阅读说明：本技术 一种从枳椇子中提取总黄酮的制备方法 (preparation method for extracting total flavonoids from hovenia dulcis thunb ) 是由 杨成东 于 2018-05-29 设计创作，主要内容包括：本发明公开了一种从枳椇子中提取总黄酮的制备方法,其特征在于以下步骤：1)取干燥枳椇子叶,研磨制成粗粉；2)取粗粉,加入75%乙醇25ml,回流提取2次,每次80mL,每次1~3h,合并提取液,回收乙醇,得到枳椇子浸膏；3)提取：取枳椇子浸膏加入适量蒸馏水,搅匀,加入到层析柱中调节流速为5ml/min,用蒸馏水洗去杂质,以10~40%乙醇为流动相解吸附；4)回收乙醇,得总黄酮提取物,真空干燥即得总黄酮。本发明制备的总黄酮,产品纯度高,品质好,工艺方法简便易行,适用于工业化生产。(The invention discloses a preparation method for extracting total flavonoids from hovenia dulcis thunb, which is characterized by comprising the following steps of: 1) grinding dried folium Hoveniae into coarse powder; 2) taking the coarse powder, adding 25mL of 75% ethanol, performing reflux extraction for 2 times, each time 80mL, each time for 1-3 h, combining the extracting solutions, and recovering ethanol to obtain hovenia dulcis thunb extract; 3) extraction: adding a proper amount of distilled water into the hovenia dulcis thunb extract, uniformly stirring, adding the mixture into a chromatographic column, adjusting the flow rate to be 5ml/min, washing the mixture with distilled water to remove impurities, and desorbing the mixture by using 10-40% ethanol as a mobile phase; 4) recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone. The total flavone prepared by the method has high product purity, good quality, simple and easy process method, and is suitable for industrial production.)

1. A preparation method for extracting total flavonoids from hovenia dulcis thunb is characterized by comprising the following steps:

1) grinding dried semen Hoveniae into coarse powder;

2) taking the coarse powder, adding 25mL of 75% ethanol, performing reflux extraction for 2 times, each time 80mL, each time for 1-3 h, combining the extracting solutions, and recovering ethanol to obtain hovenia dulcis thunb extract;

3) Extraction: adding a proper amount of distilled water into the hovenia dulcis thunb extract, uniformly stirring, adding the mixture into a chromatographic column, adjusting the flow rate to be 5ml/min, washing the mixture with distilled water to remove impurities, and desorbing the mixture by using 10-40% ethanol as a mobile phase;

4) Recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone.

2. the method for preparing total flavonoids from hovenia dulcis thunb according to claim 1, wherein the reflux extraction time in the step 2) is 1-3 h.

3. The preparation method for extracting total flavonoids from hovenia dulcis thunb according to claim 1, which is characterized in that the chromatographic column in the step 3) is prepared by pretreating D101 type macroporous resin and polyamide, and the content of mobile phase-desorbed ethanol is 10-40%.

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of total flavonoids.

Background

Hovenia acerba is a species of Hovenia of Rhamnaceae. Hovenia dulcis, belonging to Rhamnaceae Hovenia, is a deciduous broad-leaf tall tree with the tree height of up to 30 meters, namely Hovenia dulcis, Crataegus pinnatifida, papaw, sarcocarp, orange twister, Crataegus pinnatifida, and Crataegus pinnatifida, wherein most provinces of China are sporadically distributed and wild. Has medical value, and can clear heat, promote urination, relieve cough, relieve restlessness and relieve alcoholism. Its skin has the functions of promoting blood circulation, relaxing muscles and tendons and detoxicating. Can be used for treating gastrocnemius spasm, food stagnation, and hammer poisoning. The fruit stalks can invigorate the stomach and enrich the blood.

The hovenia dulcis thunb alcohol extract can improve the oxidation function of reduced Glutathione (GSH) of mice with acute liver injury, enhance GSH binding reaction catalyzed by glutathione S-transferase (GST), increase the activities of superoxide dismutase (SOD) and catalase (Cat), and antagonize the mice with acute liver injury caused by ethanol. The 75% ethanol extract of the hovenia dulcis thunb has definite anti-hepatic fibrosis effect. The flavone compounds containing quercetin and the like are separated from hovenia dulcis thunb and possibly are the effective components of the flavone compounds.

the flavonoids generally refer to a series of compounds in which two benzene rings (A-and B-rings) having phenolic hydroxyl groups are connected with each other through a central three-carbon atom, and the basic nucleus of the compounds is 2-phenylchromone. The structure of the flavonoid compound is often connected with functional groups such as phenolic hydroxyl, methoxyl, methyl, isopentenyl and the like. In addition, it is often combined with a sugar to form a glycoside. The total flavonoids compounds in the hovenia dulcis thunb have the obvious functions of resisting oxidation and free radicals, resisting and preventing cancers, lowering blood pressure, regulating blood fat, improving microcirculation, inhibiting bacteria, diminishing inflammation, easing pain and the like.

Disclosure of Invention

the invention aims to provide a method for extracting total flavonoids, and the total flavonoids have wide medicinal and health-care values.

The invention is completed by the following technical scheme, and the specific content comprises the following steps: method for extracting total flavone

1) Grinding dried semen Hoveniae into coarse powder;

2) taking the coarse powder, adding 25mL of 75% ethanol, performing reflux extraction for 2 times, each time 80mL, each time for 1-3 h, combining the extracting solutions, and recovering ethanol to obtain hovenia dulcis thunb extract;

3) Extraction: adding a proper amount of distilled water into the hovenia dulcis thunb extract, uniformly stirring, adding the mixture into a chromatographic column, adjusting the flow rate to be 5ml/min, washing the mixture with distilled water to remove impurities, and desorbing the mixture by using 10-40% ethanol as a mobile phase;

4) Recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone.

The reflux extraction time in the step 2) is 1-3 h.

The chromatographic column in the step 3) is prepared by pretreating D101 type macroporous resin and polyamide, and the content of the mobile phase desorption ethanol is 10-40%.

the invention has the advantages that

The invention adopts ultrasonic extraction-D101 type macroporous resin and polyamide column chromatography to extract the total flavone in the hovenia dulcis thunb, the sample capacity of the column chromatography is large, the extraction efficiency is high, and the method is simple and easy to implement.

the present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

The specific implementation mode is as follows: