阅读说明：本技术 一种适用于生物硬组织材料的超声共振谱测试平台 (Ultrasonic resonance spectrum testing platform suitable for biological hard tissue material ) 是由 樊璠 牛海军 徐连胜 于 2021-08-11 设计创作，主要内容包括：超声共振谱方法因其单次测量可获得样本全部独立的弹性常数,测量不受尺寸限制且测量精度高等优势,近年来被拓展应用于小尺寸生物硬组织材料弹性性质测量中。本发明设计了一种适用于生物硬组织材料的超声共振谱测试平台,主要用于准确检测小尺寸生物硬组织材料的超声共振频率,具体涉及生物医学电子技术领域,包括：第一部分,超声激励与接收模块；第二部分：测试参数设置模块。本发明可实现生物硬组织材料的超声共振频率精确测量,对生物硬组织材料力学性能评价有重要意义。(The ultrasonic resonance spectrum method has the advantages that all independent elastic constants of samples can be obtained through single measurement, the measurement is not limited by size, the measurement precision is high, and the like, and the method is expanded to be applied to the measurement of the elastic properties of small-size biological hard tissue materials in recent years. The invention designs an ultrasonic resonance spectrum testing platform suitable for biological hard tissue materials, which is mainly used for accurately detecting the ultrasonic resonance frequency of small-size biological hard tissue materials, and particularly relates to the technical field of biomedical electronics, and comprises the following steps: a first part, an ultrasonic excitation and receiving module; a second part: and a test parameter setting module. The invention can realize the accurate measurement of the ultrasonic resonance frequency of the biological hard tissue material and has important significance for the evaluation of the mechanical property of the biological hard tissue material.)

1. An ultrasonic resonance spectrum testing platform suitable for biological hard tissue materials, which is characterized by comprising:

the ultrasonic excitation and receiving module is used for exciting the biological hard tissue sample to generate resonance and realizing resonance frequency spectrum receiving;

the test parameter setting module is used for setting ultrasonic resonance spectrum experiment parameters;

the ultrasonic excitation and reception module includes: the ultrasonic probe comprises a pulse generating circuit module, an ultrasonic probe and a signal processing circuit module;

the parameter setting module includes: an excitation parameter setting module and a receiving parameter setting module,

wherein:

the pulse generating circuit module is used for generating a frequency sweep signal with specific frequency range and power;

the ultrasound probe comprises two lightweight shear wave ultrasound probes, namely:

the first ultrasonic probe is electrically connected with the pulse generating circuit module, the surface of the first ultrasonic probe is placed upwards, and the first ultrasonic probe is used for exciting the biological hard tissue to resonate and acting on the biological hard tissue material to be detected;

and the surface of the second ultrasonic probe is downwards placed and is used for receiving the resonance frequency spectrum of the biological hard tissue material sample.

2. The ultrasonic resonance spectroscopy test platform for biological hard tissue materials according to claim 1, wherein:

the biological hard tissue sample comprises but is not limited to a material sample such as human enamel, dentin, cortical bone, cancellous bone and the like, and is diagonally placed between the two ultrasonic probes.

3. The ultrasonic resonance spectroscopy test platform for biological hard tissue materials according to claim 1, wherein:

the signal processing circuit module is electrically connected with the second ultrasonic probe, amplifies the output signal, performs analog-digital conversion, and realizes storage of the resonance frequency spectrum of the biological hard tissue material sample.

4. The ultrasonic resonance spectroscopy test platform for biological hard tissue materials according to claim 1, wherein:

the excitation parameter setting module is used for setting excitation intensity, frequency range and frequency resolution.

5. The ultrasonic resonance spectroscopy test platform for biological hard tissue materials according to claim 1, wherein:

the receiving parameter setting module is used for setting receiving bandwidth.

6. The ultrasonic resonance spectrum testing method based on the ultrasonic resonance spectrum testing platform according to one of claims 1 to 5, characterized by comprising the following steps:

a small-size biological hard tissue sample to be detected is placed between a first ultrasonic probe (102) and a second ultrasonic probe (103),

the excitation parameters including excitation intensity, frequency range and frequency resolution are set by an excitation parameter setting module,

a pulse generating circuit module (101) is used for generating a frequency sweep signal with a specific frequency range,

enabling the sweep frequency signal to excite a small-size biological hard tissue sample (105) to be measured to generate resonance through the first ultrasonic probe (102),

receiving a resonance spectrum output signal of a small-sized biological hard tissue sample (105) to be measured with a second ultrasonic probe (104),

setting parameters including a reception bandwidth by a reception parameter setting module (202) and inputting a resonance spectrum output signal to a signal processing module (104),

and amplifying the resonance spectrum output signal by using a signal processing module (104) and carrying out analog-digital conversion, thereby realizing the resonance spectrum test of the small-size biological hard tissue sample (105) to be tested.

7. The ultrasonic resonance spectroscopy test method according to claim 6, further comprising:

the small-sized biological hard tissue sample (105) to be tested is in a free surface boundary condition state.

Technical Field

The invention relates to an ultrasonic resonance spectrum testing platform suitable for biological hard tissue materials.

Background

The accurate measurement of the mechanical properties of biological hard tissues such as bones, teeth and the like is of great significance for understanding functions, revealing mechanisms and designing, preparing and evaluating implantable prostheses.

Due to the high hardness, large brittleness, small size and difficult sampling of biological hard tissue materials, the existing mechanical testing method has the defects of poor consistency and poor repeatability of testing results, mostly destructive measurement and incapability of clamping small-size samples. Compared with the existing mechanical testing method, the ultrasonic resonance spectrum has the advantages of being capable of realizing nondestructive measurement, not limited by sample size, capable of obtaining all elastic constants through single measurement, highly repeatable in result and the like, and is widely applied to mechanical property testing of metal or crystal materials.

However, the method still has certain limitations in the measurement of the mechanical properties of the biological hard tissue, and one important reason is the lack of an ultrasonic resonance spectrum testing platform suitable for the biological hard tissue. Compared with a crystal material, the biological hard tissue material is not a standard elastomer, so that the biological hard tissue material has stronger viscoelasticity in the ultrasonic resonance measurement process, specifically, the resonance spectrum is flat, and two adjacent resonance peaks are overlapped more. And the attenuation to ultrasonic energy is larger in the measurement process of the biological hard tissue material. In addition, the bio-hard tissue samples are typically smaller in size, corresponding to their higher resonant frequency. Therefore, the ultrasonic resonance spectrum testing system suitable for the biological hard tissue material has higher requirements on testing precision, resolution, excitation source, excitation frequency and other parameters.

Disclosure of Invention

The invention provides an ultrasonic resonance spectrum testing platform suitable for biological hard tissue materials, and can provide more advanced technical means for accurately describing the mechanical properties of biological hard tissues.

Aiming at the requirements of parameters such as testing precision, resolution, excitation source, excitation frequency and the like in the ultrasonic resonance spectrum test of the small-size biological hard tissue sample, the invention aims to provide an ultrasonic resonance spectrum measuring platform suitable for biological hard tissue materials, and the accurate measurement of the resonance frequency of the small-size biological hard tissue sample is realized.

In order to achieve the purpose, the invention adopts the technical scheme that: an ultrasonic resonance spectroscopy measurement platform suitable for biological hard tissue materials, comprising: the ultrasonic excitation and receiving module and the test parameter setting module; the ultrasonic excitation and receiving module is used for exciting the biological hard tissue sample to generate resonance and realizing resonance frequency spectrum receiving; the test parameter setting module is used for setting ultrasonic resonance spectrum experiment parameters;

wherein:

the pulse generating circuit module is used for generating an electric signal (frequency sweep signal) with specific frequency range and power; the ultrasonic probe is a group of (2) light shear wave ultrasonic probes, the first ultrasonic probe is electrically connected with the pulse generation circuit module, and the surface of the probe is placed upwards and used for exciting the biological hard tissue to resonate; acting on the biological hard tissue material to be detected; the second ultrasonic probe is placed with the surface downward and is used for receiving the resonance frequency spectrum of the biological hard tissue material sample. The signal processing circuit module is electrically connected with the second ultrasonic probe, amplifies the output signal, performs analog-digital conversion and realizes storage of the resonance frequency spectrum of the biological hard tissue sample;

the excitation parameter setting module is used for setting parameters such as excitation intensity, frequency range, frequency resolution and the like; the receiving parameter setting module is used for setting parameter settings such as receiving bandwidth and the like.

The beneficial effects of the invention include:

the invention realizes the accurate measurement of the resonance frequency of the biological hard tissue material, provides more advanced technical means for the accurate description of the mechanical characteristics of the biological hard tissue, and lays a foundation for the product design of the biological hard tissue material testing equipment based on the ultrasonic resonance spectrum.

Drawings

FIG. 1 is a schematic diagram of an ultrasonic resonance spectroscopy test platform suitable for use with biological hard tissue materials according to one embodiment of the invention;

FIG. 2 is a graph of ultrasonic resonance spectroscopy measurements suitable for use with biological hard tissue materials according to one embodiment of the invention;

FIG. 3 is a flow chart of the use of ultrasonic resonance spectroscopy for biological hard tissue materials according to one embodiment of the present invention.

Reference numerals:

101-a pulse generating circuit module; 102-a first ultrasound probe; 103-a second ultrasound probe; 104-a signal processing circuit module; 105-a biological hard tissue sample to be tested; 201-excitation parameter setting module; 202-receiving a parameter setting module.

Detailed Description

The invention aims to design an ultrasonic resonance spectrum measuring platform suitable for biological hard tissue materials, which can realize accurate resonance frequency of a biological hard tissue material sample, and specifically comprises the following steps: the ultrasonic excitation and receiving module and the test parameter setting module; the ultrasonic excitation and receiving module is used for exciting the biological hard tissue sample to generate resonance and realizing resonance frequency spectrum receiving; the test parameter setting module is used for setting ultrasonic resonance spectrum experiment parameters;

wherein:

the pulse generating circuit module is used for generating an electric signal (frequency sweep signal) with specific frequency range and power; the ultrasonic probe is a group of (2) light shear wave ultrasonic probes, the first ultrasonic probe is electrically connected with the pulse generation circuit module, and the surface of the probe is placed upwards and used for exciting the biological hard tissue to resonate; acting on the biological hard tissue material to be detected; the second ultrasonic probe is placed with the surface downward and is used for receiving the resonance frequency spectrum of the biological hard tissue material sample. The signal processing circuit module is electrically connected with the second ultrasonic probe, amplifies the output signal, performs analog-digital conversion and realizes storage of the resonance frequency spectrum of the biological hard tissue sample;

the excitation parameter setting module is used for setting parameters such as excitation intensity, frequency range, frequency resolution and the like; the receiving parameter setting module is used for setting parameter settings such as receiving bandwidth and the like.

The advantages of the invention include:

the invention realizes the accurate measurement of the resonance frequency of the biological hard tissue material and provides a more advanced technical means for the accurate description of the mechanical properties of the biological hard tissue.

The details of the structure and operation of the present invention are described in the following with reference to the accompanying drawings and examples.

As shown in FIG. 1, the measurement platform suitable for the ultrasonic resonance spectrum of the biological hard tissue material sample according to one embodiment of the invention comprises an ultrasonic excitation and receiving module and a test parameter setting module.

The ultrasonic excitation and receiving module mainly comprises:

the ultrasonic probe comprises a pulse generating circuit module 101, a first ultrasonic probe 102, a second ultrasonic probe 103 and a signal processing module 104. To illustrate the specific structure of the platform designed according to the present invention in more detail, a small-sized sample of biological hard tissue (e.g., dentin, etc.) to be tested 105 is added to this section.

The pulse generating circuit module 101 is used for generating a frequency sweep signal with a specific frequency range and power;

the first ultrasonic probe 102 is connected with the pulse generation circuit module 101, and the surface of the probe is placed upwards and used for exciting the biological hard tissue sample to resonate;

the second ultrasonic probe 103 is placed face down for receiving the biological hard tissue material sample resonance spectrum.

The signal processing circuit module 104 is connected with the second ultrasonic probe, amplifies the output signal, performs analog-digital conversion, and realizes storage of the resonance frequency spectrum of the biological hard tissue sample;

the test parameter setting module comprises:

an excitation parameter setting module 201 and a receiving parameter setting module 202.

The excitation parameter setting module 201 sets the excitation parameters as follows: the ultrasonic excitation frequency is set to be 100-700kHz, and the frequency sweep frequency increasing mode is set to be linear; in order to avoid the large influence of the viscoelasticity of the biological hard tissue sample on the ultrasonic resonance spectrum, the frequency resolution is set to be 50-100 Hz;

the reception parameter setting module 202 sets the reception bandwidth to 30Hz or 100Hz depending on the samples.

An example of a resonance spectrogram obtained by applying an embodiment of the ultrasonic resonance spectroscopy measurement of a sample of biological hard tissue material according to the present invention is shown in fig. 2.

The dentin sample 105 selected for this example of the invention was 5.696X 3.620X 2.704mm in size³The mass was 111 mg.

The working process according to one embodiment of the invention is as follows:

as shown in fig. 3, a small-sized biological hard tissue sample 105 (such as dentin, etc.) to be tested is firstly placed between a first ultrasonic probe 102 and a second ultrasonic probe 103, and the sample 105 is ensured to be in a free surface boundary condition state as much as possible; the excitation parameter setting module sets 201 excitation parameters such as excitation intensity, frequency range, frequency resolution and the like; a sweep frequency signal in a specific frequency range is generated by the pulse generation circuit module 101, and the dentin sample 105 to be detected is excited by the first ultrasonic probe 102 to generate resonance; the resonance spectrum output signal of the dentin sample 105 to be detected is received by the second ultrasonic probe 104, the resonance spectrum signal is input into the signal processing module 105 after the parameters such as receiving bandwidth are set by the receiving parameter setting module 202, the signal processing module 104 completes signal amplification and analog-digital conversion, and the resonance spectrum storage of the dentin sample 105 to be detected is realized.

It should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the same. Although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.