阅读说明：本技术 一种替抗促生长的美洲大蠊内源活性复合物添加剂 (Periplaneta americana endogenous activity compound additive for resisting and promoting growth ) 是由 李延荣 刘雪兰 左立 姜丽娟 张立伟 李成 伏春燕 张燕 董以雷 王文彬 阎佩佩 于 2021-01-28 设计创作，主要内容包括：本发明提供了一种替代抗生素的复合饲料添加剂。本发明将美洲大蠊的水提物、醇提物、酶解物、大蠊卵鞘超微粉等按照科学的比例混合在一起,在饲料中添加可增强动物机体天然免疫力,预防动物疫病的发生,促进营养物质消化吸收,促进动物生长,可以完全替代抗生素的使用。(The invention provides a compound feed additive for replacing antibiotics. The invention mixes the water extract, alcohol extract, zymolyte, superfine powder of periplaneta ovalae ootheca and the like of the periplaneta americana together according to a scientific proportion, and adds the superfine powder into the feed, which can enhance the natural immunity of animal organisms, prevent the occurrence of animal epidemic diseases, promote the digestion and absorption of nutrient substances, promote the growth of animals and completely replace antibiotics.)

1. An American cockroach endogenous activity compound additive for resisting and promoting growth is characterized by comprising the following components in parts by weight: 7-11% of periplaneta americana aqueous extract, 0.3-0.5% of periplaneta americana alcohol extract, 10-15% of periplaneta americana enzymatic hydrolysate, 15-20% of periplaneta americana ootheca superfine powder and the balance of carrier.

2. The periplaneta americana endogenous activity complex additive for resisting and promoting growth according to claim 1, which is characterized by comprising the following components in percentage by weight: 8-10% of periplaneta americana aqueous extract, 0.3-0.4% of periplaneta americana alcohol extract, 12-14% of periplaneta americana zymolyte, 16-19% of periplaneta americana ootheca superfine powder and the balance of carrier.

3. The periplaneta americana endogenous activity complex additive for resisting and promoting growth according to claim 2, which is characterized by comprising the following components in percentage by weight: the composition comprises 10% of periplaneta americana aqueous extract, 0.4% of periplaneta americana alcohol extract, 14% of periplaneta americana zymolyte, 19% of periplaneta americana ootheca superfine powder and the balance of a carrier.

4. The periplaneta americana endogenously active complex additive according to any one of claims 1-3 for resisting growth promotion, characterized in that the periplaneta americana aqueous extract is an aqueous extract obtained after extracting the periplaneta americana with distilled water.

5. The periplaneta americana endogenous activity complex additive according to any one of claims 1 to 3 for resisting and promoting growth, wherein the periplaneta americana alcohol extract is an alcohol extract obtained by extracting periplaneta americana powder with 70% ethanol.

6. The periplaneta americana endogenous activity complex additive as claimed in any one of claims 1 to 3 for resisting and promoting growth, wherein the periplaneta americana zymolyte is periplaneta americana powder firstly adopting supercritical CO₂After the grease is removed by extraction, the supernatant fluid obtained after the enzymolysis by adopting the alkaline endoprotease is frozen and dried to obtain the zymolyte.

7. The periplaneta americana endogenously active complex additive according to any one of claims 1-3, characterized in that the carrier is zeolite powder.

8. The periplaneta americana endogenous active complex additive for resisting and promoting growth according to any one of claims 1 to 3, wherein the granularity of the superfine powder of the periplaneta ovalae ootheca is 500-mesh sieve.

Technical Field

The invention belongs to the field of animal nutrition and feed science, relates to a natural compound feed additive, and particularly relates to a natural compound feed additive for replacing antibiotics.

Background

There are a number of problems associated with the long-term use of large doses of antibiotics: the animals show persistent drug resistance; antibiotic drug residues enter the human body along with the food chain, thus harming the health of the human body; the balance of animal intestinal flora is destroyed, so that the immunity of the animal is reduced; antibiotics can not be completely absorbed in animal bodies, about 60-90% of the antibiotics are discharged out of the bodies along with excrement of animals, and the environment is polluted. For human health and human safety concerns, the agricultural countryside issued a 194 th bulletin regarding feed resistance.

At present, a plurality of potential substitutes of antibiotics exist, but no single feed additive can replace the feed antibiotics. Periplaneta americana is one of cockroaches, one of the oldest insects in the world, born before dinosaurs. Only German cockroach and other few cockroach species are pests, and the rest over 5000 species are unbearable beneficial pests. Periplaneta americana is an outstanding good one of the beneficial insects, is a natural traditional Chinese medicinal material, contains various natural antibacterial ingredients, and has strong in-vitro antibacterial activity on staphylococcus aureus, Escherichia coli and pseudomonas aeruginosa; is rich in immune enhancing factors and growth promoting factors and has the function of promoting growth by immunity; in addition, it also has the functions of promoting cell proliferation, promoting the growth of new granulation tissue, accelerating the repair of damaged tissue, etc. Therefore, the periplaneta americana is a potential natural anti-growth promoting substance.

In recent years, a great deal of research on periplaneta americana has been carried out by many scholars, and particularly in the field of human medicine, a medicine developed by using the periplaneta americana as a raw material is approved and prescribed by over ten thousand clinical experts in over 4000 hospitals across the country, and has become a stock medicine for 10 ten thousand primary medical units. But no related product development and application reports are found in the field of livestock and poultry breeding. In view of the requirements of national policies and the requirements of the breeding industry, research and development work of periplaneta americana-derived series products is carried out.

Disclosure of Invention

Aiming at the problems, the invention provides a compound feed additive for replacing antibiotics. The invention mixes the water extract, alcohol extract, zymolyte, superfine powder of periplaneta ootheca of the American cockroach and the like together according to a scientific proportion, and the addition of the superfine powder can enhance the natural immunity of animal organisms, prevent the occurrence of animal epidemic diseases, promote the digestion and absorption of nutrient substances and promote the growth of animals.

The technical scheme adopted by the invention is as follows: a compound feed additive for replacing antibiotics is characterized by comprising the following components in percentage by weight: 7-11% of periplaneta americana aqueous extract, 0.3-0.5% of periplaneta americana alcohol extract, 10-15% of periplaneta americana enzymatic hydrolysate, 15-20% of periplaneta americana ootheca superfine powder and the balance of carrier.

Preferably, the composite additive comprises the following components in percentage by weight: 8-10% of periplaneta americana aqueous extract, 0.3-0.4% of periplaneta americana alcohol extract, 12-14% of periplaneta americana zymolyte, 16-19% of periplaneta americana ootheca superfine powder and the balance of carrier.

More preferably, the composite additive comprises the following components in percentage by weight: the composition comprises 10% of periplaneta americana aqueous extract, 0.4% of periplaneta americana alcohol extract, 14% of periplaneta americana zymolyte, 19% of periplaneta americana ootheca superfine powder and the balance of a carrier.

Preferably, the carrier is zeolite powder.

Preferably, the granularity of the cockroach ootheca superfine powder is 500 meshes. The particle sizes of the water extract, the alcohol extract and the zymolyte of the periplaneta americana are all sieved by a sieve of 80 meshes.

The using method comprises the following steps: the compound feed additive is directly added into feed, and the addition amount is 0.1%.

The raw materials and the functions are as follows:

the American cockroach aqueous extract comprises the following components: the main component of the water extract obtained after extracting the periplaneta americana with distilled water is functional amino acids.

The periplaneta americana alcohol extract: the periplaneta americana powder is an alcohol extract obtained by extracting with 70% ethanol. The main components are lobster carnosine, protocatechuic acid glucoside, trigonelline and other glycosides and alkaloids, and have the effects of repairing intestinal mucosa, resisting inflammatory reaction and improving intestinal mucosa immunity.

Periplaneta americana zymolyte: the periplaneta americana powder firstly adopts supercritical CO₂After the grease is removed by extraction, the supernatant fluid obtained after the enzymolysis by adopting the alkaline endoprotease is frozen and dried to obtain the zymolyte. The main component is small peptide, which can induce the improvement of enzyme activity in small intestine, advance the development of small intestine digestion function, promote health and improve its production performance, and promote the digestion and absorption of nutrient substances.

Superfine cockroach ootheca powder: high-quality insect protein feed and contains chitin for improving immunity.

The invention mixes the water extract, alcohol extract, zymolyte, and superfine powder of periplaneta egg sheath of America together according to scientific proportion, wherein the water extract is rich in functional amino acids, the alcohol extract is rich in lobster creatnine, protocatechuic acid glucoside, trigonelline and other components which improve the intestinal immunity, the zymolyte is rich in small peptide substances which improve the intestinal digestion capability, and the superfine powder of the periplaneta egg sheath is used as high-quality insect protein feed and chitin.

The invention has the beneficial effects that: the feed additive is natural in raw materials and can replace antibiotics (nosiheptide), and the additive can enhance the natural immunity of animal organisms, prevent the occurrence of animal epidemic diseases, promote the digestion and absorption of nutrient substances and promote the growth of animals when added into the feed.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to specific embodiments below.

The extraction method of the periplaneta americana aqueous extract comprises the following steps: the method comprises the following steps of drying periplaneta americana by microwave, crushing and sieving with a 80-mesh sieve, wrapping with cloth, soaking with distilled water for 3 hours according to the mass ratio of 1:10, rapidly heating to boil, decocting with soft fire for 1 hour, naturally cooling to room temperature, concentrating at low temperature (less than or equal to 50 ℃) in a rotary evaporator, drying, and grinding into powder to be sieved with the 80-mesh sieve.

The extraction method of the alcohol extract of the periplaneta americana comprises the following steps: mixing 70% ethanol and Periplaneta americana powder (prepared by microwave drying and pulverizing Periplaneta americana and sieving with 80 mesh sieve) at a liquid-material ratio of 40m L:1g, standing, soaking for 4h, extracting at 61.4 deg.C for 45min, concentrating at low temperature (less than or equal to 50 deg.C) in a rotary evaporator, oven drying, and grinding into powder and sieving with 80 mesh sieve.

The preparation method of the zymolyte of the periplaneta americana comprises the following steps:

1) periplaneta americana powder (microwave drying Periplaneta americana, pulverizing, sieving with 80 mesh sieve) by supercritical CO₂Extracting to remove grease to obtain degreased cockroach protein powder;

2) weighing degreased cockroach protein powder, adding water to prepare a 10% solution, adding a sodium hydroxide solution to adjust the pH value to 7.5, quickly adding prepared Alcalase 2.4L alkaline endoprotease liquid (the enzyme activity is more than or equal to 10 ten thousand U/g) according to the mass ratio of 0.5%, heating to 50-55 ℃ for enzymolysis for 2 hours, and maintaining the pH value of the solution to be 7.5 constant in the enzymolysis process;

3) inactivating enzyme after enzymolysis, centrifuging at 8000r/min, and freeze drying the supernatant to obtain small peptide powder.

Example 1

The mixture ratio (weight percentage) is as follows: the composition comprises 10% of periplaneta americana aqueous extract, 0.4% of periplaneta americana alcohol extract, 14% of periplaneta americana zymolyte, 19% of periplaneta americana ootheca superfine powder and the balance of a carrier (zeolite powder).

The preparation method comprises the following steps: the raw materials are mixed evenly for use.

Example 2

The mixture ratio (weight percentage) is as follows: 11% of periplaneta americana aqueous extract, 0.5% of periplaneta americana alcohol extract, 15% of periplaneta americana zymolyte, 20% of periplaneta americana ootheca superfine powder and the balance of a carrier (zeolite powder).

The preparation method comprises the following steps: the raw materials are mixed evenly for use.

Example 3

The mixture ratio (weight percentage) is as follows: 7% of periplaneta americana aqueous extract, 0.3% of periplaneta americana alcohol extract, 12% of periplaneta americana zymolyte, 16% of periplaneta americana ootheca superfine powder and the balance of a carrier (zeolite powder).

The preparation method comprises the following steps: the raw materials are mixed evenly for use.

Examples of the experiments

Broiler comparative experiments were conducted in the poultry institute laboratory, institute of agricultural sciences, Shandong province. 240 fast-growing white feather broilers of the same variety, from the same hatchery and 1 day old are randomly divided into 2 groups, 6 groups are repeated, 20 broilers are repeated for each group, the broilers are respectively raised in two identical environmental conditions (15W light source, 24 hours of illumination in the first two days, 23 hours later, brooding temperature of 1-2 days old is 34-35 ℃, 3 ℃ is reduced in the next 7 days, the temperature is reduced to about 21 ℃ in the 28 th day and is kept until the test is finished, 65% in the first two weeks and 55% in the later, and the chickens with the same area (30 square meters) are kept in the barns, the ration ratio of the two groups is shown in table 1, 0.1% of the additive (formula of example 1) is added in the test group, 0.1% of nosiheptide is added in the control group. The disease and death conditions are recorded in the test process, the weight is weighed after the test is finished, the feed intake is counted, the material-weight ratio is calculated, and the test result is shown in table 2.

TABLE 1 daily ration formula for experiments

TABLE 2 results of the experiment

As can be seen from Table 2, the differences in average body weight, feed intake and feed conversion ratio were not significant in the two groups. The experimental result shows that the invention has the same effect with the nosiheptide drug additive.