阅读说明：本技术 一种无添加的天然罗汉果糖浆及其制备方法和用途 (A kind of no added natural Siraitia grosvenorii syrup and its preparation method and application ) 是由 黄华学 刘庚贵 刘永胜 于 2019-08-29 设计创作，主要内容包括：本发明涉及一种无添加的天然罗汉果糖浆,以鲜罗汉果生产过程的吸附树脂废水为原料制得,具体为：葡萄糖14～24％、果糖16～24％、蔗糖1～14％、甘露醇1～8％、水40～50％,其余成分≤2％,其从鲜罗汉果生产过程中的吸附树脂废液经过精制、脱色、脱酸、去农残和重金属,浓缩等步骤得到,除罗汉果、无籽罗汉果和水外,无其它添加物,是一种纯天然的无添加罗汉果糖浆。按照本发明制备方法,能够有效获得口感好的风味罗汉果饮品,也可以作为食品,饮品,乳品、保健品,药品等中的添加物。本发明制备方法简单易行,所得产品经济价值高,排放废液达到环保标准,不用进一步处理,是一种对罗汉果生产废液的综合利用方法,经济价值高,适合工业化大规模推广。(The present invention relates to a kind of no added natural Siraitia grosvenorii syrup, it is made by raw material of the absorption resin wastewater of fresh fructus momordicae production process, specifically: glucose 14~24%, fructose 16~24%, sucrose 1~14%, mannitol 1~8%, water 40~50%, remaining ingredient≤2%, it passes through purification from the absorption resin waste liquid in fresh fructus momordicae production process, decoloration, depickling, goes agriculture residual and heavy metal, concentration and etc. obtain, in addition to Siraitia grosvenorii, seedless monordica grosvenori and water, it is a kind of pure natural no added Siraitia grosvenorii syrup without other additives.According to preparation method of the present invention, flavor arhat fruit beverage in good taste can be effectively obtained, can also be used as food, drink, dairy products, health care product, the additive in drug etc..Preparation method is simple by the present invention, and products obtained therefrom economic value is high, and discharge waste liquid reaches environmental protection standard, is the method for comprehensive utilization of a kind of pair of Siraitia grosvenorii production waste liquid without being further processed, economic value is high, is suitble to industrialization large-scale promotion.)

1. a kind of no added natural Siraitia grosvenorii syrup, is made by raw material of the absorption resin wastewater of fresh fructus momordicae production process, tool Body are as follows: glucose 14~24%, fructose 16~24%, sucrose 1~14%, mannitol 1~8%, water 40~50%, remaining ingredient ≤ 2%.

2. natural Siraitia grosvenorii syrup as described in claim 1, which is characterized in that its solid content is that the dilution of 1%w/v is water-soluble Absorbance≤0.2 of the liquid at 420nm, preferably absorbance≤0.1, more preferable absorbance≤0.05, pol are 65~70brix.

3. the preparation method of natural Siraitia grosvenorii syrup as claimed in claim 1 or 2, includes the following steps:

(1) purification of three-level UF membrane and concentration: the three-level film is respectively ultrafiltration membrane, nanofiltration membrane and reverse osmosis membrane；

(2) depickling: organic acid is removed with physical adsorption way with macroporous absorbent resin, obtains depickling liquid；

(3) physical decolorization and filtering；

(4) take off that agriculture is residual and heavy metal；

(5) it is concentrated.

4. preparation method as claimed in claim 3, which is characterized in that in step (1), the ultrafiltration retaining molecular weight is 2KD~3KD, the nanofiltration retaining molecular weight are 500~800, and the reverse osmosis membrane is to sodium chloride rejection > 99%；

Preferably, in step (1), the material of the ultrafiltration membrane is polyether sulfone, and the material of nanofiltration membrane and reverse osmosis membrane is polyamide；

It is further preferred that in step (1), 1.0~2.0MPa of operating pressure of the ultrafiltration membrane, nanofiltration membrane and reverse osmosis membrane 2.0~3.0MPa of operating pressure.

5. preparation method as claimed in claim 3, which is characterized in that in step (2), the macroporous absorbent resin is selected from benzene second Ene-type, divinylbenzene types, polyacrylate, acid/acrylic amide type macroporous absorbent resin, physically Adsorption organic acid and Phenolic substances；

Preferably, the granularity of the macroporous absorbent resin is 30~80 mesh；

It is further preferred that the macroporous absorbent resin dosage is every 1000L reverse osmosis membrane concentrate, macroporous absorbent resin is used 120~200kg.

6. preparation method as claimed in claim 3, which is characterized in that in step (3), the physical decolorization uses activated carbon, The Powdered Activated Carbon requirement: iodine sorption value >=900mg/g, Methylene blue adsorption >=9mL；Preferably, in step (3), institute The dosage for stating active carbon is every 1000L depickling liquid, with 5.0~15.0kg of Powdered Activated Carbon.

7. preparation method as claimed in claim 3, which is characterized in that in step (4), the de- agriculture is residual, used in removing heavy-metal Resin includes gel-type or macroporous type acrylic resin, gel-type or macroporous type phenylethylene resin series；

Preferably, the de- agriculture is residual, the granularity of resin used in removing heavy-metal is 30~80 mesh；

It is further preferred that the de- agriculture is residual and the resin dedicated dosage of removing heavy-metal is every 1000L depickling liquid, it is residual and de- with de- agriculture Resin dedicated heavy metal is 40~80kg.

8. preparation method as claimed in claim 3, which is characterized in that in step (5), the concentration includes under normal temperature condition Reverse osmosis membrane concentration and vacuum concentration.

9. preparation method as claimed in claim 3, which comprises the steps of:

(1) purification of three-level UF membrane and concentration:

(1) the absorption resin wastewater of fresh fructus momordicae production process is pumped into organic membrane system and carries out the 1st purification and removal of impurities, selection Ultrafiltration retaining molecular weight is 2KD~3KD, and collection membrane downstream liquid, that is, refined liquid obtains ultrafiltration membrane refined liquid；

(2) by ultrafiltration membrane refined liquid be pumped into organic membrane system carry out the 2nd time purification and decoloration, select nanofiltration retaining molecular weight for 500~800, collection membrane downstream liquid, that is, refined liquid obtains nanofiltration membrane refined liquid；

(3) nanofiltration membrane refined liquid is pumped into reverse osmosis membrane system, film is concentrated into 5~10Brix, obtains reverse osmosis membrane concentrate；

(2) depickling: reverse osmosis membrane concentrate is pumped into depickling macroporous adsorbent resin special chromatography gravity tank, is led in a manner of flowing automatically Resin column is crossed, into after complete material, with 1~3 times of volume purifying washing column of resin column, collects into column efflux and water lotion, obtains de- Acid solution；

(3) physical decolorization and filtering: Powdered Activated Carbon is added into depickling liquid, is sufficiently stirred and is uniformly mixed under room temperature, keeps 20~30min, plate-frame filtering obtain the colourless destainer of clear；

(4) take off that agriculture is residual and heavy metal: destainer is residual resin dedicated by de- agriculture and/or removing heavy-metal is resin dedicated takes off respectively Except agriculture is residual and heavy metal, column is washed into after complete material, then with the purifying of 2~3 times of resin volumes, by effective component remaining in column It washes out, merges efflux and water lotion, obtain removing heavy-metal liquid；

(5) film concentration and vacuum concentration:

(1) reverse osmosis membrane is concentrated: removing heavy-metal liquid being concentrated into 5~10Brix by reverse osmosis membrane complete set of equipments, obtains film concentration Liquid；

(2) it is concentrated in vacuo: film concentrate is concentrated in vacuo, control Wen Du≤65 DEG C processed, very sky≤0.085MPa, it is concentrated into 60~ 70Brix obtains no added natural Siraitia grosvenorii syrup.

10. any one of the no added natural Siraitia grosvenorii syrup as claimed in claim 1 or 2 or claim 3-9 preparation method The purposes of obtained no added natural Siraitia grosvenorii syrup, which is characterized in that as including food, drink, dairy products, health care product and medicine Component of the product in interior product.

Technical field

The invention belongs to plant extract separation technology fields, and in particular to a kind of no added natural Siraitia grosvenorii syrup and its Preparation method and purposes.

Background technique

Siraitia grosvenorii (Siraitia grosvenorii (Swingle) C.Jeffrey), English name: fructus Momordicae, the fruit of the perennial liana of Curcurbitaceae.Alias sweating fruit, balloonvine heartseed herb, Semen Momordicae, invaluable, arhat Table, naked tortoise bar are described as " angle fruit, leaf heart, dioecism, summer-flowering, seed in autumn by people.Mainly originate in Guangxi Guilin City, Zhuang autonomous region, Longjiang township, Yongfu county, the town Bai Shou, Multinational Autonomous County of Longsheng and Hunan Province's Huaihua, Yongzhou, Shaoyang etc. Ground.Siraitia grosvenorii has green peel fruit, long beach fruit, wax gourd fruit, draws the kinds such as river fruit, red comospore, tung nut, and the plantation of green peel fruit is more.Sieve Chinese fruit is also one of the dual-purpose of drug and food material that country ratifies in the first batch, and primary efficacy is can moistening lung to arrest cough, resolving sputum of promoting the production of body fluid.

Seedless monordica grosvenori is to plant the triploid of Siraitia grosvenorii and diploid staminiferous plant, diploid is used when blooming Female plant artificial pollination of the staminiferous plant to triploid, obtain seedless monordica grosvenori after female plant fruiting.

Siraitia grosvenorii and seedless monordica grosvenori flavor rich in and nutritional ingredient and triterpene saponin non-saccharide sweet taste at Point.

One, chemical component

Siraitia grosvenorii and seedless monordica grosvenori contain Siraitia grosvenorii triterpenoid saponin, and there are also sucrose, glucose (glucose), fructose (fructose) account for 14%, more than ten kinds of essential amino acids, protein, fatty acid, flavone compound, vitamin C and 26 kinds of inorganic elements such as manganese, iron, nickel, selenium, tin, iodine, molybdenum and microelement etc..

1, cucurbitane-glycoside class, this kind of compound are principle active components in Siraitia grosvenorii, they have common aglycon knot Structure.This substance is sweet substance or slightly sweet substance in addition to minority in Siraitia grosvenorii.Wherein momordica grosvenori glycoside V is main sweet taste Ingredient, its nontoxic, low in calories, Gao Tiandu, thermal stability are good, are one of the substances as natural sweetener exploitation.

2, protein, amino acid, 7.1%-7.8% containing protein in Siraitia grosvenorii dry fruit.In its hydrolysate, tryptophan is removed Not measured outer, 18 kinds of amino acid are complete.In the amino acid of measurement, content is higher glutamic acid (108.2-113.3mg/ Kg), aspartic acid (93.9-112.5mg/kg), valine (52.5-55.5mg/kg), alanine (49.9-66.8mg/kg), Leucine (48.5-56.7mg/kg).Glutamic acid is the maximum nonessential amino acid of requirement in human body, is synthesizing glutathion Important source material, it is seen then that Siraitia grosvenorii nutritive value with higher.

3, inorganic elements contains 24 kinds of inorganic elements in Lo Han Guo fruit, wherein the essential trace elements of the human body and extensively Member is known as 16 kinds, and the higher member of content is known as potassium (12290mg/kg), calcium (667mg/kg), magnesium (550mg/kg).In Siraitia grosvenorii The selenium contained reaches 0.186mg/kg, is 2 times~4 times of grain, and elemental selenium is in coronary heart disease prevention and treatment, anti-aging, anticancer aspect It has a better effect.

Two, main chemical compositions structural formula

The product of the invention patent --- no added natural Siraitia grosvenorii syrup is all by monosaccharide from Siraitia grosvenorii and double Sugar and purified water composition, wherein glucide is mainly glucose, fructose, sucrose, mannitol.

Corresponding molecular structural formula is as follows:

It is domestic at present Siraitia grosvenorii and seedless monordica grosvenori are studied it is very much, research contents include corresponding Fructus Monordicae extract and Chemical component, but for Siraitia grosvenorii, generated absorption resin wastewater is further during production prepares Fructus Monordicae extract It utilizes, the research prepared containing glucose, fructose etc. containing sugared sweet ingredient is less.The factory of Current Domestic production Fructus Monordicae extract Family's majority is will to adsorb resin wastewater to be discharged into garden pipe network after Sewage Disposal is handled, and is not recycled.Main cause It is that absorption resin wastewater is substantially free of the main effective component such as momordica grosvenori glycoside V, majority is not a manufacturer deems that be worth；Or itself The glucides such as monosaccharide and the disaccharide for not thering is technology to go to recycle in absorption resin wastewater；Or without corresponding production equipment； Or since itself sales force is limited, there is not such product market, leads to the wasting of resources for adsorbing resin wastewater at this stage.

The absorption resin wastewater of Siraitia grosvenorii production process mainly contains the glucides such as glucose, fructose, sucrose, mannitol And protein, pigment etc., applicant of the present invention combine the production process of major product Fructus Monordicae extract, send out against expectation Having showed effectively to recycle glucose, fructose, sucrose, mannitol from absorption resin wastewater, obtained certain economic value Siraitia grosvenorii syrup, realize the comprehensive utilization of Siraitia grosvenorii, turn waste into wealth, create apparent economic and social benefit.

Summary of the invention

The technical problem to be solved by the present invention is to be overcome drawbacks described above of the existing technology, produced with fresh fructus momordicae The absorption resin wastewater of process is raw material, and large-scale production is prepared with the Siraitia grosvenorii syrup of certain economic value, realizes Siraitia grosvenorii Comprehensive utilization, turns waste into wealth, creates apparent economic and social benefit；The process route of a simple possible is looked for, life is reduced Cost is produced, working efficiency is improved.

The technical solution adopted by the present invention to solve the technical problems is:

The first purpose of this invention is to provide a kind of no added natural Siraitia grosvenorii syrup, be produced with fresh fructus momordicae The absorption resin wastewater of journey is made for raw material, specifically: it is glucose 14~24%, fructose 16~24%, sucrose 1~14%, sweet Reveal alcohol 1~8%, water 40~50%, remaining ingredient≤2%.It is preferred that glucose 14~22%, fructose 16~22%, sucrose 5~ 12%, mannitol 3~8%, water 40~50%, remaining ingredient≤1.5%.

Using the method for the present invention, other than Siraitia grosvenorii, seedless monordica grosvenori and water, any other additive is not added, is passed through Purification, depickling, goes agriculture residual and heavy metal at decoloration, concentration and etc. obtained Siraitia grosvenorii syrup it is relatively stable, after diluting It can directly drink, or as the component in food, drink, dairy products, health care product and drug etc..Without any additive, mouth Feel pure fragrant and sweet.

The component of the natural Siraitia grosvenorii syrup of the present invention is entirely from Siraitia grosvenorii, seedless monordica grosvenori or their any combination The pure natural product obtained, and product exists in liquid form.In process of production and in product, in addition to purified water, sieve Except Chinese fruit and seedless monordica grosvenori, without any additive, that is, a kind of solvent of purified water is only used.

For the natural Siraitia grosvenorii syrup with strong fruit flavor, pure in mouth feel and better than sucrose, sweet taste saturation degree is high.Institute State absorbance≤0.2 of the dilute aqueous solution 420nm at that natural Siraitia grosvenorii syrup solid content is 1%w/v, pol for 65~ 70brix。

Second object of the present invention is to provide the preparation method of above-mentioned no added natural Siraitia grosvenorii syrup, including as follows Step:

(1) purification of three-level UF membrane and concentration: the three-level film is respectively ultrafiltration membrane, nanofiltration membrane and reverse osmosis membrane；

(2) depickling: organic acid is removed with physical adsorption way with macroporous absorbent resin, obtains depickling liquid；

(3) physical decolorization and filtering；

(4) take off that agriculture is residual and heavy metal；

(5) it is concentrated.

Preferably, in step (1), the ultrafiltration retaining molecular weight is 2KD~3KD, the nanofiltration retaining molecular weight It is 500~800, the reverse osmosis membrane is to sodium chloride rejection > 99%.

Preferably, in step (1), the material of the ultrafiltration membrane is polyether sulfone, and the material of nanofiltration membrane and reverse osmosis membrane is poly- Amide.

It is further preferred that in step (1), 1.0~2.0MPa of operating pressure of the ultrafiltration membrane, nanofiltration membrane and reverse osmosis 2.0~3.0MPa of operating pressure of film.

The present invention removes big molecular impurity and pigment first with the macromolecule of ultrafiltration membrane, to reduce the osmotic pressure of film.Again The impurity and pigment for penetrating ultrafiltration membrane on a small quantity are removed, using the small-molecular-weight of nanofiltration membrane to improve purification and decolorizing effect.Finally Using rapid concentration under reverse osmosis membrane room temperature, to reduce concentrated cost and improve solid content.Preferably, using in the application The three-level membranous system of special parameter range can be extracted most effectively from waste liquid and obtain containing glucose, fructose, sucrose simultaneously With the syrup of mannitol, and the content of each component, in range appropriate, extraction efficiency is high, in good taste.

Preferably, in step (2), the macroporous absorbent resin includes styrene type, divinyl benzo-type, polyacrylate Type, acid/acrylic amide type macroporous absorbent resin, physically Adsorption organic acid and phenolic substances.Wherein, resin model is represented: LSA-7,LX-8,LX-17,XDA-8.Using the specific macroporous absorbent resin of the present invention in reverse osmosis membrane concentrate organic acid and Phenolic substances carries out physical absorption, and the glucides such as sucrose, glucose, fructose and mannitol are not adsorbed, with efflux one And flow out, to achieve the purpose that depickling.

It is further preferred that the granularity of the macroporous absorbent resin is 30~80 mesh.

It is further preferred that the amount of resin is related to the solid content of reverse osmosis membrane concentrate, that is, every The reverse osmosis membrane concentrate of 1000L pol 10Brix, with 120~200kg of resin.This parameter is to summarize and obtain through many experiments, According to the solid content in reverse osmosis membrane concentrate, the pH of macroporous absorbent resin efflux is repeatedly tested, until pH reaches 6 or so Subject to, such as 5-7, to calculate solid content in the macroporous absorbent resin and reverse osmosis membrane concentrate of special requirement model Relationship.

Preferably, in step (3), the Powdered Activated Carbon requirement: iodine sorption value >=900mg/g, Methylene blue adsorption ≥9mL.Specifications and models that there are many commercial powder active carbons need powder activity for pigment remaining in the depickling liquid of this patent Charcoal has higher iodine sorption value and stronger Methylene blue adsorption, to guarantee decolorizing effect.

It is further preferred that the dosage of the active carbon is related to the solid content of depickling liquid, i.e., in step (3) The depickling liquid of 1000L pol 10Brix, with 5.0~15.0kg of Powdered Activated Carbon.This parameter is obtained according to abundant experimental results, With this condition, by the physical decolorization mode of active carbon, pigment remaining in depickling liquid can be removed, is further improved Decolorizing effect.

Preferably, in step (4), the de- agriculture is residual, resin used in removing heavy-metal includes gel-type or macroporous type acrylic acid Resin, gel-type or macroporous type phenylethylene resin series.Represent resin model, it is residual to take off agriculture: LSA-900B, LSA-900D take off a huge sum of money Belong to: LX-500, LSC-As, LSC-400.The harmful substances such as the resin, heavy metal residual to agriculture of such special requirement model have compared with Good adsorption effect further promotes product quality to remove the harmful substances such as the residual, heavy metal of agriculture in destainer.

It is further preferred that in step (4), the de- agriculture is residual, the granularity of resin used in removing heavy-metal is 30~80 mesh.

It is further preferred that the amount of resin is related to the solid content of destainer, i.e. 1000L in step (4) The depickling liquid of pol 10Brix, with de- agriculture is residual and removing heavy-metal it is resin dedicated be 40~80kg.The nuisances such as the residual, heavy metal of agriculture Matter is eventually present in solid content, while being also scattered in solution with solid content.By testing in resin dedicated efflux Agriculture is residual, content of beary metal, can determine the relationship of the solid content of resin dedicated dosage and destainer.

Preferably, in step (5), it is described concentration include normal temperature condition under reverse osmosis membrane concentration with it is true under vacuum condition Sky concentration.It is most effective condensing mode that two kinds of concentration techniques, which are combined: reverse osmosis membrane is dense at a terrific speed at normal temperature Contracting, do not destroy ingredient and it is high-efficient, production cost is low, 10Brix can be concentrated into；After solid content is promoted to a certain degree, lead to Supreme solid content can be concentrated in a short time by crossing vacuum concentration, thus no added natural of the product-for obtaining this patent Siraitia grosvenorii syrup.

More specifically, the preparation method of no added natural Siraitia grosvenorii syrup provided by the invention, includes the following steps:

(1) purification of three-level UF membrane and concentration:

(1) the absorption resin wastewater of fresh fructus momordicae production process is pumped into organic membrane system and carries out the 1st purification and removal of impurities, Select ultrafiltration retaining molecular weight for 2KD~3KD, collection membrane downstream liquid, that is, refined liquid obtains ultrafiltration membrane refined liquid；

(2) ultrafiltration membrane refined liquid is pumped into organic membrane system and carries out the 2nd purification and decoloration, nanofiltration membrane is selected to retain molecule Amount is 500~800, and collection membrane downstream liquid, that is, refined liquid obtains nanofiltration membrane refined liquid；

(3) nanofiltration membrane refined liquid is pumped into reverse osmosis membrane system, film is concentrated into 5~10Brix, obtains reverse osmosis membrane concentrate；

(2) depickling: reverse osmosis membrane concentrate is pumped into depickling macroporous adsorbent resin special chromatography gravity tank, with the side of gravity flow Formula is by resin column, into after complete material, with 1~3 times of volume purifying washing column of resin column, collects into column efflux and water lotion, Obtain depickling liquid；

(3) physical decolorization and filtering: Powdered Activated Carbon being added into depickling liquid, is sufficiently stirred and is uniformly mixed under room temperature, 20~30min is kept, plate-frame filtering obtains the colourless destainer of clear；

(4) take off that agriculture is residual and heavy metal: destainer is residual resin dedicated and/or resin dedicated point of removing heavy-metal by de- agriculture Not Tuo Chu agriculture is residual and heavy metal, wash column into after complete material, then with the purifying of 2~3 times of resin volumes, will be remaining effective in column Ingredient washes out, and merges efflux and water lotion, obtains removing heavy-metal liquid；

(5) film concentration and vacuum concentration:

(1) reverse osmosis membrane is concentrated: removing heavy-metal liquid being concentrated into 5~10Brix by reverse osmosis membrane complete set of equipments, obtains film Concentrate；

(2) it is concentrated in vacuo: film concentrate is concentrated in vacuo, control Wen Du≤65 DEG C processed, very sky≤0.085MPa is concentrated into 60 ~70Brix obtains no added natural Siraitia grosvenorii syrup.

Third object of the present invention is to provide the purposes of the no added natural Siraitia grosvenorii syrup, which is characterized in that As the component in the product including food, drink, dairy products, health care product and drug.

The principle of the present invention is as follows:

The present invention mainly contains glucose, fruit using the absorption resin wastewater of fresh fructus momordicae production process as raw material in raw material The glucides such as sugar, sucrose, mannitol and protein, pigment etc., and the phenolic acid such as former flavones being present in fresh fructus momordicae Pigment, it is most of in the macroporous adsorption resin chromatography technique of faint yellow Momordia grosvenori aglycone production process by resin adsorption, thus Make seldom the presence of this kind of impurity and pigment in the raw material of this patent.

First raw material is refined and is concentrated by three-level UF membrane, is successively ultrafiltration membrane, nanofiltration membrane, reverse osmosis membrane, first with The macromolecule removal big molecular impurity and pigment of ultrafiltration membrane, to reduce the osmotic pressure of film；Recycle the small-molecular-weight of nanofiltration membrane Removal penetrates the impurity and pigment of ultrafiltration membrane on a small quantity, to improve purification and decolorizing effect；Finally using fast under reverse osmosis membrane room temperature Speed concentration, to reduce concentrated cost and improve solid content, to improve efficiency and late stage process effect.Reverse osmosis membrane concentrate In there are a small amount of organic acid and phenolic substancess, or part acids object is generated due to the glucide microbe conversion in production process Matter under the premise of not introducing foreign substance, is needed through the big of special requirement model under the premise of not influencing product special flavour Macroporous adsorbent resin is adsorbed these organic acids and phenolic substances with physical adsorption way, and sucrose, glucose, fructose and mannitol Equal glucides are not adsorbed and flow out from resin column, to be removed these organic acids and phenolic substances by physics mode. By the purification of UF membrane before and decoloration, still have minute quantity pigment through nanofiltration membrane, to obtain the product of high-quality, need with Physics mode removes these pigments, and satisfactory Powdered Activated Carbon can be by way of physical absorption by these pigments Absorption, then be aided with plate-frame filtering mode and remove the Powdered Activated Carbon for having adsorbed pigment, to obtain colourless destainer.Destainer In still have the harmful substances such as the residual, heavy metal of micro agriculture, need this kind of harmful substance through the resin of special requirement model Adsorption, to promote product quality.Finally by the reverse osmosis membrane concentration technique under room temperature in a manner of high-efficiency and low-cost The residual liquid with heavy metal of de- agriculture is further condensed into higher solid content, then by being concentrated in vacuo to consolidating for required specification Shape object content, to obtain the no added natural Siraitia grosvenorii syrup of this patent product-.

Beneficial effects of the present invention are as follows:

(1) using the absorption resin wastewater of fresh fructus momordicae production process as raw material, it is prepared for new product --- natural Siraitia grosvenorii Syrup.The syrup all by from Siraitia grosvenorii monosaccharide and disaccharide and purified water form, specifically: glucose 14~24%, Fructose 16~24%, sucrose 1~14%, mannitol 1~8%, water 40~50%, remaining ingredient≤2%.The syrup has strong Fruit flavor, pure in mouth feel and be better than sucrose, sweet taste saturation degree is high；Diluted aqueous solution (solid content 1%w/v) is in 420nm Absorbance≤0.2 at place.

(2) keep solution generation discoloration and muddy Siraitia grosvenorii protein and pigment etc. miscellaneous with the removal of three-level membrane separation technique Matter improves decoloration and purification efficiency；It does not use ion exchange resin to adjust the pH of solution, but uses the big of special requirement model Macroporous adsorbent resin does not introduce exogenous ion with organic acid and phenolic substances in physical adsorption way adsorbing separation removal solution Substance, to reduce the influence to product special flavour；Ion exchange resin etc. is not used to have the decoloration side centainly influenced to product special flavour Formula, and using three-level UF membrane and decolouring technology decoloration and physical decolorization mode mainly is combined, completely remove the color in solution Element；With the concentration of reverse osmosis membrane concentration technique combination vacuum concentration technology, thickening efficiency is improved, reduces production cost.

(3) comprehensive utilization for realizing Siraitia grosvenorii, while producing major product Momordia grosvenori aglycone, from macroporous absorbent resin layer Analyse separation and Extraction Siraitia grosvenorii syrup in waste water, further improve product category and added value, create it is apparent economical and Social benefit.

(4) solve the problems, such as that adsorption chromatography waste water seriously pollutes environment in Siraitia grosvenorii production process with the method for innovation. The adsorption chromatography waste water of Siraitia grosvenorii production process is turned waste into wealth, separation and Extraction carbohydrate content therein fundamentally solves The problem of COD and BOD high in waste water.

(5) administering method of novel related Siraitia grosvenorii adsorption chromatography waste water is established, and simple production process is feasible, if Standby simple, treatment cost is low, is suitble to promote.

Specific embodiment

Below with reference to embodiment, the invention will be further described.

Raw material used in the embodiment of the present invention, that is, fresh fructus momordicae production process absorption resin wastewater is raw from our company Produce workshop.Purified water used in the embodiment of the present invention is that our company's workshop is prepared with pure water equipment.The embodiment of the present invention Used raw and auxiliary material is obtained by routine business approach unless otherwise specified.

In the embodiment of the present invention, glucose, fructose, sucrose according under " Chinese Pharmacopoeia " 2015 editions " honey " items with efficient liquid Phase chromatography is detected with differential refraction detector, and mannitol is matched with high performance liquid chromatography according to " Chinese Pharmacopoeia " 2015 editions and shows difference Refraction detector detection.