阅读说明：本技术 马甲子或其提取物在制备抗肿瘤转移药物上的用途 (application of paliurus ramosissimus or extract thereof in preparation of anti-tumor metastasis medicaments ) 是由 李东晓 周静 徐超群 于 2019-09-29 设计创作，主要内容包括：本发明提供了马甲子或其提取物在制备抗肿瘤转移药物上的用途。特别地,所述马甲子提取物为马甲子鲜叶的乙醇提取物或乙酸乙酯提取物。实验证明,马甲子提取物对肿瘤细胞迁移有明显抑制作用,在制备抗肿瘤转移的药物上具有很好的应用前景。(The invention provides an application of malachite seed or an extract thereof in preparing a medicine for resisting tumor metastasis. In particular, the paliurus ramosissimus extract is an ethanol extract or an ethyl acetate extract of fresh paliurus ramosissimus leaves. Experiments prove that the paliurus ramosissimus extract has obvious inhibition effect on tumor cell migration and has good application prospect in preparing anti-tumor metastasis medicaments.)

1. Use of paliurus ramosissimus or its extract in preparing medicine for resisting tumor metastasis is provided.

2. Use according to claim 1, characterized in that: the tumor is a malignant tumor.

3. Use according to claim 2, characterized in that: the tumor is gastric cancer, cecum cancer or colorectal cancer.

4. Use according to any one of claims 1 to 3, characterized in that: the semen Strychni extract is semen Strychni organic solvent extract.

5. Use according to claim 4, characterized in that: the organic solvent extract of the paliurus ramosissimus is an ethanol extract or an ethyl acetate extract of the paliurus ramosissimus.

6. Use according to any one of claims 1 to 3, characterized in that: the paliurus ramosissimus is fresh leaf of paliurus ramosissimus or dry leaf of paliurus ramosissimus.

7. Use according to claim 6, characterized in that: the dry leaves of the paliurus ramosissimus are products obtained by extracting fresh leaves of the paliurus ramosissimus with a solvent and drying; or, freeze-drying the obtained product of the fresh leaves of the paliurus ramosissimus; or naturally drying the obtained product of the fresh leaves of the paliurus ramosissimus.

8. Use according to any one of claims 1 to 7, characterized in that: the preparation method of the paliurus ramosissimus extract comprises the following steps: pulverizing squama Manis, extracting with organic solvent to obtain extractive solution a, and drying.

9. Use according to claim 8, characterized in that: the extraction mode is reflux, cold soaking or percolation extraction;

The organic solvent is one or two of ethanol and ethyl acetate; preferably, the ethanol concentration is 80% or more, more preferably 95%;

Therefore, the weight of the organic solvent is 5-10 times of that of the malachite.

10. Use according to claim 8 or 9, characterized in that: the preparation method of the paliurus ramosissimus extract comprises the following specific steps:

The method comprises the following steps: pulverizing semen Strychni, extracting with ethanol to obtain extractive solution 1, and drying to obtain semen Strychni ethanol extract 1;

Or, method 2: taking the ethanol extract 1 of the paliurus ramosissimus prepared by the method 1, adding ethyl acetate for extraction to obtain an extracting solution 2, and drying to obtain an ethyl acetate extract 2 of the paliurus ramosissimus;

Or, method 3: taking the extracting solution 1 obtained in the method 1, adding water, adding petroleum ether for extraction, adding ethyl acetate into the water phase for extraction to obtain an extracting solution 3, and drying to obtain an ethyl acetate extract 3 of the paliurus ramosissimus.

Technical Field

The invention relates to the field of medicines, in particular to application of malachite fruit or an extract thereof in preparing a medicine for resisting tumor metastasis.

Background

Tumor metastasis is the main mode of tumor spread, can spread tumor cells to multiple systems or organs of a human body, and is an important cause of tumor aggravation or death. Tumors are divided into benign tumors and malignant tumors, and one of the most important characteristics of malignant tumor cells is metastasis, i.e., metastasis from a primary tumor to other parts forms a metastatic tumor, and the growth of a recurrent tumor is maintained, which is the first factor of death of cancer patients caused by tumor metastasis.

Only a small fraction of cells in primary tumors have metastatic potential. The metastasis of tumor is different from the mechanism of tumorigenesis, is a multi-stage process of multi-factor and multi-gene mutual coordination, is a very complex process, and comprises a series of cascade reactions including the steps that tumor cells are separated from primary focuses, invade extracellular matrixes, invade nearby blood vessels and lymphatic vessels to enter a circulatory system, adhere to blood platelets and endothelial cells at target spots, interact with the blood platelets and the endothelial cells at the target spots to penetrate out of a vascular system, form new clones at the target spots, and effectively escape from immune clearance and other events of an organism.

Researches find that most of anti-tumor drugs can not inhibit tumor metastasis, and no good treatment method for tumor metastasis exists at present.

therefore, the search for drugs capable of inhibiting tumor metastasis is the key research content of the current tumor treatment, and has wide prospects.

Disclosure of Invention

The invention aims to provide a new application of malachite fruit or an extract thereof in preparing a medicine for resisting tumor metastasis.

The invention provides an application of malachite seed or an extract thereof in preparing a medicine for resisting tumor metastasis.

Further, the tumor is a malignant tumor.

Further, the tumor is gastric cancer, cecum cancer or colorectal cancer.

further, the paliurus ramosissimus extract is an organic solvent extract of paliurus ramosissimus.

Further, the organic solvent extract of the paliurus ramosissimus is an ethanol extract or an ethyl acetate extract of the paliurus ramosissimus.

further, the paliurus ramosissimus is fresh leaves or dry leaves of paliurus ramosissimus.

Further, the dry leaves of the paliurus ramosissimus are products obtained by extracting fresh leaves of the paliurus ramosissimus with a solvent and drying; or, freeze-drying the obtained product of the fresh leaves of the paliurus ramosissimus; or naturally drying the obtained product of the fresh leaves of the paliurus ramosissimus.

Further, the preparation method of the paliurus ramosissimus extract comprises the following steps: pulverizing squama Manis, extracting with organic solvent to obtain extractive solution a, and drying.

Further, the extraction mode is reflux, cold soaking or percolation extraction;

The organic solvent is one or two of ethanol and ethyl acetate; preferably, the ethanol concentration is 80% or more, more preferably 95%;

therefore, the weight of the organic solvent is 5-10 times of that of the malachite.

Further, the preparation method of the paliurus ramosissimus extract comprises the following specific steps:

The method comprises the following steps: pulverizing semen Strychni, extracting with ethanol to obtain extractive solution 1, and drying to obtain semen Strychni ethanol extract 1;

Or, method 2: taking the ethanol extract 1 of the paliurus ramosissimus prepared by the method 1, adding ethyl acetate for extraction to obtain an extracting solution 2, and drying to obtain an ethyl acetate extract 2 of the paliurus ramosissimus;

Or, method 3: taking the extracting solution 1 obtained in the method 1, adding water, adding petroleum ether for extraction, adding ethyl acetate into the water phase for extraction to obtain an extracting solution 3, and drying to obtain an ethyl acetate extract 3 of the paliurus ramosissimus.

The paliurus ramosissimus is a common medicinal plant, is reported to be both medicinal in branches, leaves, roots, flowers and fruits, is recorded in the folk of Chinese medicine dictionary and some local medicinal ideas, and is taken by patients with tumors for a long time in folk to obtain satisfactory treatment effects. However, there is no report that the malachite can be used for resisting tumor metastasis.

The percolation extraction is a method of placing the moderately crushed medicinal materials in a percolation cylinder, continuously adding a solvent from the upper part, and leaching out medicinal material components in the process of the solvent seeping through a medicinal material layer and flowing downwards.

The invention proves that the paliurus ramosissimus or the extract thereof, particularly the ethanol extract and the ethyl acetate extract of the fresh paliurus ramosissimus leaves have obvious inhibition effect on tumor cell migration, and have good application prospect in preparing the anti-tumor metastasis medicament.

Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.

The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.

Detailed Description

The raw materials and instruments used in the present invention are known products, and are obtained by purchasing commercially available products.