阅读说明：本技术 一种鱼鳞酶解提取小分子胶原蛋白肽的方法 (Method for extracting micromolecular collagen peptide by fish scale enzymolysis ) 是由 李立 郭红星 周尽学 于 2021-02-04 设计创作，主要内容包括：本发明公开了一种鱼鳞酶解提取小分子胶原蛋白肽的方法,所述小分子胶原蛋白肽的提取步骤如下：加入投料鱼鳞重量3％～8％的酶制剂,对预处理后的鱼鳞进行酶解,得到一次胶原蛋白肽酶解液；将滤出的鱼鳞打磨成粉,然后加入加入投料鱼鳞重量3％的酶制剂,得到二次胶原蛋白肽酶解液；将得到的一次胶原蛋白肽酶解液和二次胶原蛋白肽酶解液搅拌混合,然后使用超滤膜进行精滤,将精滤后的胶原蛋白肽液进行蒸煮浓缩,得到胶原蛋白肽浓缩液。通过对鱼鳞进行二次提取,能够提高鱼鳞中胶原蛋白肽的利用率,降低浪费,且通过多种酶协同作用对鱼鳞进行酶解,能够提高鱼鳞的酶解效率。(The invention discloses a method for extracting micromolecule collagen peptide by fish scale enzymolysis, which comprises the following steps: adding an enzyme preparation which accounts for 3-8% of the weight of the fed fish scales, and carrying out enzymolysis on the pretreated fish scales to obtain primary collagen peptidase hydrolyzed solution; grinding the filtered fish scales into powder, and then adding an enzyme preparation which is 3% of the weight of the fed fish scales into the powder to obtain secondary collagen peptidase hydrolyzed solution; and stirring and mixing the obtained primary collagen peptidase hydrolyzed solution and the secondary collagen peptidase hydrolyzed solution, then performing fine filtration by using an ultrafiltration membrane, and performing cooking concentration on the collagen peptide liquid after the fine filtration to obtain a collagen peptide concentrated solution. Through carrying out the secondary to the fish scale and drawing, can improve the utilization ratio of collagen peptide in the fish scale, reduce extravagantly, and carry out the enzymolysis to the fish scale through multiple enzyme synergism, can improve the enzymolysis efficiency of fish scale.)

1. A method for extracting micromolecular collagen peptide by fish scale enzymolysis is characterized by comprising the following steps: the extraction steps of the small molecule collagen peptide are as follows:

the method comprises the following steps: fish scale cleaning

The collected fish scales are poured into cleaning equipment for cleaning, so that impurities adhered to the surfaces of the fish scales can be cleaned;

step two: pretreatment of

Putting the cleaned fish scales into a reaction kettle, adding an alkali solution into the reaction kettle to enable the pH value of the solution in the reaction kettle to be 7-8, stirring for 1-2 hours to remove mucus on the surfaces of the fish scales, adding a sodium bicarbonate solution to remove lipid substances in the fish scales, and cleaning with deionized water;

step three: extracting by enzymolysis

Adding an enzyme preparation which is 3-8% of the weight of the fed fish scales, carrying out enzymolysis on the pretreated fish scales, adding a sodium bicarbonate solution to adjust the pH value to 5-6, then adjusting the temperature to 15-20 ℃, raising the temperature to 85 ℃ after 24 hours of enzymolysis, carrying out enzyme deactivation, and filtering the enzymolysis liquid to obtain primary collagen peptidase hydrolysate;

step four: secondary enzymolysis extraction

Grinding the fish scales filtered out in the third step into powder, adding an enzyme preparation which is 3% of the weight of the added fish scales, performing enzymolysis on the pretreated fish scales, adding a sodium bicarbonate solution to adjust the pH value to 5-6, adjusting the temperature to 15-20 ℃, raising the temperature to 85 ℃ after 24 hours of enzymolysis, deactivating enzyme, and filtering the enzymolysis liquid to obtain secondary collagen peptidase hydrolysate;

step five: purifying and concentrating

Stirring and mixing the primary collagen peptidase hydrolyzed solution and the secondary collagen peptidase hydrolyzed solution obtained in the third step and the fourth step, then performing fine filtration by using an ultrafiltration membrane, and performing cooking concentration on the collagen peptide liquid after the fine filtration to obtain a collagen peptide concentrated solution;

step six: drying

And (3) rapidly drying the collagen peptide concentrated solution by utilizing spray drying to obtain a finished collagen peptide powder product.

2. The method for extracting small-molecule collagen peptide by fish scale enzymolysis, as claimed in claim 1, wherein: and the cleaning equipment in the step one is stirring cleaning and aeration cleaning.

3. The method for extracting small-molecule collagen peptide by fish scale enzymolysis, as claimed in claim 1, wherein: and in the second step, the alkali solution is sodium hydroxide or potassium hydroxide solution.

4. The method for extracting small-molecule collagen peptide by fish scale enzymolysis, as claimed in claim 1, wherein: and in the fourth step, the fish scales are grinded into powder by a fish scale grinding machine.

5. The method for extracting small-molecule collagen peptide by fish scale enzymolysis, as claimed in claim 1, wherein: the enzyme preparation in the third step and the fourth step comprises 3-8 parts of neutral protease, 8-14 parts of papain, 10-16 parts of pepsin, 5-7 parts of trypsin and 3-5 parts of flavourzyme.

6. The method for extracting small-molecule collagen peptide by fish scale enzymolysis, as claimed in claim 5, wherein: the enzyme preparation comprises 3 parts of neutral protease, 8 parts of papain, 10 parts of pepsin, 5 parts of trypsin and 3 parts of flavourzyme.

7. The method for extracting small-molecule collagen peptide by fish scale enzymolysis, as claimed in claim 5, wherein: the enzyme preparation comprises 3-8 parts of neutral protease, 11 parts of papain, 13 parts of pepsin, 6 parts of trypsin and 4 parts of flavourzyme.

8. The method for extracting small-molecule collagen peptide by fish scale enzymolysis, as claimed in claim 5, wherein: the enzyme preparation comprises 8 parts of neutral protease, 14 parts of papain, 16 parts of pepsin, 7 parts of trypsin and 5 parts of flavourzyme.

Technical Field

The invention belongs to the technical field of collagen peptide extraction, and particularly relates to a method for extracting micromolecule collagen peptide by fish scale enzymolysis.

Background

Collagen peptide is an extracellular protein. Protein peptide consisting of two or more amino acids. The absorption of human body is carried out in a peptide mode, and the absorption utilization rate of the edible protein peptide can reach 100%. Collagen is the most important component of the extracellular matrix.

At present, when fish scale enzymolysis is used for extracting collagen peptide in the prior art, most of the collagen peptide in the fish scale is not extracted, so that waste is caused, and the enzymolysis efficiency is low during extraction.

Disclosure of Invention

The invention aims to provide a method for extracting micromolecule collagen peptide by fish scale enzymolysis, which aims to solve the problems that most of collagen peptide in fish scales is not extracted, waste is caused, and the enzymolysis efficiency is low during extraction.

In order to achieve the purpose, the invention provides the following technical scheme: a method for extracting micromolecular collagen peptide by fish scale enzymolysis comprises the following steps:

the method comprises the following steps: fish scale cleaning

The collected fish scales are poured into cleaning equipment for cleaning, so that impurities adhered to the surfaces of the fish scales can be cleaned;

step two: pretreatment of

Putting the cleaned fish scales into a reaction kettle, adding an alkali solution into the reaction kettle to enable the pH value of the solution in the reaction kettle to be 7-8, stirring for 1-2 hours to remove mucus on the surfaces of the fish scales, adding a sodium bicarbonate solution to remove lipid substances in the fish scales, and cleaning with deionized water;

step three: extracting by enzymolysis

Adding an enzyme preparation which is 3-8% of the weight of the fed fish scales, carrying out enzymolysis on the pretreated fish scales, adding a sodium bicarbonate solution to adjust the pH value to 5-6, then adjusting the temperature to 15-20 ℃, raising the temperature to 85 ℃ after 24 hours of enzymolysis, carrying out enzyme deactivation, and filtering the enzymolysis liquid to obtain primary collagen peptidase hydrolysate;

step four: secondary enzymolysis extraction

Grinding the fish scales filtered out in the third step into powder, adding an enzyme preparation which is 3% of the weight of the added fish scales, performing enzymolysis on the pretreated fish scales, adding a sodium bicarbonate solution to adjust the pH value to 5-6, adjusting the temperature to 15-20 ℃, raising the temperature to 85 ℃ after 24 hours of enzymolysis, deactivating enzyme, and filtering the enzymolysis liquid to obtain secondary collagen peptidase hydrolysate;

step five: purifying and concentrating

Stirring and mixing the primary collagen peptidase hydrolyzed solution and the secondary collagen peptidase hydrolyzed solution obtained in the third step and the fourth step, then performing fine filtration by using an ultrafiltration membrane, and performing cooking concentration on the collagen peptide liquid after the fine filtration to obtain a collagen peptide concentrated solution;

step six: drying

And (3) rapidly drying the collagen peptide concentrated solution by utilizing spray drying to obtain a finished collagen peptide powder product.

Preferably, the cleaning device in the first step is stirring cleaning and aeration cleaning.

Preferably, the alkali solution in the second step is sodium hydroxide or potassium hydroxide solution.

Preferably, in the fourth step, the fish scales are ground into powder by a fish scale grinding machine.

Preferably, the enzyme preparation in the third step and the fourth step comprises 3-8 parts of neutral protease, 8-14 parts of papain, 10-16 parts of pepsin, 5-7 parts of trypsin and 3-5 parts of flavourzyme.

Preferably, the enzyme preparation comprises 3 parts of neutral protease, 8 parts of papain, 10 parts of pepsin, 5 parts of trypsin and 3 parts of flavourzyme.

Preferably, the enzyme preparation comprises 3-8 parts of neutral protease, 11 parts of papain, 13 parts of pepsin, 6 parts of trypsin and 4 parts of flavourzyme.

Preferably, the enzyme preparation comprises 8 parts of neutral protease, 14 parts of papain, 16 parts of pepsin, 7 parts of trypsin and 5 parts of flavourzyme.

Compared with the prior art, the invention has the beneficial effects that: through carrying out the secondary to the fish scale and drawing, can improve the utilization ratio of collagen peptide in the fish scale, reduce extravagantly, and carry out the enzymolysis to the fish scale through multiple enzyme synergism, can improve the enzymolysis efficiency of fish scale.

Detailed Description

Example 1

A method for extracting micromolecular collagen peptide by fish scale enzymolysis comprises the following steps:

the method comprises the following steps: fish scale cleaning

The collected fish scales are poured into cleaning equipment for cleaning, so that impurities adhered to the surfaces of the fish scales can be cleaned;

step two: pretreatment of

Putting the cleaned fish scales into a reaction kettle, adding an alkali solution into the reaction kettle to enable the pH value of the solution in the reaction kettle to be 7-8, stirring for 1-2 hours to remove mucus on the surfaces of the fish scales, adding a sodium bicarbonate solution to remove lipid substances in the fish scales, and cleaning with deionized water;

step three: extracting by enzymolysis

Adding an enzyme preparation which is 3-8% of the weight of the fed fish scales, carrying out enzymolysis on the pretreated fish scales, adding a sodium bicarbonate solution to adjust the pH value to 5-6, then adjusting the temperature to 15-20 ℃, raising the temperature to 85 ℃ after 24 hours of enzymolysis, carrying out enzyme deactivation, and filtering the enzymolysis liquid to obtain primary collagen peptidase hydrolysate;

step four: secondary enzymolysis extraction

Grinding the fish scales filtered out in the third step into powder, adding an enzyme preparation which is 3% of the weight of the added fish scales, performing enzymolysis on the pretreated fish scales, adding a sodium bicarbonate solution to adjust the pH value to 5-6, adjusting the temperature to 15-20 ℃, raising the temperature to 85 ℃ after 24 hours of enzymolysis, deactivating enzyme, and filtering the enzymolysis liquid to obtain secondary collagen peptidase hydrolysate;

step five: purifying and concentrating

Stirring and mixing the primary collagen peptidase hydrolyzed solution and the secondary collagen peptidase hydrolyzed solution obtained in the third step and the fourth step, then performing fine filtration by using an ultrafiltration membrane, and performing cooking concentration on the collagen peptide liquid after the fine filtration to obtain a collagen peptide concentrated solution;

step six: drying

And (3) rapidly drying the collagen peptide concentrated solution by utilizing spray drying to obtain a finished collagen peptide powder product.

And in the step one, the cleaning equipment is used for stirring cleaning and aeration cleaning, and the scales can be dispersed during cleaning by utilizing stirring and aeration, so that the phenomenon that the scales are adhered together to influence the cleaning effect is avoided.

And in the second step, the alkali solution is sodium hydroxide or potassium hydroxide solution.

And in the fourth step, the fish scales are grinded into powder by a fish scale grinding machine.

The enzyme preparation in the third step and the fourth step comprises 3 parts of neutral protease, 8 parts of papain, 10 parts of pepsin, 5 parts of trypsin and 3 parts of flavourzyme.

Example 2

A method for extracting micromolecular collagen peptide by fish scale enzymolysis comprises the following steps:

the method comprises the following steps: fish scale cleaning

The collected fish scales are poured into cleaning equipment for cleaning, so that impurities adhered to the surfaces of the fish scales can be cleaned;

step two: pretreatment of

Putting the cleaned fish scales into a reaction kettle, adding an alkali solution into the reaction kettle to enable the pH value of the solution in the reaction kettle to be 7-8, stirring for 1-2 hours to remove mucus on the surfaces of the fish scales, adding a sodium bicarbonate solution to remove lipid substances in the fish scales, and cleaning with deionized water;

step three: extracting by enzymolysis

Adding an enzyme preparation which is 3-8% of the weight of the fed fish scales, carrying out enzymolysis on the pretreated fish scales, adding a sodium bicarbonate solution to adjust the pH value to 5-6, then adjusting the temperature to 15-20 ℃, raising the temperature to 85 ℃ after 24 hours of enzymolysis, carrying out enzyme deactivation, and filtering the enzymolysis liquid to obtain primary collagen peptidase hydrolysate;

step four: secondary enzymolysis extraction

Grinding the fish scales filtered out in the third step into powder, adding an enzyme preparation which is 3% of the weight of the added fish scales, performing enzymolysis on the pretreated fish scales, adding a sodium bicarbonate solution to adjust the pH value to 5-6, adjusting the temperature to 15-20 ℃, raising the temperature to 85 ℃ after 24 hours of enzymolysis, deactivating enzyme, and filtering the enzymolysis liquid to obtain secondary collagen peptidase hydrolysate;

step five: purifying and concentrating

Stirring and mixing the primary collagen peptidase hydrolyzed solution and the secondary collagen peptidase hydrolyzed solution obtained in the third step and the fourth step, then performing fine filtration by using an ultrafiltration membrane, and performing cooking concentration on the collagen peptide liquid after the fine filtration to obtain a collagen peptide concentrated solution;

step six: drying

And (3) rapidly drying the collagen peptide concentrated solution by utilizing spray drying to obtain a finished collagen peptide powder product.

And in the step one, the cleaning equipment is used for stirring cleaning and aeration cleaning, and the scales can be dispersed during cleaning by utilizing stirring and aeration, so that the phenomenon that the scales are adhered together to influence the cleaning effect is avoided.

And in the second step, the alkali solution is sodium hydroxide or potassium hydroxide solution.

And in the fourth step, the fish scales are grinded into powder by a fish scale grinding machine.

The enzyme preparation in the third step and the fourth step comprises 3-8 parts of neutral protease, 11 parts of papain, 13 parts of pepsin, 6 parts of trypsin and 4 parts of flavourzyme.

Embodiment 3

A method for extracting micromolecular collagen peptide by fish scale enzymolysis comprises the following steps:

the method comprises the following steps: fish scale cleaning

The collected fish scales are poured into cleaning equipment for cleaning, so that impurities adhered to the surfaces of the fish scales can be cleaned;

step two: pretreatment of

Putting the cleaned fish scales into a reaction kettle, adding an alkali solution into the reaction kettle to enable the pH value of the solution in the reaction kettle to be 7-8, stirring for 1-2 hours to remove mucus on the surfaces of the fish scales, adding a sodium bicarbonate solution to remove lipid substances in the fish scales, and cleaning with deionized water;

step three: extracting by enzymolysis

Adding an enzyme preparation which is 3-8% of the weight of the fed fish scales, carrying out enzymolysis on the pretreated fish scales, adding a sodium bicarbonate solution to adjust the pH value to 5-6, then adjusting the temperature to 15-20 ℃, raising the temperature to 85 ℃ after 24 hours of enzymolysis, carrying out enzyme deactivation, and filtering the enzymolysis liquid to obtain primary collagen peptidase hydrolysate;

step four: secondary enzymolysis extraction

Grinding the fish scales filtered out in the third step into powder, adding an enzyme preparation which is 3% of the weight of the added fish scales, performing enzymolysis on the pretreated fish scales, adding a sodium bicarbonate solution to adjust the pH value to 5-6, adjusting the temperature to 15-20 ℃, raising the temperature to 85 ℃ after 24 hours of enzymolysis, deactivating enzyme, and filtering the enzymolysis liquid to obtain secondary collagen peptidase hydrolysate;

step five: purifying and concentrating

Stirring and mixing the primary collagen peptidase hydrolyzed solution and the secondary collagen peptidase hydrolyzed solution obtained in the third step and the fourth step, then performing fine filtration by using an ultrafiltration membrane, and performing cooking concentration on the collagen peptide liquid after the fine filtration to obtain a collagen peptide concentrated solution;

step six: drying

And (3) rapidly drying the collagen peptide concentrated solution by utilizing spray drying to obtain a finished collagen peptide powder product.

And in the step one, the cleaning equipment is used for stirring cleaning and aeration cleaning, and the scales can be dispersed during cleaning by utilizing stirring and aeration, so that the phenomenon that the scales are adhered together to influence the cleaning effect is avoided.

And in the second step, the alkali solution is sodium hydroxide or potassium hydroxide solution.

And in the fourth step, the fish scales are grinded into powder by a fish scale grinding machine.

The enzyme preparation in the third step and the fourth step comprises 8 parts of neutral protease, 14 parts of papain, 16 parts of pepsin, 7 parts of trypsin and 5 parts of flavourzyme.