阅读说明：本技术 一种复配植物提取物及其制备方法和应用 (Compound plant extract and preparation method and application thereof ) 是由 王强 石爱民 郭瑞 胡晖 刘红芝 刘丽 郭芹 张雨 焦博 张金闯 于 2021-07-21 设计创作，主要内容包括：本发明涉及植物提取物领域,尤其涉及一种复配植物提取物及其制备方法和应用。所述复配植物提取物的制备方法包括：以花生茎叶、酸枣仁和桑椹干为原料,进行水提取；其中,所述花生茎叶、所述酸枣仁和所述桑椹干的重量比为5～20：1～10：1～10。本发明发现花生茎叶、酸枣仁和桑椹干具有良好的协同作用,以三者为原料可制得具有很好的改善睡眠和抗焦虑功能的配植物提取物。本发明通过对提取方法的优化,使复配植物提取物中充分保留了多酚、多糖类化合物、微量元素以及具有功能作用的黄酮类促睡眠成分,使其具有显著的改善睡眠和抗焦虑的功能。(The invention relates to the field of plant extracts, in particular to a compound plant extract and a preparation method and application thereof. The preparation method of the compound plant extract comprises the following steps: extracting peanut stem and leaf, spine date seed and dried mulberry serving as raw materials with water; wherein the weight ratio of the peanut stems and leaves, the spine date seed and the dried mulberry is 5-20: 1-10: 1 to 10. The invention finds that the peanut stem leaves, the spina date seeds and the dried mulberries have good synergistic effect, and the prepared plant extract with good sleep improving and anxiety resisting functions can be prepared by taking the peanut stem leaves, the spina date seeds and the dried mulberries as raw materials. According to the invention, through optimization of the extraction method, polyphenol, polysaccharide compounds, trace elements and flavonoid sleep-promoting components with functional effects are fully reserved in the compound plant extract, so that the compound plant extract has the functions of obviously improving sleep and resisting anxiety.)

1. A preparation method of a compound plant extract is characterized by comprising the following steps: extracting peanut stem and leaf, spine date seed and dried mulberry serving as raw materials with water;

wherein the weight ratio of the peanut stems and leaves, the spine date seed and the dried mulberry is 5-20: 1-10: 1 to 10.

2. The preparation method of claim 1, wherein the water extraction has a ratio of 1: 10 to 20 (g/mL).

3. The method according to claim 1 or 2, wherein the water extraction is performed 1 to 3 times at 90 to 100 ℃ for 1 to 4 hours each time.

4. The production method according to any one of claims 1 to 3, further comprising: and concentrating the crude extract obtained after the water extraction to 1/2-1/3 of the original volume.

5. The method of claim 1, comprising the steps of:

(1) mixing peanut stem leaves, wild jujube kernels and dried mulberry according to a weight ratio of 5-20: 1-10: 1-10 to obtain a mixture;

(2) mixing the mixture with water according to a material-water ratio of 1: 10-20 (g/mL), and performing reflux extraction at 90-100 ℃ for 1-3 times, wherein each time lasts for 1-4 hours to obtain a crude extract;

(3) and (3) carrying out low-temperature vacuum concentration on the crude extract, concentrating to 1/2-1/3 of the original volume, and then carrying out vacuum freeze drying.

6. The preparation method of claim 5, wherein the peanut stems and leaves are preliminarily subjected to superfine grinding to 100-400 meshes;

and/or the spina date seeds are subjected to superfine grinding in advance to be 100-400 meshes.

7. A compound plant extract is characterized by being prepared by the preparation method of any one of claims 1 to 6;

preferably, in the compound plant extract, the content of 4', 7-di-O-methyl naringenin is not lower than 114.75mg/kg, and the content of 2' -O-methyl isoliquiritigenin is not lower than 24.4093 mg/kg.

8. The use of the compounded plant extract of claim 7 in the preparation of medicaments, foods or food additives;

preferably, the medicine, food or food additive has a function of improving sleep and/or anxiolytic.

9. The composition is characterized by comprising the following components in parts by weight: 25-50 parts of the compound plant extract of claim 7, 25-50 parts of maltodextrin, 50-75 parts of sucrose powder and 0.05-0.1 part of b-cyclodextrin;

preferably, the composition is in the form of granules.

10. A method of preparing the composition of claim 9, comprising: mixing the components, and performing dry granulation;

preferably, the particle size of the composition is 40-20 meshes.

Technical Field

The invention relates to the field of plant extracts, in particular to a compound plant extract and a preparation method and application thereof.

Background

The sleep time of a life of a person is about more than 30 percent, the sleep is closely related to the physiology and the psychology of the body, activities such as learning, memory, social behaviors and the like are influenced due to insufficient sleep or poor sleep quality, and complications such as obesity, heart disease, stroke and the like are caused in the past. Notably, sleep disorders are associated with a number of neurological disorders, cardiac disorders, and the like.

Although sleep-promoting drugs such as barbiturates and benzodiazepines can prolong sleep time, adverse side effects such as addiction dependence, withdrawal response and ataxia are easy to generate, and no ideal hypnotic drug with the advantages of high decomposition rate, no lag, no dependence and the like is found in the market at present.

In view of this, the invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a compound plant extract which has good functions of improving sleep and resisting anxiety. The invention also aims to provide a preparation method and application of the compound plant extract.

The invention aims to develop a compound plant extract with the functions of improving sleep and resisting anxiety, and develops a process for producing the compound plant extract with the functions of improving sleep and resisting anxiety by taking compound plants as raw materials according to the characteristics of the raw materials and the composition characteristics of the extract. The composition of the product of the compound plant ingredients after water extraction is quite complex, and the product contains a large number of components with unknown functions, while the components with specific functions may contain a plurality of components with different molecular weights, and are difficult to distinguish through certain common characteristics. These all pose great difficulties in developing plant extracts with specific functions. In the research and development process, a plurality of compound plant raw materials are tried, and a plurality of compound plant raw materials are difficult to obtain components with the functions of improving sleep and resisting anxiety. After a great deal of attempts, the invention finally determines the process for obtaining the compound plant extract with better sleep improving and anxiolytic functions by taking peanut stems and leaves, spina date seeds and dried mulberries as raw materials.

Specifically, the invention firstly provides a preparation method of a compound plant extract, which comprises the following steps: extracting peanut stem and leaf, spine date seed and dried mulberry serving as raw materials with water;

wherein the weight ratio of the peanut stems and leaves, the spine date seed and the dried mulberry is 5-20: 1-10: 1 to 10.

The invention discovers that the contents of the components with the functions of improving sleep and resisting anxiety in the product can be obviously increased by taking the peanut stem leaves, the wild jujube kernels and the dried mulberries in the proportion as raw materials and extracting the raw materials with water, so that the functions of improving sleep and resisting anxiety of the product are greatly improved.

Preferably, the water extraction has a feed-water ratio of 1: 10 to 20 (g/mL).

Preferably, the water extraction is carried out for 1-3 times at 90-100 ℃, and each time lasts for 1-4 hours.

In the invention, the mixed raw materials are extracted by water under the conditions, which is beneficial to the dissolution of components with the functions of improving sleep and resisting anxiety.

As a preferred technical scheme, the ratio of material to water in the water extraction is 1: 10(g/mL), the water extraction was performed 2 times at 95 ℃ for 2h each time.

Preferably, the preparation method further comprises: and concentrating the crude extract obtained after the water extraction to 1/2-1/3 of the original volume.

As a better technical scheme of the invention, the preparation method comprises the following steps:

(1) mixing peanut stem leaves, wild jujube kernels and dried mulberry according to a weight ratio of 5-20: 1-10: 1-10 to obtain a mixture;

(2) mixing the mixture with water according to a material-water ratio of 1: 10-20 (g/mL), and performing reflux extraction at 90-100 ℃ for 1-3 times, wherein each time lasts for 1-4 hours to obtain a crude extract;

(3) and (3) carrying out low-temperature vacuum concentration on the crude extract, concentrating to 1/2-1/3 of the original volume, and then carrying out vacuum freeze drying.

The compound plant extract obtained by the method has good functions of improving sleep and reducing anxiety level rise caused by sleep deprivation.

Further, the peanut stems and leaves are subjected to superfine grinding in advance to be 100-400 meshes;

further, the spina date seeds are subjected to superfine grinding in advance to be 100-400 meshes;

the peanut stem leaves and the spina date seeds are subjected to superfine grinding in advance to 100-400 meshes, so that the efficiency of subsequent water extraction is improved, and functional components are dissolved out quickly.

In a specific embodiment, peanut stems and leaves which are produced in Changji area of Xinjiang and after pod maturity and have no pesticide, mildew and disease and insect pests are adopted, air showering is carried out to remove impurities, the peanut stems and leaves are naturally dried in the sun, then the cut peanut stems and leaves are cut and crushed, and the peanut stems and leaves are further subjected to superfine crushing to 100-400 meshes for later use; adopting black mulberry fruits produced in Xinjiang, and naturally drying the black mulberry fruits for later use; the wild jujube seeds produced in Xinjiang are adopted, and are subjected to superfine grinding to 100-400 meshes after being fried for later use.

The compound plant extract extracted by the method has high content of required functional components. After quantitative analysis of the compound plant extract by a high-sensitivity liquid chromatograph-mass spectrometer, the content of the 4', 7-di-O-methyl naringenin is not lower than 114.75mg/kg, and the content of the 2' -O-methyl isoliquiritigenin is not lower than 24.4093 mg/kg.

The invention also provides a compound plant extract, which is prepared by the preparation method.

According to the invention, a great deal of researches are carried out on traditional Chinese medicine components with the functions of improving sleep and resisting anxiety, and during the researches, the extracts of peanut stem leaves, spina date seeds and mulberry fruits have various active substances which can improve sleep and resist anxiety.

Wherein, the peanut stems and leaves have the function of treating insomnia in the traditional Chinese medicine record; the characteristic compounds in the spina date seed, such as spina date seed saponin, fatty oil, alkaloid, flavonoid and the like, have the effects of calming the heart, soothing the nerves, nourishing the liver, tranquilizing, reducing blood pressure and the like; modern medical research shows that mulberry has the functions of eliminating in-vitro active oxygen free radicals, regulating organism immunity, reducing blood fat, relieving neurasthenia, improving insomnia and the like, and especially has more obvious effect compared with white mulberry.

The invention fully utilizes peanut stem leaves, spina date seeds and dried mulberries, and three plant extracts cooperate to improve sleep and resist anxiety; meanwhile, the compound plant extract has high nutritive value, good product quality stability and high safety.

Preferably, in the compound plant extract, the content of 4', 7-di-O-methyl naringenin is not less than 114.75mg/kg, and the content of 2' -O-methyl isoliquiritigenin is not less than 24.4093 mg/kg.

The invention also provides application of the compound plant extract in preparation of medicines, foods or food additives.

Preferably, the medicine, food or food additive has a function of improving sleep and/or an anxiolytic.

The invention provides a composition, which comprises the following components in parts by weight: 25-50 parts of compound plant extract, 25-50 parts of maltodextrin, 50-75 parts of sucrose powder and 0.05-0.1 part of b-cyclodextrin.

Preferably, the composition is in the form of granules.

The composition provided by the invention has good mouthfeel and unique flavor, the main components of the composition are all derived from food, the safety is high, the composition has the functions of improving sleep and resisting anxiety, the special requirements of people on recovering sleep and eliminating anxiety as soon as possible in states of staying up all night, lacking perception and the like can be met, and the composition has high practical significance and commercial value.

The invention also provides a preparation method of the composition, which comprises the following steps: the components are mixed and then are granulated by a dry method.

Preferably, the particle size of the composition is 40-20 meshes.

Compared with the prior art, the invention has the beneficial effects that:

(1) the invention finds that the peanut stem leaves, the spina date seeds and the dried mulberries have good synergistic effect, and the prepared plant extract with good sleep improving and anxiety resisting functions can be prepared by taking the peanut stem leaves, the spina date seeds and the dried mulberries as raw materials.

(2) According to the invention, through optimization of the extraction method, polyphenol, polysaccharide compounds, trace elements and flavonoid sleep-promoting components with functional effects are fully reserved in the compound plant extract, so that the compound plant extract has the functions of obviously improving sleep and resisting anxiety.

(3) The compound plant extract disclosed by the invention has the functions of improving anxiety mood and anxiety-like behaviors caused by sleep deprivation while ensuring the function of improving sleep.

(4) The composition granules containing the compound plant extract provided by the invention have the functions of improving sleep and resisting anxiety, have excellent taste and special flavor of peanut stems and leaves on the premise of not adding any pigment and essence, and are stable in quality, long in storage and convenient to take.

Drawings

Fig. 1(a) is the duration of quick eye movement sleep of a mouse taking the compound plant extract of the invention, fig. 1(B) is the duration of slow wave sleep of a mouse taking the compound plant extract of the invention, and fig. 1(C) is the duration of total sleep of a mouse taking the compound plant extract of the invention.

Fig. 2 shows the density of brain waves in slow wave sleep of mice taking the compounded plant extract of the present invention.

FIG. 3 is behavioral data in the high price plus maze of mice subjected to sleep deprivation administered the compounded plant extracts of the present invention; fig. 3(a) is a schematic diagram of the number of times of arm crossing, and fig. 3(B) is a schematic diagram of the arm-opening stay time.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or instruments used are conventional products available from regular distributors, not indicated by the manufacturer.

Example 1

The embodiment provides a compound plant extract, and a preparation method thereof comprises the following steps:

(1) adopting peanut stems and leaves (fresh peanut stems and leaves between 1 and 2 months after peanut sprouting) which are produced in Changji area of Xinjiang and after pod maturity and have no pesticide, mildew and plant diseases and insect pests, drying for 2 hours at 65 ℃ to reduce the water content to below 1 percent, and carrying out ultramicro crushing to 100-400 meshes for later use; adopting black mulberry fruits produced in Xinjiang, and naturally drying the black mulberry fruits for later use; adopting spina date seeds produced in Xinjiang, and micronizing the fried spina date seeds to 100-400 meshes for later use;

(2) mixing peanut stem and leaf, spine date seed and dried mulberry according to a weight ratio of 20: 10: 10, mixing to obtain a mixture;

(3) mixing the mixture with water according to a material-water ratio of 1: 10(g/mL), and extracting under reflux at 95 ℃ for 2 times, each time for 2 hours, to obtain a crude extract;

(4) and (3) carrying out low-temperature vacuum concentration on the crude extract, concentrating to 1/2-1/3 of the original volume, and then carrying out vacuum freeze drying.

Example 2

The embodiment provides a compound plant extract, and a preparation method thereof comprises the following steps:

(1) drying the peanut stems and leaves (fresh peanut stems and leaves after pod ripening) which are produced in Changji area of Xinjiang and are free of pesticide, mildew and insect pests after the pod ripening period at 65 ℃ for 2 hours to reduce the water content to below 1%, and carrying out ultramicro crushing to 100-400 meshes for later use; adopting black mulberry fruits produced in Xinjiang, and naturally drying the black mulberry fruits for later use; adopting spina date seeds produced in Xinjiang, and micronizing the fried spina date seeds to 100-400 meshes for later use;

(2) mixing peanut stem and leaf, spine date seed and dried mulberry according to a weight ratio of 20: 10: 10, mixing to obtain a mixture;

(3) mixing the mixture with water according to a material-water ratio of 1: 10(g/mL), and extracting under reflux at 95 ℃ for 2 times, each time for 2 hours, to obtain a crude extract;

(4) and (3) carrying out low-temperature vacuum concentration on the crude extract, concentrating to 1/2-1/3 of the original volume, and then carrying out vacuum freeze drying.

Example 3

The embodiment provides a compound plant extract, and a preparation method thereof comprises the following steps:

(1) drying the peanut stems and leaves (fresh peanut stems and leaves after pod ripening) which are produced in Changji area of Xinjiang and are free of pesticide, mildew and insect pests after the pod ripening period at 65 ℃ for 2 hours to reduce the water content to below 1%, and carrying out ultramicro crushing to 100-400 meshes for later use; adopting black mulberry fruits produced in Xinjiang, and naturally drying the black mulberry fruits for later use; adopting spina date seeds produced in Xinjiang, and micronizing the fried spina date seeds to 100-400 meshes for later use;

(2) mixing peanut stem and leaf, spine date seed and dried mulberry according to the weight ratio of 10: 10: 10, mixing to obtain a mixture;

(3) mixing the mixture with water according to a material-water ratio of 1: 10(g/mL), and extracting under reflux at 95 ℃ for 2 times, each time for 2 hours, to obtain a crude extract;

(4) and (3) carrying out low-temperature vacuum concentration on the crude extract, concentrating to 1/2-1/3 of the original volume, and then carrying out vacuum freeze drying.

Example 4

The embodiment provides a compound plant extract, and a preparation method thereof comprises the following steps:

(1) drying the peanut stems and leaves (fresh peanut stems and leaves after pod ripening) which are produced in Changji area of Xinjiang and are free of pesticide, mildew and insect pests after the pod ripening period at 65 ℃ for 2 hours to reduce the water content to below 1%, and carrying out ultramicro crushing to 100-400 meshes for later use; adopting black mulberry fruits produced in Xinjiang, and naturally drying the black mulberry fruits for later use; adopting spina date seeds produced in Xinjiang, and micronizing the fried spina date seeds to 100-400 meshes for later use;

(2) mixing peanut stem leaves, spina date seeds and dried mulberry according to a weight ratio of 5: 10: 10, mixing to obtain a mixture;

(3) mixing the mixture with water according to a material-water ratio of 1: 10(g/mL), and extracting under reflux at 95 ℃ for 2 times, each time for 2 hours, to obtain a crude extract;

(4) and (3) carrying out low-temperature vacuum concentration on the crude extract, concentrating to 1/2-1/3 of the original volume, and then carrying out vacuum freeze drying.

Example 5

The embodiment provides a compound plant extract, and a preparation method thereof comprises the following steps:

(1) drying the peanut stems and leaves (fresh peanut stems and leaves after pod ripening) which are produced in Changji area of Xinjiang and are free of pesticide, mildew and insect pests after the pod ripening period at 65 ℃ for 2 hours to reduce the water content to below 1%, and carrying out ultramicro crushing to 100-400 meshes for later use; adopting black mulberry fruits produced in Xinjiang, and naturally drying the black mulberry fruits for later use; adopting spina date seeds produced in Xinjiang, and micronizing the fried spina date seeds to 100-400 meshes for later use;

(2) mixing peanut stem and leaf, spine date seed and dried mulberry according to the weight ratio of 10: 10: 5, mixing to obtain a mixture;

(3) mixing the mixture with water according to a material-water ratio of 1: 10(g/mL), and extracting under reflux at 95 ℃ for 2 times, each time for 2 hours, to obtain a crude extract;

(4) and (3) carrying out low-temperature vacuum concentration on the crude extract, concentrating to 1/2-1/3 of the original volume, and then carrying out vacuum freeze drying.

Example 6

The embodiment provides a compound plant extract, and a preparation method thereof comprises the following steps:

(1) drying the peanut stems and leaves (fresh peanut stems and leaves after pod ripening) which are produced in Changji area of Xinjiang and are free of pesticide, mildew and insect pests after the pod ripening period at 65 ℃ for 2 hours to reduce the water content to below 1%, and carrying out ultramicro crushing to 100-400 meshes for later use; adopting black mulberry fruits produced in Xinjiang, and naturally drying the black mulberry fruits for later use; adopting spina date seeds produced in Xinjiang, and micronizing the fried spina date seeds to 100-400 meshes for later use;

(2) mixing peanut stem and leaf, spine date seed and dried mulberry according to the weight ratio of 10: 5: 10, mixing to obtain a mixture;

(3) mixing the mixture with water according to a material-water ratio of 1: 10(g/mL), and extracting under reflux at 95 ℃ for 2 times, each time for 2 hours, to obtain a crude extract;

(4) and (3) carrying out low-temperature vacuum concentration on the crude extract, concentrating to 1/2-1/3 of the original volume, and then carrying out vacuum freeze drying.

Example 7

The embodiment provides a composition, which comprises the following components in parts by weight: 25 parts of compound plant extract of example 1, 25 parts of maltodextrin, 50 parts of sucrose powder and 0.05 part of b-cyclodextrin;

the composition is in the form of granule.

This example also provides a method for preparing the composition, comprising the steps of:

(1) mixing the components by using a turnover drum mixer for 2 minutes continuously to obtain a mixture;

(2) and (3) performing dry granulation, pressing and crushing the mixture, and screening to obtain the mixture with the particle size of 20-40 meshes.

Example 8

The embodiment provides a composition, which comprises the following components in parts by weight: 25 parts of compound plant extract of example 2, 25 parts of maltodextrin, 50 parts of sucrose powder and 0.05 part of b-cyclodextrin;

the composition is in the form of granule.

This example also provides a method for preparing the composition, comprising the steps of:

(1) mixing the components by using a turnover drum mixer for 2 minutes continuously to obtain a mixture;

(2) and (3) performing dry granulation, pressing and crushing the mixture, and screening to obtain the mixture with the particle size of 20-40 meshes.

Example 9

The embodiment provides a composition, which comprises the following components in parts by weight: 25 parts of compound plant extract of example 3, 25 parts of maltodextrin, 50 parts of sucrose powder and 0.05 part of b-cyclodextrin;

the composition is in the form of granule.

This example also provides a method for preparing the composition, comprising the steps of:

(1) mixing the components by using a turnover drum mixer for 2 minutes continuously to obtain a mixture;

(2) and (3) performing dry granulation, pressing and crushing the mixture, and screening to obtain the mixture with the particle size of 20-40 meshes.

Example 10

The embodiment provides a composition, which comprises the following components in parts by weight: 25 parts of compound plant extract of example 4, 25 parts of maltodextrin, 50 parts of sucrose powder and 0.05 part of b-cyclodextrin;

the composition is in the form of granule.

This example also provides a method for preparing the composition, comprising the steps of:

(1) mixing the components by using a turnover drum mixer for 2 minutes continuously to obtain a mixture;

(2) and (3) performing dry granulation, pressing and crushing the mixture, and screening to obtain the mixture with the particle size of 20-40 meshes.

Example 11

The embodiment provides a composition, which comprises the following components in parts by weight: 25 parts of compound plant extract of example 5, 25 parts of maltodextrin, 50 parts of sucrose powder and 0.05 part of b-cyclodextrin;

the composition is in the form of granule.

This example also provides a method for preparing the composition, comprising the steps of:

(1) mixing the components by using a turnover drum mixer for 2 minutes continuously to obtain a mixture;

(2) and (3) performing dry granulation, pressing and crushing the mixture, and screening to obtain the mixture with the particle size of 20-40 meshes.

Example 12

The embodiment provides a composition, which comprises the following components in parts by weight: 25 parts of compound plant extract of example 6, 25 parts of maltodextrin, 50 parts of sucrose powder and 0.05 part of b-cyclodextrin;

the composition is in the form of granule.

This example also provides a method for preparing the composition, comprising the steps of:

(1) mixing the components by using a turnover drum mixer for 2 minutes continuously to obtain a mixture;

(2) and (3) performing dry granulation, pressing and crushing the mixture, and screening to obtain the mixture with the particle size of 20-40 meshes.

Test example 1 Effect of Compound plant extract on sleep time of mouse

1. Materials and methods

1.1 Experimental materials

Experimental animals: a male C57BL/6N mouse with the age of 6-8 weeks is selected as a study object, after the study object is fed adaptively for one week, the study object is randomly divided into 3 groups (8 in each group) of a control group, a compound extract group and a positive control group, and after the study object is continuously gavaged for 15 days, brain waves and myoelectric waves of the mouse are monitored, and the sleep quantity is counted.

Reagent: diazepam (D-907, CAS: 439-14-5), sodium carboxymethyl cellulose (C5013-500G, CAS: 9004-32-4), 2,2, 2-tribromoethanol (T48402, CAS: 75-80-9) from Sigma-Aldrich, USA, ceftriaxone sodium (C129519, CAS: 104376-79-6) from Shanghai Arlatin Biotech, Inc. (China, Shanghai); glass ionostomin was purchased from shanghai rongxiang dental materials ltd (china, shanghai); depilatory cream was purchased from rituximab limited (china, beijing).

The instrument comprises the following steps: mouse brain stereotaxic instrument, PowerLab multi-guide physiological recorder, multi-channel universal wheel, surgical instrument, etc.

1.2 Experimental methods

The experimental animal model was designed as follows: three groups of mice were gavage continuously for 15 days, the control group mice were gavage with 0.5% sodium carboxymethylcellulose aqueous solution, the compound extract group was gavage with 500mg/kg BW of the compound plant extract of example 2 dissolved in 0.5% sodium carboxymethylcellulose aqueous solution, and the positive control group was gavage with 5mg/kg BW of diazepam dissolved in 0.5% sodium carboxymethylcellulose aqueous solution.

1.3 sample index determination

After the gavage for 15 days, a PowerLab multi-lead physiological recorder is used for collecting brain waves and myoelectric waves of each mouse, Sleepsign software is used for analyzing data, and the data are divided according to the following rules: delta waves (0.65-4 Hz) in brain waves in a slow wave sleep period are main waves, the waveform amplitude is large, the myoelectricity fluctuation is small, and the brain waves are in a quite state; theta waves (6-10 Hz) in brain waves of Rapid Eye Movement Sleep (REMS) are main waves, the amplitude of the waves is small, and myoelectricity is in the most quiet state; in the WAKE period (WAKE), the brain wave amplitude is small, the muscle wave amplitude is large, and the device is in an active state. And counting the quick eye-moving sleep time length, the slow wave sleep time length and the total sleep time length of the mouse.

One-Way ANOVA analysis was performed using GraphPad 6, and multiple comparisons were performed using the Turkey method. p < 0.05 was considered statistically different.

2. Results of the experiment

2.1 Effect of Compound plant extract on the duration of quick-acting eye sleep, the duration of slow-wave sleep and the total sleep duration of mice

Measuring the fast eye-moving sleep time, the slow wave sleep time and the total sleep time of the mouse in the experimental process, wherein the results are shown in figure 1; from fig. 1(a), it can be seen that the compound plant extract of example 2 can significantly improve the quick-acting eye sleep time of C57BL/6N mice, while the positive drug diazepam has no significant effect on the quick-acting eye sleep. It can also be seen from fig. 1(B) and (C) that the slow wave sleep duration and the total sleep duration of the mice are also significantly prolonged, indicating that the compounded plant extract of example 2 is better than the positive drug diazepam in prolonging sleep time.

2.2 Effect of Compound plant extract on Density of brain waves in Slow wave sleep

Measuring brain waves of each wave band in slow wave sleep of the mouse in the experimental process, wherein the result is shown in figure 2; as can be seen from fig. 2, the compound plant extract of example 2 does not affect the distribution of brain waves of various frequencies in slow wave sleep, and does not change the sleep structure. The compound plant extract in the example 2 does not change the sleep quality, does not influence the sleep structure of the mouse in a natural state, and has low toxic and side effects.

Experimental example 2 Effect of Compound plant extract on anxiety level in mice after sleep deprivation

1. Materials and methods

1.1 Experimental materials

The experimental animals, reagents, instruments and the like were the same as those in test example 1.

1.2 Experimental methods

The experimental animal model was designed as follows: three groups of mice were continuously sleep deprived for 15 days (deprivation for 20 hours per day, recovery for 4 hours), and simultaneously were continuously gavaged for 15 days, the control group mice were gavaged with 0.5% sodium carboxymethylcellulose aqueous solution, the compound plant extract of example 2, in which the compound group was gavaged dissolved in 0.5% sodium carboxymethylcellulose aqueous solution, 500mg/kg BW, and the positive control group was gavaged with diazepam, in which the compound group was dissolved in 0.5% sodium carboxymethylcellulose aqueous solution, 5mg/kg BW.

1.3 sample index determination

Before sleep deprivation begins, after sleep deprivation and gastric lavage are carried out simultaneously for 15 days, a PowerLab multi-conduction physiological recorder is used for collecting brain waves and myoelectric waves of each mouse, and Sleepsign software is used for analyzing data. The data analysis rules and methods were the same as in test example 1.

2. Results of the experiment

2.1 Effect of Compound plant extracts on anxiety levels in mice after sleep deprivation

The anxiety level of the mice after sleep deprivation is measured by using the elevated plus maze in the experimental process, and the result is shown in figure 3; as can be seen from FIG. 3(A), there was no difference in the number of times that the mice in each treatment group entered the open arm within 10 min; as can be seen from fig. 3(B), the residence time in the open arm of the mice in the sleep-deprived group after 15 days of sleep deprivation was significantly reduced compared to the control group without sleep deprivation, whereas the residence time in the open arm of the mice in the combination group was significantly increased compared to the control group after 15 days of gastric lavage, whereas the residence time in the open arm of the mice in the positive control group administered with diazepam was not significantly changed. The above results demonstrate that the compounded plant extract of example 2 has the potential to improve the anxiety state of mice after sleep deprivation.

Test example 3 sensory evaluation was conducted on the compositions of examples 7 to 12

The composition is prepared by scoring 10 persons according to the scoring standards of table 1 for color, taste, flavor and condition of each composition; the score assigned weights are: the color and luster is 10 points, the taste is 40 points, the flavor is 30 points, the state is 20 points, and the full score is 100 points; the evaluation results are shown in Table 2.

TABLE 1 sensory evaluation Table

TABLE 2 sensory evaluation score results for each example

As can be seen from table 2, the composition of example 8 is uniform in color, clear, transparent, yellowish-brown in color, moderate in sweet-bitter ratio, fine and smooth in taste, has light traditional Chinese medicine fragrance, has no bad odor, ensures the sleep improvement and anxiety alleviation functions, has good flavor and taste, and is the optimal example.

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.