阅读说明：本技术 一种提高注射脂肪的成活量的方法 (Method for improving survival rate of injected fat ) 是由 闵守平 唐广正 于 2021-01-26 设计创作，主要内容包括：本发明公开了一种提高注射脂肪的成活量的方法,包括以下步骤,S1,采用标准的胶原酶技术,从脂肪中获取基质血管细胞群与间冲质干细胞,S2,用细胞计数器计算干细胞数量,并记录注射的脂肪的位置与脂肪的细胞的数量,S3,将基质血管细胞群与间冲质干细胞进行注射,S4,术后,每隔一段时间,对脂肪容量进行检测,并进行记录,所述S1中,在将基质血管细胞群与间冲质干细胞冲入到脂肪内时,先在间冲质干细胞培养基内加入适量的血清,并将其培养3-5个小时。在本发明实施过程中,具有促使血运血供快速恢复,术后不淤青不肿胀,修复手术中因为提取剥离所破坏的脂肪组织,提升术后自身免疫,降低感染等并发生的出现,作为脂肪成活的载体,提高脂肪移植的成活率等优点。(The invention discloses a method for improving survival of injected fat, which comprises the following steps of S1, obtaining stromal vascular cell group and mesenchymal stem cells from fat by adopting a standard collagenase technology, S2, calculating the number of the stem cells by using a cell counter, recording the position of the injected fat and the number of the cells of the fat, S3, injecting the stromal vascular cell group and the mesenchymal stem cells, S4, detecting and recording the fat volume at intervals after operation, and in S1, when the stromal vascular cell group and the mesenchymal stem cells are flushed into the fat, firstly adding a proper amount of serum into a mesenchymal stem cell culture medium and culturing the serum for 3-5 hours. In the implementation process of the invention, the invention has the advantages of promoting the rapid recovery of blood supply, causing no blood stasis and swelling after operation, repairing the fat tissue damaged by extraction and stripping in the operation, improving the autoimmunity after operation, reducing the occurrence of infection and the like, serving as a carrier for fat survival, improving the survival rate of fat transplantation and the like.)

1. A method of increasing the survival of injected fat, comprising: comprises the following steps of (a) carrying out,

s1, obtaining stromal vascular cell population and mesenchymal stem cells from fat by adopting a standard collagenase technology,

s2, counting the number of stem cells by using a cell counter, recording the position of the injected fat and the number of fat cells,

s3, injecting the stromal vascular cell population and mesenchymal stem cells,

s4, detecting and recording the fat volume at intervals after the operation.

2. The method of claim 1, wherein the step of increasing the survival rate of injected fat comprises: in S1, when the stromal vascular cell population and mesenchymal stem cells are flushed into fat, a suitable amount of serum is added to the mesenchymal stem cell culture medium and cultured for 3 to 5 hours.

3. The method of claim 1, wherein the step of increasing the survival rate of injected fat comprises: in S1, 35ml of the mechanical vascular cell population and 15ml of adipose-derived mesenchymal stem cells were obtained from 50ml of fat.

4. The method of claim 1, wherein the step of increasing the survival rate of injected fat comprises: in S4, when the fat volume of the patient is detected, a detection cycle is set every 5-10 days, and the time for each detection is set to be a different time point, and the change of the fat volume obtained from each detection is recorded.

5. The method of claim 4, wherein the step of increasing the survival rate of injected fat comprises: the detection method is scanning detection.

6. The method of claim 4, wherein the step of increasing the survival rate of injected fat comprises: in S4, the detection is performed for at least 10 cycles.

Technical Field

The invention belongs to the technical field of cosmetology, and particularly relates to a method for improving survival rate of injected fat.

Background

Mesenchymal stem cells are pluripotent stem cells that have all of the commonalities of stem cells, namely self-renewal and multipotential differentiation capacity. The application is most in clinical application, and the combined application with hematopoietic stem cells can improve the success rate of transplantation and accelerate hematopoietic reconstruction. Mesenchymal stem cells are a research branch of stem cells, a type of cells having the ability to self-replicate and differentiate in multiple directions, which can constantly self-renew and under specific conditions transform into one or more cells constituting a tissue or organ of the human body. Stem cells are the cells of origin of the body and are the progenitor cells that form various tissues and organs of the human body.

In the technical field of cosmetology, people are particularly interested in face maintenance, particularly in filling fat into the face to increase the fullness of the face.

Disclosure of Invention

In view of the above situation, in order to overcome the defects of the prior art, the invention provides a method for improving the survival rate of injected fat, which effectively solves the problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: a method for increasing the survival rate of injected fat comprises the following steps,

s1, obtaining stromal vascular cell population and mesenchymal stem cells from fat by adopting a standard collagenase technology,

s2, counting the number of stem cells by using a cell counter, recording the position of the injected fat and the number of fat cells,

s3, injecting the stromal vascular cell population and mesenchymal stem cells,

s4, detecting and recording the fat volume at intervals after the operation.

Preferably, in S1, when the stromal vascular cell population and mesenchymal stem cells are flushed into fat, a suitable amount of serum is added to the mesenchymal stem cell culture medium and cultured for 3 to 5 hours.

Preferably, in S1, 35ml of the mechanical vascular cell population and 15ml of adipose-derived mesenchymal stem cells are obtained from 50ml of fat.

Preferably, in S4, when the fat volume of the patient is detected, a detection cycle is set at intervals of 5-10 days, and the time of each detection is different, the change of the fat volume obtained from each detection is recorded.

Preferably, the detection method is scanning detection.

Preferably, in S4, the detection is performed for at least 10 cycles.

Compared with the prior art, the invention has the beneficial effects that:

in the implementation process of the invention, the invention has the advantages of promoting the rapid recovery of blood supply, causing no blood stasis and swelling after operation, repairing the fat tissue damaged by extraction and stripping in the operation, improving the autoimmunity after operation, reducing the occurrence of infection and the like, serving as a carrier for fat survival, improving the survival rate of fat transplantation and the like.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention discloses a method for improving the survival amount of injected fat, which comprises the following steps,

firstly, a stromal vascular cell population and mesenchymal stem cells are obtained from fat by adopting a standard collagenase technology, a mechanism vascular cell population of 35ml and mesenchymal stem cells of 15ml are obtained from 50ml of fat, the number of the stem cells is calculated by using a cell counter, the position of the injected fat and the number of the cells of the fat are recorded, the stromal vascular cell population and the mesenchymal stem cells are injected, and before and after the operation, the fat volume is detected and recorded at intervals.

In S1, when the stromal vascular cell population and the mesenchymal stem cells are flushed into fat, a proper amount of serum is added into a mesenchymal stem cell culture medium and cultured under proper conditions, the culture process is carried out under aseptic conditions and cultured for 3-5 hours, so that the stem cells are activated, and then the stem cells are injected into the face of a user, so that the stem cells can be rapidly differentiated after entering the human body.

In S4, when the fat volume of the patient is detected, a detection cycle is set every 5-10 days, and the time for each detection is set to be a different time point, and the change of the fat volume obtained from each detection is recorded.

After the stem cells enter the human body, the number of the stem cells and the volume of fat in the human body are detected by scanning detection.

In S4, the detection is performed for at least 10 cycles in total.

The follow-up time lasts for several months, and during the follow-up process, the change of fat volume is continuously detected, taking the example of obtaining 35ml of mechanism vascular cell population and 15ml of adipose-derived mesenchymal stem cells from 50ml of fat, the average fat injection amount is 18.4ml, the average cell number of the stromal vascular cell population which still survives 70 percent is 4.8x10^5, the survival fat amount and the stromal vascular cell population within several months of follow-up process are positively correlated, the age and the stromal vascular cell population are not correlated, and the filling of the adipose-derived mesenchymal stem cells and the stromal vascular cell population on the face can improve the treatment effect

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.