阅读说明：本技术 一种具有抗霾清肺功效的罗汉果蜜及其生产方法 (It is a kind of have effects that anti-haze clearing lung-heat Siraitia grosvenorii honey and its production method ) 是由 李伟 黄华学 赵冠宇 刘永胜 叶桂芳 陈江林 黄�俊 贺进军 于 2019-08-13 设计创作，主要内容包括：一种具有抗霾清肺功效的罗汉果蜜及其生产方法,该罗汉果蜜包括罗汉果苷V、罗汉果总苷、葡萄糖、果糖、蔗糖、甘露醇和水。其生产法方法为：(1)将罗汉果鲜果榨汁,收集果汁和果渣,冷水渗漉提取果渣,得渗漉液,合并果汁和渗漉液,得罗汉果提取液；(3)向罗汉果提取液中加入氢氧化钙,调节pH值至碱性,产生沉淀,静置,离心,收集离心滤液,得絮凝滤液；(4)向絮凝滤液中通入二氧化碳或空气,调节pH值至中性,产生沉淀,静置,离心,收集离心滤液,得中和滤液；(5)纳滤中和滤液,收集截留液,得脱盐液；(6)浓缩：将脱盐液用蒸发器减压浓缩,即成。本发明罗汉果蜜,色泽浅,口感与蔗糖极其相似；本发明生产方法工艺简单,成本低廉,适合工业化批量生产。(A kind of Siraitia grosvenorii with anti-haze clearing lung-heat is sweet and its production method, the Siraitia grosvenorii honey include momordica grosvenori glycoside V, the total glycosides of Siraitia grosvenorii, glucose, fructose, sucrose, sweet dew alcohol and water.Its working system method are as follows: (1) Siraitia grosvenorii fresh fruit is squeezed the juice, collect fruit juice and pomace, cold water seepage pressure effects pomace, obtain percolate, merged fruit juice and percolate, obtain Siraitia grosvenorii extracting solution；(3) calcium hydroxide is added into Siraitia grosvenorii extracting solution, adjusts pH value to alkalinity, generates precipitating, stand, centrifugal filtrate is collected in centrifugation, and must flocculate filtrate；(4) it is passed through carbon dioxide or air into flocculation filtrate, adjusts pH value to neutrality, generates precipitating, stand, centrifugation collects centrifugal filtrate, must neutralize filtrate；(5) in nanofiltration and filtrate, trapped fluid is collected, desalinization liquor is obtained；(6) it is concentrated: desalinization liquor is concentrated under reduced pressure with evaporator,.Siraitia grosvenorii honey of the present invention, lighter color, mouthfeel are extremely similar to sucrose；Production method simple process and low cost of the present invention is suitble to industrialized mass production.)

1. a kind of Siraitia grosvenorii honey with anti-haze clearing lung-heat, which is characterized in that including momordica grosvenori glycoside V, the total glycosides of Siraitia grosvenorii, grape Sugar, fructose, sucrose, sweet dew alcohol and water.

2. a kind of Siraitia grosvenorii honey with anti-haze clearing lung-heat, which is characterized in that by weight, including 0.1 ~ 15.0 portion of Siraitia grosvenorii Glycosides V, the total glycosides of 0.15 ~ 30.0 portion of Siraitia grosvenorii, 5.0 ~ 30.0 parts of glucose, 5.0 ~ 30.0 parts of fructose, 0.1 ~ 30 portion of sucrose, 0.1 ~ 15.0 portions of mannitol and 20.0 ~ 60.0 parts of water.

3. having effects that the Siraitia grosvenorii honey of anti-haze clearing lung-heat according to claim 2, which is characterized in that the Siraitia grosvenorii honey extracts From Siraitia grosvenorii fresh fruit, by weight comprising: 3.0 ~ 5.0 parts of momordica grosvenori glycoside Vs, the total glycosides of 5.0 ~ 7.0 portions of Siraitia grosvenoriis, 20.0 ~ 25.0 Part glucose, 10.0 ~ 15.0 parts of fructose, 10 ~ 15 portions of sucrose, 2 ~ 3 portions of mannitol and 25.0 ~ 53.0 parts of water.

4. a kind of production method of the Siraitia grosvenorii honey with anti-haze clearing lung-heat, which comprises the following steps:

(1) juicing and diacolation: Siraitia grosvenorii fresh fruit is squeezed the juice, and only broken shell, not broken fruit seed, collect fruit juice and pomace respectively；Then Seepage pressure effects are carried out to pomace using cold water, obtain percolate；Merge fruit juice and percolate, obtain Siraitia grosvenorii extracting solution, it is preferred to use Extrusion juice extractor juicing；

(2) flocculation and enzyme deactivation: calcium hydroxide being added into Siraitia grosvenorii extracting solution, adjusts pH value to alkalinity, and generation precipitating is stood, Centrifugal filtrate is collected in centrifugation, and must flocculate filtrate；

(3) it neutralizes and precipitates: being passed through carbon dioxide or air into flocculation filtrate, adjusting pH value to neutrality generates precipitating, quiet It sets, is centrifuged, collect centrifugal filtrate, filtrate must be neutralized；

(4) desalination: filtrate nanofiltration membrane will be neutralized, collects trapped fluid, obtains desalinization liquor；

(5) it is concentrated: desalinization liquor is concentrated under reduced pressure with evaporator, obtain Siraitia grosvenorii honey；The evaporator five imitates downward film evaporator.

5. having effects that the production method of the Siraitia grosvenorii honey of anti-haze clearing lung-heat according to claim 4, which is characterized in that step (1) in, the temperature of the cold water is 0~30 DEG C；The cold water usage is 0.5~1.2 times of Siraitia grosvenorii fresh fruit weight, the infiltration The flow velocity filtered is 0.2~1.2 BV/ hours；It is furthermore preferred that the dosage of the cold water is 0.8~1 times of Siraitia grosvenorii fresh fruit weight, The flow velocity of the percolate is 0.6~1.0BV/h.

6. the production method of the Siraitia grosvenorii honey with anti-haze clearing lung-heat according to claim 4 or 5, which is characterized in that step Suddenly in (2), the dosage of the calcium hydroxide is the 0.01%~10% of Siraitia grosvenorii fresh fruit weight, and adjusting pH value is 8.6~13.0；It is excellent Choosing, in step (2), the dosage of the calcium hydroxide is the 2~4% of Siraitia grosvenorii fresh fruit weight, adjust pH value be 11.5~ 12.5。

7. having effects that the production method of the Siraitia grosvenorii honey of anti-haze clearing lung-heat according to any one of claim 4~6, feature exists In in step (3), the carbon dioxide or the pH value of air conditioning of being passed through is 6.5~8.5；More preferably, described to be passed through two The pH value of carbonoxide or air conditioning is 7.0~7.5.

8. having effects that the production method of the Siraitia grosvenorii honey of anti-haze clearing lung-heat according to any one of claim 4~7, feature exists In in step (4), the molecular cut off of the nanofiltration membrane is 80~250Da, and the pressure of filtering is 0.5~1.2MPa, and nanofiltration is extremely Conductivity in trapped fluid is 0.1~20us/cm；Preferably, in step (4), the molecular cut off of the nanofiltration membrane is 100~ 200Da, the pressure of filtering are 0.6~1MPa, and conductivity of the nanofiltration into trapped fluid is 10~20us/cm.

9. having effects that the production method of the Siraitia grosvenorii honey of anti-haze clearing lung-heat according to any one of claim 4~8, feature exists In, in step (5), the first effect and end effect evaporating temperature of the five effects downward film evaporator are followed successively by 60~65 DEG C, 40~45 DEG C, The mass percentage for being concentrated into solid content is 40.0%~80.0%.

10. having effects that any one of the Siraitia grosvenorii honey of anti-haze clearing lung-heat or claim 3-9 institute described in any one of claims 1 to 3 The Siraitia grosvenorii honey of production method preparation is stated in preparing food, drug, flavouring, health care product, cosmetics and/or flavors and fragrances Using.

Technical field

The present invention relates to a kind of Fructus Monordicae extract and its production methods, and in particular to a kind of to have effects that anti-haze clearing lung-heat Siraitia grosvenorii honey and its production method.

Background technique

Siraitia grosvenorii mainly originates in the Lingui County and Yongfu county of Guilin City, is described as angle fruit by people.The fruit of Siraitia grosvenorii contains There is mogroside, be a kind of triterpene glucoside with sweet taste, sugariness highest is not generated than 300 times of sucrose sweet tea Heat is the rare raw material of a kind of beverage, confectionery industry, is the best substitute of sucrose.

It is safe and non-toxic that mogroside, which belongs to low in calories, Gao Tiandu, non-nutritive, non-fermented type sweetener as food, , the regulation in state compulsion standard " GB2760 food additives use standard ", sweet Momordica grosvenori quantity-unlimiting can be used for all kinds of foods Product.

Often drink " Luohan " fruit tea can be drunk to prevent a variety of diseases suitable for many coughs of phlegm and pertussis patient.Siraitia grosvenorii is not It is only a kind of relieving cough and reducing sputum Chinese medicine, even more a kind of tea-drinking of taste sweetness, specific effect includes: that clearing heat and moistening lung, relieving sore-throat are opened Sound, relax bowel and defecation improve immune function, protection liver, antibacterial, hypoglycemic, antitumor etc..

Research of the country to Fructus Monordicae extract especially grosvenor momordica fruit juice, with the innovation of extracting and developing technology, It is increasingly mature.

CN104661535A discloses a kind of preparation method of sweet juice composition, is using Siraitia grosvenorii fresh fruit as raw material, through 60 DEG C or more high-temperature water extract, clarification, cation exchange resin and anion exchange resin and mixed bed decoloration, obtain Siraitia grosvenorii sweet juice.This method has largely used cation exchange resin and anion exchange resin and mixed bed to decolourize, practical A large amount of sewage will be generated in resin regeneration step in production, environmental pollution is serious.

CN107969532A discloses a kind of Siraitia grosvenorii flavor instant drink and preparation method thereof, is to be with Siraitia grosvenorii fresh fruit Raw material obtains the instant drink of Siraitia grosvenorii flavor through broken, 60~100 DEG C of hot water extractions, clarification, ultrafiltration, chromatography, concentration Product.This method has largely used steam, and during high temperature extracts and high temperature is concentrated, " beauty will occur for protein and carbohydrate Maillard reaction " generates dark polymer, and residual is difficult to remove in the product, has not only deepened product color, but also can be to product mouth Sense has detrimental effect.

CN108094772A discloses a kind of method for preparing grosvenor momordica fruit juice, is using Siraitia grosvenorii fresh fruit as raw material, through superelevation Enzymatic hydrolysis, super-pressure enzyme deactivation, filters pressing or centrifugation, yeast processing, activated carbon chromatography column processing, ultrafiltration, nanofiltration are pressed, sieve is obtained Chinese fruit fruit juice.This method complex steps, the high requirements on the equipment, high production cost, are not suitable for industrialized mass production.

CN101283831 discloses a kind of decoloration method for preparing grosvenor momordica fruit juice and the decoloration arhat by the method preparation Fruit fruit juice through washing, inactivation, is crushed, enzyme extraction, micro-filtration, concentration, resin decolorization, concentration using Siraitia grosvenorii fresh fruit as raw material And etc., obtain decoloration grosvenor momordica fruit juice.This method also needs a large amount of decolorizing resin, with high costs, is not suitable for industrializing Batch production.

CN1663469A discloses a kind of method for preparing grosvenor momordica fruit juice, is to mention using Siraitia grosvenorii fresh fruit as raw material through boiling water It takes, centrifugal filtration, ebuillition of heated make albuminous degeneration precipitating, removing bitter substance, plate compression, micro-pore-film filtration, nanofiltration concentration And etc., obtain grosvenor momordica fruit juice.This method will inevitably occur " Maillard reaction ", generate in extracting in boiling water step Dark polymer, and it is subsequent without decoloration the step of, be unable to get the pure grosvenor momordica fruit juice of lighter color, flavour.

The emphasis of above-mentioned technology is all that the main sweet ingredient --- mogroside --- in Siraitia grosvenorii carries out Research.However, there are one important active constituents in Siraitia grosvenorii, it is ignored always, that is, mannitol.

Mannitol is good diuretics in medicine, can reduce intracranial pressure, intraocular pressure and treatment renal drug, dehydrant, food Sugared substitute also serves as the excipient of tablet and the diluent of solid, liquid.Formula mannitol injection liquid is as hypertonic depressor Clinical rescue especially brain disorder rescues a kind of common medicine, has the fast curative effect of decompression required by reducing intracranial pressure drug quasi- True feature.Mannitol can improve plasma osmolarity after entering in vivo, make tissue dewatering, can reduce intracranial pressure and intraocular pressure, from It after glomerular filtration, is not easy to increase osmotic pressure of urine by ball reabsorption on kidney, taken out of large quantity of moisture and be dehydrated, for outside cranium brain Hurt, oedema caused by cerebral hypoxia, caused oedema after large-area burns, ascites glaucoma caused by renal failure.And Early stage acute renal insufficiency can be prevented and treated.

According to the literature, the sweet dew alcohol component that Siraitia grosvenorii contains plays the role of cough-relieving, clearing lung-heat.Therefore, Siraitia grosvenorii is cut thin Piece is brewed with boiled water, is directly drunk as tea-drinking, and chronic pharyngitis and sphagitis can be effectively prevented.

With the development of society, the economy of high density population and social activities will necessarily discharge a large amount of fine particle (PM 2.5), once discharge is more than atmospheric air circulation ability and carrying degree, fine particle concentration is by continued accumulation, at this time if by quiet steady Weather etc. influences, and large-scale haze easily occurs.The deeper harm of haze is fertility and the baby that can also influence the mankind The health of child.Expert claims, and is exposed to the animal of rich air pollutant in embryo and infant period, compare adult The fertility for the group that period is exposed in pollution environment is decreased significantly.

Siraitia grosvenorii is a kind of distinctive plant in China, is the rare Chinese medicine of dual-purpose of drug and food that the Ministry of Public Health announces in the first batch, Itself have effects that clearing heat and moistening lung, raising immune function etc. are many.Therefore, adaptation to local conditions develops one kind using Siraitia grosvenorii as raw material Have effects that the Siraitia grosvenorii honey of anti-haze clearing lung-heat is extremely urgent, it is of far-reaching significance.

Summary of the invention

The technical problem to be solved by the present invention is to overcome drawbacks described above of the existing technology, provide a kind of with anti- The Siraitia grosvenorii honey and its production method of haze clearing lung-heat effect, the Siraitia grosvenorii honey have effects that anti-haze clearing lung-heat, and sweet pure in smell, flavour Sweet, mouthfeel is saturated, without bitter taste and metallic taste, and mouthfeel is extremely similar to sucrose；Without decolorization process in the production method, it is not necessarily to Using decolorizing resin, the dosage of steam is few, and simple process, and strong operability is low in cost, is suitble to industrialized mass production.

The technical solution adopted by the present invention to solve the technical problems is as follows:

It is a kind of have effects that anti-haze clearing lung-heat Siraitia grosvenorii honey, including the total glycosides of momordica grosvenori glycoside V, Siraitia grosvenorii, glucose, fructose, sucrose, Sweet dew alcohol and water.

Preferably, including 0.1 ~ 15.0 part of momordica grosvenori glycoside V, the total glycosides of 0.15 ~ 30.0 portion of Siraitia grosvenorii, 5.0 ~ 30.0 portions of grapes Sugar, 5.0 ~ 30.0 parts of fructose, 0.1 ~ 30 portion of sucrose, 0.1 ~ 15.0 portion of mannitol and 20 ~ 60 parts of water.

Preferably, the Siraitia grosvenorii honey extracts from Siraitia grosvenorii fresh fruit, by weight comprising: 3.0 ~ 5.0 parts of Momordia grosvenori aglycones V, the total glycosides of 5.0 ~ 7.0 portions of Siraitia grosvenoriis, 20.0 ~ 25.0 parts of glucose, 10.0 ~ 15.0 parts of fructose, 10 ~ 15 portions of sucrose, 2 ~ 3 parts of sweet dews Pure and mild 25 ~ 53 parts of water.

" the total glycosides of Siraitia grosvenorii " includes momordica grosvenori glycoside V, and further includes Siraitia grosvenorii 11- oxygroup glycosides V, Momordia grosvenori aglycone III, sieve One of Momordia grosvenori aglycones such as Chinese fruit glycosides IV, Simon glycosides are a variety of.

The present invention has effects that the component raw material of the Siraitia grosvenorii honey of anti-haze clearing lung-heat, can be obtained by routine business approach. Therefore, it prepares, each raw material component can be weighed, mixture forms by the component, proportion.But it is preferred that following productions of the invention Method method.

The present invention has effects that the production method of the Siraitia grosvenorii honey of anti-haze clearing lung-heat, comprising the following steps:

(1) juicing and diacolation: Siraitia grosvenorii fresh fruit is squeezed the juice, and only broken shell, not broken fruit seed, collect fruit juice and pomace respectively；Then Seepage pressure effects are carried out to pomace using cold water, obtain percolate；Merge fresh fruit fruit juice and percolate, obtains Siraitia grosvenorii extracting solution；It is preferred that It is squeezed the juice using extrusion juice extractor；

(2) flocculation and enzyme deactivation: calcium hydroxide being added into Siraitia grosvenorii extracting solution, adjusts pH value to alkalinity, and generation precipitating is stood, Centrifugal filtrate is collected in centrifugation, and must flocculate filtrate；

(3) it neutralizes and precipitates: being passed through carbon dioxide or air into flocculation filtrate, adjusting pH value to neutrality generates precipitating, quiet It sets, is centrifuged, collect centrifugal filtrate, filtrate must be neutralized；

(4) desalination: filtrate nanofiltration membrane will be neutralized, collects trapped fluid, obtains desalinization liquor；

(5) it is concentrated: desalinization liquor is concentrated under reduced pressure with evaporator, obtain the Siraitia grosvenorii honey；The evaporator preferably uses five effects to drop Kestner long-tube evaporator.

It is preferred that being squeezed the juice using extrusion juice extractor, juice-extracting process is to collect arhat juice and water therein without heating Soluble components method the most energy-efficient；The process of juicing only breaks shell, does not break fruit seed, it can be ensured that squeezes out in the fruit juice overwhelming majority While, it also can avoid the ingredients such as the bad flavor substance (bitter, astringent taste) discharged after arhat fruit seed is broken and pigment and be mixed into Fruit juice influences the taste and flavor of final products；It is that will squeeze the juice by the purpose of Siraitia grosvenorii shell and fruit seed cold water seepage pressure effects Pomace afterwards --- Siraitia grosvenorii shell and fruit seed --- sweet tea glycosides remained on surface and other water soluble ingredients leach, to ensure sweet tea glycosides And the yield of other water soluble ingredients.

Preferably, in step (1), the temperature of the cold water is 0~30 DEG C.

Preferably, in step (2), the dosage of the cold water is 0.5~1.2 times of Siraitia grosvenorii fresh fruit weight, the stream of diacolation Speed is 0.2~1.2 BV/ hours.During diacolation, if the dosage of pure water is very few or the flow velocity of diacolation is too fast, arhat all will lead to Fruit shell and fruit seed sweet tea glycosides remained on surface and other water soluble ingredients can not be leached thoroughly, cause yield relatively low；If pure water Dosage is excessively or the flow velocity of diacolation is excessively slow, will all cause the waste of material and the energy.More preferably, described cold in step (1) The dosage of water is 0.8~1 times of Siraitia grosvenorii fresh fruit weight, and the flow velocity of the percolate is 0.6~1.0BV/h.

Preferably, in step (2), the dosage of the calcium hydroxide is the 0.01%~10% of Siraitia grosvenorii fresh fruit weight, is adjusted PH value is 8.6~13.0.Calcium hydroxide is added, the first purpose is that the pectin in Siraitia grosvenorii extracting solution is flocculated and precipitated；Purpose Second is that the ingredients such as polypeptide, protein and enzyme in Siraitia grosvenorii extracting solution are denaturalized and are precipitated, remove Siraitia grosvenorii fresh fruit itself Jerky taste.If the dosage of calcium hydroxide it is very few or adjust pH value it is too low, be all unable to reach above-mentioned purpose；If calcium hydroxide Dosage is excessively or the pH value of adjusting is excessively high, will all cause the waste of material and the energy.

More preferably, in step (2), the dosage of the calcium hydroxide is the 2~4% of Siraitia grosvenorii fresh fruit weight, adjusts pH Value is 11.5~12.5.

Preferably, in step (3), the carbon dioxide or the pH value of air conditioning of being passed through is 6.5~8.5.Use dioxy Change carbon or air conditioning pH value to neutrality, the first purpose is the alkali neutralized in flocculation liquid liquid；The second purpose is to make liquid middle reaches of flocculating From calcium ion be converted into precipitation of calcium carbonate, to reduce the treating capacity of subsequent desalting steps.If the pH value adjusted is excessively high, by nothing Method reaches above-mentioned purpose；If the pH value adjusted is too low, the waste of material and the energy not only will cause, the precipitation of calcium carbonate of generation is also The calcium bicarbonate for being dissolved in water will be generated with excessive carbon dioxide reaction, be equally unable to reach above-mentioned purpose.

Preferably, in step (4), the molecular cut off of the nanofiltration membrane is 80~250Da, the pressure of filtering is 0.5~ 1.2MPa, conductivity of the nanofiltration into trapped fluid are 0.1~20us/cm(25 DEG C).It is to remove using the purpose of nanofiltration membrane Neutralize the salinity in filtrate.The conductivity of if molecular cut off of nanofiltration membrane is too small, filters hypotony or nanofiltration retentate fluid It is excessively high, all salinity cannot thoroughly be removed；If the molecular cut off of nanofiltration membrane it is excessive or filtering hypertonia, be likely to Cause the water soluble ingredient (organic matters such as carbohydrate) in Siraitia grosvenorii through nanofiltration membrane, causes the yield of Siraitia grosvenorii honey relatively low.

More preferably, in step (4), the molecular cut off of the nanofiltration membrane is 100~200Da, and the pressure of filtering is 0.6~1MPa.Preferably, in step (5), the first effect and end effect evaporating temperature of the five effects downward film evaporator be followed successively by 60~ 65 DEG C, 40~45 DEG C.Using five effect downward film evaporators purpose be, save steam energy consumption, at low temperature, the short time complete it is dense The process of contracting, prevents in concentration process, and the flavor components in Siraitia grosvenorii honey are because occurring gelatinization, coking at high temperature for a long time or dividing Solution retains the excellent flavor of Siraitia grosvenorii honey to the greatest extent.

The Siraitia grosvenorii honey for having effects that anti-haze clearing lung-heat or the Siraitia grosvenorii honey of production method preparation prepare food, Application in drug, flavouring, health care product, cosmetics and/or flavors and fragrances.The mechanism of the method for the present invention: using only broken shell, The extracting mode that " the squash type juicing " and " cold water diacolation " of not broken fruit seed combines so that water solubility in Siraitia grosvenorii fresh fruit at Sub-dip extracting rate is high, but the leaching of pigment is few；In addition, entire technical process is extracted without high temperature, thermophilic digestion or high temperature are concentrated The step of, with carbohydrate " Maillard reaction " can not occur for protein, polypeptide in Siraitia grosvenorii extract etc., generate dark poly- Object is closed, therefore, the method for the present invention does not need to decolourize, so that it may obtain the Siraitia grosvenorii honey of lighter color, clear.

Beneficial effects of the present invention are as follows:

(1) Siraitia grosvenorii honey of the present invention not only includes the sweet tastes objects such as momordica grosvenori glycoside V, the total glycosides of Siraitia grosvenorii, sucrose, fructose and glucose Matter further includes the mannitol with effect of clearing away lung-heat itself, the study found that the sweet dew of Siraitia grosvenorii honey of the invention compared to same dosage Alcohol has more excellent effect of clearing away lung-heat, and also has excellent anti-haze effect；

(2) the close Siraitia grosvenorii produced using the method for the present invention is in colourless, faint yellow or brown color clear dense fluids, nothing It needs to decolourize, avoids the use of decolorizing resin, reduce production cost, and avoid decolorizing resin regenerative process and generate largely Sewage；

(3) in the preferred embodiment of production method of the present invention, the combination of " squash type juicing " and " cold water diacolation " two kinds of extracting modes, And five effect downward film evaporator utilization significantly saved energy consumption so that the dosage of steam is few in production process；

(4) the method for the present invention production Siraitia grosvenorii honey, containing a variety of nutriments such as momordica grosvenori glycoside V, glucose, fructose, sucrose with And mannitol etc., have effects that anti-haze clearing lung-heat, it is fragrant to can be applied to food, drug, flavouring, health care product, cosmetics and essence In material, and its lighter color, pure in smell, flavour is sweet, mouthfeel saturation, without bitter taste and metallic taste, mouthfeel and sucrose extremely phase Seemingly, also direct-edible；

(5) production method simple process of the present invention, strong operability is low in cost, safety and environmental protection, is suitble to industrial mass raw It produces.

Specific embodiment

Below with reference to embodiment, the invention will be further described.

Siraitia grosvenorii fresh fruit used in the embodiment of the present invention is picked before juicing and cleaning step, the standard picked are as follows: choosing Select the complete and complete Siraitia grosvenorii fresh fruit of saccharification.

Siraitia grosvenorii fresh fruit used in the embodiment of the present invention is purchased from the organic arhat of Hunan Hua Cheng living resources limited liability company Fruit planting base, wherein the content of momordica grosvenori glycoside V is 0.47%；In embodiment 4 and comparative example 1,2, the momordica grosvenori glycoside V that uses and The total glycosides of Siraitia grosvenorii is bought in Hu'nan Huacheng Biological Resources Co., Ltd..

Chemical reagent used in the embodiment of the present invention is obtained by routine business approach unless otherwise specified.