阅读说明：本技术 一种定向形貌胶原支架制备方法及其制备设备 (Preparation method and preparation equipment of collagen scaffold with directional morphology ) 是由 何一波 刘小娇 陈宇 于 2021-08-12 设计创作，主要内容包括：本发明属于胶原支架制备技术领域,且公开了一种定向形貌胶原支架的制备方法,包括如下步骤：取动物的上脊软组织并提取胶原蛋白,然后放在准备好的模具中,加入事先配置好的乙酸溶液,使得胶原蛋白乙酸溶液中,取pH试纸并测量模具中的pH值,使之控制在4～5之间；用实验棒搅拌模具中的胶原蛋白溶液使之混合成型,然后冷藏30分钟。本发明通过开设有制冷孔增加定型筒的表面积,进而加快模具的受冷速度,通过设置有固定环和缩紧环以受冷后冷缩的形式向内勒动缓冲环,使得模具被缓冲环压紧,被压紧后的模具会整体向内收缩,同时,会使得模具的内壁向内侧凹陷,进而使之与其内部的胶原支架胶体更加贴合,从而使得胶原胶体的冷冻效率更高。(The invention belongs to the technical field of preparation of collagen scaffolds, and discloses a preparation method of a collagen scaffold with an oriented morphology, which comprises the following steps: taking the upper spine soft tissue of an animal, extracting collagen, then placing the extracted collagen in a prepared mould, adding a prepared acetic acid solution into the prepared mould, taking pH test paper, and measuring the pH value in the mould to control the pH value to be between 4 and 5; the collagen solution in the mold was stirred with a laboratory stick and mixed for molding, and then refrigerated for 30 minutes. According to the invention, the surface area of the shaping cylinder is increased by arranging the refrigeration hole, so that the cooling speed of the mold is accelerated, the buffer ring is pulled inwards in a cold contraction mode by arranging the fixing ring and the contraction ring, so that the mold is pressed by the buffer ring, the pressed mold can integrally contract inwards, meanwhile, the inner wall of the mold is sunken inwards, and further the mold is more attached to the collagen support colloid in the mold, so that the refrigeration efficiency of the collagen colloid is higher.)

1. A preparation method of a collagen scaffold with oriented morphology comprises the following steps:

taking the upper spine soft tissue of an animal, extracting collagen, then placing the extracted collagen in a prepared mould, adding a prepared acetic acid solution into the prepared mould, taking pH test paper, and measuring the pH value in the mould to control the pH value to be between 4 and 5;

stirring the collagen solution in the mould by using an experimental bar to mix and form, and then refrigerating for 30 minutes, wherein the refrigerating temperature is set to be 4 ℃;

placing a mould filled with the refrigerated and molded collagen mixed solution in a buffer ring, so that the placing block is clamped in the placing groove, and the outer wall of the mould is in contact with the buffer ring and extrudes the buffer ring;

injecting a frozen substance into the placing table, covering the mounting cover on the placing table, clamping the outer sealing ring on the outer sealing groove, and driving the frozen substance to rise upwards by the lifting ring which extends into the placing table;

the mounting cover moving downwards drives the pressing ring to move downwards and wrap the outer surface of the connecting block to form a sealing effect, and simultaneously drives the sealing mechanism to extrude and move into the die;

at this time, the inside of the placing table was frozen in a directional manner, and after waiting for 40 minutes, the collagen scaffold was molded.

2. The utility model provides a preparation equipment of directional appearance collagen support, includes protective housing (1), places platform (2) and installation lid (3), its characterized in that: the bottom of the mounting cover (3) is fixedly provided with a sealing mechanism (6) and a pressing ring (7), a shaping cylinder (9) is arranged in the middle of the inner cavity of the placing table (2), an inner groove (10) is arranged on the inner wall of the shaping cylinder (9), the inner part of the inner groove (10) is movably clamped with a fixed ring (11) and a contraction ring (12) in a staggered way, a buffer ring (13) is movably sleeved in the fixed ring (11) and the tightening ring (12), a placing groove (15) is arranged at the top of the shaping cylinder (9), a mould (14) is movably clamped in the placing groove (15), an outer sealing groove (18) is formed in the top of the placing table (2), an outer sealing ring (19) is hermetically sleeved in the outer sealing groove (18), the top of the outer sealing ring (19) is fixedly connected with the mounting cover (3), and the bottom of the mounting cover (3) is fixedly provided with a lifting ring (5).

3. The apparatus for preparing a collagen scaffold with directional morphology according to claim 2, wherein: the protective housing (1) is made by thermal insulation material, place the inside of platform (2) activity cup joint at protective housing (1), sealing mechanism (6) fixed mounting is at the middle part of installation lid (3) bottom surface, the bottom of sealing mechanism (6) extends to the inside of mould (14), the inside activity joint of standing groove (15) has places piece (16), the top fixed mounting who places piece (16) has connecting block (17), the medial surface of connecting block (17) bottom and the top fixed connection of mould (14), the equal fixed mounting in the left and right sides of installation lid (3) has carrying handle (4), the bottom of carrying handle (4) has been seted up and has been gripped groove (41).

4. The apparatus for preparing a collagen scaffold with directional morphology according to claim 2, wherein: the three-dimensional refrigerator is characterized in that three support frames (8) are fixedly mounted at the bottom of an inner cavity of the placing table (2), the shaping cylinder (9) is fixedly mounted at the top of the three support frames (8), and a refrigerating hole (91) is formed in the bottom of the shaping cylinder (9).

5. The apparatus for preparing a collagen scaffold with directional morphology according to claim 2, wherein: sealing mechanism (6) are including sealing spring (61), movable block (62), sealed dish (63) and protection section of thick bamboo (64), the bottom fixedly connected with movable block (62) of sealing spring (61), the external fixed surface of movable block (62) has cup jointed sealed dish (63), the edge fixed mounting at movable block (62) top has protection section of thick bamboo (64), the top and the installation lid (3) fixed connection of protection section of thick bamboo (64), the top of sealing spring (61) and the bottom fixed connection of installation lid (3).

6. The apparatus for preparing a collagen scaffold with directional morphology according to claim 5, wherein: the number of the sealing springs (61) is four, the four sealing springs (61) are distributed in an equidistant circumference mode by taking the circle center of the moving block (62) as the reference, and the protection cylinder (64) is made of nylon and has the thickness value of ten millimeters.

7. The apparatus for preparing a collagen scaffold with directional morphology according to claim 2, wherein: the number of the tightening rings (12) is ten, every five of the tightening rings (12) are divided into two groups, and each group of the tightening rings (12) are concentric rings which are mutually sleeved from outside to inside and gradually decrease in size.

8. The apparatus for preparing a collagen scaffold with directional morphology according to claim 2, wherein: the number of the placing grooves (15) is four, the placing grooves are distributed on the circumference at equal intervals by taking the circle center of the shaping barrel (9) as a reference, and each placing groove (15) is arc-shaped.

9. The apparatus for preparing a collagen scaffold with directional morphology according to claim 2, wherein: the lifting ring (5) is in a ring shape, and the height value of the lifting ring (5) is smaller than the distance value between the bottom of the mounting cover (3) and the bottom of the inner cavity of the placing table (2).

Technical Field

The invention belongs to the technical field of preparation of collagen scaffolds, and particularly relates to a preparation method and preparation equipment of a collagen scaffold with an oriented morphology.

Background

When soft tissues are seriously damaged, the tissue repair and related functional reconstruction are difficult to realize in a short time, so the method is generally adopted for solving the problems, the tissue transplantation faces the rejection phenomenon which is limited in source and possibly occurs, the research surface is provided, the collagen scaffold with the oriented morphology can better support bone marrow mesenchymal stem cells, the extension of collagen fibers in the spreading direction can be promoted, and the broken tissue cells can be repaired.

At present, the preparation of the collagen scaffold with the oriented morphology usually adopts a mode of mold and oriented freezing to freeze and mold the collagen scaffold so as to obtain a finished product; however, the mold for holding the collagen mixed solution is made of rigid material, and is difficult to generate large degree of cold shrinkage deformation when being cooled in the directional freezing process, and the teacher mixing inside the mold is easy to generate large degree of deformation in the process of freezing the liquid into a colloidal form, so that the inner wall of the mold cannot be always attached to the colloidal collagen in the process, and the freezing efficiency is reduced; meanwhile, the sealing performance of the mold is limited by the structure and cannot keep good performance, and the sealing performance is damaged due to cold shrinkage and slight deformation generated by the cold of the mold in the directional freezing process; at the same time, the frozen material for directional freezing needs to fill the entire interior of the apparatus, which causes unnecessary waste.

Disclosure of Invention

The invention aims to provide a preparation method and a preparation device of a collagen scaffold with oriented morphology, so as to solve the problems in the background technology.

In order to achieve the above purpose, the invention provides the following technical scheme: a preparation method of a collagen scaffold with oriented morphology comprises the following steps:

taking the upper spine soft tissue of an animal, extracting collagen, then placing the extracted collagen in a prepared mould, adding a prepared acetic acid solution into the prepared mould, taking pH test paper, and measuring the pH value in the mould to control the pH value to be between 4 and 5;

stirring the collagen solution in the mould by using an experimental bar to mix and form, and then refrigerating for 30 minutes, wherein the refrigerating temperature is set to be 4 ℃;

placing a mould filled with the refrigerated and molded collagen mixed solution in a buffer ring, so that the placing block is clamped in the placing groove, and the outer wall of the mould is in contact with the buffer ring and extrudes the buffer ring;

injecting a frozen substance into the placing table, covering the mounting cover on the placing table, clamping the outer sealing ring on the outer sealing groove, and driving the frozen substance to rise upwards by the lifting ring which extends into the placing table;

the mounting cover moving downwards drives the pressing ring to move downwards and wrap the outer surface of the connecting block to form a sealing effect, and simultaneously drives the sealing mechanism to extrude and move into the die;

at this time, the inside of the placing table was frozen in a directional manner, and after waiting for 40 minutes, the collagen scaffold was molded.

A preparation device of a collagen bracket with directional morphology comprises a protective shell, a placing table and a mounting cover, wherein a sealing mechanism and a pressing ring are fixedly mounted at the bottom of the mounting cover, a shaping cylinder is arranged in the middle of an inner cavity of the placing table, an inner groove is formed in the inner wall of the shaping cylinder, fixing rings and a contraction ring are movably clamped in the inner groove in a staggered mode, a buffer ring is sleeved on the fixing rings and the contraction ring in an inner movable mode, a placing groove is formed in the top of the shaping cylinder, a mold is movably clamped in the placing groove, an outer sealing groove is formed in the top of the placing table, an outer sealing ring is hermetically sleeved on the inner sealing ring of the outer sealing groove, the top of the outer sealing ring is fixedly connected with the mounting cover, a lifting ring is fixedly mounted at the bottom of the mounting cover, when a collagen mixed solution is filled in the mold and enters the shaping cylinder, a reaction force can be applied to the outer wall of the mold from the outer side by the extruded buffer ring, when carrying out directional freezing, the mould receives cold and takes place slight shrink, and at this moment, the shrink ring just can contract and ensnare the cushion ring with solid fixed ring, and the cushion ring just can tightly wrap up the surface of mould, the packing force through the parcel for the inside side of inner wall of mould is tight, thereby reduces the internal diameter value of mould, thereby makes the inner wall of mould laminate its inside collagen mixed liquid that holds more, and then improves the refrigeration efficiency of collagen mixed liquid.

Preferably, the protective housing is made of a heat insulating material and is specifically a high silicon-oxygen cotton, the placing table is movably sleeved inside the protective housing, the sealing mechanism is fixedly installed in the middle of the bottom surface of the mounting cover, the bottom of the sealing mechanism extends into the mold, the placing block is movably clamped inside the placing groove, the top of the placing block is fixedly installed with a connecting block, the inner side surface of the bottom of the connecting block is fixedly connected with the top of the mold, handles are fixedly installed on the left side and the right side of the mounting cover, a holding groove is formed in the bottom of each handle, the outer sealing ring is made of a rubber block, after the mounting cover is mounted, the outer sealing ring is movably clamped in the outer sealing groove, so that the sealing of the inner space of the placing table is ensured, the energy dissipation of frozen substances in the placing table is avoided, and meanwhile, the pressing ring is movably clamped at the top of the shaping cylinder along with the downward movement of the mounting cover, the surface at the connecting block will be wrapped up at this moment to guaranteed the inside leakproofness of mould, and place the inside of piece activity joint and provide the supporting role for the mould at the standing groove, make the mould can place the inside at a design section of thick bamboo steadily, owing to place the inside and outside difference in temperature of platform big, make the installation lid receive from last pressure down, thereby strengthened the pressing force degree of pressing the ring to design bobbin top portion, further strengthened the sealed effect of mould.

Preferably, the bottom of the inner cavity of the placing table is fixedly provided with three support frames, the shaping cylinder is fixedly arranged at the tops of the three support frames, the bottom of the shaping cylinder is provided with a refrigerating hole, the three support frames are used for supporting the shaping cylinder, on one hand, frozen substances injected into the placing table flow through the bottom of the shaping cylinder and increase the cold area, so that the freezing efficiency is accelerated, meanwhile, the contact area between the shaping cylinder and the frozen substances in the placing table is larger due to the arrangement of the refrigerating hole, the directional freezing effect is better, the forming speed of the collagen support is accelerated, when the lifting ring completely enters the inside of the placing table, the frozen substances with the same volume can be discharged, the liquid level of the frozen substances in the placing table is caused to rise, and the outer surface of the shaping cylinder can be cooled by hands to the maximum degree.

Preferably, the sealing mechanism comprises a sealing spring, a moving block, a sealing disc and a protective cylinder, the bottom end of the sealing spring is fixedly connected with the moving block, the sealing disc is fixedly sleeved on the outer surface of the moving block, the protective cylinder is fixedly installed at the edge of the top of the moving block, the top of the protective cylinder is fixedly connected with the installation cover, the top end of the sealing spring is fixedly connected with the bottom of the installation cover, when the installation cover is installed on the placing table, the sealing spring is driven to move downwards, then the moving block and the sealing disc are driven to move downwards, so that the outer surface of the sealing disc is in contact with the inner wall of the mold and is extruded, the sealing performance of an opening at the top of the mold is maintained, the sealing spring is extruded at the moment, after the installation cover is completely installed in place, the sealing spring, the moving block, the sealing disc and the protective cylinder completely enter the interior of the mold, and the sealing spring generates a reaction force after being compressed, and then promote the movable block downwards for the movable block drives sealed dish and moves downwards, has strengthened sealed effect on the one hand, has also kept the stability of installation lid after the installation simultaneously.

Preferably, the quantity of sealing spring is four, four sealing spring uses the centre of a circle of movable block as the benchmark and is equidistant circumference distribution, protection section of thick bamboo is made by nylon and the thickness value is ten millimeters, four sealing spring distribute with mutually perpendicular's mode for the top atress of movable block is more balanced, and sealing spring's quantity has then changed pressure into more lasting, the more balanced spring reaction force of atress, protection section of thick bamboo then can extrude through deformation when the distance of movable block and installation lid shortens and carry out the adaptation, thereby for sealing spring provides safeguard function, and the nylon material has low temperature resistant characteristic, not only the protecting effect is good, and economical and practical, accord with the low-cost economic principle of modern enterprise.

Preferably, the straining ring is made by memory alloy and quantity is ten, ten the straining ring divide into two sets for every five as a set, every group the straining ring is for cup jointing each other and size degressive concentric ring in proper order from outside to inside, the straining ring is made by memory alloy, has quick deformation and quick recovery's characteristic, when the frozen material in placing the platform acts on, can refrigerate the straining ring, and the straining ring receives the cold back, can inwards contract, because every group straining ring is that five inside and outside diameters are different concentric ring are constituteed, consequently, the contractile force that produces after the shrinkage will multiply, thereby from outside strangle the buffering ring, make the buffering ring exert pressure with the mode of buffering to the surface of mould, and then reduce the internal diameter value of mould by force, make the inner wall of mould more laminate and become the collagen support of colloid, and then promote refrigeration efficiency.

Preferably, the quantity of standing groove is four and is equidistant circumference distribution with the centre of a circle of a shaping section of thick bamboo as the benchmark, every the shape of standing groove is the arc, and the standing groove is used for the joint to place and places the piece for with place the connecting block that the piece links to each other and mould and obtain a stable support, when placing the inside of piece activity joint at the standing groove, install the installation lid of placing the bench face and can exert pressure downwards, drive the connecting block and will place the piece and firmly suppress the inside at the standing groove, thereby keep the stability of mould when carrying out directional freezing.

Preferably, the shape of lifting ring is ring shape, the height value of lifting ring is less than the distance value between the bottom of installation lid and the bottom of placing the platform inner chamber, lifting ring is used for increasing the dead weight of installation lid on the one hand, make the installation lid install more stable after placing the platform, the outer seal ring can connect in the inside of outer seal groove more compactly, not only make the installation lid install more stable, but also strengthened the sealed effect of placing the platform simultaneously, and lifting ring when getting into the inside of placing the platform, can occupy the space of placing the platform inside, when the inside of placing the platform pours into frozen material into, lifting ring can arrange the frozen material of equal volume into, make the page of frozen material in placing the platform rise, and then reduce the volume of the frozen material of actual need, the use amount of frozen material has been reduced, and the cost is reduced.

The invention has the following beneficial effects:

1. the efficient directional freezing forming process is realized by arranging the shaping cylinder, the cooling hole, the inner groove, the fixing ring, the contraction ring, the buffer ring, the mold and the like, the shaping cylinder conducts heat, the shaping cylinder is rapidly cooled, the mold is rapidly cooled, the surface area of the shaping cylinder is increased by arranging the cooling hole, the cooling speed of the mold is further increased, the buffer ring is inwards pulled in a cold shrinkage mode after being cooled by arranging the fixing ring and the contraction ring, the mold is compressed by the buffer ring, the compressed mold can integrally contract inwards, meanwhile, the inner wall of the mold is sunken inwards, and the mold is further attached to a collagen support colloid in the mold, and the freezing efficiency of the collagen colloid is higher.

2. The invention realizes the sealing function of the die by arranging the sealing mechanism, the mounting cover, the pressing ring, the placing block, the connecting block, the placing block, the sealing mechanism and the like, the hand-pressed mounting cover with the lifting ring for mounting the mounting cover is arranged, so that the pressing ring is driven to be pressed and wrapped on the outer edge of the top of the fixed cylinder, meanwhile, the placing block is clamped in the placing groove, the pressing ring and the connecting block are matched to form a first sealing defense line on the outer side of the die, the moving block and the sealing disc are driven to move downwards by the sealing spring and enter the die, the outer surface of the sealing disc is in contact with the inner wall of the die, and the sealing performance of the opening on the top of the die is maintained by the deformation of the sealing disc.

3. Accomplish the installation inside the back of support frame with the outer sealing ring joint of its bottom at the installation lid, at this moment, promote the ring and enter into the inside of placing the platform completely, and the inside of placing the platform has injected into frozen material, and the entering is placed the inside frozen material that can arrange the same volume of promotion ring of platform, make the liquid level of frozen material rise, thereby cross the surface of a shaping section of thick bamboo, and then make a shaping section of thick bamboo and mould continuously catch a cold, and with the freezing shaping of collagen support in the mould, and the joining of promotion ring makes the injection volume of frozen material reduce, thereby reduce the consumption of frozen material, and the cost is reduced.

Drawings

FIG. 1 is a front cut-away schematic view of the structure of the present invention;

FIG. 2 is a schematic view of the interior of the standing board according to the present invention;

FIG. 3 is a schematic front view of the structure of the present invention;

FIG. 4 is a schematic bottom view of the structure of the present invention;

FIG. 5 is a schematic diagram of the separation of the mounting cap, lifting ring, sealing mechanism and outer sealing ring of the present invention;

FIG. 6 is a schematic structural view of the mold of the present invention;

FIG. 7 is a schematic view of the separation of the setting cylinder, the fixing ring, the tightening ring, the cushion ring and the placement groove of the present invention;

FIG. 8 is a bottom view of the sizing cylinder of the present invention;

FIG. 9 is an enlarged view of the structure at A in FIG. 1 according to the present invention

In the figure: 1. a protective shell; 2. a placing table; 3. installing a cover; 4. a carrying handle; 41. a holding groove; 5. a lifting ring; 6. a sealing mechanism; 61. a seal spring; 62. a moving block; 63. sealing the disc; 64. a protective cylinder; 7. pressing a ring; 8. a support frame; 9. shaping the cylinder; 91. a refrigeration hole; 10. an inner tank; 11. a fixing ring; 12. a shrink ring; 13. a buffer ring; 14. a mold; 15. a placement groove; 16. placing the blocks; 17. connecting blocks; 18. an outer seal groove; 19. and an outer seal ring.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1 to 9, in an embodiment of the present invention, a method for preparing a collagen scaffold with oriented morphology includes the following steps:

taking the upper spine soft tissue of an animal, extracting collagen, then placing the extracted collagen in a prepared mould, adding a prepared acetic acid solution into the prepared mould, taking pH test paper, and measuring the pH value in the mould to control the pH value to be between 4 and 5;

stirring the collagen solution in the mould by using an experimental bar to mix and form, and then refrigerating for 30 minutes, wherein the refrigerating temperature is set to be 4 ℃;

placing a mould filled with the refrigerated and molded collagen mixed solution in a buffer ring, so that the placing block is clamped in the placing groove, and the outer wall of the mould is in contact with the buffer ring and extrudes the buffer ring;

injecting a frozen substance into the placing table, covering the mounting cover on the placing table, clamping the outer sealing ring on the outer sealing groove, and driving the frozen substance to rise upwards by the lifting ring which extends into the placing table;

the mounting cover moving downwards drives the pressing ring to move downwards and wrap the outer surface of the connecting block to form a sealing effect, and simultaneously drives the sealing mechanism to extrude and move into the die;

at this time, the inside of the placing table was frozen in a directional manner, and after waiting for 40 minutes, the collagen scaffold was molded.

A preparation device of a collagen bracket with directional morphology comprises a protective shell 1, a placing table 2 and an installing cover 3, wherein the bottom of the installing cover 3 is fixedly provided with a sealing mechanism 6 and a pressing ring 7, the middle part of the inner cavity of the placing table 2 is provided with a shaping cylinder 9, the inner wall of the shaping cylinder 9 is provided with an inner groove 10, the inner part of the inner groove 10 is movably clamped with a fixing ring 11 and a contraction ring 12 in a staggered mode, the inner parts of the fixing ring 11 and the contraction ring 12 are movably sleeved with a buffer ring 13, the top of the shaping cylinder 9 is provided with a placing groove 15, the inner part of the placing groove 15 is movably clamped with a mould 14, the top of the placing table 2 is provided with an outer sealing groove 18, the inner part of the outer sealing groove 18 is sleeved with an outer sealing ring 19, the top of the outer sealing ring 19 is fixedly connected with the installing cover 3, the bottom of the installing cover 3 is fixedly provided with a lifting ring 5, after the mould 14 is filled with a collagen mixed solution and enters the inside of the shaping cylinder 9, the extruded buffer ring 13 exerts a reaction force from the outside to the outer wall of the mold 14, when directional freezing is performed, the mold 14 is cooled and slightly shrinks, at the moment, the compression ring 12 and the fixing ring 11 shrink and tighten the buffer ring 13, the buffer ring 13 tightly wraps the outer surface of the mold 14, the inner wall of the mold 14 is pressed inwards through the wrapping pressing force, the inner diameter value of the mold 14 is reduced, the inner wall of the mold 14 is more attached to the collagen mixed liquid contained in the mold, and the freezing efficiency of the collagen mixed liquid is improved.

Wherein, the protective shell 1 is made of heat insulating material and is specifically a high silicon cotton, the placing platform 2 is movably sleeved in the protective shell 1, the sealing mechanism 6 is fixedly installed in the middle of the bottom surface of the installing cover 3, the bottom of the sealing mechanism 6 extends into the mold 14, the placing block 16 is movably clamped in the placing groove 15, the top of the placing block 16 is fixedly installed with a connecting block 17, the inner side surface of the bottom of the connecting block 17 is fixedly connected with the top of the mold 14, the left side and the right side of the installing cover 3 are both fixedly installed with the carrying handle 4, the bottom of the carrying handle 4 is provided with a holding groove 41, the outer sealing ring 19 is made of rubber blocks, after the installing cover 3 is installed, the outer sealing ring 19 is movably clamped in the outer sealing groove 18, thereby ensuring the sealing of the inner space of the placing platform 2, avoiding the energy dissipation of frozen substances in the placing platform 2, and simultaneously, the pressing ring 7 is movably clamped at the top of the shaping cylinder 9 along with the downward movement of the installing cover 3, will wrap up at connecting block 17's surface this moment, thereby guaranteed the inside leakproofness of mould 14, and place 16 movable joint and provide the supporting role at the inside of standing groove 15 for mould 14, make mould 14 can place the inside at design section of thick bamboo 9 steadily, moreover because it is big to place the inside and outside difference in temperature of platform 2, make installation lid 3 receive from last pressure down, thereby increased the pressing force degree of pressing ring 7 to design section of thick bamboo 9 top, further strengthened the sealed effect of mould 14.

Wherein, the bottom fixed mounting who places the 2 inner chambers of platform has three support frame 8, a design section of thick bamboo 9 fixed mounting is at the top of three support frame 8, refrigeration hole 91 has been seted up to the bottom of a design section of thick bamboo 9, three support frame 8 is used for realizing the support to a design section of thick bamboo 9, on the one hand, make the freezing material that pours into in placing platform 2 flow through and increase cold area from the bottom of a design section of thick bamboo 9, make freezing efficiency accelerate, the seting up of refrigeration hole 91 makes a design section of thick bamboo 9 bigger with the area of contact of placing the freezing material in platform 2 simultaneously, make directional freezing effect better, thereby accelerate the shaping speed of collagen support, when promotion ring 5 gets into the inside of placing platform 2 completely, can arrange the freezing material of equal volume, cause the liquid level of placing the freezing material in platform 2 to rise, make the outer surface of a design section of thick bamboo 9 can the biggest hand cold.

Wherein, the sealing mechanism 6 comprises a sealing spring 61, a moving block 62, a sealing disc 63 and a protective cylinder 64, the bottom end of the sealing spring 61 is fixedly connected with the moving block 62, the outer surface of the moving block 62 is fixedly sleeved with the sealing disc 63, the edge of the top of the moving block 62 is fixedly provided with the protective cylinder 64, the top of the protective cylinder 64 is fixedly connected with the mounting cover 3, the top end of the sealing spring 61 is fixedly connected with the bottom of the mounting cover 3, when the mounting cover 3 is mounted on the placing table 2, the sealing spring 61 is driven to move downwards, then the moving block 62 and the sealing disc 63 are driven to move downwards, so that the outer surface of the sealing disc 63 is contacted with the inner wall of the mold 14 and is extruded, thereby the sealing performance of the top opening of the mold 14 is maintained, at this time, the sealing spring 61 is extruded, after the mounting cover 3 is completely mounted in place, the sealing spring 61, the moving block 62, the sealing disc 63 and the protective cylinder 64 completely enter the interior of the mold 14, the sealing spring 61 can generate a reaction force after being compressed, and then the moving block 62 is pushed downwards, so that the moving block 62 drives the sealing disc 63 to move downwards, on one hand, the sealing effect is enhanced, and meanwhile, the stability of the mounting cover 3 after mounting is also kept.

Wherein, sealing spring 61's quantity is four, four sealing spring 61 are equidistant circumference distribution with the centre of a circle of movable block 62 as the benchmark, protection section of thick bamboo 64 is made by nylon and the thickness value is ten millimeters, four sealing spring 61 distribute with mutually perpendicular's mode, make the top atress of movable block 62 more balanced, and sealing spring 61's quantity has then changed pressure into more lasting, the more balanced spring reaction force of atress, protection section of thick bamboo 64 then can extrude through deformation when the distance of movable block 62 and installation lid 3 shortens and carry out the adaptation, thereby for sealing spring 61 provides safeguard function, and the nylon material has low temperature resistant characteristic, not only the protecting effect is good, and economical and practical, accord with the low-cost economic principle of modern enterprise.

Wherein, the number of the tightening rings 12 is ten, every five of the tightening rings 12 are divided into two groups, each group of the tightening rings 12 is concentric rings which are mutually sleeved from outside to inside and the sizes of the tightening rings are gradually reduced, the tightening rings 12 are made of memory alloy, and the device has the characteristics of quick deformation and quick recovery, when the frozen substance in the placing table 2 acts, the contraction ring 12 is cooled, and after the contraction ring 12 is cooled, it contracts inward, because each group of the shrink rings 12 consists of five concentric rings with different inner and outer diameters, the shrinkage force generated after cold contraction is multiplied, thereby pinching the cushion ring 13 from the outside, so that the cushion ring 13 applies pressure to the outer surface of the mold 14 in a cushioning manner, and then diminish the internal diameter value of mould 14 by force for the inner wall of mould 14 more laminates and becomes the collagen support of colloid, and then promotes freezing efficiency.

Wherein, standing groove 15's quantity is four and is equidistant circumference distribution with the centre of a circle of a design section of thick bamboo 9 as the benchmark, every standing groove 15's shape is the arc, standing groove 15 is used for the joint to place and places piece 16, make and place connecting block 17 and the mould 14 that piece 16 links to each other and obtain a stable support, when placing 16 movable joints of piece in standing groove 15's inside, install and to apply pressure downwards at the installation lid 3 of placing 2 tops, drive connecting block 17 and will place piece 16 and suppress firmly in standing groove 15's inside, thereby keep the stability of mould 14 when carrying out directional freezing.

Wherein, the lifting ring 5 is in a ring shape, the height value of the lifting ring 5 is smaller than the distance value between the bottom of the mounting cover 3 and the bottom of the inner cavity of the placing platform 2, on one hand, the lifting ring 5 is used for increasing the dead weight of the mounting cover 3, the mounting cover 3 is more stable after being mounted on the placing table 2, the outer sealing ring 19 can be clamped in the outer sealing groove 18 more tightly, the mounting cover 3 is more stable, the sealing effect of the placing table 2 is enhanced, when the lifting ring 5 enters the placing table 2, the lifting ring occupies the space inside the placing table 2, when the freezing substance is injected into the placing table 2, the lifting ring 5 can discharge the freezing substance with the same volume, so that the page of the freezing substance in the placing table 2 is raised, further, the amount of the freezing substances needed actually is reduced, the using amount of the freezing substances is reduced, and the cost is reduced.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.