阅读说明：本技术 用于分析核酸扩增反应的分析方法和系统 (Analysis method and system for analyzing nucleic acid amplification reaction ) 是由 R.诺贝尔 于 2015-08-26 设计创作，主要内容包括：本发明涉及用于分析核酸扩增反应的分析方法和系统。本发明涉及一种以在较宽范围的分析物浓度里改善通过实时核酸扩增反应RNR(诸如聚合酶链式反应PCR)量化的线性(精确性和/或准确性)和/或限制存在干扰性物质的效应为目的的分析方法,其通过以与增长曲线与包含至少两个不同阈值水平的在RNR循环范围里的组合阈值函数(CTF)的交叉对应的循环数目测定RNR的量化循环数目(Cq)进行,所述量化循环数目(Cq)指示增长曲线的定量和/或定性分析结果,所述增长曲线指示RNR循环范围里对于RNR的每个扩增反应循环,分析物的荧光发射强度。(The present invention relates to an analysis method and system for analyzing a nucleic acid amplification reaction. The invention relates to an analytical method with the aim of improving the linearity (accuracy and/or precision) of the quantification of RNRs by real-time nucleic acid amplification reactions (such as polymerase chain reaction PCR) and/or limiting the effect of the presence of interfering substances in a wide range of analyte concentrations by determining the quantification cycle number (Cq) of RNRs, which indicates the result of the quantitative and/or qualitative analysis of the growth curve, by the cycle number corresponding to the intersection of the growth curve with a Combined Threshold Function (CTF) in the RNR cycle range comprising at least two different threshold levels, which indicates the intensity of the fluorescence emission of the analyte for each amplification reaction cycle of RNRs in the RNR cycle range.)