阅读说明：本技术 用于鉴定抗原特异性t细胞的组合物和方法 (Compositions and methods for identifying antigen-specific T cells ) 是由 彭松明 博伊·布莱恩特·夸克 安朵 罗伯特·包晓研 亚历克西斯·弗兰佐索夫 芭芭拉·森尼诺 于 2020-02-12 设计创作，主要内容包括：本文公开了抗原肽-MHC复合物,称为comPACT多肽和comPACT多核苷酸,以及产生这种复合物的方法。本文还公开了产生comPACT多核苷酸和多肽的文库的方法,以及它们在以高精度捕获癌症新表位反应性T细胞中的示例性用途。提供了用于检测具有改进的灵敏度和特异性的新抗原特异性T细胞的双颗粒检测方法。还提供了分离的T细胞的信噪比分析,其用于检测具有改进的T细胞的新抗原特异性T细胞。(Disclosed herein are antigenic peptide-MHC complexes, referred to as comPACT polypeptides and comPACT polynucleotides, and methods of producing such complexes. Also disclosed herein are methods of generating libraries of comPACT polynucleotides and polypeptides, and their exemplary uses in capturing cancer neo-epitope reactive T cells with high accuracy. A dual particle detection method for detecting neoantigen-specific T cells with improved sensitivity and specificity is provided. Also provided is a signal to noise ratio assay of isolated T cells for detecting neoantigen-specific T cells with improved T cells.)