使用光致发光无机纳米颗粒的毛细作用测试
阅读说明:本技术 使用光致发光无机纳米颗粒的毛细作用测试 (Capillary action test using photoluminescent inorganic nanoparticles ) 是由 帕斯卡尔·普瑞拉 马克西米利安·里奇伊 塞德里克·布兹格 安蒂戈尼·亚历桑德罗 蒂埃里·加库 于 2019-07-17 设计创作,主要内容包括:本发明涉及一种用于通过毛细作用测试来检测和/或定量液体样品中感兴趣的生物或化学物质的体外方法,所述毛细作用测试使用式A-(1-)-xLn-xVO-(4(1-y))(PO-4)-y(II)的光致发光无机纳米颗粒作为探针,其中Ln选自铕(Eu)、镝(Dy)、钐(Sm)、钕(Nd)、铒(Er)、镱(Yb)、铥(Tm)、镨(Pr)、钬(Ho)及其混合物;A选自钇(Y)、钆(Gd)、镧(La)、镥(Lu)及其混合物;0<x<1;且0≤y<1,所述方法进行通过在小于或等于320nm的波长下激发基质,在单光子吸收之后的所述纳米颗粒的发光检测,所述纳米颗粒具有的发射寿命短于100ms。本发明还涉及一种包括上述纳米颗粒作为探针的毛细作用测试以及这样的方法用于体外诊断目的的用途。(The present invention relates to an in vitro method for detecting and/or quantifying a biological or chemical substance of interest in a liquid sample by means of a capillary action test using formula A 1‑ x Ln x VO 4(1‑y) (PO 4 ) y (II) as a probe,wherein Ln is selected from europium (Eu), dysprosium (Dy), samarium (Sm), neodymium (Nd), erbium (Er), ytterbium (Yb), thulium (Tm), praseodymium (Pr), holmium (Ho) and mixtures thereof; a is selected from yttrium (Y), gadolinium (Gd), lanthanum (La), lutetium (Lu) and mixtures thereof; 0<x<1; and y is not less than 0<The method performs luminescence detection of the nanoparticles after single photon absorption by excitation of a matrix at a wavelength less than or equal to 320nm, the nanoparticles having an emission lifetime shorter than 100 ms. The invention also relates to a capillary test comprising the above-described nanoparticles as probes and to the use of such a method for in vitro diagnostic purposes.)