阅读说明：本技术 一种阿维菌素组合物、发酵工艺及其制备方法 (Abamectin composition, fermentation process and preparation method thereof ) 是由 刘丽虹 刘进峰 于 2021-09-28 设计创作，主要内容包括：本发明提供一种阿维菌素组合物、发酵工艺及其制备方法,包含阿维菌素、羟基聚环氧乙烷嵌段共聚物、分散剂、消泡剂、增稠剂、抑菌剂和水。本发明通过助剂的选择和砂磨工艺、设备的选择,可以制成稳定的悬浮剂；通过新型菌丝发酵工艺,先制作均匀,其有效成分含量稳定；本发明制备得到的悬浮剂可以保持长久的稳定,待发生部分沉淀时,可以快速的恢复到较好的悬浮状态。(The invention provides an abamectin composition, a fermentation process and a preparation method thereof. The stable suspending agent can be prepared by selecting the auxiliary agent and the sand grinding process and equipment; the novel hypha fermentation process is adopted, the preparation is uniform, and the content of active ingredients is stable; the suspending agent prepared by the invention can keep long-term stability, and can quickly recover to a better suspension state when partial precipitation is generated.)

1. An avermectin composition, characterized in that it comprises:

2. an avermectin composition as claimed in claim 1, wherein the hydroxypolyethylene oxide block copolymer has an EO/PO ratio of 1: 1.71.

3. an avermectin composition as claimed in claim 1, wherein the hydroxy polyethylene oxide block copolymer has a hydroxyl number of from 18 to 20mg KOH/g.

4. The avermectin composition of claim 1, wherein the dispersant has an HLB value of from 16.5 to 17.

5. The avermectin composition of claim 1, wherein the dispersing agent further comprises a polymeric polycarboxylate.

6. The avermectin composition of claim 1, wherein the thickener is lithium magnesium silicate.

7. The abamectin composition of claim 1, wherein the bacteriostatic agent is selected from the group consisting of dodecylbenzyl ammonium chloride, isothiazolinone, chlorine dioxide, eugenol, benzoic acid, sodium benzoate, and combinations thereof.

8. An abamectin formulation characterised in that it is prepared from a composition according to any one of claims 1 to 7.

9. The abamectin formulation of claim 8, in the form of a suspension.

10. The avermectin preparation as set forth in claim 8, characterized in that the preparation process is as follows:

s01 raw material mixing

Adding corn starch, soybean cake powder, yeast powder, a defoaming agent, caustic soda flakes, cobalt chloride, sodium molybdate, manganese sulfate, amylase and water into a feeding pool, and stirring until the mixture is milky;

s02 fermentation

Pumping the milky mixture obtained in the step S01 into a fermentation tank, pressing actinomycetes into the fermentation tank from a distributor, adjusting the pH value in the fermentation tank to 6.8, introducing steam, maintaining the pressure, continuously sterilizing for 0.5h, cooling to 28.5 ℃, introducing compressed air into the fermentation tank, operating for 540h, sampling, inspecting and discharging to the fermentation tank after the sampling is qualified;

preparation of S03 preparation

And (4) adding the rest components into the fermentation liquor obtained in the step (S02), crushing, and steaming and removing the residual components.

Technical Field

The invention belongs to the field of pesticides, and particularly relates to an abamectin composition, a fermentation process and a preparation method thereof.

Background

The abamectin is fermented by a strain to obtain hypha, and then is extracted by a solvent to obtain a raw drug with higher content, and then is processed into a preparation. Such an operation brings about many problems. 1, the use of a large amount of solvent brings about many problems of environment and life safety even if the solvent can be recycled. 2, solvent extraction or other extraction followed by further processing of the formulation, which brings about a lot of costs. Therefore, the invention is based on the characteristics of hypha and can be processed into the suspending agent. Saves the processing cost, reduces the use of solvents, ensures the environmental protection and the life safety, and has excellent effect of field experiments.

The pesticide raw materials are prepared into different formulations according to different characteristics, such as more than ten formulations, such as missible oil, suspending agent, wettable powder, granules, water agent, poison bait, mother liquor, mother powder and the like. The suspending agent is a preparation which is prepared by uniformly dispersing the solid pesticide raw material in water by particles with the particle size of less than 4 microns, and the international code is SC. Because SC does not have the problem of dust flying like Wet Powder (WP), is nonflammable and explosive, has small particle size, high biological activity, larger specific gravity and smaller packaging volume, and is safe and environment-friendly compared with other pesticide formulations, SC becomes a pesticide variety with larger tonnage in a novel water-based pesticide formulation.

Disclosure of Invention

The invention provides an abamectin composition, which comprises the following components in part by weight:

as an embodiment of the present invention, the hydroxypolyethylene oxide block copolymer has an EO/PO ratio of 1: 1.71.

in one embodiment of the present invention, the hydroxyl value of the hydroxypolyethylene oxide block copolymer is 18 to 20mg KOH/g.

In one embodiment of the present invention, the HLB value of the dispersant is 16.5 to 17.

As an embodiment of the present invention, the dispersant further comprises a polymeric polycarboxylate.

In one embodiment of the present invention, the thickener is lithium magnesium silicate.

As an embodiment of the invention, the bacteriostatic agent is selected from one or two of dodecyl benzyl ammonium chloride, isothiazolinone, chlorine dioxide, sodium metachlor, benzoic acid and sodium benzoate.

An abamectin preparation is prepared from the abamectin composition.

As an embodiment of the present invention, the formulation is in the form of a suspension.

As an embodiment of the invention, the preparation process of the abamectin comprises the following steps:

s01 raw material mixing

Adding corn starch, soybean cake powder, yeast powder, a defoaming agent, caustic soda flakes, cobalt chloride, sodium molybdate, manganese sulfate, amylase and water into a feeding pool, and stirring until the mixture is milky;

s02 fermentation

Pumping the milky mixture obtained in the step S01 into a fermentation tank, pressing actinomycetes into the fermentation tank from a distributor, adjusting the pH value in the fermentation tank to 6.8, introducing steam, maintaining the pressure, continuously sterilizing for 0.5h, cooling to 28.5 ℃, introducing compressed air into the fermentation tank, operating for 540h, sampling, inspecting and discharging to the fermentation tank after the sampling is qualified;

preparation of S03 preparation

And (4) adding the rest components into the fermentation liquor obtained in the step (S02), crushing, and steaming and removing the residual components.

Has the advantages that:

1. the stable suspending agent can be prepared by selecting the auxiliary agent and the sand grinding process and equipment;

2. the novel hypha fermentation process is adopted, the preparation is uniform, and the content of active ingredients is stable;

3. the suspending agent prepared by the invention can keep long-term stability, and can quickly recover to a better suspension state when partial precipitation is generated.

Detailed Description

Abamectin, english name Abamaectin, accession number: 65195-55-3 and 65195-56-4; it is a macrolide compound with insecticidal, acaricidal and nematicidal activities.

The abamectin has stomach toxicity and contact killing effect on mites and insects, and can not kill eggs. The action mechanism is different from that of the common pesticide in that the action mechanism interferes with the neurophysiological activity and stimulates the release of gamma-aminobutyric acid, and the aminobutyric acid has an inhibition effect on the nerve conduction of arthropods. After the mite adults, nymphs and insect larvae are contacted with the abamectin, paralysis symptoms appear, and the mites die after 2-4 days without moving or taking food. Because the quick dehydration of the insects is not caused, the lethal effect of the abamectin is slower. The abamectin has direct contact killing effect on predatory insects and parasitic natural enemies, but has little damage to beneficial insects because of little residue on the surface of the plant. The abamectin is adsorbed by soil in soil and cannot move and is decomposed by microorganisms, so that the abamectin has no accumulation effect in the environment and can be used as a component of comprehensive control. The preparation is easy to prepare, can be used after being poured into water and slightly stirred, and is safe to crops.

The abamectin is fermented by a strain to obtain hypha, and then is extracted by a solvent to obtain a raw drug with higher content, and then is processed into a preparation. Such an operation brings about many problems. 1, the use of a large amount of solvent brings about many problems of environment and life safety even if the solvent can be recycled. 2, solvent extraction or other extraction followed by further processing of the formulation, which brings about a lot of costs.

The invention provides a novel avermectin fermentation method and a preparation process, which solve the problems and comprise the following steps:

s01 raw material mixing

Adding corn starch, soybean cake powder, yeast powder, a defoaming agent, caustic soda flakes, cobalt chloride, sodium molybdate, manganese sulfate, amylase and water into a feeding pool, and stirring until the mixture is milky;

s02 fermentation

Pumping the milky mixture obtained in the step S01 into a fermentation tank, pressing actinomycetes into the fermentation tank from a distributor, adjusting the pH value in the fermentation tank to 6.8, introducing steam, maintaining the pressure, continuously sterilizing for 0.5h, cooling to 28.5 ℃, introducing compressed air into the fermentation tank, operating for 540h, sampling, inspecting and discharging to the fermentation tank after the sampling is qualified;

preparation of S03 preparation

And (4) adding the rest components into the fermentation liquor obtained in the step (S02), crushing, and steaming and removing the residual components.

The specific detailed preparation method is as follows:

s01 raw material mixing

15.5t of corn starch, 2.5t of soybean cake powder, 950kg of yeast powder, 600kg of defoaming agent, 25kg of caustic soda flakes, 1.5kg of cobalt chloride, 2kg of sodium molybdate, 500g of manganese sulfate, 3.5kg of amylase and 40m of water are put into a feeding pool³Stirring to be milky;

s02 fermentation

Pumping actinomycetes into a fermentation tank by using a feeding pump, pressing actinomycetes to the fermentation tank from a distributor, adjusting the pH value in the fermentation tank to 6.8, opening front and rear valves of a steam filter to introduce steam, adjusting the opening of a valve to enable a pressure gauge on a precision filter to display 0.13MPa, then directly introducing steam of 0.4MPa and 150 ℃ into the fermentation tank, maintaining the pressure and continuously sterilizing for 0.5h when the temperature on the fermentation tank displays 120 ℃ and the pressure gauge displays 0.13MPa, then introducing circulating water into an inner coil and an outer coil of the fermentation tank to enable the temperature in the fermentation tank to reach 28.5 ℃, introducing compressed air into the fermentation tank, operating for 540h, sampling, inspecting and discharging to the fermentation tank after the temperature is qualified;

preparation of S03 preparation

Adding the rest components into the fermentation liquor, then crushing, and adopting a double-effect falling film evaporator to remove the excess moisture from the crushed material according to the content requirement.

Hydroxy polyethylene oxide block copolymers

In the present invention, the EO/PO ratio of the hydroxypolyethylene oxide block copolymer is 1: 1.71; in particular to the brand of Ethylan NS-500 LQ.

As an embodiment of the present invention, the hydroxyl value of the hydroxypolyethylene oxide block copolymer is 18 to 20mg KOH/g; the specific brand is 1603# of Nantong de-Yi chemical industry Co.

In one embodiment of the present invention, the hydroxypolyethylene oxide block copolymer is a hydroxypolyethylene oxide block copolymer of a brand number of ethyllan NS-500LQ and a hydroxypolyethylene oxide block copolymer of a brand number of 1603# in a mass ratio of 7: 2, or a mixture thereof.

Dispersing agent

As the name suggests, the dispersant acts as a dispersing agent. A Dispersant (Dispersant) is a surfactant that has both lipophilic and hydrophilic properties in opposite directions within a molecule. The amphiphilic agent is capable of uniformly dispersing solid and liquid particles of inorganic and organic pigments which are difficult to dissolve in liquids, and also preventing settling and agglomeration of the particles to form stable suspensions.

Common dispersants for pesticides are surfactants whose function is to keep the particles dispersed and prevent agglomeration. As the alkyl aryl sulfonate, a formaldehyde condensate thereof, a lignosulfonate, an alkylphenol polyoxyethylene ether formaldehyde condensate, a sulfate, etc. are usually used.

In one embodiment of the invention, the dispersant is selected from dispersants having an HLB value of 16.5 to 17, more preferably a dispersant having an HLB value of 16.9, and more particularly Wacker Germany VOK-G5000.

In one embodiment of the invention, the dispersant further comprises a polymeric polycarboxylate, specifically a GY-D series carboxylate dispersant of Beijing Guangyuan agricultural chemical Co., Ltd, and specifically GY-D800 of Beijing Guangyuan agricultural chemical Co., Ltd.

As an embodiment of the present invention, the dispersant further comprises calatlox 4913.

As an embodiment of the invention, the dispersant is prepared from German Wolk VOK-G5000, Guangyuan Yinong GY-D800 and Daatlox 4913 according to the weight ratio of 1: 1: 2.

Defoaming agent

Common defoamers can reduce the surface tension of water, solutions, suspensions, etc., prevent foam formation, or reduce or eliminate the original foam.

The defoamer should have the following properties: the defoaming power is strong, and the dosage is less; adding the product into a foaming system does not influence the basic properties of the system, namely does not react with a system to be defoamed; surface tension is small; fourthly, the balance between the surface and the surface is good; the heat resistance is good; sixthly, the diffusivity and the permeability are good, and the positive spreading coefficient is higher; the chemical stability and the oxidation resistance are strong; the gas has good solubility and permeability; ninthly, the dissolubility in the foaming solution is small; the red (R) has no physiological activity and high safety.

The agricultural chemicals include herbicide, insecticide, bactericide, etc., generally are complex organic matters, and in order to make them uniformly distributed on crops, surfactant must be added to make them into wettable powder or oil-in-water emulsion. Upon dilution, a foam will be produced. The flotation of the foam reduces the uniformity of the pesticide water dispersion, influences the pesticide effect and simultaneously disturbs the spraying operation.

In the invention, the defoaming agent is an organic silicon defoaming agent, and the specific mark is Mi image SAG 672.

In the invention, the specific use method of the defoaming agent is that the organic silicon defoaming agent is firstly mixed with deionized water, and the weight ratio of the two is 10: 1, and then mixed into the whole system.

Thickening agent

The thickener is also called gelatinizer, is a substance capable of increasing the viscosity of latex and liquid, and is also called paste when being used for food. The thickening agent can improve the viscosity of the system, so that the system is kept in a uniform and stable suspension state or an emulsion state, or forms gel; most thickeners combine an emulsifying action. Can be divided into two main categories of natural and synthetic. The natural products are mostly prepared from plants and seaweed containing polysaccharide viscous substances, such as starch, acacia, pectin, agar, gelatin, seaweed gel, carrageenan, dextrin, etc., and general gelatin, soluble starch, polysaccharide derivatives, etc. can be used in cosmetics; the synthetic product includes carboxymethyl cellulose, propylene glycol alginate, methyl cellulose, sodium starch phosphate, sodium carboxymethyl cellulose, sodium alginate, casein, sodium polyacrylate, polyoxyethylene, and polyvinylpyrrolidone.

In one embodiment of the present invention, the thickener is lithium magnesium silicate.

Bacteriostatic agent

Bacteriostatic agents are substances that inhibit the growth of bacteria. The bacteriostatic agent may not kill bacteria, but it can inhibit the growth of bacteria, prevent the bacteria from breeding too much and endanger health.

As an embodiment of the invention, the bacteriostatic agent is selected from one or two of dodecyl dimethyl benzyl ammonium chloride, isothiazolinone, chlorine dioxide, sodium perchlorate, benzoic acid and sodium benzoate.

In a preferred mode of the invention, the bacteriostatic agent is isothiazolinone.

The invention is further described with reference to the following examples:

example 1

This example provides an avermectin composition comprising:

the embodiment provides an avermectin suspending agent, and the preparation process of the suspending agent is as follows:

s01 raw material mixing

15.5t of corn starch, 2.5t of soybean cake powder, 950kg of yeast powder, 600kg of defoaming agent, 25kg of caustic soda flakes, 1.5kg of cobalt chloride, 2kg of sodium molybdate, 500g of manganese sulfate, 3.5kg of amylase and 40m of water are put into a feeding pool³Stirring to be milky;

s02 fermentation

Pumping into a fermentation tank by using a feeding pump, pressing 8000 units of actinomycetes to the fermentation tank from a distributor, adjusting the pH value in the fermentation tank to 6.8, opening front and rear valves of a steam filter to introduce steam, adjusting the opening of a valve to enable a pressure gauge on a precision filter to display 0.13MPa, then directly introducing steam with 0.4MPa and 150 ℃ into the fermentation tank, maintaining the pressure and continuously sterilizing for 0.5h when the temperature on the fermentation tank displays 120 ℃ and the pressure gauge displays 0.13MPa, then introducing circulating water into an inner coil and an outer coil of the fermentation tank to enable the temperature in the fermentation tank to reach 28.5 ℃, introducing compressed air into the fermentation tank, and discharging to the fermentation tank after sampling and checking are qualified after running for 540 h;

preparation of S03 preparation

Adding the rest components into the fermentation liquor, then crushing, and using a double-effect falling film evaporator to remove the excess moisture from the crushed material according to the requirement of 1% of the abamectin content.

In the invention, an ultrasonic nano crusher is used for crushing treatment, and the type of the ultrasonic nano crusher is JY-3500.

In this example, the hydroxypolyethylene oxide block copolymer is a hydroxypolyethylene oxide block copolymer of a brand number of ethyl lan NS-500LQ and a hydroxypolyethylene oxide block copolymer of a brand number of 1603# in a mass ratio of 7: 2, or a mixture thereof.

In this example, the dispersant was prepared from German Wolk VOK-G5000, Guangyuan Yinong GY-D800, and Daatlox 4913 in the proportions of 1: 1: 2.

In the embodiment, the defoaming agent is an organic silicon defoaming agent, and the specific mark is Mi image SAG 672; the specific use method of the defoaming agent is that the organic silicon defoaming agent is mixed with deionized water firstly, and the weight ratio of the two is 10: 1, and then mixed into the whole system.

In this embodiment, the thickener is lithium magnesium silicate.

In this embodiment, the bacteriostatic agent is isothiazolinone.

Example 2

This example provides an avermectin composition comprising:

the embodiment provides an avermectin suspending agent, and the preparation process of the suspending agent is as follows:

s01 raw material mixing

15.5t of corn starch, 2.5t of soybean cake powder, 950kg of yeast powder, 600kg of defoaming agent, 25kg of caustic soda flakes, 1.5kg of cobalt chloride, 2kg of sodium molybdate, 500g of manganese sulfate, 3.5kg of amylase and 40m of water are put into a feeding pool³Stirring to be milky;

s02 fermentation

Pumping into a fermentation tank by using a feeding pump, pressing 8000 units of actinomycetes to the fermentation tank from a distributor, adjusting the pH value in the fermentation tank to 6.8, opening front and rear valves of a steam filter to introduce steam, adjusting the opening of a valve to enable a pressure gauge on a precision filter to display 0.13MPa, then directly introducing steam with 0.4MPa and 150 ℃ into the fermentation tank, maintaining the pressure and continuously sterilizing for 0.5h when the temperature on the fermentation tank displays 120 ℃ and the pressure gauge displays 0.13MPa, then introducing circulating water into an inner coil and an outer coil of the fermentation tank to enable the temperature in the fermentation tank to reach 28.5 ℃, introducing compressed air into the fermentation tank, and discharging to the fermentation tank after sampling and checking are qualified after running for 540 h;

preparation of S03 preparation

Adding the rest components into the fermentation liquor, then crushing, and using a double-effect falling film evaporator to remove the excess moisture from the crushed material according to the requirement of 1% of the abamectin content.

In the invention, an ultrasonic nano crusher is used for crushing treatment, and the type of the ultrasonic nano crusher is JY-3500.

In this example, the hydroxypolyethylene oxide block copolymer was a hydroxypolyethylene oxide block copolymer having a trade name of Ethylan NS-500 LQ.

In this example, the dispersant was prepared from Guangyuan Yinong GY-D800 and Daatlox 4913 in the ratio of 1: 1 in a mixed combination.

In the embodiment, the defoaming agent is an organic silicon defoaming agent, and the specific mark is Mi image SAG 672; the specific use method of the defoaming agent is that the organic silicon defoaming agent is mixed with deionized water firstly, and the weight ratio of the two is 10: 1, and then mixed into the whole system.

In this embodiment, the thickener is lithium magnesium silicate.

In this embodiment, the bacteriostatic agent is isothiazolinone.

Example 3

This example provides an avermectin composition comprising:

the embodiment provides an avermectin suspending agent, and the preparation process of the suspending agent is as follows:

s01 raw material mixing

15.5t of corn starch, 2.5t of soybean cake powder, 950kg of yeast powder, 600kg of defoaming agent, 25kg of caustic soda flakes, 1.5kg of cobalt chloride, 2kg of sodium molybdate, 500g of manganese sulfate, 3.5kg of amylase and 40m of water are put into a feeding pool³Stirring to be milky;

s02 fermentation

Pumping into a fermentation tank by using a feeding pump, pressing 8000 units of actinomycetes to the fermentation tank from a distributor, adjusting the pH value in the fermentation tank to 6.8, opening front and rear valves of a steam filter to introduce steam, adjusting the opening of a valve to enable a pressure gauge on a precision filter to display 0.13MPa, then directly introducing steam with 0.4MPa and 150 ℃ into the fermentation tank, maintaining the pressure and continuously sterilizing for 0.5h when the temperature on the fermentation tank displays 120 ℃ and the pressure gauge displays 0.13MPa, then introducing circulating water into an inner coil and an outer coil of the fermentation tank to enable the temperature in the fermentation tank to reach 28.5 ℃, introducing compressed air into the fermentation tank, and discharging to the fermentation tank after sampling and checking are qualified after running for 540 h;

preparation of S03 preparation

Adding the rest components into the fermentation liquor, then crushing, and using a double-effect falling film evaporator to remove the excess moisture from the crushed material according to the requirement of 1% of the abamectin content.

In the invention, an ultrasonic nano crusher is used for crushing treatment, and the type of the ultrasonic nano crusher is JY-3500.

In this example, the hydroxypolyethylene oxide block copolymer is a hydroxypolyethylene oxide block copolymer of a brand number of ethyl lan NS-500LQ and a hydroxypolyethylene oxide block copolymer of a brand number of 1603# in a mass ratio of 7: 2, or a mixture thereof.

In the embodiment, the dispersant is prepared from German Wolkel VOK-G5000, Guangyuan Yinong GY-D800 according to the weight ratio of 1: 1 in a mixed combination.

In the embodiment, the defoaming agent is an organic silicon defoaming agent, and the specific trademark is Mitu SAG 672.

In this embodiment, the thickener is lithium magnesium silicate.

In this embodiment, the bacteriostatic agent is isothiazolinone.

Example 4

This example provides an avermectin composition comprising:

the embodiment provides an avermectin suspending agent, and the preparation process of the suspending agent is as follows:

s01 raw material mixing

15.5t of corn starch, 2.5t of soybean cake powder, 950kg of yeast powder, 600kg of defoaming agent, 25kg of caustic soda flakes, 1.5kg of cobalt chloride, 2kg of sodium molybdate, 500g of manganese sulfate, 3.5kg of amylase and 40m of water are put into a feeding pool³Stirring to be milky;

s02 fermentation

Pumping into a fermentation tank by using a feeding pump, pressing 8000 units of actinomycetes to the fermentation tank from a distributor, adjusting the pH value in the fermentation tank to 6.8, opening front and rear valves of a steam filter to introduce steam, adjusting the opening of a valve to enable a pressure gauge on a precision filter to display 0.13MPa, then directly introducing steam with 0.4MPa and 150 ℃ into the fermentation tank, maintaining the pressure and continuously sterilizing for 0.5h when the temperature on the fermentation tank displays 120 ℃ and the pressure gauge displays 0.13MPa, then introducing circulating water into an inner coil and an outer coil of the fermentation tank to enable the temperature in the fermentation tank to reach 28.5 ℃, introducing compressed air into the fermentation tank, and discharging to the fermentation tank after sampling and checking are qualified after running for 540 h;

preparation of S03 preparation

Adding the rest components into the fermentation liquor, then crushing, and using a double-effect falling film evaporator to remove the excess moisture from the crushed material according to the requirement of 1% of the abamectin content.

In the invention, an ultrasonic nano crusher is used for crushing treatment, and the type of the ultrasonic nano crusher is JY-3500.

In this example, the hydroxypolyethylene oxide block copolymer was a hydroxypolyethylene oxide block copolymer having a trade name of Ethylan NS-500 LQ.

In this example, the dispersant was prepared from Guangyuan Yinong GY-D800 and Daatlox 4913 in the ratio of 1: 1 in a mixed combination.

In the embodiment, the defoaming agent is an organic silicon defoaming agent, and the specific mark is Mi image SAG 672; the specific use method of the defoaming agent is that the organic silicon defoaming agent is mixed with deionized water firstly, and the weight ratio of the two is 10: 1, and then mixed into the whole system.

In this example, the thickener is xanthan gum.

In this embodiment, the bacteriostatic agent is isothiazolinone.

Performance testing

Test 1: and (3) suspension rate determination: measurements were carried out according to GB/T14825-2006.

And (3) testing 2: the deposition conditions after standing at high temperature (40 ℃) for 6 months are divided into non-deposition, partial deposition and total deposition.

And (3) testing: the examples after being placed at high temperature (40 ℃) for 6 months are stirred, the stirring mode is wood stick stirring, stirring is carried out for 6 circles in one minute, stirring is carried out for one minute, the uniform dispersion condition after stirring is checked, and the examples are divided into dispersion, most of uniform dispersion and small of uniform dispersion.

According to the embodiment, the magnesium lithium silicate is selected as the thickening agent, and the specific suspending agent, the defoaming agent and the like are matched, so that the prepared suspending agent has good suspension property, but the magnesium lithium silicate can be penetrated by water molecules in water and has strong stability, so that the inventor finds that when the suspending agent prepared by the magnesium lithium silicate is used immediately or stored for a short time, the product has good stability and the suspension property is beneficial; however, after long-term standing, the suspension stability of the pesticide is gradually transferred to the precipitation stability, and once the precipitation is generated, the difficulty of transferring the pesticide to the suspension is far greater than that of pesticide preparations using lithium magnesium silicate. The present inventors have found that a preparation method using a specific dispersant and a specific suspending agent can solve this problem; the colloid structure is firm and the yield value is large because of the existence of moisture before the finished product is prepared; the performance is stable; however, with the loss of water and the combination of the specific dispersant, the comb-shaped structure of the dispersant can reduce the flexibility value of the magnesium lithium silicate colloid because the acting force of the dispersant is greater than that of the magnesium lithium silicate with the loss of water, thereby alleviating the problems.

Indoor bioassay test report for pesticides

1.0% Abamectin SC on diamondback moth

Indoor biological activity assay

1 purpose of the test:

virulence of 1.0% avermectin suspension SC on Lepidoptera Plutella xylostella (Plutella xylostella L.) larvae

2 conditions of the test

2.1 test targets:

diamondback moth (Plutella xylostella L.) larva with substantially consistent size and health, and average weight of 0.0015 + -0.0001 g/head.

2.2 culture conditions:

the insect rearing room conditions are as follows: feeding fresh radish seedlings at the temperature of 24-26 ℃ and the relative humidity of 70-80% in an illumination period of L: D ═ 14: 10.

2.3 instruments and equipment:

electronic balance (sensing 0.0001), volumetric flask, beaker, pipettor, tweezers, filter paper, marker pen, stopwatch, temperature and humidity control equipment, etc.

Design of the experiment

3.1 reagents

3.1.1 test agents:

the invention example 1 is 1 with 1.0% avermectin suspending agent SC.

3.1.2 other reagents:

acetone, tween 80, distilled water, and the like.

3.2 test treatment

3.2.1 dose settings:

the test agent is directly diluted into 1500 times of solution and 3000 times of solution respectively by using clear water. Clear water was used as a blank control.

3.2.2 test replicates:

each concentration was tested in 4 replicates, each replicate treating 15 test insects.

3.3 mode of treatment

3.3.1 treatment time and number:

test treatments were performed on 24 days at 5 months 2021.

3.3.2 instruments used and methods of administration:

the instruments used include beaker, tweezers, stopwatch, finger tube (diameter 1.8cm, length 8cm), etc., and the soaking time is 30 seconds respectively by the leaf soaking method.

4 test method:

the leaf soaking method comprises soaking fresh caulis et folium Brassicae Capitatae in medicinal liquid for 30 s, air drying on absorbent paper, placing into insect box, and inoculating 15 heads of insect test. And (3) feeding and observing under the conditions that the temperature is 24-26 ℃, the relative humidity is 70-80% and the illumination period is L: D ═ 14: 10.

Referring to the agricultural industry standard NYT 1154.14-2008 of the people's republic of China, part 14 of the indoor bioassay test criteria for pesticides: insecticidal activity test, leaf soaking method.

5 data investigation and statistical analysis

5.1 survey methods and grading standards:

the test investigation method is that a skilled technician lightly touches the insect body with a writing brush, and the person who does not move or can not normally crawl is counted as death.

5.2 investigation time and number of surveys:

the test was investigated twice, i.e. 24 and 48 hours after treatment to check for test insect mortality.

5.3 statistical analysis of data

The survival of the test insects is investigated, and the corrected mortality rate is calculated according to the Abbott formula (1).

In the formula: mm-corrected mortality

Mt-treatment group mortality

Mc-mortality of control group

The results of the statistical analysis are shown in Table 1.

5.4 other cases

None.

6 results analysis and discussion

6.1 evaluation of drug

TABLE 11.0% Abamectin SC Table for indoor bioassay results of Plutella xylostella

2021.5.

From the indoor bioassay result of the 1.0% avermectin suspending agent SC on the diamondback moth larvae, no matter the dilution is 1500 times or 3000 times after the administration for 24 hours, the toxicity to the diamondback moth larvae is not obviously different. After the two agents are diluted by 1500 times and 3000 times for 48 hours, the toxicity of the two agents to the test insects reaches more than 85 percent.

6.2 problem analysis

The leaf soaking method adopted in the test reflects the main effects of contact toxicity and partial stomach toxicity of the medicament.