阅读说明：本技术 在体膜片钳与光纤记录结合的神经信号记录方法及系统 (Neural signal recording method and system combining on-body membrane forceps and optical fiber recording ) 是由 赵文杰 蔚鹏飞 王立平 于 2020-09-18 设计创作，主要内容包括：本发明提供了一种在体膜片钳与光纤记录结合的神经信号记录方法及系统,其中神经信号记录系统包括荧光信号记录单元、激发单元、膜片钳记录单元和控制与处理单元,膜片钳记录单元包括电生理数模转换器。荧光信号记录单元记录荧光信号,膜片钳记录单元记录神经电信号,荧光信号和神经电信号在电生理数模转换器中同步。本发明的系统能够同时反映出脉冲放电等膜电位的变化和相关联环路的神经化学信号变化情况,在神经信号记录的维度、形式以及获得数据的丰富性上有质的提升。(The invention provides a neural signal recording method and system combining on-body membrane clamp and optical fiber recording, wherein the neural signal recording system comprises a fluorescence signal recording unit, an excitation unit, a patch clamp recording unit and a control and processing unit, and the patch clamp recording unit comprises an electrophysiological digital-to-analog converter. The fluorescence signal recording unit records a fluorescence signal, the patch clamp recording unit records a nerve electric signal, and the fluorescence signal and the nerve electric signal are synchronized in the electrophysiological digital-to-analog converter. The system can reflect the change of membrane potential such as pulse discharge and the like and the change condition of the neurochemical signals of the associated loop at the same time, and the dimensionality and the form of the record of the neurochemical signals and the richness of the obtained data are improved substantially.)

1. A neural signal recording system combining on-body membrane clamp and optical fiber recording is characterized by comprising a fluorescence signal recording unit, an excitation unit, a patch clamp recording unit and a control and processing unit;

the patch clamp recording unit comprises an electrophysiological digital-to-analog converter and is used for collecting a neural electric signal;

the patch clamp recording unit is connected with the fluorescence signal recording unit through the electrophysiological digital-to-analog converter and is used for receiving the fluorescence signal acquired by the fluorescence recording unit;

the patch clamp recording unit is connected with the excitation unit through the electrophysiological digital-to-analog converter and is used for transmitting a control signal to the excitation unit;

the electrophysiological digital-to-analog converter synchronizes signals collected by the fluorescence signal recording unit and the patch clamp recording unit, is connected with the control and processing unit, and receives a control signal from the control and processing unit.

2. The system for recording the neural signals by combining the on-body membrane forceps with the optical fiber recording as claimed in claim 1, wherein the excitation unit comprises a laser light source, the fluorescence signal recording unit further comprises an optical fiber insertion pin, and the optical fiber insertion pin is further connected with the laser light source.

3. The system for recording the neural signals by combining the on-body membrane forceps and the optical fiber records as claimed in claim 2, wherein the fluorescence signal recording unit further comprises an optical fiber recording host and an optical fiber jumper, one end of the optical fiber jumper is connected with the optical fiber insertion pin, and the other end of the optical fiber jumper is connected with the optical fiber recording host.

4. The system for recording the neural signals by combining the on-body membrane forceps with the optical fiber records according to claim 3, wherein the optical fiber recording host comprises a light path front end, an optical signal collector and an optical signal converter, the light path front end, the optical signal collector and the optical signal converter are sequentially connected, and the light path front end is connected with the optical fiber jumper.

5. The system for recording the neural signals by combining the on-body membrane clamp and the optical fiber record according to claim 1, wherein the patch clamp recording unit further comprises a signal recording probe and a neural signal collector, and the signal recording probe, the neural signal collector and the electrophysiological digital-to-analog converter are sequentially connected.

6. The system for recording the neural signals by combining the on-body membrane forceps with the optical fiber records according to claim 4, wherein the optical fiber recording host further comprises an optical signal amplifier, and the optical signal amplifier is located between the optical signal collector and the optical signal converter and is respectively connected with the optical signal converter and the optical signal collector.

7. The system for recording the neural signals by combining the on-body membrane forceps with the optical fiber records as claimed in claim 5, further comprising an electrical signal amplifier, wherein the electrical signal amplifier is positioned between the signal recording probe and the neural signal collector, and the electrical signal amplifier is connected with the signal recording probe and the neural signal collector.

8. The system for recording nerve signals by combining on-body membrane forceps with optical fiber recording according to claim 2, wherein the laser light source is a bicolor laser light source.

9. The system for recording nerve signals by combining on-body membrane forceps with optical fiber recording as claimed in claim 2, wherein the bottom surface of the optical fiber insertion needle is a horizontal bottom surface or an inclined bottom surface.

10. Method for using a system according to any of claims 1-9, characterized in that it comprises the following steps:

step S1, inserting the optical fiber pin of the fluorescence signal recording unit into the target brain area, and inserting the signal recording probe of the patch clamp recording unit into the same target brain area in an inclined manner;

step S2, using the excitation unit to send out excitation signal; the fluorescence signal recording unit collects fluorescence signals; the patch clamp recording unit collects a neural electric signal;

step S3, transmitting the fluorescence signal and the neuroelectric signal into the electrophysiological digital-to-analog converter for synchronization;

step S4, storing the data in the electrophysiological digital-to-analog converter in the control and processing unit.

11. The method for recording nerve signals by combining in-vivo membrane clamp and optical fiber recording as claimed in claim 10, wherein in step S1, the signal recording probe is inserted into the same target brain region at a distance of 1.5mm from the optical fiber insertion needle and inclined at 45 ° -60 ° relative to the optical fiber insertion needle.

Technical Field

The invention relates to the technical field of neural signal recording, in particular to a neural signal recording method and system combining on-body membrane forceps and optical fiber recording, which can simultaneously record fluorescence signals and electric signals.

Background

The study of the nervous system of conscious animals is an important topic in contemporary neuroscience research. With the continuous and intensive neuroscience research, the manner and method of neural signal recording is undergoing continuous innovation. The current recording method of the neural signals mainly takes fluorescent signal recording and electric signal recording as main parts. In the prior art, a specific in-vivo conscious animal experiment generally adopts a single recording method, namely only a fluorescence signal or only an electric signal is recorded.

In the prior art, when an optical fiber recording system is used for recording a fluorescence signal, the fluorescence signal is collected through an optical fiber, the collected fluorescence signal is converted into an electric signal through an optical signal converter, and then the electric signal is transmitted to the recording system through a data acquisition card, so that the purpose of observing the fluorescence activity of a group of nerve cells in a brain area to be researched in real time is achieved. However, the optical fiber recording method cannot detect the activity of a single cell, only can detect fluorescence signals of group neurons near the tip of the optical fiber probe, and the dimension and the fidelity of the neural signals presented by the optical signals are restricted by the problems that the fluorescence signals are weak, the sampling frequency of a recording optical signal system is low, the signal to noise ratio is low, and the like.

The electrical signals generally reflect changes in membrane potential of the neuron, including action potentials, subthreshold potentials, and field potentials. The patch clamp recording is a method for recording membrane current or membrane potential of a cell patch adsorbed by an electrode tip, wherein a glass microelectrode suction tube is used for sucking and sealing cell membranes which only contain 1-3 ion channels and have the area of several square microns by negative pressure, the membrane covered by the electrode tip is electrically isolated from other parts due to the high-resistance sealing of the electrode tip and the cell membranes, the small cell membranes are sucked and broken by the negative pressure, so that the inside of a cell is communicated with the inside of the glass microelectrode, a whole cell recording mode is further formed, and the current intensity/voltage change can be measured by using a sensitive current/voltage monitoring probe through the opening of the channel. However, the body membrane clamp electrophysiological method has the problems of limited cell number recorded in a single experiment, single recorded signal, difficulty in obtaining the source of the nerve electrical signal, and the like.

In summary, the above existing methods for recording neural signals have limitations, which limit further mining and cognition of the neural signals by scientific experiments, and thus, improvements to the existing technologies are needed.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides a neural signal recording method and system combining on-body membrane clamp and optical fiber recording, and a fluorescence signal recording method and a patch clamp recording method are integrated in a set of system through optimization design so as to solve the problem of monotonous in-vivo experiment recording method.

In order to achieve the purpose, the invention provides a nerve signal recording system combining body membrane clamp and optical fiber recording, which comprises a fluorescence signal recording unit, an excitation unit, a patch clamp recording unit and a control and processing unit;

the patch clamp recording unit comprises an electrophysiological digital-to-analog converter and is used for collecting a neural electric signal;

the patch clamp recording unit is connected with the fluorescence signal recording unit through the electrophysiological digital-to-analog converter and is used for receiving an electric signal converted from the fluorescence signal acquired by the fluorescence recording unit;

the patch clamp recording unit is connected with the excitation unit through the electrophysiological digital-to-analog converter and is used for transmitting a control signal to the excitation unit;

the electrophysiological digital-to-analog converter synchronizes signals collected by the fluorescence signal recording unit and the patch clamp recording unit, is connected with the control and processing unit, and receives a control signal from the control and processing unit.

Further, the excitation unit includes laser light source, fluorescence signal record unit still includes the optic fibre contact pin, the optic fibre contact pin still connects laser light source, optic fibre contact pin both can transmit the exciting light, can gather fluorescence signal again, have realized the integration of system, have not only reduced the cost of system, can not occupy too much space in addition, make things convenient for the experimenter to operate.

Furthermore, the fluorescence signal recording unit also comprises an optical fiber recording host and an optical fiber jumper, wherein one end of the optical fiber jumper is connected with the optical fiber contact pin, and the other end of the optical fiber jumper is connected with the optical fiber recording host.

Furthermore, the optical fiber recording host comprises a light path front end, an optical signal collector and an optical signal converter, wherein the light path front end, the optical signal collector and the optical signal converter are sequentially connected, and the light path front end is connected with the optical fiber jumper.

Furthermore, the patch clamp recording unit further comprises a signal recording probe and a neural signal collector, and the signal recording probe, the neural signal collector and the electrophysiological digital-to-analog converter are sequentially connected through a BNC cable.

Further, the optical fiber recording host further comprises an optical signal amplifier, the optical signal amplifier is located between the optical signal collector and the optical signal converter and is respectively connected with the optical signal converter and the optical signal collector, and the optical signal amplifier amplifies the received relatively weak fluorescent signal so as to avoid attenuation of the signal to zero in the transmission process.

The electric signal amplifier is connected with the signal recording probe and the nerve signal collector through the BNC cable, and amplifies the recorded tiny electric signals, so that the attenuation degree of the electric signals in the transmission process is reduced.

Furthermore, the bottom surface of the optical fiber inserting needle is a horizontal bottom surface or an inclined bottom surface, and compared with a plane, the contact area between the inclined bottom surface and the target brain area is larger for the optical fiber inserting needle with the same diameter, so that the receiving efficiency of optical signals is higher.

Furthermore, the laser light source is a bicolor laser light source, and the fluorescence brightness is not influenced by the activity intensity of the measured neuron, so that the laser light source can be used as a contrast group to eliminate signal artifacts caused by optical fiber winding and violent movement of the experimental animal, the self contrast of the experimental animal is realized, and the collection efficiency of fluorescence signals can be improved by increasing the numerical aperture of the multimode optical fiber.

A nerve signal recording method combining body membrane forceps and optical fiber recording comprises the following steps:

step S1, inserting the optical fiber pin of the fluorescence signal recording unit into the target brain area, and inserting the signal recording probe of the patch clamp recording unit into the same target brain area in an inclined manner;

step S2, using the excitation unit to send out excitation signal; the fluorescence signal recording unit collects fluorescence signals; the patch clamp recording unit collects a neural electric signal;

step S3, transmitting the fluorescence signal and the neuroelectric signal into the electrophysiological digital-to-analog converter for synchronization;

step S4, storing the data in the electrophysiological digital-to-analog converter in the control and processing unit.

Further, in step S1, the signal recording probe is inserted into the same target brain region at a position 1.5mm away from the optical fiber stub and inclined at 45 ° -60 ° relative to the optical fiber stub.

The invention has at least the following beneficial effects: the fluorescence signal recording unit records fluorescence signals, the patch clamp recording unit records nerve electrical signals, and the fluorescence signals and the nerve electrical signals are synchronized through the electrophysiological digital-to-analog converter. The system can reflect the change of membrane potential such as pulse discharge and the like and the change condition of the neurochemical signals of the associated loop at the same time, and improves the dimensionality and the form of the record of the neurochemical signals and the richness of the obtained data qualitatively.

Drawings

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

Fig. 1 is a block diagram of a nerve signal recording system combining body membrane forceps and optical fiber recording according to the present invention.

Fig. 2 is a diagram of the state of the nerve signal recording system combining the body membrane forceps and the optical fiber recording according to the invention.

Fig. 3 is a flow chart of the application of the neural signal recording method combining the body membrane forceps and the optical fiber recording according to the invention.

In the figure: the system comprises a 1-fluorescence signal recording unit, 11-optical fiber insertion pins, 12-optical fiber jumper wires, 13-optical fiber recording host, a 2-excitation unit, a 21-laser light source, a 3-patch clamp recording unit, a 31-signal recording probe, a 32-electric signal amplifier, a 33-neural signal collector, a 34-electrophysiological digital-to-analog converter, a 35-BNC cable, a 4-control and processing unit and an A-skull.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. The invention is mainly directed to the recording of nerve signals in the deep and subcortical brain regions of the cerebral cortex, and the embodiment takes a small animal as an example, but the invention is also applicable to the middle and lower cerebral regions of the cerebral cortex of a primate.

In general, due to the limitations of conventional experimental techniques and conditions, a particular in vivo experiment employs a single recording method, i.e., recording only fluorescent signals or only electrical signals. In the prior art, an optical fiber recording method is usually used for recording a fluorescence signal, however, the optical fiber recording method cannot detect the activity of a single cell, only group neuron fluorescence signals near the tip of an optical fiber probe can be detected, and the dimension and fidelity of the neural signals which can be presented by the optical signals are restricted by the problems of weak fluorescence signals, low sampling frequency of a recording optical signal system, low signal-to-noise ratio and the like; in the prior art, the electrical signal is recorded by a patch clamp electrophysiological method, however, in the patch clamp electrophysiological method, only the electrical signal of a single cell can be detected in a single experiment, and the recorded signal is single, so that the source of the electrical signal affecting the nerve is difficult to know.

The invention provides a nerve signal recording system combining body membrane forceps and optical fiber recording, and aims to solve the problem of limitation in the prior art. The neural cells studied in the examples of the present invention have been previously expressed with fluorescent molecules and light-activated ion channels. As shown in fig. 1, the system of the embodiment of the present invention is a block diagram, and includes a fluorescence signal recording unit 1, an excitation unit 2, a patch clamp recording unit 3, and a control and processing unit 4. The control and processing unit 4 is connected with the patch-clamp recording unit 3, and the patch-clamp recording unit 3 is respectively connected with the excitation unit 2 and the fluorescence signal recording unit 1. The excitation unit 2 mainly activates the nerve cells expressing the light-activated ion channels by laser, so as to realize the regulation and control of excitation or inhibition of the nerve cells, or make the nerve cells expressing fluorescent molecules show dynamic change of fluorescence intensity under the excitation light. The fluorescence signal recording unit 1 is mainly used for collecting fluorescence signals of the nerve cells expressing fluorescence molecules which are subjected to fluorescence change under excitation light, and the fluorescence molecules can be expressed in specific brain regions and specific types of neurons in specific and non-specific brain regions. The patch clamp recording unit 3 is used for collecting the potential signal of the stimulated nerve cell membrane and integrating the potential signal with the fluorescence signal collected by the fluorescence signal recording unit 1. The control and processing unit 4 plays a role of controlling, for example, setting the frequency, intensity and time of the light stimulation laser, and the intensity and gain of the excitation light for exciting the fluorescence signal. In addition to this, the control and processing unit 4 also has the function of storing data. Under the control of the control and processing unit 4, the excitation unit 2 activates the nerve cells expressing the light-activated ion channels through laser, or the nerve cells expressing fluorescent molecules generate fluorescence change under the action of excitation light, and the fluorescence signal recording unit 1 collects fluorescence signals and guides the collected fluorescence signals into the patch clamp recording unit 3. The patch clamp recording unit 3 records the membrane potential or current of a single nerve cell in the same brain area, simultaneously receives the fluorescence signal acquired by the fluorescence signal recording unit 1, records the fluorescence signal and the electric signal, then integrates the fluorescence signal and the electric signal acquired at the same time, ensures the synchronism of signal acquisition, and finally stores data on the control and processing unit 4, thereby realizing the purpose of simultaneously recording the fluorescence signal and the nerve electric signal in the same brain area by combining the optical fiber recording and patch clamp recording methods.

Specifically, the patch clamp recording unit 3 includes a signal recording probe 31, an electrical signal amplifier 32, a neural signal collector 33, an electrophysiological digital-to-analog converter 34, and a BNC cable 35. The signal recording probe 31, the neural signal collector 33, the electric signal amplifier 32 and the electrophysiological digital-to-analog converter 34 are connected in sequence by a BNC cable 35. The signal recording probe 31 enters the target brain area or the nuclear mass, for example, the signal recording probe 31 is pushed into the target brain area or the nuclear mass by an ultramicro propulsion device, and is adsorbed to a single nerve cell in the target brain area or the nuclear mass, and then the adsorbed cell membrane is broken by negative pressure, so that the current or potential change of the nerve cell is observed in a full cell mode, and the patch clamp recording unit 3 can record the membrane potential or current of the single nerve cell. The electrical signal recorded by the signal recording probe 31 is collected by the neural signal collector 33, amplified by the electrical signal amplifier 32 to avoid signal attenuation during transmission, and then transmitted to the electrophysiological d/a converter 34.

The fluorescence signal recording unit 1 comprises an optical fiber pin 11, an optical fiber jumper 12 and an optical fiber recording host 13. One end of the optical fiber jumper 12 is connected with the optical fiber pin 11, and the other end is connected with the optical fiber recording host 13. The optical fiber recording host 13 includes an optical path front end, an optical signal collector, an optical signal amplifier, and an optical signal converter, which are connected in sequence. The front end of the optical path is connected with an optical fiber jumper 12 for leading in a fluorescence signal. The excitation unit 2 comprises a laser light source 21, and the laser light source 21 is connected with the optical fiber pin 11 of the fluorescence signal recording unit 1 through an optical fiber jumper 12. The optical fiber jumper 12 connects the optical fiber stub 11 and the laser light source 21. The optical fiber pins 11 are implanted into a target brain area or nucleus of the animal brain, and the optical fiber pins 11 are implanted into the target brain area or nucleus of the animal brain through an ultramicrotome. The laser light source 21 emits laser light to activate nerve cells expressing light-activated ion channels, or nerve cells expressing fluorescent molecules undergo fluorescence change under the action of excitation light. In the system of the invention, the optical fiber contact pin 11 can not only transmit exciting light, but also collect fluorescence signals, thereby realizing the integration of the system, not only reducing the cost of the system, but also ensuring that the system does not occupy too much space, and being convenient for experimenters to operate. Because the optical signal is lost in the transmission process, when the optical fiber contact pin 11 collects the fluorescence signal, the fluorescence signal enters the optical fiber recording host 13 through the optical fiber jumper 12, then the fluorescence signal is transmitted to the optical signal amplifier through the optical signal collector to be amplified, and the amplified signal is converted into an electrical signal through the optical signal converter and then is output to the electrophysiological digital-to-analog converter 34.

Further, the fiber recording host 13 may configure a corresponding laser with a specific wavelength or an LED light source to generate laser light according to the requirement. Preferably, in this embodiment, the excitation unit 2 uses a two-color laser light source, excitation lights of two colors are coupled in the same optical fiber, and two fluorescence signals generated by excitation are transmitted and respectively collected through an optical fiber jumper. In the fluorescent signals of two color wave bands, one path of fluorescent signal is a sensitive fluorescent signal excited by blue light, the other path of fluorescent signal is a fluorescent signal excited by red light, and the fluorescent brightness of the fluorescent signal is not influenced by the activity intensity of the measured neuron, so that the fluorescent signal can be used as a control group to eliminate signal artifacts caused by optical fiber winding and violent movement of an experimental animal, the self-control of the experimental animal is realized, and the collection efficiency of the fluorescent signal can be improved by increasing the numerical aperture of the multimode optical fiber.

In the invention, the electrophysiological digital-to-analog converter 34 collects the electrical signal converted from the fluorescence signal collected by the fluorescence recording unit 1 and the neural electrical signal recorded by the patch clamp recording unit 3, which enter the electrophysiological digital-to-analog converter 34 simultaneously, so as to realize the synchronous recording of different signals. The system can reflect the change of membrane potential such as pulse discharge and the like and the change condition of the neurochemical signals of the associated loop at the same time, and improves the dimensionality and the form of the record of the neurochemical signals and the richness of the obtained data qualitatively.

The control and processing unit 4 comprises a control module and a data storage module. The control module is used for sending an instruction to the patch clamp recording unit 3, and then controlling the excitation unit 2 through the patch clamp recording unit 3, for example, setting the frequency, intensity and time of the light stimulation laser, and the excitation light intensity, gain and other indexes of the excited fluorescence signal, and simultaneously controlling the fluorescence signal recording unit 1 through the patch clamp recording unit 3. The data storage module is used for storing the data recorded and integrated in the electrophysiological digital-to-analog converter 34.

In the prior art, the tail end of the optical fiber insertion pin 11 is a plane, the contact area with a target brain area is limited, the receiving efficiency of optical signals is low, and the acquisition of the optical signals by the optical fiber insertion pin 11 is limited. In this embodiment, the optical fiber stub 11 has an inclined bottom surface, and for the optical fiber stub with the same diameter, compared with a plane, the contact area between the inclined bottom surface and the target brain area is larger, and the receiving efficiency of the optical signal is higher. Further, the optical fiber stub 11 may be replaced with a micro glass prism.

If the patch clamp recording unit 3 only records the discharge signal or the field potential of the neuron, the signal recording probe 31 can be a multi-channel electrode; the patch-clamp recording unit 3 the signal recording probe 31 may be a glass microelectrode if it records the electrical discharge of a single neuron. Preferably, the glass microelectrode is selected as the signal recording probe 31 in the present invention.

The invention also provides a nerve signal recording method combining body membrane forceps and optical fiber recording, which comprises the following steps:

step S1, inserting the optical fiber insertion needle 11 into the target brain area perpendicular to the surface of the small animal skull A, and inserting the glass microelectrode into the same target brain area at a certain inclination angle;

fig. 2 is a diagram showing a state of use of the system of the present invention. For small animals, such as mice, the surface area of the skull A is relatively small, and after the head fixation operation, part of the surface area of the skull A is covered by the fixative, so that the operable space of the skull A is reduced. The fluorescence signal recording unit 1, the excitation unit 2 and the patch clamp recording unit 3 act on the same brain region, and have a competitive relationship in space. The optical fiber stub 11 is composed of a length of high NA optical fiber and a corresponding opaque ceramic sleeve, and considering the limited operation space of the small animal skull A, the ceramic sleeve with the diameter not more than 1.25mm and the optical fiber stub 11 with the diameter not more than 200 μm are selected. The diameter of the optical fiber insertion pin 11 is reduced as much as possible on the premise of ensuring the fluorescent signal collection efficiency. The patch clamp recording unit 3 generally adopts a glass microelectrode for recording, the diameter of the tip of the glass microelectrode is less than 4 μm, compared with the optical fiber pin 11, the glass microelectrode has very small damage to brain tissue and is suitable for being propelled in the brain tissue for a long distance, and the optical fiber pin 11 has a larger diameter and relatively obvious damage to the brain tissue, and needs to reach a target brain area by using the shortest distance. Thus, in a specific implementation, fiber stub 11 is inserted into the targeted brain region perpendicular to the surface of skull A of the small animal. When the optical fiber insertion needle 11 is inserted into a target brain area, the glass microelectrode is inserted into the same target brain area at an inclination angle of 45-60 degrees at a position which is about 1.5mm away from the ceramic sleeve, and at the moment, the optical fiber insertion needle 11 and the glass microelectrode are compatible in the same space. Furthermore, according to the area of the operable area of the small animal skull A and the position of the target brain area, at least one glass microelectrode can be matched with one optical fiber inserting needle 11.

Step S2, using the laser source 21 to emit laser to activate the nerve cell expressing the light activated ion channel; the optical fiber contact pin 11 conducts the fluorescent signal, then transmits the fluorescent signal to the optical fiber recording host 13 through the optical fiber jumper, amplifies the signal through the optical signal amplifier 13 in the optical fiber recording host 13, and converts the signal into an electric signal through the optical signal converter 14; the glass microelectrode records the membrane potential or current of a single nerve cell, and the recorded electric signal is amplified by an electric signal amplifier 32 and then transmitted to the nerve signal collector 3;

step S3, the signals collected by the fluorescence signal recording unit 1 and the signals collected by the patch clamp recording unit 3 are transmitted to the electrophysiological D/A converter 34 for synchronization;

in step S4, the data in the electrophysiological d/a converter 34 is imported into the control and processing unit 4 for storage.

Therefore, the invention provides a neural signal recording method and system combining on-body membrane clamp and optical fiber recording, wherein the neural signal recording system comprises a fluorescence signal recording unit, an excitation unit, a patch clamp recording unit and a control and processing unit, and the patch clamp recording unit comprises an electrophysiological digital-to-analog converter. The laser unit and the fluorescent signal recording unit in the system share one optical fiber contact pin, wherein the optical fiber contact pin can transmit exciting light and collect fluorescent signals. The fluorescence signal recording unit records a fluorescence signal, the patch clamp recording unit records a nerve electric signal, and the fluorescence signal and the nerve electric signal are synchronized in the electrophysiological digital-to-analog converter. The system can reflect the change of membrane potential such as pulse discharge and the like and the change condition of the neurochemical signals of the associated loop at the same time, and the dimensionality and the form of the record of the neurochemical signals and the richness of the obtained data are improved substantially.

The above description is only exemplary of the present invention and should not be taken as limiting the invention, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.