阅读说明：本技术 一种含有干细胞提取液的代谢和免疫增强及改善亚健康制剂 (A preparation containing stem cell extractive solution for improving metabolism and immunity and improving sub-health ) 是由 杨水祥 于 2021-07-09 设计创作，主要内容包括：本发明公开了一种含有干细胞提取液的代谢和免疫增强及改善亚健康制剂。所述制剂包括以下原料：干细胞提取液冻干粉10-250mg,β烟酰胺单核苷酸50-650mg,辅酶Q1010-120mg,黄芪多糖1-8g,灵芝提取物1-8g,人参皂苷A210-240mg,党参提取物1-5g,冬虫夏草提取物1-3g,褪黑素1-20mg,红花提取物1-5g,石斛提取物1-5g和表没食子儿茶素没食子酸酯1-5g,其余为去离子水。该制剂中的干细胞提取液含有各种生长因子及基因调控因子,能促进组织细胞重编程和逆分化,促进组织干细胞和骨髓增生及免疫活性增强,防衰老抗疲劳,改善睡眠,改善亚健康状态。(The invention discloses a preparation containing stem cell extract for enhancing metabolism and immunity and improving sub-health. The preparation comprises the following raw materials: 10-250mg of dry cell extract freeze-dried powder, 50-650mg of beta nicotinamide mononucleotide, 1010-120mg of coenzyme Q, 1-8g of astragalus polysaccharide, 1-8g of lucid ganoderma extract, 210-240mg of ginsenoside A, 1-5g of codonopsis pilosula extract, 1-3g of cordyceps sinensis extract, 1-20mg of melatonin, 1-5g of safflower extract, 1-5g of dendrobe extract and 1-5g of epigallocatechin gallate, and the balance of deionized water. The stem cell extractive solution in the preparation contains various growth factors and gene regulatory factors, and has effects of promoting tissue cell reprogramming and retrodifferentiation, promoting tissue stem cell and bone marrow hyperplasia, enhancing immunity, resisting aging and fatigue, improving sleep, and improving sub-health state.)

1. A preparation containing stem cell extract for enhancing metabolism and immunity and improving sub-health is characterized in that each 100mL of immunopotentiator comprises the following raw materials: 10-250mg of dry cell extract freeze-dried powder, 50-650mg of beta nicotinamide mononucleotide, 1010-120mg of coenzyme Q, 1-8g of astragalus polysaccharide, 1-8g of lucid ganoderma extract, 210-240mg of ginsenoside A, 1-5g of codonopsis pilosula extract, 1-3g of cordyceps sinensis extract, 1-20mg of melatonin, 1-5g of safflower extract, 1-5g of dendrobe extract and 1-5g of epigallocatechin gallate, and the balance of deionized water.

2. The agent for enhancing metabolism and immunity and improving sub-health as set forth in claim 1, wherein the stem cell extract is an extract of a jelly-plant stem cell.

3. The agent for enhancing metabolism and immunity and improving sub-health as claimed in claim 2, wherein the extract solution of the jelly-fish stem cell is obtained by the steps of:

(1) cleanly breeding live hydranth of young junglenian jellyfish for 24-48 hours, repeatedly washing with sterile normal saline, sterilizing with 70% alcohol, peeling and removing tentacles under the sterile condition of 4 ℃, crushing and filtering, adding a cell separation digestive juice and a buffer culture solution to prepare a single cell suspension, centrifugally washing with normal saline for 3 times, adding a proper amount of culture medium, incubating with a dry cell marker antibody with a selective marker in a 37 ℃ carbon dioxide incubator for 45 minutes, and carrying out ice bath at 4 ℃ overnight;

(2) separating the stem cells of the cell suspension with the stem cell markers by using a flow cytometer, collecting the stem cells, centrifugally washing the stem cells for three times under the aseptic condition of 4 ℃, removing markers, and adding a buffer solution to prepare a cell suspension;

(3) adding the cell suspension into precooled buffer solution, ultrasonically breaking cells under aseptic condition, centrifuging at 4 ℃, collecting supernatant, measuring protein concentration, and preparing the suspension containing 1-2 × 10 proteins per ml⁸Stem cell extracts of individual cells;

(4) mixing the obtained stem cell extract with albumin, physiological saline mixture and adjuvants.

4. The agent for enhancing metabolism and immunity and improving sub-health, which comprises the extract of stem cells as set forth in claim 1, wherein the extract of Ganoderma lucidum is obtained by the steps of: extracting Ganoderma in water at 90-100 deg.C to obtain extractive solution, concentrating the extractive solution, adding anhydrous ethanol, standing for 24-48 hr, and freeze drying to obtain Ganoderma extract.

5. The agent for enhancing metabolism and immunity and improving sub-health, which comprises the extract of stem cells as set forth in claim 1, wherein the extract of Codonopsis pilosula is obtained by the steps of: extracting radix Codonopsis in water at 90-100 deg.C under reflux to obtain extractive solution, extracting twice, mixing the extractive solutions, concentrating, centrifuging to obtain precipitate, and drying to obtain radix Codonopsis extract.

6. The agent for enhancing metabolism and immunity and improving sub-health comprising extract of stem cells according to claim 1, wherein the extract of Cordyceps sinensis is obtained by the steps of: placing Cordyceps in water, reflux-extracting at 90-100 deg.C to obtain extractive solution, adding anhydrous ethanol into the extractive solution, standing for 24-48 hr, centrifuging to obtain precipitate, and drying the precipitate to obtain Cordyceps extract.

7. The agent for enhancing metabolism and immunity and improving sub-health as claimed in claim 1, wherein the extract solution of safflower is obtained by the steps of: pulverizing Carthami flos, extracting with 70-80 v/v% ethanol water solution at 30-40 deg.C to obtain ethanol extractive solution, concentrating the ethanol extractive solution, passing through macroporous resin, eluting with 80-90 v/v% ethanol water solution, and concentrating to obtain Carthami flos extractive solution.

8. The preparation of claim 1, wherein the fresh extract of Dendrobium nobile is obtained by the following steps: crushing fresh dendrobium, placing the crushed fresh dendrobium into water, extracting the crushed fresh dendrobium in the water at the temperature of 90-100 ℃ to obtain water extract, concentrating the water extract, adding 95 v/v% ethanol water solution to dissolve the water extract, passing through an HLB (hydrophile-lipophile balance) column, eluting by using ethanol, and concentrating to obtain fresh dendrobium extract.

9. The preparation as claimed in claim 1, wherein the preparation is prepared by sterilizing with 0.22 μm sterile filter membrane, ultrafiltering to remove color, and aseptically packaging.

Technical Field

The invention relates to a preparation containing stem cell extract for enhancing metabolism and immunity and improving sub-health, belonging to the technical field of health-care food.

Background

Sub-health refers to a state of the human body between healthy and diseased. The patients in sub-health state can not reach the health standard, and the symptoms of metabolic activity reduction, immunity reduction, function and adaptability decline in a certain period of time are manifested as insomnia, dysphoria, hypodynamia, amnesia and the like, but do not meet the clinical or sub-clinical diagnosis standard of diseases related to modern medicine.

With the pace of life increasing, sub-healthy patients are increasing. The main causes of sub-health are: unreasonable diet, lack of exercise, irregular work and rest, insufficient sleep, mental stress, great psychological stress, long-term bad mood and the like.

For the treatment of sub-health patients, on one hand, the health status can be conditioned by balanced diet, regular life, proper exercise and mental relaxation, and on the other hand, the patients can be treated by other auxiliary conditioning liquids.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides a preparation containing stem cell extract for enhancing metabolism and immunity and improving sub-health.

The invention is realized by the following technical scheme:

a preparation containing stem cell extract for improving metabolism and immunity and improving sub-health, 100mL of immunopotentiator comprises the following raw materials: 10-250mg of dry cell extract freeze-dried powder, 50-650mg of beta nicotinamide mononucleotide, 1010-120mg of coenzyme Q, 1-8g of astragalus polysaccharide, 1-8g of lucid ganoderma extract, 210-240mg of ginsenoside A, 1-5g of codonopsis pilosula extract, 1-3g of cordyceps sinensis extract, 1-20mg of melatonin, 1-5g of safflower extract, 1-5g of dendrobe extract and 1-5g of epigallocatechin gallate, and the balance of deionized water.

The preparation containing the stem cell extracting solution for enhancing metabolism and immunity and improving sub-health is characterized in that the stem cell extracting solution refers to the extracting solution of the aequorea victoria stem cells.

The preparation for enhancing metabolism and immunity and improving sub-health containing the stem cell extracting solution is obtained by the following steps:

(1) cleanly breeding live hydranth of young junglenian jellyfish for 24-48 hours, repeatedly washing with sterile normal saline, sterilizing with 70% alcohol, peeling and removing tentacles under the sterile condition of 4 ℃, crushing and filtering, adding a cell separation digestive juice and a buffer culture solution to prepare a single cell suspension, centrifugally washing with normal saline for 3 times, adding a proper amount of culture medium, incubating with a dry cell marker antibody with a selective marker in a 37 ℃ carbon dioxide incubator for 45 minutes, and carrying out ice bath at 4 ℃ overnight;

(2) separating the stem cells of the cell suspension with the stem cell markers by using a flow cytometer, collecting the stem cells, centrifugally washing the stem cells for three times under the aseptic condition of 4 ℃, removing markers, and adding a buffer solution to prepare a cell suspension;

(3) adding the cell suspension into precooled buffer solution, ultrasonically breaking cells under aseptic condition, centrifuging at 4 ℃, collecting supernatant, measuring protein concentration, and preparing the suspension containing 1-2 × 10 proteins per ml⁸Stem cell extracts of individual cells;

(4) mixing the obtained stem cell extract with albumin, physiological saline mixture and adjuvants.

The preparation containing the stem cell extracting solution for enhancing metabolism and immunity and improving sub-health is prepared by the following steps: extracting Ganoderma in water at 90-100 deg.C to obtain extractive solution, concentrating the extractive solution, adding anhydrous ethanol, standing for 24-48 hr, and freeze drying to obtain Ganoderma extract.

The preparation containing the stem cell extracting solution for enhancing metabolism and immunity and improving sub-health is prepared by the following steps: extracting radix Codonopsis in water at 90-100 deg.C under reflux to obtain extractive solution, extracting twice, mixing the extractive solutions, concentrating, centrifuging to obtain precipitate, and drying to obtain radix Codonopsis extract.

The preparation containing the stem cell extracting solution for enhancing metabolism and immunity and improving sub-health is prepared by the following steps: placing Cordyceps in water, reflux-extracting at 90-100 deg.C to obtain extractive solution, adding anhydrous ethanol into the extractive solution, standing for 24-48 hr, centrifuging to obtain precipitate, and drying the precipitate to obtain Cordyceps extract.

The preparation for enhancing metabolism and immunity and improving sub-health containing the stem cell extracting solution is prepared by the following steps: pulverizing Carthami flos, extracting with 70-80 v/v% ethanol water solution at 30-40 deg.C to obtain ethanol extractive solution, concentrating the ethanol extractive solution, passing through macroporous resin, eluting with 80-90 v/v% ethanol water solution, and concentrating to obtain Carthami flos extractive solution.

The preparation for enhancing metabolism and immunity and improving sub-health containing the stem cell extracting solution is prepared from the fresh dendrobium extracting solution by the following steps: crushing fresh dendrobium, placing the crushed fresh dendrobium into water, extracting the crushed fresh dendrobium in the water at the temperature of 90-100 ℃ to obtain water extract, concentrating the water extract, adding 95 v/v% ethanol water solution to dissolve the water extract, passing through an HLB (hydrophile-lipophile balance) column, eluting by using ethanol, and concentrating to obtain fresh dendrobium extract.

The preparation for enhancing metabolism and immunity and improving sub-health containing stem cell extract is prepared by sterilizing with 0.22 μm sterile filter membrane, ultrafiltering, decolorizing, and packaging.

A stem cell is a cell that has the ability to regenerate and self-renew, i.e., has the ability to proliferate, self-repair, mass-produce, and differentiate into progeny. Has important significance for repairing human body necrosis or pathological tissue and replacing aged and apoptotic cells. However, the culture technology of the human stem cells is complex, the cost is high, dozens of ten thousand yuan can be easily treated once, the stem cells of animals and plants, particularly the beauveria aequorea stem cells can be cultured in seawater, the cost is low, a large amount of culture can be realized, the use is wide, and the benefits of the stem cells are really brought to common people.

Nicotinamide Mononucleotide (NMN) is an important substance in the production of energy in human cells, and is involved in the synthesis of intracellular NAD (nicotinamide adenine dinucleotide, an important coenzyme for cellular energy conversion). Nicotinamide mononucleotide decreases significantly with age in vivo. The research proves that the nicotinamide mononucleotide can increase NAD in various organs in a laboratory mouse, improve metabolism and increase energy supply, thereby inhibiting the aging of neurovascular and systemic tissues and organs, improving the symptoms of diabetes and the like. Especially, the expression of age-related aging genes is prevented in a tissue-specific manner, so that the oxidative metabolism of skeletal muscle mitochondria can be enhanced, and the anti-aging effect is achieved. Recent research shows that the NMN in vivo can be regulated to achieve better treatment and repair effects on cardiovascular and cerebrovascular diseases, neurodegenerative diseases, aging degenerative diseases and the like. The coenzyme Q10 has effects of improving metabolism, resisting oxidation, scavenging free radicals, protecting cells from damage, and protecting cell health. The aging of the skin and the increase of wrinkles are also related to the Q10 content, and the lower the content is, the more the skin is easily aged and the wrinkles on the face are increased. The coenzyme Q10 can increase the concentration of hyaluronic acid in skin, increase the water content of skin, and has good effects of improving dark skin color, reducing wrinkles, keeping skin smooth, elastic and moist, thereby enhancing the activity of keratinocytes, reducing apoptosis and enabling people to be younger.

Melatonin (MT) is one of the hormones secreted by the pineal gland. MT has strong neuroendocrine immunoregulation activity and free radical scavenging and antioxidant capacity. The basic functions of melatonin are to participate in the antioxidant system, prevent cells from oxidative damage, and regulate the activities of various cytokines. Has the functions of whitening, removing spots, regulating the cardiovascular and cerebrovascular functions and preventing the occurrence of tumors. Besides, the health-care pillow can also improve the immunity, improve the sleep quality and recover the sub-health.

Ginsenoside a 2: has effects in invigorating qi, supporting yang, improving cell activity, promoting stem cell proliferation, improving metabolism, enhancing immunity, restoring sub-health, and resisting aging.

The astragalus polysaccharide has the strong effects of tonifying qi, supporting yang, improving immunity, resisting virus and tumors, promoting stem cell proliferation, improving viscera functions and recovering sub-health.

Safflower is the blood-activating herb. The Carthami flos extract has effects of promoting blood circulation, removing blood stasis, resisting inflammation, resisting oxidation, improving skin blood circulation, promoting skin metabolism, and scavenging free radicals.

Ganoderma lucidum extract is known for improving immunity. Has antiviral, antitumor, viscera function balancing, metabolism promoting, and sub-health promoting effects.

The radix Codonopsis extract has effects of invigorating qi, promoting blood circulation, regulating metabolism activity, recovering tissue and organ functions, enhancing immunity, and improving sub-health state.

The cordyceps sinensis extract is mainly used for tonifying the kidney, supporting yang and benefiting qi, and has the effects of improving metabolism, enhancing immunity, recovering functions of tissues and organs, growing hair and improving sub-health.

Herba Dendrobii has antibacterial, antiinflammatory, immunity enhancing, skin hair follicle injury repairing and hair growth promoting effects. Has effects in regulating balance of yin and yang, and recovering sub-health.

Epigallocatechin gallate is the main component of tea polyphenol, has strong effects of resisting oxidation, removing free radicals, removing speckle and whitening skin, and has the functions of reducing blood lipid, promoting metabolism, protecting cardiovascular and cerebrovascular, improving immunity and recovering sub-health state.

The invention achieves the following beneficial effects:

the preparation for enhancing metabolism and immunity and improving sub-health of the invention takes stem cell extract freeze-dried powder and beta nicotinamide mononucleotide as main raw materials, and is supplemented with coenzyme Q10, melatonin, a safflower extract, a dendrobium extract, epigallocatechin gallate, astragalus polysaccharide, a ganoderma lucidum extract, ginsenoside A2, a codonopsis pilosula extract and a cordyceps sinensis extract. Can promote reprogramming and retrodifferentiation of tissue cells, promote proliferation of tissue stem cells and bone marrow, enhance immunity, promote metabolism of tissue and organ, improve functions, balance neuroendocrine regulation, coordinate visceral functions, prevent aging, relieve fatigue, improve sleep, and improve sub-health state.

The stem cell extract has various growth factors, gene regulating factors, etc. capable of promoting tissue cell reprogramming, retrodifferentiation, stem cell proliferation, epidermal cell growth, hair follicle cell growth, deep dermal collagen expression, capillary proliferation, tissue organ cell and immune cell proliferation, systemic cell metabolism activity enhancement, anti-aging, anti-apoptosis, etc. Nicotinamide mononucleotide has the functions of promoting metabolism, generating energy source matter (ATP) and promoting tissue activity.

Detailed Description

The invention is further described below. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.

Example 1

A preparation containing stem cell extract for improving metabolism and immunity and improving sub-health comprises stem cell extract lyophilized powder 10mg, beta-Nicotinamide Mononucleotide (NMN)50mg, coenzyme Q1010mg, Astragalus polysaccharides 1g, Ganoderma extract 1g, ginsenoside A210mg, radix Codonopsis extract 1g, Cordyceps extract 1g, melatonin 1mg, Carthami flos extract 1g, herba Dendrobii extract 1g and epigallocatechin gallate 1g, and deionized water in balance.

The stem cell extracting solution refers to an extracting solution of the aequorea victoria stem cells.

The extract of the aequorea victoria stem cells is obtained by the following steps:

(1) cleanly breeding the live hydranth for 24-48 hours, repeatedly washing with sterile normal saline, sterilizing with 70% alcohol, peeling and removing the skin under the sterile condition of 4 ℃, crushing and filtering, adding a cell separation digestive juice and a buffer culture solution to prepare a single cell suspension, centrifuging the normal saline to wash for 3 times, adding a proper amount of culture medium, incubating with a stem cell marker antibody with a selective marker in a carbon dioxide incubator at 37 ℃ for 45 minutes, and carrying out ice bath at 4 ℃ overnight;

(2) separating the stem cells of the cell suspension with the stem cell markers by using a flow cytometer, collecting the stem cells, centrifugally washing the stem cells for three times under the aseptic condition of 4 ℃, removing markers, and adding a buffer solution to prepare a cell suspension;

(3) adding the cell suspension into precooled buffer solution, ultrasonically breaking cells under aseptic condition, centrifuging at 4 ℃, collecting supernatant, measuring protein concentration, and preparing the suspension containing 1-2 × 10 proteins per ml⁸Stem cell extracts of individual cells;

(4) mixing the obtained stem cell extract with albumin, physiological saline mixture and adjuvants.

The ganoderma lucidum extract is obtained by the following steps: extracting Ganoderma in water at 95 deg.C to obtain extractive solution, concentrating the extractive solution, adding anhydrous ethanol, standing for 36 hr, and freeze drying to obtain Ganoderma extract.

The codonopsis pilosula extract is obtained by the following steps: extracting radix Codonopsis in water at 95 deg.C under reflux to obtain extractive solution, extracting twice, mixing the extractive solutions, concentrating, centrifuging to obtain precipitate, and drying to obtain radix Codonopsis extract.

The cordyceps sinensis extract is obtained by the following steps: placing Cordyceps in water, reflux-extracting at 95 deg.C to obtain extractive solution, adding anhydrous ethanol into the extractive solution, standing for 36 hr, centrifuging to obtain precipitate, and drying the precipitate to obtain Cordyceps extract.

The safflower extract is obtained by the following steps: pulverizing Carthami flos, extracting with 75 v/v% ethanol water solution at 35 deg.C to obtain ethanol extractive solution, concentrating the ethanol extractive solution, passing through macroporous resin, eluting with 85 v/v% ethanol water solution, and concentrating to obtain Carthami flos extractive solution.

The fresh dendrobium extract is obtained by the following steps: crushing fresh dendrobium, placing the crushed fresh dendrobium into water, extracting the crushed fresh dendrobium in the water at the temperature of 95 ℃ to obtain water extract, concentrating the water extract, adding 95 v/v% ethanol water solution to dissolve the water extract, passing through an HLB (hydrophile-lipophile balance) column, eluting by using ethanol, and concentrating to obtain fresh dendrobium extract.

A preparation containing stem cell extract for improving sub-health is prepared by sterilizing with 0.22 μm sterile filter membrane, ultrafiltering to remove color, and packaging under sterile condition.

Example 2

A preparation containing stem cell extract for improving metabolism and immunity and improving sub-health comprises stem cell extract lyophilized powder 125mg, beta-Nicotinamide Mononucleotide (NMN)325mg, coenzyme Q1060mg, Astragalus polysaccharides 4g, Ganoderma extract 4g, ginsenoside A2120mg, radix Codonopsis extract 2.5g, Cordyceps extract 2g, melatonin 10mg, Carthami flos extract 2.5g, herba Dendrobii extract 2.5g and epigallocatechin gallate 2.5g, and deionized water in balance.

The stem cell extracting solution refers to an extracting solution of the aequorea victoria stem cells.

The extract of the aequorea victoria stem cells is obtained by the following steps:

(1) cleanly breeding the live hydranth for 24-48 hours, repeatedly washing with sterile normal saline, sterilizing with 70% alcohol, peeling and removing the skin under the sterile condition of 4 ℃, crushing and filtering, adding a cell separation digestive juice and a buffer culture solution to prepare a single cell suspension, centrifuging the normal saline to wash for 3 times, adding a proper amount of culture medium, incubating with a stem cell marker antibody with a selective marker in a carbon dioxide incubator at 37 ℃ for 45 minutes, and carrying out ice bath at 4 ℃ overnight;

(2) separating the stem cells of the cell suspension with the stem cell markers by using a flow cytometer, collecting the stem cells, centrifugally washing the stem cells for three times under the aseptic condition of 4 ℃, removing markers, and adding a buffer solution to prepare a cell suspension;

(3) adding the cell suspension into precooled buffer solution, ultrasonically breaking cells under aseptic condition, centrifuging at 4 ℃, collecting supernatant, measuring protein concentration, and preparing the cell suspension containing protein per ml1-2×10⁸Stem cell extracts of individual cells;

(4) mixing the obtained stem cell extract with albumin, physiological saline mixture and adjuvants.

The ganoderma lucidum extract is obtained by the following steps: extracting Ganoderma in water at 95 deg.C to obtain extractive solution, concentrating the extractive solution, adding anhydrous ethanol, standing for 36 hr, and freeze drying to obtain Ganoderma extract.

The codonopsis pilosula extract is obtained by the following steps: extracting radix Codonopsis in water at 95 deg.C under reflux to obtain extractive solution, extracting twice, mixing the extractive solutions, concentrating, centrifuging to obtain precipitate, and drying to obtain radix Codonopsis extract.

The cordyceps sinensis extract is obtained by the following steps: placing Cordyceps in water, reflux-extracting at 95 deg.C to obtain extractive solution, adding anhydrous ethanol into the extractive solution, standing for 36 hr, centrifuging to obtain precipitate, and drying the precipitate to obtain Cordyceps extract.

The safflower extract is obtained by the following steps: pulverizing Carthami flos, extracting with 75 v/v% ethanol water solution at 35 deg.C to obtain ethanol extractive solution, concentrating the ethanol extractive solution, passing through macroporous resin, eluting with 85 v/v% ethanol water solution, and concentrating to obtain Carthami flos extractive solution.

The fresh dendrobium extract is obtained by the following steps: crushing fresh dendrobium, placing the crushed fresh dendrobium into water, extracting the crushed fresh dendrobium in the water at the temperature of 95 ℃ to obtain water extract, concentrating the water extract, adding 95 v/v% ethanol water solution to dissolve the water extract, passing through an HLB (hydrophile-lipophile balance) column, eluting by using ethanol, and concentrating to obtain fresh dendrobium extract. A preparation containing stem cell extract for improving sub-health is prepared by sterilizing with 0.22 μm sterile filter membrane, ultrafiltering to remove color, and packaging under sterile condition.

Example 3

A preparation containing stem cell extract for improving metabolism and immunity and improving sub-health comprises stem cell extract lyophilized powder 250mg, beta nicotinamide mononucleotide 650mg, coenzyme Q10120mg, astragalus polysaccharides 8g, Ganoderma extract 8g, ginsenoside A2240mg, radix Codonopsis extract 5g, Cordyceps extract 3g, melatonin 20mg, Carthami flos extract 5g, herba Dendrobii extract 5g and epigallocatechin gallate 5g, and deionized water in balance.

The stem cell extracting solution refers to an extracting solution of the aequorea victoria stem cells.

A stem cell is a cell that has the ability to regenerate and self-renew, i.e., has the ability to proliferate, self-repair, mass-produce, and differentiate into progeny. Has important significance for repairing human body necrosis or pathological tissue and replacing aged and apoptotic cells. However, the culture technology of the human stem cells is complex, the cost is high, dozens of ten thousand yuan can be easily treated once, the stem cells of animals and plants, particularly the beauveria aequorea stem cells can be cultured in seawater, the cost is low, a large amount of culture can be realized, the use is wide, and the benefits of the stem cells are really brought to common people.

The stem cell of Cypermus Turriopsis nudus used in this example was extracted from the hydroid (Hydra) which is a life form of the young (see the applicant's patent: CN105920044B, a hydroid stem cell preparation and its preparation method and application).

The body of the Hydrangea coelenterate mainly consists of stem cells. Research shows that the acaleph inherently has the capacity of 'rejuvenation', and is the only immortal organism in the world nowadays. The 'rejuvenation' of the acorn jellyfish is realized by a cell transdifferentiation process, namely, the conversion from old to young types. So, the life of the acaleph is not terminated. The jellyfishes in the lighthouse return to the beginning of life before death, and the growth and development of the jellyfishes are deduced again.

The jellyfish of lighthouse is immortal because it has a regeneration gene. If the gene capable of making the senescent cells retrodifferentiate into stem cells and the cell factors for regulating the differentiation are absorbed from the skin hair follicles and enter subcutaneous tissue cells, the retrodifferentiation of human cell genes can be regulated, human cells are rejuvenated into stem cells, and the skin can be rejuvenated.

The aequorea victoria is a collagen-rich organism. The value of the collagen of the aequorea victoria is more difficult to be compared with the value of the collagen of the aequorea victoria. Let us listen to the scientists' findings. The world's famous professor Piranio, a biologist at the University of Salento in Lecci, Italy, a marine biologist, has proven useful for us (from pharmaceuticals to nutraceuticals and even healthy beauty available). In the case of collagen, it is the major structural protein of human connective tissue, and can also be used as a scaffold for bone grafting and plastic surgery (medical and cosmetic) to implant into the human body. Not all types of animal collagen are compatible with all types of human cells. This is because jellyfish collagen is primitive and compatible with a variety of human cell types (jellies' collagen is a given and compatible with a wide variety of human cell types).

He said that if you break down collagen into small pieces, it will act as an antioxidant. Because it helps to absorb a chemical substance called free radicals, which can destroy cells (e.g., a molecular that is a chemical reactant in a cell culture of cells).

He also isolated a compound from the jellyfish that appeared to have anticancer activity. He is now investigating whether this compound is effective against a range of different cancers (he is not working to fine out of the compound is effective against a range of different cancers).

(reference: by Caled Davies, From Horizon Magazine, Horizon: The EU Research & Innovation Magazine).

The ganoderma lucidum extract is obtained by the following steps: extracting Ganoderma in water at 95 deg.C to obtain extractive solution, concentrating the extractive solution, adding anhydrous ethanol, standing for 36 hr, and freeze drying to obtain Ganoderma extract.

The codonopsis pilosula extract is obtained by the following steps: extracting radix Codonopsis in water at 95 deg.C under reflux to obtain extractive solution, extracting twice, mixing the extractive solutions, concentrating, centrifuging to obtain precipitate, and drying to obtain radix Codonopsis extract.

The cordyceps sinensis extract is obtained by the following steps: placing Cordyceps in water, reflux-extracting at 95 deg.C to obtain extractive solution, adding anhydrous ethanol into the extractive solution, standing for 36 hr, centrifuging to obtain precipitate, and drying the precipitate to obtain Cordyceps extract.

The safflower extract is obtained by the following steps: pulverizing Carthami flos, extracting with 75 v/v% ethanol water solution at 35 deg.C to obtain ethanol extractive solution, concentrating the ethanol extractive solution, passing through macroporous resin, eluting with 85 v/v% ethanol water solution, and concentrating to obtain Carthami flos extractive solution.

The fresh dendrobium extract is obtained by the following steps: crushing fresh dendrobium, placing the crushed fresh dendrobium into water, extracting the crushed fresh dendrobium in the water at the temperature of 95 ℃ to obtain water extract, concentrating the water extract, adding 95 v/v% ethanol water solution to dissolve the water extract, passing through an HLB (hydrophile-lipophile balance) column, eluting by using ethanol, and concentrating to obtain fresh dendrobium extract. A preparation containing stem cell extract for improving sub-health is prepared by sterilizing with 0.22 μm sterile filter membrane, ultrafiltering to remove color, and packaging under sterile condition.

Pharmacodynamic experiment

First, mouse experiment

The experiment is started, the preposed mouse is put in a swimming box for adaptive swimming training for 4d and 5min/d, and the water temperature is 24 +/-2 ℃. And (3) fasting and weighing 1d before the experiment, carrying out exhaustive swimming on the mice with the negative 7% of physical quantity, recording the swimming time, and rejecting the mice with the larger difference between the exhaustive swimming time and the physical quantity. And finally, 100 Kunming mice are screened out for experiment, 50 mice are taken for fatigue degree determination, and the rest 50 mice are used for blood urea nitrogen and lactic acid determination. The experiments were divided into 8 groups: the normal control group, the sub-health fatigue model group, the experimental group 1, the experimental group 2 and the experimental group 3 are 10 in each group.

Except the normal control group, the other groups adopt the method that the mice are forced to stand in water for 8 hours every day for 9 days continuously to prepare the fatigue sub-health model. The specific method comprises the following steps: the mice of each group were placed in a mouse feeding box with a water depth of 0.8cm for molding at 9:00 am every day, and were taken out from the water box at 17:00 pm every day for normal feeding.

The normal control group and the model group were normally fed without drug intervention. Experiment group 1, experiment group 2 and experiment group 3 respectively adopt the preparation of example 1, example 2 and example 3, and the preparation is intragastrically administered 1 time per day, and the intragastrically administered dose is 1 g/Kg. The mice exhaustion swimming time was measured at 9 d.

Observing the index mouse exhaustion swimming time; serum ALT, AST, BUN. Exhaustive swimming criteria: the tip of the mouse's nose was submerged for 10 consecutive seconds.

Statistical data analysis is carried out by using SPSS13.0 software, results are expressed by mean +/-standard deviation, and results are subjected to significance analysis by using independent sample t test, wherein the significance difference P is less than 0.05. The results are shown in Table 1.

Table 1 groups of mice had depleted swimming time, lactic acid and urea nitrogen levels.

	Exhaustion swimming time (min)	Lactic acid (mmol/L)	Urea nitrogen (mmol/L)
				Normal control group	1.12±0.31	9.51±1.12	8.56±0.99
Model set	0.41±0.35	9.87±1.34	10.56±1.03
				Experimental group 1	0.78±0.29	9.54±0.98	9.94±0.89
Experimental group 2	0.84±0.31	8.02±0.86	8.03±0.93
				Experimental group 3	0.96±0.29	7.36±0.92	7.18±0.91

From the results in Table 1, it is understood that the mice in the experimental groups 1 to 3 had significantly longer exhaustion swimming times than the model group, and the preparations in examples 1 to 3 were able to improve the exhaustion swimming time of the mice and the state of fatigue-type sub-health of the mice.

Compared with a normal control group, the blood lactic acid and urea nitrogen level of the model group mice has no obvious change (P is more than 0.05), and the modeling method is prompted to have no obvious influence on the blood lactic acid and urea nitrogen level of the mice. The mice of experimental groups 1-3 had significantly reduced blood urea nitrogen and lactic acid levels after swimming compared to the model group. The preparations of examples 1 to 3 can obviously improve the fatigue symptoms of the sub-health fatigue mice and reduce the clearance of blood fatigue metabolites, and have better sub-health resistance.

Second, immunological function

Selecting 32 healthy lambs (each half of male and female) with similar body weight and fetal frequency and 3-4 months of age, randomly dividing the lambs into 4 groups according to the body weight and the sex, setting a control group and a test group, adopting barn feeding, freely feeding and drinking water, feeding basic ration, and performing the test after pre-feeding for 1 week.

The animals of each group were repelled by subcutaneous injection with a conventional dose of 1% abamectin before the start of the test and the sheepcotes were disinfected. The experimental lambs were housed and each lambs was fed daily with 350g concentrate supplement, the experimental groups were added to the concentrate in amounts of 5.0g/kg, 7.5g/kg, 10.0g/kg of the formulations of example 1, respectively, and to ensure uniform mixing of the extract and concentrate, the latter were premixed and then mixed into concentrate and fed twice a day. The feed is respectively fed at the ratio of 8:30 and 16:30 in equal amount every day, and the silage corn stalks are freely eaten in the morning and the peanut seedlings (which are added in small amount for many times) are freely eaten in the afternoon, and the water is freely drunk. During the test period, the same breeder raised the animals and cleaned the pen daily. During the test period, the indoor temperature is 15-25 ℃, the outdoor temperature is 25-30 ℃ and the relative humidity is 43.5-49.7%.

SPSS11.0 statistical software is used for data processing, all data are represented by x +/-s, and the statistical significance is achieved when P is less than 0.05 by adopting t test. The results are shown in Table 2

TABLE 2 lamb immune indices for each group

The results show that the low, medium and high dose groups added with the preparation of the example can obviously improve the IgA, IgG and IgM levels compared with the control group, but the high dose group is not much different from the medium dose group, and the IgA, IgG and IgM levels of the low, medium and high dose groups change along the same trend after feeding for two weeks. The result indicates that the compound preparation has obvious influence difference on the lamb humoral immunity index and can improve the lamb immunity.

Sleep experiment

The experimental subject selects 100 female and 100 male in 35-50 years with insomnia frequency of not less than 3 times a week. The practice was to administer the formulation of example 1 to a population of subjects after dinner, and to require the subjects to maintain the original eating habits, normal diet, during the test period. The administration was continued for 12 weeks and the subjects were continuously recorded for sleep improvement. The number of insomnia times per week is taken as a basic improvement standard, and the number of insomnia times per week is taken as a remarkable improvement standard and is lower than 2.

The statistical results are shown in Table 3.

TABLE 3 statistics of the user's insomnia.

Number of insomnia/week	0 to 1 time	2-4 times	More than 4 times
				Before taking medicine	0	69％	31％
4 weeks after administration	21％	61％	18％
				8 weeks after administration	36％	54％	10％
12 weeks after administration	53％	42％	5％

Therefore, the preparation can obviously improve the insomnia condition, has better continuous taking effect, and can also better improve the insomnia which is more serious (the insomnia is treated more than 4 times per week).

The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.