阅读说明：本技术 一种用于防治松材线虫病的防倒吸注干剂 (Suck-back-preventing dry injection for preventing and treating pine wood nematode disease ) 是由 吴松青 张飞萍 郭雅洁 林志艺 于 2021-08-31 设计创作，主要内容包括：本发明公开了一种用于防控松材线虫病的防倒吸注干剂,其中包含对松材线虫具有高效杀虫活性的1%～12%氟吡菌酰胺和78%～99%乙酸乙酯与水混配防倒吸溶剂。此外,还包含有0%～11%乳化剂,0%～11%稳定剂和0%～11%渗透剂。该注干剂以树干注药法施用,不受松树油脂分泌产生的压强影响,能在任何季节将药物有效地输送到树干中,起到预防松材线虫病的作用。(The invention discloses a suck-back-preventing dry injection for preventing and controlling pine wood nematode diseases, which comprises a suck-back-preventing solvent prepared by mixing 1-12% of fluopyram and 78-99% of ethyl acetate with water, wherein the fluopyram and the ethyl acetate have high insecticidal activity on the pine wood nematodes. In addition, the emulsion also comprises 0-11% of an emulsifier, 0-11% of a stabilizer and 0-11% of a penetrating agent. The trunk injection agent is applied by a trunk injection method, is not influenced by pressure generated by pine oil secretion, can effectively deliver the medicine to the trunk in any season, and plays a role in preventing the pine wood nematode disease.)

1. The suck-back-preventing dry injection for preventing and treating the pine wilt disease is characterized by comprising the following raw material components in percentage by mass: 1% -12% of fluopyram, 78% -99% of ethyl acetate and water are mixed to form an anti-suck-back solvent; mixing ethyl acetate with water to prevent ethyl acetate in the suck-back solvent: the water mass ratio is 4: 1-1: 0.

2. The suck-back-preventing dry injection for preventing and treating the pine wilt disease according to claim 1, characterized in that: the material comprises the following raw material components in percentage by mass: 1% -12% of fluopyram, 78% -99% of ethyl acetate and water are mixed to form an anti-suck-back solvent, 0% -11% of an emulsifier, 0% -11% of a stabilizer and 0% -11% of a penetrating agent.

3. The suck-back-preventing dry injection for preventing and treating the pine wilt disease according to claim 2, characterized in that: the emulsifier comprises one or more of diethylene glycol monobutyl ether, sodium dodecyl benzene sulfonate and alkylphenol polyoxyethylene.

4. The suck-back-preventing dry injection for preventing and treating the pine wilt disease according to claim 2, characterized in that: the stabilizer comprises one or more of phosphorous acid, alkyl phosphite ester, epoxidized soybean oil and epichlorohydrin.

5. The suck-back-preventing dry injection for preventing and treating the pine wilt disease according to claim 2, characterized in that: the penetrating agent comprises one or more of azone and fatty alcohol-polyoxyethylene ether.

6. The suck-back-preventing dry injection for preventing and treating the pine wilt disease is characterized by comprising the following raw material components in percentage by mass: 8% of fluopyram, 77.4% of ethyl acetate, 8.6% of water, 2% of diethylene glycol monobutyl ether, 2% of epichlorohydrin and 2% of fatty alcohol-polyoxyethylene ether.

7. The suck-back-preventing dry injection for preventing and treating the pine wilt disease according to claim 1, characterized in that: is used for injecting the host plants with the tree diameter of more than 5 cm.

8. The suck-back-preventing dry injection for preventing and treating the pine wilt disease according to claim 7, characterized in that: the host plant is a Pinus host plant, including but not limited to Pinus massoniana (A), (B), (C) and (C)Pinus massoniana) "Black pine" (a)P. thunbergii) Xue Song (Chinese white pine)Cedrus deodara) Red pine (Pinus densiflora)P. densiflora) Larch, larch (Larix gmelini) Yunnan pine (1)P. yunnanensis) One kind of (1).

9. The suck-back-preventing dry injection for preventing and treating the pine wilt disease according to claim 1, characterized in that: the tree trunk is applied by a trunk injection method, and the aperture of an injection hole is 1-5 mm during trunk injection.

Technical Field

The invention belongs to the technical field of pesticide compounding, and particularly relates to a suck-back prevention dry injection for preventing and treating pine wood nematode disease.

Background

The pine wood nematode disease is caused by pine wood nematode (1)Bursaphelenchus xyluphilus) Causing the destructive death of pine species. Pine wood nematode is a pine parasitic nematode which is extremely destructive and difficult to control, and after the pine wood nematode is infected by pine trees, the cell wall of the pine bark is destroyed, and the secretion of cellulase and other toxic substances in the pine body interferes with the normal metabolism of the pine trees, so that the tracheids of the xylem of the pine trees generate bubbles, the water transport system of the pine trees is collapsed, and the pine trees are finally killed. The mean death time was from the onset of infection to 30 days of infection. The pine wood nematode disease has strong concealment and dies quickly after outbreak, which is also the difficult point of prevention and treatment. The pine wood nematode is mainly carried by vector insects to spread and spread in nature, and is also an important factor for quick and mass spread of the pine wood nematode, so that the control difficulty of the pine wood nematode is further improved. As far as 2020, 18 provinces (regions and cities) 666 county-level administrative regions in China have pine wilt nematode diseases, the area of the pine wilt nematode diseases reaches 974 ten thousand mu (No. 4 announcement in 2020 of national forestry and grassland bureau), the generation trend of the pine wilt nematode diseases is greatly increased, and 6000 ten thousand hm in China²Pine forest is facing the threat of the pine wood nematode disease pandemic, and the economic and ecological losses are serious. Therefore, there is a need to find an effective way to control the spread of bursaphelenchus xylophilus to effectively control the occurrence of bursaphelenchus xylophilus disease.

Currently, chemical control of bursaphelenchus xylophilus disease is mainly directed to vector insects and bursaphelenchus xylophilus. With the requirement of environmental protection, researchers screen a group of compounds with control potential on the bursaphelenchus xylophilus disease, such as emamectin benzoate, abamectin, rotenone, fosthiazate, matrine, chlorfluazuron, cyhalodiamide, and pyrithion, under the background that the traditional high-toxicity nematocide (carbofuran, clofos, etc.) is forbidden or limited. Part of the pesticide composition obtains obvious control effect on practical application in forests. Therefore, the discovery and development of novel compounds is the key to solving the problems of environmental pollution and controlling pine wilt disease. Because the pine wood nematodes and the pine wood longicorn larvae are in the trunk, the traditional compound and the conventional pesticide application method cannot enable the nematicidal compound to enter the trunk to act on target organisms, and the spreading of the pine wood nematode disease cannot be remarkably controlled. A new generation of succinate dehydrogenase inhibitor (SDHI) nematicidal compounds: the fluopyram has the characteristics of high-efficiency killing activity to nematodes, low dosage, unique action mechanism and no generation of cross resistance with other types of compounds; especially has systemic activity, can be effectively conducted in plants and acts on pests in the plants. Meanwhile, researches show that the action effect of fluopyram on pine wood nematodes is remarkably higher than that of other nematicides. The application of fluopyram in the pine wood nematode disease can well aim at the pine wood nematodes in the trunk, and solves the problem that the traditional thread-killing compound cannot enter the trunk to act on the pine wood nematodes. Therefore, the development of related formulations of fluopyram has important significance for the control of the pine wilt disease.

At present, the compounds with nematicidal activity are mainly applied to forest disease control work in the forms of a drying injection, a suspending agent, powder and the like. In recent years, under the sustainable development of environmental protection, the dry injection technology becomes a research field which is concerned about, and the technology has the characteristics of high liquid medicine utilization rate, wide insecticidal spectrum, environmental protection, safe use, small influence of weather, low pesticide application efficiency, high cost and the like. The stem injection technology principle is that systemic drugs, plant growth regulators, mineral substances and the like enter a tree body along with stem injection, are transported along with a moisture transport system in the tree body longitudinally, can generate transverse diffusion in the transport process, can be conducted from the root to the apical tip blade, and can be used for transmitting, diffusing, retaining, metabolizing and the like of partial drugs to the root through the phloem or directly enter the phloem through the xylem, so that the drugs play a role in the tree. However, in south China, such as Fujian and Guangdong, the average temperature is high throughout the year and the masson pine resin is secreted vigorously, so that when the injection is used, the injection hole of the injection device is blocked by the pine resin, and the liquid cannot be injected into the tree. In addition, in the application of the applied dryer patent (application number 202011106655.9), solvents such as dimethyl sulfoxide and diethylene glycol monobutyl ether and the like have strong water absorption and lipophilicity, and the pipeline for conveying water and oil is easily damaged by a drilling and drying method, so that the phenomenon of bottle expansion caused by backflow of a drying bottle due to the pressure effect in a tree body when the oil and the water are mixed together is caused, and the prevention and treatment effect is influenced.

Disclosure of Invention

In view of the above problems, the present invention aims to provide a suck-back prevention injection drying agent for preventing and treating pine wood nematode diseases.

In order to achieve the purpose, the invention adopts the following technical scheme:

a suck-back prevention dry injection for preventing and treating pine wilt disease comprises the following raw material components in percentage by mass: 1% -12% of fluopyram, 78% -99% of ethyl acetate and water are mixed and then the solvent for preventing suck-back is obtained; mixing ethyl acetate with water to prevent ethyl acetate in the suck-back solvent: the water mass ratio is 4: 1-1: 0.

The suck-back-preventing dry injection for preventing and treating the pine wilt disease comprises the following raw material components in percentage by mass: 1% -12% of fluopyram, 78% -99% of ethyl acetate and water are mixed to form an anti-suck-back solvent, 0% -11% of an emulsifier, 0% -11% of a stabilizer and 0% -11% of a penetrating agent.

The active ingredient of the composition is fluopyram which has trapping and killing effects on the pine wood nematodes, has systemic conduction effect, can be distributed in the interior of a tree body in a short time, can efficiently kill the pine wood nematodes invading the pine, and is used for preventing and treating the pine wood nematode diseases.

The proportion of ethyl acetate and water is adjusted according to the moisture content of rosin secreted by pine trees, so that the solvent can be well balanced with moisture and rosin in the guide pipe and the resin channel, the solvent can be well conducted by the resin channel and the guide pipe of the tree body, and the efficient conduction of effective components in the tree body is promoted.

The emulsifier can make two-phase liquid which is not dissolved originally, wherein one liquid is stably dispersed in the other phase liquid by tiny liquid beads to form opaque or semitransparent emulsion, including but not limited to one or more of diethylene glycol monobutyl ether, sodium dodecyl benzene sulfonate and alkylphenol polyoxyethylene.

The stabilizer is used for preventing and controlling the decomposition or physical deterioration of active ingredients of the pesticide in the storage process, and comprises one or more of phosphorous acid, alkyl phosphite ester, epoxidized soybean oil and epichlorohydrin.

The penetrating agent can promote the active ingredients of the pesticide to enter the inside of the treated object, and comprises but is not limited to one or more of azone and fatty alcohol-polyoxyethylene ether.

Preferably, the suck-back-preventing dry injection for preventing and treating the pine wilt disease comprises the following components in percentage by mass: 8% of fluopyram, 77.4% of ethyl acetate, 8.6% of water, 2% of diethylene glycol monobutyl ether, 2% of epichlorohydrin and 2% of fatty alcohol-polyoxyethylene ether.

The suck-back-preventing stem injection agent for preventing and treating the pine wilt disease is used for injecting stem into host plants with the tree diameter of more than 5 cm. The host plant is a Pinus host plant, including but not limited to Pinus massoniana (A), (B), (C) and (C)Pinus massoniana) "Black pine" (a)P. thunbergii) Xue Song (Chinese white pine)Cedrus deodara) Red pine (Pinus densiflora)P. densiflora) Larch, larch (Larix gmelini) Yunnan pine (1)P. yunnanensis) One kind of (1).

The trunk injection agent adopts a trunk injection method, the total amount of 10 mL of the trunk injection agent in each bottle is used for trunk injection, the aperture of an injection hole is 1-5 mm during trunk injection, and the damage to a tree body is small.

The preparation method of the injection drying agent comprises the following steps: weighing the components in parts by mass, and fully stirring and mixing.

The invention has the following advantages and obvious technical effects:

the main component fluopyram in the drying agent has a systemic conduction function and can be spread throughout the tree body in a short time; the ethyl acetate is a solvent, so that various effective components can be mutually dissolved well, and the situation that the effective components are blocked by turpentine oxidized and agglomerated secreted by a hole-punched wound of a pine tree; the proportion of ethyl acetate and water is adjusted according to the moisture content of rosin secreted by pine trees, so that the solvent can be well balanced with the moisture and the rosin in the guide pipe and the resin channel, the solvent can be well conducted by the guide pipe and the resin channel of the trees, and the prevention and treatment effect is improved.

Drawings

FIG. 1 shows the liquid level of a masson pine bottle which is acted on different drying agents (containing auxiliary agents) with the diameters of 5-15 cm, 16-25 cm and 26-35 cm respectively to descend.

FIG. 2 shows the liquid level of the masson pine which acts on different drying agents (without additives) with the diameters of 5-15 cm, 16-25 cm and 26-35 cm respectively and is injected into a drying bottle to descend.

Fig. 3 is a graph showing the effect of the drying agent.

DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION

The present invention will be described in detail with reference to examples. It should be understood that the scope of the above subject matter is not limited by the following examples, and that all techniques implemented based on the above disclosure are within the scope and protection of the present invention.

Example 1

By taking the formula of the drying agent disclosed in the application No. 202011106655.9 patent as a comparison, on the basis of the formula, the formula of the drying agent with the anti-suck-back effect is screened out through the proportion optimization of ethyl acetate and water, and organic solvents with over-strong water absorbability, such as dimethyl sulfoxide and the like, are deleted, so that the problem that the drying effect is reduced due to rosin secretion in spring, summer and autumn is solved.

TABLE 1 different formulations of the dry injection

The components are shaken and mixed at the room temperature higher than 18 ℃ to obtain the product.

In the selected test field of the pine wood nematode disease area of the masson pine, 180 pieces of the masson pine with the tree diameter of 5-15 cm, 180 pieces of the masson pine with the tree diameter of 16-25 cm and 180 pieces of the masson pine with the tree diameter of 26-35 cm are randomly selected, and then the pesticide application tests of different drying agents are carried out, wherein the pesticide application tests are repeated for 30 pieces each. The trunk injection agents 6-10 are respectively control groups of the trunk injection agents 1-5 except the auxiliary agent, wherein the trunk injection agents 6 and 1 are selected from the same trees for medicine application, the trunk injection agents 7 and 2 are selected from the same trees for medicine application, the trunk injection agents 8 and 3 are selected from the same trees for medicine application, the trunk injection agents 9 and 4 are selected from the same trees for medicine application, the trunk injection agents 10 and 5 are selected from the same trees for medicine application, and the change situation of the liquid level of the trunk injection agents in a trunk injection bottle is observed after three days of implementation.

Example 2

Dry-out applications were performed on 1/2021 and observed on 1/4/2021. As shown in FIG. 1, it is understood from the test results that the larger the diameter of the tree, the higher the resin pressure, and the more the liquid level in the bottle for pouring the small-diameter pinus massoniana was lowered than that of the large-diameter pinus massoniana. The liquid level descending conditions of the drying agent with different water contents are different for the masson pine with three different tree diameters. Firstly, the best trunk injection effect of the trunk injection agent 2 and the trunk injection agent 3 is observed for the masson pine with three tree diameters, and the trunk injection effect is superior to that of the trunk injection agent in the patent application No. 202011106655.9; under the condition of low tree diameter, the effect of the stem injection agent 1 is better, and the stem injection effect of the stem injection agent 4 aiming at different tree diameters is slightly better than that of the stem injection agent 5 on the whole. When no auxiliary agent is used, the implementation effects of the five dry injection agents are similar to those of the corresponding auxiliary agent, and the dry injection agent 2 and the dry injection agent 3 are superior to other dry injection agents on the whole (figure 2).

The drying agent is optimized on the basis of the formula of the drying agent disclosed in the patent application No. 202011106655.9 by combining with the examples 1 and 2, is simpler than the formula of the applied patent, can ensure the prevention and treatment effect on the pine wood nematode disease, further effectively solves the problem that the bottle is expanded due to backflow of the drying bottle caused by the water absorbability and lipophilicity of solvents such as dimethyl sulfoxide, diethylene glycol monobutyl ether and the like in the conventional drying agent, adjusts the proportion of ethyl acetate and water according to the moisture content of pine resin secreted by pine trees, enables the tree body conduit and the resin channel to conduct the solvents well, and greatly promotes the prevention and treatment effect of the drying agent on the pine wood nematode disease.

The present invention is described in detail with reference to the above embodiments. It should be noted that the above embodiments are only for illustrating the present invention. Numerous alternatives and modifications can be devised by those skilled in the art without departing from the spirit and scope of the invention, which should be construed as within the scope of the invention.