阅读说明：本技术 一种提高织物毛效的生物复合物及其制备方法与应用 (Biological compound for improving fabric capillary effect and preparation method and application thereof ) 是由 宋诙 付晓平 郑雯 王梦超 郑宏臣 徐健勇 于 2020-03-27 设计创作，主要内容包括：本发明公开了一种提高织物毛效的生物复合物及其应用。本发明的生物复合物包含自行筛选得到的枯草芽胞杆菌(Bacillus subtilis)SH10、枯草芽胞杆菌(Bacillus subtilis)SH12。这些芽胞杆菌发酵液单独或一起与淀粉酶、果胶酶、脂肪酶、表面活性剂混合形成的生物复合物,对纺织物进行退浆精炼后的退浆率可达>98％,不同的织物毛效提高约1～7cm,能减少染色助剂的用量,提高后续染色工艺上色效果。同时替代传统工艺中的烧碱,节约大量能耗、水耗及废水处理费用等。应用本发明的生物复合物在纺织工艺染前处理中,对退浆、除腊、去除棉籽壳等纺织工艺处理难题有比较全面的处理优势,同时替代烧碱,环境友好。(The invention discloses a biological compound for improving the fabric capillary effect and application thereof. The biological compound comprises Bacillus subtilis SH10 and Bacillus subtilis SH12 which are obtained by self-screening. The bacillus fermentation liquor is singly or together mixed with amylase, pectinase, lipase and surfactant to form a biological compound, the desizing rate of the textile after desizing and refining can reach more than 98%, the wool effect of different textiles is improved by about 1-7 cm, the consumption of dyeing auxiliaries can be reduced, and the coloring effect of a subsequent dyeing process is improved. Meanwhile, caustic soda in the traditional process is replaced, and a large amount of energy consumption, water consumption, wastewater treatment cost and the like are saved. The biological compound has comprehensive treatment advantages on the difficult problems of textile process treatment such as desizing, dewaxing, cottonseed hull removal and the like in the textile process dyeing pretreatment, and is environment-friendly by replacing caustic soda.)

1. A biological compound capable of improving the wool effect of fabrics, which is characterized in that: the compound contains at least one fermentation liquid of bacillus subtilis SH10(CGMCC No.19322) and bacillus subtilis SH12(CGMCC No. 19323).

2. The composition as claimed in claim 1, which comprises at least one or two of the Bacillus subtilis fermentation broths as claimed in claim 1 in an amount of 20 to 900 ml/L.

3. The method for preparing a biocomposite according to claims 1 to 2, wherein the preparation method comprises: step 1, preparing seed bacteria of each strain; step 2, fermenting and carrying out enlarged culture on various kinds of the daughter bacteria obtained in the step 1; and 3, mixing the supernatant obtained after the amplification culture in the step 2 with other components in the compound to obtain the biological compound. The seed bacteria comprise Bacillus subtilis SH10, Bacillus subtilis SH12, Bacillus, Escherichia coli, yeast, etc.

4. The method according to claim 3, wherein the density of the bacillus after the scale-up culture in step 2 is not less than 1X 106 cfu/mL.

5. The biocomplex according to claims 1 to 3, characterized in that it comprises the following components:

amylase 400-4000U/ml

500-5000U/ml pectinase

100-2000U/ml lipase

2-150 g/L of surfactant

The solvent is water.

6. A biocomposite as claimed in claims 1 to 3 wherein the biocomposite is used in textile pre-printing, desizing and refining processes (cold heaps and long cars).

7. The biocomplex according to claims 1, 2, 3 and 5, wherein the amylase comprises a mesophilic amylase or a thermophilic amylase.

8. The biocomplex of claims 1, 2, 3, and 5, wherein the surfactant is any one or a combination of anionic surfactant and nonionic surfactant, including but not limited to: polysiloxane, natural fatty alcohol and ethylene oxide adduct AEO, condensate JFC of ethylene oxide and higher fatty alcohol, fatty acid methyl ester and ethylene oxide condensate FMEE or sorbitol.

9. The use of claim 6, wherein the biocomposite is used at a pH of 5.0 to 9.0.

10. The use of claim 6, wherein the biocomposite is used at a temperature in the range of 20-100 ℃.

Technical Field

The invention belongs to the technical field of textiles, and relates to a biological compound for improving the wool effect of a fabric, and a preparation method and application thereof.

Background

The textile pretreatment process aims to remove impurities such as waxy substances, pectin substances, polysaccharides, organic acids, nitrogen-containing substances, ash and the like contained in pulp and textile cellulose, and obtain good water absorption and certain whiteness, so as to be beneficial to dyeing and finishing. In continuous dyeing or printing, a fabric is padded with a dye liquor (or printing paste), the dyeing (or printing) process can be completed within only a few seconds, and the capillary effect is one of important indexes for evaluating the scouring effect of a semi-finished product and whether dyeing or printing can be performed.

The traditional textile pretreatment process generally adopts caustic soda high-temperature refining to remove non-fiber components on the surface of the fabric, such as: slurry, wax and cottonseed hulls. However, excessive caustic soda consumption easily causes fiber damage, and reduces the toughness and strength of the fabric; when the amount of the caustic soda is insufficient, the removal effect of wax and cottonseed hulls is reduced, the wettability, namely capillary effect, of the fabric is influenced, the subsequent dyeing and printing quality is further influenced, and the quality of the fabric is reduced. And the alkali scouring method generates a large amount of waste water with strong alkalinity and high COD, and cannot adapt to the trend of green textile development at home and abroad.

The application of the method for treating the biological enzyme before dyeing can save the cost of water and wastewater treatment, reduce the environmental pollution and improve the quality of products. However, the effect of only using bio-enzyme to compound and treat fabrics still has some defects, and the content and components of the sizing agent on different fabrics are variable, and basically comprise starch sizing agent, PVA sizing agent and wax. The quality of desizing and wax removing effects directly influences the wool effect of the fabric, and the key for improving the wool effect of the fabric is how to improve the desizing rate and effectively remove wax.

CN106758263B discloses a method for preparing a cotton product easy to remove dirt by a biological method, wherein laccase, lipase and acetalase are compounded to achieve the effect of removing oil stains on fabrics, and the application is a dirt removal finishing agent and does not relate to desizing and dewaxing effects. CN105970633B discloses a biological compound for textile, in the method, the auxiliary combination of various enzymes is utilized to achieve the high-efficiency desizing effect, and wax components are not decomposed or removed, however, the existence of the wax is also an important factor influencing the wettability, namely the capillary effect, of the textile.

In the field, the desizing effect and the capillary effect of the fabric after the textile pretreatment are further improved, the quality of the subsequent dyeing and printing process is improved, the dosage of the subsequent auxiliary agent is reduced, and the cost is reduced. There is a need to develop a biocomposite that is more effective in enhancing the wool effect of a fabric.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a biological compound and a preparation method and application thereof, in particular to a biological compound which can effectively improve the fabric capillary effect.

The technical scheme of the invention is as follows:

the invention provides a biological compound for textile, which comprises the following components in percentage by weight:

bacillus fermentation liquor 20-900 ml/L

Amylase 400-4000U/ml

500-5000U/ml pectinase

100-1000U/ml lipase

2-150 g/L of surfactant

The solvent is water

The Bacillus subtilis SH10(CGMCC No.19322) and the Bacillus subtilis SH12(CGMCC No.19323) are obtained by screening the soil of inner Mongolia areas for the subject group and are preserved in the China general microbiological culture Collection center (the address: Beijing city rising district North Cheng Xilu No.1 institute No. 3, China academy of sciences microbial research institute) in 1 month and 9 days of 2020.

The invention provides a preparation method of a biological compound capable of improving the fabric capillary effect, which comprises the following steps: mixing the bacillus subtilis SH10 or bacillus subtilis SH12 fermentation liquor with amylase, pectinase, lipase and surfactant according to the content of the formula components, wherein the solvent is a proper amount of water, and uniformly stirring the liquid to obtain the biological compound.

In the biological compound, the preparation method of the bacillus subtilis SH10 and the bacillus subtilis SH12 comprises the following steps: step 1, seed bacteria of SH10 and SH12 are prepared; step 2, fermenting the SH10 and SH12 seed bacteria obtained in the step 1, and carrying out amplification culture; and 3, mixing the supernatant obtained after the amplification culture in the step 2 with other components in the compound to obtain the biological compound or further compounding. The density of the cultured Bacillus is ≧ 1 × 10⁶cfu/mL。

In the biological compound, the fermentation liquor is generated by fermenting at least one of screened bacillus subtilis SH10 and bacillus subtilis SH 12. The dosage of the fermentation liquor is 20-900 ml/L. For example, 20 ml/L, 100ml/L, 500ml/L, 900 ml/L. The dosage of 500ml/L, 700ml/L and 900ml/L is preferably used for the fabric with poor fur effect.

In the biological compound, the content of the amylase is 100-5000U/mL, such as 100U/mL, 500U/mL, 1000U/mL, 3000U/mL and 5000U/mL.

In the biological compound, the content of the pectinase is 100-5000U/mL, such as 100U/mL, 500U/mL, 1000U/mL, 3000U/mL, 5000U/mL.

In the biological compound, the content of the lipase is 100-2000U/mL, such as 100U/mL, 500U/mL, 1500U/mL and 2000U/mL. The dosage of 1500U/mL and 2000U/mL are preferably used for the fabric with poor fur effect.

In the biological compound, the content of the surfactant is 2-15 g/L, such as 2g/L, 5g/L, 10g/L and 15 g/L. Preferably, the surfactant is any one or a combination of at least two of polysiloxane, natural fatty alcohol and ethylene oxide adduct AEO, condensate JFC of ethylene oxide and higher fatty alcohol, condensate FMEE of fatty acid methyl ester and ethylene oxide, or sorbitol.

The biological compound has low cost of various enzymes and fermentation products, and the applied pH range is 5.0-9.0, and the temperature range is 20-100 ℃.

The invention provides application of the biological compound in textile desizing and refining pretreatment. Preferably, the biocomposite is applied in a cold-heap or long-car process.

Compared with the prior art, the invention has the following beneficial effects:

1. the amylase in the biological compound can effectively remove starch size on the fabric, so as to achieve the ideal desizing effect; the bacillus fermentation liquor and the lipase can effectively decompose wax on the fabric fiber, the capillary effect is further improved after the wax on the surface of the fabric fiber is removed, and meanwhile, the pectinase can better act on the cotton fiber after the wax is removed, so that the pectic substance is decomposed and other hydrophobic impurities on the surface of the cotton fiber are removed. Under the four actions, the treatment condition is mild, the fiber damage is small, and the capillary effect of the fabric is gradually improved, so that the high-efficiency desizing and refining effects are achieved, and the guarantee is provided for the improvement of the quality of subsequent products.

2. The invention adopts different bacterial fermentation liquor and bio-enzyme to compound and select the content, and treats different fabrics (such as different starch slurry contents, different wax contents and different cottonseed hull contents) with the bio-compounds with different formula contents.

3. The invention uses the biological compound to replace caustic soda in the traditional textile pretreatment process, thereby saving a large amount of energy consumption, water consumption, raw material alkali, waste water treatment cost and the like. Can effectively reduce the production cost, reduce the damage of fabric fibers and increase the whiteness and capillary effect of the fabric. Is a green spinning pretreatment method.

The specific embodiment is as follows:

the technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

The material and the method are as follows:

the method for evaluating the fabric wool effect comprises the following steps: according to FZ/T01071-2008 textile capillary effect test method: three cloth strips with the width of 5cm and the length of 40cm are taken for each fabric, the rising height of the dye solution in the cloth strips for 20min is measured, and the average value is the gross head value. The larger the value of the height at which the dye rises corresponds to the better hairiness value.

The method comprises the following specific steps:

the dye fills approximately half of the beaker (at least 5cm above the bottom of the cup).

The top of the swatch was placed in the center of a thermometer clamp so that the thread was at the bottom of the clamp. The clamps were adjusted until the dye solution level was level with the line at the bottom of the fabric. And starting a timer immediately after the sample cloth is in place. The height of the dye solution that wicked up from the dye solution level after 20 minutes was measured and recorded. When the fabric supply allowed, the test was repeated 3 times for each treatment in each direction (warp, weft). The mean of the gross results was calculated for samples from the same treatment.

All test results were recorded in the laboratory manual.

Process flow

The different fabric biological desizing and refining processes include two kinds of processes, one is cold-batch process and the other is long-vehicle process. The two processes comprise the following specific steps:

the cold batch process flow comprises the following steps: biological compound preparation → padding solution → cold batch → water washing → drying → determination of capillary effect

When the pH value of the system is 5.0-9.0.

Padding the biological compound solution: padding in the biological compound working solution at the temperature of 20-60 ℃, wherein the solution carrying rate is 80-100 percent.

Cold stacking: and placing the mixture in a cold reactor at the temperature of between 20 and 40 ℃ for 6 to 12 hours.

Washing with water: and washing the substrate for 2-4 times by using hot water with the temperature of 70-80 ℃.

The long-vehicle process flow comprises the following steps:

biological compound preparation → padding solution → long vehicle → water washing → drying → determination of capillary effect.

When the pH value of the system is 5.0-9.0.

Padding the biological compound solution: padding in room-temperature biological enzyme solution, and padding twice, wherein the liquid carrying rate is 80-100%.

And (3) long vehicle: placing the mixture in saturated steam at the temperature of between 60 and 100 ℃ for 30 min.

Washing with water: and washing the substrate for 2-4 times by using hot water with the temperature of 70-80 ℃.

Example 1

In the embodiment, the Bacillus subtilis SH10 is produced by a liquid submerged fermentation method, and the method and the conditions are as follows:

fermentation medium components: corn flour 2%, peptone 3%, CaCl₂ 0.07％，Na₂HPO₄ 0.8％， pH 7.0。

2000L fermentation tank fermentation enzyme production conditions: the inoculation amount is 2%, the liquid filling coefficient is 0.65, the temperature is 37 ℃, the rotating speed is 100-200 r/min, the ventilation amount is 1: 0.5-1: 2.5, and the dissolved oxygen is maintained at 20-30%. Ammonia water and hydrochloric acid are automatically added during the fermentation process, so that the pH value of the fermentation liquor is maintained at 7.0. Meanwhile, the residual sugar concentration in the fermentation liquor is maintained by adopting an intermittent fed-batch feeding mode. Finally, the fermentation was completed for 48 h.

Example 2

In the embodiment, the Bacillus subtilis SH12 is produced by a liquid submerged fermentation method, and the method and the conditions are as follows:

fermentation medium components: corn flour 2%, peptone 3%, CaCl₂ 0.07％，Na₂HPO₄0.8% and pH 7.0. 2000L fermentation tank fermentation enzyme production conditions: the inoculation amount is 2%, the liquid filling coefficient is 0.65, the temperature is 37 ℃, the rotating speed is 100-200 r/min, the ventilation rate is 1: 0.5-1: 2.5, and the dissolved oxygen is maintained at 20-30%. Automatically feeding ammonia water and hydrochloric acid during fermentation to maintain the pH value of the fermentation liquor at 7.0. Meanwhile, the residual sugar concentration in the fermentation liquor is maintained by adopting an intermittent fed-batch feeding mode. Finally, the fermentation was completed for 48 h.

Example 3

In this example, the treated fabric was a pure cotton greige 30 x 3068 x 68, and the control group was amylase, lipase and pectinase with added surfactants to prepare a biocomposite, which included the following ingredients:

amylase 2000U/ml

500U/ml pectinase

Lipase 1000U/ml

Surfactant 2g/L

The solvent is water

The process flow comprises the following steps: long turning process

The fabrics treated in the experimental group and the process flow were the same as those in the control group except that: experimental group 1: adding 100ml of bacillus subtilis SH10 fermentation liquor fermented in example 1 into each liter of the biological compound; experiment group 2: adding 100ml of bacillus subtilis SH12 fermentation liquor fermented in example 2 into each liter of biological compound; experimental group 3: 50ml of each of the Bacillus subtilis SH10 fermentation broth fermented in example 1 and the Bacillus subtilis SH12 fermentation broth fermented in example 2 was added per liter of the biological composition.

After the long car process flow treatment, the capillary effect is measured, the average result is shown in table 1, and the results show that the capillary effect of the experimental group added with the bacillus subtilis SH10 and SH12 fermentation liquid is improved compared with that of the control group.

TABLE 1

Example 4

In this example, the treated fabric was pure cotton poplin (high wax content, poor greige) JC60 x 60^s140 x 138, control group, amylase, lipase and pectinase, added with surfactant to prepare biocomposite, comprising the following components:

amylase 2000U/ml

500U/ml pectinase

Lipase 1000U/ml

Surfactant 2g/L

The solvent is water

The process flow comprises the following steps: long turning process

The fabrics treated in the experimental group and the process flow were the same as those in the control group except that: experimental group 1: adding 100ml of bacillus subtilis SH10 fermentation liquor fermented in example 1 into each liter of the biological compound; experimental group 2: adding 100ml of bacillus subtilis SH12 fermentation liquor fermented in example 2 into each liter of biological compound; experimental group 3: 50ml of each of the Bacillus subtilis SH10 fermentation broth fermented in example 1 and the Bacillus subtilis SH12 fermentation broth fermented in example 2 was added per liter of the biological composition.

After the treatment according to the long car process flow, the capillary effect was measured, and the average results are shown in table 2. The results show that: the hair effect of the experimental group added with the bacillus subtilis SH10 and SH12 fermentation liquids is improved compared with that of the control group.

TABLE 2

Example 5

In this example, the treated fabric was cotton 40 x 40110 x 70, and the control group was amylase, lipase and pectinase with added surfactants to prepare a biocomposite, which included the following ingredients:

amylase 2000U/ml

500U/ml pectinase

Lipase 1000U/ml

Surfactant 2g/L

The solvent is water

The process flow comprises the following steps: long turning process

The fabrics treated in the experimental group and the process flow were the same as those in the control group except that: experimental group 1: adding 200ml of bacillus subtilis SH10 fermentation liquor fermented in example 1 into each liter of the biological compound; experimental group 2: adding 200ml of bacillus subtilis SH12 fermentation liquor fermented in example 2 into each liter of biological compound; experimental group 3: each liter of the biological compound was added with 100ml of each of the Bacillus subtilis SH10 fermentation broth fermented in example 1 and the Bacillus subtilis SH12 fermentation broth fermented in example 2.

After the treatment according to the long car process flow, the capillary effect was measured, and the average results are shown in table 3. The results show that: the hair effect is improved more obviously by properly increasing the using amount of the bacillus subtilis SH10 and SH12 fermentation liquids.

TABLE 3

Example 6

In this example, the treated fabric was plain cotton scrim 60 x 60140 x 140, and the control group was prepared by adding surfactants to amylase, lipase and pectinase, and the biocomposite control group included the following ingredients:

amylase 2000U/ml

500U/ml pectinase

Lipase 1000U/ml

Surfactant 2g/L

The solvent is water

The process flow comprises the following steps: cold-stacking process

The fabrics treated in the experimental group and the process flow were the same as those in the control group except that: experimental group 1: adding 100ml of Bacillus subtilis SH10 fermentation liquid into each liter of the biological compound; experimental group 2: adding 100ml of bacillus subtilis SH12 fermentation liquor to each liter of biological compound; experimental group 3: 50ml of each of bacillus subtilis SH10 fermentation liquor and bacillus subtilis SH12 fermentation liquor is added into each liter of biological compound. After treatment according to the cold-batch process, the gross effects were measured and the average results are shown in table 4. The results show that: the hair effect of the experimental group added with the bacillus subtilis SH10 and SH12 fermentation liquids is improved compared with that of the control group.

TABLE 4

Example 7

In this example, the treated fabric was pure cotton twigs (high wax content, poor flannel efficacy) 80 x 60210 x 128, and the control group was prepared by adding surfactants to amylase, lipase and pectinase to prepare biocomposites, which included the following ingredients:

amylase 2000U/ml

500U/ml pectinase

Lipase 1000U/ml

Surfactant 2g/L

The solvent is water

The process flow comprises the following steps: cold-stacking process

The fabrics treated in the experimental group and the process flow were the same as those in the control group except that: experimental group 1: adding 100ml of Bacillus subtilis SH10 fermentation liquid into each liter of the biological compound; experimental group 2: adding 100ml of bacillus subtilis SH12 fermentation liquor to each liter of biological compound; experimental group 3: 50ml of each of bacillus subtilis SH10 fermentation liquor and bacillus subtilis SH12 fermentation liquor is added into each liter of biological compound. After treatment according to the cold batch process, the gross effects were measured and the average results are shown in table 5. The results show that: the hair effect of the experimental group added with the bacillus subtilis SH10 and SH12 fermentation liquids is improved compared with that of the control group.

TABLE 5