阅读说明：本技术 植物蒸腾抑制剂及其制备方法 (Plant transpiration inhibitor and preparation method thereof ) 是由 李华玮 齐向军 陈红飞 陈治毅 于 2021-06-22 设计创作，主要内容包括：本发明公开了一种植物蒸腾抑制剂及其制备方法,原料组分包括：水杨酸·γ-氨基丁酸衍生物、褐藻酸胶、赋形剂。制备步骤：S1：制备水杨酸·γ-氨基丁酸衍生物溶液；S2、向S1步骤制备的溶液中加入表面活性剂6-14％,60℃恒温搅拌至各组分完全溶解,静置待用；S3、依次将褐藻酸胶3-20％,稳定剂3-10％缓慢加入S2制备的溶液中,并在55-60℃范围内以100转/分钟的速度进搅拌30-40分钟,使溶液呈胶悬状；S4、向S3制备的溶液中加入防腐剂3-10％,并降低转速至60转/分钟搅拌至完全溶解,本发明的蒸腾抑制剂具有调节作物叶片气孔闭合的作用,润湿铺展能力强,抗蒸腾效果好,同时具有激活植物自身的免疫系统和防御机制的功能。(The invention discloses a plant transpiration inhibitor and a preparation method thereof, and the plant transpiration inhibitor comprises the following raw material components: salicylic acid-gamma-aminobutyric acid derivative, alginate gel and excipient. The preparation method comprises the following steps: s1: preparing a salicylic acid-gamma-aminobutyric acid derivative solution; s2, adding 6-14% of surfactant into the solution prepared in the step S1, stirring at a constant temperature of 60 ℃ until all components are completely dissolved, and standing for later use; s3, sequentially adding 3-20% of alginate gum and 3-10% of stabilizing agent slowly into the solution prepared in the S2, and stirring at the speed of 100 revolutions per minute within the range of 55-60 ℃ for 30-40 minutes to enable the solution to be in a colloidal suspension state; s4, adding 3-10% of preservative into the solution prepared by the S3, and reducing the rotating speed to 60 r/min to stir until the preservative is completely dissolved.)

1. A plant transpiration inhibitor, characterized by: the raw material components comprise: salicylic acid-gamma-aminobutyric acid derivative, alginate gel and excipient.

2. A plant transpiration inhibitor according to claim 1, wherein: the raw material components comprise 5-30% of salicylic acid gamma-aminobutyric acid derivatives, 3-20% of alginate gel and the balance of excipients in percentage by mass.

3. A plant transpiration inhibitor according to claim 1, wherein: the salicylic acid gamma-aminobutyric acid derivative is alkali metal salt or alkaline earth metal salt.

4. A plant transpiration inhibitor according to claim 1, wherein: the algin comprises sodium alginate or potassium alginate.

5. A plant transpiration inhibitor according to claim 2, wherein: the excipient is solvent, surfactant, antiseptic and stabilizer.

6. The plant transpiration inhibitor according to claim 5, wherein: the surfactant comprises one or a mixture of more than two of nonionic surfactant, anionic surfactant, cationic surfactant and amphoteric surfactant.

7. The plant transpiration inhibitor according to claim 5, wherein: the raw material components comprise, by mass, 5-30% of salicylic acid gamma-aminobutyric acid derivatives, 3-20% of alginate gel, 6-14% of surfactants, 3-10% of preservatives, 1-10% of stabilizers and the balance of solvents.

8. A preparation method of a plant transpiration inhibitor is characterized by comprising the following steps: the method comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid derivative solution, heating a solvent to 70-100 ℃, sequentially adding salicylic acid, gamma-aminobutyric acid and alkali metal or alkaline earth metal into the solvent, and heating and stirring at the speed of 200 revolutions per minute for 2 hours until the salicylic acid, the gamma-aminobutyric acid and the alkali metal or alkaline earth metal are completely dissolved;

s2, adding a surfactant into the solution prepared in the step S1, stirring at a constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially and slowly adding the algin and the stabilizer into the solution prepared in the S2, stirring the solution for 30 to 40 minutes at the speed of 100 revolutions per minute within the range of 55 to 60 ℃, and adjusting the pH to 8 to 10 to enable the solution to be in a colloidal suspension state;

s4, adding the preservative into the solution prepared in the S3, reducing the rotating speed to 60 revolutions per minute, stirring until the preservative is completely dissolved, cooling and filling.

9. The method for preparing a plant transpiration inhibitor according to claim 8, wherein: the raw material components comprise, by mass, 5-30% of salicylic acid gamma-aminobutyric acid derivatives, 3-20% of alginate gel, 6-14% of surfactants, 3-10% of preservatives, 1-10% of stabilizers and the balance of solvents.

Technical Field

The invention relates to the technical field of agricultural chemistry, in particular to a plant transpiration inhibitor and a preparation method thereof.

Background

The transpiration inhibitor is a general name of substances which act on the surfaces of plant leaves, can effectively inhibit the transpiration intensity of plants and reduce the water loss of the plants, plays an important role in drought resistance, water saving, disaster reduction and yield preservation in agricultural production, and is a novel drought resistance and biochemical control technology. The transpiration inhibitor can be divided into four categories according to different action characteristics, namely a film type, a reflection type, a metabolic type and a film and metabolism mixed type. The film type active ingredients are mostly organic high molecular compounds, and a soft and smooth film is formed on the leaf surface by spraying, so that water molecules are prevented from diffusing into the atmosphere, and the moisture entering the air by diffusing through air holes is greatly reduced. The reflection-type anti-transpirant utilizes the reflection of partial solar radiation energy by the reflecting material to reduce the absorption of solar radiation by the blades, thereby reducing the temperature of the blades and reducing transpiration. The metabolic type can close or reduce the opening of the stomata, thereby increasing the transpiration resistance of the stomata and inhibiting the loss of water in the plant body during transpiration.

The conventional film type plant transpiration inhibitor can increase the water potential of plant leaves and fruits, has good effects of improving crop yield, improving the survival rate of plant transplantation and cuttage, warming and moisturizing, resisting insect diseases, improving plant resistance and the like, is more effective than other two plant transpiration inhibitors under the condition of short-term drought, and is widely applied to the fields of ornamental and greening tree species, big tree transplantation, fruit, vegetable and flower fresh-keeping and the like.

Although the conventional film-type plant transpiration inhibitor has a relatively excellent effect, adopts a high-molecular polymer, has strong wetting and spreading capability when being sprayed on plant leaf surfaces, is not easy to drop into water drops, has high utilization rate, has a single disease and insect resistant action mode and has poor effect on plants infected with germs because the transpiration inhibition is carried out only in a film-forming physical mode.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide an environment-friendly plant transpiration inhibitor and a preparation method thereof, so as to overcome the problems that the anti-transpiration effect of the existing film type plant transpiration inhibitor is limited and the disease-resistant effect mode is single, and the film type plant transpiration inhibitor has the comprehensive functions of a film type plant transpiration inhibitor and a physiological and biochemical metabolic type plant transpiration inhibitor.

The purpose of the invention is realized by the following technical scheme:

a plant transpiration inhibitor comprises the following raw material components: salicylic acid-gamma-aminobutyric acid derivative, alginate gel and excipient.

The salicylic acid is named as o-hydroxybenzoic acid, a kind of micromolecular phenolic substance widely existing in plants, is an intermediate product of phenylalanine metabolic pathway, and belongs to a derivative of meat silicic acid. In the transpiration inhibitor, salicylic acid can trigger the molecular reaction in plant cells, and the bacteriostatic action of the plant cells is exerted by activating the immune system and defense mechanism of the plant, the salicylic acid-gamma-aminobutyric acid derivative has strong systemic property, can be absorbed by plant leaves and root systems and conveyed into the plant body to induce seedling cells to generate lignin, increase the thickness of cell walls, enable the cell walls to become hard and firm, and can adjust the stomata of the plant leaves to be closed, prevent the invasion of pathogenic bacteria, obstruct the invasion path of the pathogenic bacteria, reduce the morbidity, simultaneously slow down the transpiration, and in the rain-showering process, the catabolite of the catabolite can be directly absorbed and utilized by the plant, and has no residue and no pollution to the environment. Salicylic acid participates in various physiological and biochemical processes of plant transpiration, seed germination, flowering, fructification, stomatal closure, heat production and the like; inducing plants to generate various physiological characters such as disease resistance, salt resistance, cold resistance and the like, and also participating in the route of cyanogenic respiration resistance and non-phosphorylation of plant cell mitochondria, and improving the metabolic level of the jasminic acid in the plants.

In plants, r-aminobutyric acid plays a dual role as a metabolite and a signal substance, and is involved in many important physiological processes of plants. The gamma-aminobutyric acid promotes photosynthesis, influences energy metabolism, promotes vegetative growth and reproductive growth of plants, improves the resistance of the plants to different stresses (high temperature, low temperature, drought, salt damage and the like), and has the function of promoting the absorption of the plants to the medium and trace elements. Drought, one of the abiotic stresses, seriously affects the growth and development of plants. Gamma-aminobutyric acid is zwitterion and is easy to dissolve in water under the condition of physiological pH, and the gamma-aminobutyric acid is used as a micromolecular osmotic adjusting substance to reduce the osmotic potential in cytoplasm under the condition of water stress, so that the water retention property of cells is improved, the damage to plants caused by water shortage is relieved, and the gene expression related to the homeostasis of the cells, the removal of active oxygen, the stable protection of structural proteins, osmotic regulators, transport proteins and the like is up-regulated under the drought condition. Exogenous gamma-aminobutyric acid enables the plant to keep high relative water content, electrolyte leakage, lipid, superoxide and carbon metabolism are reduced, and membrane stability can be improved.

In addition, exogenous gamma-aminobutyric acid can also induce the activity increase of gamma-aminobutyric acid-T and alpha-valeric acid dehydrogenase, and the inhibition of GAD activity can increase gamma-aminobutyric acid and glutamic acid. Meanwhile, gamma-aminobutyric acid accelerates polyamine synthesis, inhibits polyamine decomposition, and further activates sigma-1-pyrroline-5-carboxylic acid synthetase, proline dehydrogenase and ornithine-sigma-aminotransferase activity, so that the gamma-aminobutyric acid preconcentrate is highly accumulated and metabolized. The gamma-aminobutyric acid can also increase the activities of catalase and peroxidase by promoting the expression of chlorophyll, improve the contents of proline and sugar, regulate permeation and reduce oxidation. Gamma-aminobutyric acid helps the defense of plants against external natural enemies. When insects feed, the mechanical cutting can stimulate the increase of Ca2+ in the plants due to the fact that the plants are injured to cause cell rupture and tissue injury, and the plants secrete gamma-aminobutyric acid under the stimulation of Ca2+ as a measure for resisting the feeding of the insects. No jasmonate signal is involved in the accumulation of gamma-aminobutyric acid in the process.

The alginate gel has excellent film-forming property, forms a transparent and soft film after being sprayed and applied, can obviously reduce the water loss of plants, but does not interfere the growth or normal respiration of the plants, can adjust the closure of the stomata of the leaves of the plants, obstruct the invasion path of pathogenic bacteria, prevent the invasion of the pathogenic bacteria, reduce the morbidity and simultaneously slow down the transpiration of the plant water. The compound has a synergistic effect with salicylic acid gamma-aminobutyric acid derivatives with different action modes; meanwhile, the excipient has the function of endowing the plant transpiration inhibitor with a specific stable form, is convenient to circulate and use, and enables the plant transpiration inhibitor to meet the requirements of dispersion and wetting of various application technologies.

The further technical scheme is that the raw material components comprise, by mass, 5-30% of salicylic acid gamma-aminobutyric acid derivatives, 3-20% of alginate gel and the balance of excipients.

The further technical scheme is that the salicylic acid gamma-aminobutyric acid derivative is alkali metal salt and/or alkaline earth metal salt in percentage by mass. The disease resistance of the salicylic acid-gamma-aminobutyric acid derivative is systemic disease resistance SAr acquired in the future, is also called induced systemic disease resistance ISr, and belongs to one of generalized biological control. The salicylic acid-gamma-aminobutyric acid derivative has a disease-resistant effect, and alkali metal or alkaline earth metal of the salicylic acid-gamma-aminobutyric acid derivative also has good effects of promoting roots, strengthening roots, promoting flowers, setting fruits, expanding fruits, coloring, increasing sugar, increasing fruit powder, increasing dry matter accumulation, increasing soluble matter content and the like.

The further technical scheme is that the algin comprises sodium alginate or potassium alginate. The sodium alginate and the potassium alginate have excellent film forming properties, form a transparent and soft film after being sprayed and applied, can remarkably reduce the water loss of plants, but do not disturb the growth or normal respiration of the plants, can adjust the closure of air holes of leaves of the plants, obstruct the invasion path of pathogenic bacteria, prevent the invasion of the pathogenic bacteria, reduce the morbidity and simultaneously slow down the transpiration of the plant water. The film has excellent film forming property, forms a transparent and soft film after being sprayed and applied, can obviously reduce the water loss of plants, does not interfere the growth or normal respiration of the plants, can adjust the closure of air holes of leaves of the plants, obstruct the invasion path of pathogenic bacteria, prevent the invasion of the pathogenic bacteria, reduce the morbidity and simultaneously slow down the water transpiration of the plants.

The further technical scheme is that the excipient is solvent, surfactant, preservative and stabilizer.

The further technical scheme is that the surfactant comprises one or a mixture of more than two of nonionic surfactant, anionic surfactant, cationic surfactant and amphoteric surfactant. The nonionic surfactant is one or a mixture of more than two of fatty alcohol polyoxyethylene ether (JFC), fatty acid polyoxyethylene ether, fatty amine polyoxyethylene ether, polyarylphenol polyoxyethylene ether, alkoxy polyoxyethylene ether, alkanolamide polyoxyethylene ether, fatty acid ester polyoxyethylene ether, castor oil polyoxyethylene ether, trisiloxane polyoxyethylene ether and alkylphenol polyoxyethylene ether (TX-10); the anionic surfactant is one or a mixture of more than two of alkyl ether carboxylate, alkylbenzene sulfonate, alkyl sulfonate, alpha-olefin sulfonate, succinate sulfonate, polyoxyethylene ether sulfate, fatty amide taurate, fatty alcohol sulfate and phosphate; the cationic surfactant is one or a mixture of more than two of ammonium salt, quaternary ammonium salt, amine salt, heterocyclic type, polymeric type and onium salt type; the amphoteric surfactant is one or a mixture of more than two of betaine type, imidazoline type, amino acid type and amine oxide type.

The further technical scheme is as follows: the raw material components comprise, by mass, 5-30% of salicylic acid gamma-aminobutyric acid derivatives, 3-20% of alginate gel, 6-12% of surfactants, 3-10% of preservatives, 1-10% of stabilizers and the balance of solvents.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid derivative solution, heating a solvent to 70-100 ℃, sequentially adding salicylic acid, gamma-aminobutyric acid and alkali metal or alkaline earth metal into the solvent, and heating and stirring at the speed of 200 revolutions per minute for 2 hours until the salicylic acid, the gamma-aminobutyric acid and the alkali metal or alkaline earth metal are completely dissolved;

s2, adding a surfactant into the solution prepared in the step S1, stirring at a constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, adding alginate jelly and a stabilizer slowly into the solution prepared in the S2, stirring at the speed of 100 revolutions per minute at the temperature of 55-60 ℃ for 30-40 minutes, adjusting the pH value to 8-10 to enable the solution to be in a colloidal suspension state, and adjusting the pH value by adding edible alkali, wherein other alkaline substances can be added for adjustment to ensure the stability of the colloidal suspension state;

s4, adding the preservative into the solution prepared in the S3, reducing the rotating speed to 60 revolutions per minute, stirring until the preservative is completely dissolved, cooling and filling.

The plant transpiration inhibitor is in a colloidal suspension shape, the particle size of oil drops of diluent of the colloidal suspension is nano-scale, the dispersion degree of active ingredients is high, the particle size is small, the wetting spreading is excellent, the film forming property is good, the plant transpiration inhibitor has good wetting spreading effect on plant leaves which are difficult to wet, and meanwhile, when the plant transpiration inhibitor is used on fruits, vegetables and flowers, residual stains can not be formed, and the quality is not influenced.

The technical scheme is that the raw material components comprise, by mass, 5-30% of salicylic acid gamma-aminobutyric acid derivatives, 3-20% of alginate gel, 6-14% of surfactants, 3-10% of preservatives, 1-10% of stabilizers and the balance of solvents.

The invention has the following advantages:

the plant transpiration inhibitor has the effect of regulating the closure of stomata of leaves of crops, has strong wetting and spreading capacity when being sprayed on the leaves of the plants, forms a synergistic interaction with algin with different action modes, improves the anti-transpiration effect, has the functions of activating the immune system and defense mechanism of the plants, forms a synergistic interaction with the algin with different action modes, and improves the protection effect on the plants.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the embodiments of the present invention will be described in detail and completely with reference to the accompanying drawings.

Thus, the following detailed description of embodiments of the invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

In addition, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.

Example 1

A plant transpiration inhibitor comprises the following raw material components: the salicylic acid-gamma-aminobutyric acid derivative is salicylic acid-gamma-aminobutyric acid potassium and algin, the algin is sodium alginate and an excipient, the excipient comprises a solvent, and the solvent is water, a surfactant, a preservative and a stabilizer. The raw material components comprise, by mass, 20% of potassium salicylate gamma-aminobutyrate, 5% of sodium alginate, 2% of JFC, 0203B 4% (0203B is a compound of various nonionic surfactants and anionic surfactants), and 5% of a preservative, wherein the preservative is sodium benzoate and 6% of a stabilizer, the stabilizer is xanthan gum powder, and the balance is water.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing 20% of salicylic acid-gamma-aminobutyric acid potassium solution, heating water to 90 ℃, sequentially adding 8% of salicylic acid, 8% of gamma-aminobutyric acid and 4% of alkali metal potassium, and heating and stirring at the speed of 200 revolutions per minute for 2 hours until the salicylic acid, the gamma-aminobutyric acid and the alkali metal potassium are completely dissolved;

s2, adding JFC 2% and 0203B 4% into the solution prepared in the step S1, stirring at constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially and slowly adding 5% of sodium alginate and 6% of xanthan gum powder into the solution prepared in the S2, stirring at the temperature of 55 ℃ at the speed of 100 revolutions per minute for 40 minutes, and adjusting the pH value to 8 to enable the solution to be in a colloidal suspension state;

s4, adding 5% of sodium benzoate into the solution prepared in the S3, reducing the rotating speed to 60 r/min, stirring until the sodium benzoate is completely dissolved, cooling and filling.

Example 2

A plant transpiration inhibitor comprises the following raw material components: the salicylic acid-gamma-aminobutyric acid derivative is salicylic acid-gamma-aminobutyric acid potassium and algin, the algin is alginic acid potassium and an excipient, the excipient comprises a solvent, and the solvent is water, a surfactant, a preservative and a stabilizer. The raw material components comprise, by mass, 22% of salicylic acid gamma-aminobutyrate potassium, 3% of potassium alginate, 2% of JFC, 0203B 4% of JFC, and 3% of preservative, wherein the preservative is sodium benzoate and 1% of stabilizer, the stabilizer is xanthan gum powder, and the balance is water.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid potassium solution 22%, heating water to 100 ℃, sequentially adding 10% of salicylic acid, 10% of gamma-aminobutyric acid and 2% of alkali metal potassium, and heating and stirring at the speed of 200 revolutions per minute for 2 hours until the salicylic acid, the gamma-aminobutyric acid and the alkali metal potassium are completely dissolved;

s2, adding JFC 2% and alkylphenol ethoxylates 4% into the solution prepared in the S1, stirring at the constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially adding 3% of potassium alginate and 1% of xanthan gum powder into the solution prepared in the S2, stirring at the temperature of 58 ℃ at the speed of 100 revolutions per minute for 30 minutes, and adjusting the pH value to 9 to enable the solution to be in a colloidal suspension state;

s4, adding 6% of sodium benzoate into the solution prepared in the S3, reducing the rotating speed to 60 r/min, stirring until the sodium benzoate is completely dissolved, cooling and filling.

Example 3

A plant transpiration inhibitor comprises the following raw material components: the salicylic acid-gamma-aminobutyric acid derivative is salicylic acid-gamma-aminobutyric acid magnesium and algin, the algin is potassium alginate and an excipient, the excipient comprises a solvent, and the solvent is water, a surfactant, a preservative and a stabilizer. The raw material components comprise, by mass, 30% of magnesium salicylate gamma-aminobutyrate, 5% of potassium alginate, 5% of JFC, 4% of alkyl ether carboxylate and 5% of preservative, the preservative is sodium benzoate and 7% of stabilizer, the stabilizer is xanthan gum powder and the balance is water.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid magnesium solution 30%, heating water to 100 ℃, sequentially adding 12% of salicylic acid, 12% of gamma-aminobutyric acid and 6% of alkaline earth metal magnesium into the water, and heating and stirring the mixture at the speed of 200 revolutions per minute for 2 hours until the mixture is completely dissolved;

s2, adding JFC 5% and alkyl ether carboxylate 4% into the solution prepared in the step S1, stirring at a constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially adding 5% of potassium alginate and 7% of xanthan gum powder into the solution prepared in the step S2, stirring at the temperature of 55 ℃ at the speed of 100 revolutions per minute for 35 minutes, and adjusting the pH value to 9 to enable the solution to be in a colloidal suspension state;

s4, adding 5% of sodium benzoate into the solution prepared in the S3, reducing the rotating speed to 60 r/min, stirring until the sodium benzoate is completely dissolved, cooling and filling.

Example 4

A plant transpiration inhibitor comprises the following raw material components: the salicylic acid-gamma-aminobutyric acid derivative is salicylic acid-gamma-aminobutyric acid calcium and algin, the algin is potassium alginate and an excipient, the excipient comprises a solvent, and the solvent is water, a surfactant, a preservative and a stabilizer. The raw material components comprise, by mass, 30% of calcium salicylate gamma-aminobutyric acid, 5% of potassium alginate, 6% of polyoxyethylene ether, 0203B 4% and 4% of a preservative, wherein the preservative is sodium benzoate and 6% of a stabilizer, the stabilizer is xanthan gum powder, and the balance is water.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid calcium solution 30%, heating water to 85 ℃, sequentially adding 11% of salicylic acid, 11% of gamma-aminobutyric acid and 8% of alkaline earth metal calcium into the water, and heating and stirring at the speed of 200 revolutions per minute for 2 hours until the calcium is completely dissolved;

s2, adding 6% of polyoxyethylene ether and 0203B 4% into the solution prepared in the step S1, stirring at the constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially adding 5% of potassium alginate and 6% of xanthan gum powder into the solution prepared in the S2, stirring at the temperature of 60 ℃ at the speed of 100 rpm for 38 minutes, and adjusting the pH value to 10 to enable the solution to be in a colloidal suspension state;

s4, adding 4% of sodium benzoate into the solution prepared in the S3, reducing the rotating speed to 60 r/min, stirring until the sodium benzoate is completely dissolved, cooling and filling.

Example 5

A plant transpiration inhibitor comprises the following raw material components: the salicylic acid-gamma-aminobutyric acid derivative is salicylic acid-gamma-aminobutyric acid potassium and algin, the algin is alginic acid potassium and an excipient, the excipient comprises a solvent, and the solvent is water, a surfactant, a preservative and a stabilizer. The raw material components comprise, by mass, 40% of salicylic acid gamma-amino potassium butyrate, 10% of potassium alginate, 10% of alkanolamide polyoxyethylene ether, 0203B 3% and 6% of a preservative, wherein the preservative is sodium benzoate and 6% of a stabilizer, the stabilizer is xanthan gum powder, and the balance is water.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid potassium solution 40%, heating water to 88 ℃, sequentially adding 15% of salicylic acid, 15% of gamma-aminobutyric acid and 10% of alkali metal potassium, and heating and stirring at the speed of 200 revolutions per minute for 2 hours until the salicylic acid, the gamma-aminobutyric acid and the alkali metal potassium are completely dissolved;

s2, adding 10% of alkanolamide polyoxyethylene ether and 0203B 3% into the solution prepared in the step S1, stirring at the constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially adding 10% of potassium alginate and 6% of xanthan gum powder into the solution prepared in the S2, stirring at the temperature of 58 ℃ at the speed of 100 rpm for 36 minutes, and adjusting the pH value to 10 to enable the solution to be in a colloidal suspension state;

s4, adding 6% of sodium benzoate into the solution prepared in the S3, reducing the rotating speed to 60 r/min, stirring until the sodium benzoate is completely dissolved, cooling and filling.

EXAMPLE six

A plant transpiration inhibitor comprises the following raw material components: the salicylic acid-gamma-aminobutyric acid derivative is salicylic acid-gamma-aminobutyric acid sodium and algin, the algin is potassium alginate and an excipient, the excipient comprises a solvent, and the solvent is water, a surfactant, a preservative and a stabilizer. The raw material components comprise, by mass, 10% of sodium salicylate gamma-aminobutyrate, 20% of potassium alginate, 5% of fatty acid ester polyoxyethylene ether, 0203B 4% and 8% of preservative, wherein the preservative is sodium benzoate and 9% of stabilizer, the stabilizer is xanthan gum powder, and the balance is water.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid sodium solution 10%, heating water to 95 ℃, sequentially adding 3% of salicylic acid, 3% of gamma-aminobutyric acid and 4% of alkali metal sodium into the water, and heating and stirring at the speed of 200 revolutions per minute for 2 hours until the salicylic acid, the gamma-aminobutyric acid and the alkali metal sodium are completely dissolved;

s2, adding 5% of fatty acid ester polyoxyethylene ether and 0203B 4% into the solution prepared in the step S1, stirring at the constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially adding 20% of potassium alginate and 9% of xanthan gum powder into the solution prepared in the step S2, stirring at the temperature of 58 ℃ at the speed of 100 rpm for 39 minutes, and adjusting the pH value to 9 to enable the solution to be in a colloidal suspension state;

s4, adding 8% of sodium benzoate into the solution prepared in the S3, reducing the rotating speed to 60 r/min, stirring until the sodium benzoate is completely dissolved, cooling and filling.

Example 7

A plant transpiration inhibitor comprises the following raw material components: the salicylic acid-gamma-aminobutyric acid derivative is salicylic acid-gamma-aminobutyric acid calcium and algin, the algin is sodium alginate and an excipient, the excipient comprises a solvent, and the solvent is water, a surfactant, a preservative and a stabilizer. The raw material components comprise, by mass, 5% of calcium salicylate gamma-aminobutyric acid, 15% of sodium alginate, 5% of fatty alcohol-polyoxyethylene ether, 5% of calcium alkylsulfonate and 6% of preservative, wherein the preservative is sodium benzoate and 8% of stabilizer, the stabilizer is xanthan gum powder, and the balance is water.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid calcium solution 5%, heating water to 75 ℃, sequentially adding 2% of salicylic acid, 2% of gamma-aminobutyric acid and 1% of alkaline earth metal calcium into the water, and heating and stirring at the speed of 200 revolutions per minute for 2 hours until the salicylic acid, the gamma-aminobutyric acid and the alkaline earth metal calcium are completely dissolved;

s2, adding 5% of fatty alcohol-polyoxyethylene ether and 5% of calcium alkyl sulfonate into the solution prepared in the step S1, stirring at a constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially adding 15% of sodium alginate and 8% of xanthan gum powder into the solution prepared in the step S2, stirring at the temperature of 56 ℃ at the speed of 100 revolutions per minute for 39 minutes, and adjusting the pH value to be 8 to enable the solution to be in a colloidal suspension state;

s4, adding 6% of sodium benzoate into the solution prepared in the S3, reducing the rotating speed to 60 r/min, stirring until the sodium benzoate is completely dissolved, cooling and filling.

Example 8

A plant transpiration inhibitor comprises the following raw material components: the salicylic acid-gamma-aminobutyric acid derivative is salicylic acid-gamma-aminobutyric acid magnesium and algin, the algin is sodium alginate and an excipient, the excipient comprises a solvent, and the solvent is water, a surfactant, a preservative and a stabilizer. The raw material components comprise, by mass, 30% of magnesium salicylate gamma-aminobutyric acid, 15% of sodium alginate, 5% of cocamidopropyl betaine, 3% of fatty alcohol polyoxyethylene ether phosphate and 4% of a preservative, wherein the preservative is sodium benzoate and 4% of a stabilizer, the stabilizer is xanthan gum powder, and the balance is water.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid magnesium solution 30%, heating water to 82 ℃, sequentially adding 10% of salicylic acid, 10% of gamma-aminobutyric acid and 10% of alkaline earth metal magnesium into the water, and heating and stirring the mixture at the speed of 200 revolutions per minute for 2 hours until the mixture is completely dissolved;

s2, adding 5% of cocamidopropyl betaine and 3% of fatty alcohol-polyoxyethylene ether phosphate into the solution prepared in the step S1, stirring at a constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially adding 15% of sodium alginate and 4% of xanthan gum powder into the solution prepared in the step S2, stirring at the temperature of 55 ℃ at the speed of 100 rpm for 40 minutes, and adjusting the pH value to 9 to enable the solution to be in a colloidal suspension state;

s4, adding 4% of sodium benzoate into the solution prepared in the S3, reducing the rotating speed to 60 r/min, stirring until the sodium benzoate is completely dissolved, cooling and filling.

Example 9

A plant transpiration inhibitor comprises the following raw material components: the salicylic acid-gamma-aminobutyric acid derivative is salicylic acid-gamma-aminobutyric acid potassium and algin, the algin is sodium alginate and an excipient, the excipient comprises a solvent, and the solvent is water, a surfactant, a preservative and a stabilizer. The raw material components comprise, by mass, 12% of salicylic acid-gamma-aminobutyrate potassium, 20% of sodium alginate, 4% of trisiloxane polyoxyethylene ether, 3% of alkylsulfonate and 6% of preservative, wherein the preservative is sodium benzoate and 4% of stabilizer, the stabilizer is xanthan gum powder, and the balance is water.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid magnesium solution of 12 percent, heating water to 85 ℃, sequentially adding 4 percent of salicylic acid, 4 percent of gamma-aminobutyric acid and 4 percent of alkali metal potassium into the water, and heating and stirring the mixture at the speed of 200 revolutions per minute for 2 hours until the mixture is completely dissolved;

s2, adding 4% of trisiloxane polyoxyethylene ether and 3% of alkyl sulfonate into the solution prepared in the step S1, stirring at a constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially adding 20% of sodium alginate and 4% of xanthan gum powder into the solution prepared in the step S2, stirring at the temperature of 58 ℃ at the speed of 100 rpm for 38 minutes, and adjusting the pH value to 8 to enable the solution to be in a colloidal suspension state;

s4, adding 6% of sodium benzoate into the solution prepared in the S3, reducing the rotating speed to 60 r/min, stirring until the sodium benzoate is completely dissolved, cooling and filling.

Example 10

A plant transpiration inhibitor comprises the following raw material components: the salicylic acid-gamma-aminobutyric acid derivative is salicylic acid-gamma-aminobutyric acid calcium and algin, the algin is potassium alginate and an excipient, the excipient comprises a solvent, and the solvent is water, a surfactant, a preservative and a stabilizer. The raw material components comprise, by mass, 22% of calcium salicylate gamma-aminobutyrate, 20% of potassium alginate, 7% of calcium alkylsulfonate, 3% of polyarylphenol polyoxyethylene ether and 6% of a preservative, wherein the preservative is sodium benzoate and 4% of a stabilizer, the stabilizer is xanthan gum powder and the balance is water.

A preparation method of a plant transpiration inhibitor comprises the following steps:

s1: preparing a salicylic acid-gamma-aminobutyric acid calcium solution 22%, heating water to 85 ℃, sequentially adding 8% of salicylic acid, 8% of gamma-aminobutyric acid and 6% of alkaline earth metal calcium into the water, and heating and stirring at the speed of 200 revolutions per minute for 2 hours until the salicylic acid, the gamma-aminobutyric acid and the alkaline earth metal calcium are completely dissolved;

s2, adding 7% of calcium alkyl sulfonate and 3% of polyarylphenol polyoxyethylene ether into the solution prepared in the step S1, stirring at the constant temperature of 60 ℃ until all the components are completely dissolved, and standing for later use;

s3, sequentially adding 20% of potassium alginate and 4% of xanthan gum powder into the solution prepared in the step S2, stirring at the temperature of 58 ℃ at the speed of 100 rpm for 37 minutes, and adjusting the pH value to 10 to enable the solution to be in a colloidal suspension state;

s4, adding 6% of sodium benzoate into the solution prepared in the S3, reducing the rotating speed to 60 r/min, stirring until the sodium benzoate is completely dissolved, cooling and filling.

Bioassay test:

1. the plant transpiration inhibitor is used for an effect test for reducing peanut transpiration.

And (3) test crops: peanuts in the course of standing in a group.

Test site: plant protection institute teaching practice base of the university of Henan agriculture (Henan Tong permit).

The test principle and method are as follows: most of the water absorbed by the plants is lost out of the body through the transpiration, only a small part of the water is supplied for the life activities of the plants, and when the transpiration is inhibited, the water absorbed by the plants is correspondingly reduced, so that the strength of the transpiration can be judged through the change of the water consumption of the plants.

50 peanuts with completely consistent physiological status were selected from each test group and were watered with an equal amount of tap water for use. The method comprises the steps of uniformly spraying an isovolumetric plant transpiration inhibitor diluted by water on the surfaces of peanut leaves in a treatment group, uniformly spraying water with equal volume on the surfaces of peanut leaves in a blank group, investigating the residual water amount after spraying for 7 days for the first time, selecting a sunny day, measuring the transpiration rate of the peanut leaves in each treatment and control group by using an LI1600 steady-state stomatometer at 12:00 noon, and measuring the residual water amount and the leaf transpiration rate again after spraying for 7 days for the second time after 10 days.

The calculation formula of the dosage of the plant transpiration inhibitor and the water saving rate of each test group is as follows:

water saving rate [% of blank group water consumption-treatment group water consumption ]/[ 100 ] of blank group water consumption

Test groups: spraying the plant transpiration inhibitor prepared in each embodiment

Standard control group: spraying and spraying commercial plant transpiration inhibitor lumbricus with the same volume and common concentration

Blank group: spraying water with the same volume as the experimental group

Test results

TABLE 1 statistical effect of plant transpiration inhibitor for decreasing peanut transpiration

Note: the dilution factor in the test formulation examples is the effective content of the formulation x2000

As can be seen from Table 1, the water consumption and the transpiration rate of the plant transpiration inhibitor treatment groups sprayed in the examples of the invention are obviously lower than those of the single dose group and the blank group of sodium (potassium) alginate and salicylic acid, sodium (potassium) alginate and potassium gamma-aminobutyrate, and the water consumption and the transpiration rate of the plant transpiration inhibitor treatment groups of the examples 6, 7, 8 and 9 are obviously lower than those of the plant transpiration inhibitor of the control group of the lumbricus. The water saving rate of each example of the treatment group is obviously higher than that of a single dose group and a blank group of sodium (potassium) alginate and salicylic acid, sodium (potassium) alginate and potassium gamma-aminobutyrate, and the water saving rate of the plant transpiration inhibitor treatment groups of the examples 6, 7, 8 and 9 is obviously higher than that of a plant transpiration inhibitor lumbricus of a control group.

The water-saving effect of the plant transpiration inhibitor of the salicylic acid-gamma-aminobutyric acid derivative-sodium (potassium) alginate compound treatment group on the peanuts is obvious.

2. The plant transpiration inhibitor is used for an effect test for reducing the transpiration of apple trees.

Test work: 4-year-old apple tree

Test site: teaching practice base of gardening academy of Henan university of agriculture (Henan Lingbao)

The test method comprises the following steps: spraying plant transpiration inhibitor for 4-year-old apple trees.

Spraying clear water as a control, then trimming part of the branches with leaves, and airing for 24 hours and 48 hours respectively.

And randomly selecting 10 seedlings with consistent growth vigor for each treatment, and uniformly spraying. After one day of treatment, part of the branches with leaves are trimmed and aired. The daily average air temperature is 20 ℃, the daily average ground temperature is 15 ℃ and the average relative humidity is 40% during airing. And (5) surveying and recording the water loss rate and drought resistance efficiency of each treated seedling.

The water loss rate of the nursery stock: randomly extracting 5 plants from each 10 treated plants, uniformly spraying a plant transpiration inhibitor on the whole plant according to experimental design for 24 hours, then pruning partial branches with leaves, and respectively weighing the fresh weight of the pruned branches with leaves and the fresh weight of stems by using a balance. Placing the fresh and heavy seedling stems and leaves outdoors, airing according to the experimental design, and weighing after the designed treatment time. The water loss rate of the apple seedlings can be calculated according to the fresh weight before airing and the weight after airing. The test results are shown in table 2.

Water loss rate%

Seedling withering rate: the test is arranged in a shallow mountain area without irrigation condition under drought and rain, the treated apple trees are managed according to a normal method, green unfolded leaves are in a normal growth standard, the micro-roll water-deficient growth stagnation and withering of the leaves are in a drought standard, after 30 days and 60 days of treatment, the normal growth plant number of the apple trees treated in different ways in the whole test area is investigated, and the dry leaf withering rate of each treatment is calculated. Thus, the water-saving effect of spraying the anti-transpiration inhibitor is proved.

Test results

TABLE 2 statistical table of experimental effect of plant transpiration inhibitor for reducing transpiration of apple trees

Note: the dilution factor in the test formulation examples is the effective content of the formulation x2000

As can be seen from the results in Table 2, when the sodium (potassium) alginate, the salicylic acid and the r-aminobutyric acid derivative of the invention are sprayed, the water loss rate of branches and leaves of the plant transpiration inhibitor in the sodium (potassium) alginate compound treatment example is obviously lower than that of the sodium (potassium) alginate, the salicylic acid, the 50% magnesium phosphite single agent group and the blank group, the withering rate of apple trees in the plant transpiration inhibitor treatment group in the example is obviously lower than that of the salicylic acid sodium (potassium) alginate, the 50% magnesium phosphite single agent group and the blank group, which shows that the anti-transpiration effect of each compound treatment group is relatively remarkable, the plant transpiration inhibitor containing the salicylic acid r-aminobutyric acid derivative sodium (potassium) alginate in the example of the invention plays a positive promotion role in the aspects of inhibiting the water loss of the seedlings, increasing the water content of the stems and leaves of the apple seedlings, prolonging the drought-resistant life time of the apple seedlings, and is beneficial to the drought-resistant growth of the apple trees in hilly mountain areas, has production and use values.

3. The plant transpiration inhibitor is used for the field efficacy test of peanut root rot.

Test crops and diseases: root rot of peanut

Test work: peanut treatment time: in the course standing period;

diseases of test crops: root rot of peanut.

Test site: plant protection college teaching practice base of Henan university of agriculture (Henan Tong permit)

The test method comprises the following steps: the treatment cells were randomly arranged and repeated 4 times for each treatment cell area (2x6.6) of 13.2m 2. Every 30 days of treatment, root irrigation is carried out once with water by 400mL, medicine irrigation is continuously carried out for 2 times, sampling is carried out at five random points in each cell, 6m double-row investigation is carried out at fixed points for disease incidence of peanuts treated, investigation is carried out by taking a plant as a unit, the total number of investigated plants and the number of diseased plants of peanuts are recorded, the disease incidence and the prevention effect are calculated, and the calculation formula of the disease incidence and the prevention effect is as follows. And random five-point sampling is adopted in each cell, 2m of samples are taken at each point, the agronomic character indexes of the peanuts in each treatment are investigated in two lines, the plant height, the root length and the fresh weight of the whole peanut are investigated, and the plant height, the root length and the fresh weight difference of the peanuts in each treatment are compared. The test results are shown in Table 3.

Incidence [% incidence of disease/total number of investigated plants [% 100 [ ]

The control effect is (control area disease rate-treatment area disease rate)/control area disease rate is 100%.

Test results

TABLE 3 statistical table of the test results of salicylic acid, gamma-aminobutyric acid derivatives, sodium (potassium) alginate on flower root rot

Note: the dilution factor in the test formulation examples is the effective content of the formulation x2000

The results in table 3 show that when the salicylic acid, r-aminobutyric acid derivative and sodium (potassium) alginate compound treatment group in the embodiment of the invention is sprayed, the incidence rate of peanut root rot is obviously lower than that of a sodium (potassium) alginate, salicylic acid and sodium (potassium) alginate r-potassium aminobutyrate single dose group and a blank group, and the prevention effect of each treatment in the compound embodiment on the peanut root rot is obviously higher than that of the sodium (potassium) alginate, the salicylic acid and the sodium (potassium) alginate r-potassium aminobutyrate single dose; the salicylic acid-r-aminobutyric acid derivative-sodium (potassium) brown alginate compound treatment group in the embodiment has a good prevention effect on peanut root rot.

The determination results of the agronomic characters and the yield indexes of the peanuts show that the plant height, the root length and the fresh weight of the peanut plants in the salicylic acid, r-aminobutyric acid derivatives and sodium (potassium) alginate compound treatment group are obviously higher than those of sodium (potassium) alginate, salicylic acid and sodium (potassium) alginate r-aminobutyrate single dose and blank group, and the results show that the salicylic acid, r-aminobutyric acid derivatives and sodium (potassium) alginate compound treatment group in the embodiment has obvious growth promotion effect on the growth of the peanuts. In the test process, the physiological state of each treated peanut is normal, and the abnormal shape does not appear.

From the test results of the application examples, the salicylic acid r-aminobutyric acid derivative sodium (potassium) alginate has excellent transpiration resistance and water saving effects on peanuts and apple trees, and is beneficial to improving the drought-resistant growth function of apple saplings.

The salicylic acid, the r-aminobutyric acid derivative and the sodium (potassium) alginate have excellent prevention effect on flower root rot and have good growth promotion effect on peanut seedlings. Meanwhile, in the test process, the salicylic acid-r-aminobutyric acid derivative-sodium (potassium) brown alginate has a promoting function on the drought resistance and safe growth of peanuts and apple trees, and is a plant transpiration inhibitor which is worth to be popularized and used.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some of the features described in the embodiments and/or modifications thereof can be made without departing from the spirit and scope of the invention.