阅读说明：本技术 小分子活性草酶肽及其制备方法与应用 (Small molecule active grass enzyme peptide, preparation method and application thereof ) 是由 钱康南 于 2021-01-05 设计创作，主要内容包括：本发明涉及一种小分子活性草酶肽及其制备方法与应用,本发明的小分子活性草酶肽是以蛋白草、北虫草、灵芝草、首乌草、不老草、桑枝叶、鹿霍草、紫玉米和野樱莓这九种植物为主要原料,制成的草酶肽富含多肽、多糖、多酚、玉米素、花青素、虫草素、大黄素、绿原酸、γ-氨基丁酸、白藜芦醇、异黄酮、SOD、维生素、矿物质等活性物质,人体吸收利用率大于99％,安全、无毒、无副作用,毫克剂量就能发挥作用,具有更好的药食保健价值,能预防和治疗细胞变异、抗自由基、提高免疫力,抗衰、抗菌、抗病毒,预防慢性病,是溃疡性肠炎和很多疾病的首选,本发明的草酶肽对人体、生物细胞的生长发育和预防细胞变异均有效,中老年人、妇女、儿童均可使用。(The invention relates to a small molecular active grass enzyme peptide and a preparation method and application thereof, the small molecular active grass enzyme peptide takes nine plants of proteingrass, cordyceps militaris, glossy ganoderma, polygonum multiflorum, gerbera, mulberry twig and leaf, pyrola, purple corn and wild cherry as main raw materials, the prepared grass enzyme peptide is rich in active substances such as polypeptide, polysaccharide, polyphenol, zeatin, anthocyanin, cordycepin, emodin, chlorogenic acid, gamma-aminobutyric acid, resveratrol, isoflavone, SOD, vitamin, mineral substances and the like, the absorption and utilization rate of a human body is more than 99%, the grass enzyme peptide is safe, nontoxic and free from side effects, can play a role in milligram dose, has better medicine and food health care value, can prevent and treat cellular variation, free radical resistance, immunity improvement, aging resistance, bacteria resistance, virus resistance and chronic disease prevention, and is a first choice for ulcerative enteritis and many diseases The biological cell has effects of growth and development and preventing cell variation, and can be used by middle aged and elderly people, women, and children.)

1. A small molecule active oxalate peptide, wherein the small molecule active oxalate peptide is prepared from the following raw materials: proteingrass, cordyceps militaris, ganoderma lucidum, polygonum multiflorum, gerbera, mulberry twig and leaf, pyrola, purple corn and aronia cherry.

2. The small molecule active oxalate peptide of claim 1, wherein the small molecule active oxalate peptide is prepared from the following raw materials, by weight: 35 parts of proteinic grass, 10 parts of cordyceps militaris, 5 parts of glossy ganoderma, 5 parts of polygonum multiflorum, 10 parts of gerbera, 15 parts of mulberry twig and leaf, 5 parts of pyrola, 10 parts of purple corn and 5 parts of wild cherry; preferably, the raw materials also comprise Shenzhou grass, saffron, cistanche and ginseng.

3. The small molecule active oxalate peptide of claim 2, wherein the small molecule active oxalate peptide is prepared from raw materials, the raw materials comprising, by weight: 40 parts of proteinic grass, 10 parts of cordyceps militaris, 5 parts of glossy ganoderma, 5 parts of polygonum multiflorum, 20 parts of boschniakia rossica, 5 parts of mulberry twig and leaf, 5 parts of pyrola, 5 parts of purple corn and 5 parts of wild cherry.

4. The small molecule active oxalate peptide of claim 3, wherein the small molecule active oxalate peptide is prepared from raw materials, the raw materials comprising, by weight: 50 parts of proteinic grass, 10 parts of cordyceps militaris, 5 parts of glossy ganoderma, 5 parts of polygonum multiflorum, 10 parts of gerbera, 5 parts of mulberry twig and leaf, 5 parts of pyrola, 5 parts of purple corn and 5 parts of wild cherry.

5. A method for preparing the small molecule active oxalate peptide of any of claims 1-4, comprising the steps of:

(1) weighing raw materials according to a ratio, respectively crushing, and uniformly mixing to form a mixture;

(2) adding water which is 10-15 times of the weight of the mixture obtained in the step (1) into the mixture, heating the mixture to 75 ℃, preserving heat for 10min, cooling the mixture to 40-60 ℃, adjusting the pH value to 5.5-6.5, preserving heat for 10min, adding neutral papain to carry out first enzymolysis, keeping the pH value and the temperature unchanged in the first enzymolysis process, keeping the time of the first enzymolysis for 7-9 hours, and then inactivating the enzyme for 15-30 min; cooling to 40-50 ℃, adjusting the pH to 6.0-7.5, preserving the temperature for 10-15min, adding trypsin for carrying out second enzymolysis, keeping the pH and the temperature unchanged in the second enzymolysis process, keeping the time of the second enzymolysis for 7-9 h, and then inactivating the enzyme for 15-30min to obtain an zymolyte;

(3) cooling the zymolyte obtained in the step (2) to room temperature, adjusting the pH value of the zymolyte to 7.0, centrifuging, taking supernatant, performing ultrafiltration by using an ultrafiltration membrane, obtaining filtrate, performing low-temperature concentration to form concentrated solution, and performing vacuum belt drying or freeze drying on the concentrated solution to obtain the small-molecule active oxalic enzyme peptide.

6. The method for producing a small molecule active oxalate peptide of claim 5, wherein in step (1), the powder is pulverized to a particle size of 80-100 mesh.

7. The method for preparing small molecule active oxalate enzyme peptide of claim 5, wherein in step (2), the addition amount of the neutral papain is 0.5-3% of the weight of the mixture, and the enzyme activity of the neutral papain is 32000-60000U/g.

8. The method for preparing small molecule active oxalate enzyme peptide of claim 5, wherein in step (3), the addition amount of the trypsin is 0.5-3% of the weight of the mixture, and the enzyme activity of the trypsin is 40000U/g.

9. The method for preparing small molecule active oxalate peptide of claim 5, wherein in step (3), the solid-liquid separation of the enzymatic hydrolysate is performed by a centrifuge.

10. The use of the small molecule active oxalate enzyme peptide of any of claims 1-4, wherein the small molecule active oxalate enzyme peptide is used for preparing drugs, health products, cosmetics, and the addition amount of the small molecule active oxalate enzyme peptide is 1-5 g/l.

Technical Field

The invention belongs to the technical field of health care products, and particularly relates to small-molecule active grass enzyme peptide and a preparation method and application thereof.

Background

Proteins are a source of natural life. Modern science finds that peptides, as intermediate substances between amino acids and proteins, are fundamental substances of life. Experts in the international life sciences and medical sciences agree that the twenty-first century will be the peptide century. Peptides are the source for researching life source, structure and function, and are the basis for researching diseases, physiological characteristics and cell tissues. Therefore, peptides have been included in 863 programs of our government as important contents in bioengineering and genetic engineering.

Currently, the research of peptides is leading in the world medicine field and is the most central part of human genetic engineering. In recent years, with the advent of technology, enzymatic techniques such as degradation by biological enzymes and hydrolysis, and preservation of peptide activity have become a leading problem and a new field of biotechnology.

In view of this, this patent is filed.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides a small-molecule active oxalate peptide, a preparation method and application thereof, and the prepared finished product can prevent and treat cellular variation, resist free radicals, improve immunity, resist aging, resist bacteria and viruses and prevent chronic diseases.

The invention aims to provide a small-molecule active oxalate enzyme peptide.

The invention further aims to provide a preparation method of the small-molecule active oxalate enzyme peptide.

A small molecule active oxalate peptide according to a specific embodiment of the invention is prepared from starting materials including: proteingrass, cordyceps militaris, ganoderma lucidum, polygonum multiflorum, gerbera, mulberry twig and leaf, pyrola, purple corn and aronia cherry.

The small molecule active oxalate peptide is prepared from the following raw materials in percentage by weight: 35 parts of proteinic grass, 10 parts of cordyceps militaris, 5 parts of glossy ganoderma, 5 parts of polygonum multiflorum, 10 parts of gerbera, 15 parts of mulberry twig and leaf, 5 parts of pyrola, 10 parts of purple corn and 5 parts of wild cherry; preferably, the raw materials also comprise Shenzhou grass, saffron, cistanche and ginseng.

The small molecule active oxalate peptide is prepared from the following raw materials in percentage by weight: 40 parts of proteinic grass, 10 parts of cordyceps militaris, 5 parts of glossy ganoderma, 5 parts of polygonum multiflorum, 20 parts of boschniakia rossica, 5 parts of mulberry twig and leaf, 5 parts of pyrola, 5 parts of purple corn and 5 parts of wild cherry. The small molecule active oxalate peptide is prepared from the following raw materials in percentage by weight: 50 parts of proteinic grass, 20 parts of cordyceps militaris, 25 parts of glossy ganoderma, 12.5 parts of polygonum multiflorum, 30 parts of boschniakia, 25 parts of mulberry twig and leaf, 12.5 parts of pyrola, 25 parts of purple corn and 12.5 parts of wild cherry.

A method of preparing a small molecule active oxalate peptide according to a specific embodiment of the invention, the method comprising the steps of:

(1) weighing raw materials according to a ratio, respectively crushing, and uniformly mixing to form a mixture; (2) adding water which is 10-15 times of the weight of the mixture obtained in the step (1) into the mixture, heating the mixture to 75 ℃, preserving heat for 10min, cooling the mixture to 40-60 ℃, adjusting the pH value to 5.5-6.5, preserving heat for 10min, adding neutral papain to carry out first enzymolysis, keeping the pH value and the temperature unchanged in the first enzymolysis process, keeping the time of the first enzymolysis for 7-9 hours, and then inactivating the enzyme for 15-30 min; cooling to 40-50 ℃, adjusting the pH to 6.0-7.5, preserving the temperature for 10-15min, adding trypsin for carrying out second enzymolysis, keeping the pH and the temperature unchanged in the second enzymolysis process, keeping the time of the second enzymolysis for 7-9 h, and then inactivating the enzyme for 15-30min to obtain an zymolyte;

(3) cooling the zymolyte obtained in the step (2) to room temperature, adjusting the pH value of the zymolyte to 7.0, centrifuging, taking supernatant, performing ultrafiltration by using an ultrafiltration membrane, obtaining filtrate, performing low-temperature concentration to form concentrated solution, and performing vacuum belt drying or freeze drying on the concentrated solution to obtain the small-molecule active oxalic enzyme peptide.

The method for preparing small molecule active oxalate peptide according to the embodiment of the invention comprises the step (1) of pulverizing to a particle size of 80-100 mesh. Or mixing several raw materials into a group, mixing, putting together, and mixing to obtain a mixture.

According to the preparation method of the small molecule active oxalate peptide, in the step (2), the adding amount of the neutral papain is 0.5-3% of the weight of the mixture, and the enzyme activity of the neutral papain is 32000-60000U/g.

According to the preparation method of the small-molecule active oxalate peptide, in the step (2), the adding amount of the trypsin is 0.5-3% of the weight of the mixture, and the enzyme activity of the trypsin is 40000U/g.

The method for preparing small molecule active oxalate enzyme peptide according to the embodiment of the invention, wherein, in the step (3), the ultrafiltration membrane can cut off the minimum molecular weight of 0.5 multiplied by 10⁴Da, the maximum molecular weight of the ultrafiltration membrane which can be intercepted is 1.0 multiplied by 10⁴Da。

The preparation method of the small molecule active oxalate enzyme peptide according to the embodiment of the invention comprises the step (3), wherein the temperature of the low-temperature concentration is 50-70 ℃, and the temperature of the vacuum belt drying is 50-70 ℃.

The application of the small molecular active oxalate peptide comprises the step of using the small molecular active oxalate peptide to prepare medicines, health products and cosmetics, wherein the addition amount of the small molecular active oxalate peptide is 0.1-5 g/L. Or directly infusing with warm water below 80 deg.C.

The neutral papain is a pure natural biological enzyme which is extracted by modern bioengineering technology by utilizing white milk of the epidermis of immature papaya fruits. It is a sulfhydryl (-SH) containing endopeptidase, has the activity of proteinase and esterase, has extensive specificity, and has strong hydrolytic power for animal and plant protein, polypeptide, ester and amide, at the same time, it also has synthesis function, and can synthesize protein hydrolysate into protein-like product.

The trypsin is one of protease, and is a serine proteolytic enzyme extracted from pancreas of cattle, sheep, and pig. In vertebrates, it functions as a digestive enzyme. After the pancreas is synthesized as a precursor trypsinogen of trypsin, it is secreted as a component of pancreatic juice, and it is decomposed into activated trypsin by enterokinase or restriction of trypsin, and it is an endopeptidase capable of cleaving carboxyl groups in lysine and arginine residues in polypeptide chains. It not only plays the role of digestive enzyme, but also can limit the decomposition of chymotrypsinogen, procarboxypeptidase, prophalase and other enzyme precursors, and plays the role of activation. Is a protease with the highest specificity, and is an indispensable tool in determining the amino acid sequence of proteins.

Bulaocao is also called Boschniakia rossica, has the effects of tonifying kidney and strengthening yang, and relaxing bowel, and is commonly used for treating impotence due to kidney deficiency, psychroalgia of waist and knees, constipation due to intestinal dryness, cystitis and other diseases.

The wild cherry contains abundant vitamins, anthocyanins, minerals, antioxidants and some other important polyphenols which have the effect of resisting cancer cells. Such wild berries are typically grown in the marshes of the eastern North America.

The inventor finds that the small-molecular water-soluble pearl peptide is developed 33 years ago, and through continuous research for more than 30 years, innovations are carried out from single components to compound components, patents of the invention of the three-grass peptide and the eight-grass peptide are provided, clinical tests prove that the peptide is healthier than the medicine, the peptide can prevent and treat all diseases such as cell diseases and chronic diseases, the peptide has all-round effects, and the people become young and healthy.

The inventor learns and researches for a long time, innovations are made in the combination of the traditional Chinese medicine and the high-end technology of modern biomedicine (including quantum medicine), the utilization rate and the absorption rate of the small molecular peptide 'the grass enzyme peptide' are proved to reach 99 percent again, the biosafety is more relieved, the inventor insists on using less western medicines in one day (160 above and below the hypertension and 32mol/l of hyperglycemia), takes the grass enzyme peptide invented by the inventor, takes the grass enzyme peptide (not avoiding food), runs eleven provinces for researching the grass enzyme peptide project in more than 80 years, and the three high constitutions are basically recovered to be normal.

The grass enzyme peptide is prepared from monarch, minister, assistant and guide materials such as proteinic grass, cordyceps militaris, ganoderma lucidum, fleece-flower root, boschniakia rossica, mulberry branches and leaves and the like through quantum matching, and has the highest and the most complete biological activity, for example, the dry basis of the proteinic grass contains protein, amino acid polypeptide is more than 48.7%, dietary fiber is more than 26.4%, polysaccharide is 10.4%, polyphenol, anthocyanin, resveratrol, isoflavone, SOD chlorogenic acid, cordycepin, emodin, cordycepic acid, amino frozen garlic, natural vitamins, natural minerals and hundreds of natural active substances are essences of a natural medical treasury, and the peptide particles are prepared through the processes of enzymatic hydrolysis, low-temperature concentration, vacuum drying and the like through quantum matching.

Compared with the prior art, the invention has the following beneficial effects:

(1) the small molecular active grass enzyme peptide is prepared by taking nine plants as main raw materials, is rich in active substances such as polypeptide, polysaccharide, polyphenol, zeatin, anthocyanin, cordycepin, emodin, chlorogenic acid, gamma-aminobutyric acid, resveratrol, isoflavone, SOD, vitamins, mineral substances and the like, has the absorption and utilization rate of more than 99 percent by a human body, is safe, non-toxic and free from side effects, can play a role in milligram dose, has better medicine-food health care value, can prevent and treat cellular variation, resist free radicals, improve immunity, resist aging, bacteria and viruses, prevent chronic diseases, is the first choice of ulcerative enteritis and a plurality of diseases, is effective on the growth and development of human bodies and biological cells and can prevent the cellular variation, and can be used by middle-aged and old people, women and children;

(2) the small molecular active oxalic enzyme peptide can be applied to medicines, health products and cosmetics, and the addition amount is 1-5 g/L; it can also be used for feed, fertilizer, and agricultural products, and can be prepared by mixing nontoxic and safe plants and Chinese herbal medicines such as herba Epimedii, herba Ginseng, radix Et rhizoma Fagopyri Tatarici, HEIWU, folium Ginkgo, and stigma croci Sativi;

(3) the micromolecule active grass enzyme peptide is a food which is easy to absorb and has health care function and is developed by utilizing the modern enzymolysis process and the production technology; after the traditional Chinese medicine is generally used, a better effect can be seen in ten days, the body gradually recovers to be normal, the immunity is improved, viruses are reduced, and the like.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

All the starting materials in the present invention are commercially available.

Example 1

The embodiment provides a small molecule active oxalate enzyme peptide, which is prepared from the following raw materials: 35g of proteinic grass, 10g of cordyceps militaris, 5g of glossy ganoderma, 5g of polygonum multiflorum, 10g of gerbera, 15g of mulberry twig and leaf, 5g of pyrola, 10g of purple corn and 5g of aronia serrulata;

a method for preparing a small molecule active oxalate peptide, the method comprising the steps of:

(1) weighing raw materials according to a ratio, respectively crushing, and uniformly mixing to form a mixture;

(2) adding water which is 10 times of the weight of the mixture obtained in the step (1) into the mixture, heating the mixture to 75 ℃, preserving the heat for 10min, cooling the mixture to 60 ℃, adjusting the pH value to 5.5, preserving the heat for 10min, adding neutral papain to carry out first enzymolysis, keeping the pH value and the temperature unchanged in the first enzymolysis process, keeping the time of the first enzymolysis for 9 hours, and then inactivating the enzyme for 15 min; cooling to 50 ℃, adjusting the pH to 6.0, keeping the temperature for 15min, adding trypsin for carrying out second enzymolysis, keeping the pH and the temperature unchanged in the second enzymolysis process, keeping the time of the second enzymolysis for 7 hours, and then inactivating the enzyme for 30min to obtain an zymolyte;

(3) cooling the zymolyte obtained in the step (2) to room temperature, adjusting the pH value of the zymolyte to 7.0, centrifuging to obtain supernatant, performing ultrafiltration by using an ultrafiltration membrane to obtain filtrate, performing low-temperature concentration to obtain concentrated solution, and performing freeze drying on the concentrated solution to obtain the small-molecule active oxalic enzyme peptide.

Example 2

The embodiment provides a small molecule active oxalate enzyme peptide, which is prepared from the following raw materials:

40g of proteinic grass, 10g of cordyceps militaris, 5g of glossy ganoderma, 5g of polygonum multiflorum, 20g of boschniakia rossica, 5g of mulberry twig and leaf, 5g of pyrola, 5g of purple corn and 5g of prunus mume;

a method for preparing a small molecule active oxalate peptide, the method comprising the steps of:

(1) weighing raw materials according to a ratio, respectively crushing, and uniformly mixing to form a mixture;

(2) adding water which is 10 times of the weight of the mixture obtained in the step (1) into the mixture, heating the mixture to 75 ℃, preserving the heat for 10min, cooling the mixture to 40 ℃, adjusting the pH to 5.5, preserving the heat for 10min, adding neutral papain to carry out first enzymolysis, keeping the pH and the temperature unchanged in the first enzymolysis process, wherein the time of the first enzymolysis is 7 hours, and then inactivating the enzyme for 15 min; cooling to 40 ℃, adjusting the pH to 6.0-7.5, preserving the temperature for 10min, adding trypsin for carrying out second enzymolysis, keeping the pH and the temperature unchanged in the second enzymolysis process, wherein the time of the second enzymolysis is 7 hours, and then inactivating the enzyme for 15min to obtain an zymolyte;

(3) cooling the zymolyte obtained in the step (2) to room temperature, adjusting the pH value of the zymolyte to 7.0, centrifuging, taking supernatant, performing ultrafiltration by using an ultrafiltration membrane, performing low-temperature concentration on the obtained filtrate to form concentrated solution, and performing vacuum belt drying on the concentrated solution to obtain the small-molecule active oxalic enzyme peptide.

Example 3

The embodiment provides a small molecule active oxalate enzyme peptide, which is prepared from the following raw materials:

35g of proteinic grass, 10g of cordyceps militaris, 5g of glossy ganoderma, 5g of polygonum multiflorum, 10g of gerbera, 5g of mulberry twig and leaf, 5g of pyrola, 5g of purple corn and 5g of aronia serrulata; 15g of Shenzhou grass, saffron, cistanche and ginseng;

a method for preparing a small molecule active oxalate peptide, the method comprising the steps of:

(1) weighing the raw materials according to the proportion, respectively crushing the raw materials to the particle size of 90 meshes, and uniformly mixing to form a mixture;

(2) adding water 13 times the weight of the mixture obtained in the step (1) into the mixture, heating the mixture to 75 ℃, preserving heat for 10min, cooling the mixture to 50 ℃, adjusting the pH value to 6.0, preserving heat for 10min, adding neutral papain to carry out first enzymolysis, keeping the pH value and the temperature unchanged in the first enzymolysis process, carrying out enzyme deactivation for 20min after the time of the first enzymolysis is 8 hours, wherein the addition amount of the neutral papain is 2% of the weight of the mixture, and the enzyme activity of the neutral papain is 60000U/g; cooling to 40 ℃, adjusting the pH to 7.5, keeping the temperature for 10min, adding trypsin for carrying out second enzymolysis, wherein the addition amount of the trypsin is 0.5% of the weight of the mixture, the enzyme activity of the trypsin is 40000U/g, the pH and the temperature are kept unchanged in the second enzymolysis process, the time of the second enzymolysis is 9 hours, and then carrying out enzyme deactivation for 15min to obtain an zymolyte;

(3) cooling the zymolyte obtained in the step (2) to room temperature, adjusting the pH value of the zymolyte to 7.0, centrifuging to obtain supernatant, performing ultrafiltration by using an ultrafiltration membrane, wherein the molecular weight cutoff is 0.5 multiplied by 10⁴Da-1.0×10⁴Da, carrying out low-temperature concentration at 70 ℃ on the obtained filtrate to form a concentrated solution, and carrying out vacuum belt drying on the concentrated solution at 50 ℃ to obtain the small-molecule active oxalate peptide.

Example 4

The embodiment provides a small molecule active oxalate enzyme peptide, which is prepared from the following raw materials:

40g of proteinic grass, 15g of cordyceps militaris, 5g of glossy ganoderma, 5g of polygonum multiflorum, 5g of gerbera, 5g of mulberry twig and leaf, 15g of pyrola, 5g of purple corn and 5g of aronia serrulata;

a method for preparing a small molecule active oxalate peptide, the method comprising the steps of:

(1) weighing raw materials according to a ratio, respectively crushing the raw materials to the particle size of 100 meshes, and uniformly mixing to form a mixture;

(2) adding water which is 12 times of the weight of the mixture obtained in the step (1) into the mixture, heating the mixture to 75 ℃, preserving heat for 10min, cooling the mixture to 50 ℃, adjusting the pH to 6.0, preserving heat for 10min, adding neutral papain to carry out first enzymolysis, keeping the pH and the temperature unchanged in the first enzymolysis process, carrying out enzyme deactivation for 25min after the time of the first enzymolysis is 8 hours, wherein the adding amount of the neutral papain is 3 percent of the weight of the mixture, and the enzyme activity of the neutral papain is 50000U/g; cooling to 45 ℃, adjusting the pH to 7.0, keeping the temperature for 13min, adding trypsin for carrying out second enzymolysis, wherein the addition amount of the trypsin is 4% of the weight of the mixture, the enzyme activity of the trypsin is 40000U/g, the pH and the temperature are kept unchanged in the second enzymolysis process, the time of the second enzymolysis is 8 hours, and then carrying out enzyme deactivation for 25min to obtain an zymolyte;

(3) cooling the zymolyte obtained in the step (2) to room temperature, adjusting the pH value of the zymolyte to 7.0, centrifuging to obtain supernatant, performing ultrafiltration by using an ultrafiltration membrane, wherein the molecular weight cutoff is 0.5 multiplied by 10⁴Da-1.0×10⁴Da, carrying out low-temperature concentration on the obtained filtrate at 60 ℃ to form a concentrated solution, and carrying out vacuum belt drying on the concentrated solution at 55 ℃ to obtain the small-molecule active oxalate peptide.

Example 5

The embodiment provides a small molecule active oxalate enzyme peptide, which is prepared from the following raw materials:

50g of proteingrass, 20g of cordyceps militaris, 25g of glossy ganoderma, 12.5g of polygonum multiflorum, 30g of boschniakia rossica, 25g of mulberry twig and leaf, 12.5g of pyrola, 25g of purple corn and 12.5g of wild cherry;

a method for preparing a small molecule active oxalate peptide, the method comprising the steps of:

(1) weighing raw materials according to a ratio, respectively crushing the raw materials to the particle size of 100 meshes, and uniformly mixing to form a mixture;

(2) adding water which is 10 times of the weight of the mixture obtained in the step (1) into the mixture, heating the mixture to 75 ℃, preserving heat for 10min, cooling the mixture to 40 ℃, adjusting the pH to 6.0, preserving heat for 10min, adding neutral papain to carry out first enzymolysis, keeping the pH and the temperature unchanged in the first enzymolysis process, carrying out enzyme deactivation for 30min after the time of the first enzymolysis is 7 hours, wherein the adding amount of the neutral papain is 3 percent of the weight of the mixture, and the enzyme activity of the neutral papain is 32000U/g; cooling to 40 ℃, adjusting the pH to 7.5, keeping the temperature for 10min, adding trypsin for carrying out second enzymolysis, wherein the addition amount of the trypsin is 3% of the weight of the mixture, the enzyme activity of the trypsin is 40000U/g, the pH and the temperature are kept unchanged in the second enzymolysis process, the time of the second enzymolysis is 7 hours, and then inactivating the enzyme for 30min to obtain an zymolyte;

(3) cooling the zymolyte obtained in the step (2) to room temperature, adjusting the pH value of the zymolyte to 7.0, centrifuging to obtain supernatant, performing ultrafiltration by using an ultrafiltration membrane, wherein the molecular weight cutoff is 0.5 multiplied by 10⁴Da-1.0×10⁴Da, carrying out low-temperature concentration on the obtained filtrate at 50 ℃ to form a concentrated solution, and carrying out vacuum belt drying or freeze drying on the concentrated solution at 60 ℃ to obtain the small-molecule active oxalate peptide.

The obtained small molecular active oxalic enzyme peptide is used for preparing medicines, health products and cosmetics, and the addition amount of the small molecular active oxalic enzyme peptide is 1-5 g/L. Or directly infusing with warm water below 80 deg.C.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.