阅读说明：本技术 用于重组多种逆转录病毒毒株中的不对称靶位点的良好耐受且高度特异性的修整重组酶 (Well-tolerated and highly specific tailored recombinases for recombining asymmetric target sites in multiple retroviral strains ) 是由 J·豪贝尔 J·凯姆尼茨 F·布希霍尔茨 J·卡尔平斯基 于 2015-09-01 设计创作，主要内容包括：本发明涉及用于重组多种逆转录病毒毒株中的不对称靶位点的良好耐受且高度特异性的修整重组酶。本发明涉及制备编码良好耐受且高度特异性修整重组酶的表达载体的方法,以及获得的表达载体,用这些转染的细胞,表达的重组酶和包含所述表达载体、细胞和/或重组酶的药物组合物,所述修整重组酶能够重组可以插入宿主细胞的基因组中的多种逆转录病毒毒株的原病毒DNA的长末端重复(LTR)内的不对称靶序列。药物组合物可用于,例如,治疗和/或预防逆转录病毒感染,特别是HIV感染。具体而言,本发明涉及良好耐受且高度特异性修整重组酶,其能够组合超过90％的HIV-1毒株中的不对称靶序列,由此切除HIV-1序列和编码它们的表达载体。(The present invention relates to well-tolerated and highly specific tailored recombinases for recombining asymmetric target sites in a variety of retroviral strains. The present invention relates to a method for the preparation of expression vectors encoding well-tolerated and highly specific tailored recombinases capable of recombining asymmetric target sequences within the Long Terminal Repeat (LTR) of proviral DNA of multiple retroviral strains that can be inserted into the genome of a host cell, as well as to the obtained expression vectors, cells transfected with these, expressed recombinases and pharmaceutical compositions comprising said expression vectors, cells and/or recombinases. The pharmaceutical compositions are useful, for example, in the treatment and/or prevention of retroviral infections, particularly HIV infections. In particular, the present invention relates to well-tolerated and highly specific tailored recombinases that are capable of combining asymmetric target sequences in more than 90% of HIV-1 strains, thereby excising HIV-1 sequences and the expression vectors encoding them.)