阅读说明：本技术 大吉岭红茶提取物的应用 (application of red tea extract of Jieming ) 是由 姚期凤 姜丹 吴越 王越 蒋耀权 章漳 于 2018-05-29 设计创作，主要内容包括：本发明公开了一种大吉岭红茶提取物的应用,具体为在制备抗皮肤衰老基因的表达上调剂中的应用。所述的抗皮肤衰老基因可为ELN基因、FBN1基因、COL3A1基因、IGF1基因、CSF2基因、NID1基因和HSPG2基因中的一种或多种。所述的抗皮肤衰老基因的表达上调剂可以增强和支持皮肤组织结构的稳固性,延缓皮肤衰老,可用于化妆品或者护肤品中。(the invention discloses an application of a red tea extract of Guijiling mountains, in particular to an application in preparing an expression up-regulator of an anti-skin aging gene. The skin aging resistant gene can be one or more of ELN gene, FBN1 gene, COL3A1 gene, IGF1 gene, CSF2 gene, NID1 gene and HSPG2 gene. The expression up-regulator of the anti-skin aging gene can enhance and support the stability of the skin tissue structure and delay skin aging, and can be used in cosmetics or skin care products.)

1. An application of extract of Dajiling red tea (Fopflowry orange pekoe) in preparing up-regulator of the expression of anti-skin-aging gene is disclosed.

2. The use of claim 1, wherein: the anti-skin aging gene is one or more of ELN gene, FBN1 gene, COL3A1 gene, IGF1 gene, CSF2 gene, NID1 gene and HSPG2 gene.

3. The use of claim 1, wherein: the red tea extract is an aqueous extract of red tea.

4. The use of claim 1, wherein: the red tea extract is the red tea extract.

5. The use of claim 1, wherein: the preparation method of the red tea extract comprises the following steps:

(1) Adding water into the powder of Dajiling black tea for extraction, and filtering to obtain filtrate;

(2) Drying the filtrate.

6. The use of claim 5, wherein: the powder of the red tea is the powder of red tea;

And/or the mesh number of the powder of the red tea of Jijiling is 20-60 meshes;

And/or the addition amount of the water is 8-12 times of the volume of the powder of the red tea of Guiling;

and/or, the temperature of the extraction is 80-100 ℃;

and/or, the extraction times are more than 1;

And/or, the extraction time is 25-40 min;

and/or the filtration method is centrifugation;

And/or, the drying is freeze drying.

7. The use of claim 1, wherein: the preparation method of the red tea extract comprises the following steps:

(1) Adding 10 times of water into powder of Dajiling black tea, extracting in 90 deg.C water bath for 30min for 2 times, centrifuging, and mixing filtrates; the mesh number of the powder of the black tea leaves in Guijiling mountains is 20 meshes; the speed of the centrifugation is 8000rpm, and the time of the centrifugation is 10 minutes;

(2) And (5) freezing and drying the filtrate.

8. The use of claim 7, wherein: the concentration of the green tea extract in the expression up-regulator of the anti-skin aging gene is 8-120 mug/mL, and can also be 10-100 mug/mL.

9. The use of claim 8, wherein: the concentration of the green black tea extract in the expression up-regulator aiming at the ELN gene is 8-120 mug/mL, and can also be 10-100 mug/mL;

And/or the concentration of the green tea extract in an expression up-regulator aiming at one or more genes of FBN1 gene, IGF1 gene, CSF2 gene, NID1 gene and HSPG2 gene is 8-12 mu g/mL, and also 10 mu g/mL;

And/or the concentration of the green tea extract in the up-regulator for the expression of the COL3A1 gene is 80-120 mu g/mL, and can also be 100 mu g/mL.

10. Use according to any one of claims 1 to 9, wherein: the expression up-regulator of the anti-skin aging gene is used in cosmetics or skin care products.

Technical Field

The invention relates to the field of plant extracts and daily chemicals, and particularly relates to an application of a red tea extract from Jieming.

Background

The skin covers the surface of a human body and is in direct contact with the external environment, so that the human body can be protected from being damaged by various external factors, and the human body can be used as a mirror to reflect the health state of the human body. The skin is composed of epidermis, dermis, and subcutaneous tissue, and contains skin appendages, abundant nerves, blood vessels, lymphatic vessels, and muscles. The connective extracellular matrix region of the dermis and epidermis, also known as the Basement Membrane (BM), not only supports the epidermis, but also serves multiple functions, such as the junctions of the dermis and epidermis acting as a selective cellular barrier that generally prevents connective tissue cells from contacting the epidermal cells of the Basement membrane, but allows passage of lymphocytes, macrophages, and neuronal synapses; in addition, many vital phenomena such as cell morphology, cell polarity, cell metabolism, protein distribution in plasma membranes, cell survival, cell proliferation and differentiation, and cell migration are very closely related to the basement membrane. Nestin and perlecan are important components of basement membrane, essential for maintaining the structure and function of basement membrane, which is essential for maintaining normal renewal of skin. The dermis is composed of cells and extracellular matrix, and fibroblasts are main cells of the dermis, and the normal proliferation and differentiation of the fibroblasts maintain the structure of the dermis of the skin and form normal structure and physiological functions; extracellular matrix is a kind of macromolecular substance synthesized and secreted by cells and interacting around the cells, and many life phenomena such as proliferation and differentiation of cells, cell migration and the like are closely related to the extracellular matrix. The extracellular matrix is composed of four major classes of molecules: collagen, elastin, glycoprotein and proteoglycan. The extracellular matrix is finely and skillfully entangled and interwoven through different molecules to form various exquisite three-dimensional structures, so that the skin is endowed with elasticity and tensile strength, and looks plump and full of vitality.

The COL3a1(collagen type III alpha 1chain) gene encodes type III collagen, which encodes the pre- α 1chain of type III collagen, a fibrillar collagen, which is commonly associated with type I collagen, and can be found in the connective tissues of the extensible body such as skin, lung, uterus, intestine and vascular system, and the production of type III collagen helps to strengthen and support the firmness of the skin tissue structure, delaying skin aging.

Elastin ELN gene encodes elastin, one of the two components of elastic fiber, which is the major extracellular matrix protein, playing a very important role in maintaining skin elasticity and restoring force, and making skin flexible and elastic. During the formation of elastic tissue, tropoelastin crosslinks and aggregates with each other to form elastin. Elastin is an important component of skin composition, elastin contains only 5% of elastin in skin and is a key for stimulating the generation of skin collagen and elastic fibers, collagen accounting for 70% of skin determines the softness and fullness of skin, elastic fibers determine the tightening and elasticity of skin, and 90% of elastic fibers are composed of elastin, so that the quantity of elastin determines the elasticity of skin and whether wrinkles appear, and elastin is an important reason for skin aging.

Fibrillin 1 encoded by FBN1(Fibrillin 1) gene is secreted by fibroblasts of dermis into extracellular matrix, and can be used as structural component of calcium-binding microfibril to provide stress structure for elastic and inelastic connective tissues of whole body and mediate a series of cell and matrix interactions. Is an important component of elastic fibers in connective tissue, and in the process of formation of elastic tissue, fibrillin 1 provides a framework for storing tropoelastin to grow around mature elastic fibers. The reduction of skin elasticity is an important characteristic of skin aging, the expression level of fibrillin 1 is closely related to skin elasticity, and the reduction of the expression level of fibrillin 1 is also a key factor causing skin aging.

The protein encoded by the CSF2(colony stimulating factor 2) gene is colony stimulating factor 2, also known as granulocyte macrophage colony stimulating factor, is a broad-spectrum cytokine, and has a proliferation promoting effect on early hematopoietic progenitor cells. Mainly acts on myeloid progenitor cells, exerts biological effect by binding with high affinity receptors thereof, enables the cells to rapidly enter the cell cycle and differentiate towards the granulocyte lineage and macrophage lineage to finally become mature cells, can prolong the life of the mature cells, and enhances the functions of the mature cells. Colony stimulating factor 2 is an important cell longevity factor and is closely related to skin aging.

The protein coded by IGF1(insulin like growth factor 1) gene is insulin-like growth factor 1, is a group of polypeptide substances with growth promoting effect, and is mainly present in blood circulation. IGF mediates the stimulation of growth hormone, regulating tissue growth and development, and plays an important role in the regulation of muscle volume and strength, maintenance of body composition, and nutrient metabolism. The insulin-like growth factor 1 can promote the migration of keratinocytes and promote the re-epithelialization of wound surface, so as to delay skin aging.

NID1(Nidogen) gene encodes protein nestin 1, which is important protein of basement membrane and plays a role in connecting type iv collagen and laminin 5, and is essential for the structure and function of basement membrane. Whether the structure and function of the basement membrane are normal or not determines whether the skin can be normally renewed and differentiated, the nestin expression is reduced, the basement membrane structure is damaged, the proliferation and differentiation of epidermal cells are influenced, and the skin is aged.

The heparan sulfate glycoprotein hspg2(heparan sulfate proteolycan) gene encodes the perlecan protein (perlecan), which is a major component of basement membrane and consists of three long-chain glycosaminoglycan-linked core proteins. Perlecan protein is a multidomain proteoglycan that binds and crosslinks many extracellular matrix components and cell surface molecules. Previous studies have shown that this protein interacts with laminin, delipidated protein, type IV collagen, etc., and plays an important role in a variety of biological activities. Perlecan may also promote growth factor activity, thereby stimulating endothelial growth and regeneration. The expression up-regulation of the hspg2 gene can promote the expression of perlecan protein, further promote the stability of a basement membrane structure, strengthen the tight connection of true epidermis and delay skin aging.

Therefore, the research on how to improve the expression of genes related to key parts and functions of the skin has important significance for improving the stability of the true epidermal structure of the skin and delaying skin aging.

Red tea (Fopflowry orange pekoe) is a small leaf-seed tea tree, growing in the south of himalayas, in the town of jiling, western india, very close to the mouth of the open port bank of chinese tibet. The Jiejing Ling is also called as "Jingangzhi", is a small city of West Bengal Bang in India, is located at the foot of Himalaya, has an average altitude of 2134 meters, is a plateau area, has steep terrain slope, cool climate, no environmental pollution and unique ecological environment, and ensures that the Jiejing Ling tea has elegant musk wine flavor and fantastic flower and fruit fragrance. The red tea in Dajiling mountain has the advantages of tight and thin appearance, obvious pekoe, high fragrance, strong taste and fresh and cool taste. The fermentation degree reaches about 80%, the tea leaves picked for the first time are green, and the tea leaves picked for the second time are brown. The aroma belongs to high aroma class, is called muscadine aroma tea, has relatively long-lasting aroma and sweet and soft taste. The soup color is clear and bright, the soup color is orange, yellow and red, and is pleasing to the eyes and is praised as 'champagne in tea' by the world. Picking tea in four seasons, wherein tea is picked for the first time in 3-4 months, tea is picked for the second time in 5-6 months, tea in rainy season in 7-8 months, and tea in autumn in 9-10 months. The primary picked tea is like the Chinese Ming-Qian tea and is regarded as a treasure; the tea picked for the second time has good fragrance and more remarkable taste, and both the tea and the tea are very popular with consumers.

The black tea can help gastrointestinal digestion, promote appetite, promote urination, eliminate edema and strengthen heart function. The flavonoids contained in black tea can eliminate free radicals, and has antacid effect, and can reduce incidence of myocardial infarction. The traditional Chinese medicine holds that the tea is divided into cold and hot tea, and the black tea and the Pu' er tea are warm, so that the tea is suitable for drinking in winter. The polyphenol compounds in black tea have anti-inflammatory effect, and experiments show that catechins can be combined with unicellular bacteria to coagulate and precipitate proteins, thereby inhibiting and eliminating pathogenic bacteria.

Disclosure of Invention

The invention aims to solve the technical problem of providing the application of a red tea extract of Jigeling, in particular to the application of the red tea extract in preparing an expression up-regulator of an anti-skin aging gene. The expression up-regulator of the anti-skin aging gene can enhance and support the stability of the skin tissue structure and delay skin aging, and can be used in cosmetics or skin care products.

The invention provides an application of a red tea extract of Jieming in preparing an expression up-regulator of an anti-skin-aging gene.

The skin aging resistant gene can be one or more of ELN gene, FBN1 gene, COL3A1 gene, IGF1 gene, CSF2 gene, NID1 gene and HSPG2 gene.

the red tea extract can be a red tea extract of red tea in the field, and can also be an aqueous extract of red tea in red tea.

the red tea extract can be the extract of red tea.

The preparation method of the red tea extract of green tea can be a conventional method in the field, and can also comprise the following steps:

(1) Adding water into the powder of Dajiling black tea for extraction, and filtering to obtain filtrate;

(2) drying the filtrate.

In the step (1), the powder of red tea from Jiejing mountain can be the powder of red tea from Jiejing mountain.

In the step (1), the mesh number of the black tea powder of Jijiling mountain can be 20-60 meshes, and can also be 20 meshes.

In the step (1), the addition amount of the water can be 8-12 times of the volume of the powder of the green tea, and can also be 10 times.

In the step (1), the extraction temperature can be 80-100 ℃ and can also be 90 ℃.

In the step (1), the number of times of extraction may be 1 or more, or may be 2.

In the step (1), the extraction time can be 25-40min, and can also be 30 min.

In step (1), the filtration method may be centrifugation. The speed of centrifugation can be 6000-10000rpm, and can also be 8000 rpm. The centrifugation time can be 8-12 minutes, and can also be 10 minutes.

In the step (2), the drying may be freeze drying.

the preparation method of the red tea extract can also comprise the following steps:

(1) adding 10 times of water into powder of Dajiling black tea, extracting in 90 deg.C water bath for 30min for 2 times, centrifuging, and mixing filtrates; the mesh number of the powder of the black tea leaves in Guijiling mountains is 20 meshes; the speed of the centrifugation is 8000rpm, and the time of the centrifugation is 10 minutes;

(2) And (5) freezing and drying the filtrate.

The concentration of the green tea extract in the expression up-regulator of the anti-skin aging gene can be 8-120 mu g/mL, and can also be 10-100 mu g/mL.

The concentration of the green black tea extract in the expression up-regulator aiming at the ELN gene can be 8-120 mu g/mL, and can also be 10-100 mu g/mL.

The concentration of the green tea extract in an expression up-regulator aiming at one or more genes of FBN1 gene, IGF1 gene, CSF2 gene, NID1 gene and HSPG2 gene can be 8-12 mu g/mL, and can also be 10 mu g/mL.

The concentration of the green tea extract in the up-regulator for the expression of the COL3A1 gene can be 80-120 mu g/mL, and can also be 100 mu g/mL.

wherein, the expression up-regulator of the anti-skin aging gene can be used in cosmetics or skin care products.

The red tea extract of Dajiling can be used in cosmetics or skin care products.

On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.

The reagents and starting materials used in the present invention are commercially available.

The positive progress effects of the invention are as follows: the application of the black tea extract of Dajiling mountain is provided, in particular to the application in preparing an expression up-regulator of a skin aging resistant gene, the expression up-regulator of the skin aging resistant gene can enhance and support the stability of a skin tissue structure and delay skin aging, and can be used in cosmetics or skin care products.

Drawings

FIG. 1 is a graph showing the results of ELN gene expression promotion by extract of black tea of Guillain 10. mu.g/mL.

FIG. 2 is a graph showing the results of 10. mu.g/mL Guillain black tea extract promoting the expression of FBN1 gene.

FIG. 3 is a graph showing the results of ELN gene expression promotion by 100. mu.g/mL Guillain black tea extract.

FIG. 4 is a graph showing the results of promoting COL3A1 gene expression by 100. mu.g/mL Guillain black tea extract.

FIG. 5 is a graph showing the results of 10. mu.g/mL Guillain black tea extract promoting IGF1 gene expression.

FIG. 6 is a graph showing the results of 10. mu.g/mL Guillain black tea extract promoting CSF2 gene expression.

FIG. 7 is a graph showing the results of promoting NID1 gene expression by 10. mu.g/mL Guillain black tea extract.

FIG. 8 is a graph showing the results of 10. mu.g/mL of Guillain black tea extract promoting expression of HSPG2 gene.

Detailed Description

The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.