阅读说明：本技术 过滤装置及过滤设备 (Filter device and filter equipment ) 是由 郭秋泉 贾沛沛 赵呈春 杨军 于 2020-12-09 设计创作，主要内容包括：本发明公开了一种过滤装置,包括过滤部,过滤部包括压膜件、过滤件和载膜件；压膜件上设置有第一通液孔,第一通液孔用于使血液穿过压膜件,血液包括CTC和滤液；过滤件上设置有过滤孔,过滤孔的尺寸小于CTC的直径,过滤孔用于使滤液穿过过滤件,且将CTC分离在过滤件上；压膜件固定于载膜件,载膜件上设置有容置槽,过滤件嵌入容置槽,容置槽的底面上设置有第二通液孔,第二通液孔用于使滤液穿过压膜件。过滤件被压膜件限制在了容置槽中,完成CTC的分离操作后,只需整体移动过滤部,即可实现对过滤件位置的改变,有效防止了移取过滤件时出现CTC意外流失的情况发生。本发明还公开了一种具有上述过滤装置的过滤设备。(The invention discloses a filtering device, which comprises a filtering part, wherein the filtering part comprises a membrane pressing piece, a filtering piece and a membrane carrying piece; the membrane pressing piece is provided with a first liquid through hole, the first liquid through hole is used for enabling blood to penetrate through the membrane pressing piece, and the blood comprises CTC and filtrate; the filter element is provided with filter holes, the size of each filter hole is smaller than the diameter of the CTC, and the filter holes are used for enabling the filtrate to pass through the filter element and separating the CTC on the filter element; the membrane pressing piece is fixed on the membrane carrying piece, a containing groove is formed in the membrane carrying piece, the filtering piece is embedded into the containing groove, a second liquid through hole is formed in the bottom face of the containing groove, and the second liquid through hole is used for enabling filtrate to penetrate through the membrane pressing piece. The filtration piece is restricted in the containing groove by the film pressing piece, and after the separation operation of CTC is completed, only the whole filtration part is needed to move, so that the change of the position of the filtration piece can be realized, and the condition that CTC is accidentally lost when the filtration piece is moved is effectively prevented. The invention also discloses a filtering device with the filtering device.)

1. A filter device, comprising:

the filter part comprises a membrane pressing piece, a filter piece and a membrane carrying piece;

the membrane pressing piece is provided with a first liquid through hole, the first liquid through hole is used for enabling blood to pass through the membrane pressing piece, and the blood comprises CTC and filtrate;

the filter element is provided with filter holes, the size of which is smaller than the diameter of the CTC, the filter holes are used for enabling the filtrate to pass through the filter element and separating the CTC on the filter element;

the membrane pressing piece is fixed on the membrane carrying piece, a containing groove is formed in the membrane carrying piece, the filtering piece is embedded into the containing groove, a second liquid through hole is formed in the bottom surface of the containing groove, and the second liquid through hole is used for enabling the filtrate to penetrate through the membrane pressing piece.

2. The filter device according to claim 1, wherein the filter part further comprises a support member inserted into the receiving groove and closely attached to the filter member, and located below the filter member, and the support member is provided with a flow hole for allowing the filtrate to pass through the support member.

3. The filter device according to claim 1, wherein a pore diameter of the first liquid passing hole becomes gradually smaller in a flow direction of the blood, and a minimum pore diameter of the first liquid passing hole is equal to a pore diameter of the second liquid passing hole.

4. The filtration device of claim 1, wherein the filtration pores are elongated pores, and the elongated pores are arranged in a matrix.

5. The filtration device according to claim 4, wherein the elongated holes are divided into a first group and a second group, the first group and the second group are alternately arranged, the inclined angles of the elongated holes in the first group are the same, the inclined angles of the elongated holes in the second group are the same, and the inclined angles of the elongated holes in the first group are different from the inclined angles of the elongated holes in the second group.

6. The filtering device according to claim 1, further comprising a liquid inlet portion, wherein a liquid inlet is disposed at an upper end of the liquid inlet portion, a first connecting hole is disposed at a lower end of the liquid inlet portion, a liquid inlet chamber is disposed in the liquid inlet portion, the liquid inlet chamber is respectively communicated with the liquid inlet and the first connecting hole, and the liquid inlet portion is fixed to the film pressing member through the first connecting hole.

7. The filtering device according to any one of claims 1 to 6, further comprising a liquid outlet portion, wherein a second connecting hole is formed in the upper end of the liquid outlet portion, a liquid outlet cavity is formed in the liquid outlet portion, the liquid outlet cavity is communicated with the second connecting hole, and the liquid outlet portion is fixed to the membrane carrying member through the second connecting port.

8. The filtration device as claimed in claim 7, wherein the bottom end of the liquid outlet part is provided with a discharge port, the discharge port is communicated with the liquid outlet cavity, and the discharge port is used for enabling the filtrate to flow out of the liquid outlet cavity.

9. The filtration device of claim 8, further comprising a control pump, wherein the control pump is provided with a suction port, the suction port is communicated with the discharge port, and the control pump is used for pumping the gas in the liquid outlet cavity.

10. A filtration apparatus characterized by comprising a pipetting device for introducing the blood into the filtration section and the filtration device of any one of claims 1 to 9.

Technical Field

The invention relates to the field of CTC (circulating tumor cell) detection, in particular to a filtering device and filtering equipment.

Background

CTCs are commonly captured by membrane filtration. In the related art, after the filtration is completed, the filter element needs to be moved to other positions for subsequent analysis, and when the filter element is moved, the captured CTCs are accidentally lost, so that the number of CTCs which can be analyzed is small.

Disclosure of Invention

The present invention is directed to solving at least one of the problems of the prior art. Therefore, the invention provides a filtering device which can effectively prevent CTC from accidentally losing.

The invention also provides a filtering device with the filtering device.

In a first aspect, embodiments of the present invention provide a filter device, including:

the filter part comprises a membrane pressing piece, a filter piece and a membrane carrying piece;

the membrane pressing piece is provided with a first liquid through hole, the first liquid through hole is used for enabling blood to pass through the membrane pressing piece, and the blood comprises CTC and filtrate;

the filter element is provided with filter holes, the size of which is smaller than the diameter of the CTC, the filter holes are used for enabling the filtrate to pass through the filter element and separating the CTC on the filter element;

the membrane pressing piece is fixed on the membrane carrying piece, a containing groove is formed in the membrane carrying piece, the filtering piece is embedded into the containing groove, a second liquid through hole is formed in the bottom surface of the containing groove, and the second liquid through hole is used for enabling the filtrate to penetrate through the membrane pressing piece.

According to the embodiment of the invention, the filtering device has at least the following technical effects:

when having guaranteed to filter and can having accomplished the separation of CTC, filter and be restricted by the pressure membrane piece in the storage tank, accomplish the separation operation back of CTC, only need the moving as a whole filter portion, can realize the change to filtering a piece position, the condition emergence that the CTC accident runs off when having effectively prevented to move and get and filter.

According to some embodiments of the filter device of the present invention, the filter part further includes a support member, the support member is inserted into the receiving groove and is closely attached to the filter member, and is located below the filter member, and the support member is provided with a flow hole for allowing the filtrate to pass through the support member.

According to the filter device of some embodiments of the present invention, the first liquid passing hole has a pore diameter gradually decreasing in the flow direction of the blood, and the minimum pore diameter of the first liquid passing hole is equal to the pore diameter of the second liquid passing hole.

According to some embodiments of the filter device of the present invention, the filter holes are elongated holes, and the elongated holes are arranged in a matrix.

According to some embodiments of the filter device of the present invention, the elongated holes are divided into a first group and a second group, the first group and the second group are alternately arranged, the inclined angles of the elongated holes in the first group are the same, the inclined angles of the elongated holes in the second group are the same, and the inclined angles of the elongated holes in the first group are different from the inclined angles of the elongated holes in the second group.

According to some embodiments of the invention, the filtering device further comprises a liquid inlet part, a liquid inlet is arranged at the upper end of the liquid inlet part, a first connecting hole is arranged at the lower end of the liquid inlet part, a liquid inlet cavity is arranged in the liquid inlet part, the liquid inlet cavity is respectively communicated with the liquid inlet and the first connecting hole, and the liquid inlet part is fixed on the film pressing piece through the first connecting hole.

According to some embodiments of the invention, the filtering device further comprises a liquid outlet part, the upper end of the liquid outlet part is provided with a second connecting hole, a liquid outlet cavity is arranged in the liquid outlet part and is communicated with the second connecting hole, and the liquid outlet part is fixed on the membrane carrying piece through the second connecting port.

According to the filtering device provided by some embodiments of the invention, the bottom end of the liquid outlet part is provided with a discharge port, the discharge port is communicated with the liquid outlet cavity, and the discharge port is used for enabling the filtrate to flow out of the liquid outlet cavity.

The filtering device according to some embodiments of the present invention further comprises a regulating pump, the regulating pump is provided with a suction port, the suction port is communicated with the discharge port, and the regulating pump is used for pumping the gas in the liquid outlet cavity.

A second aspect embodiment of the invention provides a filtration apparatus comprising a pipetting device for feeding said blood to said filtration section and a filtration device according to the above-described first aspect embodiment of the invention.

According to the embodiment of the invention, the filtering equipment at least has the following technical effects:

by adopting the filtering device, the CTC which can be collected and analyzed by the filtering equipment has more quantity, and the effective utilization rate of the CTC in the blood is effectively improved.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is an exploded view of a filter assembly according to an embodiment of the present invention;

FIG. 2 is a front view of a filter assembly according to an embodiment of the present invention;

FIG. 3 is a cross-sectional view of the filter assembly of FIG. 2 taken along line A-A;

fig. 4 is a schematic view of an electron microscope imaging of a filter element with elongated filter holes according to an embodiment of the present invention.

Reference numerals: the filter part 100, the membrane pressing part 110, the first liquid through hole 111, the filter part 120, the filter hole 121, the membrane carrier 130, the accommodating groove 131, the second liquid through hole 132, the support part 140, the flow through hole 141, the sealing part 150, the liquid inlet part 200, the liquid inlet chamber 210, the liquid inlet 220, the first connecting hole 230, the liquid outlet part 300, the liquid outlet chamber 310, the second connecting hole 320, the discharge port 330, and the insertion pipe part 340.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.

In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

A filter device according to an embodiment of the present invention is described below with reference to fig. 1 to 4.

The filter device according to the embodiment of the first aspect of the present invention includes a filter portion 100.

Wherein, the filter part 100 comprises a membrane pressing member 110, a filter member 120 and a membrane carrier member 130; the membrane pressing piece 110 is provided with a first liquid through hole 111, the first liquid through hole 111 is used for enabling blood to pass through the membrane pressing piece 110, and the blood comprises CTC and filtrate; the filter element 120 is provided with filter holes 121, the size of the filter holes 121 being smaller than the diameter of the CTCs, the filter holes 121 being adapted to allow the filtrate to pass through the filter element 120 and to separate the CTCs from the filter element 120; the membrane pressing member 110 is fixed to the membrane carrying member 130, the membrane carrying member 130 is provided with a receiving groove 131, the filter member 120 is embedded in the receiving groove 131, the bottom surface of the receiving groove 131 is provided with a second liquid through hole 132, and the second liquid through hole 132 is used for enabling the filtrate to pass through the membrane pressing member 110.

In a specific use process, firstly, the filter element 120 is inserted into the receiving groove 131, then the membrane pressing element 110 is fixed on the membrane carrying element 130, then the blood to be subjected to the CTC separation operation is input from the first liquid through hole 111, the input blood flows into the filter element 120 through the first liquid through hole 111, the CTC in the blood is blocked by the filter element 120 and is remained on the upper surface of the filter element 120, and the filtrate from which the CTC is separated passes through the filter element 120 through the filter hole 121 and flows out of the filter part 100 through the second liquid through hole 132. Finally, when the CTC separation is completed and the separated CTC is to be subsequently analyzed, the entire filter unit 100 is moved to a laboratory bench or the like for analysis, and then, depending on the specific circumstances, it is selected whether to take out the filter member 120 or to add a test reagent or the like to the CTC on the filter member 120 through the first liquid passage hole 111.

Specifically, the filter element 120 is a microporous organic membrane, the filter element 120 may be in a disk shape, a rectangular plate shape, or the like, a plurality of filter holes 121 are dispersedly formed in the filter element 120, the filter holes 121 may be in a circular shape, a strip shape, or the like, a minimum size of the filter holes 121 is required to be smaller than a diameter of CTCs, when blood passes through the filter element 120, CTCs contained in the blood are blocked by the filter element 120, and filtrate containing no CTCs in the blood passes through the filter element 120 through the filter holes 121, thereby achieving a separation target of CTCs.

The film-carrying member 130 is provided with a receiving groove 131, the receiving groove 131 is similar to the filter member 120 in shape and has a size equal to or slightly smaller than that of the filter member 120, so that the filter member 120 is inserted into the receiving groove 131 by interference. The bottom end of the accommodating groove 131 is provided with a through hole, which is the second liquid through hole 132, when the filtrate passes through the filtering element 120 through the filtering hole 121, the filtrate will flow out of the accommodating groove 131 through the second liquid through hole 132, and the second liquid through hole 132 may be any shape capable of allowing the filtrate to pass through the membrane element 130, such as a cylinder or a spiral, which is not repeated herein.

The membrane pressing member 110 can be fixed on the membrane carrying member 130 by means of fasteners or fastener slots, a through hole is formed in the membrane pressing member 110, the through hole is a first liquid passing hole 111, blood flows into the filtering portion 100 from the first liquid passing hole 111, and similarly, the first liquid passing hole 111 can be any shape capable of enabling blood to pass through the membrane pressing member 110, such as cylindrical or spiral shape, which is not repeated herein.

For example, as shown in fig. 1 and 3, the filter member 120 has a disk shape, and the filter pores 121 are circular pores and are distributed on the filter member 120 in a matrix, and the diameter of the filter pores 121 is 7 μm. The lower extreme of pressure membrane piece 110 is cylindricly, and attacks there is the external screw thread, and the storage tank 131 that sets up on year membrane piece 130 is a cylindric groove, attacks the internal thread that has with above-mentioned external screw thread looks adaptation in the upper end of storage tank 131, after filtering piece 120 embedding storage tank 131, presses membrane piece 110 through screw thread and year membrane piece 130 fixed connection, presses membrane piece 110 to play the effect that compresses tightly to filtering piece 120 simultaneously. The first liquid through hole 111 and the second liquid through hole 132 are cylindrical through holes, and when the film pressing member 110 is fixed on the film carrying member 130, the first liquid through hole 111 and the second liquid through hole 132 are coaxially distributed.

Further, press mold piece 110 and year membrane piece 130 all adopt PMMA (ya keli) to make, and this kind of material has high transparency, and the price is lower, and mechanical properties is good, and when carrying out the CTC separation to blood, the user can see through press mold piece 110 and the casing of year membrane piece 130 and observe the condition in the storage tank 131, has optimized and has used experience.

It can be understood that the filter element 120 is limited in the containing groove 131 and is clamped between the film pressing element 110 and the film carrying element 130, and such a design effectively avoids the occurrence of accidental loss of CTCs in the process of moving the filter element 120, and ensures the accuracy of detection. Meanwhile, the filtering part 100 is easy to disassemble and assemble, can flexibly replace parts, and is convenient for later maintenance and repair.

In some embodiments of the present invention, the filter part 100 further includes a support member 140, the support member 140 is inserted into the receiving groove 131 and is closely attached to the filter member 120, and is located below the filter member 120, and the support member 140 is provided with a flow hole 141, and the flow hole 141 is used for allowing the filtrate to pass through the support member 140.

In a specific use process, the supporting member 140 is first inserted into the receiving groove 131, and then the filtering member 120 is inserted into the receiving groove 131, so that the lower surface of the filtering member 120 is attached to the upper surface of the supporting member 140. The filtrate passing through the filter member 120 through the filter holes 121 may pass through the support member 140 through the flow through holes 141 and flow into the second flow through holes 132.

Specifically, as shown in fig. 1 and 3, the shape of the supporting member 140 is similar to the shape of the receiving groove 131 and the filtering member 120, and the size of the supporting member 140 is equal to or slightly larger than the size of the receiving groove 131, so that the supporting member 140 can be inserted into the receiving groove 131 just or with interference. At least one through hole, i.e., a flow hole 141, is formed in the support member 140, the flow hole 141 may have a cylindrical or prismatic shape, and the support member 140 may be made of PMMA or stainless steel, i.e., the support member 140 has good rigidity and can provide good support for the filter member 120. It will be appreciated that, by such a design, the support member 140 can ensure the strength of the filter member 120 during the filtration process when the filter member 120 filters blood, and effectively avoid the filter member 120 from being burst by the hydraulic pressure of the filtrate in the blood.

In some embodiments of the present invention, the first liquid passing hole 111 has a gradually decreasing pore size in the direction of the flow of blood, and the smallest pore size of the first liquid passing hole 111 is equal to the pore size of the second liquid passing hole 132.

Specifically, as shown in fig. 3, the first liquid through hole 111 is in a shape of an inverted frustum, the first liquid through hole 111 has different hole diameters at different depths, and the hole diameter is inversely proportional to the depth, that is, the hole diameter of the first liquid through hole 111 gradually decreases from top to bottom. On the lower surface of the film pressing member 110, the aperture of the first liquid through hole 111 is the smallest, and is the same as the second liquid through hole 132 arranged on the film carrying member 130, and at this time, the first liquid through hole 111 and the second liquid through hole 132 are coaxially distributed, and the lower end of the film pressing member 110 is inserted into the accommodating groove 131 and pressed on the filter member 120. It is understood that when the blood flows in the first through-hole 111, it comes into contact with the filter member 120 guided by the inclined inner wall of the first through-hole 111, and the filtrate from which CTC is separated flows into the second through-hole 132 just after passing through the filter member 120. Such design has effectively reduced the blood that remains in storage tank 131, has improved the collection rate to the CTC in the blood.

Further, the filter part 100 further includes a sealing member 150, the sealing member 150 is annular, the diameter of the outer ring of the sealing member 150 is equal to or slightly larger than the size of the receiving groove 131, and the sealing member can be inserted into the receiving groove 131 just or in an interference manner, and the diameter of the inner ring of the sealing member 150 is larger than the diameter of the second through hole 132, so as to ensure that the filtering liquid flow will not interfere with the filtering liquid flow passing through the filtering member 120 and entering the second through hole 132. The sealing member 150 is disposed between the pressing member 110 and the filter member 120, i.e., the lower end of the pressing member 110 is not directly pressed against the filter member 120 but against the sealing member 150, and the filter member 120 is compressed by the sealing member 150. It will be appreciated that the provision of the seal 150 effectively prevents blood from seeping out of the edge of the filter element 120, and leakage is effectively avoided. Meanwhile, the sealing member 150 is made of elastic material such as rubber or fluorine glue, and the sealing member 150 is disposed between the film pressing member 110 and the filter member 120, so that the filter member 120 can be protected, and the situation that the filter member 120 is damaged due to contact with the film pressing member 110 made of hard material is avoided.

In some embodiments of the present invention, the filtering holes 121 are elongated holes, and the elongated holes are arranged in a matrix.

Specifically, as shown in fig. 4, the filter holes 121 are arranged in a matrix on the filter member 120, and the filter holes 121 have a strip shape having a width smaller than the diameter of CTC and a ratio of the width to the length of 1:4 to 1: 6. For example, when the width of the filtering hole 121 is 7 micrometers, the length of the filtering hole 121 may be 28 micrometers, 35 micrometers, 42 micrometers, or the like. When the length is too long, the mechanical properties of the filter member 120 are poor, and the filter member is easily damaged, and when the length is too short, the filtering properties of the filter holes 121 are poor, and the desired effect cannot be achieved. The effect here is expected to be that the elongated holes are less prone to clogging than conventional circular holes. The matrix arrangement allows the filter holes 121 to be evenly distributed, optimizing the mechanical properties of the filter element 120.

It will be appreciated that the CTC is mostly spherical in shape, so that when the filter holes 121 are circular holes, clogging is likely to occur, and when CTCs having a diameter similar to that of the filter holes 121 are clogged at the filter holes 121, they may be pressed through the filter member 120 by hydraulic pressure, causing the CTCs to be lost. When the filter holes 121 are elongated holes, even if the filter holes 121 are partially blocked, the filtrate can still pass through the filter element 120 from the rest of the filter holes 121, so that the design effectively reduces the frequency of hydraulic pressure rise caused by blockage of the filter holes 121 by CTC, and the CTC is extruded to pass through the filter element 120, and the acquisition rate of the CTC is improved.

In some embodiments of the present invention, the elongated holes are divided into a first group and a second group, the first group and the second group are alternately disposed, the inclined angles of the elongated holes in the first group are the same, the inclined angles of the elongated holes in the second group are the same, and the inclined angles of the elongated holes in the first group are different from the inclined angles of the elongated holes in the second group. .

Specifically, as shown in fig. 4, the elongated holes are divided into two groups, i.e., a first group and a second group. First group includes a row of even rectangular shape hole in interval, these rectangular shape hole all incline towards same direction, the inclination in rectangular shape hole is the same promptly, it is same, the second group includes a row of even rectangular shape hole in interval, these rectangular shape hole all incline towards same direction, the inclination in rectangular shape hole is the same promptly, and the incline direction in the rectangular shape hole in the second group has the incline direction in rectangular shape hole in the first group, the rectangular shape hole is followed clockwise rotation certain angle again in the first group, for example 90, the incline direction in rectangular shape hole is the same in with the second group promptly.

Meanwhile, the first group and the second group are arranged at intervals, namely, a group of second group of elongated holes is required to be arranged between two adjacent groups of first group of elongated holes, and similarly, a group of first group of elongated holes is required to be arranged between two adjacent groups of second group of elongated holes. And the adjacent first group and the second group are arranged in a staggered manner, it can be understood that the distance between the adjacent strip-shaped holes in the first group is the same as the distance between the adjacent strip-shaped holes in the second group, and the staggered arrangement means that the strip-shaped holes in the first group correspond to the gap between the two adjacent strip-shaped holes in the second group, and the strip-shaped holes in the second group correspond to the gap between the two adjacent strip-shaped holes in the first group.

It can be understood that, with such a design, more filter holes 121 can be formed on the filter element 120 with the same area, while ensuring the mechanical strength, so as to effectively increase the porosity, thereby optimizing the filtering efficiency of the filter element 120.

In some embodiments of the present invention, the liquid pressing device further includes a liquid inlet portion 200, the upper end of the liquid inlet portion 200 is provided with a liquid inlet 220, the lower end of the liquid inlet portion 200 is provided with a first connection hole 230, a liquid inlet chamber 210 is disposed in the liquid inlet portion 200, the liquid inlet chamber 210 is respectively communicated with the liquid inlet 220 and the first connection hole 230, and the liquid inlet portion 200 is fixed to the film pressing member 110 through the first connection hole 230.

Specifically, liquid inlet portion 200 adopts materials such as PMMA or stainless steel to make, is provided with feed liquor chamber 210 in feed liquor portion 200, and feed liquor chamber 210 can be a tortuous spiral channel, also can be cylindrical straight passageway, is provided with inlet 220 in the upper end of feed liquor portion 200, and inlet 220 and feed liquor chamber 210 communicate, and the user can carry out the blood that the CTC separated with needs, inputs into feed liquor chamber 210 from inlet 220, and later blood flows to the bottom of feed liquor chamber 210 downwards. The bottom of the liquid inlet chamber 210 is also provided with a through hole, which is the first connection hole 230, the first connection hole 230 is also communicated with the liquid inlet chamber 210, and blood can flow out of the liquid inlet chamber 210 through the first connection hole 230. It is understood that the shape of the liquid inlet portion 200 can be adaptively adjusted according to the shapes and sizes of the liquid inlet chamber 210, the liquid inlet 220, and the first connection hole 230.

During actual use, liquid inlet portion 200 will be fixed on pressure membrane piece 110 through modes such as fastener, or draw-in groove buckle structure, and the lower extreme of liquid inlet portion 200 matches with the upper end of pressure membrane piece 110 this moment for realize fixed connection. Meanwhile, the shape of the first connection hole 230 is the same as the uppermost end of the first liquid through hole 111, and the size of the first connection hole 230 is equal to or slightly smaller than the uppermost end of the first liquid through hole 111, so as to ensure that the blood flowing out of the liquid inlet chamber 210 can flow into the first liquid through hole 111 except for a little blood hanging on the inner wall of the liquid inlet chamber 210.

For example, as shown in fig. 1 to 3, the liquid inlet portion 200 is cylindrical, the liquid inlet chamber 210 inside the liquid inlet portion is a straight cylindrical channel with equal diameter, and the liquid inlet 220 has equal diameter to the bottom surface of the liquid inlet chamber 210. The first connection hole 230 is located at the lowermost end of the liquid inlet chamber 210, and is also cylindrical, but an inner thread is tapped on the inner circumferential wall of the first connection hole 230. In addition, the upper end of the film pressing member 110 is cylindrical, and the side surface of the upper end of the film pressing member 110 is tapped with an external thread matched with the internal thread on the inner peripheral wall of the first connection hole 230, and the size of the upper end of the film pressing member 110 is also matched with the first connection hole 230, so that the liquid inlet portion 200 can be fixedly connected with the film pressing member 110 through the thread. When connected, the first connection holes 230 are coaxially arranged with the first liquid through holes 111.

It can be understood that the liquid inlet part 200 is additionally arranged on the filter part 100, so that when a user adds blood needing to separate CTC into the filter part 100, the operation is more convenient, and the blood is less prone to spilling out of the filter part 100, thereby effectively reducing waste. Meanwhile, the liquid inlet part 200 can store certain blood, so that the blood is conveniently kept stand and filtered.

In some embodiments of the present invention, the liquid-dispensing device further comprises a liquid-dispensing portion 300, the upper end of the liquid-dispensing portion 300 is provided with a second connecting hole 320, a liquid-dispensing cavity 310 is provided in the liquid-dispensing portion 300, the liquid-dispensing cavity 310 is communicated with the second connecting hole 320, and the liquid-dispensing portion 300 is fixed to the film-carrying member 130 through a second connecting port.

Specifically, a liquid outlet cavity 310 is arranged in the liquid outlet portion 300, the liquid outlet cavity 310 may be a zigzag spiral channel or a columnar straight channel, a second connecting hole 320 is arranged at the upper end of the liquid outlet portion 300, and the second connecting hole 320 is communicated with the liquid outlet cavity 310. In practical use, the liquid outlet cavity 310 is fixed to the lower end of the membrane carrying member 130 by a fastening member or a clamping groove and buckling structure, and at this time, the second connecting hole 320 is communicated with the second liquid through hole 132, and the shapes of the second connecting hole 320 and the second liquid through hole 132 may be the same or different, but the size of the second connecting hole 320 is larger than or equal to the size of the second liquid through hole 132, that is, the position where the second liquid through hole 132 is communicated with the second connecting hole 320 can be completely included by the second connecting hole 320, and such design can ensure that the filtrate flowing out of the second liquid through hole 132 can flow into the second connecting hole 320 except a small amount hung on the inner wall of the second liquid through hole 132. It is understood that the shape of the liquid outlet portion 300 can be adaptively adjusted according to the shape and size of the liquid outlet chamber 310 and the second connection hole 320.

For example, as shown in fig. 1 to 3, the liquid outlet portion 300 is cylindrical, and the liquid outlet cavity 310 inside the liquid outlet portion is a straight cylindrical channel with equal diameter. The second connection hole 320 is located at the uppermost end of the liquid outlet chamber 310, and is also cylindrical, and the outer wall of the liquid outlet portion 300 at the second connection hole 320 is tapped with an external thread. In addition, the lower end of the film carrier 130 is provided with a third connection hole (not labeled), which is also cylindrical and has a size matched with the uppermost end of the liquid outlet 300, and the inner wall of the third connection hole is tapped with an internal thread matched with the external thread of the liquid outlet 300 on the outer wall of the second connection hole 320, so that the liquid outlet 300 can be fixedly connected with the film carrier 130 through the thread. When connected, the second connection hole 320 is coaxially arranged with the second through-hole 132.

It can be understood that the liquid outlet 300 is added to the lower end of the membrane supporting member 130, so that the filtrate flowing out of the second through holes 132 can be collected in time, and the filtrate can be used for other tests and can be discarded after the separation operation is finished.

Further, the liquid outlet 300 is made of transparent material such as PMMA, so when the liquid outlet chamber 310 is a chamber which can only be connected with the outside through the second connecting hole 320, the filtrate can be stored in the liquid outlet chamber 310, and at this time, the user can observe the liquid level of the filtrate in the liquid outlet 300 in time, thereby determining the timing of stopping adding blood to the filtering portion 100, and whether the filtrate in the liquid outlet chamber 310 needs to be processed first, and then the operation is continued.

In some embodiments of the present invention, the bottom end of the liquid outlet 300 is provided with a discharge outlet 330, the discharge outlet 330 is communicated with the liquid outlet chamber 310, and the discharge outlet 330 is used for enabling the filtrate to flow out of the liquid outlet chamber 310.

Specifically, as shown in fig. 3, the bottom end of the liquid outlet portion 300 is provided with a through hole, i.e. a discharge port 330, and the discharge port 330 is communicated with the liquid outlet chamber 310, so that the filtrate in the liquid outlet chamber 310 can flow out of the liquid outlet portion 300 through the discharge port 330. Such a design ensures that filtrate does not overflow out of the chamber 310 during CTC acquisition.

Further, the bottom surface of the liquid outlet cavity 310 is an inclined surface, and the lowest end of the inclined surface is provided with the discharge port 330, so that the residual filtrate in the liquid outlet cavity 310 is less.

Further, as shown in fig. 3, a cannula 340 is further provided at the discharge port 330, and the cannula 340 is fixed to the liquid outlet 300 in a cylindrical shape. A channel is provided in cannula member 340 that communicates with vent 330. In practical use, a user can sleeve one end of the liquid guide tube on the insertion tube piece 340, so that the filtrate discharged from the liquid cavity 310 from the discharge port 330 can flow into the liquid guide tube through the insertion tube piece 340, and the other end of the liquid guide tube can be connected with a waste liquid treatment pool or a filtrate collection device and the like, so that the filtrate is more convenient to treat.

In some embodiments of the present invention, a control pump is further included, and the control pump is provided with a suction port, the suction port is communicated with the discharge port 330, and the control pump is used for extracting the gas in the liquid chamber 310.

Specifically, the control pump may be a peristaltic pump or a diaphragm pump, and the air suction port disposed on the control pump is connected to the exhaust port 330 via a conduit or the like, so that when the control pump starts to operate, air in the liquid chamber 310 can be extracted, and thus the air pressure in the liquid chamber 310 is lower than the external atmospheric pressure, so that pressure difference is generated between two sides of the filter element 120, and the filtrate can rapidly pass through the filter element 120, thereby accelerating the efficiency of collecting CTCs.

Further, the power of the regulating pump is controllable. When the control pump is not started, the blood flows from the first liquid through hole 111 to the filter member 120 only under the action of the self gravity, and then the filtrate passes through the filter member 120 under the action of the self gravity; when the regulating pump starts to operate, the air pressure in the liquid outlet cavity 310 can be controlled by changing the power of the regulating pump, so that the purpose of controlling the speed of the filtrate passing through the filter element 120 is achieved.

The filtering apparatus according to the second aspect of the present invention comprises a pipetting device for feeding blood to the filtering portion 100 and the filtering apparatus according to the above-described first aspect of the present invention.

Specifically, the pipetting device may be a pipette, a syringe, a pump, or the like, and is used to input a blood sample to be CTC separated into the first liquid through hole 111 of the squeeze film member 110. By adopting the filtering device, the CTC which can be collected and analyzed by the filtering equipment has more quantity, and the effective utilization rate of the CTC in the blood is effectively improved.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention.