阅读说明：本技术 两种能够缓解甲氧咪草烟对津稻372药害的植物生长调节剂及其应用 (Two plant growth regulators capable of relieving imazamox phytotoxicity to Jindao 372 and application thereof ) 是由 董立尧 项秉晗 于佳星 于 2021-02-02 设计创作，主要内容包括：本发明公开了两种能够缓解甲氧咪草烟对津稻372药害的植物生长调节剂及其应用,该植物生长调节剂具有显著缓解甲氧咪草烟对津稻372的药害,同时不影响其对杂草稻的除草效果；显著降低用药量,降低化学防除成本等优点。是两种目前能有效缓解甲氧咪草烟对津稻372产生的药害,同时不降低其对杂草稻除草效果的可行的植物生长调节剂。(The invention discloses two plant growth regulators capable of relieving the phytotoxicity of imazamox on Jindao 372 and application thereof, wherein the plant growth regulators have the advantages of remarkably relieving the phytotoxicity of imazamox on Jindao 372 and simultaneously not influencing the weeding effect of the imazamox on weedy rice; obviously reducing the dosage, reducing the chemical prevention and control cost and the like. Is two feasible plant growth regulators which can effectively relieve the phytotoxicity of the imazamox to the Jindao 372 and simultaneously do not reduce the weeding effect of the imazamox to the weedy rice.)

1. Two plant growth regulators capable of relieving the phytotoxicity of imazamox on Jindao 372 and application thereof are characterized in that the phytotoxicity of imazamox on Jindao 372 can be relieved by using Binbu and Yuexiang, and the weeding effect of the imazamox on weedy rice is not influenced.

2. The composition as claimed in claim 1, wherein the dose range of said prophy is 45-90g a.i./ha, the dose range of said prophy is 450-900ml/ha, and the dose range of said imazamox is 50-100g a.i./ha.

3. The compound of claim 1, wherein 45g of a.i./ha benezetime, 450ml of/ha benezetime and imazamox are compounded, which is effective in alleviating the phytotoxicity of 50g of a.i./ha imazamox on jin rice 372. The 450-ml/ha-Yue-Bao mixed with the imazamox can effectively relieve the phytotoxicity of 100g of a.i./ha-imazamox to the Jindao 372.

4. The method as claimed in claim 1, wherein the application of 450 ml/ha-care or 90g a.i./ha-care can alleviate the phytotoxicity of 50g a.i./ha-imazethapyr to jin rice 372.

5. The method as claimed in claim 1, wherein the application method comprises spraying stem and leaf, mixing and combining at 2 leaf stage after seedling stage of Jindao 372, and performing stem and leaf treatment on weedy rice and Jindao 372.

6. The method as claimed in claim 1, wherein the application method comprises spraying stem leaves of Jinda 372 having caused the imazamox phytotoxicity on Binbu and Yue stems after 2-leaf stage of Jinda 372 emergence of phytotoxicity caused by the imazamox phytotoxicity.

Technical Field

The invention belongs to the technical field of plant protection, and relates to two plant growth regulators, namely Binbu and Yuexiang, which are used for relieving phytotoxicity of imazamox on Jindao 372.

Background

Rice is one of the most important food crops in the world, and rice is the staple food in more than 65% of the global population. China is the country with the highest total rice yield, and among numerous grain crops, the total rice yield is the first, which plays a significant role in food production in China.

In recent years, the rice cultivation mode and the cultivation system are respectively changed into direct seeding mode, no-tillage mode and shallow tillage mode, so that weedy rice is seriously generated. Weedy rice and cultivated rice belong to the same species, so that no herbicide can prevent and kill the weedy rice and is safe for the rice at present. In order to solve the problem of the flood disaster of weedy rice in direct seeding fields, crop research institute in Tianjin city successfully cultivates rice varieties resistant to imidazolinone herbicides such as Jinza rice 372 and Jinkuji 818, and establishes a set of prevention and control strategies for preventing and killing weedy rice by matching weedy-resistant rice with broad-spectrum herbicides.

Imazamox belongs to an ALS (acetolactate synthase) inhibitor herbicide, is a post-emergence stem and leaf treatment agent, and is mainly used for preventing and killing annual gramineae and broadleaf weeds in soybean fields. After the weeds are absorbed by stems and leaves, the activity of ALS in vivo is inhibited, so that the biosynthesis of branched chain amino acid is inhibited, the synthesis of protein is inhibited, the cell division is inhibited, and the normal growth of plants is inhibited to finally cause the death of the plants. Because of the advantages of weeding effect on weedy rice, relatively short soil residual period, high activity, safety to people and livestock and the like, the weedy rice herbicide is developed by partial enterprises to be a herbicide for preventing and killing weedy rice by matching with rice varieties resistant to imidazolinone herbicides. In the laboratory preliminary test, however, it was found that high doses of imazamox, an imidazolinone herbicide, were phytotoxic to jin rice 372 seedlings, and therefore, it was necessary to alleviate phytotoxicity by applying a plant growth regulator.

The plant growth regulator has the advantages that the plant growth and development can be influenced and the generation of herbicide phytotoxicity can be relieved by changing the balance of endogenous hormone signal molecules of plants. The product contains various natural plant endogenous hormones, flavonoid catalytic equilibrium components, amino acid compounds, stress tolerance inducers and the like, and can induce crops to improve stress tolerance, increase yield, improve quality, relieve phytotoxicity and the like. The yuanfu seaweed fertilizer is produced by taking seaweed extract as a raw material through a multiple fermentation process, is rich in a large amount of trace elements required by plant growth and a plurality of small molecular active substances such as amino acid, oligosaccharide, polyamine, acid, phenol and the like, can promote the metabolism of crops, stimulate the potential of the crops, increase the yield, improve the stress resistance of the crops and relieve the phytotoxicity caused by herbicide.

According to the invention, through the blending of imazamox and the protection and spraying of the protection after phytotoxicity, the intensive research is carried out, and the results show that the protection and the protection have no influence on the weeding effect of the imazamox on the weedy rice in the direct-seeding rice field, and the phytotoxicity of the imazamox on the Jindao 372 is obviously reduced.

The invention aims to reduce the phytotoxicity of the herbicide on Jindao 372 by mixing the herbicide and the plant growth regulator or spraying the plant growth regulator after the herbicide generates phytotoxicity.

The invention realizes the aim through the following technical scheme:

the herbicide is mixed with a plant growth regulator, or the plant growth regulator is sprayed after the herbicide generates phytotoxicity, and the main components of the herbicide are the herbicide imazamox and the plant growth regulator which are used for protecting and protecting, so that the phytotoxicity of the imazamox to the Jindao 372 is relieved.

The compounding and combination use method of the imazamox and the Bifen and the Yue-Hu comprises the following steps: spraying stem and leaf at 2 leaf stage after seedling of Jindao 372.

The application method of spraying the imazamox to protect the pesticide hazard comprises the following steps: and (3) spraying stem leaves after 5 days of imazamox spraying for manifestation at 2 leaf stage after 372 seedling of Jindao.

The imazamox is mixed with the pesticide protection and the protection, and the pesticide protection and the protection after the imazamox generates the pesticide damage is sprayed on the imazamox, so that the advantages of:

(1) can obviously relieve the phytotoxicity of the imazamox to the Jindao 372.

(2) After the Bifen and the Yue-Feng are added, the imazamox has no influence on the weeding effect of the weedy rice.

Detailed Description

(one) effect of mixing of Bifen, Yue Huo and imazamox on weeding effect of weedy rice-laboratory test:

the laboratory tests were conducted in a laboratory sunlight greenhouse from 5 months 2020 to 6 months 2020.

Reagent to be tested: 4% Methoxyfenamic acid tobacco SC (Meifeng agrichemical Co., Ltd.)

Bibao (Germany Agefu agricultural and forestry environment biotechnology GmbH)

Yue Hu (American Saiduo Mei science and technology Co., Ltd.)

The tested weeds: weed rice (11 months in 2014, three-kitchen village in Funing county, salt city, Jiangsu province)

Laboratory test procedure

1. Test reagent for influence of mixing of Bifen, Yue Feng and imazamox on weeding effect of weedy rice and dosage setting of auxiliary agent

1.1 compounding of Bifen, Yue Huo and Mimex Mexican to effect weed rice in the weeding effect test the dose settings are as follows:

the dose of imazamox is as follows: 50. 100g a.i./ha.

The dose of the benCare is as follows: 0. 45 and 90 g/ha.

The amount of the tonic: 0. 450g/ha and 900g/ha

The imazamox is mixed with the Binban and Yue Hu in pairs, and each treatment is repeated for 4 times.

2. Test process for influence of mixed application of Bifen, Yuehuo and imazamox on weeding effect of weedy rice

The effect of the mixture of Bifen, Yue Huo and imazamox on the weeding effect of the weedy rice is tested by adopting an indoor whole plant bioassay method.

15 weed rice seeds are sown in square plastic pots (7cm multiplied by 7cm) with 3/4 at the bottom and containing mixed soil (clay soil: organic matter 1: 2) with the pH value of 4.5 and the organic matter content of 1.4%, and experiments are carried out under the condition of simulating direct sowing in dry paddy fields. The plastic pot absorbs water from the bottom until the soil is saturated, and then the plastic pot is placed in a greenhouse for culturing (the temperature is about 30 ℃ in the daytime, the temperature is about 20 ℃ in the nighttime, and the natural illumination is performed). And (5) when the seedlings grow to the 1-leaf stage, setting seedlings to 8 plants in each pot. When the leaves grow to 2 leaves, the stems and leaves are sprayed with corresponding dosage. The spraying adopts a 3WP-2000 type walking type bioassay spraying tower produced by Nanjing agricultural mechanization research institute of agricultural department, the rotating speed of a main shaft is 96mm/r, the spraying height is 300mm, the effective spraying width of a nozzle is 350mm, the flow rate of the nozzle is 390mL/min, the walking distance is 1340mm, and the volume of liquid medicine is 30 mL. After treatment, the seeds are continuously put into a sunlight greenhouse for growth.

And (5) surveying and recording the fresh weight of the weedy rice in each plastic pot 21 days after the application of the herbicide, and calculating the fresh weight inhibition rate.

Fresh weight inhibition ratio (% control fresh weight-treated fresh weight)/control fresh weight × 100%

The results of the laboratory tests are shown in the following table:

TABLE 1 Bifen Effect on imazamox herbicidal Effect on weedy rice

^aThe letters indicate the significant difference between the different treatments for each column of data (Duncan P < 0.05).

TABLE 2 Effect of Yuenfang on the herbicidal Effect of imazamox on weedy rice

As can be seen from tables 1 and 2, in the case of the application of the mixture of propamocarb, propamocarb and imazamox, the weeding effect on weedy rice at the doses of 50g a.i./ha and 100g a.i./ha cannot be remarkably reduced by the low-concentration and high-concentration propamocarb and the propamocarb. From the aspect of the fresh weight inhibition rate of the overground part, compared with the single imazamox pesticide, when the pesticide, the bactericide and the imazamox are mixed at different concentrations, the fresh weight inhibition rate of the weedy rice after the pesticide is reduced, but the difference is not obvious, the fresh weight inhibition rates are all 88-95 percent, and the requirements of the imazamox on the prevention and removal of the weedy rice in the field are met. Therefore, under indoor conditions, the Binburg and the Yue protection can not reduce the weeding effect of the imazamox on the weedy rice.

(II) blending of Bifen, Yue Hu and imazamox to relieve the phytotoxicity test of the imazamox to the Jindao 372-indoor test:

the indoor experiments were performed in the sunlight greenhouse of the laboratory from 6 months to 7 months of 2020.

Reagent to be tested: 4% Methoxyfenamic acid tobacco SC (Meifeng agrichemical Co., Ltd.)

Test safeners: bibao (Germany Agefu agricultural and forestry environment biotechnology GmbH)

Yue Hu (American Saiduo Mei science and technology Co., Ltd.)

Test work: jindao 372 (purchase in Jiangsu three-good breed in 2018)

Laboratory experiment process

1. Blending of Bifen, Yue Feng and imazamox for relieving phytotoxicity test medicament dose setting of imazamox to Jindao 372

1.1 compounding of Bifen, Yue Hu and Imazethapyr dose settings for toxicity test of Imazepyr on jin Rice 372 are as follows:

the dose of imazamox is as follows: 0. 50, 100g a.i./ha.

The dose of the benCare is as follows: 0. 45 and 90 g/ha.

The amount of the tonic: 0. 450g/ha and 900g/ha

The imazamox is mixed with the Binban and Yue Hu in pairs, and each treatment is repeated for 4 times.

2. Blending of Bifen, Yue Feng and imazamox for relieving phytotoxicity test process of imazamox on Jindao 372

The phytotoxicity test of the imazamox on the Jindao 372 is carried out by adopting an indoor whole-plant bioassay method.

15 jin rice 372 seeds were sown in square plastic pots (7 cm. times.7 cm) with 3/4-degree mixed soil (clay soil: organic matter: 1: 2) with pH 4.5 and organic matter content of 1.4% perforated at the bottom, and the experiment was performed under conditions simulating dry direct seeding in paddy fields. The plastic pot absorbs water from the bottom until the soil is saturated, and then the plastic pot is placed in a greenhouse for culturing (the temperature is about 30 ℃ in the daytime, the temperature is about 20 ℃ in the nighttime, and the natural illumination is performed). And (5) when the seedlings grow to the 1-leaf stage, setting seedlings to 8 plants in each pot. When the leaves grow to 2 leaves, the stems and leaves are sprayed with corresponding dosage. The spraying adopts a 3WP-2000 type walking type bioassay spraying tower produced by Nanjing agricultural mechanization research institute of agricultural department, the rotating speed of a main shaft is 96mm/r, the spraying height is 300mm, the effective spraying width of a nozzle is 350mm, the flow rate of the nozzle is 390mL/min, the walking distance is 1340mm, and the volume of liquid medicine is 30 mL. After treatment, the seeds are continuously put into a sunlight greenhouse for growth.

After the medicine is applied for 21 days, the fresh weight of the Jindao 372 in each plastic pot is recorded in an investigation mode, and the fresh weight inhibition rate and the detoxifying effect of the green and beautiful protection are calculated.

Fresh weight inhibition ratio (% control fresh weight-treated fresh weight)/control fresh weight × 100%

The detoxification effect (E) [ (fresh weight of rice after treatment with herbicide-fresh weight of rice after treatment with herbicide alone)/fresh weight of rice of blank control ] × 100%

The results of the laboratory tests are shown in the following table:

TABLE 3 Bifen action on alleviation of prometryn-372 phytotoxicity

TABLE 4 reduction of Cimetyl-Guerin induced by Cimetyl-Guerin

As is clear from tables 5 and 6, when Bifen, Yue Feng and Mexican were applied simultaneously at the 2-leaf stage, the effect of alleviating the phytotoxicity of Jindao 372 caused by 50g or 100g of a.i./Haimazan tobacco was exhibited.

When the pesticide is added, 45g/ha of the pesticide can effectively relieve the phytotoxicity of imazamox to the Jindao 372 when the using dose of the imazamox is 50g a.i./ha, the fresh weight inhibition rate is 4.76 percent, the fresh weight inhibition rate is obviously lower than 12.62 percent of the fresh weight inhibition rate of the imazamox to the Jindao 372 when the 50g a.i./ha of the imazamox is singly applied, and the detoxification effect is 7.99 percent. Although the fresh weight inhibition rate of the Jindao 372 after 90g/ha Bibao is added has no significant difference with the fresh weight inhibition rate of the Jindao 372 generated by 50g of a.i./ha methoxyfenamic tobacco, the detoxification effect is 3.29 percent, and the phytotoxicity of the 50g of a.i./ha methoxyfenamic tobacco to the Jindao 372 can be relieved. After 45g/ha and 90g/ha of Bifen is added, the fresh weight inhibition rate of the Jindao 372 is obviously higher than that of the Jindao 372 by singly applying imazethapyr, and the phytotoxicity of 100g of a.i./ha imazethapyr to the Jindao 372 can be effectively relieved. Therefore, the pesticide protection can relieve the pesticide damage of the imazamox to the Jindao 372.

When the euphorbia is added, 450g/ha euphorbia can effectively relieve the phytotoxicity of the imazamox tobacco to the Jinmo rice 372 when the dosage is 50g a.i./ha, the fresh weight inhibition rate is 8.73 percent, is obviously lower than 12.62 percent of the fresh weight inhibition rate of the imazamox tobacco to the Jinmo rice 372 when the imazamox tobacco is singly applied, and the detoxification effect is 3.92 percent. While the fresh weight inhibition rate of Jindao 372 produced by the addition of 900g/ha euphorbia and the application of 50g a.i./ha methoxseed alone had no significant difference. After 450g/ha and 900g/ha of the amikax 100 g.i./ha, the fresh weight inhibition rate of the amikax 372 is obviously higher than that of the amikax 372 singly applied, the chemical injury of the amikax 100 g.i./ha on the jin rice 372 can be effectively relieved, the fresh weight inhibition rates are obviously reduced and respectively reach 10.02% and 13.78%, and the detoxification effects are respectively 26.18% and 22.41%. Thus, 450g/ha may alleviate the phytotoxicity of 50-100g of a.i./ha imazapic on jin rice 372, while 900g/ha may alleviate the phytotoxicity of 100g of a.i./ha imazapic on jin rice 372.

(III) topdressing and protecting the Bifen and Yue-Protect to relieve the toxicity test-indoor test of the Jindao 372 caused by the imazamox:

the indoor experiments were carried out in the sunlight greenhouse of the laboratory from 8 months to 9 months of 2020.

Reagent to be tested: 4% Methoxyfenamic acid tobacco SC (Meifeng agrichemical Co., Ltd.)

Test safeners: bibao (Germany Agefu agricultural and forestry environment biotechnology GmbH)

Yue Hu (American Saiduo Mei science and technology Co., Ltd.)

Test work: jin paddy 372 (purchase in Jiangsu three good breed in 2018)

Laboratory experiment process

1. Topdressing and protecting the dose setting of pesticide for removing toxicity test of Jindao 372 caused by imazamox

1.1 topdressing and Care the dose settings for the phytotoxicity test of JinDai 372 caused by Mexican tobacco were as follows:

the dose of imazamox is as follows: 50. 100g a.i./ha.

Safener and safener dosages: 45. 90 g/ha.

The amount of the tonic: 450. 900g/ha

2. Test process of topdressing Binbu and Yue protection for relieving phytotoxicity of Jindao 372 caused by imazamox

The Bifen and Yue protection dressing test is used for testing the elimination of the injury of Jindao 372 caused by imazamox by adopting an indoor whole-plant bioassay method.

15 jin rice 372 seeds were sown in square plastic pots (6 cm. times.6 cm) with 3/4-degree mixed soil (clay soil: organic matter: 1: 2) with pH 4.5 and organic matter content of 1.4% perforated at the bottom, and the experiment was performed under conditions simulating dry direct seeding in paddy fields. The plastic pot absorbs water from the bottom until the soil is saturated, and then the plastic pot is placed in a greenhouse for culturing (the temperature is about 30 ℃ in the daytime, the temperature is about 20 ℃ in the nighttime, and the natural illumination is performed). And (5) when the seedlings grow to the 1-leaf stage, setting seedlings to 8 plants in each pot. When the leaves grow to the 2-leaf stage, spraying imazamox with corresponding dose on the stems and leaves. The spraying adopts a 3WP-2000 type walking type bioassay spraying tower produced by Nanjing agricultural mechanization research institute of agricultural department, the rotating speed of a main shaft is 96mm/r, the spraying height is 300mm, the effective spraying width of a nozzle is 350mm, the flow rate of the nozzle is 390mL/min, the walking distance is 1340mm, and the volume of liquid medicine is 30 mL. After treatment, the seeds are continuously put into a sunlight greenhouse for growth. After the symptoms appear 5 days after the application, the patient is respectively applied with the Binbu and Yue protection of each concentration.

And (4) surveying and recording the fresh weight of the Jindao 372 in each plastic pot 21 days after the medicine is applied, and calculating the fresh weight inhibition rate.

Fresh weight inhibition ratio (% control fresh weight-treated fresh weight)/control fresh weight × 100%

The detoxification effect (E) is that (fresh weight of rice after being treated by the Binbao and Yue ben plus herbicide-fresh weight of rice after being treated by the herbicide alone)/blank

Fresh weight of rice as a control is 100%

The results of the laboratory tests are shown in the following table:

TABLE 5 topdressing for relieving the phytotoxicity of jin rice 372

TABLE 6 dressing and protecting the relief of the symptomatic JinDai 372 phytotoxicity

As can be seen from tables 5 and 6, after spraying 50g of the a.i./ha methoxyimazethapyr pesticide at the 2-leaf stage, it was found that the jin rice 372 became green (5 d after the pesticide), and then the topdressing was performed for protection and protection, which has significant effect of alleviating the phytotoxicity of 50g of the a.i./ha methoxyimazethapyr to the jin rice 372, but has no significant effect of alleviating the phytotoxicity of 100g of the a.i./ha methoxyimazethapyr to the jin rice 372.

When the topdressing is carried out on the composite, 90g/ha of the composite can obviously relieve the phytotoxicity of 50g of a.i./ha methoxyfenamic tobacco to the Jindao 372, the fresh weight inhibition rate is 7.26%, and the detoxification effect is 5.49%. While the 100g of the a.i./haoxymatrine had no effect of alleviating the phytotoxicity of Jindao 372. The fresh weight inhibition ratio of the treatment group to which 45g/ha protection was applied was not significantly different from that of the treatment group to which only 50, 100g of a.i./ha imazethapyr was applied. Therefore, when the Jindao 372 is found to have the phenomenon of green leaf apex, the phytotoxicity of 50g of a.i./ha imazamox tobacco on the Jindao 372 can be remarkably relieved by applying 90g/ha Biben.

When the dressing is applied to the Yanghe, 450g/ha Yanghe can obviously relieve the phytotoxicity of 50g a.i./ha methoxyfenamic tobacco to the Jindao 372, the fresh weight inhibition rate is 2.07 percent, and the detoxification effect is 10.66 percent. While the 100g of the a.i./haoxymatrine had no effect of alleviating the phytotoxicity of Jindao 372. The fresh weight inhibition ratio of the treatment group to which 900g/ha care was applied was not significantly different from that of the treatment group to which only 50, 100g of a.i./ha methoxyimazethapyr was applied. Therefore, when the Jindao 372 is found to have the phenomenon of green leaf apex, the 450g/ha synergistic effect can remarkably relieve the phytotoxicity of 50g of a.i./ha imazamox tobacco on the Jindao 372.