阅读说明：本技术 一种基于全息光学的多色成像系统及全息光镊装置 (Multicolor imaging system based on holographic optics and holographic optical tweezers device ) 是由 娄凯 温维佳 于 2021-09-03 设计创作，主要内容包括：本申请公开了基于全息光学的多色成像系统及全息光镊装置,包括：激光器用于发出光捕获所用激光；偏振调制部用于将激光器发出的激光进行偏振态调制,以实现激光强度的控制；空间光调制器用于对来自偏振调制部的光进行光场调制,调制后的光场经物镜紧聚焦后形成光镊。系统包括：多波长光模块用于激发不同波段染料,对样品进行多色成像与追踪；显微镜,放大和观察样品的平台；图像采集模块用于光力矩分析和多色荧光成像；控制终端用于控制全息光镊模块、读取图像采集模块的成像信息和控制显微镜中的电动机械快门。通过本申请解决了光镊系统在显微成像方面能力局限的问题,利用全息光镊捕获和移动样品,并进行样本多维结构与动态分析。(The application discloses polychrome imaging system and holographic optical tweezers device based on holographic optics includes: the laser is used for emitting laser used for light capture; the polarization modulation part is used for modulating the polarization state of the laser emitted by the laser so as to realize the control of the laser intensity; the spatial light modulator is used for carrying out light field modulation on light from the polarization modulation part, and the modulated light field is tightly focused by the objective lens to form the optical tweezers. The system comprises: the multi-wavelength optical module is used for exciting dyes in different wave bands and carrying out multi-color imaging and tracking on a sample; a microscope, a stage to magnify and observe the sample; the image acquisition module is used for optical moment analysis and multicolor fluorescence imaging; the control terminal is used for controlling the holographic optical tweezers module, reading imaging information of the image acquisition module and controlling an electromechanical shutter in the microscope. The problem of the capacity limitation of the optical tweezers system in the aspect of microscopic imaging is solved through the application, the holographic optical tweezers are used for capturing and moving the sample, and the multidimensional structure and the dynamic analysis of the sample are carried out.)

1. A holographic optical tweezers device, comprising:

a laser for emitting laser light;

the polarization modulation part is used for modulating the polarization state of the laser emitted by the laser so as to realize the control of the laser intensity;

and the spatial light modulator is used for carrying out light field modulation on the laser from the polarization modulation part, wherein the modulated light field forms optical tweezers on a focal plane of a microscope objective lens.

2. The apparatus according to claim 1, wherein the polarization modulation section comprises:

the optical isolator is used for isolating the reflected and reversed laser and enabling the laser emitted by the laser to be linearly polarized;

a half-wave plate which rotates the light polarization direction of the linearly polarized light by rotation;

the beam expander expands the diameter of the laser beam of the linearly polarized light emitted by the half-wave plate;

the light passing through the beam expander is incident to the polarization beam splitter or the polarizing plate, wherein the polarizing plate is used for outputting light in a preset polarization direction, and the polarization beam splitter divides the incident light into two beams of light with mutually orthogonal polarization directions for output.

3. The apparatus according to claim 2, wherein the polarization modulation section further comprises:

and the first reflecting mirror and the second reflecting mirror are arranged between the half-wave plate and the beam expander, wherein emergent light passing through the first transmitting mirror and the second transmitting mirror is parallel to incident light entering the first reflecting mirror and the second reflecting mirror and has an opposite direction.

4. The apparatus of claim 1, further comprising:

the polarization modulation part is arranged on the first reflecting mirror, the second reflecting mirror is arranged on the second reflecting mirror, and the polarization modulation part is arranged on the first reflecting mirror.

5. The apparatus of any one of claims 1 to 4, further comprising:

and emergent light of the spatial light modulator is emitted after passing through the lens.

6. The apparatus of claim 5, wherein the lens comprises:

the lens comprises a first lens, a second lens and a spatial isolator arranged between the first lens and the second lens, wherein the spatial isolator is used for filtering out zero-order diffraction light.

7. A multicolor imaging system based on holographic optical tweezers, comprising:

the multi-wavelength optical module is used for emitting exciting light with multiple wavelengths, wherein the light emitted by the multi-wavelength optical module is used for exciting a fluorescent dye to generate multiple kinds of fluorescence;

holographic optical tweezers device according to any one of claims 1 to 6, wherein the optical tweezers are adapted to capture and/or move particles that are excited to produce fluorescence;

and the first dichroic mirror is used for combining the emergent light of the multi-wavelength LED optical module and the emergent light of the holographic optical tweezers device.

8. The system of claim 7, further comprising:

the microscope comprises a first microscope objective, a sample stage, a second microscope objective, an electromechanical shutter, a white light source, a second dichroic mirror and a tube lens. The first microscope objective is used for focusing incident laser to form a multi-optical trap structure and collecting images; the sample table is used for placing and fixing a sample; the white light source is an illumination light source for observing a sample; the electromechanical shutter is used for controlling the exposure time; the second microscope objective focuses the white light source to the sample stage; the second dichroic mirror reflects the holographic optical tweezers laser and the multi-wavelength exciting light into the first microscope objective and transmits light of a wave band used for analyzing the fluorescence and optical moment of multicolor imaging; the tube lens converges the microscope output light which transmits the second dichroic mirror to the image acquisition module;

the image acquisition module is used for ultrafast image acquisition to analyze light moment and multicolor fluorescence microscopic imaging;

and the control terminal is used for controlling the holographic optical tweezers device, reading the imaging information of the image acquisition module and controlling an electromechanical shutter in the microscope.

9. The system of claim 7, wherein the light from the multi-wavelength light module is LED excitation light.

Technical Field

The application relates to the field of light, in particular to a multicolor imaging system based on holographic optics and a holographic optical tweezers device.

Background

The optical tweezers, i.e. the single-beam gradient force optical trap, is a potential well which is formed based on the interaction of scattering force and radiation pressure gradient force and can capture particles in the whole Mie and Rayleigh scattering ranges. What acts as a trap for the particle is the gradient force, which must overcome the scattering force in order to stably confine the particle in the optical field well. It is generally necessary to use a microscope objective with a large numerical aperture to highly converge the laser beam so as to generate a strong enough gradient force to achieve particle trapping. In the fields of soft materials, life science, biomedicine and the like, the optical tweezers technology can be combined with the optical microscope objective technology to realize the observation, capture and operation of single particles, but the microscope optical tweezers device is not applied to high-sensitivity quantitative detection.

For optical tweezers systems in optical path structures involved in the detection fields of soft substances, life sciences, biomedicine and the like, the system in the prior art has limited capability of generating optical traps for trapping particles, and the application of the optical tweezers is influenced.

Disclosure of Invention

The embodiment of the application provides a multicolor imaging system based on holographic optics and a holographic optical tweezers device, and aims to at least solve the problem that the capabilities of an optical tweezers system based on a microscope and a multi-wavelength fluorescence imaging system in the prior art are limited.

According to an aspect of the present application, there is provided a holographic optical tweezers device comprising: a laser for emitting laser light; the polarization modulation part is used for modulating the polarization state of the laser emitted by the laser so as to realize the control of the laser intensity; and the spatial light modulator is used for carrying out light field modulation on the light from the polarization modulation part, wherein the modulated light field forms optical tweezers after passing through the first microscope objective.

Further, the polarization modulation section includes: the optical isolator is used for isolating the reflected and reversed laser and enabling the laser emitted by the laser to be linearly polarized; a half-wave plate which rotates the light polarization direction of the linearly polarized light by rotation; the beam expander expands the diameter of the laser beam of the linearly polarized light emitted by the half-wave plate; the light passing through the beam expander is incident to the polarization beam splitter or the polarizing plate, wherein the polarizing plate is used for outputting light in a preset polarization direction, and the polarization beam splitter divides the incident light into two beams of light with mutually orthogonal polarization directions for output.

Further, the polarization modulation section further includes: and the first reflecting mirror and the second reflecting mirror are arranged between the half-wave plate and the beam expander, wherein emergent light passing through the first transmitting mirror and the second transmitting mirror is parallel to incident light entering the first reflecting mirror and the second reflecting mirror and has an opposite direction.

Further, the apparatus further comprises: the polarization modulation part is arranged on the first reflecting mirror, the second reflecting mirror is arranged on the second reflecting mirror, and the polarization modulation part is arranged on the first reflecting mirror.

Further, the apparatus further comprises: and emergent light of the spatial light modulator is emitted after passing through the lens.

Further, the lens includes: the optical lens comprises a first lens, a second lens and a space isolator arranged between the first lens and the second lens, wherein the space isolator is used for filtering out zero-order light.

According to another aspect of the present application, there is also provided a holographic optical tweezers-based multicolor imaging system, comprising: the multi-wavelength optical module is used for emitting exciting light with multiple wavelengths, wherein the light emitted by the multi-wavelength optical module is used for exciting a fluorescent dye to generate multiple kinds of fluorescence; the holographic optical tweezers device, wherein the optical tweezers are used for capturing and/or moving the particles which are excited to generate fluorescence; and the first dichroic mirror is used for combining the emergent light of the polychromatic light module and the emergent light of the holographic optical tweezers device.

Further, the multicolor imaging system further comprises a microscope, wherein the microscope comprises a first microscope objective, a sample stage, a second microscope objective, an electromechanical shutter, a white light source, a second dichroic mirror and a tube lens. The first microscope objective is used for focusing incident laser to form a multi-optical trap structure and collecting images; the sample table is used for placing and fixing a sample; the white light source is an illumination light source for observing a sample; the electromechanical shutter is used for controlling the exposure time; the second microscope objective focuses the white light source to the sample stage; the second dichroic mirror reflects the holographic optical tweezers laser and the multi-wavelength excitation light into the first microscope objective and transmits light of a waveband used for analyzing the fluorescence and optical moment of multicolor imaging; the tube lens converges the microscope output light which transmits the second dichroic mirror to the image acquisition module;

further, the multi-color imaging system further comprises an image acquisition module for ultrafast image acquisition to analyze the light moment and the multi-color fluorescence microscopic imaging;

further, the multicolor imaging system also comprises a control terminal, which is used for controlling the holographic optical tweezers module, reading the imaging information of the image acquisition module and controlling an electromechanical shutter in the microscope.

Further, the excitation light emitted by the multi-wavelength light module is LED light.

In the embodiment of the application, a laser is adopted to emit laser for holographic optical tweezers; the polarization modulation part is used for carrying out polarization state modulation and beam splitting on the laser emitted by the laser to obtain two beams of light with mutually orthogonal polarization directions; and the spatial light modulator is used for carrying out light field modulation on the light from the polarization modulation part, wherein the modulated light field is focused by the first microscope objective to form the optical tweezers. The problem that the capacity of a microscope-based optical tweezers system and a multi-wavelength fluorescence imaging system is limited in the prior art is solved, so that the holographic optical tweezers can be used for capturing and moving particles, and the spatial structure and the dynamic state of a sample are analyzed.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the application and, together with the description, serve to explain the application and are not intended to limit the application. In the drawings:

FIG. 1 is a schematic diagram of a holographic optics based multicolor imaging system and holographic optical tweezers device system according to embodiments of the present application;

fig. 2 is a block diagram of a multicolor imaging system based on holographic optics and a holographic optical tweezers device system according to an embodiment of the present application.

In the above drawings, the parts corresponding to the reference numerals are as follows: the device comprises a laser 1, an optical isolator 2, a half-wave plate 3, a beam expander 4, a polaroid 5, a spatial light modulator 6, a lens 7, a spatial isolator 8, a lens 9, a multi-wavelength LED 10, a first dichroic mirror 11, a second dichroic mirror 12, a first microscope objective 13, a sample stage 14, a second microscope objective 15, an electromechanical shutter 16, a white light source 17, a tube mirror 18, an electrically-controlled switching mirror 19, an ultrafast camera 20, a camera link 21, an optical filter 22, a high-gain camera 23 and a control terminal 24.

Detailed Description

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present application will be described in detail below with reference to the embodiments with reference to the attached drawings.

In this embodiment, there is provided a holographic optical tweezers device incorporating multicolor fluorescence microscopy imaging, comprising: a laser 1 for emitting laser light for capturing fine particles; the polarization modulation part is used for modulating the polarization state of the laser emitted by the laser so as to realize the control of the laser intensity; and the spatial light modulator 6 is used for carrying out light field modulation on the light from the polarization modulation part, wherein the modulated light field forms the optical tweezers after being tightly focused by the first microscope objective.

By the device, the optical tweezers formed by holographic light can be generated, and the problem of limited capability of a microscope-based optical tweezers system in the prior art is solved, so that the holographic optical tweezers can be used for capturing and moving particles, and the spatial structure and the dynamic state of a sample can be analyzed through multicolor fluorescence microscopic imaging.

In an alternative embodiment, the spatial light modulator 6 may be under active control, and it may modulate a parameter of the optical field through liquid crystal molecules, for example, modulate the amplitude of the optical field, modulate the phase through the refractive index, modulate the polarization state through the rotation of the polarization plane, or implement the conversion of incoherent-coherent light, so as to write a certain information into the optical wave, thereby achieving the purpose of optical wave modulation. The optical tweezers can conveniently load information into a one-dimensional or two-dimensional optical field, and the loaded optical tweezers are the optical tweezers to be obtained.

The polarization modulation part (or called modulation part) may include various structures, for example, in an alternative embodiment, the polarization modulation part may include: the optical isolator 2 is used for isolating the reflected and reversed laser and enabling the laser emitted by the laser to be linearly polarized; the half-wave plate 3 rotates the light polarization direction of the linearly polarized light through rotation; the beam expander 4 expands the diameter of the laser beam of the linearly polarized light emitted by the half-wave plate; and the light passing through the beam expander is incident to the polarization beam splitter 5 or the polarizing plate 5, wherein the polarizing plate 5 is used for outputting light with a predetermined polarization direction, and the polarization beam splitter 5 divides the incident light into two beams of light with mutually orthogonal polarization directions for outputting. In one embodiment, the polarization modulation section can perform functions of polarization, polarization rotation, and polarization beam splitting.

The components in this alternative embodiment are described separately below. The primary function of the optical isolator 2 is to isolate the back-reflected laser light from damaging the laser. In another alternative, a polarization-dependent optical isolator may be used, where the polarization direction of the laser light emitted by the laser itself is arbitrary and stable, and the polarization-dependent optical isolator outputs the input light with arbitrary polarization as linearly polarized light. The half-wave plate 3 can rotate linearly polarized light, the included angle between the polarization direction of the light and the optical axis of the half-wave plate is a, and then the included angle between the polarization direction of the linearly polarized light and the optical axis of the half-wave plate is 2a after the linearly polarized light passes through the half-wave plate. The beam expander 4 makes the beam diameter larger, and there may be two kinds. The polarization beam splitter 5 splits incident light in any polarization direction into two predetermined mutually orthogonal polarization directions, and finally outputs two orthogonal beams of light; the polarizing plate allows only a light component of a certain polarization direction to pass therethrough and completely blocks a light component orthogonal to the polarization direction with respect to an incident light of an arbitrary polarization direction. It is apparent that the intensity of light output through a polarizing beam splitter or polarizer will change as the polarization direction of the incident light is rotated.

Through above-mentioned several parts, the laser damage laser instrument that the optoisolator exported linearly polarized light and the separation reflection falls back, linearly polarized light gets into the half-wave plate and is rotated, through rotating the half-wave plate (rotating the optical axis of half-wave plate promptly simultaneously), thereby realize rotating the polarization direction of linearly polarized light wantonly, the linearly polarized light that passes through polarization rotation gets into polarization beam splitter or polaroid after expanding the beam, its purpose is the same all for selecting the light component of a certain polarization direction, because only a part light component is screened, thereby the luminous intensity has been attenuated, cooperate rotatory half-wave plate, intensity modulation has been realized.

In order to reduce the occupied space of the holographic optical tweezers device, the optical path direction may be changed in the polarization modulation section, and in this alternative embodiment, the polarization modulation section may further include: and the first reflecting mirror and the second reflecting mirror are arranged between the half-wave plate and the beam expander, wherein emergent light passing through the first transmitting mirror and the second transmitting mirror is parallel to incident light entering the first reflecting mirror and the second reflecting mirror and has opposite direction.

Similarly, the direction of the optical path may also be changed in this holographic optical tweezers device, and in this alternative embodiment, a third mirror and a fourth mirror disposed between the polarization modulation section and the spatial light modulator may be added to the device, where the outgoing light passing through the third mirror and the fourth mirror is parallel to and in the opposite direction to the incoming light entering the third mirror and the fourth mirror.

The outgoing laser of the spatial light modulator may also be optimized, that is, in an alternative embodiment, the holographic optical tweezers device may further include: and the emergent light of the spatial light modulator is emitted after passing through the lens. For example, the lens may include: a first lens 7 and a second lens 9, and a spatial isolator 8 disposed between the first lens 7 and the second lens 9, wherein the spatial isolator 8 is configured to reject zero order diffracted light.

In this embodiment, a holographic optical tweezers-based multicolor imaging system is provided, comprising: the multi-wavelength excitation optical module is used for emitting LED light with multiple wavelengths, wherein the light emitted by the multi-wavelength excitation optical module is used for exciting a fluorescent dye to generate multi-color fluorescence; the holographic optical tweezers device, wherein the optical tweezers are used for capturing and/or moving the sample excited to generate fluorescence or the particles adjacent to the sample generating fluorescence; and the first dichroic mirror is used for combining the emergent light of the multi-wavelength excitation light module and the emergent laser of the holographic optical tweezers device. The excitation light source emitted by the multi-wavelength excitation light module can be an LED light source.

The system may further comprise: and (4) a microscope. The microscope comprises a first microscope objective, a sample stage, a second microscope objective, an electric mechanical shutter, a white light source, a second dichroic mirror and a tube lens. The first microscope objective is used for focusing incident laser to form a multi-optical trap structure and collecting images; the sample table is used for placing and fixing a sample; the white light source is an illumination light source for observing the sample; the electromechanical shutter is used for controlling the exposure time; the second microscope objective collects the white light source to the sample stage; the second dichroic mirror reflects the holographic optical tweezers laser and the multi-wavelength exciting light into the first microscope objective and transmits light of a wave band used for the fluorescence and optical moment analysis of multicolor imaging; the tube lens converges the microscope output light which transmits the second dichroic mirror to the image acquisition module; the image acquisition module is used for acquiring image information; and the control terminal is used for controlling the holographic optical tweezers module, reading the imaging information of the image acquisition module and controlling an electromechanical shutter in the microscope.

For better photographing the fluorescence, the system may further include a filter 22 for filtering the fluorescence transmitted through the dichroic mirror (cutting off the excitation light);

this is described below in connection with an alternative embodiment. In this alternative embodiment, fig. 1 is a schematic diagram of a holographic optical tweezers imaging system according to an embodiment of the application, as shown in fig. 1, the system comprising: the system comprises a multi-wavelength excitation light module, a holographic optical tweezers module, an image acquisition module, a control terminal and a microscope, wherein after being focused by a first microscope objective, the holographic optical tweezers module provides an optical trap for capturing particles, and the multi-wavelength excitation light module provides multi-color LED light for exciting fluorescence; in addition, the microscope provides a platform for amplifying and observing the sample, and the image acquisition module is used for acquiring image information; after the signal is collected, the control terminal is used for reading imaging information sent back by the camera and controlling the holographic optical tweezers. The following is a block diagram.

Multi-wavelength excitation optical module

The multi-wavelength excitation optical module outputs LED excitation light with various wavelengths, and the light beam is uniform and collimated and is used for exciting the fluorescent dye to emit fluorescence.

The multi-wavelength excitation optical module may have various structures, and in this embodiment, a preferable structure is provided, in which N monochromatic light sources (N may be 4 or 6) are included, where the N monochromatic light sources include: the light source comprises a first light source and at least one second light source, wherein the light emitting direction of the first light source is a first direction, the light emitting direction of the second light source is a second direction or a third direction, the first direction is the light emitting direction of the multicolor light source, the second direction is a direction perpendicular to the first direction, the third direction is a direction parallel to the first direction, the wavelength of light emitted by each monochromatic light source is different, and all or part of N monochromatic light sources emit light simultaneously; the monochromatic light source is an LED light source. The M dichroic mirrors include: at least one first dichroic mirror disposed in a first direction and at least one second dichroic mirror disposed in a second direction; the first dichroic mirror transmits the light of the first light source and reflects the light of the second light source emitted in the second direction to the first direction; the second dichroic mirror transmits light of the second light source emitted in the second direction and reflects light of the second light source emitted in the third direction to the second direction. In order to improve the quality of the light, at least one of the following devices is arranged before the light is combined: the N Fresnel lenses are respectively arranged between each monochromatic light source and the dichroic mirror closest to the monochromatic light source; and each optical filter is arranged between each Fresnel lens and the dichroic mirror closest to the Fresnel lens. For the light after beam combination, the quality can also be improved by at least one of the following means: and the light beams of the N light sources pass through the plano-convex lens after being combined. And the light beams of the N light sources pass through the biconcave lens after being combined. As an alternative embodiment, in the case where both the plano-convex lens and the biconcave lens are provided, the combined beam of light passes through the plano-convex lens before passing through the biconcave lens, e.g., the combined beam of light passes through the plano-convex lens before passing through the biconcave lens when spatially output.

Holographic optical tweezers module

As shown in fig. 2, 1 is a laser, which is controlled by a control terminal and emits laser light into an optical isolator 2; the optical isolator 2 is used for isolating the reflected and reversed laser to prevent the laser from being damaged and simultaneously converting the laser into linearly polarized light; then laser is injected into 3, 3 which are half-wave plates, the polarization direction of light is correspondingly rotated by rotating the half-wave plates, and the rotating angle satisfies the following relation: the included angle between the polarization direction of emergent light and the polarization direction of incident light is twice the included angle between the polarization direction of incident light and the main shaft of the half-wave plate; the linearly polarized light after rotation enters the beam expander 4 after being reflected twice by the two reflectors, and the diameter of the laser beam is enlarged; linearly polarized light after beam expansion enters 5, 5 is a polarizing film or a polarizing beam splitter, the polarizing film can only pass light in a certain polarization direction, the polarizing beam splitter divides any incident light into two beams of light with mutually orthogonal polarization directions for output, and 3 and 5 are matched to perform laser intensity attenuation adjustment; the emergent laser enters a 6 spatial light modulator after being reflected by two reflectors, the spatial light modulator is controlled by a control terminal to modulate a light field, and finally a plurality of focusing light spots are generated in the light field at a sample to capture particles or movable focusing light spots are generated to move the particles, namely, the holographic optical tweezers are formed; the emergent light of the spatial light modulator is equivalent to one-time Fourier transform through the lens 7, the spatial isolator 8 plays a role of a baffle to filter out zero-order light (interference item), and the collimated light beam is emitted after passing through the lens 9.

Microscope

As shown in fig. 2, the multi-wavelength LED light and the holographic optical tweezers module emit laser light to enter the second dichroic mirror 12, the second dichroic mirror 12 reflects the multi-color LED light and the holographic optical tweezers laser light, and transmits a plurality of fluorescent lights generated by exciting the fluorescent dye by the multi-color LED light; the reference numeral 13 is a first microscope objective, the lenses 7 and 9 of the holographic optical tweezers module form a 4f system, and the modulated optical field forms an optical trap at the sample stage 14 to capture and move particles; 14 is a sample table for placing a sample to be observed; 15, a second microscope objective lens collects the white light source to the sample stage; 16 is an electromechanical shutter for controlling the exposure time; 17 is a white light source, which is an illumination light source for observing a sample; and 18 is a tube lens, and the optical signals are converged into the image acquisition module.

Image acquisition module

The signal acquisition mainly has two functions: the ultra-fast camera combined with the camera connecting plate is used for measuring optical moment; the high-gain camera is used for multicolor fluorescence microscopic imaging.

Control terminal

The control terminal is generally a computer, and communicates with the holographic optical tweezers module to realize real-time optical field modulation, communicates with the high-gain camera 23 in the image acquisition module to realize real-time multicolor imaging, and communicates with the ultrafast camera 20 in the image acquisition module and the electromechanical shutter 16 in the microscope to realize optical moment measurement.

The holographic optical tweezers can be used for capturing and moving particles by the above embodiment, and multicolor imaging can also be performed. The particle capture and movement, multicolor imaging, are all in real time in the above embodiments.

In the embodiment, the optical moment measuring function is also provided, light emitted by the white light source 17 of the illumination light source enters the sample stage 14 through the second microscope objective 15, and light with a predetermined waveband serving as the illumination light source enters the ultra-fast camera 20 after passing through the first microscope objective 13. Preferably, the light of the predetermined wavelength band passes through the first microscope objective 13, then transmits through the second dichroic mirror 12, the tube mirror 18, and is reflected by the reflecting mirror 19 to enter the ultrafast camera 20.

As shown in fig. 2, in multicolor imaging, a high-gain camera is used, an ultra-fast camera and a camera connecting board are used in optical moment measurement, and a white light source is used in optical moment measurement.

When multicolor fluorescence microscopy imaging:

the multi-wavelength LED light and the laser emitted by the holographic optical tweezers module enter the dichroic mirror 12, the dichroic mirror 12 reflects the multi-wavelength LED light and the holographic optical tweezers laser, and the multi-wavelength LED light and the holographic optical tweezers laser enter the sample stage 14 after passing through the first microscope objective 13. Note that lenses 7 and 9 of the holographic optical tweezer module together form a 4f system, and the holographic optical tweezer module modulates the optical field to form multi-optical traps at the sample stage 14 to capture and move particles in real time; note that the second microscope objective 15 and the electromechanical shutter 16 do not work during multicolor imaging, the white light source 17 does not emit light, the fluorescent dye in the sample is excited by the multi-wavelength LED light to emit corresponding multi-wavelength fluorescence, the multi-wavelength fluorescence returns to the first microscope objective 13, then transmits through the dichroic mirror 12, passes through the tube lens 18, is reflected by the reflector 19, and enters the high-gain camera 23 for imaging.

When measuring the optical moment:

the multi-wavelength LED light source 10 is closed, the white light source 17 is opened, enters the sample stage 14 through the electromechanical shutter 16 and the second microscope objective 15 and serves as a lighting source, multi-band light passes through the first microscope objective 13, the transmission dichroic mirror 12, the tube mirror 18, the reflection mirror 19 and the ultrafast camera 20 to be detected and imaged, the number of images collected by the ultrafast camera 20 per second is greatly increased through the camera link plate 21, and the control terminal 24 processes the collected images and finally calculates light torque.

The control terminal is generally a computer, and is used for controlling the holographic optical tweezers and communicating with the camera to process imaging information.

In the embodiment, the holographic optical tweezers can be used for capturing and moving the particles, wherein the capturing and moving of the particles and the multicolor imaging are real-time, and the embodiment can not only perform the multicolor imaging but also perform the optical moment measurement.

The above are merely examples of the present application and are not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.