阅读说明：本技术 一种家禽用混合物及其制备方法及应用 (Mixture for poultry and preparation method and application thereof ) 是由 王若瑾 王士举 胡麟 于 2021-04-01 设计创作，主要内容包括：本发明提供一种家禽用混合物及其制备方法及应用,该家禽用混合物的特征在于,该家禽用混合物含有：植物精油、有机酸、乳化剂、助乳化剂、增稠剂和水,所述家禽用混合物的剂型为水乳剂。本发明提供的家禽用混合物,在家禽饮水中添加,可以提高生长性能、免疫力和改善肠道菌群,大大提高了家禽养殖的生产效益。(The invention provides a poultry mixture, a preparation method and an application thereof, and the poultry mixture is characterized by comprising the following components in parts by weight: the poultry mixture is in the form of an aqueous emulsion. The poultry mixture provided by the invention is added into the poultry drinking water, so that the growth performance and the immunity can be improved, the intestinal flora can be improved, and the production benefit of poultry breeding is greatly improved.)

1. A poultry mixture comprising: the poultry mixture is in the form of an aqueous emulsion.

2. The poultry mix of claim 1, wherein the plant essential oils are carvacrol and cinnamaldehyde, the organic acid is formic acid;

based on 100 percent of the total amount of the mixture for poultry, the carvacrol is 5-10 percent, the cinnamaldehyde is 10-15 percent, and the formic acid is 3-5 percent by volume percentage.

3. The poultry blend of claim 1 wherein the emulsifier is PEG40, the co-emulsifier is glycerin, and the thickener is xanthan gum;

based on 100 percent of the total amount of the mixture for poultry, the volume percentage of the emulsifier is 10-20 percent, the volume percentage of the co-emulsifier is 1-5 percent, the volume percentage of the thickener is 0.01-0.1 percent, and the volume percentage of the water is 44.9-70.9 percent.

4. A preparation method of a mixture for poultry is characterized by comprising the following steps:

dissolving organic acid in water, adding a co-emulsifier and a thickening agent, dissolving to obtain a solution A, adding the solution A into a homogenizer, adjusting the rotation speed, and stirring and mixing for 5-10 minutes;

dissolving emulsifier in plant essential oil to obtain solution B;

adding the solution A into the solution B, controlling the rotation speed of a homogenizing machine to be 6000-.

5. The method of claim 4, wherein the plant essential oils are carvacrol and cinnamaldehyde, the organic acid is formic acid;

based on the total dosage of the mixture for poultry as 100%, the dosage of the carvacrol is 5-10%, the dosage of the cinnamaldehyde is 10-15%, and the dosage of the formic acid is 3-5% by volume percentage.

6. The method of claim 4, wherein the emulsifier is PEG40, the co-emulsifier is glycerin, and the thickener is xanthan gum;

the poultry mixture comprises, by volume percent, 10-20% of emulsifier, 1-5% of co-emulsifier, 0.01-0.1% of thickener and 44.9-70.9% of water, wherein the total amount of the poultry mixture is 100%.

7. Use of a poultry mixture according to any one of claims 1-3 for improving the growth performance of poultry.

8. Use of a mixture for poultry according to any of claims 1 to 3 for improving the immunological properties of poultry.

9. Use of a mixture for poultry according to any of claims 1 to 3 for improving the intestinal flora of poultry.

Technical Field

The invention relates to a mixture for poultry and a preparation method and application thereof.

Background

In recent years, due to the enhancement of drug resistance and harmful residues of antibiotics, the requirements of people on green, safe and pollution-free products are enhanced, and the research and development of preparations capable of replacing antibiotics in agriculture at home and abroad are very important.

The plant essential oil is widely applied to animal husbandry, has an improvement effect on the production performance of livestock and poultry, and can reduce the use amount of antibiotics. Domestic and foreign researches show that compared with the existing feed antibiotics, the animal basic ration added with the plant essential oil can obviously improve the production performance of animals; the plant essential oil can improve the immunity level of the intestinal tract, protect the integrity of villi of the small intestine, improve the physiological structure of the intestinal tract and balance the flora of the intestinal tract; can promote secretion of digestive enzymes such as cholate and pancreatic digestive enzyme, and digestive mucus.

At present, plant essential oil in the market is mainly applied to solid particles added in feed and the like, and the effect stability is poor. Therefore, there is a need to develop a poultry mixture having a good antibacterial and bactericidal effect and a good effect stability, which can replace antibiotics.

Disclosure of Invention

The invention aims to provide a poultry mixture which has good bacteriostatic and bactericidal effects on conventional pathogenic bacteria, a simple preparation method and a stable effect, a preparation method thereof and application thereof in improving the growth performance, the immunity and the intestinal flora of poultry.

The present invention provides a poultry mixture comprising: the poultry mixture is in the form of an aqueous emulsion.

Preferably, the plant essential oil is carvacrol and cinnamaldehyde, and the organic acid is formic acid.

Preferably, the carvacrol is 5-10%, the cinnamaldehyde is 10-15%, and the formic acid is 3-5% by volume percentage, based on the total amount of the poultry mixture being 100%.

Preferably, the emulsifier is PEG40 and the co-emulsifier is glycerol.

Preferably, the emulsifier is 10-20% and the co-emulsifier is 1-5% by volume percentage based on 100% of the total amount of the poultry mixture.

The thickening agent is xanthan gum; the thickening agent is 0.01-0.1% and the water is 44.9-70.9% by volume percentage based on 100% of the total amount of the poultry mixture.

The invention also provides a preparation method of the mixture for poultry, which is characterized by comprising the following steps:

dissolving organic acid in water, adding a co-emulsifier and a thickening agent, dissolving to obtain a solution A, adding the solution A into a homogenizer, adjusting the rotation speed, and stirring and mixing for 5-10 minutes;

dissolving emulsifier in plant essential oil to obtain solution B;

adding the solution A into the solution B, controlling the rotation speed of a homogenizing machine to be 6000-.

Preferably, the plant essential oil is carvacrol and cinnamaldehyde, and the organic acid is formic acid.

Preferably, the amount of the carvacrol is 5-10%, the amount of the cinnamaldehyde is 10-15%, and the amount of the formic acid is 3-5% by volume percentage, based on 100% of the total amount of the mixture for poultry.

Preferably, the emulsifier is PEG40 and the co-emulsifier is glycerol.

Based on the total dosage of the poultry mixture of 100 percent, the dosage of the emulsifier is preferably 10 to 20 percent and the dosage of the co-emulsifier is 1 to 5 percent.

The thickening agent is xanthan gum; the amount of the thickening agent is 0.01-0.1% and the amount of the water is 44.9-70.9% by volume percentage based on 100% of the total amount of the mixture for poultry.

The invention also relates to the use of a poultry mixture for improving the growth performance of poultry.

The invention also relates to the use of the mixture for poultry for improving the immunological properties of poultry.

The invention also relates to the use of a mixture for poultry for improving the intestinal flora of poultry.

The plant essential oil and the organic acid are prepared into the water emulsion type poultry mixture, so that the volatilization of the effective components can be reduced, the irritation of the plant essential oil and the organic acid to intestinal tracts can be reduced, the plant essential oil and the organic acid can be uniformly distributed by adding in a drinking water mode, and the operation is convenient. The poultry mixture provided by the invention is added into the poultry drinking water, so that the growth performance and the immunity can be improved, the intestinal flora can be improved, and the production benefit of poultry breeding is greatly improved.

Detailed Description

The poultry mixture compounded by the natural plant essential oil and the organic acid has good bacteriostatic and bactericidal effects on conventional pathogenic bacteria, can replace or reduce the use of antibiotics, can enhance the immunity of animals, and simultaneously has the effects of promoting the digestion of the animals, improving the feed conversion rate and the like. Therefore, the invention preferably uses plant essential oil and organic acid as effective components to prepare the mixture for poultry.

At present, most of products are mixed with active ingredients and then directly added into feed for feeding, however, the products are large in addition amount, various in types, inconvenient to produce and transport, poor in effect stability and greatly limited in commercialized application and popularization, and the quality of the products is influenced by a plurality of factors such as raw material producing areas and storage conditions. The plant essential oil and the organic acid are prepared into the aqueous emulsion, so that the volatilization of the effective components can be reduced, the irritation of the plant essential oil and the organic acid to the intestinal tract can be reduced, the plant essential oil and the organic acid can be uniformly distributed by adding in a drinking water mode, and the operation is convenient. Therefore, the poultry mixture is preferably prepared into the emulsion in water.

Accordingly, the present invention provides a poultry mixture comprising: the poultry mixture is in the form of an aqueous emulsion.

In addition, carvacrol, cinnamaldehyde and formic acid have antibacterial and bactericidal functions. The plant essential oil is easy to contact with bacterial lipid membrane to penetrate bacterial cell membrane, and the permeability of the cell membrane is improved by changing the phase change of double phospholipid layers of the cell membrane to trigger the overflow of cell contents. Meanwhile, formic acid is easier to permeate into thalli for dissociation, the pH level of intracellular liquid is changed to cause disorder of a bacterial metabolic system, and finally the activity of bacteria is reduced, the bacteria are exhausted and die, so that the aim of sterilization is fulfilled. The carvacrol, the cinnamaldehyde and the formic acid are mutually coordinated, and the high-efficiency sterilization effect is achieved. The carvacrol and the cinnamaldehyde can enhance the digestion and absorption of the nutrient substances by animals and improve the digestibility of the feed. The carvacrol, cinnamaldehyde and formic acid compound can improve the disease resistance of an organism and promote the growth of animals by improving and maintaining the healthy physiological state of the animals. Therefore, in the present invention, the plant essential oil is preferably carvacrol and cinnamaldehyde, and the organic acid is preferably formic acid.

Experimental research shows that when the plant essential oil and the organic acid have a certain scientific proportion, the poultry mixture has better bacteriostatic and bactericidal effects on conventional pathogenic bacteria. Therefore, in the present invention, it is preferable that the carvacrol is 5 to 10%, the cinnamaldehyde is 10 to 15%, and the formic acid is 3 to 5% by volume, based on 100% by volume of the total amount of the poultry mixture. At the moment, the poultry mixture has better bacteriostatic and bactericidal effects on conventional pathogenic bacteria.

The inventor researches and discovers that when the emulsifier is PEG40, the co-emulsifier is glycerol, and the total amount of the mixture for poultry is 100%, the emulsifier is 10-20% by volume percent, and the co-emulsifier is 1-5%, the obtained aqueous emulsion has no layering phenomenon, is more uniformly mixed, has better effect, and is preferred.

The thickener may be xanthan gum; the thickener may be 0.01 to 0.1% and the water may be 44.9 to 70.9% by volume based on 100% of the total amount of the mixture for poultry.

The preparation method of the mixture for poultry is characterized by comprising the following steps:

dissolving organic acid in water, adding a co-emulsifier and a thickening agent, dissolving to obtain a solution A, adding the solution A into a homogenizer, adjusting the rotation speed, and stirring and mixing for 5-10 minutes;

dissolving emulsifier in plant essential oil to obtain solution B;

adding the solution A into the solution B, controlling the rotation speed of a homogenizer to 7000r/min, emulsifying for 10-20min, and filtering to obtain the mixture for poultry.

In the preparation process, the emulsifying process method of the homogenizer is adopted, so that the emulsion drops of the essential oil and the organic acid are smaller, the dispersion is more uniform, the stability is better, and the problems of easy volatilization, large irritation and inconvenient addition of the plant essential oil and the organic acid are effectively solved. The emulsifier prepared by the method can improve the growth performance and immunity and improve the intestinal flora when being added into the poultry drinking water, thereby greatly improving the production benefit of poultry breeding.

The invention also relates to the use of a poultry mixture for improving the growth performance of poultry.

The invention also relates to the use of the mixture for poultry for improving the immunological properties of poultry.

The invention also relates to the use of a mixture for poultry for improving the intestinal flora of poultry.

The poultry in the invention can be common poultry, such as chicken, duck and the like.

Examples

The present invention will be further described with reference to the following specific examples, but the present invention is not limited to these examples. The technical schemes of the invention are conventional schemes in the field if not particularly stated; the reagents or materials, if not specifically mentioned, are commercially available.

Example 1

Example 1 is intended to illustrate the mixture for poultry according to the invention.

In the poultry mixture of this example, the following ingredients were used in the following amounts in volume percentage, based on 100% of the total poultry mixture: 10% of carvacrol, 15% of cinnamaldehyde, 3% of formic acid, 0% of PEG 4010%, 1% of glycerol, 0.1% of xanthan gum and 60.9% of water.

The preparation method of the mixture for poultry of this example comprises the following steps:

dissolving formic acid in a proper amount of purified water, dissolving co-emulsifier glycerin and thickener xanthan gum in water to obtain solution A, adding the solution A into a homogenizer, adjusting the rotation speed, and stirring and mixing for 5 minutes; dissolving emulsifier PEG40 in plant essential oil carvacrol and cinnamaldehyde to obtain solution B; slowly adding the solution A into the solution B, controlling the rotation speed of a homogenizer to 7000r/min, emulsifying for 10min, and then filtering to obtain the water emulsion type mixture for poultry.

The addition amount of the aqueous emulsion prepared in the embodiment in chicken drinking water is 50 g/t.

Example 2

Example 2 is intended to illustrate the mixture for poultry according to the invention.

In the poultry mixture of this example, the following ingredients were used in the following amounts in volume percentage, based on 100% of the total poultry mixture: carvacrol 5%, cinnamaldehyde 10%, formic acid 3%, PEG 4015%, glycerol 5%, xanthan gum 0.05%, and water 61.95%.

The preparation method of the mixture for poultry of this example comprises the following steps:

dissolving formic acid in a proper amount of purified water, dissolving the glycerol aid and the thickening agent xanthan gum in the water together to obtain a solution A, adding the solution A into a homogenizer, adjusting the rotating speed, and stirring and mixing for 10 minutes; dissolving PEG40 in plant essential oil carvacrol and cinnamaldehyde to obtain solution B; slowly adding the solution A into the solution B, controlling the rotation speed of a homogenizer at 6000r/min, emulsifying for 20min, and filtering to obtain the water emulsion type poultry mixture.

The addition amount of the aqueous emulsion prepared in the embodiment in chicken drinking water is 100 g/t.

Example 3

Example 3 is intended to illustrate the mixture for poultry according to the invention.

In the poultry mixture of this example, the following ingredients were used in the following amounts in volume percentage, based on 100% of the total poultry mixture: 10% of carvacrol, 10% of cinnamaldehyde, 3% of formic acid, 0% of PEG 4020%, 1% of glycerol, 0.1% of xanthan gum and 55.9% of water.

The preparation method of the mixture for poultry of this example comprises the following steps:

dissolving formic acid in a proper amount of purified water, dissolving the glycerol aid and the thickening agent xanthan gum in the water together to obtain a solution A, adding the solution A into a homogenizer, adjusting the rotating speed, and stirring and mixing for 10 minutes; dissolving PEG40 in plant essential oil carvacrol and cinnamaldehyde to obtain solution B; slowly adding the solution A into the solution B, controlling the rotation speed of a homogenizer to be 8000r/min, emulsifying for 15min, and then filtering to obtain the water emulsion type poultry mixture.

The addition amount of the aqueous emulsion prepared in the embodiment in chicken drinking water is 150 g/t.

Example 4

Example 4 is intended to illustrate the mixture for poultry according to the invention.

In the poultry mixture of this example, the following ingredients were used in the following amounts in volume percentage, based on 100% of the total poultry mixture: 5% of carvacrol, 10% of cinnamaldehyde, 5% of formic acid, 0% of PEG 4010%, 1% of glycerol, 0.1% of xanthan gum and 68.9% of water.

The preparation method of the aqueous emulsion comprises the following steps:

dissolving formic acid in a proper amount of purified water, dissolving the glycerol aid and the thickening agent xanthan gum in the water together to form a solution A, adding the solution A into a homogenizer, adjusting the rotating speed, and stirring and mixing for 10 minutes; dissolving PEG40 in plant essential oils such as carvacrol and cinnamaldehyde to obtain solution B; slowly adding the solution A into the solution B, controlling the rotation speed of a homogenizer to 7000r/min, emulsifying for 15min, and filtering to obtain a water emulsion type mixture for poultry.

The addition amount of the aqueous emulsion prepared in the embodiment in chicken drinking water is 200 g/t.

Example 5

Example 5 is intended to illustrate the effect of the combination of a preferred plant essential oil and an organic acid in the poultry mixture of the invention, in comparison with example 1.

In this example, a water emulsion type mixture for poultry was obtained in the same manner as in the preparation method of example 1, except that carvacrol 25% was used instead of the combination of carvacrol 10% and cinnamaldehyde 15%.

Example 6

Example 6 is intended to illustrate the effect of the combination of a preferred plant essential oil and an organic acid in the poultry mixture of the invention, in comparison with example 1.

In this example, a water emulsion type mixture for poultry was obtained in the same manner as in the preparation method of example 1, except that cinnamaldehyde 25% was used instead of the combination of carvacrol 10% and cinnamaldehyde 15%.

Example 7

Example 7 is intended to illustrate the effect of the preferred ratio of plant essential oil to organic acid in the poultry mixture of the present invention, in comparison with example 1.

In this example, a water emulsion type mixture for poultry was obtained in the same manner as in the preparation method of example 1 except that 8% of cinnamaldehyde and 10% of formic acid were used instead of 15% of cinnamaldehyde and 3% of formic acid.

Example 8

Example 8 is intended to illustrate the effect of the selection and ratio of preferred emulsifiers and co-emulsifiers in the poultry mixture of the present invention, in comparison with example 1.

In this example, a water emulsion type mixture for poultry was obtained in the same manner as in the preparation method of example 1 except that PEG 4010.9% and glycerin 0.1% were used instead of the mixture ratio of PEG 4010% and glycerin 1%.

Comparative example 1

Comparative example 1 is used for comparison with example 1, and illustrates the effect of the mixture for poultry of the present invention, which uses a combination of plant essential oils and organic acids as effective ingredients, and is made into an aqueous emulsion.

In this comparative example, a combination of carvacrol 10%, cinnamaldehyde 18% was used instead of the combination of carvacrol 10%, cinnamaldehyde 15%, formic acid 3%, and PEG40 and glycerin were not used, and the top-up with water, to give a mixture solution for poultry of comparative example 1, without forming an aqueous emulsion.

The poultry mixtures of examples 1 to 8 and comparative example 1 were evaluated by specific experiments.

1. Stability test

Dilution stability: diluting 1ml of aqueous emulsion by 20 times of tap water, uniformly stirring, standing for 1h, and determining that no precipitate or floating oil is qualified.

Heat storage stability: the sealed sample was placed at 54 + -2 deg.C and 60% + -5% relative humidity to observe whether delamination occurred.

Cold storage stability: and (3) subpackaging the prepared aqueous emulsion into 10ml centrifuge tubes, storing for 7d in a refrigerator at 4 ℃, if oil separation, precipitation or layering occurs at low temperature, placing at room temperature or shaking properly to restore the original state, and ensuring that the low-temperature stability is qualified, otherwise, the low-temperature stability is not qualified.

Average particle size: the particle size of more than 600 particles is measured by a microscopic image photographing system, the average value is calculated, and whether the submicron emulsion level is reached or not is observed.

Stability constant: the percentage of change in optical density before and after centrifugation of the emulsion is called the stability constant, and the smaller the value of Ke, the more stable the emulsion is, and the expression is as follows:

Ke＝(Ao-A)/A×100％

wherein Ao is the absorbance of the emulsion diluent not centrifuged; a-absorbance of emulsion dilutions after centrifugation.

The poultry mixtures of examples 1 to 8 and comparative example 1 were evaluated for stability by the above-described method, and the results are shown in Table 1.

TABLE 1 stability evaluation

The results in Table 1 show that the aqueous creams in examples 1 to 8 are all relatively good in stability, and comparative example 1 does not form an aqueous cream and is poor in stability.

2. Test animals and test design

Test 1 day old chicks 500 were randomly divided into 10 groups (1 control group, 8 example groups (corresponding to examples 1-8, respectively), and 1 comparative example group (corresponding to comparative example 1)), each group containing 100 chickens (the specific grouping is shown in table 2), and the poultry mixture was added to the daily drinking water of chickens according to the following protocol, 10 chickens were added to each group, and 10 chickens were added to each group. The test period was 42 d. The remaining drugs used for disease control were identical during the trial. And (3) weighing and then slaughtering after the feeding test is finished, collecting blood from jugular veins, analyzing the growth performance and blood biochemical indexes of the broilers, randomly selecting 10 chickens from each group to slaughter (50 chickens in total), and detecting the quantity of bacteria in intestinal tracts and excrement. Collecting small intestine samples, measuring the villus height and crypt depth of the small intestine, and carrying out data statistical analysis.

The weight is measured in replicates before and after the start and end of the test and the weight data is recorded. Average daily gain is (end weight-initial weight)/test day, and average daily food intake is total food intake/test day.

And (3) taking the thawed serum, determining the contents of IgA, IgG and IgM by adopting an immune transmission turbidimetry method, and detecting the blood antioxidant index by adopting ELISA.

And (3) detecting the content of bacteria: and detecting the expression amount and the relative expression amount of escherichia coli, salmonella and lactobacillus by adopting a fluorescent quantitative PCR method.

To determine the morphology of the intestinal tissue, tissue samples were dehydrated and embedded in paraffin, then cut to 6 μm thickness and stained with hematoxylin and eosin. The tissue sections were observed using an optical microscope system (Olympus Corporation, japan). Intestinal villus height (villus top to villus-crypt junction) and crypt depth (vertical distance from intestinal villus junction to intestinal gland bottom) were measured with ImagePro plus 6.0 and villus height/crypt depth was calculated.

The test data were statistically analyzed using SPSS19.0 statistical software, and the results are shown in the table below.

Table 2 experimental grouping and treatment

TABLE 3 growth Performance index

As shown by the results in Table 3, the daily feed intake and daily weight gain of the chickens in examples 1-8 are significantly increased compared with those of the control group and comparative example 1, which shows that the chickens in examples 1-8 have the effects of improving the production performance and immunity, and the effect of example 3 is the best.

In addition, the effect of daily feed intake and daily gain was better in example 1 in which the combination of carvacrol, cinnamaldehyde and formic acid was used as an active ingredient, compared to examples 5 to 6 in which the combination of carvacrol, cinnamaldehyde and formic acid was not used as an active ingredient, which indicates that the productivity of chickens could be further improved when the combination of carvacrol, cinnamaldehyde and formic acid was used as an active ingredient.

Compared with the example 7 in which the ratio of the cinnamaldehyde to the formic acid is not in the range of 10-15% of the cinnamaldehyde and 3-5% of the formic acid which are preferred in the invention, the effect of daily feed intake and daily gain of the example 1 in which the ratio of the cinnamaldehyde to the formic acid is in the preferred range is better, which shows that the ratio of the cinnamaldehyde to the formic acid is in the preferred range, and the production performance of the chicken can be further improved.

Compared with the example 8 in which the ratio of the emulsifier to the co-emulsifier is not in the range of 10-20% and 1-5% of the preferred emulsifier in the invention, the daily feed intake and daily gain of the example 1 in which the ratio of the emulsifier to the co-emulsifier is in the preferred range have better effects, which shows that the production performance of the chicken can be further improved when the ratio of the emulsifier to the co-emulsifier is in the preferred range.

Compared with comparative example 1 in which the combination of plant essential oil and organic acid is not selected as an active ingredient and the aqueous emulsion is not prepared, the daily feed intake and the daily gain of example 1 in which the aqueous emulsion is prepared are significantly increased by selecting the combination of plant essential oil and organic acid as the active ingredient, which indicates that the productivity of chickens can be significantly improved when the aqueous emulsion is prepared by selecting the combination of plant essential oil and organic acid as the active ingredient.

TABLE 4 serum antioxidant and immune index

As shown by the results in Table 4, the total antioxidant capacity of serum, the content of glutathione peroxidase, and the contents of globulin IgA, IgM and IgG in examples 1 to 8 are all increased to some extent or significantly higher than those of the control group, which indicates that all examples 1 to 4 can play a role in improving the immunity of the organism, and the effect of example 3 is the most excellent.

In addition, compared with examples 5 to 6 in which the combination of carvacrol, cinnamaldehyde and formic acid was not used as an active ingredient, the serum total antioxidant capacity, the glutathione peroxidase content, and the globulin IgA, IgM and IgG content were all increased to some extent in example 1 in which the combination of carvacrol, cinnamaldehyde and formic acid was used as an active ingredient, which indicates that the combination of carvacrol, cinnamaldehyde and formic acid was used as an active ingredient, which had an effect of further improving the body immunity.

Compared with the example 7 in which the proportion of the cinnamaldehyde to the formic acid is not in the range of 10-15% of the cinnamaldehyde and 3-5% of the formic acid which are preferred in the invention, the serum total antioxidant capacity, the content of glutathione peroxidase, the contents of globulin IgA, IgM and IgG of the example 1 in which the proportion of the cinnamaldehyde to the formic acid is in the preferred range are all increased to a certain extent, which shows that the proportion of the cinnamaldehyde to the formic acid in the preferred range can play a role in further improving the body immunity.

Compared with the example 8 in which the proportion of the emulsifier and the co-emulsifier is not in the range of 10-20% and 1-5% of the preferred emulsifier in the invention, the serum total antioxidant capacity, the content of glutathione peroxidase, the content of globulin IgA, IgM and IgG of the example 1 in which the proportion of the emulsifier and the co-emulsifier is in the preferred range are increased to a certain extent, which shows that the proportion of the emulsifier and the co-emulsifier in the preferred range can play a role in further improving the body immunity.

Compared with the comparative example 1 in which the combination of the plant essential oil and the organic acid is not selected as the effective component and the aqueous emulsion is not prepared, the serum total antioxidant capacity, the glutathione peroxidase content, the globulin IgA, IgM and IgG content of the aqueous emulsion prepared from the combination of the plant essential oil and the organic acid in example 1 are remarkably increased, which shows that the combination of the plant essential oil and the organic acid is selected as the effective component and the aqueous emulsion prepared from the combination of the plant essential oil and the organic acid can effectively improve the body immunity.

TABLE 5 Small intestinal villus height and crypt depth

The villus height, crypt depth, and villus height/crypt depth of the small intestine are important indicators for evaluating nutrient digestion and absorption by the animal digestive tract. The larger the intestinal villus height is, the better the intestinal epithelial cell development is, the villus height influences the nutrient absorption capacity, and conversely, the shallower the crypt depth is, the higher the intestinal epithelial cell maturation rate is. A decrease in villus height/crypt depth indicates impaired absorption function and thus an increased likelihood of diarrhea.

As shown by the results in Table 5, the small intestine villus heights in examples 1-8 were all increased relative to the control group, indicating that examples 1-8 all function to improve intestinal health and function, reduce crypt depth, and alleviate atrophy of small intestine villus, with the best results being shown in example 3.

In addition, compared to examples 5 to 6 in which the combination of carvacrol, cinnamaldehyde and formic acid was not used as an active ingredient, the small intestine villus height was increased to some extent and the crypt depth was decreased to some extent in example 1 in which the combination of carvacrol, cinnamaldehyde and formic acid was used as an active ingredient, which indicates that the combination of carvacrol, cinnamaldehyde and formic acid was used as an active ingredient to further improve the intestinal health and function.

Compared with the example 7 in which the ratio of the cinnamaldehyde to the formic acid is not in the range of 10-15% of the cinnamaldehyde and 3-5% of the formic acid which are preferred in the invention, the small intestine villus height of the example 1 in which the ratio of the cinnamaldehyde to the formic acid is in the preferred range is increased to a certain extent, and the crypt depth is reduced to a certain extent, which shows that the ratio of the cinnamaldehyde to the formic acid is in the preferred range, and the intestinal health and the functions can be further improved.

Compared with the example 8 in which the ratio of the emulsifier to the co-emulsifier is not in the range of 10-20% and 1-5% of the preferred emulsifier in the invention, the small intestine villus height of the example 1 in which the ratio of the emulsifier to the co-emulsifier is in the preferred range is increased to a certain extent, and the crypt depth is reduced to a certain extent, which shows that the ratio of the emulsifier to the co-emulsifier is in the preferred range, the intestinal health and function can be further improved.

Compared with the comparative example 1 in which the combination of the plant essential oil and the organic acid is not selected as the effective component and the aqueous emulsion is not prepared, the small intestine villus height of the example 1 in which the combination of the plant essential oil and the organic acid is selected as the effective component and the aqueous emulsion is prepared is remarkably increased, and the crypt depth is remarkably reduced, which shows that the combination of the plant essential oil and the organic acid is selected as the effective component and the aqueous emulsion is prepared, so that the effects of effectively improving the intestinal health and functions can be achieved.

TABLE 6 E.coli content in intestinal tract and faeces (Log10(cfu/g))

As shown by the results in Table 6, the E.coli content in each of the intestinal tracts (cecum, colon, rectum) and feces in examples 1-8 was significantly reduced relative to the control group, indicating that each of examples 1-8 functions against E.coli, a harmful intestinal bacterium, and the effect is most excellent as in example 3.

In addition, in example 1 in which the combination of carvacrol, cinnamaldehyde and formic acid was used as an active ingredient, the content of escherichia coli in each intestinal tract (cecum, colon, rectum) and feces was decreased to some extent, as compared with examples 5 to 6 in which the combination of carvacrol, cinnamaldehyde and formic acid was not used as an active ingredient, indicating that the combination of carvacrol, cinnamaldehyde and formic acid was used as an active ingredient, and thus it was possible to further resist intestinal harmful bacteria escherichia coli.

Compared with the example 7 in which the ratio of the cinnamaldehyde to the formic acid is not in the range of 10 to 15% of the cinnamaldehyde and 3 to 5% of the formic acid, which is the preferred range of the present invention, the example 1 in which the ratio of the cinnamaldehyde to the formic acid is in the preferred range has a certain decrease in the content of escherichia coli in each intestinal tract (cecum, colon, rectum) and feces, which indicates that the ratio of the cinnamaldehyde to the formic acid is in the preferred range, and the effect of further resisting intestinal harmful bacteria escherichia coli can be achieved.

Compared with the example 8 in which the proportion of the emulsifier and the co-emulsifier is not in the range of 10-20% and 1-5% of the preferred emulsifier in the invention, the example 1 in which the proportion of the emulsifier and the co-emulsifier is in the preferred range has a certain reduction in the content of escherichia coli in each intestinal tract (cecum, colon and rectum) and feces, which shows that the proportion of the emulsifier and the co-emulsifier in the preferred range can play a role in further resisting harmful intestinal escherichia coli.

Compared with the comparative example 1 in which the combination of the plant essential oil and the organic acid is not selected as the effective component and the aqueous emulsion is not prepared, the content of escherichia coli in each intestinal tract (cecum, colon and rectum) and excrement is remarkably reduced in the example 1 in which the combination of the plant essential oil and the organic acid is selected as the effective component and the aqueous emulsion is prepared, which shows that the effect of effectively resisting intestinal harmful bacteria escherichia coli can be achieved when the combination of the plant essential oil and the organic acid is selected as the effective component and the aqueous emulsion is prepared.

TABLE 7 Salmonella content in intestinal and faecal tract (Log10(cfu/g))

As shown by the results in Table 7, the content of Salmonella in each of the intestinal tracts (cecum, colon, rectum) and feces in the test groups 1 to 8 was significantly reduced compared to the control group, indicating that all of the test groups 1 to 8 were effective against Salmonella, which is an intestinal bacterium harmful to the intestines, and the effect was the most excellent in the test group 3.

In addition, compared with examples 5 to 6 in which the combination of carvacrol, cinnamaldehyde and formic acid was not used as an active ingredient, example 1 in which the combination of carvacrol, cinnamaldehyde and formic acid was used as an active ingredient exhibited a certain decrease in the content of salmonella in each intestinal tract (cecum, colon, rectum) and feces, indicating that the combination of carvacrol, cinnamaldehyde and formic acid was used as an active ingredient, and thus exhibited an effect of further combating salmonella, a harmful bacterium in intestinal tract.

Compared with the example 7 in which the ratio of the cinnamaldehyde to the formic acid is not in the range of 10-15% of the cinnamaldehyde and 3-5% of the formic acid which are preferred in the invention, the example 1 in which the ratio of the cinnamaldehyde to the formic acid is in the preferred range has a certain reduction in the content of salmonella in each intestinal tract (cecum, colon, rectum) and feces, which shows that the ratio of the cinnamaldehyde to the formic acid in the preferred range can further resist the salmonella enterica.

Compared with the example 8 in which the proportion of the emulsifier and the co-emulsifier is not in the range of 10-20% and 1-5% of the preferred emulsifier in the invention, the content of salmonella in each intestinal tract (cecum, colon and rectum) and feces is reduced to a certain extent in the example 1 in which the proportion of the emulsifier and the co-emulsifier is in the preferred range, which shows that the proportion of the emulsifier and the co-emulsifier in the preferred range can play a role in further resisting the intestinal harmful bacteria salmonella.

Compared with the comparative example 1 in which the combination of the plant essential oil and the organic acid is not selected as the effective component and the aqueous emulsion is not prepared, the content of salmonella in each intestinal tract (cecum, colon and rectum) and feces is remarkably reduced in the example 1 in which the combination of the plant essential oil and the organic acid is selected as the effective component and the aqueous emulsion is prepared, which shows that the combination of the plant essential oil and the organic acid is selected as the effective component and the aqueous emulsion can effectively resist the salmonella which is a harmful bacterium in the intestinal tract.

TABLE 8 lactic acid bacteria content in intestinal tract and faeces (Log10(cfu/g))

As shown by the results in table 8, the content of lactic acid bacteria in each of the intestinal tracts (cecum, colon, rectum) and feces in the test groups 1 to 8 was significantly increased compared to the control group, which indicates that the test groups 1 to 8 all function to promote lactic acid bacteria that are beneficial bacteria in the intestinal tracts, and the effect of the test group 3 was the most excellent.

In addition, in example 1 in which the combination of carvacrol, cinnamaldehyde and formic acid was used as the active ingredient, the content of lactic acid bacteria in each intestinal tract (cecum, colon, rectum) and feces was increased to some extent, as compared with examples 5 to 6 in which the combination of carvacrol, cinnamaldehyde and formic acid was not used as the active ingredient, which indicates that the combination of carvacrol, cinnamaldehyde and formic acid was used as the active ingredient, and it was possible to further promote the beneficial bacteria lactic acid bacteria in the intestinal tract.

Compared with the example 7 in which the ratio of the cinnamaldehyde to the formic acid is not in the range of 10-15% and 3-5% of the cinnamaldehyde which is the preferable range in the invention, the content of the lactic acid bacteria in each intestinal tract (cecum, colon and rectum) and feces is increased to a certain extent in the example 1 in which the ratio of the cinnamaldehyde to the formic acid is in the preferable range, which shows that the ratio of the cinnamaldehyde to the formic acid is in the preferable range, and the function of further promoting the beneficial bacteria lactic acid bacteria in the intestinal tract can be achieved.

Compared with the example 8 in which the proportion of the emulsifier and the co-emulsifier is not in the range of 10-20% and 1-5% of the preferred emulsifier in the invention, the example 1 in which the proportion of the emulsifier and the co-emulsifier is in the preferred range has certain increase of the content of the lactobacillus in each intestinal tract (cecum, colon and rectum) and feces, which shows that the proportion of the emulsifier and the co-emulsifier in the preferred range can play a role in further promoting the beneficial bacteria lactobacillus in the intestinal tract.

Compared with the comparative example 1 in which the combination of the plant essential oil and the organic acid is not selected as the effective component and the aqueous emulsion is not prepared, the content of the lactobacillus in each intestinal tract (cecum, colon and rectum) and feces is remarkably increased in the example 1 in which the combination of the plant essential oil and the organic acid is selected as the effective component and the aqueous emulsion is prepared, which shows that the effect of effectively promoting the intestinal beneficial bacterium lactobacillus can be achieved when the combination of the plant essential oil and the organic acid is selected as the effective component and the aqueous emulsion is prepared.

The present invention has been described above with reference to specific examples, but the present invention is not limited to these specific examples. In addition, certain terminology used in the description and claims of the present application is not limiting, but is used for convenience only.