阅读说明：本技术 一种采集空气中微生物的装置及其采集方法 (Device and method for collecting microorganisms in air ) 是由 陈晖� 潘亮 于 2021-08-09 设计创作，主要内容包括：本发明涉及空气环境监测技术领域,尤其为一种采集空气中微生物的装置及其采集方法,包括底座、加热池、蠕动泵、冷凝增长池和气泵,所述蠕动泵的右侧连通有入管,所述加热池内腔的底部安装有若干个加热偶,所述加热池的顶部连通有蒸汽输送管,所述蒸汽输送管的表面安装有阀门；本发明采用增加空气的湿度再降温的方式达到过饱和状态,促使空气中的微生物粒径增长,并在经过小孔时随惯性撞击在采集器上,从而完成对微生物的高效采集,且该装置具备设计简单、成本低廉、使用方便的优点,解决了目前对空气中微生物的采集,大都采用平板法,传统的平板法仅能够采集到可沉降微生物,且效率低下的问题。(The invention relates to the technical field of air environment monitoring, in particular to a device for collecting microorganisms in air and a collection method thereof, wherein the device comprises a base, a heating pool, a peristaltic pump, a condensation growth pool and an air pump, wherein the right side of the peristaltic pump is communicated with an inlet pipe, the bottom of an inner cavity of the heating pool is provided with a plurality of heating couples, the top of the heating pool is communicated with a steam conveying pipe, and the surface of the steam conveying pipe is provided with a valve; the device disclosed by the invention achieves a supersaturated state by increasing the humidity of air and then cooling, promotes the particle size of microorganisms in the air to increase, and impacts a collector along with inertia when passing through a small hole, so that the microorganisms are efficiently collected.)

1. The utility model provides a gather device of microorganism in air, includes base (1), heating pond (2), peristaltic pump (3), condensation growth pond (4) and air pump (5), its characterized in that: the right side of the peristaltic pump (3) is communicated with an inlet pipe (6), the bottom of an inner cavity of the heating pool (2) is provided with a plurality of heating couples (7), the top of the heating pool (2) is communicated with a steam conveying pipe (8), the surface of the steam conveying pipe (8) is provided with a valve (9), one side of the air pump (5) is communicated with a gas conveying pipe (10), the other side of the air pump (5) is communicated with a collection port (11), the other ends of the steam conveying pipe (8) and the gas conveying pipe (10) are both communicated with the condensation growth pool (4), the bottom of the condensation growth pool (4) is communicated with a vertical pipe (12), the bottom of the vertical pipe (12) is communicated with a collection box (13), the front of the collection box (13) is hinged with a box door (26), a transverse plate (14) is arranged above the inner cavity of the collection box (13), and a small hole (15) is formed in the surface of the transverse plate (14) in a penetrating manner, the inside of collection box (13) and the below that is located diaphragm (14) are provided with striking plate (16), the top of striking plate (16) sets up carbon film copper mesh (17), the surface of condensation growth pond (4) is provided with copper pipe (18), the both ends of copper pipe (18) communicate respectively has inlet tube (19) and outlet pipe (20), the surface of inlet tube (19) and outlet pipe (20) is provided with water delivery control mechanism (21), the condensation growth pond (4) below on surface is provided with supporting mechanism (22).

2. The apparatus for collecting airborne microorganisms of claim 1, wherein: the water delivery control mechanism (21) comprises a water pump (211), a first electromagnetic valve (212) and a second electromagnetic valve (213), the water pump (211) and the first electromagnetic valve (212) are both installed on the surface of the water inlet pipe (19), and the second electromagnetic valve (213) is installed on the surface of the water outlet pipe (20).

3. The apparatus for collecting airborne microorganisms of claim 1, wherein: the supporting mechanism (22) comprises a fixing ring (221), a supporting rod (222) and a supporting plate (223), the fixing ring (221) is bolted below the surface of the condensation growth tank (4), one end of the supporting rod (222) is bolted with the fixing ring (221), the supporting plate (223) is bolted at the other end of the supporting rod (222), and the supporting plate (223) is bolted with the base (1).

4. The apparatus for collecting airborne microorganisms of claim 1, wherein: both sides on the surface of the impact plate (16) are penetrated and provided with air holes (27), the right side of the collection box (13) is communicated with an exhaust channel (23), and a fan (24) is arranged inside the exhaust channel (23).

5. The apparatus for collecting airborne microorganisms of claim 1, wherein: the surface bolt of condensation growth pond (4) has a plurality of mounting (25), copper pipe (18) are the heliciform, and copper pipe (18) are fixed mutually with mounting (25), the material that condensation growth pond (4) is the stainless steel.

6. The apparatus for collecting airborne microorganisms of claim 1, wherein: the aperture of the small holes (15) is 0.02-0.1 μm, the distance between the small holes (15) and the impact plate (16) is 0.2-5mm, the inner diameter of the condensation growth pool (4) is 50-80mm, and the length of the condensation growth pool (4) is 300-600 mm.

7. A collection method of a device for collecting microorganisms in air is characterized in that: the acquisition method comprises the following steps:

step A: the inlet pipe (6) is externally connected with a pure water source, ultrapure water is introduced into the heating pool (2) at the flow rate of 0.5-2ml/min by using the peristaltic pump (3), the heating couple (7) is started to heat the ultrapure water to raise the temperature, the ultrapure water is heated to 100 ℃ to generate steam, and the valve (9) is opened to enable the steam to enter the interior of the condensation growth pool (4) through the steam delivery pipe (8);

and B: starting the air pump (5), enabling outside air and microorganisms to enter the condensation growth tank (4) through the acquisition port (11), the air pump (5) and the air pipe (10), and enabling the air entering the condensation growth tank (4) to be in contact with water vapor at the moment, so that the humidity and the temperature of the air are increased;

and C: the water pump (211) and the first electromagnetic valve (212) are started, cooling water with lower temperature is put into the water inlet pipe (19), at the moment, the cooling water enters the copper pipe (18) through the water inlet pipe (19), then, heat in the air is transferred to the copper pipe (18) through the condensation growth pool (4), the cooling water in the copper pipe (18) absorbs heat, and the temperature of the air in the condensation growth pool (4) is greatly reduced;

step D: the microorganisms reach an oversaturated state under the condition that the humidity of the air is increased and the temperature is reduced, the particle size of the microorganisms in the air is increased, then the microorganisms pass through the small holes (15) along with the air flow and collide with the carbon film copper net (17) on the surface of the impact plate (16) under the action of inertia, so that the collection and the capture of the microorganisms are completed, and meanwhile, the air is discharged through the air holes (27) and the exhaust channel (23).

8. The method for collecting microorganisms in the air of claim 7, wherein: in the step A, the conductivity of the ultrapure water is more than or equal to 18 mu omega.

9. The method for collecting microorganisms in the air of claim 7, wherein: in the step B, the flow rate of the gas introduced by the gas pump (5) is 500-.

10. The method for collecting microorganisms in the air of claim 7, wherein: in the step C, the temperature of the cooling water is 0-15 ℃.

Technical Field

The invention relates to the technical field of air environment monitoring, in particular to a device for collecting microorganisms in air and a collection method thereof.

Background

Scientists have demonstrated that bioaerosols are one of the major transmission pathways for the new coronavirus outbreak. When human beings face similar known and unknown viruses, the detection of bioaerosols such as viruses, bacteria and microorganisms in the air is very necessary. The particle size range of microorganisms in air is wide, from tens of nanometers to tens of micrometers. The traditional flat plate method can only collect settleable microorganisms (more than several microns) and has low efficiency. In order to rapidly and reliably collect microorganisms in the air in the whole particle size range, particularly ultrafine microorganisms in the air with particle sizes of tens of nanometers, a special device needs to be designed for sampling.

Disclosure of Invention

The invention aims to provide a device for collecting microorganisms in air, which has the advantages of simple design, low cost and convenient use, and can efficiently collect the microorganisms in the air for further research.

In order to achieve the purpose, the invention provides the following technical scheme: a device for collecting microorganisms in air comprises a base, a heating pool, a peristaltic pump, a condensation growth pool and an air pump, wherein the right side of the peristaltic pump is communicated with an inlet pipe, a plurality of heating couples are installed at the bottom of an inner cavity of the heating pool, the top of the heating pool is communicated with a steam conveying pipe, a valve is installed on the surface of the steam conveying pipe, one side of the air pump is communicated with a gas conveying pipe, the other side of the air pump is communicated with a collection port, the other ends of the steam conveying pipe and the gas conveying pipe are both communicated with the condensation growth pool, the bottom of the condensation growth pool is communicated with a vertical pipe, the bottom of the vertical pipe is communicated with a collection box, the front of the collection box is hinged with a box door, a transverse plate is arranged above the inner cavity of the collection box, small holes are formed in the surface of the transverse plate in a penetrating manner, and a striking plate is arranged in the interior of the collection box and below the transverse plate, the top of striking board sets up carbon film copper mesh, the condensation increases the surface in pond and is provided with the copper pipe, the both ends of copper pipe communicate respectively has inlet tube and outlet pipe, the surface of inlet tube and outlet pipe is provided with water delivery control mechanism, the condensation increases the below on pond surface and is provided with supporting mechanism.

For can control the transport of cooling water, preferably, water delivery control mechanism includes water pump, first solenoid valve and second solenoid valve, water pump and first solenoid valve are all installed on the surface of inlet tube, the surface at the outlet pipe is installed to the second solenoid valve.

In order to support and fix the condensation growth tank in an auxiliary manner, it is preferable that the support mechanism includes a fixing ring, a support rod and a support plate, the fixing ring is bolted below the surface of the condensation growth tank, one end of the support rod is bolted with the fixing ring, the support plate is bolted at the other end of the support rod, and the support plate is bolted with the base.

In order to discharge air out of the device, preferably, air holes are formed in two sides of the surface of the impact plate in a penetrating mode, the right side of the collecting box is communicated with an exhaust channel, and a fan is arranged inside the exhaust channel.

In order to be able to assist and fix the copper pipe, preferably, the surface of the condensation growth pool is bolted with a plurality of fixing pieces, the copper pipe is in a spiral shape and is fixed with the fixing pieces, and the condensation growth pool is made of stainless steel.

In order to ensure that air can flow between the impact plate and the transverse plate, the diameter of the small holes is preferably 0.02-0.1 μm, the distance between the small holes and the impact plate is preferably 0.2-5mm, the inner diameter of the condensation growth tank is preferably 50-80mm, and the length of the condensation growth tank is preferably 300-600 mm.

A collection method of a device for collecting microorganisms in air comprises the following steps:

step A: connecting a pure water source outside an inlet pipe, introducing ultrapure water into a heating pool at the flow rate of 0.5-2ml/min by using a peristaltic pump, starting a heating couple to heat the ultrapure water to raise the temperature, heating to 100 ℃ to generate steam, and starting a valve to enable the steam to enter the interior of a condensation growth pool through a steam delivery pipe;

and B: starting an air pump, enabling outside air and microorganisms to enter the condensation growth tank through a collection port, the air pump and an air delivery pipe, and enabling the air entering the condensation growth tank to be in contact with water vapor at the moment, so that the humidity and the temperature of the air are increased;

and C: the water pump and the first electromagnetic valve are started, cooling water with lower temperature is put into the water inlet pipe, the cooling water enters the copper pipe through the water inlet pipe at the moment, then heat in the air is transferred to the copper pipe through the condensation growth pool, the cooling water in the copper pipe absorbs heat, and the temperature of the air in the condensation growth pool is greatly reduced;

step D: the microorganism reaches the oversaturation state under the condition that the humidity of air increases and cools down again, and the microorganism particle size in the air increases, and later the microorganism bumps into the carbon film copper mesh on striking plate surface under the effect of inertia along with the air current passes through the aperture to accomplish the collection of microorganism and catch, the air is discharged through bleeder vent and exhaust passage simultaneously.

In order to ensure the purity of water, it is preferable that the conductivity of the ultrapure water introduced in the step A is not less than 18 μm Ω.

In order to control the flow rate of air, it is preferable that the flow rate of the gas introduced by the gas pump in step B is 500-.

In order to secure the cooling effect on the air, it is preferable that the temperature of the cooling water in the step C is 0 to 15 ℃.

Compared with the prior art, the invention has the following beneficial effects:

the device disclosed by the invention achieves a supersaturated state by increasing the humidity of air and then cooling, promotes the particle size of microorganisms in the air to increase, and impacts a collector along with inertia when passing through a small hole, so that the microorganisms are efficiently collected.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic perspective view of the present invention;

FIG. 3 is a front sectional view of the structure of the heating tank of the present invention;

FIG. 4 is a front cross-section of a partial structure of the present invention;

FIG. 5 is an enlarged view of a portion of the structure of FIG. 4;

FIG. 6 is a schematic flow chart of the method of the present invention.

In the figure: 1. a base; 2. a heating tank; 3. a peristaltic pump; 4. a condensation growth tank; 5. an air pump; 6. entering a pipe; 7. heating a thermocouple; 8. a steam delivery pipe; 9. a valve; 10. a gas delivery pipe; 11. a collection port; 12. a vertical tube; 13. a collection box; 14. a transverse plate; 15. small holes; 16. an impact plate; 17. a carbon film copper net; 18. a copper pipe; 19. a water inlet pipe; 20. a water outlet pipe; 21. a water delivery control mechanism; 211. a water pump; 212. a first solenoid valve; 213. a second solenoid valve; 22. a support mechanism; 221. a fixing ring; 222. a support bar; 223. a support plate; 23. an exhaust passage; 24. a fan; 25. a fixing member; 26. a box door; 27. and (4) air holes.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, a device for collecting microorganisms in air comprises a base 1, a heating tank 2, a peristaltic pump 3, a condensation growth tank 4 and an air pump 5, wherein the right side of the peristaltic pump 3 is communicated with an inlet pipe 6, the bottom of the inner cavity of the heating tank 2 is provided with a plurality of heating couples 7, the top of the heating tank 2 is communicated with a steam conveying pipe 8, the surface of the steam conveying pipe 8 is provided with a valve 9, one side of the air pump 5 is communicated with an air conveying pipe 10, the other side of the air pump 5 is communicated with a collection port 11, the other ends of the steam conveying pipe 8 and the air conveying pipe 10 are both communicated with the condensation growth tank 4, the bottom of the condensation growth tank 4 is communicated with a vertical pipe 12, the bottom of the vertical pipe 12 is communicated with a collection tank 13, the front of the collection tank 13 is hinged with a tank door 26, a transverse plate 14 is arranged above the inner cavity of the collection tank 13, the surface of the transverse plate 14 is provided with a small hole 15, an impact plate 16 is arranged inside of the collection tank 13 and below the transverse plate 14, the top of striking plate 16 sets up carbon film copper mesh 17, the condensation increases the surface of pond 4 and is provided with copper pipe 18, the both ends of copper pipe 18 communicate respectively has inlet tube 19 and outlet pipe 20, the surface of inlet tube 19 and outlet pipe 20 is provided with water delivery control mechanism 21, the condensation increases the below on pond 4 surface and is provided with supporting mechanism 22, the device adopts the mode that increases the humidity of air and cool down again to reach the supersaturated state, make the microorganism particle diameter in the air increase, and strike on the collector along with inertia when the aperture, thereby accomplish the high-efficient collection to the microorganism, and the device possesses the simple design, therefore, the carrier wave prepaid electric energy meter is low in cost, high durability and convenient use's advantage, the present collection to the microorganism in the air has been solved, mostly adopt the flat method, traditional flat method only can be gathered and can subside the microorganism, and the problem of inefficiency.

Referring to fig. 1, the water delivery control mechanism 21 includes a water pump 211, a first solenoid valve 212 and a second solenoid valve 213, the water pump 211 and the first solenoid valve 212 are both installed on the surface of the water inlet pipe 19, the second solenoid valve 213 is installed on the surface of the water outlet pipe 20, the water pump 211 can be used for delivering cooling water through the arrangement of the water pump 211, the first solenoid valve 212 can control the opening and closing of the water inlet pipe 19, the second solenoid valve 213 can control the opening and closing of the water outlet pipe 20, and the delivery of the cooling water can be controlled through the cooperation of the first solenoid valve 212, the second solenoid valve 213 and the water inlet pipe 19.

Referring to fig. 1 and 2, the supporting mechanism 22 includes a fixing ring 221, a supporting rod 222 and a supporting plate 223, the fixing ring 221 is bolted below the surface of the condensation growth pool 4, one end of the supporting rod 222 is bolted to the fixing ring 221, the supporting plate 223 is bolted to the other end of the supporting rod 222, the supporting plate 223 is bolted to the base 1, through the arrangement of the fixing ring 221, the supporting rod 222 and the supporting plate 223, the fixing ring 221 can fix the supporting rod 222 on the surface of the condensation growth pool 4, and the supporting plate 223 can fix the supporting rod 222 on the surface of the base 1, and the supporting mechanism can assist and fix the condensation growth pool 4 to increase the stability of the condensation growth pool 4.

Referring to fig. 4 and 5, air holes 27 are formed through both sides of the surface of the impact plate 16, the right side of the collection box 13 is communicated with the exhaust passage 23, a fan 24 is installed inside the exhaust passage 23, air can pass through the air holes 27 and the exhaust passage 23 to be discharged through the arrangement of the air holes 27, the exhaust passage 23 and the fan 24, and the fan 24 can increase the flow rate of the discharged air.

Please refer to fig. 1 and 2, the surface of the condensation growth pool 4 is bolted with a plurality of fixing members 25, the copper pipe 18 is spiral, and the copper pipe 18 is fixed with the fixing members 25, the condensation growth pool 4 is made of stainless steel, and the fixing members 25 are arranged to assist the copper pipe 18 to be fixed, and the copper pipe 18 is designed to be spiral, so that the contact area between the copper pipe 18 and the condensation growth pool 4 can be increased, and the cooling heat exchange effect is improved.

Referring to FIGS. 4 and 5, the aperture of the small holes 15 is 0.02-0.1 μm, the distance between the small holes 15 and the impact plate 16 is 0.2-5mm, the inner diameter of the condensation growth tank 4 is 50-80mm, the length of the condensation growth tank 4 is 300-600mm, and the distance between the small holes 15 and the impact plate 16 is 0.2-5mm, which ensures the distance between the transverse plate 14 and the impact plate 16 for the air to flow in, and also ensures that most of the microorganisms in the air are not deviated from the impact air holes 27 and are directly discharged.

A collection method of a device for collecting microorganisms in air comprises the following steps:

step A: the inlet pipe 6 is externally connected with a pure water source, ultrapure water is introduced into the heating pool 2 at the flow rate of 0.5-2ml/min by using the peristaltic pump 3, the heating couple 7 is started to heat the ultrapure water and heat the ultrapure water to 100 ℃ to generate steam, and the valve 9 is started to enable the steam to enter the interior of the condensation growth pool 4 through the steam delivery pipe 8;

and B: starting the air pump 5, so that the outside air and microorganisms enter the condensation growth tank 4 through the acquisition port 11, the air pump 5 and the air delivery pipe 10, and at the moment, the air entering the condensation growth tank 4 is contacted with the water vapor, so that the humidity and the temperature of the air are increased;

and C: the water pump 211 and the first electromagnetic valve 212 are started, cooling water with lower temperature is put into the water inlet pipe 19, at the moment, the cooling water enters the copper pipe 18 through the water inlet pipe 19, then the heat in the air is transferred to the copper pipe 18 through the condensation growth tank 4, the cooling water in the copper pipe 18 absorbs heat, and the temperature of the air in the condensation growth tank 4 is greatly reduced;

step D: the microorganism reaches the oversaturation state under the condition that the humidity of the air is increased and the temperature is reduced, the particle size of the microorganism in the air is increased, then the microorganism runs into the carbon film copper net 17 on the surface of the impact plate 16 under the action of inertia along with the air flow passing through the small holes 15, so that the collection and the capture of the microorganism are completed, and simultaneously the air is discharged through the air holes 27 and the exhaust channel 23.

In the step A, the conductivity of the ultrapure water is more than or equal to 18 mu omega, the design ensures the water degree, and prevents the inside of the device from being easily scaled due to insufficient water purity, thereby preventing the inside of the device from being blocked.

In step B, the flow rate of the gas introduced by the gas pump 5 is 500-1500ml/min, and the design ensures the flow rate of the air and ensures the inertia in the process of microbial impact.

In the step C, the temperature of the cooling water is 0-15 ℃, and the design ensures the temperature of the cooling water and improves the cooling effect on air.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.