阅读说明：本技术 澳桉酚在除草剂中的应用 (Application of eudesmol in herbicide ) 是由 叶火春 刘霞 王敬茹 闫超 冯岗 于 2021-10-12 设计创作，主要内容包括：本发明涉及除草剂技术领域,尤其涉及澳桉酚在除草剂中的应用,澳桉酚在防治杂草中的应用,本发明提供的化合物对农业杂草具有显著的抑制作用,为农业杂草防治中提供了一种天然源除草活性物质；该化合物可用于防治马唐、茼麻、稗草和反枝苋等多种重要的农业杂草；将其作为除草剂,其高效、低毒、安全等特点符合当前新农药创制的要求。(The invention relates to the technical field of herbicides, in particular to application of eudesmol in herbicides and application of eudesmol in weed control, and the compound provided by the invention has a remarkable inhibition effect on agricultural weeds and provides a natural source weeding active substance for agricultural weed control; the compound can be used for preventing and controlling various important agricultural weeds such as crab grass, crowndaisy hemp, barnyard grass, redroot amaranth and the like; the herbicide has the characteristics of high efficiency, low toxicity, safety and the like, and meets the requirements of the current new pesticide creation.)

1. The application of the eudesmol in the herbicide is characterized in that the application of the eudesmol in the weed control.

2. The use of eudesmol as claimed in claim 1, wherein said weeds are agricultural weeds.

3. The use of eudesmol as claimed in claim 2, wherein said agricultural weeds comprise: barnyard grass, cyperus rotundus, sudangrass, garland hemp, amaranth, moleplant seed, redroot amaranth, water barnyard grass, goosegrass, crab grass and broadleaf fairy grass.

4. The use of eudesmol as claimed in claim 1, wherein said herbicide comprises said eudesmol and an agriculturally acceptable adjuvant.

5. The use of the eudesmol as claimed in claim 4, wherein the effective amount of said eudesmol in said herbicide is 1-10 mg/mL.

6. The use of eudesmol as claimed in claim 4, wherein said agriculturally acceptable adjuvant is one or more of a diluent, a binder, a disintegrant, a lubricant, a pH adjusting agent, a co-solvent or an antioxidant.

7. The use of eudesmol as claimed in claim 4, wherein said herbicide is in the form of emulsion, suspension, wettable powder, granule, aqueous solution, poison bait, mother powder.

Technical Field

The invention relates to the technical field of herbicides, in particular to application of eudesmol in herbicides.

Background

Weeds are a large group of biological disasters that severely threaten crop production. The harm of weeds is manifested in direct competition with crops for nutrients, moisture, light and growing space, resulting in reduced crop yields. The use of the herbicide can not only effectively prevent and kill farmland weeds, improve the quality and the yield of crops, but also save a large amount of labor cost. However, the long-term use of chemical herbicides in large quantities has made the problem of weed resistance prominent. By 2016 (11) months, 476 biotypes of 251 weeds (146 dicots, 105 monocots) had developed resistance to 161 products of 26 classes of chemical herbicides worldwide. In addition, due to improper use, fog drop drifting, leaching, liquid medicine volatilization and the like, the chemical herbicide not only seriously affects the growth and development of crops, but also brings the problems of residual toxicity and phytotoxicity, yield reduction of next crops, land degradation and the like. Some highly toxic herbicides, such as paraquat, are life threatening when they are ingested by humans. Currently, over 100 chemical herbicides are banned or deregistered in over 30 countries around the world. With the need of agricultural sustainable development and the improvement of environmental awareness, the development and application of novel biopesticides to control the occurrence of diseases, pests and weeds is one of the key points of future pesticide research.

Eudesmol, also known as 4- (1-methylethyl) -phenol, has the following chemical structure:

the essential oil exists in essential oils of various plants, has simple chemical structure and is easy to synthesize. The method can provide a material guarantee for developing a novel botanical herbicide by fully utilizing the eudesmol in the follow-up process. Through retrieval, no report about the agricultural activity of the eudesmol exists at home and abroad at present, and no report about the herbicidal activity of the eudesmol exists.

Disclosure of Invention

The invention aims to solve the defects in the prior art and provides application of eudesmol in herbicides.

Application of the eudesmol in herbicide and application of the eudesmol in weed control.

Preferably, the weeds are agricultural weeds.

Preferably, the agricultural weeds comprise: barnyard grass, cyperus rotundus, sudangrass, garland hemp, amaranth, moleplant seed, redroot amaranth, water barnyard grass, goosegrass, crab grass and broadleaf fairy grass.

Preferably, the herbicide comprises the eudesmol and agriculturally and pharmaceutically acceptable auxiliary materials.

Preferably, the effective dose of the eudesmol in the herbicide is 1-10 mg/mL.

Preferably, the agriculturally and pharmaceutically acceptable auxiliary materials are one or more of diluents, binders, disintegrants, lubricants, pH regulators, cosolvents or antioxidants.

Preferably, the herbicide is in the form of emulsion, suspending agent, wettable powder, granules, aqueous agent, poison bait, mother liquor and mother powder.

The invention has the beneficial effects that: the compound provided by the invention has a remarkable inhibiting effect on agricultural weeds, and provides a natural source weeding active substance for preventing and controlling the agricultural weeds; the compound can be used for preventing and controlling various important agricultural weeds such as crab grass, crowndaisy hemp, barnyard grass, redroot amaranth and the like; the herbicide has the characteristics of high efficiency, low toxicity, safety and the like, and meets the requirements of the current new pesticide creation.

Detailed Description

The present invention will be further illustrated with reference to the following specific examples.

Application of the eudesmol in herbicide and application of the eudesmol in weed control.

Further, the weeds are agricultural weeds.

Further, agricultural weeds include: barnyard grass, cyperus rotundus, sudangrass, garland hemp, amaranth, moleplant seed, redroot amaranth, water barnyard grass, goosegrass, crab grass and broadleaf fairy grass.

Further, the herbicide comprises the eudesmol and agriculturally and pharmaceutically acceptable auxiliary materials.

Further, the effective dose of the eudesmol in the herbicide is 1-10 mg/mL.

Furthermore, the agriculturally and pharmaceutically acceptable auxiliary materials are one or more of diluents, binders, disintegrants, lubricants, pH regulators, cosolvents or antioxidants.

Furthermore, the dosage form of the herbicide is emulsion, suspending agent, wettable powder, granules, aqueous agent, poison bait, mother liquor and mother powder.

The above-mentioned Audretol can be obtained by chemical synthesis or by separation from olive oil. The compound is white powder with CAS number of 99-89-8-4 and molecular formula of C₁₀H₁₄O, molecular weight 150.22.

In order to evaluate the agricultural weeding activity of the eudesmol, the invention adopts a seed germination method to measure the inhibition effect of the compound on the root length and the stem length of agricultural weeds to evaluate the weeding activity of the compound on the agricultural weeds, and further verifies the weeding effect of the compound through a living potted plant, thereby evaluating the compound.

Sources of eudesmol

Audrecol, with a purity of greater than 98%, was purchased from Beijing Bailingwei science and technology Co.

Determination of inhibitory activity of eudesmol on agricultural weeds

1. Weed to be tested

Echinochloa crusgalli (Echinochloa crusgalli), Cyperus rotundus (Cyperus rotundus), Salvia miltiorrhiza (Sorghum sudanense), Abutili coronaria (Abutilon hybridum), Amaranthus mangostana (Amaranthus tricolor), Euphorbia lathyris (Leptochloa chinensis), Amaranthus retroflexus (Amaranthus retroflexus), Echinochloa crusgalli (Echinochloa phyllopogon), Rhizophora viridis (Chlorris virgata), Eleusines indica (Eleusines), Digitaria sanguinalis (Digitaria sanguinalis) and Borreria latifolia (Borreria latifolia), all of which are provided by the group of agricultural chemical biology and application topics of the environmental and plant protection institute of the Chinese institute of Tropical sciences.

2. Test method

2.1 in vitro Activity assay

In order to test the herbicidal activity of the eudesmol, the present invention evaluated the herbicidal activity of the compounds of the present invention against agricultural weeds by using a seed germination method. Dissolving the eudesmol with constant volume in ethanol, diluting to 1mg/mL for test, repeating the treatment for 3 times, and repeating for 20 weed seeds. The culture dish is put into a constant temperature incubator [ (28 +/-1) DEG C, the relative humidity is 80-85%, and the L/D is 12h/12h ] for culture, the root length and the stem length of the seedling are measured when the blank control grows to the proper length, and the inhibition rate is calculated according to the following formula.

Inhibition rate ═ control (root or stem length) -treatment (root or stem length) ]/control (root or stem length) × 100%

2.2 data analysis

The weeding activity results of the eudesmol on 12 weeds to be tested are shown in table 1, and experiments prove that the eudesmol provided by the invention has strong root length and stem length inhibition effects on barnyard grass, sudancao, garland hemp, amaranth, redroot amaranth, cockspur grass and crab grass when the test concentration is 1mg/mL, the inhibition rates are all more than 90%, and particularly, the root length and stem length of the eudesmol are all completely inhibited on the barnyard grass and the amaranth; the herbicide also has certain root length and stem length inhibiting effects on moleplant seed, tiger tail grass, eleusine indica and broadleaf fenghuacao, but the sensitivity of the herbicide is lower than that of the weeds; the herbicide activity to cyperus rotundus is lowest, and the inhibition rate of root length and stem length is only 74.72% and 57.26%.

TABLE 1 herbicidal Activity of Audrecol on 10 weeds

2.3 potting test method

The reference method for the in vivo pot culture test of eudesmol on weeds is slightly improved: taking barnyard grass, garland hemp and amaranth as weeds to be tested, disinfecting and soaking seeds in warm water at 50 ℃ for 2h, wrapping the seeds with a wet towel, placing the seeds into a constant-temperature incubator [ (28 +/-1) DEG C, the relative humidity of 80-85% and the L/D of 12h/12h ] for accelerating germination for 24h, dibbling the seeds after the seeds are whitened in plastic pots filled with planting soil, uniformly dibbling 15-20 seeds in each pot, completely sprinkling the planting soil with the thickness of 1cm, properly watering to keep the humidity, placing the plants in a greenhouse with the temperatures of [ (26 +/-1) DEG C, the relative humidity of 70-90% and the L/D of 12h ], when 2-3 new leaves grow on the plants, uniformly spraying liquid medicines with the pipermide treatment concentrations of 10, 5 and 2.5mg/mL to the treated plants by using a small-sized spray pot, spraying 2-3mL each time, using clean water as blank control and ethanol as solvent control, the treatment concentration of the control medicament 5% eucalyptus oil soluble liquid is 1mg/mL, each treatment is repeated for 3 times, the symptoms of treated plants are regularly observed and recorded, the fresh weight of the overground part is weighed 7 days after treatment, and the prevention and treatment effect is calculated.

Control effect ═ control (fresh weight) -treatment (fresh weight) ]/control (fresh weight) × 100%

The in-vivo pot culture result shows that the control effect of 10mg/ml eudesmol treated cockspur grass, abutilon, amaranth is respectively 88.4%, 84.9% and 88.3% after 7 days, the weeding activity is higher, and the difference of the weeding activity and the control medicament 5% eucalyptus oil soluble liquid is not significant. And the control effect of the eudesmol treated by 5mg/ml on three kinds of weeds reaches more than 65% (tables 2 and 3). In addition, toxicity symptoms of the eudesmol on weeds are observed, the weeds show symptoms of lodging and withered leaves after being treated for 2 hours by 10mg/ml of the eudesmol and a control agent, and the treatment effects of 5mg/ml and 2.5mg/ml of the eudesmol are not obvious; after 24 hours, all the medicaments are subjected to treatment, and lodging, withered leaves and greenness generation are caused; after which the symptoms are more pronounced.

TABLE 2 potted plant test results of eudesmol for barnyard grass and crownday chrysanthemum

TABLE 3 potted plant test results of Audrecol vs. amaranth

The invention discloses application of eudesmol in preventing and controlling agricultural weeds. The test proves that the eudesmol has excellent herbicidal activity. When the tested concentration is 1000mg/L, the compound has stronger root length and stem length inhibition effects on barnyard grass, sudancao, crowndaisy hemp, amaranth, redroot pigweed, barnyard grass and crab grass, the inhibition rate is over 90 percent, and particularly, the root length and stem length of the compound are completely inhibited on the barnyard grass and the amaranth; pot experiments further verify that the compound has a strong contact poisoning effect on barnyard grass, crowndaisy hemp and amaranth, and the control effect of the compound is not significantly different from that of a control medicament 5% eucalyptus oil soluble liquid under the same concentration. The above results show that the eudesmol has a broad-spectrum herbicidal effect and can be deeply developed as a contact herbicide.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.