary to the solution of the invention described in the claims.

Please refer to attached drawing 1-6, one kind provided in this embodiment can support the inclined multichannel incubating device of chip, for for Micro-fluidic chip 2 carries out incubation with heat.In the prior art, as shown in Figure 5 and Figure 6, micro-fluidic chip 2 is provided with fluid channel and packet The cleaning vacuole 21 of cleaning solution is wrapped up in, cleaning vacuole 21 is located at the starting point of fluid channel and is connected to fluid channel, in cleaning solution It can be directly injected into fluid channel after cleaning solution outflow in bubble 21.It is provided in this embodiment to support the inclined multichannel of chip Incubating device includes multiple incubation components, multiple micro-fluidic chips 2 of same a batch test can be carried out synchronous incubation simultaneously, To keep the consistency of test.As shown in Fig. 2, each shaft for being incubated for component and including bracket 11, being fixed on bracket 11 12, the rotating block 13 being rotatably connected with shaft 12, the driving assembly for driving rotating block 13 to rotate.It is arranged on rotating block 13 useful In the platform 131 for carrying out incubation with heat to micro-fluidic chip 2.The platform 131 is rotated around shaft 12, and shaft 12 Plane parallel axial perpendicular with the direction of first end to the second end of platform 131 and where with platform 131, shaft 12 It is connected to the position on rotating block 13 far from first end, then the first end of platform 131 can hang down around the central axis edge of shaft 12 Directly rotated in the path of 131 place plane of platform.Micro-fluidic chip 2 can be turned therewith after being placed on platform 131 It is dynamic that micro-fluidic chip 2 is made to be in heeling condition.The starting point of fluid channel is set to be located at the first end of platform 131, then in fluid channel Liquid can be flowed in the case where fluid channel is in heeling condition, can be speeded the flowing velocity of liquid, be shortened liquid communication fluid channel Time.Micro-fluidic chip 2 can be in heeling condition in cleaning solution flowing, shorten cleaning solution from fluid channel starting point It flow to the circulation duration of end.And each rotating block 13 can also the reaction duration according to needed for different samples it is different, into The adjustment of line tilt angle.In this way, enhancing the applicability and convenience that can support the inclined multichannel incubating device of chip, such as It can be according to the specific inclination of the difference adjustment micro-fluidic chip 2 of duration needed for the duration and sample circulation needed for immune response Angle, to shorten the time for waiting sample to be immunoreacted, accelerate experiment process.

As shown in figure 3, simultaneously, can support the inclined multichannel incubating device of chip is additionally provided with to be fixed on bracket 11 Extrusion 14, extrusion 14 are located on the rotation path of the first end of platform 131.Platform 131 first end by horizontal position When turning to first position, extrusion 14 can be located at 21 phase of cleaning vacuole of the first end of platform 131 with micro-fluidic chip 2 It supports and racks cleaning vacuole 21.So set, racking micro-fluidic chip 2 while being in heeling condition by cleaning vacuole 21 So that cleaning solution is flowed out and is injected in fluid channel, cleaning is then rapidly completed in an inclined state, whole process is realized automatically, convenient It is time saving.And it may be implemented by controlling multiple rotating blocks 13 while turning to first position to the multiple micro-fluidic of same a batch test The operation of cleaning solution is automatically injected in chip 2 simultaneously, it is convenient to save trouble；And the synchronism of cleaning solution injection is realized, so that detection The consistency that operation can be carried out simultaneously, can be tested to avoid influence.Horizontal position is 0 degree of position, and first position can be place Any angular position within the scope of 0-90 degree.

Specifically, driving assembly can be include the power device 15 being fixedly connected with bracket 11 and connect power dress Set the transmission mechanism of 15 Yu rotating block 13.Power device 15 can be motor and hydraulic motor either rotary cylinder, it is preferable that It can be set to motor.Compared to hydraulic motor and rotary cylinder, the volume of motor is more small and exquisite and at low cost, easy connection.Such as Fig. 2 Shown, transmission mechanism can be gear drive, naturally it is also possible to be that other such as chain conveyers or lead screw 17 are driven transmission knot Structure.Gear transmission structure has the characteristics that transmission apart from short, stability is more preferable.Gear drive can be including with power The coaxially connected driving gear of the power output shaft of device 15 and the driven gear or sector rack being fixedly connected with rotating block 13 16.The center of circle of driven gear or sector rack 16 is respectively positioned on the axis of shaft 12.Since platform 131 is micro-fluidic for placing Chip 2, the tilt angle of micro-fluidic chip 2, which is in less than 90 degree ranges, to be advisable, so using sector rack 16 as shown in Figure 3 Have the advantages that structure is simpler, be more convenient for producing.The fan angle of sector rack 16 can carry out specifically according to actual needs Setting is rotated in 0 to 90 degree range with being able to achieve the first end of platform 131.

As shown in figure 4, transmission mechanism still can be driven with lead screw 17, driving assembly includes to be rotatably connected with bracket 11 Power device 15, with the coaxially connected lead screw 17 of power output shaft of power device 15 and be rotatably connected with rotating block 13 Nut block 132, is provided with the threaded hole penetrated for lead screw 17 in nut block 132, in the rotation of nut block 132 and power device 15 Heart line is parallel with the rotary centerline of shaft 12.So set, the power output shaft when power device 15 rotates, lead screw 17 It rotates with it, lead screw 17 and 132 screw-driven of nut block, rotating block 13 is made to rotate with it generation inclination, while power device 15 With nut block 132 can generate rotation, prevent lead screw 17 is stuck.

Extrusion 14 can be pricker and be also possible to the object with pointed structures, or as shown in Fig. 2, extrusion 14 is One crushing block, crushing block have the compressive plane of the first end of object platform 131.When the first end of platform 131 turns to first Position, the compressive plane and cleaning vacuole 21 of crushing block offset and rack cleaning vacuole, at this time the plane phase of compressive plane and platform 131 In parallel.Extrusion 14 can be fixedly connected by screw with bracket 11.It can be set multiple with the different extruding for squeezing angle Part 14 more brings extrusion 14 adapt to each extruding angle that platform 131 is under differing tilt angles according to demand. Alternatively, extrusion 14, which can adjust itself with 11 slidable connection of bracket, adapts to difference relative to the position of bracket 11 Extruding angle.Such as, sliding rail, the extending direction and the rotation path phase one of the first end of platform 131 of sliding rail are set on bracket 11 It causes and the distance of each position to the shaft 12 of sliding rail is consistent, extrusion 14 is slidably attached on sliding rail.Sliding rail can be with It is groove or strip gab on the bracket 11, extrusion 14 is stuck in cunning by the card slot that is located on itself side wall or protrusion The opening of rail.

Micro-fluidic chip 2 is placed on platform 131 and rotates synchronously with platform 131, then micro-fluidic chip 2 can pass through rope Son or rubber band are tied up on rotating block 13, or are fixed on platform 131 by magnetic structure.For adding for micro-fluidic chip 2 The electric heating part of heat, which can be, is laid on 131 lower section of platform, and electric heating part can be electric heating wire and be also possible to electric heating film.Respectively A electric heating part for being incubated for component can be electrically connected with temperature sensor and temperature control equipment, such as temperature controller or temperature relay Device in order to carry out heated at constant temperature to micro-fluidic chip 2, and can specifically set heating temperature.

Alternatively, as shown in Fig. 2, be provided with the cavity for accommodating micro-fluidic chip 2 on rotating block 13, the bottom wall shape of cavity Extend at the first end of above-mentioned platform 131 and the platform 131 for the opening of cavity, so that micro-fluidic chip 2 is placed on platform Vacuole 21 is cleaned when on 131 to be located at except cavity.Micro-fluidic chip 2, which can be inserted in cavity, to be fixed.So set, cavity All side walls can be equipped with electric heating film, carry out comprehensive to micro-fluidic chip 2 while heating.And cavity can be and pass through Die casting and rotating block 13 are integrally formed, and are also possible to as shown in Fig. 2, rotating block 13 is by having reeded pedestal and being connected with pedestal The top cover combination connect forms, and open at one end, the upper cover covering part groove of the length direction of groove form above-mentioned cavity.

In order to realize the synchronous rotation of each rotating block 13 for being incubated for component, each power device 15 for being incubated for component and electricity The global switch for all power devices 15 that are same as being turned on or off is electrically connected between source, and be realize it is each be incubated for component it is only Vertical rotation, is electrically connected with independent switch between each power device 15 and global switch.Alternatively, can be propped up in some embodiments Holding the inclined multichannel incubating device of chip further includes having the control device be integrally connected for controlling each power device 15, such as Single-chip microcontroller or PLC, it is preferable that PLC can be used, so can directly be bought on the market.It is west gate that PLC, which can select model, The PLC of sub- s7-200smart.On this basis, each electric heating part for being incubated for component and temperature sensor can also be with monolithics Machine or PLC are electrically connected, and are realized by circuit control that single-chip microcontroller or PLC are turned on or off to each electric heating part To the computer heating control of micro-fluidic chip 2.

As shown in Figure 1, can support the inclined multichannel incubating device of chip further include have it is each for fixing and accommodating State the cabinet 3 for being incubated for component, each incubations component is arranged side by side, and be provided on cabinet 3 it is opposite with the first end of platform 131 just Opening, protrude into for micro-fluidic chip 2.The input terminal of control device can be set on cabinet 3, can be as shown in Figure 1 Touch button and display screen.

The present embodiment additionally provides a set of sample Preparation equipment, includes for carrying out the preparation process such as reacting for sample Micro-fluidic chip 2 and the inclined multichannel incubating device of chip is supported for what is heated for the micro-fluidic chip 2, this can be supported The inclined multichannel incubating device of chip supports the inclined multichannel incubating device of chip to be as above as described in the examples.Then The set sample Preparation equipment, which has, supports the identical beneficial effect of the inclined multichannel incubating device of chip with above-mentioned, this is beneficial The derivation process of effect and the above-mentioned derivation process for supporting beneficial effect brought by the inclined multichannel incubating device of chip Substantially similar, repeats no more herein.

Meanwhile on the chip body for the micro-fluidic chip 2 in the set sample Preparation equipment including, it is provided with for for sample The fluid channel in enclosed construction of flowing.In the prior art, the reagent being immunoreacted with sample is usually all by changing It learns to do section and chip body is solidified, to be encapsulated in fluid channel 3.When sample injection 3 cocurrent of fluid channel cured region, Sample and reagent are in contact and are immunoreacted.Such mode be easy to cause reagent and sample contacts it is insufficient, immune response It is not easy sufficiently to carry out, the problems such as required sample is more；And reagent and chip body solidify needed for it is at high cost, process is cumbersome, right The structural requirement of fluid channel 3, the material of chip body require and transport requirement is high, causes production difficulty larger.To solve The above problem, in the present embodiment, specifically, as shown in Figure 5 and Figure 6, fluid channel includes the sample-adding storehouse 221 being sequentially communicated, anti- It answers storehouse 222, delay path 223, capture storehouse 224 and waste liquid storehouse 225 and is provided with the venthole being connected to ambient atmosphere.It is loaded storehouse 221 be provided with adding mouth and sample-adding storehouse 221 in be provided with the solid-like reagent ball being immunoreacted with the sample.Reagent ball Include the antibody/antigen for being coated with fluorescent marker and magnetic bead, captures and be provided on the bottom wall in storehouse 224 for absorption and antibody The magnet for being immunoreacted generated conjugate occurs for antigen.

Sample injects in sample-adding storehouse 221 from adding mouth can be such that reagent ball dissolves in sample, making reagent and sample completely mixed It closes, come into full contact with.In this way, reagent ball is built in fluid channel 3, eliminates and liquid has been coated with fluorescence mark in the prior art Remember the antibody/antigen of object and capture antibody/antigen and solidifies the production procedure being integrated with chip body；Reagent ball in solid-like, Encapsulation is easier to compared to liquid to save and guarantee the quality, and is also reduced and is pushed away the material requirement, the life that fill the fluidic chip that declines to single channel It produces to require and transport to save and require.Compared with prior art, the micro-fluidic chip of the present embodiment is so set, reagent can be complete Dissolve in sample and adequately with sample mixed, guarantee the abundant progress of immune response, and reagent can be with sample common stream It moves, can be carried out being immunoreacted during the entire process of flowing.Such mode provided in this embodiment preferably ensure that It is immunoreacted the electrochemical conditions sufficiently occurred.

Then composite sample flows into reaction warehouse 222 from sample-adding storehouse 221, is further mixed well and is immunized Reaction.The flow process of sample and the reaction warehouse 222 individually set up, sample and fluorescent marker can be made to be mixed well, And it is immunoreacted rapidly and comprehensively.

Capture storehouse 224 is provided between reaction warehouse 222 and waste liquid storehouse 225, and the bottom wall for capturing storehouse 224 is provided with magnet. The antibody/antigen as contained by reagent ball has been coated with magnetic bead, then is immunoreacted generated conjugate with it and is also coated with Magnetic bead, magnet can adsorb conjugate.So set, being immunoreacted the conjugate of production when composite sample flows through capture storehouse 224 It can be captured and be gathered in capture storehouse 224 and remaining sample flows into waste liquid storehouse 225.Magnet can be the bottom for being laid on capture storehouse 224 In the whole region of wall.Immune response sufficiently carries out before composite sample incoming seizure storehouse 224, thereafter just incoming seizure storehouse 224, which are substantially all the conjugate generated, can be captured, can guarantee quantity of the catch, flow through capture with sample in the prior art The position of antibody/antigen reacts with fixed capture antibody/antigen and is captured and compares, the knot of this flow-type capture Structure makes conjugate be easy to captured, but also the amount that conjugate flows into waste liquid storehouse 225 substantially reduces, ensure that detection dosage and inspection The correctness of survey is but also required sample dosage reduces, saves sample cost.

It should be noted that the antibody/antigen for being coated with fluorescent marker and magnetic bead belongs to existing production in the prior art Product.

Delay path 223 can be screw type curved structure, V-arrangement curved structure or other shapes of curved structure.It is preferred that Ground, delay path 223 are provided with multiple capillaries, and multiple capillaries are arranged along the direction in reaction warehouse 222 to capture storehouse 224, and Multiple capillaries head and the tail are sequentially communicated, the delay path 223 to form S-shaped structure.So set, sample complications stream can be made It is dynamic slow down sample by time of delay path 223, come duration needed for guaranteeing sample immune response, and it is special to pass through capillary Property make sample flow forward, promote sample mobility, can be to avoid applying additional flowing thrust；And by adjusting chip sheet The tilt angle of body can control the tilt angle of fluid channel 3, so as to adjust the delay path that composite sample flows through detour 223 time allows the time of immune response to carry out practical adjustment according to the different of sample.Both it can guarantee immune response Duration, and be avoided that and waste the excessive time, cause to drag slow sample from the whole process for being injected into detection completion.The micro-fluidic chip 2 and it is above-mentioned support the inclined multichannel incubating device of chip to match, the control of the flowing velocity of liquid can achieve Better effect.

As shown in figure 5, the chip body of micro-fluidic chip 2 can be by having reeded substrate and being connected with substrate Upper cover composition, upper cover cover the fluid channel that groove forms enclosed construction.Adding mouth be respectively positioned on upper lid with venthole and with it is micro- Runner is connected.The place position 23 for placing cleaning vacuole 21 is additionally provided on upper lid, place position 23 is provided with opening 231. The starting point that the groove of fluid channel is formed on substrate is provided with injection channel 24, the first end and reaction warehouse 222 of injection channel 24 It is connected.After substrate is connect with upper cover, opening 231 it is opposite with the second end of injection channel 24 just and connection.When cleaning vacuole After 21 are squeezed and broken, cleaning solution flows into injection channel 24 from opening 234, rinses sample subsequently into reaction warehouse 222, finally flows Enter waste liquid storehouse 223.Cleaning vacuole 21, which can be, to be wrapped in cleaning solution with plastic film and is formed.

It is understood that same or similar part can mutually refer in the various embodiments described above, in some embodiments Unspecified content may refer to the same or similar content in other embodiments.Multiple schemes provided by the invention include The basic scheme of itself does not condition each other independently of each other, but it can also be combined with each other in the absence of conflict, reach It is realized jointly to multiple effects.

The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one The widest scope of cause.