阅读说明：本技术 一种抗结核杆菌的王枣子提取物颗粒剂及制备方法 (A kind of agent of common rabdosia leaf extract particles and preparation method of anti-mycobacterium tuberculosis ) 是由 盛玮 段永波 滕井通 薛建平 张爱民 朱艳芳 于 2019-09-04 设计创作，主要内容包括：本发明公开了一种抗结核杆菌的王枣子提取物颗粒剂及制备方法,其特征在于。本发明所述的颗粒剂由以下重量份的原料制成：王枣子提取物12-18份,药用淀粉58-76份,蔗糖4-8份,羟甲基纤维素钠1-2份,硬脂酸钠1-2份。本发明以王枣子为原料,加入辅料制备成具有抗结核杆菌功效的颗粒剂,充分利用了王枣子的有效成分,制备的王枣子提取物颗粒剂可以预防和治疗结核病,服用方便,而且对王枣子的开发利用具有重要意义。(The agent of common rabdosia leaf extract particles and preparation method the invention discloses a kind of anti-mycobacterium tuberculosis, it is characterised in that.Granule of the present invention is made of raw material from the following weight: 12-18 parts of common rabdosia leaf extract, 58-76 parts of medical starch, 4-8 parts of sucrose, 1-2 parts of sodium cellulose glycolate, 1-2 parts of odium stearate.The present invention is using common rabdosia leaf as raw material, auxiliary material is added and is prepared into the granule with anti-mycobacterium tuberculosis, takes full advantage of the effective component of common rabdosia leaf, the common rabdosia leaf extract particles agent of preparation can prevent and treat tuberculosis, it is convenient to take, and be of great significance to the development and utilization of common rabdosia leaf.)

1. a kind of common rabdosia leaf extract particles agent of anti-mycobacterium tuberculosis, which is characterized in that be made of raw material from the following weight: king 12-18 parts of Chinese date extract, 58-76 parts of medical starch, 4-8 parts of sucrose, 1-2 parts of sodium cellulose glycolate, odium stearate 1-2 Part.

2. the common rabdosia leaf extract particles agent of anti-mycobacterium tuberculosis as described in claim 1, it is characterised in that: the common rabdosia leaf mentions Object is taken to be prepared by the following method to obtain:

(1) common rabdosia leaf herb is cleaned up, crushed after being dried, crosses the sieve of 80 mesh, obtains common rabdosia leaf powder；

(2) (1) resulting common rabdosia leaf powder and digestion agent ratio are fed intake for 1: 5-30 (w/w) and extracts extraction, extraction temperature 40-100 DEG C, extraction time -10 hours 10 minutes, when extraction extracts, intermittent stirring raw material filtered extracting solution, and collection obtains filtrate and filter Slag；Filter residue is repeated into extraction 1-2 times, repeats to filter, finally merges all filtrates；

The composition and proportion of digestion agent used are in parts by weight are as follows: -100 parts of 60-95% alcohol 95, emulsifier 0-5 Part；

(3) (2) resulting common rabdosia leaf extracting solution is concentrated into the 1/5- of original volume under the conditions of vacuum degree≤0.08MPa, 65 DEG C 1/10；

(4) in (3) resulting common rabdosia leaf concentrated extracting solution, hydroxypropyl-is added by 0.01-0.05 times of common rabdosia leaf herb quality Beta-cyclodextrin, it is spray-dried to obtain common rabdosia leaf extract after being uniformly dispersed with blender.

3. the preparation method of the common rabdosia leaf extract particles agent of anti-mycobacterium tuberculosis as described in claim 1, it is characterised in that packet It includes following steps: first weighing raw material by above-mentioned parts by weight；Then common rabdosia leaf extract, medical starch, sucrose, methylol is fine Tie up plain sodium, odium stearate is uniformly mixed, dry granulation to get.

Technical field

The present invention relates to the common rabdosia leaf extract particles agent of natural medicine technical field more particularly to a kind of anti-mycobacterium tuberculosis And preparation method.

Background technique

Common rabdosia leaf (Isodon amethystoides(Benth) Cy Wu et Hsuan) it is that Labiatae Rabdosia is planted Object, root, stem and leaf are all medicinal part, are the Chinese traditional herbs of Xiuzhou City, Anhui Province local speciality, according to " Suzhou will ", " Su County will " Record: " common rabdosia leaf is grown in Su County the north and accords with from, the valley Jia Gou, Cao Cun, Huang Zang one with mountain area, is wild medical and edible dual purpose plant, tool There are antibacterial anti-inflammatory, removing toxic substances of bringing down a fever, relieving cough and asthma, whole intestines stomach invigorating and other effects." in common rabdosia leaf containing terpene, alkaloid, flavonoids, A variety of natural active matters such as polysaccharide and volatile oil, wherein ter penoids mainly have wangzaozin A, B prime and C prime, Mao Ye Alcohol, oleanolic acid and the street Bu Gu alcohol etc..Research shows that common rabdosia leaf has antibacterial anti-inflammatory, clearing heat and detoxicating, anti-aging, antifatigue, anti- Tumour, health-care sobering, improves the effects of autoimmunity at protect liver stomach function regulating, is known as " natural plants antibiotic ".Currently, king's jujube The exploitation of son is very few, civil to be directly boiled into soup with common rabdosia leaf and drink, and the effective component of common rabdosia leaf is researched and developed using insufficient It is a kind of to treat and prevent common rabdosia leaf extract particles agent lungy and have great importance.

Tuberculosis (tuberculosis, TB) be by mycobacterium tuberculosis (mycobacterium tuberculosis, MTB a kind of chronic lethal disease caused by) is to endanger human health and lead to the great communicable disease of human death.Its Lethality is only second to AIDS (acquiredimmunodeficiency syndrome, AIDS).It is big due to antituberculotic Amount leads to multi-drug resistance tuberculosis (multidrug- using the mutation with treating tuberculosis action site Resistanttuberculosis, MDR-TB) it continuously emerges.There are 10,400,000 new cases in world wides in 2016, China is Global 22 TB prevalence one of the countries with the most serious ... are sent out according to the 5th tuberculosis epidemiology investigation estimation year of the whole nation in 2010 Patient's number is the 14.3% of 1,300,000, Zhan Quanqiu neopathy number, is world's second largest tuberculosis high burden country.Tuberculosis at present The treatment cycle of disease is longer, and patient's compliance is poor.One line antituberculotic and two wires antituberculotic clinically make extensively With, there is Multiple drug-resistan Tubercle bacillus, cannot reach effectively control tuberculosis.China's Chinese medicine it is resourceful, when treatment, is paid attention to whole Body collaboration, and toxic side effect is small and the features such as be not likely to produce drug resistance and advantage, so that Antituberculous Chinese Traditional Medicine is increasingly serious Increasingly brighter application prospect has been shown in terms of tuberculosis therapy.

Summary of the invention

The object of the present invention is to provide a kind of drugs of new anti-mycobacterium tuberculosis, specifically, the present invention provides one kind The common rabdosia leaf extract particles agent of anti-mycobacterium tuberculosis and preparation method.

To achieve the goals above, the present invention adopts the following technical scheme:

A kind of common rabdosia leaf extract particles agent of anti-mycobacterium tuberculosis, which is characterized in that be made of raw material from the following weight: king's jujube 12-18 parts of seed extract, 58-76 parts of medical starch, 4-8 parts of sucrose, 1-2 parts of sodium cellulose glycolate, 1-2 parts of odium stearate.

The common rabdosia leaf extract is prepared by the following method to obtain:

(1) common rabdosia leaf herb is cleaned up, crushed after being dried, crosses the sieve of 80 mesh, obtains common rabdosia leaf powder；

(2) (1) resulting common rabdosia leaf powder and digestion agent ratio are fed intake for 1: 5-30 (w/w) and extracts extraction, extraction temperature 40-100 DEG C, extraction time -10 hours 10 minutes, when extraction extracts, intermittent stirring raw material filtered extracting solution, and collection obtains filtrate and filter Slag；Filter residue is repeated into extraction 1-2 times, repeats to filter, finally merges all filtrates；

The composition and proportion of digestion agent used are in parts by weight are as follows: -100 parts of 60-95% alcohol 95, emulsifier 0-5 Part；

(3) (2) resulting common rabdosia leaf extracting solution is concentrated into the 1/5- of original volume under the conditions of vacuum degree≤0.08MPa, 65 DEG C 1/10；

(4) in (3) resulting common rabdosia leaf concentrated extracting solution, hydroxypropyl-is added by 0.01-0.05 times of common rabdosia leaf herb quality Beta-cyclodextrin, it is spray-dried to obtain common rabdosia leaf extract after being uniformly dispersed with blender.

The preparation method of the common rabdosia leaf extract particles agent of the anti-mycobacterium tuberculosis, comprising the following steps: first by above-mentioned Parts by weight weigh raw material；Then common rabdosia leaf extract, medical starch, sucrose, sodium cellulose glycolate, odium stearate are mixed Uniformly, dry granulation to get.

The effective component of common rabdosia leaf extract is mainly terpene, and terpenoid has strong-hydrophobicity, is unfavorable for absorbing, Bioavilability is lower, therefore hydroxypropyl-β-cyclodextrin is added in common rabdosia leaf extract, makes terpenoid and hydroxy propyl-Beta- Cyclodextrin forms inclusion compound, improves the solubility property of terpenoid, adjusts rate of release, improves the stabilization of common rabdosia leaf extract Property and bioactivity.

The present invention is added auxiliary material and is prepared into the granule with anti-mycobacterium tuberculosis, sufficiently benefit using common rabdosia leaf as raw material Tuberculosis can be prevented and treated with the common rabdosia leaf extract particles agent of the effective component of common rabdosia leaf, preparation, it is convenient to take, and And it is of great significance to the development and utilization of common rabdosia leaf.

Figure of description

Fig. 1 is bactericidal effect experiment photo of the common rabdosia leaf extract particles agent to mycobacteria H37Ra.

Specific embodiment