阅读说明：本技术 一种菊花插穗常温保鲜剂及其应用 (Normal-temperature preservative for chrysanthemum cutting and application thereof ) 是由 罗红辉 伍青 夏涵涵 谢浩然 林政伟 邱民得 李凌 王凤兰 周厚高 于 2021-08-25 设计创作，主要内容包括：本发明公开了一种菊花插穗常温保鲜剂,该保鲜剂包含如下组分：蔗糖21-40g/L,植酸0.7～2mg/L。本发明还公开了该保鲜剂作为菊花插穗常温保鲜剂的应用。蔗糖和植酸两种成分,既能有效保鲜又能促进菊花插穗在经储藏后扦插的生根能力,大大提高了耐储性,保证了成活率,在保证品质前提下,有效降低种苗储运过程损耗率及降低冷链物流成本,产生更高经济效益。(The invention discloses a normal-temperature preservative for chrysanthemum cutting slips, which comprises the following components: 21-40g/L of sucrose and 0.7-2 mg/L of phytic acid. The invention also discloses an application of the preservative as a normal-temperature preservative for chrysanthemum cutting slips. The two components of the sucrose and the phytic acid can effectively keep freshness and promote the rooting capacity of the chrysanthemum cutting after storage, greatly improve the storage resistance, ensure the survival rate, effectively reduce the loss rate of the seedling storage and transportation process and the cost of cold-chain logistics on the premise of ensuring the quality, and generate higher economic benefit.)

1. The normal-temperature preservative for the chrysanthemum cutting slips is characterized by comprising the following components in percentage by concentration: 21-40g/L of sucrose and 0.7-2 mg/L of phytic acid.

2. The chrysanthemum cutting normal-temperature preservative of claim 1, which is characterized by comprising the following components in concentration: 25-35 g/L of sucrose and 1.0-1.5 mg/L of phytic acid.

3. The chrysanthemum cutting normal-temperature preservative of claim 1, which is characterized by comprising the following components in concentration: 30g/L of sucrose and 1.5mg/L of phytic acid.

4. Use of the chrysanthemum cutting normal-temperature preservative according to any one of claims 1 to 3 as a preservative for chrysanthemum cutting.

5. The use according to claim 4, which comprises the step of soaking the chrysanthemum cutting in the chrysanthemum cutting normal-temperature preservative according to claim 1.

6. The use of claim 5, wherein the picking height of the chrysanthemum cutting is 6-8cm, the leaves are 5-7, and the growth vigor is uniform.

7. Use according to claim 5, characterized in that the method is: and soaking the chrysanthemum cutting slips in the chrysanthemum cutting slip normal-temperature preservative for 40-60 minutes, taking out and airing, and storing after simple packaging.

8. Use according to claim 7, wherein the storage conditions are protection from light, a temperature of 20 to 30 ℃ and a humidity of 80% to 93%.

9. Use according to claim 8, wherein the storage conditions are protection from light, a temperature of 25 ℃ and a humidity of 90%.

Technical Field

The invention belongs to the field of chrysanthemum production and cultivation, and particularly relates to a new application of phytic acid in promoting cuttage and rooting of chrysanthemum cuttings after normal-temperature storage.

Background

Chrysanthemum morifolium (Chrysanthemum morifolium) is one of four fresh cut flowers in the world, and is mainly used for appreciation, eating, tea and medicine, and sold in the top of the world. The chrysanthemum is perennial root herbaceous plant of chrysanthemum of Compositae, is native to China, and is introduced into Japan, Europe, North America and other countries. The production of chrysanthemum seedlings is one of the important links in the industrial chain, and the quality of the seedlings directly influences the industrial scale and the economic benefit of chrysanthemum. Cuttage is the most important seedling raising mode in chrysanthemum specialized production, however, in the actual production process, a large number of neat and consistent cutting slips are difficult to obtain, and how to keep the freshness of the cutting slips with proper age in the early stage so as to obtain a large number of neat and consistent cutting slips, so that cuttage (Wangmei and the like, 2015) is arranged according to the planting period, and the method is also an important part for chrysanthemum production. At present, chrysanthemum seedling producers in China mainly produce cutting seedlings, and after the cutting seedlings are harvested, the cutting seedlings need to be tidied, packaged and precooled, and then a cold chain is carried and sent to customers (Xue Jian Ping et al, 2013). With the vigorous development of the chrysanthemum industry, many cuttings and seedlings need to undergo a long and high-temperature storage and transportation process from production to actual transportation to a planting place, the loss rate of the chrysanthemum cuttings and seedlings is high in the long-distance transportation process under a high-temperature environment, and the rooting capacity of the chrysanthemum cuttings and seedlings can be damaged to different degrees after the chrysanthemum cuttings and seedlings are transported for a long distance.

At present, the research on the picked chrysanthemum cutting seedlings at home and abroad is very little, the chrysanthemum seedlings are picked from terminal buds of mature plants, if no fresh-keeping measures are taken, the seedlings continuously transpire and lose water, obvious weight loss is caused in the storage stage, the longer the storage time is, more nutrient substances are consumed in the body under the respiration action, and the permeability of cell membranes is slowly reduced. In practical production, the cutting pretreatment is mostly carried out by soaking in an aqueous solution of carbendazim wettable powder and storing in an environment of 4 ℃. When the chrysanthemum seedlings are stored in an environment of 4 ℃, the storage time for the quality of the seedlings can be ensured to be 3 weeks. Weight loss rapidly increased and survival rate rapidly decreased after 3 weeks of storage (royal plum et al, 2015). The application of a proper amount of nitrogen can obviously prolong the refrigeration time of the chrysanthemum seed seedlings and improve the quality of the seedlings (Liuxin spring, 2013). Although the above measures have certain fresh-keeping and quality-guaranteeing effects, the cost for cold chain storage and transportation is increased greatly. The fresh flower preservative in the prior art generally has complex components, more than ten chemical reagents and more than twenty chemical reagents, is not only complicated to operate, but also can cause certain damage to the activity of fresh flowers. Moreover, the temperature for actual storage and transportation of the chrysanthemum cutting seedlings in China at present can reach over 30 ℃ generally in normal temperature or even high temperature areas, and wilting and yellow leaves are easy to appear, so that the subsequent cutting rooting and planting flower quality and commodity value of the chrysanthemum cutting seedlings are influenced.

Therefore, the preservative which has simple components, can effectively preserve the picked chrysanthemum cutting slips in normal-temperature storage and transportation, can ensure that the cuttage rooting capacity after storage and transportation is the same as that before storage and transportation is developed, and has very high practical value and economic value.

Phytic acid (inositol hexaphosphate, dihydrogen phosphate) is a phytic acid ester of vitamin B group, is strongly acidic and has strong chelating ability, and is a natural substance extracted from grain seed processing by-products. The product has low production cost, rich raw material resources, safety, nontoxicity and no pollution, can be used as food additives such as antioxidant, fermentation aid and the like, and has good fresh-keeping effect, so that the product is widely used in food industry and has extremely wide application prospect (Wangjian et al, 2015).

Disclosure of Invention

The invention aims to provide a formula of a preservative for effectively preserving chrysanthemum cutting slips at normal temperature, so that the storage resistance of the chrysanthemum cutting slips is improved, and the high survival rate of the chrysanthemum cutting slips can be guaranteed after long-time storage and transportation.

The invention aims to solve the technical problems and provides a normal-temperature preservative for chrysanthemum cutting slips.

The invention also provides an application of the chrysanthemum cutting normal-temperature preservative.

In order to realize the aim of the invention, the invention provides a chrysanthemum cutting normal-temperature preservative which comprises the following components in concentration: 21-40g/L of sucrose and 0.7-2 mg/L of phytic acid.

Preferably, the preservative comprises the following components in concentration content: 25-35 g/L of sucrose and 1.0-1.5 mg/L of phytic acid.

Preferably, the preservative comprises the following components in concentration content: 30g/L of sucrose and 1.5mg/L of phytic acid.

The invention also provides the application of the normal-temperature preservative for the chrysanthemum cutting as the preservation of the chrysanthemum cutting.

Further comprising the step of soaking the chrysanthemum cutting in the chrysanthemum cutting normal temperature preservative of claim 1.

Preferably, the picking height of the chrysanthemum cutting is 6-8cm, 5-7 leaves are adopted, and the growth vigor is uniform and consistent.

Further, the method comprises the following steps: and soaking the chrysanthemum cutting slips in the chrysanthemum cutting slip normal-temperature preservative, taking out the chrysanthemum cutting slips after 40-60 minutes, airing, simply packaging and storing.

Preferably, the storage condition is light-proof, the temperature is 20-30 ℃, and the humidity is 80-93%.

Preferably, the storage condition is dark, the temperature is 25 ℃, and the humidity is 90%

Compared with the prior art, the invention has the beneficial effects that:

the chemical preservative for the chrysanthemum cutting slips, provided by the invention, has a simple formula, only contains two components of sucrose and phytic acid, can effectively preserve freshness and promote the rooting capacity of the chrysanthemum cutting slips after storage, greatly improves the storage resistance and ensures the survival rate; the fresh-keeping treatment method is simple to operate, effective fresh keeping can be realized at the normal temperature of 20-24 ℃ or at the high temperature of 25-30 ℃ only by short-time soaking, simple packaging and 80% -93% humidity environment, the loss rate of the seedling storage and transportation process is effectively reduced and the cold-chain logistics cost is reduced on the premise of ensuring the quality, and higher economic benefit is generated.

In the actual production process, the early-stage proper-age cutting shoots are preserved to obtain a large number of neat and consistent cutting shoots, and the rooting time of the cutting shoots is promoted and regulated by phytic acid, so that a large number of neat and consistent chrysanthemum seedlings are obtained, and the industrial scale and the economic benefit of chrysanthemum are greatly improved.

Drawings

FIG. 1 is a comparison graph of the appearance of the fresh-keeping effect of the chrysanthemum cutting of each treatment group;

FIG. 2 is a comparison of rooting of chrysanthemum cuttings after 8 days of normal temperature storage;

Detailed Description

Example 1

The chemical antistaling agent formula of the comparative test of the embodiment is shown in the table 1. CK is a control group, namely most chrysanthemum cuttings are not subjected to any fresh-keeping treatment before storage and transportation at present; group A AgNO₃The aqueous solution is one of the most commonly used components of the cut flower preservative; the water solution of the sucrose in the group B provides basic nutrients required by the cutting slips during storage and transportation; the group C and the group D are both test groups containing phytic acid; wherein the phytic acid solid reagent contains 90% of active ingredient (CAS:83-86-3), is available from Shanghai Michelin Biotechnology Ltd (MACKLIN), and is easily soluble in water.

1. Preservative formula solution

Table 1 formula of antistaling agent used in comparative experiment

2. Preservation treatment after cutting and picking

Picking up the cutting of the chrysanthemum in 'Hongri', the height of the cutting is 6-8cm, the number of leaves is 5-7, and the growth vigor is uniform; and (3) respectively putting the cuttings into various preservative solutions described in the table 1, soaking for 45min, then putting the cuttings on absorbent paper, airing the cuttings in the shade until no moisture remains on the surfaces of the cuttings.

3. Storage of

The treated cutting is placed in an intelligent artificial climate box with 25 ℃, 90% humidity and dark environment for normal temperature storage for 8 days, and corresponding physiological and biochemical indexes are observed and detected by taking pictures periodically in the period.

4. The evaluation of the chrysanthemum cutting after the soaking treatment showed that the chrysanthemum cutting of test group C was still good in appearance quality after being stored for 8 days, and the chrysanthemum cutting of test group D was relatively good in appearance quality, as shown in fig. 1. Yellow leaf rate statistics is carried out on each group of chrysanthemum cutting slips after soaking treatment, cutting slip weight loss rate, relative conductivity of cutting slip leaves and chlorophyll SPAD value are detected, and statistics and detection results are shown in table 2.

a. Percent yellow leaf ratio (%) (number of cuttings with yellow leaves × 100)/total number of cuttings

b. Weight loss rate of cutting

Taking 3 groups (namely 3 replicates) of each chrysanthemum cutting sample, weighing 30 cutting in each group, and weighing the fresh weight m of each group of cutting before storage₀And fresh weight of each storage day, and the average of 3 replicates was taken as the fresh weight m_x. By the formula: weight loss ratio (%) ═ m₀-m_x)×100/m₀Calculating the weight loss ratio

c. Relative conductivity of cutting blade

Taking the chrysanthemum cutting leaves with consistent development, quantitatively taking 3 parts (namely 3 repetitions), cutting each part into pieces of 2g, respectively placing the pieces into 3 triangular flasks, adding 20mL of deionized water into each triangular flask, and completely soaking the materials in the water. Placing the triangular flask on a shaking table, oscillating for 3h (40-60 times/min), measuring the conductivity with a conductivity meter, and measuring the conductivity with a conductivity meter₁Represents; then, the flask was placed in a boiling water bath to boil for 15min, and after cooling to room temperature, the total conductivity was measured using R₂And (4) showing. Relative leaf conductivity (%) ═ R₁×100)/R₂。

d. Chlorophyll SPAD value

On day 8 of chrysanthemum cutting storage, the central leaves (i.e. 10 replicates) of 10 cutting plants in each group were taken, the chlorophyll SPAD value of each leaf was measured using a SPAD-502Plus chlorophyll meter (Konica Minolta, Japan), and the relative chlorophyll content was obtained by averaging.

TABLE 2 determination of the physiological and biochemical indicators after 8 days of storage at Normal temperature (25 deg.C, humidity 90%)

Treatment group	Yellow leaf rate	Weight loss ratio	Relative electrical conductivity of the blades	Chlorophyll SPAD value
					CK	90.6％	29.1％	50.3％	42.7
A	70.7％	24.8％	51.5％	46.3
					B	68.8％	20.4％	49.7％	48.5
C	58.3％	10.3％	33.1％	49.7
					D	63.9％	15.3％	38.2％	45.2

And (4) analyzing results: as can be seen from the data in Table 2, after 8 days of storage at normal temperature, the yellow leaf rate of the cutting shoot in the test group C and the yellow leaf rate of the cutting shoot in the test group D containing phytic acid are respectively 58.3 percent and 63.9 percent in the treatment of 5 preservatives, and both are obviously lower than the yellow leaf rate of 90.6 percent in the control CK group. Indexes of weight loss rate, relative leaf conductivity and chlorophyll SPAD value of C are obviously superior to those of other treatments, which shows that the formula concentration of the preservative C is more suitable for normal-temperature preservation of chrysanthemum cuttings in red days. In addition, the appearance of the cutting is as shown in figure 1.

5. Statistics of cutting survival rate

The chrysanthemum cutting slips of each treatment group are respectively cut in a plug tray after being stored for 8 days, meanwhile, the fresh chrysanthemum cutting slips which are not stored are used as a comparison group, namely a CK group in a table 3, the plug tray uses wet river sand as a substrate, is uniformly placed in a growth environment of 25 ℃ for 16h day/8 h night, is sprayed with a 'Jinlei' brand bactericide (component: refined methanol cream manganese zinc) every 2 days, the cutting slips are pulled out after the chrysanthemum cutting slips grow for 15 days, the rooting condition is counted, and the chrysanthemum cutting slips are judged to be alive if the root system is obviously visible by naked eyes. The results in table 3 show that the cutting survival rate of the cuttings treated and stored by the preferred phytic acid antistaling agent reaches 100%.

TABLE 3 statistics of rooting of cuttings after storage at Normal temperature (15 days after cutting)

Treatment group

Survival rate

Time to root

Root number of single plant

Total fresh weight of root system of single plant

Average root length of individual plant

Longest root length of single plant

CK

99.8％

Day 6

20

0.0387g

5.05cm

6.97cm

A

88％

Day 10

10

0.0115g

1.30cm

3.6cm

B

100％

Day 7

22

0.0452g

5.02cm

7.27cm

C

100％

Day 5

23

0.0480g

5.09cm

7.92cm

D

100％

Day 5.5

22

0.0385g

5.07cm

7.75cm

6. Statistics of rooting conditions

And measuring and recording the rooting time, the rooting number, the total fresh weight of the root system, the average root length and the longest root length of each single cutting, and respectively measuring and recording 6 cuttings each time and averaging. The results in Table 3 show that the rooting capacity of the chrysanthemum cuttings in the groups C and D after storage and transportation at normal temperature is the same as that of fresh chrysanthemum cuttings, and the chrysanthemum cuttings can root even faster and have better rooting effect.

After the cutting slips of the treatment group C are stored for 8 days at normal temperature, the rooting number of the single plant, the total fresh weight of the root system of the single plant, the average root length of the single plant and the longest root length of the single plant after cutting rooting culture for 15 days are all obviously higher than those of the other treatment groups, the effect of the treatment group D is inferior, the rooting condition of the treatment group A is worst, and the rooting condition of Ag is shown to be the worst⁺Probably inhibits the rooting of the chrysanthemum cutting. In addition, the rooting of the cuttings of each treatment group is compared with that of FIG. 2.

Example 2

1. Preservation treatment after cutting and picking

Picking up chrysanthemum cuttings with the height of 6-8cm and 5-7 leaves, and enabling the chrysanthemum cuttings to grow uniformly; and (3) putting the cutting into the preservative solution containing 21g/L of sucrose and 0.7mg/L of phytic acid, soaking for 40min, then putting on absorbent paper, airing in the shade until no moisture remains on the surface of the cutting.

3. Storage of

The chrysanthemum cutting slips soaked by the preservative solution are simply packaged by a porous plastic bag or a perforated self-sealing bag and then are stored in an intelligent artificial climate box with the temperature of 20 ℃ and the humidity of 90 percent in a dark environment.

4. The chrysanthemum cutting after the soaking treatment is evaluated, and the result shows that the appearance quality of the chrysanthemum cutting stored for 8 days is still good. Yellow leaf rate statistics is carried out on the chrysanthemum cutting used in the test, and cutting weight loss rate, relative conductivity of cutting leaves and chlorophyll SPAD value are detected.

a. The yellow leaf ratio (%) (number of cuttings with yellow leaves × 100)/total number of cuttings, 57.2%.

b. Weight loss rate of cutting

Taking 3 groups of chrysanthemum cutting samples, weighing 30 cutting samples in each group, and weighing the fresh weight m of the chrysanthemum cutting before storage₀And fresh weight m of day 8 in storage_x. Take 3 groups of mean values, by the formula: weight loss ratio (%) ═ m₀-m_x)×100/m₀And calculating to obtain the weight loss rate of 12.0%. The weight loss rate of the chrysanthemum cutting slips which are not soaked in the preservative solution is 25.3 percent.

c. Relative conductivity of cutting blade

And (3) storing the chrysanthemum cutting on day 8, taking 2g of leaves of the chrysanthemum cutting with consistent development, cutting the leaves into pieces, placing the pieces into a triangular flask, adding 20mL of deionized water, completely soaking the materials in the water, placing the triangular flask on a shaking table, oscillating the materials for 3 hours (40-60 times/min), measuring the conductivity by using a conductivity meter, and using R to measure the conductivity of the materials, wherein R is used for measuring the conductivity of the materials₁Represents; then, the flask was placed in a boiling water bath to boil for 15min, and after cooling to room temperature, the total conductivity was measured using R₂And (4) showing. Relative leaf conductivity (%) ═ R₁×100)/R₂The relative conductivity was calculated to be 32.6%. The conductivity of the chrysanthemum cutting which is not soaked in the preservative solution is 48.8 percent.

d. Chlorophyll SPAD value

On the 8 th day of the storage of the chrysanthemum cutting, 10 middle leaves of the cutting plant are taken, the chlorophyll SPAD value of each leaf is measured by using a SPAD-502Plus chlorophyll meter (Konica Minolta, Japan), the average value is taken to be 49.4, and the chlorophyll SPAD value of the chrysanthemum cutting which is not soaked in the preservative solution is 45.7.

5. Statistics of cutting survival rate

The chrysanthemum cutting shoots are stored for 8 days and then are cut in a plug tray, the plug tray uses wet river sand as a substrate, is uniformly placed in a growth environment with the temperature of 25 ℃ and the day time of 16 h/night of 8h, and is sprayed with a 'Jinlei' brand bactericide (component: refined methanol, manganese and zinc). Randomly selecting 3 cuttings every 12 hours from the 3 rd day to observe whether the cuttings have rooted, and judging the cuttings to be rooted if the cuttings are visible by naked eyes; after 15 days of growth, all the cuttings are pulled out, the rooting conditions are counted, and the cuttings are judged to survive if the root system is obviously visible to naked eyes.

In the embodiment, visible roots can grow on the 5.5 th day after the chrysanthemum cutting shoot is subjected to cuttage, and the survival rate is 100%.

Example 3

1. Preservation treatment after cutting and picking

Picking up chrysanthemum cuttings with the height of 6-8cm and 5-7 leaves, and enabling the chrysanthemum cuttings to grow uniformly; and (3) putting the cutting into the preservative solution containing 40g/L of cane sugar and 1.8mg/L of phytic acid, soaking for 40min, then putting on absorbent paper, airing in the shade until no moisture remains on the surface of the cutting.

3. Storage of

The chrysanthemum cutting slips soaked by the preservative solution are simply packaged by a porous plastic bag or a perforated self-sealing bag and then are stored in an intelligent artificial climate box with the temperature of 25 ℃, the humidity of 80 percent and the dark environment.

4. The chrysanthemum cutting after the soaking treatment is evaluated, and the result shows that the appearance quality of the chrysanthemum cutting stored for 8 days is still good. Yellow leaf rate statistics is carried out on the chrysanthemum cutting used in the test, and cutting weight loss rate, relative conductivity of cutting leaves and chlorophyll SPAD value are detected.

a. The yellow leaf ratio (%) (number of cuttings with yellow leaves × 100)/total number of cuttings, 59.0%.

b. Weight loss rate of cutting

Taking 3 groups of chrysanthemum cutting samples, weighing 30 cutting samples in each group, and weighing the fresh weight m of the chrysanthemum cutting before storage₀And fresh weight m of day 8 in storage_x. Take 3 groups of mean values, by the formula: weight loss ratio (%) ═ m₀-m_x)×100/m₀And calculating to obtain the weight loss rate of 16.4%. The weight loss rate of the chrysanthemum cutting slips which are not soaked in the preservative solution is 31.6 percent.

c. Relative conductivity of cutting blade

And (3) storing the chrysanthemum cutting on day 8, taking 2g of leaves of the chrysanthemum cutting with consistent development, cutting the leaves into pieces, placing the pieces into a triangular flask, adding 20mL of deionized water, completely soaking the materials in the water, placing the triangular flask on a shaking table, oscillating the materials for 3 hours (40-60 times/min), measuring the conductivity by using a conductivity meter, and using R to measure the conductivity of the materials, wherein R is used for measuring the conductivity of the materials₁Represents; then, the flask was placed in a boiling water bath to boil for 15min, and after cooling to room temperature, the total conductivity was measured using R₂And (4) showing. Relative leaf conductivity (%) ═ R₁×100)/R₂The relative conductivity was calculated to be 34%. The conductivity of the chrysanthemum cutting which is not soaked in the preservative solution is 52.3 percent.

d. Chlorophyll SPAD value

On the 8 th day of the storage of the chrysanthemum cutting, 10 middle leaves of the cutting plant are taken, the chlorophyll SPAD value of each leaf is measured by using a SPAD-502Plus chlorophyll meter (Konica Minolta, Japan), the average value is taken to be 48.0, and the chlorophyll SPAD value of the chrysanthemum cutting which is not soaked in the preservative solution is 45.7.

5. Statistics of cutting survival rate

The chrysanthemum cutting shoots are stored for 8 days and then are cut in a plug tray, the plug tray uses wet river sand as a substrate, is uniformly placed in a growth environment with the temperature of 25 ℃ and the day time of 16 h/night of 8h, and is sprayed with a 'Jinlei' brand bactericide (component: refined methanol, manganese and zinc). Randomly selecting 3 cuttings every 12 hours from the 3 rd day to observe whether the cuttings have rooted, and judging the cuttings to be rooted if the cuttings are visible by naked eyes; after 15 days of growth, all the cuttings are pulled out, the rooting conditions are counted, and the cuttings are judged to survive if the root system is obviously visible to naked eyes.

The visible roots can grow on the 5 th day after the chrysanthemum cutting is subjected to cuttage, and the survival rate is 100%.

Example 4

1. Preservation treatment after cutting and picking

Picking up chrysanthemum cuttings with the height of 6-8cm and 5-7 leaves, and enabling the chrysanthemum cuttings to grow uniformly; and (3) putting the cutting into the preservative solution containing 25g/L of cane sugar and 2.0mg/L of phytic acid, soaking for 50min, then putting on absorbent paper, airing in the shade until no moisture remains on the surface of the cutting.

3. Storage of

The chrysanthemum cutting slips soaked by the preservative solution are simply packaged by a porous plastic bag or a perforated self-sealing bag and then are stored in an intelligent artificial climate box with the temperature of 30 ℃ and the humidity of 93 percent in a dark environment.

4. The chrysanthemum cutting after the soaking treatment is evaluated, and the result shows that the appearance quality of the chrysanthemum cutting stored for 8 days is still good. Yellow leaf rate statistics is carried out on the chrysanthemum cutting used in the test, and cutting weight loss rate, relative conductivity of cutting leaves and chlorophyll SPAD value are detected.

a. The yellow leaf ratio (%) (number of cuttings with yellow leaves × 100)/total number of cuttings, 63.0%.

b. Weight loss rate of cutting

Taking 3 groups of chrysanthemum cutting samples, weighing 30 cutting samples in each group, and weighing the fresh weight m of the chrysanthemum cutting before storage₀And fresh weight m of day 8 in storage_x. Take 3 groups of mean values, by the formula: weight loss ratio (%) ═ m₀-m_x)×100/m₀And calculating to obtain the weight loss rate of 17.2%. The weight loss rate of the chrysanthemum cutting slips which are not soaked in the preservative solution is 35.6 percent.

c. Relative conductivity of cutting blade

And (3) storing the chrysanthemum cutting on day 8, taking 2g of leaves of the chrysanthemum cutting with consistent development, cutting the leaves into pieces, placing the pieces into a triangular flask, adding 20mL of deionized water, completely soaking the materials in the water, placing the triangular flask on a shaking table, oscillating the materials for 3 hours (40-60 times/min), measuring the conductivity by using a conductivity meter, and using R to measure the conductivity of the materials, wherein R is used for measuring the conductivity of the materials₁Represents; then, the triangular flask is put into a boiling water bath to be boiled for 15min, and after the triangular flask is cooled to the room temperature, the total electricity is measuredConductivity, by R₂And (4) showing. Relative leaf conductivity (%) ═ R₁×100)/R₂The relative conductivity was calculated to be 36.5%. The conductivity of the chrysanthemum cutting which is not soaked in the preservative solution is 54.5 percent.

d. Chlorophyll SPAD value

On the 8 th day of the storage of the chrysanthemum cutting, 10 middle leaves of the cutting plant are taken, the chlorophyll SPAD value of each leaf is measured by using a SPAD-502Plus chlorophyll meter (Konica Minolta, Japan), the average value is taken to be 48.8 of the chlorophyll SPAD value, and the chlorophyll SPAD value of the chrysanthemum cutting which is not soaked in the preservative solution is 45.2.

5. Statistics of cutting survival rate

The chrysanthemum cutting shoots are stored for 8 days and then are cut in a plug tray, the plug tray uses wet river sand as a substrate, is uniformly placed in a growth environment with the temperature of 25 ℃ and the day time of 16 h/night of 8h, and is sprayed with a 'Jinlei' brand bactericide (component: refined methanol, manganese and zinc). Randomly selecting 3 cuttings every 12 hours from the 3 rd day to observe whether the cuttings have rooted, and judging the cuttings to be rooted if the cuttings are visible by naked eyes; after 15 days of growth, all the cuttings are pulled out, the rooting conditions are counted, and the cuttings are judged to survive if the root system is obviously visible to naked eyes.

In the embodiment, the visible roots can grow on the 4.5 th day after the chrysanthemum cutting shoot is subjected to cuttage, and the survival rate is 100%.

Example 5

1. Preservation treatment after cutting and picking

Picking up chrysanthemum cuttings with the height of 6-8cm and 5-7 leaves, and enabling the chrysanthemum cuttings to grow uniformly; and (3) putting the cutting into the preservative solution containing 30g/L of cane sugar and 1.0mg/L of phytic acid, soaking for 50min, then putting on absorbent paper, airing in the shade until no moisture remains on the surface of the cutting.

3. Storage of

The chrysanthemum cutting slips soaked by the preservative solution are simply packaged by a porous plastic bag or a perforated self-sealing bag and then are stored in an intelligent artificial climate box with the temperature of 25 ℃ and the humidity of 90 percent in a dark environment.

4. The chrysanthemum cutting after the soaking treatment is evaluated, and the result shows that the appearance quality of the chrysanthemum cutting stored for 8 days is still good. Yellow leaf rate statistics is carried out on the chrysanthemum cutting used in the test, and cutting weight loss rate, relative conductivity of cutting leaves and chlorophyll SPAD value are detected.

a. The yellow leaf ratio (%) (number of cuttings with yellow leaves × 100)/total number of cuttings, 58.9%.

b. Weight loss rate of cutting

Taking 3 groups of chrysanthemum cutting samples, weighing 30 cutting samples in each group, and weighing the fresh weight m of the chrysanthemum cutting before storage₀And fresh weight m of day 8 in storage_x. Take 3 groups of mean values, by the formula: weight loss ratio (%) ═ m₀-m_x)×100/m₀And the weight loss rate is calculated to be 14.7%. The weight loss rate of the chrysanthemum cutting slips which are not soaked in the preservative solution is 30.6 percent.

c. Relative conductivity of cutting blade

And (3) storing the chrysanthemum cutting on day 8, taking 2g of leaves of the chrysanthemum cutting with consistent development, cutting the leaves into pieces, placing the pieces into a triangular flask, adding 20mL of deionized water, completely soaking the materials in the water, placing the triangular flask on a shaking table, oscillating the materials for 3 hours (40-60 times/min), measuring the conductivity by using a conductivity meter, and using R to measure the conductivity of the materials, wherein R is used for measuring the conductivity of the materials₁Represents; then, the flask was placed in a boiling water bath to boil for 15min, and after cooling to room temperature, the total conductivity was measured using R₂And (4) showing. Relative leaf conductivity (%) ═ R₁×100)/R₂The relative conductivity was calculated to be 35.7%. The conductivity of the chrysanthemum cutting which is not soaked in the preservative solution is 50.5 percent.

d. Chlorophyll SPAD value

On the 8 th day of the chrysanthemum cutting storage, 10 middle leaves of the cutting plants are taken, the chlorophyll SPAD value of each leaf is measured by using a SPAD-502Plus chlorophyll meter (Konica Minolta, Japan), the average value is taken to be 49.2 of the chlorophyll SPAD value, and the chlorophyll SPAD value of the chrysanthemum cutting which is not soaked in the preservative solution is 42.4.

5. Statistics of cutting survival rate

The chrysanthemum cutting shoots are stored for 8 days and then are cut in a plug tray, the plug tray uses wet river sand as a substrate, is uniformly placed in a growth environment with the temperature of 25 ℃ and the day time of 16 h/night of 8h, and is sprayed with a 'Jinlei' brand bactericide (component: refined methanol, manganese and zinc). Randomly selecting 3 cuttings every 12 hours from the 3 rd day to observe whether the cuttings have rooted, and judging the cuttings to be rooted if the cuttings are visible by naked eyes; after 15 days of growth, all the cuttings are pulled out, the rooting conditions are counted, and the cuttings are judged to survive if the root system is obviously visible to naked eyes.

In the embodiment, visible roots can grow on the 5.5 th day after the chrysanthemum cutting shoot is subjected to cuttage, and the survival rate is 100%.

Example 6

1. Preservation treatment after cutting and picking

Picking up chrysanthemum cuttings with the height of 6-8cm and 5-7 leaves, and enabling the chrysanthemum cuttings to grow uniformly; and (3) putting the cutting into the preservative solution containing 20g/L of cane sugar and 2.0mg/L of phytic acid, soaking for 40min, then putting on absorbent paper, airing in the shade until no moisture remains on the surface of the cutting.

3. Storage of

The chrysanthemum cutting slips soaked by the preservative solution are simply packaged by a porous plastic bag or a perforated self-sealing bag and then are stored in an intelligent artificial climate box with the humidity of 30 ℃ and 88 percent in a dark environment.

4. The chrysanthemum cutting after the soaking treatment is evaluated, and the result shows that the appearance quality of the chrysanthemum cutting stored for 8 days is still good. Yellow leaf rate statistics is carried out on the chrysanthemum cutting used in the test, and cutting weight loss rate, relative conductivity of cutting leaves and chlorophyll SPAD value are detected.

a. Percent yellow leaf ratio (%) (number of cuttings with yellow leaves × 100)/total number of cuttings, 67.0%

b. Weight loss rate of cutting

Taking 3 groups of chrysanthemum cutting samples, weighing 30 cutting samples in each group, and weighing the fresh weight m of the chrysanthemum cutting before storage₀And fresh weight m of day 8 in storage_x. Take 3 groups of mean values, by the formula: weight loss ratio (%) ═ m₀-m_x)×100/m₀And calculating to obtain the weight loss rate of 17.7%. The weight loss rate of the chrysanthemum cutting slips which are not soaked in the preservative solution is 32.8 percent.

c. Relative conductivity of cutting blade

On day 8 of chrysanthemum cutting storage, 2g of chrysanthemum cutting leaves with consistent development are taken, cut into pieces, placed in a triangular flask, and added with 20mL of deionized waterSoaking the materials in water, placing the triangular flask on a shaking table, oscillating for 3 hr (40-60 times/min), measuring conductivity with a conductivity meter, and measuring conductivity with R₁Represents; then, the flask was placed in a boiling water bath to boil for 15min, and after cooling to room temperature, the total conductivity was measured using R₂And (4) showing. Relative leaf conductivity (%) ═ R₁×100)/R₂The relative conductivity was calculated to be 37.2%. The conductivity of the chrysanthemum cutting which is not soaked in the preservative solution is 54.3 percent.

d. Chlorophyll SPAD value

On the 8 th day of the chrysanthemum cutting storage, 10 middle leaves of the cutting plants are taken, the chlorophyll SPAD value of each leaf is measured by using a SPAD-502Plus chlorophyll meter (Konica Minolta, Japan), the average value is taken to be 48.5, and the chlorophyll SPAD value of the chrysanthemum cutting which is not soaked in the preservative solution is 41.0.

5. Statistics of cutting survival rate

The chrysanthemum cutting shoots are stored for 8 days and then are cut in a plug tray, the plug tray uses wet river sand as a substrate, is uniformly placed in a growth environment with the temperature of 25 ℃ and the day time of 16 h/night of 8h, and is sprayed with a 'Jinlei' brand bactericide (component: refined methanol, manganese and zinc). Randomly selecting 3 cuttings every 12 hours from the 3 rd day to observe whether the cuttings have rooted, and judging the cuttings to be rooted if the cuttings are visible by naked eyes; after 15 days of growth, all the cuttings are pulled out, the rooting conditions are counted, and the cuttings are judged to survive if the root system is obviously visible to naked eyes.

In the embodiment, the visible roots can grow on the 4.5 th day after the chrysanthemum cutting shoot is subjected to cuttage, and the survival rate is 100%.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.