阅读说明：本技术 一种植物组织培养用灭菌装置 (Plant tissue culture uses sterilization apparatus ) 是由 黄远志 林顺长 于 2021-09-10 设计创作，主要内容包括：一种植物组织培养用灭菌装置,包括高压蒸汽灭菌锅和抽真空装置,高压蒸汽灭菌锅包括紫外线灯、密封装置、加压装置、锅体、以及存放装置,紫外线灯位于锅体内,锅体安装有压力表、换气孔,存放装置由存放盘和支架组成,密封装置包括密封盖、梁架、手轮,安全阀、密封圈、基准轴,基准轴上安装有手轮和梁架,安全阀安装在密封盖上,抽真空装置包括真空泵以及泵罩,换气孔有出气口和进气口。针对高压蒸汽灭菌锅因排气不彻底,而导致达不到灭菌温度的问题,完全密封后使用抽真空泵将高压蒸汽灭菌锅内的空气抽尽,再通过进气口排入水蒸气,通过加压装置对水蒸气进行加压使其达到最佳灭菌温度。(The utility model provides a plant is sterilization apparatus for tissue culture, including high-pressure steam sterilization pot and evacuating device, high-pressure steam sterilization pot includes ultraviolet lamp, sealing device, pressure device, the pot body, and strorage device, ultraviolet lamp is located the pot internally, the manometer is installed to the pot body, the scavenge port, strorage device comprises storage tray and support, sealing device includes sealed lid, the roof beam structure, the hand wheel, the relief valve, the sealing washer, the reference shaft, install hand wheel and roof beam structure on the reference shaft, the relief valve is installed on sealed covering, evacuating device includes vacuum pump and pump casing, the scavenge port has gas outlet and air inlet. Aiming at the problem that the sterilization temperature can not be reached due to incomplete exhaust of the high-pressure steam sterilization pot, after the high-pressure steam sterilization pot is completely sealed, a vacuum pump is used for completely pumping air in the high-pressure steam sterilization pot, then water vapor is exhausted through an air inlet, and the water vapor is pressurized through a pressurizing device to reach the optimal sterilization temperature.)

1. A plant tissue culture uses sterilization apparatus which characterized in that: including high-pressure steam sterilization pot (1) and evacuating device (2), high-pressure steam sterilization pot (1) includes ultraviolet lamp (11), sealing device (12), pressure device (13), the pot body (14) and strorage device (15), ultraviolet lamp (11) are located in pot body (14), manometer (141), scavenge port (142) are installed to pot body (14), strorage device (15) comprise storage dish (151) and support (152), sealing device (12) are including sealed lid (121), roof beam structure (122), hand wheel (123), relief valve (124), sealing washer (125), reference shaft (126), install on reference shaft (126) hand wheel (123) with roof beam structure (122), relief valve (124) are installed on sealed lid (121), evacuating device (2) include vacuum pump (21) and pump housing (22), the transfer port (142) includes an outlet port (1421) and an inlet port (1422).

2. The sterilization apparatus for plant tissue culture according to claim 1, wherein: the number of the ultraviolet lamps (11) is multiple, and the ultraviolet lamps (11) are arranged on the sealing cover (121) or the pot body (14).

3. The sterilization apparatus for plant tissue culture according to claim 1, wherein: the pot body (14) is provided with a slide rail (143), and the slide rail (143) is provided with a clamping groove (144).

4. A sterilization apparatus for plant tissue culture according to claim 3, wherein: the sliding rail (143) is fixed on the pot body (14) in a welding mode.

5. The sterilization apparatus for plant tissue culture according to claim 1, wherein: the reference shaft (126) is provided with threads, the sealing cover (121) and the beam frame (122) are connected with the reference shaft (126) through threads at separate sides, and the hand wheel (123) is fixed on the reference shaft (126).

6. The sterilization apparatus for plant tissue culture according to claim 5, wherein: and the hand wheel (123) is provided with anti-skid rubber.

7. The sterilization apparatus for plant tissue culture according to claim 1, wherein: the air inlet (1422) is connected with a steam exhaust inlet, and the air outlet (1421) is connected with the vacuumizing device (2).

8. The sterilization apparatus for plant tissue culture according to claim 7, wherein: and the air outlet (1421) is provided with a filter screen (1423).

Technical Field

The invention relates to the technical field of sterilization devices, in particular to a sterilization device for plant tissue culture.

Background

A portable and horizontal high-pressure steam sterilizer is the most common sterilizer used in tissue culture. The temperature of the steam rises along with the increase of the pressure, so that the high-temperature steam for sterilization is obtained by pressurization, the coefficient of thermal expansion of the air is larger than that of the steam, and if the air in the sterilization pot is not eliminated or is not completely eliminated, even if the pressure gauge of the sterilization pot reflects the pressure unit in the pot, the temperature required for sterilization cannot be reached in the sterilization pot under the existence of the air.

Experiments show that if only half of the air in the pot is removed, the pointer of the pressure gauge reaches 0.105 MPa. The actual temperature in the pan can only reach 112 ℃ which is 9 ℃ lower than the required 121 ℃, resulting in incomplete sterilization and possibly causing a large amount of contamination of the culture medium. Therefore, the key to the use of autoclaves is trying to remove the air from the autoclave.

Disclosure of Invention

The invention provides a sterilization device for plant tissue culture, and aims to solve the problem of incomplete sterilization of plant tissue culture.

The utility model provides a plant is sterilization apparatus for tissue culture, includes high-pressure steam sterilization pot and evacuating device, high-pressure steam sterilization pot includes ultraviolet lamp, sealing device, pressure device, the pot body and strorage device, ultraviolet lamp is located the pot is internal, manometer, scavenge port are installed to the pot body, strorage device comprises storage tray and support, sealing device is including sealed lid, roof beam structure, hand wheel, relief valve, sealing washer, reference shaft, install on the reference shaft the hand wheel with the roof beam structure, the relief valve is installed sealed covering, evacuating device includes vacuum pump and pump casing, the scavenge port includes gas outlet and air inlet.

Preferably, the number of the ultraviolet lamps is multiple, and the ultraviolet lamps are arranged on the sealing cover or the pot body.

Preferably, the pot body is provided with a slide rail, and the slide rail is provided with a clamping groove.

Preferably, the sliding rail is fixed on the pot body in a welding mode.

Preferably, the reference shaft is provided with threads, the sealing cover and the beam frame are connected with the reference shaft through the threads, and the hand wheel is fixed on the reference shaft.

Preferably, the hand wheel is provided with anti-skid rubber.

Preferably, the steam inlet of the air inlet is connected with the steam outlet, and the air outlet is connected with the vacuumizing device.

Preferably, the air outlet is provided with a filter screen.

The beneficial technical effects of the invention are as follows:

because this device is using ultraviolet lamp 11 to carry out preliminary sterilization to the device of required sterilization, make bacterium quantity obtain descending for convenient follow-up processing, the unsatisfactory main reason of 1 bactericidal effect of traditional high pressure steam sterilization pot is because the air discharge is incomplete, and leads to reaching required sterilization temperature in the pot body 14, and this high pressure steam sterilization device makes air discharge more thorough through vacuum pump 21 with the air discharge.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the construction of the autoclave of the present invention;

FIG. 3 is a schematic view of the construction of the vacuum apparatus of the present invention;

FIG. 4 is a schematic illustration of the present invention in storage;

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

A sterilization device for plant tissue culture comprises a high-pressure steam sterilization pot 1 and a vacuumizing device 2, wherein the high-pressure steam sterilization pot 1 comprises an ultraviolet lamp 11, a sealing device 12, a pressurizing device 13, a pot body 14 and a storage device 15, the ultraviolet lamp 11 is positioned in the pot body 14, the pot body 14 is provided with a pressure gauge 141 and a ventilating hole 142, the storage device 15 consists of a storage disc 151 and a support 152, the sealing device 12 comprises a sealing cover 121, a beam frame 122 and a hand wheel 123, a safety valve 124, a sealing ring 125 and a reference shaft 126, the reference shaft 126 is provided with the hand wheel 123 and the beam frame 122, the safety valve 124 is arranged on the sealing cover 121, the vacuumizing device 2 comprises a vacuum pump 21 and a pump cover 22, and the ventilating hole 142 is provided with an air outlet 1421 and an air inlet 1422.

Aiming at the problem that the sterilization temperature can not be reached due to incomplete exhaust of the high-pressure steam sterilization pot 1, equipment to be sterilized is placed in the storage device 15, the storage device 15 is placed in the pot cavity through the slide rail 143, the ultraviolet lamp 11 is turned on, the sealing device 12 is used for sealing the equipment, the equipment is further sealed through the hand wheel 123, after the equipment is completely sealed, the vacuum pump is used for completely pumping air in the high-pressure steam sterilization pot 1, water vapor is discharged through the air inlet 1422, and the water vapor is pressurized through the pressurizing device 13 to reach the optimal sterilization temperature.

The number of the ultraviolet lamps 11 is plural, and the ultraviolet lamps 11 are installed on the sealing cover 121 and the pot body 14. Install ultraviolet lamp 11 in each direction, can carry out more effective the disinfecting to the equipment that needs disinfect, carry out first disinfection to equipment, make the effective decline of bacterium quantity make the more thorough of disinfecting. The pot body 14 is provided with a slide rail 143, and the slide rail 143 is provided with a clamping groove 144.

After the sterilization process of the autoclave 1 is completed, the storage device 15 may be lifted by the slide rails 143, and when the storage device 15 is raised to a certain height, the storage device 15 may be fixed to the certain height by the locking grooves 144.

The slide rails 143 are fixed to the pot body 14 by welding. The stability of the system is improved.

The reference shaft 126 is threaded, the seal cap 121 and the beam frame 122 are connected to the reference shaft 126 through threads at their respective sides, and the hand wheel 123 is fixed to the reference shaft 126. The sealing cover 121 is continuously moved downwards by rotating the hand wheel 123, and the whole autoclave 1 is sealed when the sealing cover 121 is contacted with the pot body 14, so that the sealing performance is enhanced.

The hand wheel 123 is provided with rubber with anti-skid lines. The sealing cover 121 is convenient for workers to open and close, and the anti-skidding effect is achieved.

The air inlet 1422 is connected to a steam discharge inlet, and the air outlet 1421 is connected to the vacuum extractor 2. Since the water vapor has a lower density than air, it is discharged from the upper air inlet 1422 when the water vapor is discharged, which further facilitates the discharge of air.

The air outlet 1421 is provided with a filter screen 1423. Preventing foreign matter in the air from being sucked in to damage the vacuum pump 21.

The working principle of the device is as follows:

put into strorage device 15 with the device of required sterilization, strorage device 15 sends required sterilization apparatus into inside the pot body 14 through slide rail 143, the hand wheel 123 that will seal lid 121 and the inseparable bonding of pot body 14 together through being manual, close air inlet 1422, gas outlet 1421 links to each other with evacuating device 2, open the air extraction of vacuum pump 21 in with the pot body 14 clean, in-process ultraviolet lamp 11 at whole extraction is in the open mode, carry out preliminary sterilization to the device of required sterilization, open air inlet 1422 through closing gas outlet 1421 after preliminary sterilization and arrange into pot body 14 with vapor, sterilize through pressurizing pot body 14.

The beneficial technical effects of the invention are as follows:

this device is using ultraviolet lamp 11 to carry out preliminary sterilization to the device of required sterilization, makes bacterium quantity obtain descending for convenient follow-up processing, and 1 sterilizing effect of traditional high pressure steam sterilization pot is unsatisfactory mainly because the air discharge is not thorough, and leads to reaching required sterilizing temperature in the pot body 14, and this high pressure steam sterilization device makes air discharge more thorough through vacuum pump 21 with the air discharge.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.