阅读说明：本技术 一种牛大力中总皂苷的超声-微波协同提取方法 (Ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root ) 是由 王茂媛 羊青 王清隆 王祝年 晏小霞 汤欢 李英英 王建荣 于 2021-09-18 设计创作，主要内容包括：本发明提供一种牛大力中总皂苷的超声-微波协同提取方法,包括以下步骤：S1材料处理、S2冷浸、S3酶水解、S4提取浸膏、S5超声-微波协同提取,本发明的提取方法提取总皂苷含量较高,皂苷收率较高,而杂质含量少,各个步骤协同作用,解决总皂苷含量较低、杂质较多、提取过程起泡的问题。(The invention provides an ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root, which comprises the following steps: s1 material processing, S2 cold soaking, S3 enzyme hydrolysis, S4 extract extraction and S5 ultrasonic-microwave synergistic extraction.)

1. An ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root is characterized in that: the method comprises the following steps:

s1, material treatment: cleaning and airing the beautiful millettia root medicinal material, conveying the medicinal material into a dryer, and continuously and dynamically drying the medicinal material by hot air at 40-60 ℃ until the moisture of the beautiful millettia root is lower than 10% to obtain dried beautiful millettia root;

s2, cold soaking: cutting the dried beautiful millettia root into sections, putting the sections into a dichloromethane solvent with the volume fraction of 70-90%, cold-soaking for 1-3 days for the first time, stirring for 2-4 hours, cold-soaking for 2-4 days for the second time, stirring for 1-2 hours, and filtering to obtain the beautiful millettia root cold-soaking section;

s3, enzyme hydrolysis: mixing yeast protease and distilled water to prepare a yeast protease solution, soaking the beautiful millettia root cold soaking section in the yeast protease solution, controlling the temperature to be 30-50 ℃, controlling the relative humidity to be 60-100% and the pressure to be 1.2-2.0 MPa, and carrying out pressurized hydrolysis for 2-4 h;

s4, extracting an extract: adding an ether solvent into the millettia speciosa champ subjected to enzymatic hydrolysis, stirring and reflux-extracting for 30-50 min at 38-45 ℃, filtering, collecting residues for later use, and concentrating the filtrate under reduced pressure to obtain millettia speciosa champ extract;

s5, ultrasonic-microwave synergistic extraction: mixing the S4 radix millettiae speciosae extract and water-saturated n-butanol according to the mass volume g/mL ratio of 1: 3-5, putting the mixture into an ultrasonic and microwave synergistic extraction tank for extraction, collecting an upper phase, concentrating and precipitating to obtain the radix millettiae speciosae total saponins.

2. The ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root as claimed in claim 1, characterized in that: the mass-volume ratio g/mL of the dried radix millettiae speciosae and the dichloromethane solvent of S2 is 0.3-0.8: 1 to 5.

3. The ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root as claimed in claim 1, characterized in that: the S3 yeast protease solution is prepared by mixing yeast protease and distilled water according to the volume ratio of 1: 5-12 by mixing.

4. The ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root as claimed in claim 1, characterized in that: the mass-volume ratio g/mL of the bovine fertility hydrolyzed by the S4 enzyme to the ether solvent is 1: 3-8.

5. The ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root as claimed in claim 1, characterized in that: in the S5 ultrasonic-microwave synergistic extraction, the microwave frequency is 1500-2000 MHz, and the power is 2-8 KW.

6. The ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root as claimed in claim 1, characterized in that: and in the S5 ultrasonic-microwave synergistic extraction, the ultrasonic frequency is 30-60 KHz, and the power is 0.8-2 KW.

7. The ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root as claimed in claim 1, characterized in that: the temperature of the S5 ultrasonic-microwave synergistic extraction is 40-50 ℃, and the extraction time is 20-60 min.

Technical Field

The invention relates to the field of plant extraction, and particularly relates to an ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root.

Background

Beautiful millettia root, radix Stephaniae Cepharanthae, rhizoma Nelumbinis, radix Stephaniae Tetrandrae, and DALI. The active ingredients of the Millettia speciosa champ are mainly flavonoids, saccharides, saponins and alkaloid compounds, so that the Millettia speciosa champ is a plant resource with a certain health care effect, and is worthy of deep research and wide popularization. The existing extraction method of the beautiful millettia root total saponins almost analyzes saponin components in alcohol soluble components, the content of the extracted total saponins is 50-80%, even if the high-content total saponins are obtained, column chromatography is carried out for many times, the method directly separates and purifies the extracting solution containing a large amount of impurities through a macroporous adsorption resin column, so that the problems of great loss of effective components while impurity removal is caused, high cost and incomplete impurity removal exist; patent CN102793741A discloses a beautiful millettia root total component extract and a preparation method and application thereof, and provides an alcohol extraction method of beautiful millettia root components, but the effect on the extraction process of saponin is poor; patent CN110025645B discloses a method for extracting total saponins of panax quinquefolium, which has low purity of extracted saponins through multiple column chromatography.

Disclosure of Invention

In view of the above, the invention provides an ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root, which solves the above problems.

The technical scheme of the invention is realized as follows: an ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root comprises the following steps: the method comprises the following steps:

s1, material treatment: cleaning and airing the beautiful millettia root medicinal material, conveying the medicinal material into a dryer, and continuously and dynamically drying the medicinal material by hot air at 40-60 ℃ until the moisture of the beautiful millettia root is lower than 10% to obtain dried beautiful millettia root;

s2, cold soaking: cutting the dried beautiful millettia root into sections, putting the sections into a dichloromethane solvent with the volume fraction of 70-90%, cold-soaking for 1-3 days for the first time, stirring for 2-4 hours, cold-soaking for 2-4 days for the second time, stirring for 1-2 hours, and filtering to obtain the beautiful millettia root cold-soaking section;

s3, enzyme hydrolysis: mixing yeast protease and distilled water to prepare a yeast protease solution, soaking the beautiful millettia root cold soaking section in the yeast protease solution, controlling the temperature to be 30-50 ℃, controlling the relative humidity to be 60-100% and the pressure to be 1.2-2.0 MPa, and carrying out pressurized hydrolysis for 2-4 h;

s4, extracting an extract: adding an ether solvent into the millettia speciosa champ subjected to enzymatic hydrolysis, stirring and reflux-extracting for 30-50 min at 38-45 ℃, filtering, collecting residues for later use, and concentrating the filtrate under reduced pressure to obtain millettia speciosa champ extract; the millettia speciosa champ is added with an ether solvent and hydrolyzed by enzyme, so that fat-soluble impurities can be removed;

s5, ultrasonic-microwave synergistic extraction: mixing the S4 radix millettiae speciosae extract with water-saturated n-butanol according to the mass volume g/mL ratio of 1: 3-5, putting the mixture into an ultrasonic and microwave synergistic extraction tank for extraction, collecting an upper phase, concentrating and precipitating to obtain total saponins of radix millettiae speciosae;

further, the mass-volume ratio g/mL of the dried radix millettiae speciosae S2 to the dichloromethane solvent is 0.3-0.8: 1 to 5.

Further, the S3 yeast protease solution is prepared by mixing yeast protease and distilled water in a volume ratio of 1: 5-12 by mixing.

Further, the mass-volume ratio g/mL of the bovine fertility subjected to S4 enzyme hydrolysis to the ether solvent is 1: 3-8

Further, in the S5 ultrasonic-microwave synergistic extraction, the microwave frequency is 1500-2000 MHz, and the power is 2-8 KW.

Further, in the S5 ultrasonic-microwave synergistic extraction, the ultrasonic frequency is 30-60 KHz, and the power is 0.8-2 KW.

Further, the temperature of the ultrasonic-microwave synergistic extraction of S5 is 40-50 ℃, and the extraction time is 20-60 min.

Compared with the prior art, the invention has the beneficial effects that:

the ultrasonic-microwave synergistic extraction method of the total saponin in the millettia speciosa champ is high in total saponin content, high in saponin yield and low in impurity content, the millettia speciosa champ is dried and then is subjected to cold soaking, epidermal cells of the millettia speciosa champ are broken, the adsorption force between chemical components and histiocytes in the millettia speciosa champ is overcome, effective components in the millettia speciosa champ are extracted while floccules such as pectin and the like are removed, an enzyme protease solution is added for enzymatic hydrolysis, sapogenin is obtained by hydrolyzing the saponin, subsequent extraction is facilitated, the reflux extraction of the saponin by using an ether solvent is facilitated to remove fat-soluble impurities, finally, the ultrasonic-microwave synergistic extraction is used, the frequency and the power are controlled, the synergistic extraction is capable of inhibiting the foaming problem of the saponin in the extraction process, and the purity of the saponin is improved.

Detailed Description

In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.

The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.

The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.

Example 1

An ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root comprises the following steps:

s1, material treatment: cleaning and airing the beautiful millettia root medicinal material, conveying the medicinal material into a dryer, and continuously and dynamically drying the medicinal material by hot air at 40 ℃ until the moisture of the beautiful millettia root is lower than 10 percent to obtain dried beautiful millettia root;

s2, cold soaking: cutting the dried beautiful millettia root into segments, putting the segments into a dichloromethane solvent with the volume fraction of 70 percent for cold soaking, wherein the mass volume ratio g/mL of the dried beautiful millettia root to the dichloromethane solvent is 0.3: 1, stirring for 2 hours after primary cold soaking for 1 day, stirring for 1 hour after secondary cold soaking for 2 days, and filtering to obtain a beautiful millettia root cold soaking section;

s3, enzyme hydrolysis: according to the volume ratio of 1: 5-12, mixing the yeast protease with distilled water to prepare a yeast protease solution, soaking the beautiful millettia root cold soaking section in the yeast protease solution, controlling the temperature to be 30 ℃, controlling the relative humidity to be 60% and the pressure to be 1.2MPa, and carrying out pressurized hydrolysis for 2 hours;

s4, extracting an extract: adding an ether solvent into the millettia speciosa champ subjected to enzymatic hydrolysis according to the mass volume ratio of g/mL of 1:3, stirring and reflux-extracting for 30min at 38 ℃, filtering, collecting residues for later use, and concentrating the filtrate under reduced pressure to obtain millettia speciosa champ extract;

s5, ultrasonic-microwave synergistic extraction: mixing the S4 radix Millettiae Speciosae extract with water-saturated n-butanol at a mass volume g/mL ratio of 1:3, extracting in an ultrasonic and microwave synergistic extraction tank at 1500MHz and 2KW respectively at 30KHz and 0.8KW respectively at 40 deg.C for 20min, collecting the upper phase, concentrating, and precipitating to obtain radix Millettiae Speciosae total saponin.

Example 2

An ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root comprises the following steps:

s1, material treatment: cleaning and airing the beautiful millettia root medicinal material, conveying the medicinal material into a dryer, and continuously and dynamically drying the medicinal material by hot air at the temperature of 60 ℃ until the moisture of the beautiful millettia root is lower than 10 percent to obtain dried beautiful millettia root;

s2, cold soaking: cutting the dried beautiful millettia root into segments, putting the segments into a dichloromethane solvent with the volume fraction of 90%, and cold soaking, wherein the mass-volume ratio g/mL of the dried beautiful millettia root to the dichloromethane solvent is 0.8: 5, stirring for 4 hours after primary cold soaking for 3 days, stirring for 2 hours after secondary cold soaking for 4 days, and filtering to obtain a beautiful millettia root cold soaking section;

s3, enzyme hydrolysis: according to the volume ratio of 1: 12, mixing the yeast protease with distilled water to prepare a yeast protease solution, soaking the cold soaking section of the beautiful millettia root in the yeast protease solution, controlling the temperature to be 30-50 ℃, controlling the relative humidity to be 100% and the pressure to be 2.0MPa, and carrying out pressurized hydrolysis for 4 hours;

s4, extracting an extract: adding an ether solvent into the millettia speciosa champ subjected to enzyme hydrolysis according to the mass volume ratio of g/mL of 1:8, stirring and reflux-extracting for 50min at 45 ℃, filtering, collecting residues for later use, and concentrating the filtrate under reduced pressure to obtain millettia speciosa champ extract;

s5, ultrasonic-microwave synergistic extraction: mixing the S4 radix Millettiae Speciosae extract with water-saturated n-butanol at a mass volume g/mL ratio of 1:5, extracting in an ultrasonic and microwave synergistic extraction tank at 2000MHz and 8KW respectively at 60KHz and 2KW respectively at 50 deg.C for 60min, collecting the upper phase, concentrating, and precipitating to obtain radix Millettiae Speciosae total saponin.

Example 3

An ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root comprises the following steps:

s1, material treatment: cleaning and airing the beautiful millettia root medicinal material, conveying the medicinal material into a dryer, and continuously and dynamically drying the medicinal material by hot air at 50 ℃ until the moisture of the beautiful millettia root is lower than 10 percent to obtain dried beautiful millettia root;

s2, cold soaking: cutting the dried beautiful millettia root into segments, putting the segments into a dichloromethane solvent with the volume fraction of 80 percent for cold soaking, wherein the mass volume ratio g/mL of the dried beautiful millettia root to the dichloromethane solvent is 0.5: 3, stirring for 3 hours after primary cold soaking for 2 days, stirring for 1.5 hours after secondary cold soaking for 3 days, and filtering to obtain a beautiful millettia root cold soaking section;

s3, enzyme hydrolysis: according to the volume ratio of 1:8, mixing the yeast protease with distilled water to prepare a yeast protease solution, soaking the cold soaking section of the beautiful millettia root in the yeast protease solution, controlling the temperature to be 30-50 ℃, controlling the relative humidity to be 80% and carrying out pressurized hydrolysis for 3 hours under the pressure of 1.5 MPa;

s4, extracting an extract: adding an ether solvent into the millettia speciosa champ subjected to enzyme hydrolysis according to the mass volume ratio of g/mL of 1:5, stirring and reflux-extracting for 40min at 40 ℃, filtering, collecting residues for later use, and concentrating the filtrate under reduced pressure to obtain millettia speciosa champ extract;

s5, ultrasonic-microwave synergistic extraction: mixing the S4 radix Millettiae Speciosae extract with water-saturated n-butanol at a mass volume g/mL ratio of 1:4, putting into an ultrasonic and microwave synergistic extraction tank for extraction, wherein the microwave frequency is 1800MHz, the power is 6KW, the ultrasonic frequency is 50KHz, the power is 1.5KW, the synergistic extraction temperature is 45 ℃, the extraction time is 24min, collecting the upper phase, concentrating, and precipitating to obtain the radix Millettiae Speciosae total saponins.

Example 4

The difference between the embodiment and the embodiment 3 is that the mass-to-volume ratio g/mL of the radix millettiae speciosae dried by the S2 and the dichloromethane solvent is 0.2: 8.

example 5

The difference between the embodiment and the embodiment 3 is that the mass-to-volume ratio g/mL of the bovine macroergic force after S4 enzyme hydrolysis and the ether solvent is 1: 10.

Example 6

The present embodiment is different from embodiment 3 in that the microwave frequency in the S5 ultrasonic-microwave synergistic extraction is 1000MHz, and the power is 10 KW.

Comparative example 1

This comparative example differs from example 3 in that the dried beautiful millettia root was not cold-soaked with a methylene chloride solvent; in particular to an ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root, which comprises the following steps:

s1, material treatment: cleaning and airing the beautiful millettia root medicinal material, conveying the medicinal material into a dryer, and continuously and dynamically drying the medicinal material by hot air at 50 ℃ until the moisture of the beautiful millettia root is lower than 10 percent to obtain dried beautiful millettia root;

s2, enzyme hydrolysis: according to the volume ratio of 1:8, mixing the yeast protease with distilled water to prepare a yeast protease solution, soaking the dried radix millettiae speciosae in the yeast protease solution, controlling the temperature to be 30-50 ℃, controlling the relative humidity to be 80% and carrying out pressurized hydrolysis for 3 hours under the pressure of 1.5 MPa;

s3, extracting an extract: adding an ether solvent into the millettia speciosa champ subjected to enzyme hydrolysis according to the mass volume ratio of g/mL of 1:5, stirring and reflux-extracting for 40min at 40 ℃, filtering, collecting residues for later use, and concentrating the filtrate under reduced pressure to obtain millettia speciosa champ extract;

s4, ultrasonic-microwave synergistic extraction: mixing the S4 radix Millettiae Speciosae extract with water-saturated n-butanol at a mass volume g/mL ratio of 1:4, putting into an ultrasonic and microwave synergistic extraction tank for extraction, wherein the microwave frequency is 1800MHz, the power is 6KW, the ultrasonic frequency is 50KHz, the power is 1.5KW, the synergistic extraction temperature is 45 ℃, the extraction time is 24min, collecting the upper phase, concentrating, and precipitating to obtain the radix Millettiae Speciosae total saponins.

Comparative example 2

The comparative example differs from example 3 in that the Millettia speciosa champ cold-dip stage soaking is not enzymatically hydrolyzed; in particular to an ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root, which comprises the following steps:

s1, material treatment: cleaning and airing the beautiful millettia root medicinal material, conveying the medicinal material into a dryer, and continuously and dynamically drying the medicinal material by hot air at 50 ℃ until the moisture of the beautiful millettia root is lower than 10 percent to obtain dried beautiful millettia root;

s2, cold soaking: cutting the dried beautiful millettia root into segments, putting the segments into a dichloromethane solvent with the volume fraction of 80 percent for cold soaking, wherein the mass volume ratio g/mL of the dried beautiful millettia root to the dichloromethane solvent is 0.5: 3, primary cold soaking for 2 days, stirring for 3 hours, secondary cold soaking for 3 days, stirring for 1.5 hours, and filtering to obtain a beautiful millettia root cold soaking section;

s3, extracting an extract: adding an ether solvent into the cold soaking section of the beautiful millettia root according to the mass volume ratio g/mL of 1:5, stirring and reflux-extracting for 40min at 40 ℃, filtering, collecting residues for later use, and concentrating the filtrate under reduced pressure to obtain a beautiful millettia root extract;

s4, ultrasonic-microwave synergistic extraction: mixing the S4 radix Millettiae Speciosae extract with water-saturated n-butanol at a mass volume g/mL ratio of 1:4, putting into an ultrasonic and microwave synergistic extraction tank for extraction, wherein the microwave frequency is 1800MHz, the power is 6KW, the ultrasonic frequency is 50KHz, the power is 1.5KW, the synergistic extraction temperature is 45 ℃, the extraction time is 24min, collecting the upper phase, concentrating, and precipitating to obtain the radix Millettiae Speciosae total saponins.

Comparative example 3

This comparative example differs from example 3 in that the extraction is ultrasound assisted extraction; in particular to an ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root, which comprises the following steps:

s1, material treatment: cleaning and airing the beautiful millettia root medicinal material, conveying the medicinal material into a dryer, and continuously and dynamically drying the medicinal material by hot air at 50 ℃ until the moisture of the beautiful millettia root is lower than 10 percent to obtain dried beautiful millettia root;

s2, cold soaking: cutting the dried beautiful millettia root into segments, putting the segments into a dichloromethane solvent with the volume fraction of 80 percent for cold soaking, wherein the mass volume ratio g/mL of the dried beautiful millettia root to the dichloromethane solvent is 0.5: 3, primary cold soaking for 2 days, stirring for 3 hours, secondary cold soaking for 3 days, stirring for 1.5 hours, and filtering to obtain a beautiful millettia root cold soaking section;

s3, enzyme hydrolysis: according to the volume ratio of 1:8, mixing the yeast protease with distilled water to prepare a yeast protease solution, soaking the cold soaking section of the beautiful millettia root in the yeast protease solution, controlling the temperature to be 30-50 ℃, controlling the relative humidity to be 80% and carrying out pressurized hydrolysis for 3 hours under the pressure of 1.5 MPa;

s4, extracting an extract: adding an ether solvent into the millettia speciosa champ subjected to enzyme hydrolysis according to the mass volume ratio of g/mL of 1:5, stirring and reflux-extracting for 40min at 40 ℃, filtering, collecting residues for later use, and concentrating the filtrate under reduced pressure to obtain millettia speciosa champ extract;

s5, ultrasonic-assisted extraction: mixing the S4 radix Millettiae Speciosae extract with water-saturated n-butanol at a mass volume g/mL ratio of 1:4, extracting in an ultrasonic extraction tank at an ultrasonic frequency of 50KHz and a power of 1.5KW at 45 deg.C for 24min, collecting the upper phase, concentrating, and precipitating to obtain radix Millettiae Speciosae total saponin.

Comparative example 4

This comparative example differs from example 3 in that the extraction is microwave assisted extraction; in particular to an ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root, which comprises the following steps:

s1, material treatment: cleaning and airing the beautiful millettia root medicinal material, conveying the medicinal material into a dryer, and continuously and dynamically drying the medicinal material by hot air at 50 ℃ until the moisture of the beautiful millettia root is lower than 10 percent to obtain dried beautiful millettia root;

s2, cold soaking: cutting the dried beautiful millettia root into segments, putting the segments into a dichloromethane solvent with the volume fraction of 80 percent for cold soaking, wherein the mass volume ratio g/mL of the dried beautiful millettia root to the dichloromethane solvent is 0.5: 3, primary cold soaking for 2 days, stirring for 3 hours, secondary cold soaking for 3 days, stirring for 1.5 hours, and filtering to obtain a beautiful millettia root cold soaking section;

s3, enzyme hydrolysis: according to the volume ratio of 1:8, mixing the yeast protease with distilled water to prepare a yeast protease solution, soaking the cold soaking section of the beautiful millettia root in the yeast protease solution, controlling the temperature to be 30-50 ℃, controlling the relative humidity to be 80% and carrying out pressurized hydrolysis for 3 hours under the pressure of 1.5 MPa;

s4, extracting an extract: adding an ether solvent into the millettia speciosa champ subjected to enzyme hydrolysis according to the mass volume ratio of g/mL of 1:5, stirring and reflux-extracting for 40min at 40 ℃, filtering, collecting residues for later use, and concentrating the filtrate under reduced pressure to obtain millettia speciosa champ extract;

s5, microwave-assisted extraction: mixing the S4 radix Millettiae Speciosae extract with water-saturated n-butanol at a mass volume g/mL ratio of 1:4, extracting in an ultrasonic and microwave synergistic extraction tank at 1800MHz and 6KW power at 45 deg.C for 24min, collecting the upper phase, concentrating, and precipitating to obtain radix Millettiae Speciosae total saponin.

First, result determination

The millettia speciosa champ total saponins extracted in the examples 1 to 6 and the comparative examples 1 to 4 are analyzed by a High Performance Liquid Chromatography (HPLC), and the conditions are as follows:

(1) a chromatographic column: sephadex LH;

(2) mobile phase A: water; mobile phase B: acetonitrile;

(3) flow rate: 1 mL/min;

(4) detection wavelength: 545 nm;

(5) sample introduction volume: 20 mL.

Calculating total saponin content according to total volume, integrated peak area and weight of HPLC chromatogram analysis, calculating total saponin yield,

the yield of the total saponins is M/Mx 100%, M is the mass of the total saponins extracted from the beautiful millettia root, and M is the mass of the total saponins extracted from the beautiful millettia root;

the results are as follows:

	total saponin content (%)	Total saponins yield (%)
			Example 1	97.1	98.0
Example 2	97.3	98.2
			Example 3	98.8	98.3
Example 4	92.6	96.5
			Example 5	93.9	95.8
Example 6	91.2	96.0
			Comparative example 1	87.5	90.1
Comparative example 2	86.1	89.2
			Comparative example 3	87.2	88.3
Comparative example 4	82.9	87.9

The results show that the extraction method of the invention removes fat-soluble impurities and water-soluble impurities, and efficiently separates substances such as saponin and structural analogues thereof, so as to realize the preparation of high purity of total saponin and improve the total yield of saponin; compared with the comparative example 1, after the beautiful millettia root is subjected to cold soaking by using a dichloromethane solvent, the adsorption force between chemical components and histiocytes in the beautiful millettia root is overcome, and the effective components in the beautiful millettia root can be extracted; compared with the comparative example 2, the millettia speciosa champ cold-soaking section is soaked and hydrolyzed by enzyme, and then the saponin is hydrolyzed to obtain sapogenin; compared with comparative examples 3 and 4, ultrasonic-microwave synergistic extraction is adopted, the frequency and power are controlled, a better auxiliary extraction effect is achieved at the common extraction temperature, the problem of saponin foaming in the extraction process is inhibited, impurities are reduced, and the purity of saponin is improved.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.