阅读说明：本技术 一种沙棘黄酮抗氧化电子烟烟液及其制备方法 (Seabuckthorn flavone antioxidant electronic cigarette liquid and preparation method thereof ) 是由 邹军 邹阳 刘梅森 于 2021-08-30 设计创作，主要内容包括：本申请涉及电子烟技术领域,具体公开了一种沙棘黄酮抗氧化电子烟烟液及其制备方法。电子烟烟液包括丙二醇48-70份、丙三醇12-30份、烟叶提取物3-12份、烟用香精1-6份、沙棘黄酮2-9份、香水莲花提取物1-8份；其制备方法为：首先将丙二醇、丙三醇、烟叶提取物、烟用香精混合均匀,制得基础烟液,将沙棘黄酮、香水莲花提取物加入到基础烟液中,混合均匀,制得电子烟烟液。本申请的一种沙棘黄酮抗氧化电子烟烟液,通过原料之间的协同作用,其具有增强电子烟烟液的口感品质及抗氧化作用的优点。(The application relates to the technical field of electronic cigarettes, and particularly discloses seabuckthorn flavone antioxidant electronic cigarette liquid and a preparation method thereof. The electronic cigarette liquid comprises 48-70 parts of propylene glycol, 12-30 parts of glycerol, 3-12 parts of tobacco leaf extract, 1-6 parts of tobacco essence, 2-9 parts of seabuckthorn flavone and 1-8 parts of nymphaea hybrid extract; the preparation method comprises the following steps: the method comprises the steps of firstly, uniformly mixing propylene glycol, glycerol, a tobacco leaf extract and tobacco essence to obtain a basic tobacco juice, adding the seabuckthorn flavone and the nymphaea hybrid extract into the basic tobacco juice, and uniformly mixing to obtain the electronic cigarette liquid. The sea buckthorn flavone antioxidant electronic cigarette liquid has the advantages that the taste quality and the antioxidant effect of the electronic cigarette liquid are enhanced through the synergistic effect of the raw materials.)

1. The sea-buckthorn flavone antioxidant electronic cigarette liquid is characterized in that: the composition is prepared from the following raw materials in parts by weight: 48-70 parts of propylene glycol, 12-30 parts of glycerol, 3-12 parts of tobacco leaf extract, 1-6 parts of tobacco essence, 2-9 parts of seabuckthorn flavone and 1-8 parts of nymphaea hybrid extract.

2. The seabuckthorn flavone antioxidant electronic cigarette liquid of claim 1, which is characterized in that: the composition is prepared from the following raw materials in parts by weight: 50-65 parts of propylene glycol, 15-25 parts of glycerol, 5-10 parts of tobacco leaf extract, 2-4 parts of tobacco essence, 3-7 parts of seabuckthorn flavone and 2-6 parts of nymphaea hybrid extract.

3. The seabuckthorn flavone antioxidant electronic cigarette liquid of claim 1, which is characterized in that: the tobacco leaf extract is a burley tobacco leaf extract.

4. The seabuckthorn flavone antioxidant electronic cigarette liquid of claim 3, which is characterized in that: the burley tobacco leaf extract is prepared by adopting the following method: drying the burley tobacco leaves at the temperature of 20-25 ℃ for 46-50h, then crushing, sieving by a sieve of 70-90 meshes, adding water, extracting by ultrasonic waves with the power of 150-200W at the temperature of 25-30 ℃ for 40-60min, filtering, collecting filtrate, and concentrating to 2-6% of the original volume at the negative pressure of (-65) - (-50) KPa and the temperature of 40-60 ℃ to obtain the burley tobacco leaf extract;

wherein burley tobacco leaf: the weight ratio of water is (1-3): (8-30).

5. The seabuckthorn flavone antioxidant electronic cigarette liquid of claim 1, which is characterized in that: the essence for the cigarettes is prepared from the following raw materials in parts by weight: 15-25 parts of propylene glycol, 3-8 parts of methyl p-aminobenzoate, 0.1-0.3 part of L-propylene glycol menthyl carbonate, 0.1-0.3 part of ethyl vanillin, 3-5 parts of ionone, 0.5-1 part of 2-acetylpyrazine, 0.8-2.2 parts of damascenone, 1-2 parts of clove oil, 7-12 parts of jujube tincture and 0.5-1.5 parts of liquorice fluid extract.

6. The seabuckthorn flavone antioxidant electronic cigarette liquid of claim 5, which is characterized in that: the tobacco essence is prepared by the following method: mixing propylene glycol, methyl p-aminobenzoate, L-menthyl carbonate, ethyl vanillin, ionone, 2-acetylpyrazine, damascenone, clove oil, jujube tincture and licorice fluid extract, and stirring to obtain the tobacco essence.

7. The seabuckthorn flavone antioxidant electronic cigarette liquid of claim 1, which is characterized in that: the seabuckthorn flavone is prepared by the following method:

s1: oven drying fructus Hippophae flavone fruit at 35-45 deg.C for 20-24 hr, pulverizing, and sieving with 50-70 mesh sieve to obtain fructus Hippophae flavone fruit powder;

s2: taking part of seabuckthorn flavone fruit powder, putting the seabuckthorn flavone fruit powder into an ethanol solution, extracting for 15-25min at the temperature of 30-70 ℃ by using ultrasonic waves with power of 350-;

s3: loading the crude extract to macroporous resin at flow rate of 1-2mL/min for cyclic adsorption for 10-14h, eluting with ethanol solution at flow rate of 1-2mL/min for 30-50min, collecting eluate, concentrating at negative pressure of (-90) - (-80) KPa and temperature of 35-60 deg.C to 25-35% of original volume, and drying at 25-55 deg.C for 46-50h to obtain fructus Hippophae flavone.

8. The seabuckthorn flavone antioxidant electronic cigarette liquid of claim 7, which is characterized in that: the volume of the ethanol solution added into each 1g of seabuckthorn flavone powder in the step S2 is 22-30mL, and the crude extraction solution in the step S3: the volume ratio of the ethanol solution is (60-80): (150-170), the mass concentration of the ethanol solution is 50-70%.

9. The seabuckthorn flavone antioxidant electronic cigarette liquid of claim 1, which is characterized in that: the nymphaea hybrid extract is prepared by adopting the following method: drying nymphaea hybrid at 60-65 deg.C for 20-28h, pulverizing, sieving with 50-70 mesh sieve to obtain nymphaea hybrid powder, adding ethanol solution into part of nymphaea hybrid powder, leaching at 55-65 deg.C for 1-3h to obtain crude extractive solution, concentrating the crude extractive solution at negative pressure of (-80) - (-70) KPa and 45-65 deg.C to 20-30% of the original volume, and drying the concentrate at 25-55 deg.C for 40-45h to obtain nymphaea hybrid extract;

wherein, the volume of the ethanol solution added into every 1g of the nymphaea hybrid powder is 5-7mL, and the mass concentration of the ethanol solution is 60-70%.

10. A method for preparing the seabuckthorn flavone antioxidant electronic cigarette liquid as claimed in any one of claims 1 to 9, which is characterized by comprising the following steps:

s1: mixing propylene glycol, glycerol, tobacco leaf extract and tobacco essence, and stirring to obtain basic tobacco liquid;

s2: adding fructus Hippophae flavone and herba Hylothelephii Verticillati extract into the basic tobacco juice, mixing, and stirring at 550r/min for 25-45min to obtain electronic tobacco juice.

Technical Field

The application relates to the technical field of electronic cigarettes, in particular to seabuckthorn flavone antioxidant electronic cigarette liquid and a preparation method thereof.

Background

With the development of the society and the pursuit of environmental protection and personal health, the electronic cigarette is more and more favored by consumers as a substitute of the traditional tobacco, the electronic cigarette does not generate harmful substances such as tar, and the tobacco liquid is atomized and discharged into the air, so that the harm of second-hand smoke is avoided.

The electronic cigarette liquid is a flavor source of the electronic cigarette, determines the flavor style of the electronic cigarette, and determines whether the product is accepted by consumers. At present, electronic cigarettes in the market are divided into a plurality of different tastes, and other electronic cigarettes have health care effects such as oxidation resistance, however, most electronic cigarettes with health care effects in the market may ignore the taste and quality of the electronic cigarettes, and cannot meet the requirements of smokers.

Disclosure of Invention

In order to enhance the taste quality and the antioxidation effect of the electronic cigarette liquid, the application provides the seabuckthorn flavone antioxidant electronic cigarette liquid and a preparation method thereof.

In a first aspect, the application provides a sea buckthorn flavone antioxidant electronic cigarette liquid, which adopts the following technical scheme: the sea-buckthorn flavone antioxidant electronic cigarette liquid is prepared from the following raw materials in parts by weight: 48-70 parts of propylene glycol, 12-30 parts of glycerol, 3-12 parts of tobacco leaf extract, 1-6 parts of tobacco essence, 2-9 parts of seabuckthorn flavone and 1-8 parts of nymphaea hybrid extract.

By adopting the technical scheme, the seabuckthorn flavone antioxidant electronic cigarette liquid has the advantages that the fragrance is fine and soft through the synergistic effect of the raw materials, the irritation and the miscellaneous smell are reduced, the aftertaste is more comfortable, the smoke richness is improved, and the requirements of smokers are met.

The propylene glycol, the glycerol and the tobacco leaf extract are used as basic components of the tobacco liquid, the tobacco leaf extract can increase the cigarette smell of the electronic cigarette, the mouth feel of the smoker after smoking is closer to that of the cigarette, the tobacco essence can be dissolved in the propylene glycol, the fragrance of the electronic cigarette is improved, and the requirements of the smoker on the electronic cigarette are met.

The sea-buckthorn is a medicine-food homologous plant, roots, stems, leaves, flowers and fruits of the sea-buckthorn, particularly the fruits of the sea-buckthorn contain rich nutrient substances and bioactive substances, and the modern pharmacological research shows that the sea-buckthorn has the effects of promoting blood circulation to reduce phlegm, relieving stuffiness and tonifying spleen and stomach, promoting the production of body fluid to quench thirst, clearing heat and checking diarrhea and preventing coronary heart disease; the seabuckthorn flavone is a flavonoid compound extracted from seabuckthorn fruits, is an active compound in seabuckthorn, can react with superoxide anions to prevent the initiation of free radical reaction, can prevent the generation of hydroxyl free radicals and lipid peroxidation radicals, can effectively remove the hydroxyl free radicals, has an antioxidant effect, can make the fragrance of the electronic cigarette more exquisite when being applied to the raw materials of the cigarette liquid of the electronic cigarette, increases the harmony and can meet the requirements of smokers.

The nymphaea hybrid has the summer-heat relieving and health-care effects, the nymphaea hybrid contains abundant polyphenols and flavonoids, has strong free radical scavenging capacity and biological activity, the polyphenols are natural antioxidants, and the nymphaea hybrid extract is mainly used for extracting the polyphenols and applied to raw materials of electronic cigarette liquid, so that the electronic cigarette liquid can be more delicate in taste, and the antioxidation effect of the electronic cigarette liquid can be enhanced.

Preferably, the feed additive is prepared from the following raw materials in parts by weight: 50-65 parts of propylene glycol, 15-25 parts of glycerol, 5-10 parts of tobacco leaf extract, 2-4 parts of tobacco essence, 3-7 parts of seabuckthorn flavone and 2-6 parts of nymphaea hybrid extract.

By adopting the technical scheme, the anti-oxidation effect of the electronic cigarette liquid can be improved by optimizing the weight ratio of the propylene glycol, the glycerol, the tobacco leaf extract, the tobacco essence, the seabuckthorn flavone and the nymphaea hybrid extract.

Preferably, the tobacco leaf extract is burley tobacco leaf extract.

By adopting the technical scheme, the tobacco leaf extract is used as a basic component of the electronic cigarette liquid, burley tobacco is high in moisture content, thin and light in leaf, loose and not rough in tissue structure, strong in elasticity, high in filling value, good in spice absorption performance and outstanding in style, and the burley tobacco leaf extract is added into the raw materials of the electronic cigarette liquid, so that the electronic cigarette liquid can be endowed with fragrance, and the requirements of smokers can be met conveniently.

Preferably, the burley tobacco leaf extract is prepared by the following method: drying the burley tobacco leaves at the temperature of 20-25 ℃ for 46-50h, then crushing, sieving by a sieve of 70-90 meshes, adding water, extracting by ultrasonic waves with the power of 150-200W at the temperature of 25-30 ℃ for 40-60min, filtering, collecting filtrate, and concentrating to 2-6% of the original volume at the negative pressure of (-65) - (-50) KPa and the temperature of 40-60 ℃ to obtain the burley tobacco leaf extract;

wherein burley tobacco leaf: the weight ratio of water is (1-3): (8-30).

By adopting the technical scheme, the burley tobacco leaf extract is prepared by utilizing the raw materials, has the advantage of simple and convenient preparation, and can better play a role of the burley tobacco leaf extract.

Preferably, the essence for tobacco is prepared from the following raw materials in parts by weight: 15-25 parts of propylene glycol, 3-8 parts of methyl p-aminobenzoate, 0.1-0.3 part of L-propylene glycol menthyl carbonate, 0.1-0.3 part of ethyl vanillin, 3-5 parts of ionone, 0.5-1 part of 2-acetylpyrazine, 0.8-2.2 parts of damascenone, 1-2 parts of clove oil, 7-12 parts of jujube tincture and 0.5-1.5 parts of liquorice fluid extract.

By adopting the technical scheme, the methyl p-aminobenzoate can be dissolved in the propylene glycol, and the L-propylene glycol menthyl carbonate is applied to the raw materials of the electronic cigarette liquid, so that the fragrance of mint in the electronic cigarette liquid can be increased, and a smoker can feel cool after smoking; the ethyl vanillin has strong vanilla bean fragrance, has the advantage of lasting fragrance, can enhance fragrance lasting time, and also has the function of fixing fragrance; the ionone has costustoot and strong ionone fragrance, is in the fragrance of iris root after being diluted, can be dissolved in propylene glycol, can endow the electronic cigarette liquid with fragrance, and is convenient for meeting the requirements of smokers; the 2-acetylpyrazine has baking flavor, and can increase the aroma of the electronic cigarette liquid when being added into the raw materials of the electronic cigarette liquid; the damascenone has the fragrance of apples, strawberries, roses and the like, not only can the fragrance of the tobacco juice be enhanced, but also the fragrance of the electronic cigarette tobacco juice can be more full, and the taste of the electronic cigarette tobacco juice can be improved; the clove oil has the special aromatic smell of clove, the jujube tincture has the sweet taste and faint scent of red jujube, and when the jujube tincture is applied to the cigarette liquid of the electronic cigarette, the pungent and sweet fragrance can be increased, and the miscellaneous gas of the cigarette liquid of the electronic cigarette can be covered; the liquorice fluid extract has the characteristic of sweet taste, can play roles in increasing aroma, seasoning, keeping moisture and fixing aroma, and also can play roles in enhancing sweet feeling and reducing smoke stimulation; through the synergistic effect among the raw materials, the aroma of the cigarette liquid of the electronic cigarette can be increased, the taste of the electronic cigarette is closer to that of a cigarette, and the requirements of smokers are met conveniently.

Preferably, the tobacco essence is prepared by the following method: mixing propylene glycol, methyl p-aminobenzoate, L-menthyl carbonate, ethyl vanillin, ionone, 2-acetylpyrazine, damascenone, clove oil, jujube tincture and licorice fluid extract, and stirring to obtain the tobacco essence.

By adopting the technical scheme, the tobacco essence is prepared by utilizing the raw materials, so that the tobacco essence has the advantage of simple and convenient preparation, is convenient for mixing the tobacco essence more uniformly, and is favorable for bringing the tobacco essence into play more.

Preferably, the seabuckthorn flavone is prepared by the following method:

s1: oven drying fructus Hippophae flavone fruit at 35-45 deg.C for 20-24 hr, pulverizing, and sieving with 50-70 mesh sieve to obtain fructus Hippophae flavone fruit powder;

s2: taking part of seabuckthorn flavone fruit powder, putting the seabuckthorn flavone fruit powder into an ethanol solution, extracting for 15-25min at the temperature of 30-70 ℃ by using ultrasonic waves with power of 350-;

s3: loading the crude extract to macroporous resin at flow rate of 1-2mL/min for cyclic adsorption for 10-14h, eluting with ethanol solution at flow rate of 1-2mL/min for 30-50min, collecting eluate, concentrating at negative pressure of (-90) - (-80) KPa and temperature of 35-60 deg.C to 25-35% of original volume, and drying at 25-55 deg.C for 46-50h to obtain fructus Hippophae flavone.

By adopting the technical scheme, the raw materials are utilized to prepare the seabuckthorn flavone, so that the preparation method has the advantage of simple and convenient preparation, and the seabuckthorn flavone plays a better role.

Preferably, the volume of the ethanol solution added into each 1g of seabuckthorn flavone powder in the step S2 is 22-30mL, and the crude extraction solution in the step S3: the volume ratio of the ethanol solution is (60-80): (150-170), the mass concentration of the ethanol solution is 50-70%.

By adopting the technical scheme, the weight ratio of the seabuckthorn flavone fruit powder, the ethanol solution and the crude extraction liquid is optimized, so that the seabuckthorn flavone can play a better role, and the antioxidation of the electronic cigarette liquid is favorably enhanced.

Preferably, the nymphaea hybrid extract is prepared by the following method: drying nymphaea hybrid at 60-65 deg.C for 20-28h, pulverizing, sieving with 50-70 mesh sieve to obtain nymphaea hybrid powder, adding ethanol solution into part of nymphaea hybrid powder, leaching at 55-65 deg.C for 1-3h to obtain crude extractive solution, concentrating the crude extractive solution at negative pressure of (-80) - (-70) KPa and 45-65 deg.C to 20-30% of the original volume, and drying the concentrate at 25-55 deg.C for 40-45h to obtain nymphaea hybrid extract;

wherein, the volume of the ethanol solution added into every 1g of the nymphaea hybrid powder is 5-7mL, and the mass concentration of the ethanol solution is 60-70%.

By adopting the technical scheme, the raw materials are utilized to prepare the nymphaea hybrid extract, the nymphaea hybrid extract has the advantage of simple and convenient preparation, can better play a role, and is added into the raw materials of the electronic cigarette liquid, so that the antioxidation of the electronic cigarette liquid is conveniently enhanced.

In a second aspect, the application provides a preparation method of seabuckthorn flavone antioxidant electronic cigarette liquid, which adopts the following technical scheme:

a preparation method of seabuckthorn flavone antioxidant electronic cigarette liquid comprises the following steps:

s1: mixing propylene glycol, glycerol, tobacco leaf extract and tobacco essence, and stirring to obtain basic tobacco liquid;

s2: adding fructus Hippophae flavone and herba Hylothelephii Verticillati extract into the basic tobacco juice, mixing, and stirring at 550r/min for 25-45min to obtain electronic tobacco juice.

By adopting the technical scheme, the propylene glycol, the glycerol, the tobacco leaf extract and the tobacco essence are uniformly mixed to prepare the basic tobacco juice, the seabuckthorn flavone and the nymphaea hybrid flower extract are prepared and mixed with the basic tobacco juice, so that the mixing is more uniform, the seabuckthorn flavone and the nymphaea hybrid flower extract can play a larger role, the fragrance of the electronic cigarette tobacco juice can be increased, and the antioxidation of the electronic cigarette tobacco juice is enhanced.

In summary, the present application has the following beneficial effects:

1. the application adopts the seabuckthorn flavone and the balm flower extract, the seabuckthorn flavone is a flavonoid compound extracted from seabuckthorn fruits, can react with superoxide anions to prevent the initiation of free radical reaction, prevent the generation of hydroxyl free radicals and lipid peroxidation radicals, and can effectively remove the hydroxyl free radicals, thereby having the effect of oxidation resistance; the nymphaea hybrid cigarette contains abundant polyphenol and flavonoid substances, has strong free radical scavenging capacity and biological activity, and can make the fragrance of the electronic cigarette softer, make the aftertaste more comfortable and strengthen the antioxidation of the cigarette liquid of the electronic cigarette by mainly extracting the polyphenol.

2. Propylene glycol, methyl p-aminobenzoate, L-menthyl carbonate, ethyl vanillin, ionone, 2-acetylpyrazine, damascenone, clove oil, jujube tincture and liquorice fluid extract are preferably adopted as raw materials of the tobacco essence, and through the synergistic effect of the raw materials, the aroma of the electronic cigarette liquid can be increased, the taste of the electronic cigarette liquid is improved, and the requirements of smokers are met.

Detailed Description

The present application will be described in further detail with reference to examples.

The raw material burley tobacco is selected from the limited liability company of the burley tobacco in Yunnan; the methyl p-aminobenzoate is selected from Nicotiana tabaci chemical technology Co; l-propylene glycol menthyl carbonate is selected from Shandong Moore chemical Co., Ltd; the ethyl vanillin is selected from Shenzhen Lefu biotechnology and science and technology Limited; the ionone is selected from Shandong Teng Wang chemical Co Ltd; the 2-acetylpyrazine is selected from Hubei Hengcheng science and technology limited; damascenone is selected from the group consisting of Hubei Handa Biotech, Inc.; the clove oil is selected from Nantong Runfeng petrochemical company; the fructus Jujubae tincture is selected from Guangzhou Jiarui science and technology development limited; the Glycyrrhrizae radix fluid extract is selected from Sienna Bei Ji Nuo biological technology Co., Ltd; the macroporous resin is selected from Gallery Shengquan chemical industry Co; the nymphaea hybrid is selected from Fujian Zhangzhou nymphaea hybrid resource garden.

Preparation example

Preparation example 1

A burley tobacco leaf extract, which is prepared by the following method:

drying 1Kg of burley tobacco leaves at 20 deg.C for 46h, pulverizing, sieving with 70 mesh sieve, adding 8Kg of water, extracting at 25 deg.C with 150W ultrasonic wave for 40min, filtering, collecting filtrate, concentrating under-65 KPa negative pressure at 40 deg.C to 2% of the original volume to obtain the extract.

Preparation example 2

A burley tobacco leaf extract, which is prepared by the following method:

drying 2Kg of burley tobacco leaves at 23 ℃ for 48h, then crushing, sieving with a 80-mesh sieve, adding 18Kg of water, extracting at 27 ℃ with ultrasonic waves with the power of 175W for 50min, filtering, collecting the filtrate, concentrating to 4% of the original volume at the temperature of 50 ℃ under the negative pressure of-57 KPa, and obtaining the burley tobacco leaf extract.

Preparation example 3

A burley tobacco leaf extract, which is prepared by the following method:

drying 3Kg of burley tobacco leaves at 25 ℃ for 50h, then crushing, sieving with a 90-mesh sieve, adding 30Kg of water, extracting at 30 ℃ with 200W ultrasonic wave for 60min, filtering, collecting the filtrate, concentrating to 6% of the original volume at-50 KPa negative pressure and 60 ℃ to obtain the burley tobacco leaf extract.

Preparation example 4

The tobacco essence is prepared by the following method:

15Kg of propylene glycol, 3Kg of methyl p-aminobenzoate, 0.1Kg of 0.1 KgL-propylene glycol menthyl carbonate, 0.1Kg of ethyl vanillin, 3Kg of ionone, 0.5Kg of 2-acetylpyrazine, 0.8Kg of damascenone, 1Kg of clove oil, 7Kg of jujube tincture and 0.5Kg of liquorice fluid extract are mixed and stirred uniformly to prepare the tobacco essence.

Preparation example 5

The tobacco essence is prepared by the following method:

20Kg of propylene glycol, 5Kg of methyl p-aminobenzoate, 0.2 KgL-propylene glycol menthyl carbonate, 0.2Kg of ethyl vanillin, 4Kg of ionone, 0.7Kg of 2-acetylpyrazine, 1.5Kg of damascenone, 1.5Kg of clove oil, 9Kg of jujube tincture and 1Kg of liquorice fluid extract are mixed and stirred uniformly to prepare the tobacco essence.

Preparation example 6

The tobacco essence is prepared by the following method:

mixing 25Kg of propylene glycol, 8Kg of methyl p-aminobenzoate, 0.3Kg of 0.3 KgL-propylene glycol menthyl carbonate, 0.3Kg of ethyl vanillin, 5Kg of ionone, 1Kg of 2-acetylpyrazine, 2.2Kg of damascenone, 2Kg of clove oil, 12Kg of jujube tincture and 1.5Kg of liquorice fluid extract, and stirring uniformly to obtain the tobacco essence.

Preparation example 7

A seabuckthorn flavone is prepared by the following steps:

s1: drying fructus Hippophae flavone fruit at 35 deg.C for 20 hr, pulverizing, and sieving with 50 mesh sieve to obtain fructus Hippophae flavone powder;

s2: putting 15g of seabuckthorn flavone powder into 450mL of ethanol solution, extracting for 15min at 30 ℃ by using ultrasonic waves with power of 350W, centrifuging for 8min at the rotating speed of 3500r/min, taking supernate, and concentrating to the relative density of 1.05 at 65 ℃ to obtain crude extract;

s3: loading 60mL of crude extract to macroporous resin at the flow rate of 1mL/min for cyclic adsorption for 10h, eluting with 150mL of ethanol solution at the flow rate of 1mL/min for 30min, collecting eluate, concentrating at-90 KPa negative pressure and 35 deg.C to 25% of original volume, and drying the concentrate at 25 deg.C for 46h to obtain fructus Hippophae flavone;

wherein the mass concentration of the ethanol solution is 50%.

Preparation example 8

A seabuckthorn flavone is prepared by the following steps:

s1: drying fructus Hippophae flavone fruit at 40 deg.C for 22 hr, pulverizing, and sieving with 60 mesh sieve to obtain fructus Hippophae flavone powder;

s2: putting 20g of seabuckthorn flavone powder into 500mL of ethanol solution, extracting for 20min at 50 ℃ by using ultrasonic waves with 400W power, centrifuging for 10min at the rotating speed of 4000r/min, taking supernate, and concentrating until the relative density is 1.15 at 65 ℃ to obtain crude extract;

s3: loading 70mL of crude extract to macroporous resin at a flow rate of 1.5mL/min for cyclic adsorption for 12h, eluting with 160mL of ethanol solution at a flow rate of 1.5mL/min for 40min, collecting eluate, concentrating at-85 KPa negative pressure and 47 deg.C to 30% of original volume, and drying the concentrate at 40 deg.C for 48h to obtain fructus Hippophae flavone;

wherein the mass concentration of the ethanol solution is 60 percent.

Preparation example 9

A seabuckthorn flavone is prepared by the following steps:

s1: drying fructus Hippophae flavone fruit at 45 deg.C for 24 hr, pulverizing, and sieving with 70 mesh sieve to obtain fructus Hippophae flavone powder;

s2: putting 25g of seabuckthorn flavone powder into 550mL of ethanol solution, extracting for 25min at 70 ℃ by using ultrasonic waves with power of 450W, centrifuging for 12min at the rotating speed of 4500r/min, taking supernate, and concentrating to the relative density of 1.25 at 65 ℃ to obtain crude extract;

s3: loading 80mL of crude extract to macroporous resin at a flow rate of 2mL/min for cyclic adsorption for 14h, eluting with 170mL of ethanol solution at a flow rate of 2mL/min for 50min, collecting eluate, concentrating at-80 KPa negative pressure and 60 deg.C to 35% of original volume, and drying the concentrate at 55 deg.C for 50h to obtain fructus Hippophae flavone;

wherein the mass concentration of the ethanol solution is 70 percent.

Preparation example 10

A nymphaea hybrid extract is prepared by adopting the following method:

cleaning nymphaea hybrid, drying at 60 ℃ for 20h, then crushing, sieving with a 50-mesh sieve to obtain nymphaea hybrid powder, taking 10g of nymphaea hybrid powder, adding 50mL of ethanol solution, leaching at 55 ℃ for 1h to obtain crude extraction liquid, concentrating the crude extraction liquid to 20% of the original volume at negative pressure of-80 KPa and 45 ℃, and drying the concentrate at 25 ℃ for 40h to obtain the nymphaea hybrid extract;

wherein the mass concentration of the ethanol solution is 60 percent.

Preparation example 11

A nymphaea hybrid extract is prepared by adopting the following method:

cleaning nymphaea hybrid, drying the nymphaea hybrid at 63 ℃ for 24h, then crushing the nymphaea hybrid, sieving the nymphaea hybrid with a 60-mesh sieve to obtain nymphaea hybrid powder, adding 10g of the nymphaea hybrid powder into 60mL of ethanol solution, leaching the nymphaea hybrid powder at 60 ℃ for 2h to obtain crude extract, concentrating the crude extract to 25% of the original volume at the negative pressure of-75 KPa and the temperature of 55 ℃, and drying the concentrate at the temperature of 40 ℃ for 43h to obtain the nymphaea hybrid extract;

wherein the mass concentration of the ethanol solution is 65 percent.

Preparation example 12

A nymphaea hybrid extract is prepared by adopting the following method:

cleaning nymphaea hybrid, drying for 28h at 65 ℃, then crushing, sieving by a 70-mesh sieve to obtain nymphaea hybrid powder, taking 10g of nymphaea hybrid powder, adding 70mL of ethanol solution, leaching for 3h at 65 ℃ to obtain crude extraction liquid, concentrating the crude extraction liquid to 30% of the original volume at negative pressure of-70 KPa and the temperature of 65 ℃, and drying the concentrate for 45h at 55 ℃ to obtain the nymphaea hybrid extract;

wherein the mass concentration of the ethanol solution is 70 percent.

Examples

Table 1 example the weight of each raw material of tobacco liquid (unit: Kg)

Example 1

The raw material proportion of the seabuckthorn flavone antioxidant electronic cigarette liquid is shown in table 1.

Wherein the burley tobacco extract is prepared by the preparation example 1, the tobacco essence is prepared by the preparation example 4, the seabuckthorn flavone is prepared by the preparation example 7, and the nymphaea hybrid extract is prepared by the preparation example 10.

A preparation method of seabuckthorn flavone antioxidant electronic cigarette liquid comprises the following steps:

s1: mixing propylene glycol, glycerol, tobacco leaf extract and tobacco essence, and stirring to obtain basic tobacco liquid;

s2: adding fructus Hippophae flavone and herba seu radix Heteropapyridis Incarnatae extract into the basic tobacco juice, mixing, and stirring at 250r/min for 25min to obtain electronic tobacco juice.

Examples 2 to 6

The sea buckthorn flavone antioxidant electronic cigarette liquid is different from the electronic cigarette liquid in the raw material ratio shown in the table 1.

Example 7

An anti-oxidation seabuckthorn flavone electronic cigarette liquid is different from the preparation method of the burley tobacco extract in the embodiment 5, and is prepared by adopting the preparation example 2.

Example 8

An anti-oxidation seabuckthorn flavone electronic cigarette liquid is different from the preparation method of the burley tobacco extract in the embodiment 5, and is prepared by adopting the preparation embodiment 3.

Example 9

A sea buckthorn flavone antioxidant electronic cigarette liquid is different from the electronic cigarette liquid in example 7 in raw material difference and is prepared by adopting preparation example 5.

Example 10

A sea buckthorn flavone antioxidant electronic cigarette liquid is different from the electronic cigarette liquid in example 7 in raw material difference and is prepared by adopting preparation example 6.

Example 11

An antioxidant seabuckthorn flavone electronic cigarette liquid is different from the seabuckthorn flavone preparation method in embodiment 10 and is prepared by the preparation method in preparation 8.

Example 12

An antioxidant seabuckthorn flavone electronic cigarette liquid is different from the seabuckthorn flavone preparation method in embodiment 10 and is prepared by the preparation method in embodiment 9.

Example 13

An anti-oxidation seabuckthorn flavone electronic cigarette liquid is different from that in example 12 in that a preparation method of a nymphaea hybrid extract is adopted, and the seabuckthorn flavone anti-oxidation electronic cigarette liquid is prepared in preparation example 11.

Example 14

An anti-oxidation seabuckthorn flavone electronic cigarette liquid is different from that in the embodiment 12 in the preparation method of a nymphaea hybrid extract, and is prepared by the preparation method in the embodiment 12.

Example 15

An anti-oxidation seabuckthorn flavone electronic cigarette liquid is different from the electronic cigarette liquid in embodiment 14 in that the same amount of seabuckthorn flavone is used for replacing a nymphaea hybrid extract in the raw materials of the electronic cigarette liquid.

Example 16

An anti-oxidation seabuckthorn flavone electronic cigarette liquid is different from the electronic cigarette liquid in embodiment 14 in that seabuckthorn flavone is replaced by an equivalent amount of nymphaea hybrid extract in raw materials of the electronic cigarette liquid.

Example 17

A seabuckthorn flavone antioxidant electronic cigarette liquid, which is different from the electronic cigarette liquid in the preparation method in example 14.

A preparation method of seabuckthorn flavone antioxidant electronic cigarette liquid comprises the following steps:

s1: mixing propylene glycol, glycerol, tobacco leaf extract and tobacco essence, and stirring to obtain basic tobacco liquid;

s2: adding fructus Hippophae flavone and herba seu radix Heteropapyridis Incarnatae extract into the basic tobacco juice, mixing, and stirring at 400r/min for 35min to obtain electronic tobacco juice.

Example 18

A seabuckthorn flavone antioxidant electronic cigarette liquid, which is different from the electronic cigarette liquid in the preparation method in example 14.

A preparation method of seabuckthorn flavone antioxidant electronic cigarette liquid comprises the following steps:

s1: mixing propylene glycol, glycerol, tobacco leaf extract and tobacco essence, and stirring to obtain basic tobacco liquid;

s2: adding fructus Hippophae flavone and herba seu radix Heteropapyridis Incarnatae extract into the basic tobacco juice, mixing, and stirring at 550r/min for 45min to obtain electronic tobacco juice.

Comparative example

Comparative example 1

A seabuckthorn flavone oxidation resistant electronic cigarette liquid, which is different from the electronic cigarette liquid in embodiment 17 in that the seabuckthorn flavone is replaced by propylene glycol with the same amount in the raw materials of the electronic cigarette liquid.

Comparative example 2

A sea buckthorn flavone antioxidant electronic cigarette liquid, which is different from the electronic cigarette liquid in embodiment 17 in that the raw material of the electronic cigarette liquid is replaced by an equivalent amount of propylene glycol instead of a nymphaea hybrid extract.

Comparative example 3

A seabuckthorn flavone antioxidant electronic cigarette liquid, which is different from the electronic cigarette liquid in embodiment 17 in that the seabuckthorn flavone and the nymphaea hybrid extract are replaced by the same amount of propylene glycol in the raw materials of the electronic cigarette liquid.

Performance test

Samples were prepared from portions of the electronic cigarette liquids obtained in examples 1 to 18 and comparative examples 1 to 3, and the following performance tests were performed, and the test results are shown in table 4.

Detection method

Selecting 36 smokers, sucking the cigarette liquid of the electronic cigarette by using a three-section smoking evaluation method according to the appearance index in the table 2 and the score in the table 3 and recording the score in the table 4, wherein each part of the look and feel index and the grade classification table of the seabuckthorn flavone antioxidant cigarette liquid is obtained.

TABLE 2 look and feel index and grade classification table

TABLE 3 three-stage score distribution chart for evaluation

The score calculation mode is as follows:

initial stage-smelling fragrance/10 × 5+ inlet fragrance/20 × 10+ inlet fragrance/40 × 15-30 ═ 30

Intermediate stage of flavor/40 × 25+ mouthfeel/30 × 25 ═ 50

Tail segment of miscellaneous taste/10 × 5+ residual taste/10 × 15 ═ 20

The total score is 100 in initial stage + intermediate stage + end stage

TABLE 4 sensory grade recording sheet

As can be seen from table 4, the seabuckthorn flavone antioxidant electronic cigarette liquid disclosed by the application has the advantages that the fragrance is finer and softer through the synergistic effect of the raw materials, the irritation and the miscellaneous odor are reduced, the aftertaste is more comfortable, the smoke richness is improved, and the requirements of smokers are met.

By comparing the example 17 with the comparative examples 1 to 3, it can be seen that the seabuckthorn flavone and the nymphaea hybrid extract are added into the raw materials of the electronic cigarette liquid, and the quality of the electronic cigarette liquid is obviously improved and the antioxidation of the electronic cigarette liquid is also enhanced through the synergistic effect between the seabuckthorn flavone and the nymphaea hybrid extract.

The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.