阅读说明：本技术 细胞迁移实验用打孔器 (Puncher for cell migration experiment ) 是由 孙炳伟 杨云稀 丁慎平 于 2020-06-09 设计创作，主要内容包括：本申请涉及一种细胞迁移实验用打孔器,包括：底座；抵接台,设置在所述底座上；打孔组件,与所述抵接台之间形成间隙以放置待打孔目标；其中,打孔组件包括与所述底座连接且具有收容腔的外壳、及设置在所述外壳内且可相对于所述外壳移动的打孔件,所述外壳的一端设置在所述底座上且开设有缺口,所述缺口的高度大于所述抵接台的高度以与所述抵接台之间形成间隙；驱动所述打孔件朝向所述待打孔目标移动,直至所述打孔件穿过所述待打孔目标且与所述抵接台抵持,以在所述待打孔目标上形成孔洞。其方便快捷,在提高打孔效率的同时,也提高了打孔的精度。(The application relates to a cell migration is hole puncher for experiments, includes: a base; the abutting table is arranged on the base; the punching assembly and the abutting table form a gap for placing a target to be punched; the punching assembly comprises a shell which is connected with the base and is provided with an accommodating cavity, and a punching piece which is arranged in the shell and can move relative to the shell, one end of the shell is arranged on the base and is provided with a notch, and the height of the notch is greater than that of the abutting table so as to form a gap with the abutting table; and driving the punching piece to move towards the target to be punched until the punching piece penetrates through the target to be punched and abuts against the abutting table so as to form a hole in the target to be punched. The punching device is convenient and fast, and improves punching precision while improving punching efficiency.)

1. A punch for cell migration experiments, comprising:

a base;

the abutting table is arranged on the base;

the punching assembly and the abutting table form a gap for placing a target to be punched;

the punching assembly comprises a shell which is connected with the base and is provided with an accommodating cavity, and a punching piece which is arranged in the shell and can move relative to the shell, one end of the shell is arranged on the base and is provided with a notch, and the height of the notch is greater than that of the abutting table so as to form a gap with the abutting table; and driving the punching piece to move towards the target to be punched until the punching piece penetrates through the target to be punched and abuts against the abutting table so as to form a hole in the target to be punched.

2. The cell migration assay punch sheet of claim 1, further comprising a drive member coupled to the punch member, the drive member configured to drive the punch member to move relative to the housing.

3. The punch sheet for cell migration experiments according to claim 2, wherein the punch assembly further comprises an elastic member abutting against the driving member; when external force is applied to enable the driving piece to drive the punching piece to move towards the abutting joint table, the elastic piece is compressed; when the external force is cancelled, the elastic piece drives the driving piece to move towards the direction far away from the butt joint table under the action of the elastic force of the elastic piece, and then the punching piece is driven to move towards the direction far away from the butt joint table.

4. The hole punch sheet for cell migration experiments according to claim 3, wherein the hole punch assembly further comprises a connecting sheet connected to the elastic member, and a through hole is formed in the connecting sheet for allowing the hole punch to pass through.

5. The punch sheet for cell migration experiments according to claim 1, wherein a slide groove is provided on one of the housing and the driving member, and a slide block slidably engaged with the slide groove is provided on the other of the housing and the driving member.

6. The punch sheet for cell migration experiments according to claim 1, wherein at least two punch members are provided.

7. The punch sheet for cell migration experiments according to claim 6, wherein at least two of said punch members are disposed at equal intervals.

8. The punch sheet for cell migration experiments according to claim 1, wherein the target to be punched is an agarose sheet.

Technical Field

The invention relates to a puncher for cell migration experiments, and belongs to the technical field of biological detection equipment.

Background

Cell culture dishes are generally used for cell detection, and most of the cell culture dishes are agarose plates cast with agarose. The specific shape of the agarose plates varies from cell to cell. For example, neutrophils, whose chemotactic activity is often detected on agarose strips, require the perforation of the agarose strips according to the specifications. The punching on the previous agarose plates is carried out manually, the precision is poor, and the hole spacing is not easy to standardize.

Disclosure of Invention

The invention aims to provide a puncher for cell migration experiments, which can improve punching precision and punching standard, and is quick and convenient.

In order to achieve the purpose, the invention provides the following technical scheme: a cell migration experiment punch, comprising:

a base;

the abutting table is arranged on the base;

the punching assembly and the abutting table form a gap for placing a target to be punched;

the punching assembly comprises a shell which is connected with the base and is provided with an accommodating cavity, and a punching piece which is arranged in the shell and can move relative to the shell, one end of the shell is arranged on the base and is provided with a notch, and the height of the notch is greater than that of the abutting table so as to form a gap with the abutting table; and driving the punching piece to move towards the target to be punched until the punching piece penetrates through the target to be punched and abuts against the abutting table so as to form a hole in the target to be punched.

Further, the punch sheet for cell migration experiment further comprises a driving member connected with the punching member, and the driving member is used for driving the punching member to move compared with the shell.

Further, the punching assembly further comprises an elastic piece abutted with the driving piece; when external force is applied to enable the driving piece to drive the punching piece to move towards the abutting joint table, the elastic piece is compressed; when the external force is cancelled, the elastic piece drives the driving piece to move towards the direction far away from the butt joint table under the action of the elastic force of the elastic piece, and then the punching piece is driven to move towards the direction far away from the butt joint table.

Furthermore, the punching assembly further comprises a connecting sheet connected with the elastic piece, and a through hole for allowing the punching piece to pass through is formed in the connecting sheet.

Furthermore, one of the shell and the driving piece is provided with a sliding groove, and the other of the shell and the driving piece is provided with a sliding block in sliding fit with the sliding groove.

Further, the punching pieces are provided with at least two.

Further, at least two of the punching members are arranged at equal intervals.

Further, the target to be punched is an agarose sheet.

The invention has the beneficial effects that: through being provided with the butt joint platform and the subassembly that punches, the subassembly that punches includes the piece that punches that can remove for the shell, and the target that punches is treated to the drive and is removed and break through the target of waiting to punch and support with the butt platform and hold until the piece that punches to form the hole on treating the target of punching, convenient and fast when improving punching efficiency, also improved the precision of punching.

The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.

Drawings

FIG. 1 is a schematic diagram of the structure of a punch for cell migration experiments according to the present invention.

FIG. 2 is a schematic view of another structure of the punch for cell migration experiment according to the present invention.

Fig. 3 is a partial structural diagram of fig. 2.

Detailed Description

The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

Referring to fig. 1 to 3, a hole puncher for cell migration experiments in a preferred embodiment of the invention includes a base 1, an abutting table 2 disposed on the base 1, and a hole punching assembly 3 forming a gap with the abutting table 2 for placing an object to be punched, wherein the object to be punched is an agarose sheet in this embodiment, and indeed, the object to be punched is other objects in other embodiments, which are not specifically limited herein, according to the actual situation.

The punching assembly 3 includes a housing 31 connected to the base 1 and having a receiving cavity, and a punching member disposed in the housing 31 and movable relative to the housing 31, wherein one end of the housing 31 is disposed on the base 1 and has a gap 32, and the height of the gap 32 is greater than the height of the abutment 2 to form a gap with the abutment 2. And driving the punching piece to move towards the target to be punched until the punching piece penetrates through the target to be punched and abuts against the abutting table 2 so as to form a hole on the target to be punched. Wherein, punch the piece and be provided with two at least, at least two punch a equidistance setting. In this embodiment, the number of the punching pieces is three, and the three punching pieces are arranged at equal intervals. The purpose of this is to: can once only beat a plurality of holes on agarose piece, and the distance between every two adjacent holes equals, when improving punching efficiency, can also improve the accuracy of the distance between the hole, kill many birds with one stone. Indeed, in other embodiments, the number of the punching elements is determined according to the actual size of the agarose plate or the actual requirement, and is not specifically limited herein, and is determined according to the actual situation.

The punch sheet for cell migration experiment further comprises a driving member 4 connected to the punch member, wherein the driving member 4 is used for driving the punch member to move relative to the housing 31. In order to enable the driving member 4 to drive the punching member to move along the housing 31 better, one of the housing and the driving member 4 is provided with a sliding groove 41, and the other of the housing and the driving member 4 is provided with a sliding block which is in sliding fit with the sliding groove 41. In this embodiment, the housing 31 is provided with a slider, the driving member 4 is provided with a sliding groove 41, and the slider and the housing 31 are integrally formed, so that the production difficulty can be reduced, and the production efficiency can be improved. Indeed, the slider may be separately disposed from the housing 31 and then fixedly connected thereto by bonding, welding, or clamping, which is not specifically limited herein and depends on practical situations.

The user can direct application of force in driving piece 4 and then drive driving piece 4 is with the drive piece of punching, can also be connected driving piece 4 and outside application of force mechanism, and outside application of force mechanism drive driving piece 4 quick travel, and then can form the hole on the agarose piece fast for the hole is comparatively level and smooth, and can make the agarose piece after punching not have the residue.

The perforating assembly 3 further comprises an elastic member 33 abutting against the driving member 4; when an external force is applied so that the driving piece 4 drives the punching piece to move towards the abutting table 2, the elastic piece 33 is compressed; when the external force is removed, the elastic member 33 drives the driving member 4 to move towards the direction away from the abutting table 2 under the elastic force action, and then drives the punching member to move towards the direction away from the abutting table 2. The housing 31 has a body 311 and an upper end 312 connected to the body 311, and at least a part of the driving member 4 is disposed in the body 311 and abuts against the upper end 312. The punching assembly 3 further comprises a connecting piece 313 connected with the elastic piece 33, the connecting piece 313 is arranged at the lower end of the body 311 and is fixedly connected with the shell 31, and a through hole for allowing the punching piece to pass through is formed in the connecting piece 313.

In summary, the following steps: through being provided with butt joint platform 2 and the subassembly 3 that punches, the subassembly 3 that punches includes the piece that punches that can remove for shell 31, and the drive is punched the piece orientation and is waited to punch the target and remove and to break through and wait to punch the target and support with butt platform 2 and hold to form the hole on waiting to punch the target, convenient and fast when improving punching efficiency, also improved the precision of punching.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.